AlfalfaGEDB | MS.gene28545

M. sativa cultivar XinJiangDaYe / MS.gene28545

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28545.t1	GAU31768.1	67.1	158	50	2	20	175	97	254	3.40E-59	238.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28545.t1	A0A392LZ54	49.0	298	129	6	20	311	98	378	9.5e-64	253.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene28545	MS.gene94654	PPI
MS.gene28545	MS.gene26844	PPI
MS.gene28545	MS.gene64178	PPI
MS.gene28545	MS.gene055546	PPI
MS.gene28545	MS.gene55209	PPI
MS.gene28545	MS.gene96174	PPI
MS.gene28545	MS.gene074257	PPI
MS.gene28545	MS.gene04749	PPI
MS.gene20246	MS.gene28545	PPI
MS.gene28545	MS.gene073573	PPI
MS.gene28545	MS.gene28718	PPI
MS.gene28545	MS.gene44144	PPI
MS.gene28545	MS.gene060718	PPI
MS.gene28545	MS.gene63743	PPI
MS.gene28545	MS.gene016102	PPI
MS.gene28545	MS.gene055223	PPI
MS.gene28545	MS.gene068629	PPI
MS.gene28545	MS.gene08106	PPI
MS.gene28545	MS.gene004417	PPI
MS.gene28545	MS.gene009781	PPI
MS.gene28545	MS.gene031967	PPI
MS.gene042559	MS.gene28545	PPI
MS.gene28545	MS.gene21066	PPI
MS.gene28545	MS.gene009213	PPI
MS.gene28545	MS.gene013890	PPI
MS.gene28545	MS.gene98127	PPI
MS.gene28545	MS.gene003264	PPI
MS.gene28545	MS.gene054652	PPI
MS.gene28545	MS.gene000634	PPI
MS.gene69490	MS.gene28545	PPI
MS.gene015248	MS.gene28545	PPI
MS.gene28545	MS.gene86849	PPI
MS.gene34812	MS.gene28545	PPI
MS.gene28545	MS.gene42903	PPI
MS.gene28545	MS.gene80362	PPI
MS.gene28545	MS.gene49057	PPI
MS.gene28545	MS.gene89815	PPI
MS.gene28545	MS.gene018474	PPI
MS.gene28545	MS.gene048088	PPI
MS.gene28545	MS.gene86868	PPI
MS.gene067340	MS.gene28545	PPI
MS.gene28545	MS.gene058889	PPI
MS.gene28545	MS.gene96163	PPI
MS.gene28545	MS.gene034643	PPI
MS.gene28545	MS.gene60059	PPI
MS.gene05357	MS.gene28545	PPI
MS.gene28545	MS.gene050679	PPI
MS.gene28545	MS.gene012889	PPI
MS.gene28545	MS.gene34463	PPI
MS.gene28545	MS.gene038757	PPI
MS.gene28545	MS.gene015248	PPI
MS.gene28545	MS.gene030090	PPI
MS.gene28545	MS.gene61499	PPI
MS.gene28545	MS.gene51804	PPI
MS.gene28545	MS.gene013644	PPI
MS.gene28545	MS.gene05358	PPI
MS.gene28545	MS.gene025675	PPI
MS.gene28545	MS.gene69495	PPI
MS.gene28545	MS.gene04970	PPI
MS.gene28545	MS.gene71637	PPI
MS.gene28545	MS.gene26038	PPI
MS.gene28545	MS.gene43924	PPI
MS.gene28545	MS.gene022929	PPI
MS.gene28545	MS.gene85295	PPI
MS.gene28545	MS.gene26433	PPI
MS.gene28545	MS.gene007022	PPI
MS.gene42903	MS.gene28545	PPI
MS.gene28545	MS.gene69584	PPI
MS.gene28545	MS.gene52349	PPI
MS.gene28545	MS.gene021647	PPI
MS.gene28545	MS.gene047954	PPI
MS.gene28545	MS.gene30136	PPI
MS.gene28545	MS.gene65471	PPI
MS.gene28545	MS.gene071784	PPI
MS.gene28545	MS.gene048342	PPI
MS.gene22200	MS.gene28545	PPI
MS.gene28545	MS.gene069624	PPI
MS.gene28545	MS.gene060386	PPI
MS.gene28545	MS.gene83979	PPI
MS.gene28545	MS.gene073582	PPI
MS.gene94654	MS.gene28545	PPI
MS.gene28545	MS.gene63762	PPI
MS.gene28545	MS.gene016719	PPI
MS.gene28545	MS.gene048072	PPI
MS.gene28545	MS.gene028714	PPI
MS.gene28545	MS.gene88393	PPI
MS.gene28545	MS.gene042027	PPI
MS.gene28545	MS.gene048706	PPI
MS.gene28545	MS.gene69586	PPI
MS.gene28545	MS.gene45083	PPI
MS.gene28545	MS.gene049561	PPI
MS.gene28545	MS.gene008658	PPI
MS.gene28545	MS.gene47792	PPI
MS.gene063214	MS.gene28545	PPI
MS.gene28545	MS.gene63150	PPI
MS.gene035626	MS.gene28545	PPI
MS.gene28545	MS.gene03936	PPI
MS.gene28545	MS.gene43393	PPI
MS.gene28545	MS.gene000629	PPI
MS.gene28545	MS.gene072852	PPI
MS.gene28545	MS.gene08708	PPI
MS.gene30208	MS.gene28545	PPI
MS.gene69495	MS.gene28545	PPI
MS.gene28545	MS.gene06164	PPI
MS.gene28545	MS.gene50392	PPI
MS.gene28545	MS.gene86275	PPI
MS.gene28545	MS.gene22730	PPI
MS.gene28545	MS.gene017586	PPI
MS.gene28545	MS.gene038098	PPI
MS.gene28545	MS.gene037634	PPI
MS.gene28545	MS.gene057828	PPI
MS.gene28545	MS.gene071320	PPI
MS.gene95561	MS.gene28545	PPI
MS.gene28545	MS.gene08606	PPI
MS.gene28545	MS.gene012019	PPI
MS.gene28545	MS.gene46941	PPI
MS.gene28545	MS.gene048747	PPI
MS.gene28545	MS.gene000640	PPI
MS.gene065627	MS.gene28545	PPI
MS.gene28545	MS.gene000506	PPI
MS.gene28545	MS.gene061850	PPI
MS.gene28545	MS.gene98756	PPI
MS.gene57019	MS.gene28545	PPI
MS.gene28545	MS.gene39956	PPI
MS.gene28545	MS.gene028716	PPI
MS.gene28545	MS.gene74639	PPI
MS.gene28545	MS.gene071133	PPI
MS.gene28545	MS.gene64130	PPI
MS.gene28545	MS.gene60266	PPI
MS.gene28545	MS.gene98467	PPI
MS.gene021968	MS.gene28545	PPI
MS.gene28545	MS.gene024522	PPI
MS.gene28545	MS.gene54051	PPI
MS.gene28545	MS.gene22111	PPI
MS.gene28545	MS.gene049610	PPI
MS.gene28545	MS.gene010290	PPI
MS.gene28545	MS.gene93396	PPI
MS.gene28545	MS.gene37955	PPI
MS.gene28545	MS.gene95561	PPI
MS.gene28545	MS.gene92517	PPI
MS.gene28545	MS.gene34197	PPI
MS.gene28545	MS.gene36069	PPI
MS.gene28545	MS.gene045875	PPI
MS.gene28545	MS.gene010890	PPI
MS.gene28545	MS.gene84790	PPI
MS.gene28545	MS.gene71675	PPI
MS.gene28545	MS.gene055683	PPI
MS.gene008275	MS.gene28545	PPI
MS.gene28545	MS.gene061579	PPI
MS.gene28545	MS.gene05319	PPI
MS.gene28545	MS.gene039692	PPI
MS.gene28545	MS.gene032311	PPI
MS.gene28545	MS.gene002590	PPI
MS.gene28545	MS.gene41979	PPI
MS.gene28545	MS.gene008927	PPI
MS.gene28545	MS.gene060788	PPI
MS.gene28545	MS.gene98616	PPI
MS.gene28545	MS.gene23521	PPI
MS.gene28545	MS.gene41643	PPI
MS.gene28545	MS.gene77990	PPI
MS.gene28545	MS.gene65546	PPI
MS.gene28545	MS.gene062511	PPI
MS.gene28545	MS.gene008861	PPI
MS.gene28545	MS.gene025873	PPI
MS.gene28545	MS.gene065627	PPI
MS.gene28545	MS.gene027994	PPI
MS.gene28545	MS.gene059269	PPI
MS.gene28545	MS.gene96541	PPI
MS.gene28545	MS.gene045614	PPI
MS.gene28545	MS.gene064966	PPI
MS.gene28545	MS.gene036423	PPI
MS.gene28545	MS.gene76420	PPI
MS.gene28545	MS.gene46353	PPI
MS.gene96109	MS.gene28545	PPI
MS.gene28545	MS.gene51799	PPI
MS.gene28545	MS.gene98471	PPI
MS.gene28545	MS.gene062067	PPI
MS.gene28545	MS.gene61651	PPI
MS.gene28545	MS.gene013676	PPI
MS.gene28545	MS.gene29406	PPI
MS.gene28545	MS.gene09023	PPI
MS.gene28545	MS.gene021968	PPI
MS.gene28545	MS.gene054508	PPI
MS.gene28545	MS.gene025987	PPI
MS.gene28545	MS.gene95960	PPI
MS.gene28545	MS.gene053373	PPI
MS.gene28545	MS.gene043140	PPI
MS.gene28545	MS.gene041264	PPI
MS.gene28545	MS.gene35175	PPI
MS.gene28545	MS.gene042559	PPI
MS.gene28545	MS.gene035626	PPI
MS.gene28545	MS.gene46948	PPI
MS.gene016102	MS.gene28545	PPI
MS.gene46941	MS.gene28545	PPI
MS.gene96541	MS.gene28545	PPI
MS.gene28545	MS.gene44083	PPI
MS.gene28545	MS.gene29321	PPI
MS.gene28545	MS.gene65474	PPI
MS.gene28545	MS.gene018745	PPI
MS.gene28545	MS.gene025401	PPI
MS.gene28545	MS.gene34812	PPI
MS.gene28545	MS.gene57044	PPI
MS.gene28545	MS.gene015093	PPI
MS.gene025873	MS.gene28545	PPI
MS.gene28545	MS.gene04134	PPI
MS.gene28545	MS.gene24278	PPI
MS.gene28545	MS.gene000379	PPI
MS.gene28545	MS.gene34618	PPI
MS.gene28545	MS.gene032299	PPI
MS.gene05358	MS.gene28545	PPI
MS.gene28545	MS.gene009121	PPI
MS.gene28545	MS.gene08713	PPI
MS.gene28545	MS.gene70932	PPI
MS.gene28545	MS.gene09517	PPI
MS.gene28545	MS.gene87845	PPI
MS.gene28545	MS.gene57019	PPI
MS.gene28545	MS.gene32415	PPI
MS.gene28545	MS.gene85308	PPI
MS.gene28545	MS.gene62249	PPI
MS.gene28545	MS.gene58419	PPI
MS.gene56991	MS.gene28545	PPI
MS.gene28545	MS.gene046089	PPI
MS.gene28545	MS.gene053747	PPI
MS.gene28545	MS.gene69490	PPI
MS.gene28545	MS.gene021555	PPI
MS.gene28545	MS.gene05576	PPI
MS.gene28545	MS.gene044729	PPI
MS.gene28545	MS.gene96109	PPI
MS.gene28545	MS.gene008275	PPI
MS.gene28545	MS.gene60390	PPI
MS.gene28545	MS.gene48477	PPI
MS.gene28545	MS.gene027169	PPI
MS.gene28545	MS.gene55682	PPI
MS.gene28545	MS.gene81712	PPI
MS.gene28545	MS.gene019177	PPI
MS.gene28545	MS.gene05436	PPI
MS.gene28545	MS.gene038623	PPI
MS.gene28545	MS.gene005120	PPI
MS.gene28545	MS.gene055560	PPI
MS.gene28545	MS.gene05357	PPI
MS.gene28545	MS.gene024297	PPI
MS.gene28545	MS.gene35202	PPI
MS.gene28545	MS.gene22200	PPI
MS.gene28545	MS.gene057934	PPI
MS.gene28545	MS.gene37537	PPI
MS.gene28545	MS.gene024841	PPI
MS.gene28545	MS.gene20246	PPI
MS.gene28545	MS.gene069786	PPI
MS.gene28545	MS.gene42010	PPI
MS.gene28545	MS.gene025158	PPI
MS.gene28545	MS.gene049512	PPI
MS.gene28545	MS.gene40828	PPI
MS.gene72520	MS.gene28545	PPI
MS.gene28545	MS.gene27329	PPI
MS.gene013193	MS.gene28545	PPI
MS.gene28545	MS.gene87529	PPI
MS.gene28545	MS.gene41393	PPI
MS.gene08106	MS.gene28545	PPI
MS.gene28545	MS.gene30208	PPI
MS.gene28545	MS.gene97410	PPI
MS.gene28545	MS.gene56991	PPI
MS.gene28545	MS.gene010656	PPI
MS.gene28545	MS.gene016632	PPI
MS.gene28545	MS.gene013193	PPI
MS.gene28545	MS.gene40469	PPI
MS.gene28545	MS.gene65623	PPI
MS.gene28545	MS.gene012748	PPI
MS.gene28545	MS.gene063214	PPI
MS.gene28545	MS.gene66651	PPI
MS.gene28545	MS.gene02896	PPI
MS.gene28545	MS.gene71588	PPI
MS.gene28545	MS.gene72520	PPI
MS.gene28545	MS.gene61452	PPI
MS.gene28545	MS.gene05231	PPI
MS.gene28545	MS.gene03149	PPI
MS.gene28545	MS.gene037049	PPI
MS.gene28545	MS.gene042614	PPI
MS.gene28545	MS.gene026680	PPI
MS.gene038676	MS.gene28545	PPI
MS.gene28545	MS.gene051449	PPI
MS.gene28545	MS.gene042651	PPI
MS.gene28545	MS.gene59460	PPI
MS.gene28545	MS.gene019165	PPI
MS.gene28545	MS.gene81194	PPI
MS.gene28545	MS.gene060773	PPI
MS.gene93396	MS.gene28545	PPI
MS.gene28545	MS.gene66650	PPI
MS.gene28545	MS.gene038394	PPI
MS.gene28545	MS.gene09589	PPI
MS.gene28545	MS.gene019001	PPI
MS.gene28545	MS.gene024405	PPI
MS.gene28545	MS.gene067340	PPI
MS.gene28545	MS.gene021965	PPI
MS.gene28545	MS.gene018196	PPI
MS.gene28545	MS.gene038676	PPI
MS.gene28545	MS.gene53904	PPI
MS.gene28545	MS.gene24070	PPI
MS.gene28545	MS.gene83772	PPI
MS.gene28545	MS.gene73261	PPI
MS.gene28545	MS.gene059736	PPI
MS.gene28545	MS.gene85538	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28545.t1	MTR_0010s0370	59.494	79	29	1	29	107	109	184	3.50e-26	102
MS.gene28545.t1	MTR_0010s0350	59.494	79	29	1	29	107	109	184	3.50e-26	102
MS.gene28545.t1	MTR_7g089990	44.340	106	57	1	26	129	88	193	3.71e-20	87.4
MS.gene28545.t1	MTR_6g033215	36.842	95	60	0	25	119	200	294	3.57e-15	75.1
MS.gene28545.t1	MTR_1g036710	34.483	116	42	1	52	167	1	82	6.75e-15	73.9
MS.gene28545.t1	MTR_8g468010	39.344	61	37	0	35	95	1	61	1.15e-12	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 85 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAAACGATATATGAGAATT+AGG	0.158072	8.4:+27622078	MS.gene28545:CDS
ATGATGATGGAATTAGAAAT+TGG	0.236672	8.4:+27622270	MS.gene28545:CDS
TGTTATTACTGTGGTATGTT+AGG	0.286591	8.4:+27622206	MS.gene28545:CDS
TTTATTTGTGCCTCCCTTGA+TGG	0.297105	8.4:-27622596	None:intergenic
CAATTTACCGGTGGGCTATA+TGG	0.301067	8.4:+27621977	MS.gene28545:CDS
TTTATCTCCACTCGGATATC+TGG	0.301906	8.4:-27622296	None:intergenic
GCATATGATGCCGTAATTAA+TGG	0.319451	8.4:+27622416	MS.gene28545:CDS
AAAATCCTTTCCTTCTTCAA+TGG	0.331165	8.4:-27622545	None:intergenic
GATTCAAGTACACAATTTAC+CGG	0.339904	8.4:+27621965	MS.gene28545:CDS
TGCTAAGTACAGTCCCGTTT+TGG	0.343263	8.4:+27621943	MS.gene28545:CDS
CTGTGGTATGTTAGGTCATA+TGG	0.344261	8.4:+27622214	MS.gene28545:CDS
GAAACGATATATGAGAATTA+GGG	0.360173	8.4:+27622079	MS.gene28545:CDS
TCCACTTATGCCATTAATTA+CGG	0.363101	8.4:-27622426	None:intergenic
TACTTAATGTTAACTAAAAC+AGG	0.364527	8.4:-27622164	None:intergenic
GTACTTAGCATGACATGTTC+TGG	0.370251	8.4:-27621930	None:intergenic
GAGAATCATATGCAAATTGA+TGG	0.373960	8.4:+27622731	MS.gene28545:CDS
TGATGATGGAATTAGAAATT+GGG	0.388458	8.4:+27622271	MS.gene28545:CDS
AAATCCTTTCCTTCTTCAAT+GGG	0.414749	8.4:-27622544	None:intergenic
GGTAGTGTGATAGATAATTT+CGG	0.431271	8.4:+27622692	MS.gene28545:CDS
GCCATGGAACACCTACGATT+GGG	0.434964	8.4:+27621603	None:intergenic
GGAAGTAGATGGGCGGTAGA+AGG	0.441460	8.4:+27622338	MS.gene28545:CDS
ACCATGATGGCGGAAGTAGA+TGG	0.447409	8.4:+27622327	MS.gene28545:CDS
GATAAGAAAAGGATGCTTGA+TGG	0.447478	8.4:+27622656	MS.gene28545:CDS
TGTGTACTTGAATCCAAAAC+GGG	0.452919	8.4:-27621956	None:intergenic
GCACAAATAAATATGACAAC+TGG	0.468581	8.4:+27622608	MS.gene28545:CDS
CTTGAGTATGACGAGAAGAA+TGG	0.471427	8.4:+27622044	MS.gene28545:CDS
TGAAATACCGGAGGAAGAAC+TGG	0.471773	8.4:+27621644	MS.gene28545:CDS
ATATATGAGAATTAGGGTTA+TGG	0.472699	8.4:+27622085	MS.gene28545:CDS
CCCTAGCATAACTGATCCAT+TGG	0.478486	8.4:-27622465	None:intergenic
TTGTGTACTTGAATCCAAAA+CGG	0.478820	8.4:-27621957	None:intergenic
TAGTGGAATCTGATAAGAAA+AGG	0.490455	8.4:+27622645	MS.gene28545:CDS
TGGTGTAGAGAAGGAAATAG+TGG	0.490769	8.4:+27622628	MS.gene28545:CDS
GCCGTAATTAATGGCATAAG+TGG	0.492350	8.4:+27622425	MS.gene28545:CDS
GGGCTATATGGCGGAAACGG+TGG	0.499185	8.4:+27621989	MS.gene28545:CDS
CCCATCTACTTCCGCCATCA+TGG	0.503448	8.4:-27622328	None:intergenic
AGACGATGAGTTTGAAATAC+CGG	0.511419	8.4:+27621632	MS.gene28545:CDS
GGCTATATGGCGGAAACGGT+GGG	0.520290	8.4:+27621990	MS.gene28545:CDS
GTATGACGAGAAGAATGGTT+CGG	0.521327	8.4:+27622049	MS.gene28545:CDS
CCATGATGGCGGAAGTAGAT+GGG	0.523617	8.4:+27622328	MS.gene28545:CDS
AAAGTATTGAACAACCATCA+AGG	0.525685	8.4:+27622582	MS.gene28545:CDS
AAAGATGAACCCATTGAAGA+AGG	0.527300	8.4:+27622535	MS.gene28545:CDS
CCCCAATCGTAGGTGTTCCA+TGG	0.531635	8.4:-27621604	None:intergenic
GAACTGGTGCTAGGCGGAGT+AGG	0.535223	8.4:+27621660	MS.gene28545:CDS
AGCCATGGAACACCTACGAT+TGG	0.535444	8.4:+27621602	None:intergenic
GGTGGGCTATATGGCGGAAA+CGG	0.537525	8.4:+27621986	MS.gene28545:CDS
CCAATGGATCAGTTATGCTA+GGG	0.543299	8.4:+27622465	MS.gene28545:CDS
TTCTTCGATTTATCTCCACT+CGG	0.546410	8.4:-27622304	None:intergenic
CCTAGCACCAGTTCTTCCTC+CGG	0.547288	8.4:-27621651	None:intergenic
TGAACCCATTGAAGAAGGAA+AGG	0.558569	8.4:+27622540	MS.gene28545:CDS
TATGAGAATTAGGGTTATGG+TGG	0.559714	8.4:+27622088	MS.gene28545:CDS
TATGACAACTGGTGTAGAGA+AGG	0.561145	8.4:+27622619	MS.gene28545:CDS
ACCAATGGATCAGTTATGCT+AGG	0.566399	8.4:+27622464	MS.gene28545:CDS
TAAGAACGTTGGAAACTATG+TGG	0.570806	8.4:+27622013	MS.gene28545:CDS
TAGTTAACATTAAGTATGAA+CGG	0.572760	8.4:+27622171	MS.gene28545:CDS
CAAGTACACAATTTACCGGT+GGG	0.573700	8.4:+27621969	MS.gene28545:CDS
TTAGGGTTATGGTGGATGTG+AGG	0.573990	8.4:+27622096	MS.gene28545:CDS
TATGTCGTTGAAGATGATGA+TGG	0.574296	8.4:+27622257	MS.gene28545:CDS
GCTTGATGGTAGAAGCAAGA+AGG	0.574888	8.4:+27622670	MS.gene28545:CDS
GCTGCAAGTTCTATCACCAA+TGG	0.584448	8.4:+27622449	MS.gene28545:CDS
CCATGGAACACCTACGATTG+GGG	0.588731	8.4:+27621604	None:intergenic
CTTGATGGTAGAAGCAAGAA+GGG	0.592619	8.4:+27622671	MS.gene28545:CDS
CCGGAGGAAGAACTGGTGCT+AGG	0.593081	8.4:+27621651	MS.gene28545:CDS
AAGAACGTTGGAAACTATGT+GGG	0.595232	8.4:+27622014	MS.gene28545:CDS
AAGTATTGAACAACCATCAA+GGG	0.597973	8.4:+27622583	MS.gene28545:CDS
TCAAGTACACAATTTACCGG+TGG	0.613485	8.4:+27621968	MS.gene28545:CDS
ACACAAGCAGAAGGACATGA+TGG	0.621944	8.4:+27622490	MS.gene28545:CDS
GGTGGATGTGAGGAAGCCGT+TGG	0.625357	8.4:+27622106	MS.gene28545:CDS
GATGATGGAATTAGAAATTG+GGG	0.627755	8.4:+27622272	MS.gene28545:CDS
AACATTAAGTATGAACGGCT+AGG	0.633354	8.4:+27622176	MS.gene28545:CDS
GAAACGGTGGGTAAGAACGT+TGG	0.653921	8.4:+27622002	MS.gene28545:CDS
GCTAGGGGAACACAAGCAGA+AGG	0.654573	8.4:+27622481	MS.gene28545:CDS
ATAAATCGAAGAAACCATGA+TGG	0.656330	8.4:+27622314	MS.gene28545:CDS
AATCGAAGAAACCATGATGG+CGG	0.663196	8.4:+27622317	MS.gene28545:CDS
TTTACCGGTGGGCTATATGG+CGG	0.675964	8.4:+27621980	MS.gene28545:CDS
CATGGAACACCTACGATTGG+GGG	0.678429	8.4:+27621605	None:intergenic
CAATGGATCAGTTATGCTAG+GGG	0.682446	8.4:+27622466	MS.gene28545:CDS
AGAACGTTGGAAACTATGTG+GGG	0.683015	8.4:+27622015	MS.gene28545:CDS
CGATGAGTTTGAAATACCGG+AGG	0.687651	8.4:+27621635	MS.gene28545:CDS
TGGTATGTTAGGTCATATGG+AGG	0.695628	8.4:+27622217	MS.gene28545:CDS
TATTGAACAACCATCAAGGG+AGG	0.699859	8.4:+27622586	MS.gene28545:CDS
GAGGAAGAACTGGTGCTAGG+CGG	0.703735	8.4:+27621654	MS.gene28545:CDS
TGATGGCGGAAGTAGATGGG+CGG	0.718209	8.4:+27622331	MS.gene28545:CDS
GTTTCCGCCATATAGCCCAC+CGG	0.719594	8.4:-27621984	None:intergenic
GAACGTTGGAAACTATGTGG+GGG	0.723392	8.4:+27622016	MS.gene28545:CDS
TTGGGGTCCAGATATCCGAG+TGG	0.769645	8.4:+27622289	MS.gene28545:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTCTCAAAATTTTATTAATA+TGG	+	chr8.4:27623109-27623128	MS.gene28545:intron	10.0%
!!!	GCAAAATATTCATTTTTTAT+CGG	+	chr8.4:27622808-27622827	MS.gene28545:intron	15.0%
!!!	TCAAAATTTTATTAATATGG+AGG	+	chr8.4:27623112-27623131	MS.gene28545:intron	15.0%
!!	TAATAAGATTGCAATCAAAA+GGG	-	chr8.4:27623085-27623104	None:intergenic	20.0%
!!	TACTTAATGTTAACTAAAAC+AGG	-	chr8.4:27622167-27622186	None:intergenic	20.0%
!!	TAGTTAACATTAAGTATGAA+CGG	+	chr8.4:27622171-27622190	MS.gene28545:CDS	20.0%
!!	TTAATAAGATTGCAATCAAA+AGG	-	chr8.4:27623086-27623105	None:intergenic	20.0%
!!!	CTAAAAGTATCAAAAAGAAA+GGG	+	chr8.4:27622135-27622154	MS.gene28545:CDS	20.0%
!!!	TAAAAGTATCAAAAAGAAAG+GGG	+	chr8.4:27622136-27622155	MS.gene28545:CDS	20.0%
!	ATATATGAGAATTAGGGTTA+TGG	+	chr8.4:27622085-27622104	MS.gene28545:CDS	25.0%
!	GAAACGATATATGAGAATTA+GGG	+	chr8.4:27622079-27622098	MS.gene28545:CDS	25.0%
!!!	ATGATGATGGAATTAGAAAT+TGG	+	chr8.4:27622270-27622289	MS.gene28545:CDS	25.0%
!!!	GCTAAAAGTATCAAAAAGAA+AGG	+	chr8.4:27622134-27622153	MS.gene28545:CDS	25.0%
!!!	TCCTTGTGTTGATTTTTTAT+CGG	-	chr8.4:27622375-27622394	None:intergenic	25.0%
!!!	TGATGATGGAATTAGAAATT+GGG	+	chr8.4:27622271-27622290	MS.gene28545:CDS	25.0%
	AAAATCCTTTCCTTCTTCAA+TGG	-	chr8.4:27622548-27622567	None:intergenic	30.0%
	AAATCCTTTCCTTCTTCAAT+GGG	-	chr8.4:27622547-27622566	None:intergenic	30.0%
	AATATCTAGTCACTAGTGAA+GGG	+	chr8.4:27621765-27621784	MS.gene28545:intron	30.0%
	AGAAAGAATTGATAGAGCTA+TGG	+	chr8.4:27623439-27623458	MS.gene28545:intron	30.0%
	ATAAATCGAAGAAACCATGA+TGG	+	chr8.4:27622314-27622333	MS.gene28545:CDS	30.0%
	CCATTGTTTCACAAAGAAAA+AGG	-	chr8.4:27622957-27622976	None:intergenic	30.0%
	CGTGGAAATAAGAGAAATTA+AGG	+	chr8.4:27621788-27621807	MS.gene28545:intron	30.0%
	GAAATAAGAGAAATTAAGGC+GGG	+	chr8.4:27621792-27621811	MS.gene28545:intron	30.0%
	GAAGAAATTAGAGTCAGATT+GGG	+	chr8.4:27622994-27623013	MS.gene28545:intron	30.0%
	GAGAATCATATGCAAATTGA+TGG	+	chr8.4:27622731-27622750	MS.gene28545:CDS	30.0%
	GATAAAAAATCAACACAAGG+AGG	+	chr8.4:27622374-27622393	MS.gene28545:CDS	30.0%
	GATTCAAGTACACAATTTAC+CGG	+	chr8.4:27621965-27621984	MS.gene28545:CDS	30.0%
	GCACAAATAAATATGACAAC+TGG	+	chr8.4:27622608-27622627	MS.gene28545:CDS	30.0%
	GGAAACGATATATGAGAATT+AGG	+	chr8.4:27622078-27622097	MS.gene28545:CDS	30.0%
	GGAAATAAGAGAAATTAAGG+CGG	+	chr8.4:27621791-27621810	MS.gene28545:intron	30.0%
	GGTAGTGTGATAGATAATTT+CGG	+	chr8.4:27622692-27622711	MS.gene28545:CDS	30.0%
	TAGTGGAATCTGATAAGAAA+AGG	+	chr8.4:27622645-27622664	MS.gene28545:CDS	30.0%
	TCATCAACGTGATATTCAAA+AGG	+	chr8.4:27621836-27621855	MS.gene28545:intron	30.0%
	TCCACTTATGCCATTAATTA+CGG	-	chr8.4:27622429-27622448	None:intergenic	30.0%
	TCCGATAAAAAATCAACACA+AGG	+	chr8.4:27622371-27622390	MS.gene28545:CDS	30.0%
	TGAAGAAATTAGAGTCAGAT+TGG	+	chr8.4:27622993-27623012	MS.gene28545:intron	30.0%
	TGTTATTACTGTGGTATGTT+AGG	+	chr8.4:27622206-27622225	MS.gene28545:CDS	30.0%
	TTGATAGATCTTCATTTGAC+AGG	+	chr8.4:27623368-27623387	MS.gene28545:intron	30.0%
	TTGTGTACTTGAATCCAAAA+CGG	-	chr8.4:27621960-27621979	None:intergenic	30.0%
!	ATGTGTTTGTTGAAAAACCA+AGG	-	chr8.4:27621876-27621895	None:intergenic	30.0%
!	CCTTTTTCTTTGTGAAACAA+TGG	+	chr8.4:27622954-27622973	MS.gene28545:intron	30.0%
!	TTTTGATGCAAATTTGTGTC+TGG	+	chr8.4:27621683-27621702	MS.gene28545:intron	30.0%
!!	AAAGTATTGAACAACCATCA+AGG	+	chr8.4:27622582-27622601	MS.gene28545:CDS	30.0%
!!	AAGTATTGAACAACCATCAA+GGG	+	chr8.4:27622583-27622602	MS.gene28545:CDS	30.0%
!!	GATATTCAAAAGGTATTGAG+AGG	+	chr8.4:27621846-27621865	MS.gene28545:intron	30.0%
!!	GATGATGGAATTAGAAATTG+GGG	+	chr8.4:27622272-27622291	MS.gene28545:CDS	30.0%
!!	TGGAGACTTACATGTTTTTA+TGG	+	chr8.4:27623158-27623177	MS.gene28545:intron	30.0%
	AAAAAATCAACACAAGGAGG+AGG	+	chr8.4:27622377-27622396	MS.gene28545:CDS	35.0%
	AAACATGTAAGTCTCCACTT+AGG	-	chr8.4:27623155-27623174	None:intergenic	35.0%
	AAAGATGAACCCATTGAAGA+AGG	+	chr8.4:27622535-27622554	MS.gene28545:CDS	35.0%
	AACAAACACATGTTGATCCT+GGG	+	chr8.4:27621885-27621904	MS.gene28545:intron	35.0%
	AACATTAAGTATGAACGGCT+AGG	+	chr8.4:27622176-27622195	MS.gene28545:CDS	35.0%
	AAGAACGTTGGAAACTATGT+GGG	+	chr8.4:27622014-27622033	MS.gene28545:CDS	35.0%
	AAGAGGAAGTCAAGATTTGA+AGG	+	chr8.4:27623415-27623434	MS.gene28545:intron	35.0%
	AAGTCAACTGATGAAGTACA+TGG	+	chr8.4:27622776-27622795	MS.gene28545:intron	35.0%
	AAGTCTCCACTTAGGATAAT+GGG	-	chr8.4:27623147-27623166	None:intergenic	35.0%
	AGTCAGAACTCATATGCTAA+AGG	+	chr8.4:27621731-27621750	MS.gene28545:intron	35.0%
	ATGCAAATTTGTGTCTGGTT+AGG	+	chr8.4:27621688-27621707	MS.gene28545:intron	35.0%
	CTTCCTTTGACCAAAGTAAA+CGG	-	chr8.4:27623398-27623417	None:intergenic	35.0%
	GAATATCTAGTCACTAGTGA+AGG	+	chr8.4:27621764-27621783	MS.gene28545:intron	35.0%
	GATAAGAAAAGGATGCTTGA+TGG	+	chr8.4:27622656-27622675	MS.gene28545:CDS	35.0%
	GCATATGATGCCGTAATTAA+TGG	+	chr8.4:27622416-27622435	MS.gene28545:CDS	35.0%
	GTTACCCAGAAAATGAAAGA+AGG	+	chr8.4:27623180-27623199	MS.gene28545:intron	35.0%
	TAAGAACGTTGGAAACTATG+TGG	+	chr8.4:27622013-27622032	MS.gene28545:CDS	35.0%
	TAAGTCTCCACTTAGGATAA+TGG	-	chr8.4:27623148-27623167	None:intergenic	35.0%
	TATCCGTTTACTTTGGTCAA+AGG	+	chr8.4:27623392-27623411	MS.gene28545:intron	35.0%
	TATGAGAATTAGGGTTATGG+TGG	+	chr8.4:27622088-27622107	MS.gene28545:CDS	35.0%
	TATGTCGTTGAAGATGATGA+TGG	+	chr8.4:27622257-27622276	MS.gene28545:CDS	35.0%
	TGTGTACTTGAATCCAAAAC+GGG	-	chr8.4:27621959-27621978	None:intergenic	35.0%
	TTCTTCGATTTATCTCCACT+CGG	-	chr8.4:27622307-27622326	None:intergenic	35.0%
	TTTACTTTGGTCAAAGGAAG+AGG	+	chr8.4:27623398-27623417	MS.gene28545:intron	35.0%
!	AGACGATGAGTTTGAAATAC+CGG	+	chr8.4:27621632-27621651	MS.gene28545:CDS	35.0%
!	ATACTTTTAGCGCAAACCAA+CGG	-	chr8.4:27622125-27622144	None:intergenic	35.0%
!	GTTGATAAAATCGGAAGAAG+CGG	+	chr8.4:27623036-27623055	MS.gene28545:intron	35.0%
!	GTTTCATGATCTGGAATTGT+CGG	+	chr8.4:27622866-27622885	MS.gene28545:intron	35.0%
!	TTTAGCATATGAGTTCTGAC+TGG	-	chr8.4:27621732-27621751	None:intergenic	35.0%
!	TTTCATGATCTGGAATTGTC+GGG	+	chr8.4:27622867-27622886	MS.gene28545:intron	35.0%
!!	AGAAGAATGGTTCGGATTTT+TGG	+	chr8.4:27622057-27622076	MS.gene28545:CDS	35.0%
!!	ATTCAAAAGGTATTGAGAGG+AGG	+	chr8.4:27621849-27621868	MS.gene28545:intron	35.0%
!!	TCGTTTGCGGTTGATAAAAT+CGG	+	chr8.4:27623027-27623046	MS.gene28545:intron	35.0%
!!	TTCAAAAGGTATTGAGAGGA+GGG	+	chr8.4:27621850-27621869	MS.gene28545:intron	35.0%
!!!	GGCATCTTTTGTTATTACTG+TGG	+	chr8.4:27622197-27622216	MS.gene28545:CDS	35.0%
	AAGTCTCCCATAATACACCA+TGG	-	chr8.4:27623254-27623273	None:intergenic	40.0%
	AATCGAAGAAACCATGATGG+CGG	+	chr8.4:27622317-27622336	MS.gene28545:CDS	40.0%
	ACCAATGGATCAGTTATGCT+AGG	+	chr8.4:27622464-27622483	MS.gene28545:CDS	40.0%
	ACCCAGAAAATGAAAGAAGG+AGG	+	chr8.4:27623183-27623202	MS.gene28545:intron	40.0%
	AGAACGTTGGAAACTATGTG+GGG	+	chr8.4:27622015-27622034	MS.gene28545:CDS	40.0%
	AGTCTCCACTTAGGATAATG+GGG	-	chr8.4:27623146-27623165	None:intergenic	40.0%
	ATCATGAAACTCATTGCTCC+CGG	-	chr8.4:27622856-27622875	None:intergenic	40.0%
	CAACAAACACATGTTGATCC+TGG	+	chr8.4:27621884-27621903	MS.gene28545:intron	40.0%
	CAAGTACACAATTTACCGGT+GGG	+	chr8.4:27621969-27621988	MS.gene28545:CDS	40.0%
	CAATGGATCAGTTATGCTAG+GGG	+	chr8.4:27622466-27622485	MS.gene28545:CDS	40.0%
	CACAAGATAATGCACTTCCA+TGG	+	chr8.4:27623234-27623253	MS.gene28545:intron	40.0%
	CCAATGGATCAGTTATGCTA+GGG	+	chr8.4:27622465-27622484	MS.gene28545:CDS	40.0%
	CCCAGAAAATGAAAGAAGGA+GGG	+	chr8.4:27623184-27623203	MS.gene28545:intron	40.0%
	CTGTGGTATGTTAGGTCATA+TGG	+	chr8.4:27622214-27622233	MS.gene28545:CDS	40.0%
	CTTGAGTATGACGAGAAGAA+TGG	+	chr8.4:27622044-27622063	MS.gene28545:CDS	40.0%
	GACAGGATATCCGTTTACTT+TGG	+	chr8.4:27623385-27623404	MS.gene28545:intron	40.0%
	GACATCTAAACGAAACTTGC+GGG	-	chr8.4:27622935-27622954	None:intergenic	40.0%
	GAGCAATGAGTTTCATGATC+TGG	+	chr8.4:27622857-27622876	MS.gene28545:intron	40.0%
	GCACTTCCATGGTGTATTAT+GGG	+	chr8.4:27623245-27623264	MS.gene28545:intron	40.0%
	GCCGTAATTAATGGCATAAG+TGG	+	chr8.4:27622425-27622444	MS.gene28545:CDS	40.0%
	GGACATCTAAACGAAACTTG+CGG	-	chr8.4:27622936-27622955	None:intergenic	40.0%
	GTACTTAGCATGACATGTTC+TGG	-	chr8.4:27621933-27621952	None:intergenic	40.0%
	GTATGACGAGAAGAATGGTT+CGG	+	chr8.4:27622049-27622068	MS.gene28545:CDS	40.0%
	TATGACAACTGGTGTAGAGA+AGG	+	chr8.4:27622619-27622638	MS.gene28545:CDS	40.0%
	TATTGAACAACCATCAAGGG+AGG	+	chr8.4:27622586-27622605	MS.gene28545:CDS	40.0%
	TCAAGTACACAATTTACCGG+TGG	+	chr8.4:27621968-27621987	MS.gene28545:CDS	40.0%
	TGAACCCATTGAAGAAGGAA+AGG	+	chr8.4:27622540-27622559	MS.gene28545:CDS	40.0%
	TGCACTTCCATGGTGTATTA+TGG	+	chr8.4:27623244-27623263	MS.gene28545:intron	40.0%
	TGGTATGTTAGGTCATATGG+AGG	+	chr8.4:27622217-27622236	MS.gene28545:CDS	40.0%
	TGGTGTAGAGAAGGAAATAG+TGG	+	chr8.4:27622628-27622647	MS.gene28545:CDS	40.0%
	TTCATCCCCATTATCCTAAG+TGG	+	chr8.4:27623138-27623157	MS.gene28545:intron	40.0%
	TTTATCTCCACTCGGATATC+TGG	-	chr8.4:27622299-27622318	None:intergenic	40.0%
	TTTATTTGTGCCTCCCTTGA+TGG	-	chr8.4:27622599-27622618	None:intergenic	40.0%
!	CCCTCCTTCTTTCATTTTCT+GGG	-	chr8.4:27623187-27623206	None:intergenic	40.0%
!	CTTGATGGTAGAAGCAAGAA+GGG	+	chr8.4:27622671-27622690	MS.gene28545:CDS	40.0%
!	TCCCTCCTTCTTTCATTTTC+TGG	-	chr8.4:27623188-27623207	None:intergenic	40.0%
!	TTCATGATCTGGAATTGTCG+GGG	+	chr8.4:27622868-27622887	MS.gene28545:intron	40.0%
!!	AGCGGAGGATTAGCAATTTT+TGG	+	chr8.4:27623054-27623073	MS.gene28545:intron	40.0%
!!!	CATTTTTTATCGGCAAGTCC+TGG	+	chr8.4:27622818-27622837	MS.gene28545:intron	40.0%
	ACACAAGCAGAAGGACATGA+TGG	+	chr8.4:27622490-27622509	MS.gene28545:CDS	45.0%
	AGTTCCCACACTTAGAGACT+TGG	+	chr8.4:27622909-27622928	MS.gene28545:intron	45.0%
	ATGAAAGAAGGAGGGAGTCA+TGG	+	chr8.4:27623192-27623211	MS.gene28545:intron	45.0%
	CCCTAGCATAACTGATCCAT+TGG	-	chr8.4:27622468-27622487	None:intergenic	45.0%
	CGAAAACCTCTTATCCTCCA+CGG	-	chr8.4:27623326-27623345	None:intergenic	45.0%
	GAACGTTGGAAACTATGTGG+GGG	+	chr8.4:27622016-27622035	MS.gene28545:CDS	45.0%
	GATAAAATCGGAAGAAGCGG+AGG	+	chr8.4:27623039-27623058	MS.gene28545:intron	45.0%
	GCTGCAAGTTCTATCACCAA+TGG	+	chr8.4:27622449-27622468	MS.gene28545:CDS	45.0%
	TGAAAGAAGGAGGGAGTCAT+GGG	+	chr8.4:27623193-27623212	MS.gene28545:intron	45.0%
	TGAAATACCGGAGGAAGAAC+TGG	+	chr8.4:27621644-27621663	MS.gene28545:CDS	45.0%
!	ATCTGGAATTGTCGGGGTTT+GGG	+	chr8.4:27622874-27622893	MS.gene28545:intron	45.0%
!	CGATGAGTTTGAAATACCGG+AGG	+	chr8.4:27621635-27621654	MS.gene28545:CDS	45.0%
!	CGTGGAGGATAAGAGGTTTT+CGG	+	chr8.4:27623324-27623343	MS.gene28545:intron	45.0%
!	GCTTGATGGTAGAAGCAAGA+AGG	+	chr8.4:27622670-27622689	MS.gene28545:CDS	45.0%
!	GTGGAGGATAAGAGGTTTTC+GGG	+	chr8.4:27623325-27623344	MS.gene28545:intron	45.0%
!	TGCTAAGTACAGTCCCGTTT+TGG	+	chr8.4:27621943-27621962	MS.gene28545:CDS	45.0%
!!	ATCCTGGGAGTATTGACAGA+GGG	+	chr8.4:27621900-27621919	MS.gene28545:intron	45.0%
!!	CAATTTACCGGTGGGCTATA+TGG	+	chr8.4:27621977-27621996	MS.gene28545:CDS	45.0%
!!	GGGTTTTGATGCATCGTTTG+CGG	+	chr8.4:27623014-27623033	MS.gene28545:intron	45.0%
!!	TTAGGGTTATGGTGGATGTG+AGG	+	chr8.4:27622096-27622115	MS.gene28545:CDS	45.0%
	AAACCTCTTATCCTCCACGG+CGG	-	chr8.4:27623323-27623342	None:intergenic	50.0%
	CTAAGTGTGGGAACTGCACT+CGG	-	chr8.4:27622904-27622923	None:intergenic	50.0%
	GAAACGGTGGGTAAGAACGT+TGG	+	chr8.4:27622002-27622021	MS.gene28545:CDS	50.0%
	TAAGTGTGGGAACTGCACTC+GGG	-	chr8.4:27622903-27622922	None:intergenic	50.0%
	TGATAGAGCTATGGCGACTC+AGG	+	chr8.4:27623448-27623467	MS.gene28545:intron	50.0%
!	GATCTGGAATTGTCGGGGTT+TGG	+	chr8.4:27622873-27622892	MS.gene28545:intron	50.0%
!!	ACCATGATGGCGGAAGTAGA+TGG	+	chr8.4:27622327-27622346	MS.gene28545:CDS	50.0%
!!	CCATGATGGCGGAAGTAGAT+GGG	+	chr8.4:27622328-27622347	MS.gene28545:CDS	50.0%
!!	GAGGATAAGAGGTTTTCGGG+AGG	+	chr8.4:27623328-27623347	MS.gene28545:intron	50.0%
!!	GATCCTGGGAGTATTGACAG+AGG	+	chr8.4:27621899-27621918	MS.gene28545:intron	50.0%
!!	TCCTGGGAGTATTGACAGAG+GGG	+	chr8.4:27621901-27621920	MS.gene28545:intron	50.0%
!!	TTTACCGGTGGGCTATATGG+CGG	+	chr8.4:27621980-27621999	MS.gene28545:CDS	50.0%
	CCCATCTACTTCCGCCATCA+TGG	-	chr8.4:27622331-27622350	None:intergenic	55.0%
	CCTAGCACCAGTTCTTCCTC+CGG	-	chr8.4:27621654-27621673	None:intergenic	55.0%
	CGGGCCAAGTCTCTAAGTGT+GGG	-	chr8.4:27622916-27622935	None:intergenic	55.0%
	CTAGTCACTAGTGAAGGGCG+TGG	+	chr8.4:27621770-27621789	MS.gene28545:intron	55.0%
	GCTAGGGGAACACAAGCAGA+AGG	+	chr8.4:27622481-27622500	MS.gene28545:CDS	55.0%
	GGAAGTAGATGGGCGGTAGA+AGG	+	chr8.4:27622338-27622357	MS.gene28545:CDS	55.0%
	GTTTCCGCCATATAGCCCAC+CGG	-	chr8.4:27621987-27622006	None:intergenic	55.0%
	TTGGGGTCCAGATATCCGAG+TGG	+	chr8.4:27622289-27622308	MS.gene28545:CDS	55.0%
!	GAGGAAGAACTGGTGCTAGG+CGG	+	chr8.4:27621654-27621673	MS.gene28545:CDS	55.0%
!	GGCTATATGGCGGAAACGGT+GGG	+	chr8.4:27621990-27622009	MS.gene28545:CDS	55.0%
!	GTAGAGTTGATCATCCGCCG+TGG	+	chr8.4:27623306-27623325	MS.gene28545:intron	55.0%
!!	AGGTATTGAGAGGAGGGCCT+TGG	+	chr8.4:27621856-27621875	MS.gene28545:intron	55.0%
!!	AGGTTTTCGGGAGGCTGTTC+AGG	+	chr8.4:27623337-27623356	MS.gene28545:intron	55.0%
!!	CCTGGGAGTATTGACAGAGG+GGG	+	chr8.4:27621902-27621921	MS.gene28545:intron	55.0%
!!	GGTGGGCTATATGGCGGAAA+CGG	+	chr8.4:27621986-27622005	MS.gene28545:CDS	55.0%
!!	TGATGGCGGAAGTAGATGGG+CGG	+	chr8.4:27622331-27622350	MS.gene28545:CDS	55.0%
	CATCCGCCGTGGAGGATAAG+AGG	+	chr8.4:27623317-27623336	MS.gene28545:intron	60.0%
	CCCCCTCTGTCAATACTCCC+AGG	-	chr8.4:27621905-27621924	None:intergenic	60.0%
	CCGGAGGAAGAACTGGTGCT+AGG	+	chr8.4:27621651-27621670	MS.gene28545:CDS	60.0%
	CGGGAGGCTGTTCAGGATAG+TGG	+	chr8.4:27623344-27623363	MS.gene28545:intron	60.0%
	CGTCTTCCTCCCCCAATCGT+AGG	-	chr8.4:27621617-27621636	None:intergenic	60.0%
	GCGGGCCAAGTCTCTAAGTG+TGG	-	chr8.4:27622917-27622936	None:intergenic	60.0%
	GGAACACCTACGATTGGGGG+AGG	+	chr8.4:27621608-27621627	MS.gene28545:CDS	60.0%
	GGTGGATGTGAGGAAGCCGT+TGG	+	chr8.4:27622106-27622125	MS.gene28545:CDS	60.0%
!	GAACTGGTGCTAGGCGGAGT+AGG	+	chr8.4:27621660-27621679	MS.gene28545:CDS	60.0%
!	GAGTTGATCATCCGCCGTGG+AGG	+	chr8.4:27623309-27623328	MS.gene28545:intron	60.0%
!	GGGCTATATGGCGGAAACGG+TGG	+	chr8.4:27621989-27622008	MS.gene28545:CDS	60.0%
	ATCGGCAAGTCCTGGCAGCC+AGG	+	chr8.4:27622826-27622845	MS.gene28545:intron	65.0%
	CTCATTGCTCCCGGCAGTCC+TGG	-	chr8.4:27622847-27622866	None:intergenic	65.0%
	GAGCTATGGCGACTCAGGCG+TGG	+	chr8.4:27623453-27623472	MS.gene28545:intron	65.0%
	GTCCTGGCAGCCAGGACTGC+CGG	+	chr8.4:27622834-27622853	MS.gene28545:intron	70.0%
	TCCTGGCAGCCAGGACTGCC+GGG	+	chr8.4:27622835-27622854	MS.gene28545:intron	70.0%
	TCCCGGCAGTCCTGGCTGCC+AGG	-	chr8.4:27622839-27622858	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	27621606	27623507	27621606	ID=MS.gene28545
chr8.4	mRNA	27621606	27623507	27621606	ID=MS.gene28545.t1;Parent=MS.gene28545
chr8.4	exon	27621606	27621681	27621606	ID=MS.gene28545.t1.exon1;Parent=MS.gene28545.t1
chr8.4	CDS	27621606	27621681	27621606	ID=cds.MS.gene28545.t1;Parent=MS.gene28545.t1
chr8.4	exon	27621919	27622773	27621919	ID=MS.gene28545.t1.exon2;Parent=MS.gene28545.t1
chr8.4	CDS	27621919	27622773	27621919	ID=cds.MS.gene28545.t1;Parent=MS.gene28545.t1
chr8.4	exon	27623500	27623507	27623500	ID=MS.gene28545.t1.exon3;Parent=MS.gene28545.t1
chr8.4	CDS	27623500	27623507	27623500	ID=cds.MS.gene28545.t1;Parent=MS.gene28545.t1

Gene Sequence

>MS.gene28545

ATGGAACACCTACGATTGGGGGAGGAAGACGATGAGTTTGAAATACCGGAGGAAGAACTGGTGCTAGGCGGAGTAGGTTTTGATGCAAATTTGTGTCTGGTTAGGAGATTTCTGTCATATAAACCAGTCAGAACTCATATGCTAAAGGAAACTATGAAGAATATCTAGTCACTAGTGAAGGGCGTGGAAATAAGAGAAATTAAGGCGGGTGTTTTCATCTTTCAATTCTTTCATCAACGTGATATTCAAAAGGTATTGAGAGGAGGGCCTTGGTTTTTCAACAAACACATGTTGATCCTGGGAGTATTGACAGAGGGGGAAAATCCAGAACATGTCATGCTAAGTACAGTCCCGTTTTGGATTCAAGTACACAATTTACCGGTGGGCTATATGGCGGAAACGGTGGGTAAGAACGTTGGAAACTATGTGGGGGAGTTTCTTGAGTATGACGAGAAGAATGGTTCGGATTTTTGGAAACGATATATGAGAATTAGGGTTATGGTGGATGTGAGGAAGCCGTTGGTTTGCGCTAAAAGTATCAAAAAGAAAGGGGCGACACCTGTTTTAGTTAACATTAAGTATGAACGGCTAGGCATCTTTTGTTATTACTGTGGTATGTTAGGTCATATGGAGGACTCATGCGAGAAACTTTATGTCGTTGAAGATGATGATGGAATTAGAAATTGGGGTCCAGATATCCGAGTGGAGATAAATCGAAGAAACCATGATGGCGGAAGTAGATGGGCGGTAGAAGGAAGTTCTCAGTCCGATAAAAAATCAACACAAGGAGGAGGAATAACAACTAATGTTGCATATGATGCCGTAATTAATGGCATAAGTGGAGCTGCAAGTTCTATCACCAATGGATCAGTTATGCTAGGGGAACACAAGCAGAAGGACATGATGGCAGAATTAATAAAACAACAAACAAAGATGAACCCATTGAAGAAGGAAAGGATTTTCAAATATGAGCACAAAAGTATTGAACAACCATCAAGGGAGGCACAAATAAATATGACAACTGGTGTAGAGAAGGAAATAGTGGAATCTGATAAGAAAAGGATGCTTGATGGTAGAAGCAAGAAGGGTAGTGTGATAGATAATTTCGGCAATCAGAACAAAAATGAGAATCATATGCAAATTGATGGAAATACAAAACTGATGCAAAGTAAGTCAACTGATGAAGTACATGGTAAAGCAGCGCAAAATATTCATTTTTTATCGGCAAGTCCTGGCAGCCAGGACTGCCGGGAGCAATGAGTTTCATGATCTGGAATTGTCGGGGTTTGGGCAACCCGAGTGCAGTTCCCACACTTAGAGACTTGGCCCGCAAGTTTCGTTTAGATGTCCTTTTTCTTTGTGAAACAATGGTACATGCTAATAAAATTGAAGAAATTAGAGTCAGATTGGGTTTTGATGCATCGTTTGCGGTTGATAAAATCGGAAGAAGCGGAGGATTAGCAATTTTTGGAAGCACCCTTTTGATTGCAATCTTATTAATTTTTCTCAAAATTTTATTAATATGGAGGTACTTCATCCCCATTATCCTAAGTGGAGACTTACATGTTTTTATGGTTACCCAGAAAATGAAAGAAGGAGGGAGTCATGGGACTTACTTCGAACTCTATCACAAGATAATGCACTTCCATGGTGTATTATGGGAGACTTTAATGACTTGTTATCAAATGACGAAAGAAGAAGTAGAGTTGATCATCCGCCGTGGAGGATAAGAGGTTTTCGGGAGGCTGTTCAGGATAGTGGTTTGATAGATCTTCATTTGACAGGATATCCGTTTACTTTGGTCAAAGGAAGAGGAAGTCAAGATTTGAAGGAAGAAAGAATTGATAGAGCTATGGCGACTCAGGCGTGGTGTGACATTTTTCGTCAACATCAGCTCCATAA

Protein sequence

>MS.gene28545.t1

MEHLRLGEEDDEFEIPEEELVLGGVEGENPEHVMLSTVPFWIQVHNLPVGYMAETVGKNVGNYVGEFLEYDEKNGSDFWKRYMRIRVMVDVRKPLVCAKSIKKKGATPVLVNIKYERLGIFCYYCGMLGHMEDSCEKLYVVEDDDGIRNWGPDIRVEINRRNHDGGSRWAVEGSSQSDKKSTQGGGITTNVAYDAVINGISGAASSITNGSVMLGEHKQKDMMAELIKQQTKMNPLKKERIFKYEHKSIEQPSREAQINMTTGVEKEIVESDKKRMLDGRSKKGSVIDNFGNQNKNENHMQIDGNTKLMQTP