AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene042651

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042651.t1 MTR_3g053920 65.138 327 48 3 1 326 1 262 5.14e-137 392
MS.gene042651.t1 MTR_2g061500 84.496 129 20 0 1 129 1 129 2.83e-74 227
MS.gene042651.t1 MTR_4g103530 79.381 97 20 0 55 151 1 97 6.91e-51 169
MS.gene042651.t1 MTR_4g103530 49.333 75 36 2 251 323 119 193 2.60e-12 65.5
MS.gene042651.t1 MTR_7g011710 65.882 85 29 0 55 139 1 85 2.11e-33 122
MS.gene042651.t1 MTR_8g463110 66.981 106 19 1 287 376 1 106 8.69e-32 127
MS.gene042651.t1 MTR_6g033215 32.948 173 114 1 29 199 122 294 4.31e-30 117
MS.gene042651.t1 MTR_0010s0370 34.463 177 106 3 8 177 4 177 6.63e-28 108
MS.gene042651.t1 MTR_0010s0350 34.463 177 106 3 8 177 4 177 6.63e-28 108
MS.gene042651.t1 MTR_1g036720 32.110 109 74 0 4 112 2 110 8.62e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042651.t1 AT2G41590 26.761 142 100 2 77 216 80 219 2.03e-12 68.2

Find 111 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGTCCATCATACGACTTAA+AGG 0.275279 5.2:+46204860 None:intergenic
CCTATGCCTTGACCTACTTT+TGG 0.279092 5.2:+46204897 None:intergenic
TGATTGCAATGATTTGATTC+TGG 0.312324 5.2:+46204433 None:intergenic
CACCTTCAACAGTTTCATTT+CGG 0.317119 5.2:+46204493 None:intergenic
CTTTGAACAACATAGACTTT+TGG 0.318097 5.2:-46204956 MS.gene042651:CDS
ACCCTCTTGCCTGGCTTCCA+TGG 0.319573 5.2:+46205131 None:intergenic
CCGACGACGGTGTCCGCAAT+TGG 0.332786 5.2:-46204635 MS.gene042651:CDS
CCATCGATGTTAAGGCTATT+AGG 0.339423 5.2:+46205296 None:intergenic
CAGGTTCAGAAGCAAGGTTT+AGG 0.340461 5.2:+46205246 None:intergenic
CAAGGCATAGGAAGTTTCTT+AGG 0.352009 5.2:-46204885 MS.gene042651:CDS
CGGTTCCAAGTTTCAATGAT+AGG 0.362058 5.2:-46204467 MS.gene042651:CDS
TGATGTTCTTGCTGCTCAAC+TGG 0.363734 5.2:-46204406 MS.gene042651:CDS
CGACGACGGTGTCCGCAATT+GGG 0.371058 5.2:-46204634 MS.gene042651:CDS
GTGATTGTGACCCTCTTGCC+TGG 0.380233 5.2:+46205122 None:intergenic
GAGTCTGCAGCAGTAGTAAA+AGG 0.381125 5.2:+46204246 None:intergenic
TTCAAGTTCATGGCTTACCC+TGG 0.395941 5.2:-46204932 MS.gene042651:CDS
GTATGGCGCGGCACAGCAGC+TGG 0.398040 5.2:+46204540 None:intergenic
CCCTCTTGCCTGGCTTCCAT+GGG 0.400375 5.2:+46205132 None:intergenic
AGGTTCAGAAGCAAGGTTTA+GGG 0.410535 5.2:+46205247 None:intergenic
CTGGTGCTTGGACTGCCTGC+TGG 0.413675 5.2:-46204276 MS.gene042651:CDS
TAACTTTAAATATGAGAAGC+TGG 0.420500 5.2:-46204730 MS.gene042651:CDS
GTCCTTGAACACTGTCTGAT+TGG 0.428037 5.2:-46205212 MS.gene042651:CDS
TCTACTCCGCCTCTGGTGCT+TGG 0.429714 5.2:-46204288 MS.gene042651:CDS
GTCAAAGCAAAACAAGCTGC+TGG 0.437669 5.2:-46204381 MS.gene042651:intron
CCTGCTTTCTACTCCGCCTC+TGG 0.438931 5.2:-46204295 MS.gene042651:CDS
TCAAGTTCATGGCTTACCCT+GGG 0.443119 5.2:-46204931 MS.gene042651:CDS
GGGTAAGGCCATCGATGTTA+AGG 0.453736 5.2:+46205288 None:intergenic
GGGTTAAACCCTCCTCAGAC+AGG 0.460583 5.2:+46205267 None:intergenic
TCAAGGACAGCAGCGGTGTC+AGG 0.460723 5.2:+46205227 None:intergenic
GACCAATCAGACAGTGTTCA+AGG 0.479711 5.2:+46205210 None:intergenic
GGTACCTTTAAGTCGTATGA+TGG 0.483619 5.2:-46204864 MS.gene042651:CDS
CACGGGCTGCATCAACTTTG+TGG 0.484205 5.2:+46205063 None:intergenic
CGCGGCACAGCAGCTGGGAT+AGG 0.489826 5.2:+46204546 None:intergenic
AACCCTCCTCAGACAGGGTA+AGG 0.493201 5.2:+46205273 None:intergenic
AAAAGTCTATGTTGTTCAAA+GGG 0.497079 5.2:+46204958 None:intergenic
TGTCACAGGTGTGGATTGCT+CGG 0.497819 5.2:-46204699 MS.gene042651:CDS
TGCAACAAATAGGTGGATAC+AGG 0.498808 5.2:-46204571 MS.gene042651:CDS
GTGTGGATTGCTCGGACATA+CGG 0.498881 5.2:-46204691 MS.gene042651:CDS
CAAAAGTCTATGTTGTTCAA+AGG 0.500229 5.2:+46204957 None:intergenic
GTAAAAGGAAACGGACCAGC+AGG 0.501080 5.2:+46204261 None:intergenic
TTGGTACTGCTGCAACAAAT+AGG 0.502007 5.2:-46204581 MS.gene042651:CDS
ATGTCTCGCCCATGGAAGCC+AGG 0.503486 5.2:-46205140 MS.gene042651:CDS
CTATCATTGAAACTTGGAAC+CGG 0.511071 5.2:+46204468 None:intergenic
GCAGACTCGCAAGAGGAAAC+CGG 0.514022 5.2:-46204231 MS.gene042651:CDS
ACATACGGACAAGGTGTGTC+CGG 0.515722 5.2:-46204676 MS.gene042651:CDS
GTACCAACACGTTGCAAAGC+AGG 0.516840 5.2:+46204597 None:intergenic
GTCATGGATCAGATTTCCCT+TGG 0.522464 5.2:-46204999 MS.gene042651:CDS
TGAAACTGTTGAAGGTGCTC+CGG 0.523051 5.2:-46204487 MS.gene042651:CDS
ATGAATTGTCACCATTCTGA+AGG 0.523788 5.2:+46204175 None:intergenic
ACCATCCTATCATTGAAACT+TGG 0.524831 5.2:+46204462 None:intergenic
ACACGCCACTCTTGTTTCAA+AGG 0.525117 5.2:+46204783 None:intergenic
AAGCAGCAACAACATTTGTA+TGG 0.530163 5.2:+46204523 None:intergenic
AGCCGAAATGAAACTGTTGA+AGG 0.531447 5.2:-46204495 MS.gene042651:CDS
GGCCTTACCCTGTCTGAGGA+GGG 0.534585 5.2:-46205275 MS.gene042651:CDS
AGACATACGCTCGCTGAAGT+AGG 0.537827 5.2:+46205157 None:intergenic
TGCTACGCGTATGCACATAT+AGG 0.538576 5.2:+46204821 None:intergenic
GCAGGCAGTCCAAGCACCAG+AGG 0.539792 5.2:+46204279 None:intergenic
CTGACTTGAAGAAAAGAAAG+AGG 0.541539 5.2:-46204206 MS.gene042651:CDS
GGCAGTCCAAGCACCAGAGG+CGG 0.547639 5.2:+46204282 None:intergenic
AACTTTAAATATGAGAAGCT+GGG 0.549150 5.2:-46204729 MS.gene042651:CDS
GACGACGGTGTCCGCAATTG+GGG 0.552015 5.2:-46204633 MS.gene042651:CDS
GTTAAGGCTATTAGGATCCA+TGG 0.554062 5.2:+46205304 None:intergenic
GCTTCCAACTCAAACAGCTC+CGG 0.554973 5.2:+46204657 None:intergenic
TGGCCTTACCCTGTCTGAGG+AGG 0.556700 5.2:-46205276 MS.gene042651:CDS
CCAGAGGCGGAGTAGAAAGC+AGG 0.563408 5.2:+46204295 None:intergenic
TATGGCGCGGCACAGCAGCT+GGG 0.566514 5.2:+46204541 None:intergenic
TCCAAGTTTCAATGATAGGA+TGG 0.567934 5.2:-46204463 MS.gene042651:CDS
CGGTGTCAGGTTCAGAAGCA+AGG 0.568155 5.2:+46205240 None:intergenic
TTTGCTGCTGATGGGGATGA+GGG 0.573290 5.2:-46204150 MS.gene042651:intron
ATAACGGTCAGCCACAGACT+TGG 0.574286 5.2:+46205100 None:intergenic
GTTGTTCAAAGGGACAAAAG+TGG 0.574370 5.2:+46204968 None:intergenic
GTGTCCGGAGCTGTTTGAGT+TGG 0.576270 5.2:-46204661 MS.gene042651:CDS
CAAAAGTACCATTGCTAGAG+CGG 0.577625 5.2:+46204760 None:intergenic
GCAGCAGTAGTAAAAGGAAA+CGG 0.578310 5.2:+46204252 None:intergenic
AAGCCTGCTTTGCAACGTGT+TGG 0.578917 5.2:-46204600 MS.gene042651:CDS
ATCCAACCAAAAGTAGGTCA+AGG 0.581878 5.2:-46204903 MS.gene042651:CDS
TGGCGTGTCCGCTCTAGCAA+TGG 0.593204 5.2:-46204768 MS.gene042651:CDS
GCAGCCCGTGTTCTTGATGA+AGG 0.594196 5.2:-46205050 MS.gene042651:CDS
CAAGTTCATGGCTTACCCTG+GGG 0.594408 5.2:-46204930 MS.gene042651:CDS
GTATGCACATATAGGAGCTA+TGG 0.594752 5.2:+46204829 None:intergenic
CCTAATAGCCTTAACATCGA+TGG 0.595474 5.2:-46205296 MS.gene042651:CDS
GGTGGAACTGAAAGAGATAA+CGG 0.597519 5.2:+46205084 None:intergenic
TGAGATAAGCACCCCAATTG+CGG 0.598005 5.2:+46204622 None:intergenic
TACTGCTGCAGACTCGCAAG+AGG 0.598689 5.2:-46204238 MS.gene042651:CDS
GTGTTCTTGATGAAGGTCTG+TGG 0.601022 5.2:-46205043 MS.gene042651:CDS
CCCATGGAAGCCAGGCAAGA+GGG 0.601100 5.2:-46205132 MS.gene042651:CDS
AAAAGTGGGAACGACACCAA+GGG 0.602228 5.2:+46204983 None:intergenic
GGATTTATCCAACCAAAAGT+AGG 0.604485 5.2:-46204909 MS.gene042651:CDS
TTGTTCAAAGGGACAAAAGT+GGG 0.605801 5.2:+46204969 None:intergenic
CCAATTGCGGACACCGTCGT+CGG 0.608993 5.2:+46204635 None:intergenic
AGGATGCTGACCCTTCAGAA+TGG 0.609247 5.2:-46204186 MS.gene042651:CDS
AGGCGGAGTAGAAAGCAGGT+TGG 0.609570 5.2:+46204299 None:intergenic
GCCCATGGAAGCCAGGCAAG+AGG 0.610006 5.2:-46205133 MS.gene042651:CDS
GCGAGCGTATGTCTCGCCCA+TGG 0.615949 5.2:-46205148 MS.gene042651:CDS
TTGTTCCTTTGAAACAAGAG+TGG 0.618213 5.2:-46204788 MS.gene042651:CDS
CCAAAAGTAGGTCAAGGCAT+AGG 0.620618 5.2:-46204897 MS.gene042651:CDS
ATTGAATCTCGCGATCAGCA+AGG 0.621915 5.2:+46205183 None:intergenic
CGATGGCCTTACCCTGTCTG+AGG 0.624862 5.2:-46205279 MS.gene042651:CDS
GGGCTGCATCAACTTTGTGG+TGG 0.630504 5.2:+46205066 None:intergenic
TGAATTGTCACCATTCTGAA+GGG 0.634296 5.2:+46204176 None:intergenic
GGTCACAATCACCAAGTCTG+TGG 0.636667 5.2:-46205111 MS.gene042651:CDS
GTACTGCTGCAACAAATAGG+TGG 0.637449 5.2:-46204578 MS.gene042651:CDS
GCAACAACATTTGTATGGCG+CGG 0.643692 5.2:+46204528 None:intergenic
TTTGGTTGGATAAATCCCCA+GGG 0.649861 5.2:+46204915 None:intergenic
TTTGAGTTGGAAGCCGACGA+CGG 0.652242 5.2:-46204648 MS.gene042651:CDS
CAAAAGTGGGAACGACACCA+AGG 0.656940 5.2:+46204982 None:intergenic
GGTTAAACCCTCCTCAGACA+GGG 0.659380 5.2:+46205268 None:intergenic
ACAGACCTTCATCAAGAACA+CGG 0.665987 5.2:+46205045 None:intergenic
CAGACCTTCATCAAGAACAC+GGG 0.677016 5.2:+46205046 None:intergenic
ATTGCTCGGACATACGGACA+AGG 0.677946 5.2:-46204685 MS.gene042651:CDS
ACAGTGTTCAAGGACAGCAG+CGG 0.718554 5.2:+46205220 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAAGTCTATGTTGTTCAAA+GGG + chr5.2:46203634-46203653 None:intergenic 25.0%
! AACTTTAAATATGAGAAGCT+GGG - chr5.2:46203860-46203879 MS.gene042651:intron 25.0%
! ATAGGAACAAATGTTAATCA+TGG - chr5.2:46204472-46204491 MS.gene042651:CDS 25.0%
! CAACTTTCAAATCAATTTAC+AGG - chr5.2:46204951-46204970 MS.gene042651:CDS 25.0%
! TAACTTTAAATATGAGAAGC+TGG - chr5.2:46203859-46203878 MS.gene042651:intron 25.0%
! TAGGAACAAATGTTAATCAT+GGG - chr5.2:46204473-46204492 MS.gene042651:CDS 25.0%
!! TGATAATTTTCATATCGTCA+TGG - chr5.2:46203574-46203593 MS.gene042651:intron 25.0%
!! TTTGAACAACATAGACTTTT+GGG - chr5.2:46203634-46203653 MS.gene042651:intron 25.0%
!!! GAAATTTTTGATAGTGTTCT+TGG - chr5.2:46204855-46204874 MS.gene042651:CDS 25.0%
AAAGTTTCAACATAAGCAGT+TGG + chr5.2:46205215-46205234 None:intergenic 30.0%
AACTGCTTATGTTGAAACTT+TGG - chr5.2:46205214-46205233 MS.gene042651:CDS 30.0%
AGGAACAAATGTTAATCATG+GGG - chr5.2:46204474-46204493 MS.gene042651:CDS 30.0%
CAAAAGTCTATGTTGTTCAA+AGG + chr5.2:46203635-46203654 None:intergenic 30.0%
GTTTGATGAAACTGATGTAA+TGG - chr5.2:46204531-46204550 MS.gene042651:CDS 30.0%
TCACTTAGGAATAAGATATC+CGG + chr5.2:46204672-46204691 None:intergenic 30.0%
TGATTGCAATGATTTGATTC+TGG + chr5.2:46204159-46204178 None:intergenic 30.0%
! CTTTGAACAACATAGACTTT+TGG - chr5.2:46203633-46203652 MS.gene042651:intron 30.0%
AAAAAGCTGTTCTTGACTCT+GGG - chr5.2:46205069-46205088 MS.gene042651:CDS 35.0%
AAAATGGCGCTTAGCAATAT+AGG + chr5.2:46205297-46205316 None:intergenic 35.0%
AAATGGCGCTTAGCAATATA+GGG + chr5.2:46205296-46205315 None:intergenic 35.0%
AACAAATGTTAATCATGGGG+GGG - chr5.2:46204477-46204496 MS.gene042651:CDS 35.0%
AAGCAGCAACAACATTTGTA+TGG + chr5.2:46204069-46204088 None:intergenic 35.0%
ACATCAGTTTCATCAAACAG+TGG + chr5.2:46204529-46204548 None:intergenic 35.0%
ACCATCCTATCATTGAAACT+TGG + chr5.2:46204130-46204149 None:intergenic 35.0%
ATGAATTGTCACCATTCTGA+AGG + chr5.2:46204417-46204436 None:intergenic 35.0%
CACCTTCAACAGTTTCATTT+CGG + chr5.2:46204099-46204118 None:intergenic 35.0%
CATCAGTTTCATCAAACAGT+GGG + chr5.2:46204528-46204547 None:intergenic 35.0%
CTATCATTGAAACTTGGAAC+CGG + chr5.2:46204124-46204143 None:intergenic 35.0%
GAACAAATGTTAATCATGGG+GGG - chr5.2:46204476-46204495 MS.gene042651:CDS 35.0%
GAATTACCTGTTGAAAAGGA+CGG + chr5.2:46204265-46204284 None:intergenic 35.0%
GATGAGGGTAACAATGTAAT+AGG - chr5.2:46204454-46204473 MS.gene042651:CDS 35.0%
GGAACAAATGTTAATCATGG+GGG - chr5.2:46204475-46204494 MS.gene042651:CDS 35.0%
GGAGCAAGGTTAACATAAAT+AGG + chr5.2:46205263-46205282 None:intergenic 35.0%
GGATTTATCCAACCAAAAGT+AGG - chr5.2:46203680-46203699 MS.gene042651:intron 35.0%
GGTTACTATGGTTATCCTAA+TGG - chr5.2:46204894-46204913 MS.gene042651:CDS 35.0%
TACTATGGTTATCCTAATGG+TGG - chr5.2:46204897-46204916 MS.gene042651:CDS 35.0%
TCCAAGTTTCAATGATAGGA+TGG - chr5.2:46204126-46204145 MS.gene042651:intron 35.0%
TGAATTGTCACCATTCTGAA+GGG + chr5.2:46204416-46204435 None:intergenic 35.0%
TTAAGGTCAGGAATTGTACT+CGG + chr5.2:46204627-46204646 None:intergenic 35.0%
TTATCCAATGTTCCGATTGA+AGG - chr5.2:46205092-46205111 MS.gene042651:CDS 35.0%
TTCACATGGTTCAAAAGTCT+TGG - chr5.2:46205122-46205141 MS.gene042651:CDS 35.0%
TTGTCGTAAAAAGTTCCAAG+CGG + chr5.2:46204934-46204953 None:intergenic 35.0%
TTGTTCAAAGGGACAAAAGT+GGG + chr5.2:46203623-46203642 None:intergenic 35.0%
! AATTCATCTTTTGCTGCTGA+TGG - chr5.2:46204430-46204449 MS.gene042651:CDS 35.0%
! AGACTTTTGAACCATGTGAA+AGG + chr5.2:46205122-46205141 None:intergenic 35.0%
! ATTCATCTTTTGCTGCTGAT+GGG - chr5.2:46204431-46204450 MS.gene042651:CDS 35.0%
! CCTTTTCAACAGGTAATTCA+GGG - chr5.2:46204266-46204285 MS.gene042651:CDS 35.0%
! CTGACTTGAAGAAAAGAAAG+AGG - chr5.2:46204383-46204402 MS.gene042651:CDS 35.0%
! GACTTTTGAACCATGTGAAA+GGG + chr5.2:46205121-46205140 None:intergenic 35.0%
! TCCTTTTCAACAGGTAATTC+AGG - chr5.2:46204265-46204284 MS.gene042651:CDS 35.0%
! TCTTTTCTTCAAGTCAGAAC+CGG + chr5.2:46204380-46204399 None:intergenic 35.0%
!! TTGTTCCTTTGAAACAAGAG+TGG - chr5.2:46203801-46203820 MS.gene042651:intron 35.0%
!!! GAAGAACTTCGTTTTTTGCT+TGG - chr5.2:46204717-46204736 MS.gene042651:CDS 35.0%
ACAGACCTTCATCAAGAACA+CGG + chr5.2:46203547-46203566 None:intergenic 40.0%
ACGTCCATCATACGACTTAA+AGG + chr5.2:46203732-46203751 None:intergenic 40.0%
AGCCGAAATGAAACTGTTGA+AGG - chr5.2:46204094-46204113 MS.gene042651:intron 40.0%
AGGTTCAGAAGCAAGGTTTA+GGG + chr5.2:46203345-46203364 None:intergenic 40.0%
ATAGGATAGTGATCAGAAGC+AGG + chr5.2:46205245-46205264 None:intergenic 40.0%
ATCCAACCAAAAGTAGGTCA+AGG - chr5.2:46203686-46203705 MS.gene042651:intron 40.0%
CAAAAAGCTGTTCTTGACTC+TGG - chr5.2:46205068-46205087 MS.gene042651:CDS 40.0%
CAAATCAATTTACAGGCCCT+TGG - chr5.2:46204958-46204977 MS.gene042651:CDS 40.0%
CAAGGCATAGGAAGTTTCTT+AGG - chr5.2:46203704-46203723 MS.gene042651:intron 40.0%
CCATCGATGTTAAGGCTATT+AGG + chr5.2:46203296-46203315 None:intergenic 40.0%
CCCTGAATTACCTGTTGAAA+AGG + chr5.2:46204269-46204288 None:intergenic 40.0%
CGGTTCCAAGTTTCAATGAT+AGG - chr5.2:46204122-46204141 MS.gene042651:intron 40.0%
CTAAACTCATTTACCTAGGC+AGG + chr5.2:46204575-46204594 None:intergenic 40.0%
CTGACCTTAAGTACCTAATC+CGG - chr5.2:46204637-46204656 MS.gene042651:CDS 40.0%
GATGAAACTGATGTAATGGC+AGG - chr5.2:46204535-46204554 MS.gene042651:CDS 40.0%
GCAGCAGTAGTAAAAGGAAA+CGG + chr5.2:46204340-46204359 None:intergenic 40.0%
GCCACTAAACTCATTTACCT+AGG + chr5.2:46204579-46204598 None:intergenic 40.0%
GCCTAGGTAAATGAGTTTAG+TGG - chr5.2:46204575-46204594 MS.gene042651:CDS 40.0%
GGTAAATGAGTTTAGTGGCT+TGG - chr5.2:46204580-46204599 MS.gene042651:CDS 40.0%
GGTACCTTTAAGTCGTATGA+TGG - chr5.2:46203725-46203744 MS.gene042651:intron 40.0%
GGTGGAACTGAAAGAGATAA+CGG + chr5.2:46203508-46203527 None:intergenic 40.0%
GTAACCTTCAATCGGAACAT+TGG + chr5.2:46205099-46205118 None:intergenic 40.0%
GTATGCACATATAGGAGCTA+TGG + chr5.2:46203763-46203782 None:intergenic 40.0%
GTGAAAGGGTAACCTTCAAT+CGG + chr5.2:46205107-46205126 None:intergenic 40.0%
GTTGTTCAAAGGGACAAAAG+TGG + chr5.2:46203624-46203643 None:intergenic 40.0%
TAAGGTCAGGAATTGTACTC+GGG + chr5.2:46204626-46204645 None:intergenic 40.0%
TCTTGATGCGAGTGAGAAAA+GGG - chr5.2:46205007-46205026 MS.gene042651:CDS 40.0%
TGCAACAAATAGGTGGATAC+AGG - chr5.2:46204018-46204037 MS.gene042651:intron 40.0%
TTCTTGATGCGAGTGAGAAA+AGG - chr5.2:46205006-46205025 MS.gene042651:CDS 40.0%
TTGAAGGTTACCCTTTCACA+TGG - chr5.2:46205108-46205127 MS.gene042651:CDS 40.0%
TTGGTACTGCTGCAACAAAT+AGG - chr5.2:46204008-46204027 MS.gene042651:intron 40.0%
TTTGGTTGGATAAATCCCCA+GGG + chr5.2:46203677-46203696 None:intergenic 40.0%
! CAGAGTCAAGAACAGCTTTT+TGG + chr5.2:46205070-46205089 None:intergenic 40.0%
! CCTAATAGCCTTAACATCGA+TGG - chr5.2:46203293-46203312 MS.gene042651:intron 40.0%
! CTATTACCGTCCTTTTCAAC+AGG - chr5.2:46204256-46204275 MS.gene042651:CDS 40.0%
! GTTTAGTGGCTTGGAATTGT+CGG - chr5.2:46204589-46204608 MS.gene042651:CDS 40.0%
! TGGAGACTTACTGGTTACTA+TGG - chr5.2:46204882-46204901 MS.gene042651:CDS 40.0%
! TTCATCTTTTGCTGCTGATG+GGG - chr5.2:46204432-46204451 MS.gene042651:CDS 40.0%
! TTTAGTGGCTTGGAATTGTC+GGG - chr5.2:46204590-46204609 MS.gene042651:CDS 40.0%
!! CAAAAGTACCATTGCTAGAG+CGG + chr5.2:46203832-46203851 None:intergenic 40.0%
!! TTGATAGTGTTCTTGGCTCT+TGG - chr5.2:46204862-46204881 MS.gene042651:CDS 40.0%
!! TTTTGGTTGGATAAATCCCC+AGG + chr5.2:46203678-46203697 None:intergenic 40.0%
!!! GACTTTTGGGTTCAAGTTCA+TGG - chr5.2:46203647-46203666 MS.gene042651:intron 40.0%
AAAAGTGGGAACGACACCAA+GGG + chr5.2:46203609-46203628 None:intergenic 45.0%
AAATCGCCGAAAATGCACCA+AGG + chr5.2:46204978-46204997 None:intergenic 45.0%
AATCGCCGAAAATGCACCAA+GGG + chr5.2:46204977-46204996 None:intergenic 45.0%
AGATATCCGGATTGAAGTGC+CGG + chr5.2:46204659-46204678 None:intergenic 45.0%
AGGACGGTAATAGATGTACC+AGG + chr5.2:46204249-46204268 None:intergenic 45.0%
ATTGAATCTCGCGATCAGCA+AGG + chr5.2:46203409-46203428 None:intergenic 45.0%
CAGACCTTCATCAAGAACAC+GGG + chr5.2:46203546-46203565 None:intergenic 45.0%
CAGGTTCAGAAGCAAGGTTT+AGG + chr5.2:46203346-46203365 None:intergenic 45.0%
CCAAAAGTAGGTCAAGGCAT+AGG - chr5.2:46203692-46203711 MS.gene042651:intron 45.0%
CTTGATGCGAGTGAGAAAAG+GGG - chr5.2:46205008-46205027 MS.gene042651:CDS 45.0%
GACCAATCAGACAGTGTTCA+AGG + chr5.2:46203382-46203401 None:intergenic 45.0%
GAGTCTGCAGCAGTAGTAAA+AGG + chr5.2:46204346-46204365 None:intergenic 45.0%
GCAACAACATTTGTATGGCG+CGG + chr5.2:46204064-46204083 None:intergenic 45.0%
GCATTTCTACGACCACCATT+AGG + chr5.2:46204912-46204931 None:intergenic 45.0%
GCGATCTAACCTTTCTTCGA+CGG + chr5.2:46205159-46205178 None:intergenic 45.0%
GTACTGCTGCAACAAATAGG+TGG - chr5.2:46204011-46204030 MS.gene042651:intron 45.0%
GTCAAAGCAAAACAAGCTGC+TGG - chr5.2:46204208-46204227 MS.gene042651:CDS 45.0%
GTCATGGATCAGATTTCCCT+TGG - chr5.2:46203590-46203609 MS.gene042651:intron 45.0%
GTCCTTGAACACTGTCTGAT+TGG - chr5.2:46203377-46203396 MS.gene042651:intron 45.0%
GTGTTCTTGATGAAGGTCTG+TGG - chr5.2:46203546-46203565 MS.gene042651:intron 45.0%
TCAAGTTCATGGCTTACCCT+GGG - chr5.2:46203658-46203677 MS.gene042651:intron 45.0%
TGAGATAAGCACCCCAATTG+CGG + chr5.2:46203970-46203989 None:intergenic 45.0%
TGATGTTCTTGCTGCTCAAC+TGG - chr5.2:46204183-46204202 MS.gene042651:CDS 45.0%
TGCTACGCGTATGCACATAT+AGG + chr5.2:46203771-46203790 None:intergenic 45.0%
TGTGAGCTAGAGTCTCACTT+AGG + chr5.2:46204686-46204705 None:intergenic 45.0%
TTGATGCGAGTGAGAAAAGG+GGG - chr5.2:46205009-46205028 MS.gene042651:CDS 45.0%
! AAATCGTTGATGAGCCATGG+AGG + chr5.2:46205047-46205066 None:intergenic 45.0%
! ACACGCCACTCTTGTTTCAA+AGG + chr5.2:46203809-46203828 None:intergenic 45.0%
! AGTGCCGGATTAGGTACTTA+AGG + chr5.2:46204644-46204663 None:intergenic 45.0%
! CCTATGCCTTGACCTACTTT+TGG + chr5.2:46203695-46203714 None:intergenic 45.0%
! CGGATTAGGTACTTAAGGTC+AGG + chr5.2:46204639-46204658 None:intergenic 45.0%
! TGGAAATCGTTGATGAGCCA+TGG + chr5.2:46205050-46205069 None:intergenic 45.0%
! TTAGTGGCTTGGAATTGTCG+GGG - chr5.2:46204591-46204610 MS.gene042651:CDS 45.0%
! TTCAAGTTCATGGCTTACCC+TGG - chr5.2:46203657-46203676 MS.gene042651:intron 45.0%
!! TGAAACTGTTGAAGGTGCTC+CGG - chr5.2:46204102-46204121 MS.gene042651:intron 45.0%
!! TGCCTTGACCTACTTTTGGT+TGG + chr5.2:46203691-46203710 None:intergenic 45.0%
!!! AGCTGGGTGTTTTTTGTCAC+AGG - chr5.2:46203876-46203895 MS.gene042651:intron 45.0%
!!! GGTGTTTTTTGTCACAGGTG+TGG - chr5.2:46203881-46203900 MS.gene042651:intron 45.0%
AAGCCTGCTTTGCAACGTGT+TGG - chr5.2:46203989-46204008 MS.gene042651:intron 50.0%
AATGGCAGGACCTGATGATC+AGG - chr5.2:46204549-46204568 MS.gene042651:CDS 50.0%
ACAGTGTTCAAGGACAGCAG+CGG + chr5.2:46203372-46203391 None:intergenic 50.0%
ACATACGGACAAGGTGTGTC+CGG - chr5.2:46203913-46203932 MS.gene042651:intron 50.0%
AGACATACGCTCGCTGAAGT+AGG + chr5.2:46203435-46203454 None:intergenic 50.0%
AGGATGCTGACCCTTCAGAA+TGG - chr5.2:46204403-46204422 MS.gene042651:CDS 50.0%
ATAACGGTCAGCCACAGACT+TGG + chr5.2:46203492-46203511 None:intergenic 50.0%
ATTGCTCGGACATACGGACA+AGG - chr5.2:46203904-46203923 MS.gene042651:intron 50.0%
CAAAAGTGGGAACGACACCA+AGG + chr5.2:46203610-46203629 None:intergenic 50.0%
CAAGTTCATGGCTTACCCTG+GGG - chr5.2:46203659-46203678 MS.gene042651:intron 50.0%
CCTAATCCGGCACTTCAATC+CGG - chr5.2:46204650-46204669 MS.gene042651:CDS 50.0%
GCGCACTCGCTAATAGTTTG+TGG - chr5.2:46205177-46205196 MS.gene042651:CDS 50.0%
GCTTCCAACTCAAACAGCTC+CGG + chr5.2:46203935-46203954 None:intergenic 50.0%
GGGTAAGGCCATCGATGTTA+AGG + chr5.2:46203304-46203323 None:intergenic 50.0%
GGTAATAGATGTACCAGGCC+AGG + chr5.2:46204244-46204263 None:intergenic 50.0%
GGTCACAATCACCAAGTCTG+TGG - chr5.2:46203478-46203497 MS.gene042651:intron 50.0%
GGTTAAACCCTCCTCAGACA+GGG + chr5.2:46203324-46203343 None:intergenic 50.0%
GTAAAAGGAAACGGACCAGC+AGG + chr5.2:46204331-46204350 None:intergenic 50.0%
GTACCAACACGTTGCAAAGC+AGG + chr5.2:46203995-46204014 None:intergenic 50.0%
TTAACCTTGCTCCCACACCT+CGG - chr5.2:46205270-46205289 MS.gene042651:CDS 50.0%
TTTGCTGCTGATGGGGATGA+GGG - chr5.2:46204439-46204458 MS.gene042651:CDS 50.0%
! CTTGGCTCTTGGAGACTTAC+TGG - chr5.2:46204873-46204892 MS.gene042651:CDS 50.0%
! GCGGGGGTCTTGCATTATTT+TGG - chr5.2:46204778-46204797 MS.gene042651:CDS 50.0%
! GTGTGGATTGCTCGGACATA+CGG - chr5.2:46203898-46203917 MS.gene042651:intron 50.0%
! TGTCACAGGTGTGGATTGCT+CGG - chr5.2:46203890-46203909 MS.gene042651:intron 50.0%
! TGTTTTCCTGTAGACCGCAC+CGG - chr5.2:46204750-46204769 MS.gene042651:CDS 50.0%
! TTTTGCTGCTGATGGGGATG+AGG - chr5.2:46204438-46204457 MS.gene042651:CDS 50.0%
!! ACAGGCCCTTGGTGCATTTT+CGG - chr5.2:46204969-46204988 MS.gene042651:CDS 50.0%
!! TTTGAGTTGGAAGCCGACGA+CGG - chr5.2:46203941-46203960 MS.gene042651:intron 50.0%
AACCCTCCTCAGACAGGGTA+AGG + chr5.2:46203319-46203338 None:intergenic 55.0%
AACCTTTCTTCGACGGCACG+TGG + chr5.2:46205152-46205171 None:intergenic 55.0%
ACCTTTCTTCGACGGCACGT+GGG + chr5.2:46205151-46205170 None:intergenic 55.0%
AGCAATATAGGGCCGAGGTG+TGG + chr5.2:46205285-46205304 None:intergenic 55.0%
AGGCGGAGTAGAAAGCAGGT+TGG + chr5.2:46204293-46204312 None:intergenic 55.0%
CGCTTAGCAATATAGGGCCG+AGG + chr5.2:46205290-46205309 None:intergenic 55.0%
GATGAGCCATGGAGGTCTAG+TGG + chr5.2:46205039-46205058 None:intergenic 55.0%
GCAATATAGGGCCGAGGTGT+GGG + chr5.2:46205284-46205303 None:intergenic 55.0%
GCAGACTCGCAAGAGGAAAC+CGG - chr5.2:46204358-46204377 MS.gene042651:intron 55.0%
GCAGCCCGTGTTCTTGATGA+AGG - chr5.2:46203539-46203558 MS.gene042651:intron 55.0%
GCTTGGAATTGTCGGGGTGT+AGG - chr5.2:46204597-46204616 MS.gene042651:CDS 55.0%
GGGCTGCATCAACTTTGTGG+TGG + chr5.2:46203526-46203545 None:intergenic 55.0%
GGGTTAAACCCTCCTCAGAC+AGG + chr5.2:46203325-46203344 None:intergenic 55.0%
GTGATTGTGACCCTCTTGCC+TGG + chr5.2:46203470-46203489 None:intergenic 55.0%
GTGGTCGTAGAAATGCCGCT+TGG - chr5.2:46204916-46204935 MS.gene042651:CDS 55.0%
GTGTCCGGAGCTGTTTGAGT+TGG - chr5.2:46203928-46203947 MS.gene042651:intron 55.0%
TACTGCTGCAGACTCGCAAG+AGG - chr5.2:46204351-46204370 MS.gene042651:intron 55.0%
! CACGGGCTGCATCAACTTTG+TGG + chr5.2:46203529-46203548 None:intergenic 55.0%
! CGGTGTCAGGTTCAGAAGCA+AGG + chr5.2:46203352-46203371 None:intergenic 55.0%
!! CCGGATTGAAGTGCCGGATT+AGG + chr5.2:46204653-46204672 None:intergenic 55.0%
ACCCTCTTGCCTGGCTTCCA+TGG + chr5.2:46203461-46203480 None:intergenic 60.0%
ATGTCTCGCCCATGGAAGCC+AGG - chr5.2:46203449-46203468 MS.gene042651:intron 60.0%
CCAATTGCGGACACCGTCGT+CGG + chr5.2:46203957-46203976 None:intergenic 60.0%
CCAGAGGCGGAGTAGAAAGC+AGG + chr5.2:46204297-46204316 None:intergenic 60.0%
CCCATGGAAGCCAGGCAAGA+GGG - chr5.2:46203457-46203476 MS.gene042651:intron 60.0%
CCCTCTTGCCTGGCTTCCAT+GGG + chr5.2:46203460-46203479 None:intergenic 60.0%
CCTAGGCAGGCCTGATCATC+AGG + chr5.2:46204562-46204581 None:intergenic 60.0%
CCTGATGATCAGGCCTGCCT+AGG - chr5.2:46204559-46204578 MS.gene042651:CDS 60.0%
CCTGCTTTCTACTCCGCCTC+TGG - chr5.2:46204294-46204313 MS.gene042651:CDS 60.0%
CCTTTCTTCGACGGCACGTG+GGG + chr5.2:46205150-46205169 None:intergenic 60.0%
CGACGACGGTGTCCGCAATT+GGG - chr5.2:46203955-46203974 MS.gene042651:intron 60.0%
GACGACGGTGTCCGCAATTG+GGG - chr5.2:46203956-46203975 MS.gene042651:intron 60.0%
GGCCTTACCCTGTCTGAGGA+GGG - chr5.2:46203314-46203333 MS.gene042651:intron 60.0%
TCAAGGACAGCAGCGGTGTC+AGG + chr5.2:46203365-46203384 None:intergenic 60.0%
TCTACTCCGCCTCTGGTGCT+TGG - chr5.2:46204301-46204320 MS.gene042651:CDS 60.0%
TGGCGTGTCCGCTCTAGCAA+TGG - chr5.2:46203821-46203840 MS.gene042651:intron 60.0%
! CGATGGCCTTACCCTGTCTG+AGG - chr5.2:46203310-46203329 MS.gene042651:intron 60.0%
! TGGCCTTACCCTGTCTGAGG+AGG - chr5.2:46203313-46203332 MS.gene042651:intron 60.0%
CCCCACGTGCCGTCGAAGAA+AGG - chr5.2:46205147-46205166 MS.gene042651:CDS 65.0%
CCGACGACGGTGTCCGCAAT+TGG - chr5.2:46203954-46203973 MS.gene042651:intron 65.0%
CCTCTACCGGTGCGGTCTAC+AGG + chr5.2:46204759-46204778 None:intergenic 65.0%
CTGGTGCTTGGACTGCCTGC+TGG - chr5.2:46204313-46204332 MS.gene042651:CDS 65.0%
GCAGGCAGTCCAAGCACCAG+AGG + chr5.2:46204313-46204332 None:intergenic 65.0%
GCCCATGGAAGCCAGGCAAG+AGG - chr5.2:46203456-46203475 MS.gene042651:intron 65.0%
GCGAGCGTATGTCTCGCCCA+TGG - chr5.2:46203441-46203460 MS.gene042651:intron 65.0%
GCTGCTGGTGCGACTTCTCC+TGG - chr5.2:46204223-46204242 MS.gene042651:CDS 65.0%
GGCAGTCCAAGCACCAGAGG+CGG + chr5.2:46204310-46204329 None:intergenic 65.0%
GGCGCACCACTAGACCTCCA+TGG - chr5.2:46205030-46205049 MS.gene042651:CDS 65.0%
TATGGCGCGGCACAGCAGCT+GGG + chr5.2:46204051-46204070 None:intergenic 65.0%
TGCAAGACCCCCGCCTCTAC+CGG + chr5.2:46204772-46204791 None:intergenic 65.0%
TGGTGCGACTTCTCCTGGCC+TGG - chr5.2:46204228-46204247 MS.gene042651:CDS 65.0%
! CCTGTAGACCGCACCGGTAG+AGG - chr5.2:46204756-46204775 MS.gene042651:CDS 65.0%
! GTAGACCGCACCGGTAGAGG+CGG - chr5.2:46204759-46204778 MS.gene042651:CDS 65.0%
! TAGACCGCACCGGTAGAGGC+GGG - chr5.2:46204760-46204779 MS.gene042651:CDS 65.0%
AGGGCCGAGGTGTGGGAGCA+AGG + chr5.2:46205277-46205296 None:intergenic 70.0%
CGCGGCACAGCAGCTGGGAT+AGG + chr5.2:46204046-46204065 None:intergenic 70.0%
GTATGGCGCGGCACAGCAGC+TGG + chr5.2:46204052-46204071 None:intergenic 70.0%
! AGACCGCACCGGTAGAGGCG+GGG - chr5.2:46204761-46204780 MS.gene042651:CDS 70.0%
GACCCCCGCCTCTACCGGTG+CGG + chr5.2:46204767-46204786 None:intergenic 75.0%
! GACCGCACCGGTAGAGGCGG+GGG - chr5.2:46204762-46204781 MS.gene042651:CDS 75.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 46203287 46205324 46203287 ID=MS.gene042651
chr5.2 mRNA 46203287 46205324 46203287 ID=MS.gene042651.t1;Parent=MS.gene042651
chr5.2 exon 46204382 46205324 46204382 ID=MS.gene042651.t1.exon1;Parent=MS.gene042651.t1
chr5.2 CDS 46204382 46205324 46204382 ID=cds.MS.gene042651.t1;Parent=MS.gene042651.t1
chr5.2 exon 46204151 46204333 46204151 ID=MS.gene042651.t1.exon2;Parent=MS.gene042651.t1
chr5.2 CDS 46204151 46204333 46204151 ID=cds.MS.gene042651.t1;Parent=MS.gene042651.t1
chr5.2 exon 46203287 46203303 46203287 ID=MS.gene042651.t1.exon3;Parent=MS.gene042651.t1
chr5.2 CDS 46203287 46203303 46203287 ID=cds.MS.gene042651.t1;Parent=MS.gene042651.t1
Gene Sequence

>MS.gene042651

ATGGATCCTAATAGCCTTAACATCGATGGCCTTACCCTGTCTGAGGAGGGTTTAACCCTAAACCTTGCTTCTGAACCTGACACCGCTGCTGTCCTTGAACACTGTCTGATTGGTCGCGTCCTTGCTGATCGCGAGATTCAATTTGCCTACTTCAGCGAGCGTATGTCTCGCCCATGGAAGCCAGGCAAGAGGGTCACAATCACCAAGTCTGTGGCTGACCGTTATCTCTTTCAGTTCCACCACAAAGTTGATGCAGCCCGTGTTCTTGATGAAGGTCTGTGGCTTTATGATAATTTTCATATCGTCATGGATCAGATTTCCCTTGGTGTCGTTCCCACTTTTGTCCCTTTGAACAACATAGACTTTTGGGTTCAAGTTCATGGCTTACCCTGGGGATTTATCCAACCAAAAGTAGGTCAAGGCATAGGAAGTTTCTTAGGTACCTTTAAGTCGTATGATGGACGTAATTCAATCCATAGCTCCTATATGTGCATACGCGTAGCAATTGATGTGATTGTTCCTTTGAAACAAGAGTGGCGTGTCCGCTCTAGCAATGGTACTTTTGTAACTGTTAACTTTAAATATGAGAAGCTGGGTGTTTTTTGTCACAGGTGTGGATTGCTCGGACATACGGACAAGGTGTGTCCGGAGCTGTTTGAGTTGGAAGCCGACGACGGTGTCCGCAATTGGGGTGCTTATCTCAAGCCTGCTTTGCAACGTGTTGGTACTGCTGCAACAAATAGGTGGATACAGGATCCTATCCCAGCTGCTGTGCCGCGCCATACAAATGTTGTTGCTGCTTCAGTTAGCCGAAATGAAACTGTTGAAGGTGCTCCGGTTCCAAGTTTCAATGATAGGATGGTAGCTGTCCAGAATCAAATCATTGCAATCAAACATGATGTTCTTGCTGCTCAACTGGCTGTCAAAGCAAAACAAGCTGCTGGTGCGACTTCTCCTGGCCTGGTACATCTATTACCGTCCTTTTCAACAGGTAATTCAGGGTCCAACCTGCTTTCTACTCCGCCTCTGGTGCTTGGACTGCCTGCTGGTCCGTTTCCTTTTACTACTGCTGCAGACTCGCAAGAGGAAACCGGTTCTGACTTGAAGAAAAGAAAGAGGATGCTGACCCTTCAGAATGGTGACAATTCATCTTTTGCTGCTGATGGGGATGAGGGTAACAATGTAATAGGAACAAATGTTAATCATGGGGGGGATGATGATATGAATGTAATGTCTAACCCACTGTTTGATGAAACTGATGTAATGGCAGGACCTGATGATCAGGCCTGCCTAGGTAAATGAGTTTAGTGGCTTGGAATTGTCGGGGTGTAGGTAGCCCGAGTACAATTCCTGACCTTAAGTACCTAATCCGGCACTTCAATCCGGATATCTTATTCCTAAGTGAGACTCTAGCTCACAGAAATAAAATTGAAGAACTTCGTTTTTTGCTTGGTTACGATTGTTGTTTTCCTGTAGACCGCACCGGTAGAGGCGGGGGTCTTGCATTATTTTGGCGCAATTCTTTCACTTGTCAGCTTATTGATTTTTCAAGTAATCATATTACTGTTGAAATTTTTGATAGTGTTCTTGGCTCTTGGAGACTTACTGGTTACTATGGTTATCCTAATGGTGGTCGTAGAAATGCCGCTTGGAACTTTTTACGACAACTTTCAAATCAATTTACAGGCCCTTGGTGCATTTTCGGCGATTTTAATGACATTCTTGATGCGAGTGAGAAAAGGGGGCGCACCACTAGACCTCCATGGCTCATCAACGATTTCCAAAAAGCTGTTCTTGACTCTGGGTTATCCAATGTTCCGATTGAAGGTTACCCTTTCACATGGTTCAAAAGTCTTGGCACCCCACGTGCCGTCGAAGAAAGGTTAGATCGCGCACTCGCTAATAGTTTGTGGTTTAATATTTTTCCAACTGCTTATGTTGAAACTTTGGTAGCTCCTGCTTCTGATCACTATCCTATTTATGTTAACCTTGCTCCCACACCTCGGCCCTATATTGCTAAGCGCCATTTTCGTTATGA
Protein sequence

>MS.gene042651.t1

MDPNSLNIDGLTLSEEGLTLNLASEPDTAAVLEHCLIGRVLADREIQFAYFSERMSRPWKPGKRVTITKSVADRYLFQFHHKVDAARVLDEGLWLYDNFHIVMDQISLGVVPTFVPLNNIDFWVQVHGLPWGFIQPKVGQGIGSFLGTFKSYDGRNSIHSSYMCIRVAIDVIVPLKQEWRVRSSNGTFVTVNFKYEKLGVFCHRCGLLGHTDKVCPELFELEADDGVRNWGAYLKPALQRVGTAATNRWIQDPIPAAVPRHTNVVAASVSRNETVEGAPVPSFNDRMVAVQNQIIAIKHDVLAAQLAVKAKQAAGNSGSNLLSTPPLVLGLPAGPFPFTTAADSQEETGSDLKKRKRMLTLQNGDNSSFAADGDEAPFSL