AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059269


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059269.t1 MTR_3g053920 53.488 129 59 1 31 159 153 280 3.37e-34 123
MS.gene059269.t1 MTR_8g463110 57.851 121 48 2 102 221 1 119 9.55e-26 104
MS.gene059269.t1 MTR_4g103530 67.188 64 21 0 64 127 119 182 3.16e-22 90.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 56 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTACATGTACTGGTCTATA+TGG 0.263632 4.4:-74959862 MS.gene059269:CDS
ATTTGTTGCTGATGGGGATT+TGG 0.269350 4.4:-74959644 MS.gene059269:CDS
GATTTGGGAAACAATGTAAT+AGG 0.361104 4.4:-74959628 MS.gene059269:CDS
GCTCCACACGTGACTACTTT+AGG 0.367894 4.4:-74959994 MS.gene059269:CDS
CTGATGATGGTGTTAGGAAT+TGG 0.377248 4.4:-74960137 MS.gene059269:CDS
TTTGTTGCTGATGGGGATTT+GGG 0.401550 4.4:-74959643 MS.gene059269:CDS
TGATGATGGTGTTAGGAATT+GGG 0.402058 4.4:-74960136 MS.gene059269:CDS
TTTCTGCCTTCTTCTTCAAC+AGG 0.408021 4.4:-74959829 MS.gene059269:CDS
CCCAACCGACATGTGGTGCT+TGG 0.425546 4.4:-74959784 MS.gene059269:CDS
AGCATCCTGTCATTAAAATT+CGG 0.429494 4.4:+74959958 None:intergenic
CTGTCTCTTTCACACATGTT+GGG 0.432759 4.4:-74959753 MS.gene059269:CDS
TGATTGAGGTTATCTGAGTC+TGG 0.450615 4.4:+74959929 None:intergenic
CTGGTTCTGAATTGAAGAAA+AGG 0.452237 4.4:-74959702 MS.gene059269:CDS
CCAAGCACCACATGTCGGTT+GGG 0.462207 4.4:+74959784 None:intergenic
ATGTCGGTTGGGCAGCAAGC+TGG 0.463983 4.4:+74959795 None:intergenic
GCTGTCTCTTTCACACATGT+TGG 0.467225 4.4:-74959754 MS.gene059269:CDS
AAGCATATCATGTTTGATTG+AGG 0.467490 4.4:+74959915 None:intergenic
GACAATTCATTTGTTGCTGA+TGG 0.472057 4.4:-74959652 MS.gene059269:CDS
TGGACTCTGATGATGGTGTT+AGG 0.480605 4.4:-74960143 MS.gene059269:CDS
GTGCCAACACGTTGAGCAGC+AGG 0.483402 4.4:+74960099 None:intergenic
TAACGGATCAGTAACATCAA+TGG 0.488291 4.4:+74960302 None:intergenic
CAATTCATTTGTTGCTGATG+GGG 0.489906 4.4:-74959650 MS.gene059269:CDS
TCATACAGACAAGGTGTGCC+AGG 0.492374 4.4:-74960178 MS.gene059269:CDS
GTGCCAGGAGCTGTATGAAT+TGG 0.505407 4.4:-74960163 MS.gene059269:CDS
GCGACATAGTTGCAGGGATA+GGG 0.506820 4.4:+74960049 None:intergenic
ACAATTCATTTGTTGCTGAT+GGG 0.506833 4.4:-74959651 MS.gene059269:CDS
GTACATGTACTGGTCTATAT+GGG 0.511561 4.4:-74959861 MS.gene059269:CDS
GCCAAGCACCACATGTCGGT+TGG 0.511911 4.4:+74959783 None:intergenic
GAAGAAAAGGAAACGGATGC+TGG 0.515278 4.4:-74959689 MS.gene059269:CDS
CTGAATTGAAGAAAAGGAAA+CGG 0.519643 4.4:-74959696 MS.gene059269:CDS
AGTCCAATTCATACAGCTCC+TGG 0.522005 4.4:+74960160 None:intergenic
ATGAATTGTCAACATTCTGA+AGG 0.522547 4.4:+74959665 None:intergenic
TATGAATTGGACTCTGATGA+TGG 0.543306 4.4:-74960150 MS.gene059269:CDS
AAGCCTGCTGCTCAACGTGT+TGG 0.554604 4.4:-74960102 MS.gene059269:CDS
ATTAAAATTCGGCACAGTGT+CGG 0.557984 4.4:+74959969 None:intergenic
TGCGACATAGTTGCAGGGAT+AGG 0.562822 4.4:+74960048 None:intergenic
CATCAAAACAACACTACTGA+TGG 0.565999 4.4:-74960027 MS.gene059269:CDS
TAGGACCTGATGTTAGTCAT+GGG 0.567876 4.4:-74959609 MS.gene059269:CDS
TGTCACAGGTGTGGACTGAT+CGG 0.574284 4.4:-74960201 MS.gene059269:intron
CACTTATATGCGCATCAAAG+TGG 0.574766 4.4:-74960325 None:intergenic
ATAGGACCTGATGTTAGTCA+TGG 0.592916 4.4:-74959610 MS.gene059269:CDS
CAAAACAACACTACTGATGG+TGG 0.600050 4.4:-74960024 MS.gene059269:CDS
GATGATGGTGTTAGGAATTG+GGG 0.601084 4.4:-74960135 MS.gene059269:CDS
GTGTGAAAGAGACAGCTAGT+AGG 0.603076 4.4:+74959761 None:intergenic
TCACATGATGACACAGACAC+TGG 0.610799 4.4:-74959721 MS.gene059269:CDS
TGAATTGTCAACATTCTGAA+GGG 0.627752 4.4:+74959666 None:intergenic
TTTGATGCGACATAGTTGCA+GGG 0.627931 4.4:+74960043 None:intergenic
CTTGCTGCCCAACCGACATG+TGG 0.637374 4.4:-74959791 MS.gene059269:CDS
AGGACCTGATGTTAGTCATG+GGG 0.649618 4.4:-74959608 MS.gene059269:CDS
GTGCCTAAAGTAGTCACGTG+TGG 0.651126 4.4:+74959991 None:intergenic
GGACCTGATGTTAGTCATGG+GGG 0.653439 4.4:-74959607 MS.gene059269:CDS
GAGTTGCCTGTTGAAGAAGA+AGG 0.654333 4.4:+74959823 None:intergenic
GCACTGTTGCAACAAATCGA+TGG 0.661776 4.4:-74960080 MS.gene059269:CDS
GACCTGATGTTAGTCATGGG+GGG 0.672812 4.4:-74959606 MS.gene059269:CDS
GTAGGCCAAGCACCACATGT+CGG 0.685277 4.4:+74959779 None:intergenic
ACTGATCGGTCATACAGACA+AGG 0.728300 4.4:-74960187 MS.gene059269:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGCATATCATGTTTGATTG+AGG + chr4.4:74960006-74960025 None:intergenic 30.0%
AGCATCCTGTCATTAAAATT+CGG + chr4.4:74959963-74959982 None:intergenic 30.0%
ATGAATTGTCAACATTCTGA+AGG + chr4.4:74960256-74960275 None:intergenic 30.0%
CTGAATTGAAGAAAAGGAAA+CGG - chr4.4:74960222-74960241 MS.gene059269:intron 30.0%
GATTTGGGAAACAATGTAAT+AGG - chr4.4:74960290-74960309 MS.gene059269:intron 30.0%
TGAATTGTCAACATTCTGAA+GGG + chr4.4:74960255-74960274 None:intergenic 30.0%
TGTTACTGATCCGTTAAAAA+AGG - chr4.4:74959623-74959642 MS.gene059269:CDS 30.0%
! ACAATTCATTTGTTGCTGAT+GGG - chr4.4:74960267-74960286 MS.gene059269:intron 30.0%
!!! ACACACCATTCCTTTTTTAA+CGG + chr4.4:74959636-74959655 None:intergenic 30.0%
!!! ACATGTACCTTGTTTTTCTT+TGG + chr4.4:74960045-74960064 None:intergenic 30.0%
AACACTGCCAAAGAAAAACA+AGG - chr4.4:74960035-74960054 MS.gene059269:CDS 35.0%
ATTAAAATTCGGCACAGTGT+CGG + chr4.4:74959952-74959971 None:intergenic 35.0%
CATCAAAACAACACTACTGA+TGG - chr4.4:74959891-74959910 MS.gene059269:CDS 35.0%
CTGATCCGTTAAAAAAGGAA+TGG - chr4.4:74959628-74959647 MS.gene059269:CDS 35.0%
GAAAAACAAGGTACATGTAC+TGG - chr4.4:74960047-74960066 MS.gene059269:CDS 35.0%
TATGAATTGGACTCTGATGA+TGG - chr4.4:74959768-74959787 MS.gene059269:CDS 35.0%
! CAATTCATTTGTTGCTGATG+GGG - chr4.4:74960268-74960287 MS.gene059269:intron 35.0%
! GACAATTCATTTGTTGCTGA+TGG - chr4.4:74960266-74960285 MS.gene059269:intron 35.0%
! GTACATGTACTGGTCTATAT+GGG - chr4.4:74960057-74960076 MS.gene059269:CDS 35.0%
! TAACGGATCAGTAACATCAA+TGG + chr4.4:74959619-74959638 None:intergenic 35.0%
!! CTGGTTCTGAATTGAAGAAA+AGG - chr4.4:74960216-74960235 MS.gene059269:intron 35.0%
!! TGATGATGGTGTTAGGAATT+GGG - chr4.4:74959782-74959801 MS.gene059269:CDS 35.0%
ATAGGACCTGATGTTAGTCA+TGG - chr4.4:74960308-74960327 MS.gene059269:CDS 40.0%
CAAAACAACACTACTGATGG+TGG - chr4.4:74959894-74959913 MS.gene059269:CDS 40.0%
CAAAAGAACCATTGCTAGCA+CGG + chr4.4:74959659-74959678 None:intergenic 40.0%
CTGTCTCTTTCACACATGTT+GGG - chr4.4:74960165-74960184 MS.gene059269:CDS 40.0%
TAGGACCTGATGTTAGTCAT+GGG - chr4.4:74960309-74960328 MS.gene059269:CDS 40.0%
TGATTGAGGTTATCTGAGTC+TGG + chr4.4:74959992-74960011 None:intergenic 40.0%
TTTCTGCCTTCTTCTTCAAC+AGG - chr4.4:74960089-74960108 MS.gene059269:CDS 40.0%
TTTGATGCGACATAGTTGCA+GGG + chr4.4:74959878-74959897 None:intergenic 40.0%
TTTGTTGCTGATGGGGATTT+GGG - chr4.4:74960275-74960294 MS.gene059269:intron 40.0%
! AATTGGGTGTTTTCTGTCAC+AGG - chr4.4:74959703-74959722 MS.gene059269:CDS 40.0%
! ATTTGTTGCTGATGGGGATT+TGG - chr4.4:74960274-74960293 MS.gene059269:intron 40.0%
! GATGATGGTGTTAGGAATTG+GGG - chr4.4:74959783-74959802 MS.gene059269:CDS 40.0%
! GGTACATGTACTGGTCTATA+TGG - chr4.4:74960056-74960075 MS.gene059269:CDS 40.0%
! GTTTTTCTTTGGCAGTGTTC+TGG + chr4.4:74960034-74960053 None:intergenic 40.0%
! TTTTGATGCGACATAGTTGC+AGG + chr4.4:74959879-74959898 None:intergenic 40.0%
!! CTGATGATGGTGTTAGGAAT+TGG - chr4.4:74959781-74959800 MS.gene059269:CDS 40.0%
!! CTGTGCCGAATTTTAATGAC+AGG - chr4.4:74959955-74959974 MS.gene059269:CDS 40.0%
ACTGATCGGTCATACAGACA+AGG - chr4.4:74959731-74959750 MS.gene059269:CDS 45.0%
AGGACCTGATGTTAGTCATG+GGG - chr4.4:74960310-74960329 MS.gene059269:CDS 45.0%
AGTCCAATTCATACAGCTCC+TGG + chr4.4:74959761-74959780 None:intergenic 45.0%
GAAGAAAAGGAAACGGATGC+TGG - chr4.4:74960229-74960248 MS.gene059269:intron 45.0%
GAGTTGCCTGTTGAAGAAGA+AGG + chr4.4:74960098-74960117 None:intergenic 45.0%
GCACTGTTGCAACAAATCGA+TGG - chr4.4:74959838-74959857 MS.gene059269:CDS 45.0%
GCTGTCTCTTTCACACATGT+TGG - chr4.4:74960164-74960183 MS.gene059269:CDS 45.0%
GTGTGAAAGAGACAGCTAGT+AGG + chr4.4:74960160-74960179 None:intergenic 45.0%
TCACATGATGACACAGACAC+TGG - chr4.4:74960197-74960216 MS.gene059269:intron 45.0%
!! TGGACTCTGATGATGGTGTT+AGG - chr4.4:74959775-74959794 MS.gene059269:CDS 45.0%
!!! AATTTTAAATATGAAAAATT+GGG - chr4.4:74959687-74959706 MS.gene059269:CDS 5.0%
!!! TAATTTTAAATATGAAAAAT+TGG - chr4.4:74959686-74959705 MS.gene059269:CDS 5.0%
ATCCCCCCATGACTAACATC+AGG + chr4.4:74960317-74960336 None:intergenic 50.0%
GACCTGATGTTAGTCATGGG+GGG - chr4.4:74960312-74960331 MS.gene059269:CDS 50.0%
GCGACATAGTTGCAGGGATA+GGG + chr4.4:74959872-74959891 None:intergenic 50.0%
GCTCCACACGTGACTACTTT+AGG - chr4.4:74959924-74959943 MS.gene059269:CDS 50.0%
GGACCTGATGTTAGTCATGG+GGG - chr4.4:74960311-74960330 MS.gene059269:CDS 50.0%
GTGCCAGGAGCTGTATGAAT+TGG - chr4.4:74959755-74959774 MS.gene059269:CDS 50.0%
GTGCCTAAAGTAGTCACGTG+TGG + chr4.4:74959930-74959949 None:intergenic 50.0%
TCATACAGACAAGGTGTGCC+AGG - chr4.4:74959740-74959759 MS.gene059269:CDS 50.0%
TGCGACATAGTTGCAGGGAT+AGG + chr4.4:74959873-74959892 None:intergenic 50.0%
! GGTGTTTTCTGTCACAGGTG+TGG - chr4.4:74959708-74959727 MS.gene059269:CDS 50.0%
! TGTCACAGGTGTGGACTGAT+CGG - chr4.4:74959717-74959736 MS.gene059269:CDS 50.0%
AAGCCTGCTGCTCAACGTGT+TGG - chr4.4:74959816-74959835 MS.gene059269:CDS 55.0%
GTAGGCCAAGCACCACATGT+CGG + chr4.4:74960142-74960161 None:intergenic 55.0%
TGGTGTGTCCGTGCTAGCAA+TGG - chr4.4:74959648-74959667 MS.gene059269:CDS 55.0%
! CCAAGCACCACATGTCGGTT+GGG + chr4.4:74960137-74960156 None:intergenic 55.0%
CCCAACCGACATGTGGTGCT+TGG - chr4.4:74960134-74960153 MS.gene059269:CDS 60.0%
CTTGCTGCCCAACCGACATG+TGG - chr4.4:74960127-74960146 MS.gene059269:CDS 60.0%
GCCAAGCACCACATGTCGGT+TGG + chr4.4:74960138-74960157 None:intergenic 60.0%
GTGCCAACACGTTGAGCAGC+AGG + chr4.4:74959822-74959841 None:intergenic 60.0%
! ATGTCGGTTGGGCAGCAAGC+TGG + chr4.4:74960126-74960145 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 74959600 74960340 74959600 ID=MS.gene059269
chr4.4 mRNA 74959600 74960340 74959600 ID=MS.gene059269.t1;Parent=MS.gene059269
chr4.4 exon 74960291 74960340 74960291 ID=MS.gene059269.t1.exon1;Parent=MS.gene059269.t1
chr4.4 CDS 74960291 74960340 74960291 ID=cds.MS.gene059269.t1;Parent=MS.gene059269.t1
chr4.4 exon 74959600 74960215 74959600 ID=MS.gene059269.t1.exon2;Parent=MS.gene059269.t1
chr4.4 CDS 74959600 74960215 74959600 ID=cds.MS.gene059269.t1;Parent=MS.gene059269.t1
Gene Sequence

>MS.gene059269

ATGCGCATCAAAGTGGCCATTGATGTTACTGATCCGTTAAAAAAGGAATGGTGTGTCCGTGCTAGCAATGGTTCTTTTGTGACTGTTAATTTTAAATATGAAAAATTGGGTGTTTTCTGTCACAGGTGTGGACTGATCGGTCATACAGACAAGGTGTGCCAGGAGCTGTATGAATTGGACTCTGATGATGGTGTTAGGAATTGGGGTGTTTATTTGAAGCCTGCTGCTCAACGTGTTGGCACTGTTGCAACAAATCGATGGCTGCAAGACCCTATCCCTGCAACTATGTCGCATCAAAACAACACTACTGATGGTGGTTTTGCTGCTCCACACGTGACTACTTTAGGCACCGACACTGTGCCGAATTTTAATGACAGGATGCTTGTTGTCCAGACTCAGATAACCTCAATCAAACATGATATGCTTGCTGTCCAGAACACTGCCAAAGAAAAACAAGGTACATGTACTGGTCTATATGGGCAAATGCACTTTCTGCCTTCTTCTTCAACAGGCAACTCGATCTCCAGCTTGCTGCCCAACCGACATGTGGTGCTTGGCCTACTAGCTGTCTCTTTCACACATGTTGGGTGTGATGAATCACATGATGACACAGACACTGGTTCTGAATTGAAGAAAAGGAAACGGATGCTGGCCCTTCAGAATGTTGACAATTCATTTGTTGCTGATGGGGATTTGGGAAACAATGTAATAGGACCTGATGTTAGTCATGGGGGGATGTAG

Protein sequence

>MS.gene059269.t1

MRIKVAIDVTDPLKKEWCGLIGHTDKVCQELYELDSDDGVRNWGVYLKPAAQRVGTVATNRWLQDPIPATMSHQNNTTDGGFAAPHVTTLGTDTVPNFNDRMLVVQTQITSIKHDMLAVQNTAKEKQGTCTGLYGQMHFLPSSSTGNSISSLLPNRHVVLGLLAVSFTHVGCDESHDDTDTGSELKKRKRMLALQNVDNSFVADGDLGNNVIGPDVSHGGM