AlfalfaGEDB | MS.gene29406

M. sativa cultivar XinJiangDaYe / MS.gene29406

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29406.t1	AES70414.1	52.6	346	95	4	47	389	1	280	4.80E-89	338.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29406.t1	G7J017	52.6	346	95	4	47	389	1	280	3.5e-89	338.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene29406	MS.gene61651	PPI
MS.gene049512	MS.gene29406	PPI
MS.gene038623	MS.gene29406	PPI
MS.gene05357	MS.gene29406	PPI
MS.gene87529	MS.gene29406	PPI
MS.gene29406	MS.gene09023	PPI
MS.gene04134	MS.gene29406	PPI
MS.gene29406	MS.gene054508	PPI
MS.gene29406	MS.gene025987	PPI
MS.gene062067	MS.gene29406	PPI
MS.gene29406	MS.gene95960	PPI
MS.gene29406	MS.gene053373	PPI
MS.gene29406	MS.gene043140	PPI
MS.gene29406	MS.gene041264	PPI
MS.gene57044	MS.gene29406	PPI
MS.gene39956	MS.gene29406	PPI
MS.gene29406	MS.gene65474	PPI
MS.gene71637	MS.gene29406	PPI
MS.gene29406	MS.gene025401	PPI
MS.gene09589	MS.gene29406	PPI
MS.gene29406	MS.gene015093	PPI
MS.gene29406	MS.gene50392	PPI
MS.gene29406	MS.gene22730	PPI
MS.gene29406	MS.gene24278	PPI
MS.gene29406	MS.gene000379	PPI
MS.gene29406	MS.gene032299	PPI
MS.gene29406	MS.gene08713	PPI
MS.gene29406	MS.gene09517	PPI
MS.gene29406	MS.gene87845	PPI
MS.gene060386	MS.gene29406	PPI
MS.gene71588	MS.gene29406	PPI
MS.gene04749	MS.gene29406	PPI
MS.gene29406	MS.gene62249	PPI
MS.gene29406	MS.gene58419	PPI
MS.gene059736	MS.gene29406	PPI
MS.gene42903	MS.gene29406	PPI
MS.gene81712	MS.gene29406	PPI
MS.gene29406	MS.gene05576	PPI
MS.gene026680	MS.gene29406	PPI
MS.gene29406	MS.gene044729	PPI
MS.gene021555	MS.gene29406	PPI
MS.gene048747	MS.gene29406	PPI
MS.gene29406	MS.gene60390	PPI
MS.gene29406	MS.gene48477	PPI
MS.gene29406	MS.gene039692	PPI
MS.gene22200	MS.gene29406	PPI
MS.gene29406	MS.gene027169	PPI
MS.gene29406	MS.gene55682	PPI
MS.gene29406	MS.gene019177	PPI
MS.gene29406	MS.gene28718	PPI
MS.gene94654	MS.gene29406	PPI
MS.gene29406	MS.gene055560	PPI
MS.gene031967	MS.gene29406	PPI
MS.gene29406	MS.gene024297	PPI
MS.gene02896	MS.gene29406	PPI
MS.gene29406	MS.gene057934	PPI
MS.gene29406	MS.gene37537	PPI
MS.gene063214	MS.gene29406	PPI
MS.gene29406	MS.gene024841	PPI
MS.gene97410	MS.gene29406	PPI
MS.gene83772	MS.gene29406	PPI
MS.gene32415	MS.gene29406	PPI
MS.gene29406	MS.gene069786	PPI
MS.gene035626	MS.gene29406	PPI
MS.gene29406	MS.gene42010	PPI
MS.gene29406	MS.gene025158	PPI
MS.gene29406	MS.gene40828	PPI
MS.gene29406	MS.gene27329	PPI
MS.gene29406	MS.gene41393	PPI
MS.gene63762	MS.gene29406	PPI
MS.gene65623	MS.gene29406	PPI
MS.gene98471	MS.gene29406	PPI
MS.gene71675	MS.gene29406	PPI
MS.gene30208	MS.gene29406	PPI
MS.gene69495	MS.gene29406	PPI
MS.gene29406	MS.gene016632	PPI
MS.gene29406	MS.gene40469	PPI
MS.gene032311	MS.gene29406	PPI
MS.gene030090	MS.gene29406	PPI
MS.gene96174	MS.gene29406	PPI
MS.gene018196	MS.gene29406	PPI
MS.gene86849	MS.gene29406	PPI
MS.gene57019	MS.gene29406	PPI
MS.gene29406	MS.gene03149	PPI
MS.gene29406	MS.gene29321	PPI
MS.gene03936	MS.gene29406	PPI
MS.gene021968	MS.gene29406	PPI
MS.gene29406	MS.gene037049	PPI
MS.gene60059	MS.gene29406	PPI
MS.gene29406	MS.gene026680	PPI
MS.gene43393	MS.gene29406	PPI
MS.gene29406	MS.gene34618	PPI
MS.gene34197	MS.gene29406	PPI
MS.gene29406	MS.gene59460	PPI
MS.gene29406	MS.gene019165	PPI
MS.gene29406	MS.gene81194	PPI
MS.gene008275	MS.gene29406	PPI
MS.gene29406	MS.gene060773	PPI
MS.gene29406	MS.gene66650	PPI
MS.gene038757	MS.gene29406	PPI
MS.gene005120	MS.gene29406	PPI
MS.gene29406	MS.gene038394	PPI
MS.gene29406	MS.gene019001	PPI
MS.gene048072	MS.gene29406	PPI
MS.gene29406	MS.gene53904	PPI
MS.gene29406	MS.gene24070	PPI
MS.gene29406	MS.gene73261	PPI
MS.gene29406	MS.gene85538	PPI
MS.gene05436	MS.gene29406	PPI
MS.gene29406	MS.gene26844	PPI
MS.gene29406	MS.gene64178	PPI
MS.gene29406	MS.gene055546	PPI
MS.gene70932	MS.gene29406	PPI
MS.gene047954	MS.gene29406	PPI
MS.gene35175	MS.gene29406	PPI
MS.gene29406	MS.gene55209	PPI
MS.gene29406	MS.gene074257	PPI
MS.gene29406	MS.gene073573	PPI
MS.gene29406	MS.gene44144	PPI
MS.gene021965	MS.gene29406	PPI
MS.gene055683	MS.gene29406	PPI
MS.gene042614	MS.gene29406	PPI
MS.gene29406	MS.gene060718	PPI
MS.gene66651	MS.gene29406	PPI
MS.gene96109	MS.gene29406	PPI
MS.gene29406	MS.gene068629	PPI
MS.gene29406	MS.gene004417	PPI
MS.gene35202	MS.gene29406	PPI
MS.gene29406	MS.gene009781	PPI
MS.gene051449	MS.gene29406	PPI
MS.gene009121	MS.gene29406	PPI
MS.gene48477	MS.gene29406	PPI
MS.gene29406	MS.gene21066	PPI
MS.gene29406	MS.gene009213	PPI
MS.gene29406	MS.gene013890	PPI
MS.gene36069	MS.gene29406	PPI
MS.gene29406	MS.gene98127	PPI
MS.gene29406	MS.gene003264	PPI
MS.gene29406	MS.gene054652	PPI
MS.gene30136	MS.gene29406	PPI
MS.gene61452	MS.gene29406	PPI
MS.gene008927	MS.gene29406	PPI
MS.gene016102	MS.gene29406	PPI
MS.gene46941	MS.gene29406	PPI
MS.gene045614	MS.gene29406	PPI
MS.gene96541	MS.gene29406	PPI
MS.gene000634	MS.gene29406	PPI
MS.gene29406	MS.gene80362	PPI
MS.gene29406	MS.gene49057	PPI
MS.gene29406	MS.gene89815	PPI
MS.gene46948	MS.gene29406	PPI
MS.gene025873	MS.gene29406	PPI
MS.gene29406	MS.gene018474	PPI
MS.gene29406	MS.gene048088	PPI
MS.gene29406	MS.gene86868	PPI
MS.gene012019	MS.gene29406	PPI
MS.gene013676	MS.gene29406	PPI
MS.gene05358	MS.gene29406	PPI
MS.gene29406	MS.gene058889	PPI
MS.gene29406	MS.gene96163	PPI
MS.gene95561	MS.gene29406	PPI
MS.gene29406	MS.gene034643	PPI
MS.gene065627	MS.gene29406	PPI
MS.gene018745	MS.gene29406	PPI
MS.gene000629	MS.gene29406	PPI
MS.gene65546	MS.gene29406	PPI
MS.gene29406	MS.gene012889	PPI
MS.gene29406	MS.gene34463	PPI
MS.gene05319	MS.gene29406	PPI
MS.gene038098	MS.gene29406	PPI
MS.gene56991	MS.gene29406	PPI
MS.gene29406	MS.gene61499	PPI
MS.gene29406	MS.gene51804	PPI
MS.gene28545	MS.gene29406	PPI
MS.gene28718	MS.gene29406	PPI
MS.gene064966	MS.gene29406	PPI
MS.gene29406	MS.gene025675	PPI
MS.gene29406	MS.gene04970	PPI
MS.gene98616	MS.gene29406	PPI
MS.gene43924	MS.gene29406	PPI
MS.gene046089	MS.gene29406	PPI
MS.gene29406	MS.gene71637	PPI
MS.gene036423	MS.gene29406	PPI
MS.gene29406	MS.gene43924	PPI
MS.gene29406	MS.gene022929	PPI
MS.gene29406	MS.gene26433	PPI
MS.gene80362	MS.gene29406	PPI
MS.gene29406	MS.gene007022	PPI
MS.gene72520	MS.gene29406	PPI
MS.gene013193	MS.gene29406	PPI
MS.gene29406	MS.gene69584	PPI
MS.gene29406	MS.gene52349	PPI
MS.gene29406	MS.gene021647	PPI
MS.gene63743	MS.gene29406	PPI
MS.gene29406	MS.gene048342	PPI
MS.gene29406	MS.gene069624	PPI
MS.gene29406	MS.gene073582	PPI
MS.gene008861	MS.gene29406	PPI
MS.gene024405	MS.gene29406	PPI
MS.gene29406	MS.gene016719	PPI
MS.gene29406	MS.gene028714	PPI
MS.gene012748	MS.gene29406	PPI
MS.gene29406	MS.gene88393	PPI
MS.gene29406	MS.gene048706	PPI
MS.gene83979	MS.gene29406	PPI
MS.gene29406	MS.gene69586	PPI
MS.gene29406	MS.gene45083	PPI
MS.gene29406	MS.gene049561	PPI
MS.gene29406	MS.gene008658	PPI
MS.gene85295	MS.gene29406	PPI
MS.gene29406	MS.gene63150	PPI
MS.gene47792	MS.gene29406	PPI
MS.gene29406	MS.gene03936	PPI
MS.gene038676	MS.gene29406	PPI
MS.gene29406	MS.gene072852	PPI
MS.gene042651	MS.gene29406	PPI
MS.gene29406	MS.gene08708	PPI
MS.gene93396	MS.gene29406	PPI
MS.gene05576	MS.gene29406	PPI
MS.gene29406	MS.gene06164	PPI
MS.gene29406	MS.gene86275	PPI
MS.gene05231	MS.gene29406	PPI
MS.gene29406	MS.gene017586	PPI
MS.gene29406	MS.gene037634	PPI
MS.gene29406	MS.gene057828	PPI
MS.gene010656	MS.gene29406	PPI
MS.gene29406	MS.gene08606	PPI
MS.gene034643	MS.gene29406	PPI
MS.gene041264	MS.gene29406	PPI
MS.gene41979	MS.gene29406	PPI
MS.gene29406	MS.gene000640	PPI
MS.gene29406	MS.gene038757	PPI
MS.gene29406	MS.gene000506	PPI
MS.gene29406	MS.gene98756	PPI
MS.gene64130	MS.gene29406	PPI
MS.gene050679	MS.gene29406	PPI
MS.gene29406	MS.gene028716	PPI
MS.gene29406	MS.gene74639	PPI
MS.gene29406	MS.gene071133	PPI
MS.gene29406	MS.gene60266	PPI
MS.gene20246	MS.gene29406	PPI
MS.gene055223	MS.gene29406	PPI
MS.gene29406	MS.gene98467	PPI
MS.gene29406	MS.gene013644	PPI
MS.gene29406	MS.gene024522	PPI
MS.gene29406	MS.gene54051	PPI
MS.gene071784	MS.gene29406	PPI
MS.gene053747	MS.gene29406	PPI
MS.gene29406	MS.gene22111	PPI
MS.gene29406	MS.gene049610	PPI
MS.gene29406	MS.gene010290	PPI
MS.gene061579	MS.gene29406	PPI
MS.gene29406	MS.gene37955	PPI
MS.gene76420	MS.gene29406	PPI
MS.gene071320	MS.gene29406	PPI
MS.gene65471	MS.gene29406	PPI
MS.gene061850	MS.gene29406	PPI
MS.gene29406	MS.gene92517	PPI
MS.gene29406	MS.gene36069	PPI
MS.gene29406	MS.gene045875	PPI
MS.gene29406	MS.gene010890	PPI
MS.gene29406	MS.gene84790	PPI
MS.gene26433	MS.gene29406	PPI
MS.gene29406	MS.gene061579	PPI
MS.gene042559	MS.gene29406	PPI
MS.gene29406	MS.gene002590	PPI
MS.gene26038	MS.gene29406	PPI
MS.gene29406	MS.gene060788	PPI
MS.gene042027	MS.gene29406	PPI
MS.gene85308	MS.gene29406	PPI
MS.gene29406	MS.gene41643	PPI
MS.gene69490	MS.gene29406	PPI
MS.gene29406	MS.gene77990	PPI
MS.gene29406	MS.gene062511	PPI
MS.gene015248	MS.gene29406	PPI
MS.gene29406	MS.gene027994	PPI
MS.gene29406	MS.gene059269	PPI
MS.gene34812	MS.gene29406	PPI
MS.gene44083	MS.gene29406	PPI
MS.gene04970	MS.gene29406	PPI
MS.gene08106	MS.gene29406	PPI
MS.gene58419	MS.gene29406	PPI
MS.gene29406	MS.gene76420	PPI
MS.gene29406	MS.gene46353	PPI
MS.gene067340	MS.gene29406	PPI
MS.gene29406	MS.gene51799	PPI
MS.gene29406	MS.gene98471	PPI
MS.gene23521	MS.gene29406	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29406.t1	MTR_3g053920	52.601	346	95	4	47	389	1	280	2.16e-111	330
MS.gene29406.t1	MTR_2g061500	75.194	129	32	0	47	175	1	129	3.16e-68	214
MS.gene29406.t1	MTR_7g011710	81.818	88	16	0	101	188	1	88	1.50e-46	159
MS.gene29406.t1	MTR_4g103530	66.337	101	33	1	101	201	1	100	3.90e-42	148
MS.gene29406.t1	MTR_4g103530	46.875	64	34	0	297	360	119	182	7.82e-11	61.6
MS.gene29406.t1	MTR_8g463110	70.339	118	32	1	335	449	1	118	1.27e-35	140
MS.gene29406.t1	MTR_6g033215	35.260	173	110	1	75	245	122	294	2.58e-34	130
MS.gene29406.t1	MTR_0010s0370	36.207	174	101	6	57	223	7	177	5.21e-28	110
MS.gene29406.t1	MTR_0010s0350	36.207	174	101	6	57	223	7	177	5.21e-28	110
MS.gene29406.t1	MTR_7g106180	26.000	150	103	3	78	221	19	166	2.59e-14	72.8
MS.gene29406.t1	MTR_1g036720	30.769	104	70	1	57	158	7	110	1.77e-12	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29406.t1	AT2G17920	27.907	215	145	5	54	262	9	219	1.61e-18	86.7
MS.gene29406.t1	AT2G41590	27.439	164	115	2	101	262	58	219	1.29e-16	81.6
MS.gene29406.t1	AT5G18636	27.442	215	146	4	54	262	9	219	3.37e-16	80.5
MS.gene29406.t1	AT5G25200	27.442	215	146	4	54	262	9	219	3.40e-16	80.5
MS.gene29406.t1	AT3G57587	28.378	148	102	2	117	262	74	219	3.47e-16	80.5
MS.gene29406.t1	AT2G02103	27.907	215	145	4	54	262	9	219	5.71e-16	80.1
MS.gene29406.t1	AT5G36228	27.083	192	136	2	82	271	39	228	5.93e-16	79.7
MS.gene29406.t1	AT3G31430	26.374	182	128	3	82	261	134	311	2.86e-14	74.3
MS.gene29406.t1	AT2G13450	25.543	184	129	4	82	261	39	218	7.43e-14	73.6
MS.gene29406.t1	AT3G47920	25.000	148	107	2	117	262	3	148	1.01e-12	69.3

Find 144 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTTCAATTTCATCACAAAA+TGG	0.275930	3.3:-31657953	MS.gene29406:CDS
CAGACTCGGTGGCTAGGTTC+AGG	0.306454	3.3:+31658124	None:intergenic
TCGGTAAGAGATAGAGAAAC+AGG	0.309778	3.3:+31658207	None:intergenic
ACCTGAACCATTTGGACATC+TGG	0.323453	3.3:-31657834	MS.gene29406:CDS
CAGGTTCACGGTCTTCCTTT+TGG	0.325576	3.3:-31657808	MS.gene29406:CDS
CTTAGCCGACCGTGAGATTC+AGG	0.334402	3.3:-31658061	MS.gene29406:CDS
GACTCCTCAGAGTTGGTATT+AGG	0.336786	3.3:+31657127	None:intergenic
TACGGGCAGCATTAGGCTTC+AGG	0.352023	3.3:+31657482	None:intergenic
TCAGATTGTGTTTCACGGTT+AGG	0.362563	3.3:+31658276	None:intergenic
GCAGCATTAGGCTTCAGGTC+AGG	0.367623	3.3:+31657487	None:intergenic
ATCCTTGATCACTGTTTGAT+TGG	0.375534	3.3:-31658090	MS.gene29406:CDS
TTGGTGCTTGGATCACCTGC+TGG	0.383837	3.3:-31657157	MS.gene29406:CDS
ACGATCGAAACACGCTTGCC+TGG	0.389839	3.3:+31658000	None:intergenic
CAGATTGTGTTTCACGGTTA+GGG	0.390851	3.3:+31658277	None:intergenic
AGCCATACGCTCGCTGAAAT+AGG	0.391985	3.3:+31658035	None:intergenic
CCCAGATGTCCAAATGGTTC+AGG	0.397477	3.3:+31657833	None:intergenic
CCTTCTCACCTCAATATTGC+TGG	0.397709	3.3:-31658174	MS.gene29406:CDS
CACGACATAGGTAGCTTTCT+AGG	0.402316	3.3:-31657763	MS.gene29406:CDS
GAAACAGGGAGGAAGACAAT+AGG	0.405034	3.3:+31658222	None:intergenic
GTTGGCCGCCCCTTGGTGCT+TGG	0.411391	3.3:-31657169	MS.gene29406:CDS
AATGGCAGGCCTTGATGATC+AGG	0.412415	3.3:-31656921	MS.gene29406:CDS
GACATCTGGGTCCAGGTTCA+CGG	0.413519	3.3:-31657820	MS.gene29406:CDS
CTCTATCGACCTGAACCATT+TGG	0.415630	3.3:-31657842	MS.gene29406:CDS
AAACCAATACGGGCAGCATT+AGG	0.417335	3.3:+31657475	None:intergenic
TCGGCTTCAAATTTGATTGT+TGG	0.420945	3.3:-31657187	MS.gene29406:CDS
AAGCCTAATGCTGCCCGTAT+TGG	0.422074	3.3:-31657478	MS.gene29406:CDS
CCTATGTCGTGACCAACATT+TGG	0.425346	3.3:+31657775	None:intergenic
TCAAACGCATATAGGTACTA+TGG	0.426389	3.3:+31657707	None:intergenic
ATGGCTCGTGCTTGGAAGCC+AGG	0.426942	3.3:-31658018	MS.gene29406:CDS
ATGATGATGGTGTTCGCAAT+TGG	0.426984	3.3:-31657513	MS.gene29406:CDS
TGTTTCAGGTGCGGTTTGCT+TGG	0.429250	3.3:-31657577	MS.gene29406:CDS
AAACAGGGAGGAAGACAATA+GGG	0.434502	3.3:+31658223	None:intergenic
ACGGACTTTCAAACGCATAT+AGG	0.445879	3.3:+31657699	None:intergenic
TACCACTAGGAGGAGAGTTT+CGG	0.448317	3.3:+31657368	None:intergenic
ACGGACCGTCATCGAGAATA+CGG	0.448638	3.3:+31657923	None:intergenic
TATGCTGGTGTCTCTTCTGC+TGG	0.452739	3.3:-31657391	MS.gene29406:CDS
CCACTCCTTTGAAGAAAGAA+TGG	0.453354	3.3:-31657666	MS.gene29406:CDS
TGATGATGGTGTTCGCAATT+GGG	0.461183	3.3:-31657512	MS.gene29406:CDS
CAAAAGGAAGACCGTGAACC+TGG	0.461333	3.3:+31657809	None:intergenic
CGCGGCACAACAGAAGGGAT+TGG	0.461403	3.3:+31657424	None:intergenic
GATGAAATTGAAACAGAAAT+CGG	0.463191	3.3:+31657962	None:intergenic
GATAGAGTTACGGACATCAT+AGG	0.469134	3.3:+31657729	None:intergenic
AGACTCGGTGGCTAGGTTCA+GGG	0.469866	3.3:+31658125	None:intergenic
TCTATCTCTTACCGATTCCA+TGG	0.471695	3.3:-31658199	MS.gene29406:CDS
GCGGCACAACAGAAGGGATT+GGG	0.475065	3.3:+31657425	None:intergenic
TATTAGCAGCAAAACCAATA+CGG	0.478257	3.3:+31657464	None:intergenic
CAGACTCAGATTGTGTTTCA+CGG	0.481156	3.3:+31658271	None:intergenic
CAGCATTAGGCTTCAGGTCA+GGG	0.485216	3.3:+31657488	None:intergenic
TGGTCTCACCCTTGCTGAAG+AGG	0.487281	3.3:-31658154	MS.gene29406:CDS
GGTCTCACCCTTGCTGAAGA+GGG	0.487511	3.3:-31658153	MS.gene29406:CDS
GAGGAGTCTCAAGATGCTAC+TGG	0.487924	3.3:-31657112	MS.gene29406:CDS
TGCCTATTTCAGCGAGCGTA+TGG	0.489265	3.3:-31658037	MS.gene29406:CDS
TTTGATTGTTGGCCGCCCCT+TGG	0.490362	3.3:-31657176	MS.gene29406:CDS
TACTCCAACTCGAACAACTC+CGG	0.491131	3.3:+31657535	None:intergenic
ACTTTCCATTCTTTCTTCAA+AGG	0.491536	3.3:+31657661	None:intergenic
CCATTTGGACATCTGGGTCC+AGG	0.493863	3.3:-31657827	MS.gene29406:CDS
TTGCCCGGAGTTGTTCGAGT+TGG	0.498205	3.3:-31657539	MS.gene29406:CDS
TGGTTCAGGTCGATAGAGGC+AGG	0.498541	3.3:+31657847	None:intergenic
CCATTCTTTCTTCAAAGGAG+TGG	0.499164	3.3:+31657666	None:intergenic
TGTTGGCGCGGCACAACAGA+AGG	0.501754	3.3:+31657418	None:intergenic
GCGAGCGTATGGCTCGTGCT+TGG	0.503834	3.3:-31658026	MS.gene29406:CDS
CAAATGGTTCAGGTCGATAG+AGG	0.510517	3.3:+31657843	None:intergenic
TTTGGTTGAATGCAGCCAAA+AGG	0.511595	3.3:+31657793	None:intergenic
CAGCAAGTAGATCATGTTTG+AGG	0.515254	3.3:+31657287	None:intergenic
TAGGAACAAATGTTAGTCAT+GGG	0.515296	3.3:-31656997	MS.gene29406:CDS
CGATAATCTCCACATCGTAA+TGG	0.517010	3.3:-31657893	MS.gene29406:CDS
AGGTACTATGGATAGAGTTA+CGG	0.517799	3.3:+31657719	None:intergenic
CCTGGACCCAGATGTCCAAA+TGG	0.518153	3.3:+31657827	None:intergenic
GGCTGCATTCAACCAAATGT+TGG	0.520543	3.3:-31657787	MS.gene29406:CDS
TTCTTCTTCTACAGGTAACT+CGG	0.521316	3.3:-31657206	MS.gene29406:CDS
ACACACTGACAAGGTTTGCC+CGG	0.528306	3.3:-31657554	MS.gene29406:CDS
CCAAATGTTGGTCACGACAT+AGG	0.530107	3.3:-31657775	MS.gene29406:CDS
GTTTGATGACACTGATGTAA+TGG	0.531460	3.3:-31656939	MS.gene29406:CDS
CTGAACTGAAGAAGAGAAAG+AGG	0.535515	3.3:-31657087	MS.gene29406:CDS
GGCCAATCAAACAGTGATCA+AGG	0.536687	3.3:+31658088	None:intergenic
GTATTAGGAAACGGTCCAGC+AGG	0.537381	3.3:+31657142	None:intergenic
GGACAACACCGGGTTGCATA+CGG	0.540333	3.3:+31657869	None:intergenic
ATAGGAACAAATGTTAGTCA+TGG	0.541273	3.3:-31656998	MS.gene29406:CDS
GAAGAAGAGAAAGAGGATGC+TGG	0.542242	3.3:-31657080	MS.gene29406:CDS
CTAGCCTCATTTACCTAGGC+AGG	0.542385	3.3:+31656898	None:intergenic
ATGTTTCAGACTCGGTGGCT+AGG	0.544692	3.3:+31658118	None:intergenic
CTTGATGATCAGGCCTGCCT+AGG	0.545915	3.3:-31656911	MS.gene29406:CDS
GCAGGTGATCCAAGCACCAA+GGG	0.551957	3.3:+31657160	None:intergenic
AGCAAGTAGATCATGTTTGA+GGG	0.552412	3.3:+31657288	None:intergenic
TCAGAGTTGGTATTAGGAAA+CGG	0.553022	3.3:+31657133	None:intergenic
GTGAGAAGGATCCATGGAAT+CGG	0.553139	3.3:+31658188	None:intergenic
TTCGAGTTGGAGTATGATGA+TGG	0.553333	3.3:-31657526	MS.gene29406:CDS
ATTAGCAGCAAAACCAATAC+GGG	0.555064	3.3:+31657465	None:intergenic
CAGGCCTGCCTAGGTAAATG+AGG	0.555791	3.3:-31656902	None:intergenic
GTCAAAGCAAAACGTGACTC+AGG	0.555861	3.3:-31657253	MS.gene29406:CDS
AAGGAGTGGTGACATTAATA+CGG	0.558516	3.3:+31657680	None:intergenic
TGGAAAGTCCGCGTCAGTAA+TGG	0.560762	3.3:-31657646	MS.gene29406:CDS
CCGCGCCAACATGTTTATGC+TGG	0.561526	3.3:-31657406	MS.gene29406:CDS
GGGTTAAACCCTCTTCAGCA+AGG	0.563127	3.3:+31658145	None:intergenic
ATCTTGAGACTCCTCAGAGT+TGG	0.563156	3.3:+31657120	None:intergenic
ACTCCAACTCGAACAACTCC+GGG	0.565052	3.3:+31657536	None:intergenic
CCAGCAATATTGAGGTGAGA+AGG	0.566066	3.3:+31658174	None:intergenic
CCTGAACCATTTGGACATCT+GGG	0.566438	3.3:-31657833	MS.gene29406:CDS
GAGTTACCTGTAGAAGAAGA+AGG	0.567956	3.3:+31657208	None:intergenic
GGGTGAGACCAGCAATATTG+AGG	0.568160	3.3:+31658166	None:intergenic
AGGATGCTGGCTCTTCAGAA+TGG	0.570182	3.3:-31657067	MS.gene29406:CDS
CTAGGCAGGCCTGATCATCA+AGG	0.575743	3.3:+31656912	None:intergenic
GATTGTCAACAGCAAGCATA+CGG	0.578956	3.3:+31657320	None:intergenic
CGGACCGTCATCGAGAATAC+GGG	0.590350	3.3:+31657924	None:intergenic
ATTGAGGTGAGAAGGATCCA+TGG	0.594735	3.3:+31658182	None:intergenic
GATGATGGTGTTCGCAATTG+GGG	0.596979	3.3:-31657511	MS.gene29406:CDS
AGCAGGTGATCCAAGCACCA+AGG	0.598268	3.3:+31657159	None:intergenic
CAAACAGTGATCAAGGATGG+TGG	0.603272	3.3:+31658095	None:intergenic
ATAGAGGCAGGGACAACACC+GGG	0.603779	3.3:+31657859	None:intergenic
GATGGTGGATGTTTCAGACT+CGG	0.616645	3.3:+31658110	None:intergenic
AACAAATGTTAGTCATGGGG+GGG	0.619233	3.3:-31656993	MS.gene29406:CDS
CGGTAAGAGATAGAGAAACA+GGG	0.623032	3.3:+31658208	None:intergenic
AATCAAACAGTGATCAAGGA+TGG	0.623412	3.3:+31658092	None:intergenic
GGCAGCCTGAATCTCACGGT+CGG	0.624448	3.3:+31658056	None:intergenic
AATAGGCAGCCTGAATCTCA+CGG	0.627231	3.3:+31658052	None:intergenic
AAGCAGACAATAGTTGACAT+TGG	0.628524	3.3:+31658321	None:intergenic
TTTGCTTGGACACACTGACA+AGG	0.630531	3.3:-31657563	MS.gene29406:CDS
GTGATCCAAGCACCAAGGGG+CGG	0.631081	3.3:+31657164	None:intergenic
GATGACACTGATGTAATGGC+AGG	0.632333	3.3:-31656935	MS.gene29406:CDS
GGTTAAACCCTCTTCAGCAA+GGG	0.635778	3.3:+31658146	None:intergenic
AGGAACAAATGTTAGTCATG+GGG	0.636254	3.3:-31656996	MS.gene29406:CDS
GTATTCTCGATGACGGTCCG+TGG	0.636653	3.3:-31657921	MS.gene29406:CDS
GGCCGAAACTCTCCTCCTAG+TGG	0.636967	3.3:-31657370	MS.gene29406:CDS
GTAATGGACCGTATGCAACC+CGG	0.639014	3.3:-31657877	MS.gene29406:CDS
GATAGAGGCAGGGACAACAC+CGG	0.643119	3.3:+31657858	None:intergenic
GCTGCCCGTATTCTCGATGA+CGG	0.643477	3.3:-31657928	MS.gene29406:CDS
AGACACCAGCATAAACATGT+TGG	0.646971	3.3:+31657401	None:intergenic
GGAACAAATGTTAGTCATGG+GGG	0.656011	3.3:-31656995	MS.gene29406:CDS
CCAGCATAAACATGTTGGCG+CGG	0.656583	3.3:+31657406	None:intergenic
TCTCACGGTCGGCTAAGACA+CGG	0.658392	3.3:+31658067	None:intergenic
GGTTCAGGTCGATAGAGGCA+GGG	0.658904	3.3:+31657848	None:intergenic
GAACAAATGTTAGTCATGGG+GGG	0.658931	3.3:-31656994	MS.gene29406:CDS
GTTGGCGCGGCACAACAGAA+GGG	0.660636	3.3:+31657419	None:intergenic
GCCACTAGCCTCATTTACCT+AGG	0.663986	3.3:+31656894	None:intergenic
CATAAGCACCATTACTGACG+CGG	0.666254	3.3:+31657638	None:intergenic
GGTGGATGTTTCAGACTCGG+TGG	0.677598	3.3:+31658113	None:intergenic
TGGAGATTATCGTACAGCCA+CGG	0.684427	3.3:+31657904	None:intergenic
GCAGCATTGACAGTACCACT+AGG	0.687827	3.3:+31657355	None:intergenic
GCATTGACAGTACCACTAGG+AGG	0.712834	3.3:+31657358	None:intergenic
ACATCAGTGTCATCAAACAG+AGG	0.714525	3.3:+31656944	None:intergenic
CAGGTGATCCAAGCACCAAG+GGG	0.715495	3.3:+31657161	None:intergenic
TAAGAGATAGAGAAACAGGG+AGG	0.733444	3.3:+31658211	None:intergenic
GTTTCCTAATACCAACTCTG+AGG	0.734723	3.3:-31657131	MS.gene29406:CDS
GCATACGGTCCATTACGATG+TGG	0.736469	3.3:+31657884	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ACTTTTAAATATGAGAAACT+TGG	-	chr3.3:31657615-31657634	MS.gene29406:CDS	20.0%
!!!	TTTTGCTTTGACAATATTTT+GGG	+	chr3.3:31657961-31657980	None:intergenic	20.0%
!	GATGAAATTGAAACAGAAAT+CGG	+	chr3.3:31657263-31657282	None:intergenic	25.0%
!	GTTTCAATTTCATCACAAAA+TGG	-	chr3.3:31657269-31657288	MS.gene29406:CDS	25.0%
!!	GTTTTGCTTTGACAATATTT+TGG	+	chr3.3:31657962-31657981	None:intergenic	25.0%
	ACTTTCCATTCTTTCTTCAA+AGG	+	chr3.3:31657564-31657583	None:intergenic	30.0%
	ATAGGAACAAATGTTAGTCA+TGG	-	chr3.3:31658224-31658243	MS.gene29406:CDS	30.0%
	TAGGAACAAATGTTAGTCAT+GGG	-	chr3.3:31658225-31658244	MS.gene29406:CDS	30.0%
	TATTAGCAGCAAAACCAATA+CGG	+	chr3.3:31657761-31657780	None:intergenic	30.0%
!	GTTTTGCTGCTAATAACAAA+TGG	-	chr3.3:31657766-31657785	MS.gene29406:CDS	30.0%
!!!	AACTTGGAGTTTTTTGTTTC+AGG	-	chr3.3:31657631-31657650	MS.gene29406:CDS	30.0%
	AAGGAGTGGTGACATTAATA+CGG	+	chr3.3:31657545-31657564	None:intergenic	35.0%
	AATCAAACAGTGATCAAGGA+TGG	+	chr3.3:31657133-31657152	None:intergenic	35.0%
	AGCAAGTAGATCATGTTTGA+GGG	+	chr3.3:31657937-31657956	None:intergenic	35.0%
	AGGAACAAATGTTAGTCATG+GGG	-	chr3.3:31658226-31658245	MS.gene29406:CDS	35.0%
	AGGTACTATGGATAGAGTTA+CGG	+	chr3.3:31657506-31657525	None:intergenic	35.0%
	ATCCTTGATCACTGTTTGAT+TGG	-	chr3.3:31657132-31657151	MS.gene29406:CDS	35.0%
	ATTAGCAGCAAAACCAATAC+GGG	+	chr3.3:31657760-31657779	None:intergenic	35.0%
	TCAAACGCATATAGGTACTA+TGG	+	chr3.3:31657518-31657537	None:intergenic	35.0%
	TCGGCTTCAAATTTGATTGT+TGG	-	chr3.3:31658035-31658054	MS.gene29406:CDS	35.0%
	TTCTTCTTCTACAGGTAACT+CGG	-	chr3.3:31658016-31658035	MS.gene29406:CDS	35.0%
!	ATTTCATCTTTTGCTGCTGA+TGG	-	chr3.3:31658182-31658201	MS.gene29406:CDS	35.0%
!	CTTTTACCTTCTTCTTCTAC+AGG	-	chr3.3:31658008-31658027	MS.gene29406:CDS	35.0%
!	GGTGAGGGAAATTTTGTAAT+AGG	-	chr3.3:31658206-31658225	MS.gene29406:CDS	35.0%
!	GTTTGATGACACTGATGTAA+TGG	-	chr3.3:31658283-31658302	MS.gene29406:CDS	35.0%
!	TTTCATCTTTTGCTGCTGAT+GGG	-	chr3.3:31658183-31658202	MS.gene29406:CDS	35.0%
!!	TCAGAGTTGGTATTAGGAAA+CGG	+	chr3.3:31658092-31658111	None:intergenic	35.0%
	AAACAGGGAGGAAGACAATA+GGG	+	chr3.3:31657002-31657021	None:intergenic	40.0%
	AACAAATGTTAGTCATGGGG+GGG	-	chr3.3:31658229-31658248	MS.gene29406:CDS	40.0%
	ACATCAGTGTCATCAAACAG+AGG	+	chr3.3:31658281-31658300	None:intergenic	40.0%
	ACGGACTTTCAAACGCATAT+AGG	+	chr3.3:31657526-31657545	None:intergenic	40.0%
	AGACACCAGCATAAACATGT+TGG	+	chr3.3:31657824-31657843	None:intergenic	40.0%
	CAGACTCAGATTGTGTTTCA+CGG	+	chr3.3:31656954-31656973	None:intergenic	40.0%
	CAGATTGTGTTTCACGGTTA+GGG	+	chr3.3:31656948-31656967	None:intergenic	40.0%
	CAGCAAGTAGATCATGTTTG+AGG	+	chr3.3:31657938-31657957	None:intergenic	40.0%
	CCATTCTTTCTTCAAAGGAG+TGG	+	chr3.3:31657559-31657578	None:intergenic	40.0%
	CGATAATCTCCACATCGTAA+TGG	-	chr3.3:31657329-31657348	MS.gene29406:CDS	40.0%
	CGGTAAGAGATAGAGAAACA+GGG	+	chr3.3:31657017-31657036	None:intergenic	40.0%
	CTGAACTGAAGAAGAGAAAG+AGG	-	chr3.3:31658135-31658154	MS.gene29406:CDS	40.0%
	GAACAAATGTTAGTCATGGG+GGG	-	chr3.3:31658228-31658247	MS.gene29406:CDS	40.0%
	GAGTTACCTGTAGAAGAAGA+AGG	+	chr3.3:31658017-31658036	None:intergenic	40.0%
	GATAGAGTTACGGACATCAT+AGG	+	chr3.3:31657496-31657515	None:intergenic	40.0%
	GATTGTCAACAGCAAGCATA+CGG	+	chr3.3:31657905-31657924	None:intergenic	40.0%
	GGAACAAATGTTAGTCATGG+GGG	-	chr3.3:31658227-31658246	MS.gene29406:CDS	40.0%
	GGAAGACAATAGGGAAAAAG+CGG	+	chr3.3:31656993-31657012	None:intergenic	40.0%
	GTTTCCTAATACCAACTCTG+AGG	-	chr3.3:31658091-31658110	MS.gene29406:CDS	40.0%
	TAAGAGATAGAGAAACAGGG+AGG	+	chr3.3:31657014-31657033	None:intergenic	40.0%
	TCAGATTGTGTTTCACGGTT+AGG	+	chr3.3:31656949-31656968	None:intergenic	40.0%
	TCGGTAAGAGATAGAGAAAC+AGG	+	chr3.3:31657018-31657037	None:intergenic	40.0%
	TTTGGTTGAATGCAGCCAAA+AGG	+	chr3.3:31657432-31657451	None:intergenic	40.0%
!	CCACTCCTTTGAAGAAAGAA+TGG	-	chr3.3:31657556-31657575	MS.gene29406:CDS	40.0%
!	TTCATCTTTTGCTGCTGATG+GGG	-	chr3.3:31658184-31658203	MS.gene29406:CDS	40.0%
!!	ATGATGATGGTGTTCGCAAT+TGG	-	chr3.3:31657709-31657728	MS.gene29406:CDS	40.0%
!!	TCTATCTCTTACCGATTCCA+TGG	-	chr3.3:31657023-31657042	MS.gene29406:CDS	40.0%
!!	TGATGATGGTGTTCGCAATT+GGG	-	chr3.3:31657710-31657729	MS.gene29406:CDS	40.0%
!!	TTCGAGTTGGAGTATGATGA+TGG	-	chr3.3:31657696-31657715	MS.gene29406:CDS	40.0%
!!!	GGAGTTTTTTGTTTCAGGTG+CGG	-	chr3.3:31657636-31657655	MS.gene29406:CDS	40.0%
	AAACCAATACGGGCAGCATT+AGG	+	chr3.3:31657750-31657769	None:intergenic	45.0%
	AATAGGCAGCCTGAATCTCA+CGG	+	chr3.3:31657173-31657192	None:intergenic	45.0%
	ACCTGAACCATTTGGACATC+TGG	-	chr3.3:31657388-31657407	MS.gene29406:CDS	45.0%
	ATCTTGAGACTCCTCAGAGT+TGG	+	chr3.3:31658105-31658124	None:intergenic	45.0%
	ATTGAGGTGAGAAGGATCCA+TGG	+	chr3.3:31657043-31657062	None:intergenic	45.0%
	CAAACAGTGATCAAGGATGG+TGG	+	chr3.3:31657130-31657149	None:intergenic	45.0%
	CAAATGGTTCAGGTCGATAG+AGG	+	chr3.3:31657382-31657401	None:intergenic	45.0%
	CACGACATAGGTAGCTTTCT+AGG	-	chr3.3:31657459-31657478	MS.gene29406:CDS	45.0%
	CATAAGCACCATTACTGACG+CGG	+	chr3.3:31657587-31657606	None:intergenic	45.0%
	CCAAATGTTGGTCACGACAT+AGG	-	chr3.3:31657447-31657466	MS.gene29406:CDS	45.0%
	CCAGCAATATTGAGGTGAGA+AGG	+	chr3.3:31657051-31657070	None:intergenic	45.0%
	CCTATGTCGTGACCAACATT+TGG	+	chr3.3:31657450-31657469	None:intergenic	45.0%
	CCTGAACCATTTGGACATCT+GGG	-	chr3.3:31657389-31657408	MS.gene29406:CDS	45.0%
	CCTTCTCACCTCAATATTGC+TGG	-	chr3.3:31657048-31657067	MS.gene29406:CDS	45.0%
	CTCTATCGACCTGAACCATT+TGG	-	chr3.3:31657380-31657399	MS.gene29406:CDS	45.0%
	GAAACAGGGAGGAAGACAAT+AGG	+	chr3.3:31657003-31657022	None:intergenic	45.0%
	GAAGAAGAGAAAGAGGATGC+TGG	-	chr3.3:31658142-31658161	MS.gene29406:CDS	45.0%
	GATGACACTGATGTAATGGC+AGG	-	chr3.3:31658287-31658306	MS.gene29406:CDS	45.0%
	GGCCAATCAAACAGTGATCA+AGG	+	chr3.3:31657137-31657156	None:intergenic	45.0%
	GGCTGCATTCAACCAAATGT+TGG	-	chr3.3:31657435-31657454	MS.gene29406:CDS	45.0%
	GGTTAAACCCTCTTCAGCAA+GGG	+	chr3.3:31657079-31657098	None:intergenic	45.0%
	GTCAAAGCAAAACGTGACTC+AGG	-	chr3.3:31657969-31657988	MS.gene29406:CDS	45.0%
	GTGAGAAGGATCCATGGAAT+CGG	+	chr3.3:31657037-31657056	None:intergenic	45.0%
	TACCACTAGGAGGAGAGTTT+CGG	+	chr3.3:31657857-31657876	None:intergenic	45.0%
	TACTCCAACTCGAACAACTC+CGG	+	chr3.3:31657690-31657709	None:intergenic	45.0%
	TGGAGATTATCGTACAGCCA+CGG	+	chr3.3:31657321-31657340	None:intergenic	45.0%
	TTTGCTTGGACACACTGACA+AGG	-	chr3.3:31657659-31657678	MS.gene29406:CDS	45.0%
!	GATGATGGTGTTCGCAATTG+GGG	-	chr3.3:31657711-31657730	MS.gene29406:CDS	45.0%
!	GTTTCACGGTTAGGGTTTTC+TGG	+	chr3.3:31656940-31656959	None:intergenic	45.0%
!	TCATCTTTTGCTGCTGATGG+GGG	-	chr3.3:31658185-31658204	MS.gene29406:CDS	45.0%
!!	GACTCCTCAGAGTTGGTATT+AGG	+	chr3.3:31658098-31658117	None:intergenic	45.0%
!!	GATGGTGGATGTTTCAGACT+CGG	+	chr3.3:31657115-31657134	None:intergenic	45.0%
!!!	ACGGTTAGGGTTTTCTGGTT+TGG	+	chr3.3:31656935-31656954	None:intergenic	45.0%
	AAGCCTAATGCTGCCCGTAT+TGG	-	chr3.3:31657744-31657763	MS.gene29406:CDS	50.0%
	ACACACTGACAAGGTTTGCC+CGG	-	chr3.3:31657668-31657687	MS.gene29406:CDS	50.0%
	ACGGACCGTCATCGAGAATA+CGG	+	chr3.3:31657302-31657321	None:intergenic	50.0%
	ACTCCAACTCGAACAACTCC+GGG	+	chr3.3:31657689-31657708	None:intergenic	50.0%
	AGCCATACGCTCGCTGAAAT+AGG	+	chr3.3:31657190-31657209	None:intergenic	50.0%
	CAAAAGGAAGACCGTGAACC+TGG	+	chr3.3:31657416-31657435	None:intergenic	50.0%
	CAGCATTAGGCTTCAGGTCA+GGG	+	chr3.3:31657737-31657756	None:intergenic	50.0%
	CCAGCATAAACATGTTGGCG+CGG	+	chr3.3:31657819-31657838	None:intergenic	50.0%
	CCCAGATGTCCAAATGGTTC+AGG	+	chr3.3:31657392-31657411	None:intergenic	50.0%
	GAGGAGTCTCAAGATGCTAC+TGG	-	chr3.3:31658110-31658129	MS.gene29406:CDS	50.0%
	GCATACGGTCCATTACGATG+TGG	+	chr3.3:31657341-31657360	None:intergenic	50.0%
	GGGTGAGACCAGCAATATTG+AGG	+	chr3.3:31657059-31657078	None:intergenic	50.0%
	GGGTTAAACCCTCTTCAGCA+AGG	+	chr3.3:31657080-31657099	None:intergenic	50.0%
	GTATTAGGAAACGGTCCAGC+AGG	+	chr3.3:31658083-31658102	None:intergenic	50.0%
	TATGCTGGTGTCTCTTCTGC+TGG	-	chr3.3:31657831-31657850	MS.gene29406:CDS	50.0%
	TGCCTATTTCAGCGAGCGTA+TGG	-	chr3.3:31657185-31657204	MS.gene29406:CDS	50.0%
	TGGAAAGTCCGCGTCAGTAA+TGG	-	chr3.3:31657576-31657595	MS.gene29406:CDS	50.0%
!	AATGGCAGGCCTTGATGATC+AGG	-	chr3.3:31658301-31658320	MS.gene29406:CDS	50.0%
!	CAGGTTCACGGTCTTCCTTT+TGG	-	chr3.3:31657414-31657433	MS.gene29406:CDS	50.0%
!	GCATTGACAGTACCACTAGG+AGG	+	chr3.3:31657867-31657886	None:intergenic	50.0%
!	TGTTTCAGGTGCGGTTTGCT+TGG	-	chr3.3:31657645-31657664	MS.gene29406:CDS	50.0%
!!	AGGATGCTGGCTCTTCAGAA+TGG	-	chr3.3:31658155-31658174	MS.gene29406:CDS	50.0%
!!	ATGTTTCAGACTCGGTGGCT+AGG	+	chr3.3:31657107-31657126	None:intergenic	50.0%
!!	GCAGCATTGACAGTACCACT+AGG	+	chr3.3:31657870-31657889	None:intergenic	50.0%
!!	GTAATGGACCGTATGCAACC+CGG	-	chr3.3:31657345-31657364	MS.gene29406:CDS	50.0%
	ACGATCGAAACACGCTTGCC+TGG	+	chr3.3:31657225-31657244	None:intergenic	55.0%
	AGCAGGTGATCCAAGCACCA+AGG	+	chr3.3:31658066-31658085	None:intergenic	55.0%
	ATAGAGGCAGGGACAACACC+GGG	+	chr3.3:31657366-31657385	None:intergenic	55.0%
	CAGGTGATCCAAGCACCAAG+GGG	+	chr3.3:31658064-31658083	None:intergenic	55.0%
	CCATTTGGACATCTGGGTCC+AGG	-	chr3.3:31657395-31657414	MS.gene29406:CDS	55.0%
	CCGCGCCAACATGTTTATGC+TGG	-	chr3.3:31657816-31657835	MS.gene29406:CDS	55.0%
	CCTGGACCCAGATGTCCAAA+TGG	+	chr3.3:31657398-31657417	None:intergenic	55.0%
	CGGACCGTCATCGAGAATAC+GGG	+	chr3.3:31657301-31657320	None:intergenic	55.0%
	CTAGGCAGGCCTGATCATCA+AGG	+	chr3.3:31658313-31658332	None:intergenic	55.0%
	CTTAGCCGACCGTGAGATTC+AGG	-	chr3.3:31657161-31657180	MS.gene29406:CDS	55.0%
	GACATCTGGGTCCAGGTTCA+CGG	-	chr3.3:31657402-31657421	MS.gene29406:CDS	55.0%
	GATAGAGGCAGGGACAACAC+CGG	+	chr3.3:31657367-31657386	None:intergenic	55.0%
	GCAGCATTAGGCTTCAGGTC+AGG	+	chr3.3:31657738-31657757	None:intergenic	55.0%
	GCAGGTGATCCAAGCACCAA+GGG	+	chr3.3:31658065-31658084	None:intergenic	55.0%
	GCGGCACAACAGAAGGGATT+GGG	+	chr3.3:31657800-31657819	None:intergenic	55.0%
	GCTGCCCGTATTCTCGATGA+CGG	-	chr3.3:31657294-31657313	MS.gene29406:CDS	55.0%
	GGACAACACCGGGTTGCATA+CGG	+	chr3.3:31657356-31657375	None:intergenic	55.0%
	GGTCTCACCCTTGCTGAAGA+GGG	-	chr3.3:31657069-31657088	MS.gene29406:CDS	55.0%
	GGTGGATGTTTCAGACTCGG+TGG	+	chr3.3:31657112-31657131	None:intergenic	55.0%
	GGTTCAGGTCGATAGAGGCA+GGG	+	chr3.3:31657377-31657396	None:intergenic	55.0%
	TACGGGCAGCATTAGGCTTC+AGG	+	chr3.3:31657743-31657762	None:intergenic	55.0%
	TCTCACGGTCGGCTAAGACA+CGG	+	chr3.3:31657158-31657177	None:intergenic	55.0%
	TGGTCTCACCCTTGCTGAAG+AGG	-	chr3.3:31657068-31657087	MS.gene29406:CDS	55.0%
	TGGTTCAGGTCGATAGAGGC+AGG	+	chr3.3:31657378-31657397	None:intergenic	55.0%
	TTGCCCGGAGTTGTTCGAGT+TGG	-	chr3.3:31657683-31657702	MS.gene29406:CDS	55.0%
	TTTGATTGTTGGCCGCCCCT+TGG	-	chr3.3:31658046-31658065	MS.gene29406:CDS	55.0%
	TTTGCTGCTGATGGGGGTGA+GGG	-	chr3.3:31658191-31658210	MS.gene29406:CDS	55.0%
!	CTTGATGATCAGGCCTGCCT+AGG	-	chr3.3:31658311-31658330	MS.gene29406:CDS	55.0%
!	GTATTCTCGATGACGGTCCG+TGG	-	chr3.3:31657301-31657320	MS.gene29406:CDS	55.0%
!	TTGGTGCTTGGATCACCTGC+TGG	-	chr3.3:31658065-31658084	MS.gene29406:CDS	55.0%
!	TTTTGCTGCTGATGGGGGTG+AGG	-	chr3.3:31658190-31658209	MS.gene29406:CDS	55.0%
!!	AGACTCGGTGGCTAGGTTCA+GGG	+	chr3.3:31657100-31657119	None:intergenic	55.0%
	ATGGCTCGTGCTTGGAAGCC+AGG	-	chr3.3:31657204-31657223	MS.gene29406:CDS	60.0%
	CGCGGCACAACAGAAGGGAT+TGG	+	chr3.3:31657801-31657820	None:intergenic	60.0%
	GGCAGCCTGAATCTCACGGT+CGG	+	chr3.3:31657169-31657188	None:intergenic	60.0%
	GGCCGAAACTCTCCTCCTAG+TGG	-	chr3.3:31657852-31657871	MS.gene29406:CDS	60.0%
	GTGATCCAAGCACCAAGGGG+CGG	+	chr3.3:31658061-31658080	None:intergenic	60.0%
	GTTGGCGCGGCACAACAGAA+GGG	+	chr3.3:31657806-31657825	None:intergenic	60.0%
	TGTTGGCGCGGCACAACAGA+AGG	+	chr3.3:31657807-31657826	None:intergenic	60.0%
!!	CAGACTCGGTGGCTAGGTTC+AGG	+	chr3.3:31657101-31657120	None:intergenic	60.0%
	GCGAGCGTATGGCTCGTGCT+TGG	-	chr3.3:31657196-31657215	MS.gene29406:CDS	65.0%
!!	GTTGGCCGCCCCTTGGTGCT+TGG	-	chr3.3:31658053-31658072	MS.gene29406:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	31656904	31658340	31656904	ID=MS.gene29406
chr3.3	mRNA	31656904	31658340	31656904	ID=MS.gene29406.t1;Parent=MS.gene29406
chr3.3	exon	31656904	31658340	31656904	ID=MS.gene29406.t1.exon1;Parent=MS.gene29406.t1
chr3.3	CDS	31656904	31658340	31656904	ID=cds.MS.gene29406.t1;Parent=MS.gene29406.t1

Gene Sequence

>MS.gene29406

ATGTCAACTATTGTCTGCTTCATGCTTTCCAAACCAGAAAACCCTAACCGTGAAACACAATCTGAGTCTGACATAGTTTCTGCTCTCCGCTTTTTCCCTATTGTCTTCCTCCCTGTTTCTCTATCTCTTACCGATTCCATGGATCCTTCTCACCTCAATATTGCTGGTCTCACCCTTGCTGAAGAGGGTTTAACCCTGAACCTAGCCACCGAGTCTGAAACATCCACCATCCTTGATCACTGTTTGATTGGCCGTGTCTTAGCCGACCGTGAGATTCAGGCTGCCTATTTCAGCGAGCGTATGGCTCGTGCTTGGAAGCCAGGCAAGCGTGTTTCGATCGTCAAAACTGATTGTGACCGATTTCTGTTTCAATTTCATCACAAAATGGATGCTGCCCGTATTCTCGATGACGGTCCGTGGCTGTACGATAATCTCCACATCGTAATGGACCGTATGCAACCCGGTGTTGTCCCTGCCTCTATCGACCTGAACCATTTGGACATCTGGGTCCAGGTTCACGGTCTTCCTTTTGGCTGCATTCAACCAAATGTTGGTCACGACATAGGTAGCTTTCTAGGTGAACTCAAATCCTATGATGTCCGTAACTCTATCCATAGTACCTATATGCGTTTGAAAGTCCGTATTAATGTCACCACTCCTTTGAAGAAAGAATGGAAAGTCCGCGTCAGTAATGGTGCTTATGTTACATTAACTTTTAAATATGAGAAACTTGGAGTTTTTTGTTTCAGGTGCGGTTTGCTTGGACACACTGACAAGGTTTGCCCGGAGTTGTTCGAGTTGGAGTATGATGATGGTGTTCGCAATTGGGGCCCTGACCTGAAGCCTAATGCTGCCCGTATTGGTTTTGCTGCTAATAACAAATGGTTGCAAGACCCAATCCCTTCTGTTGTGCCGCGCCAACATGTTTATGCTGGTGTCTCTTCTGCTGGCCGAAACTCTCCTCCTAGTGGTACTGTCAATGCTGCGACTTTTAGTGACCGTATGCTTGCTGTTGACAATCAGATTTCTGCCCTCAAACATGATCTACTTGCTGCCCAAAATATTGTCAAAGCAAAACGTGACTCAGGTGCTTGTCAGATACATCTTTTACCTTCTTCTTCTACAGGTAACTCGGCTTCAAATTTGATTGTTGGCCGCCCCTTGGTGCTTGGATCACCTGCTGGACCGTTTCCTAATACCAACTCTGAGGAGTCTCAAGATGCTACTGGTTCTGAACTGAAGAAGAGAAAGAGGATGCTGGCTCTTCAGAATGGTGAAATTTCATCTTTTGCTGCTGATGGGGGTGAGGGAAATTTTGTAATAGGAACAAATGTTAGTCATGGGGGGGATGTTGATATGAATGTAATTTCTAATCCTCTGTTTGATGACACTGATGTAATGGCAGGCCTTGATGATCAGGCCTGCCTAGGTAAATGA

Protein sequence

>MS.gene29406.t1

MSTIVCFMLSKPENPNRETQSESDIVSALRFFPIVFLPVSLSLTDSMDPSHLNIAGLTLAEEGLTLNLATESETSTILDHCLIGRVLADREIQAAYFSERMARAWKPGKRVSIVKTDCDRFLFQFHHKMDAARILDDGPWLYDNLHIVMDRMQPGVVPASIDLNHLDIWVQVHGLPFGCIQPNVGHDIGSFLGELKSYDVRNSIHSTYMRLKVRINVTTPLKKEWKVRVSNGAYVTLTFKYEKLGVFCFRCGLLGHTDKVCPELFELEYDDGVRNWGPDLKPNAARIGFAANNKWLQDPIPSVVPRQHVYAGVSSAGRNSPPSGTVNAATFSDRMLAVDNQISALKHDLLAAQNIVKAKRDSGACQIHLLPSSSTGNSASNLIVGRPLVLGSPAGPFPNTNSEESQDATGSELKKRKRMLALQNGEISSFAADGGEGNFVIGTNVSHGGDVDMNVISNPLFDDTDVMAGLDDQACLGK