AlfalfaGEDB | MS.gene000640

M. sativa cultivar XinJiangDaYe / MS.gene000640

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000640.t1	AES70414.1	49.6	276	40	3	1	227	55	280	8.00E-55	224.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000640.t1	G7J017	49.6	276	40	3	1	227	55	280	5.8e-55	224.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene026680	MS.gene000640	PPI
MS.gene048747	MS.gene000640	PPI
MS.gene22200	MS.gene000640	PPI
MS.gene055546	MS.gene000640	PPI
MS.gene26038	MS.gene000640	PPI
MS.gene08606	MS.gene000640	PPI
MS.gene49057	MS.gene000640	PPI
MS.gene073573	MS.gene000640	PPI
MS.gene031967	MS.gene000640	PPI
MS.gene053373	MS.gene000640	PPI
MS.gene34812	MS.gene000640	PPI
MS.gene02896	MS.gene000640	PPI
MS.gene063214	MS.gene000640	PPI
MS.gene55682	MS.gene000640	PPI
MS.gene97410	MS.gene000640	PPI
MS.gene32415	MS.gene000640	PPI
MS.gene009213	MS.gene000640	PPI
MS.gene060788	MS.gene000640	PPI
MS.gene035626	MS.gene000640	PPI
MS.gene067340	MS.gene000640	PPI
MS.gene000640	MS.gene022929	PPI
MS.gene65623	MS.gene000640	PPI
MS.gene27329	MS.gene000640	PPI
MS.gene71675	MS.gene000640	PPI
MS.gene019177	MS.gene000640	PPI
MS.gene69495	MS.gene000640	PPI
MS.gene049512	MS.gene000640	PPI
MS.gene038623	MS.gene000640	PPI
MS.gene55209	MS.gene000640	PPI
MS.gene05357	MS.gene000640	PPI
MS.gene032311	MS.gene000640	PPI
MS.gene000640	MS.gene048342	PPI
MS.gene64178	MS.gene000640	PPI
MS.gene004417	MS.gene000640	PPI
MS.gene030090	MS.gene000640	PPI
MS.gene96174	MS.gene000640	PPI
MS.gene000640	MS.gene21066	PPI
MS.gene86849	MS.gene000640	PPI
MS.gene50392	MS.gene000640	PPI
MS.gene072852	MS.gene000640	PPI
MS.gene41393	MS.gene000640	PPI
MS.gene39956	MS.gene000640	PPI
MS.gene021968	MS.gene000640	PPI
MS.gene000640	MS.gene008658	PPI
MS.gene09589	MS.gene000640	PPI
MS.gene44144	MS.gene000640	PPI
MS.gene43393	MS.gene000640	PPI
MS.gene057828	MS.gene000640	PPI
MS.gene34197	MS.gene000640	PPI
MS.gene66650	MS.gene000640	PPI
MS.gene005120	MS.gene000640	PPI
MS.gene86868	MS.gene000640	PPI
MS.gene048072	MS.gene000640	PPI
MS.gene060386	MS.gene000640	PPI
MS.gene71588	MS.gene000640	PPI
MS.gene04749	MS.gene000640	PPI
MS.gene05436	MS.gene000640	PPI
MS.gene059736	MS.gene000640	PPI
MS.gene70932	MS.gene000640	PPI
MS.gene35175	MS.gene000640	PPI
MS.gene62249	MS.gene000640	PPI
MS.gene81194	MS.gene000640	PPI
MS.gene025675	MS.gene000640	PPI
MS.gene000640	MS.gene74639	PPI
MS.gene000640	MS.gene071133	PPI
MS.gene069786	MS.gene000640	PPI
MS.gene021965	MS.gene000640	PPI
MS.gene055683	MS.gene000640	PPI
MS.gene042614	MS.gene000640	PPI
MS.gene41643	MS.gene000640	PPI
MS.gene013890	MS.gene000640	PPI
MS.gene26844	MS.gene000640	PPI
MS.gene021555	MS.gene000640	PPI
MS.gene060773	MS.gene000640	PPI
MS.gene021647	MS.gene000640	PPI
MS.gene000640	MS.gene37955	PPI
MS.gene96109	MS.gene000640	PPI
MS.gene69584	MS.gene000640	PPI
MS.gene019165	MS.gene000640	PPI
MS.gene048706	MS.gene000640	PPI
MS.gene015093	MS.gene000640	PPI
MS.gene007022	MS.gene000640	PPI
MS.gene000640	MS.gene84790	PPI
MS.gene009121	MS.gene000640	PPI
MS.gene94654	MS.gene000640	PPI
MS.gene024841	MS.gene000640	PPI
MS.gene51804	MS.gene000640	PPI
MS.gene87845	MS.gene000640	PPI
MS.gene36069	MS.gene000640	PPI
MS.gene95960	MS.gene000640	PPI
MS.gene61452	MS.gene000640	PPI
MS.gene83772	MS.gene000640	PPI
MS.gene008927	MS.gene000640	PPI
MS.gene016102	MS.gene000640	PPI
MS.gene46941	MS.gene000640	PPI
MS.gene045614	MS.gene000640	PPI
MS.gene96541	MS.gene000640	PPI
MS.gene049561	MS.gene000640	PPI
MS.gene000640	MS.gene027994	PPI
MS.gene000634	MS.gene000640	PPI
MS.gene009781	MS.gene000640	PPI
MS.gene63762	MS.gene000640	PPI
MS.gene000640	MS.gene007022	PPI
MS.gene30208	MS.gene000640	PPI
MS.gene028716	MS.gene000640	PPI
MS.gene21066	MS.gene000640	PPI
MS.gene025873	MS.gene000640	PPI
MS.gene012019	MS.gene000640	PPI
MS.gene06164	MS.gene000640	PPI
MS.gene013676	MS.gene000640	PPI
MS.gene05358	MS.gene000640	PPI
MS.gene95561	MS.gene000640	PPI
MS.gene074257	MS.gene000640	PPI
MS.gene018196	MS.gene000640	PPI
MS.gene000640	MS.gene61651	PPI
MS.gene065627	MS.gene000640	PPI
MS.gene57019	MS.gene000640	PPI
MS.gene010890	MS.gene000640	PPI
MS.gene018745	MS.gene000640	PPI
MS.gene65546	MS.gene000640	PPI
MS.gene09023	MS.gene000640	PPI
MS.gene059269	MS.gene000640	PPI
MS.gene000506	MS.gene000640	PPI
MS.gene000640	MS.gene88393	PPI
MS.gene74639	MS.gene000640	PPI
MS.gene032299	MS.gene000640	PPI
MS.gene03936	MS.gene000640	PPI
MS.gene038098	MS.gene000640	PPI
MS.gene56991	MS.gene000640	PPI
MS.gene60059	MS.gene000640	PPI
MS.gene062511	MS.gene000640	PPI
MS.gene22111	MS.gene000640	PPI
MS.gene28545	MS.gene000640	PPI
MS.gene28718	MS.gene000640	PPI
MS.gene055560	MS.gene000640	PPI
MS.gene008275	MS.gene000640	PPI
MS.gene29321	MS.gene000640	PPI
MS.gene34618	MS.gene000640	PPI
MS.gene000640	MS.gene015093	PPI
MS.gene59460	MS.gene000640	PPI
MS.gene038757	MS.gene000640	PPI
MS.gene064966	MS.gene000640	PPI
MS.gene069624	MS.gene000640	PPI
MS.gene000640	MS.gene000379	PPI
MS.gene016632	MS.gene000640	PPI
MS.gene002590	MS.gene000640	PPI
MS.gene025987	MS.gene000640	PPI
MS.gene000640	MS.gene08606	PPI
MS.gene000640	MS.gene09517	PPI
MS.gene40828	MS.gene000640	PPI
MS.gene43924	MS.gene000640	PPI
MS.gene046089	MS.gene000640	PPI
MS.gene047954	MS.gene000640	PPI
MS.gene77990	MS.gene000640	PPI
MS.gene036423	MS.gene000640	PPI
MS.gene054652	MS.gene000640	PPI
MS.gene000640	MS.gene62249	PPI
MS.gene013644	MS.gene000640	PPI
MS.gene84790	MS.gene000640	PPI
MS.gene72520	MS.gene000640	PPI
MS.gene61651	MS.gene000640	PPI
MS.gene66651	MS.gene000640	PPI
MS.gene24278	MS.gene000640	PPI
MS.gene35202	MS.gene000640	PPI
MS.gene025158	MS.gene000640	PPI
MS.gene051449	MS.gene000640	PPI
MS.gene61499	MS.gene000640	PPI
MS.gene019001	MS.gene000640	PPI
MS.gene63743	MS.gene000640	PPI
MS.gene000640	MS.gene60390	PPI
MS.gene63150	MS.gene000640	PPI
MS.gene48477	MS.gene000640	PPI
MS.gene045875	MS.gene000640	PPI
MS.gene54051	MS.gene000640	PPI
MS.gene038394	MS.gene000640	PPI
MS.gene30136	MS.gene000640	PPI
MS.gene008861	MS.gene000640	PPI
MS.gene73261	MS.gene000640	PPI
MS.gene012748	MS.gene000640	PPI
MS.gene024297	MS.gene000640	PPI
MS.gene83979	MS.gene000640	PPI
MS.gene45083	MS.gene000640	PPI
MS.gene92517	MS.gene000640	PPI
MS.gene85295	MS.gene000640	PPI
MS.gene057934	MS.gene000640	PPI
MS.gene60390	MS.gene000640	PPI
MS.gene47792	MS.gene000640	PPI
MS.gene46948	MS.gene000640	PPI
MS.gene98471	MS.gene000640	PPI
MS.gene049610	MS.gene000640	PPI
MS.gene060718	MS.gene000640	PPI
MS.gene000640	MS.gene025158	PPI
MS.gene025401	MS.gene000640	PPI
MS.gene93396	MS.gene000640	PPI
MS.gene05576	MS.gene000640	PPI
MS.gene52349	MS.gene000640	PPI
MS.gene05231	MS.gene000640	PPI
MS.gene46353	MS.gene000640	PPI
MS.gene010656	MS.gene000640	PPI
MS.gene000629	MS.gene000640	PPI
MS.gene42010	MS.gene000640	PPI
MS.gene40469	MS.gene000640	PPI
MS.gene041264	MS.gene000640	PPI
MS.gene41979	MS.gene000640	PPI
MS.gene000640	MS.gene025987	PPI
MS.gene024522	MS.gene000640	PPI
MS.gene048342	MS.gene000640	PPI
MS.gene64130	MS.gene000640	PPI
MS.gene000640	MS.gene043140	PPI
MS.gene05319	MS.gene000640	PPI
MS.gene88393	MS.gene000640	PPI
MS.gene050679	MS.gene000640	PPI
MS.gene000640	MS.gene03149	PPI
MS.gene85538	MS.gene000640	PPI
MS.gene20246	MS.gene000640	PPI
MS.gene055223	MS.gene000640	PPI
MS.gene016719	MS.gene000640	PPI
MS.gene010290	MS.gene000640	PPI
MS.gene053747	MS.gene000640	PPI
MS.gene65471	MS.gene000640	PPI
MS.gene061850	MS.gene000640	PPI
MS.gene000640	MS.gene24278	PPI
MS.gene26433	MS.gene000640	PPI
MS.gene34463	MS.gene000640	PPI
MS.gene048088	MS.gene000640	PPI
MS.gene008658	MS.gene000640	PPI
MS.gene000640	MS.gene59460	PPI
MS.gene018474	MS.gene000640	PPI
MS.gene22730	MS.gene000640	PPI
MS.gene003264	MS.gene000640	PPI
MS.gene073582	MS.gene000640	PPI
MS.gene042559	MS.gene000640	PPI
MS.gene058889	MS.gene000640	PPI
MS.gene98616	MS.gene000640	PPI
MS.gene071133	MS.gene000640	PPI
MS.gene53904	MS.gene000640	PPI
MS.gene08708	MS.gene000640	PPI
MS.gene09517	MS.gene000640	PPI
MS.gene69586	MS.gene000640	PPI
MS.gene042027	MS.gene000640	PPI
MS.gene85308	MS.gene000640	PPI
MS.gene69490	MS.gene000640	PPI
MS.gene043140	MS.gene000640	PPI
MS.gene015248	MS.gene000640	PPI
MS.gene012889	MS.gene000640	PPI
MS.gene80362	MS.gene000640	PPI
MS.gene44083	MS.gene000640	PPI
MS.gene37537	MS.gene000640	PPI
MS.gene013193	MS.gene000640	PPI
MS.gene04970	MS.gene000640	PPI
MS.gene08106	MS.gene000640	PPI
MS.gene51799	MS.gene000640	PPI
MS.gene58419	MS.gene000640	PPI
MS.gene000640	MS.gene44144	PPI
MS.gene027994	MS.gene000640	PPI
MS.gene068629	MS.gene000640	PPI
MS.gene96163	MS.gene000640	PPI
MS.gene08713	MS.gene000640	PPI
MS.gene65474	MS.gene000640	PPI
MS.gene23521	MS.gene000640	PPI
MS.gene29406	MS.gene000640	PPI
MS.gene86275	MS.gene000640	PPI
MS.gene98127	MS.gene000640	PPI
MS.gene03149	MS.gene000640	PPI
MS.gene87529	MS.gene000640	PPI
MS.gene024405	MS.gene000640	PPI
MS.gene04134	MS.gene000640	PPI
MS.gene062067	MS.gene000640	PPI
MS.gene37955	MS.gene000640	PPI
MS.gene57044	MS.gene000640	PPI
MS.gene054508	MS.gene000640	PPI
MS.gene89815	MS.gene000640	PPI
MS.gene028714	MS.gene000640	PPI
MS.gene017586	MS.gene000640	PPI
MS.gene038676	MS.gene000640	PPI
MS.gene98756	MS.gene000640	PPI
MS.gene71637	MS.gene000640	PPI
MS.gene042651	MS.gene000640	PPI
MS.gene000640	MS.gene018474	PPI
MS.gene000640	MS.gene86868	PPI
MS.gene98467	MS.gene000640	PPI
MS.gene037049	MS.gene000640	PPI
MS.gene034643	MS.gene000640	PPI
MS.gene044729	MS.gene000640	PPI
MS.gene037634	MS.gene000640	PPI
MS.gene000379	MS.gene000640	PPI
MS.gene24070	MS.gene000640	PPI
MS.gene60266	MS.gene000640	PPI
MS.gene071784	MS.gene000640	PPI
MS.gene027169	MS.gene000640	PPI
MS.gene061579	MS.gene000640	PPI
MS.gene039692	MS.gene000640	PPI
MS.gene76420	MS.gene000640	PPI
MS.gene071320	MS.gene000640	PPI
MS.gene022929	MS.gene000640	PPI
MS.gene000640	MS.gene61499	PPI
MS.gene42903	MS.gene000640	PPI
MS.gene81712	MS.gene000640	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000640.t1	MTR_3g053920	48.551	276	43	4	1	227	55	280	8.23e-69	215
MS.gene000640.t1	MTR_8g463110	77.000	100	23	0	190	289	21	120	7.00e-33	127
MS.gene000640.t1	MTR_4g103530	35.025	197	84	8	1	190	1	160	5.94e-17	77.8
MS.gene000640.t1	MTR_2g061500	84.211	38	6	0	1	38	55	92	4.67e-15	71.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 83 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAATTTGCAGCAGTAGTAAA+AGG	0.292724	5.1:-19315076	None:intergenic
GATGAGGGAAATACAGTAAT+AGG	0.305593	5.1:+19315187	MS.gene000640:CDS
CTGACGACGGAGTCCGTAAC+TGG	0.308670	5.1:+19314684	MS.gene000640:CDS
CACCTTCAACAGTTGCATTT+CGG	0.328086	5.1:-19314829	None:intergenic
CTGGTGCTTGGACTGTCTGC+TGG	0.392114	5.1:+19315046	MS.gene000640:CDS
CCTACTTGGCCTCTGGTGCT+TGG	0.404644	5.1:+19315034	MS.gene000640:CDS
TAACTTTAAATATGAAAAGC+TGG	0.406564	5.1:+19314589	MS.gene000640:CDS
GACACGCCACTCTTGTTTCA+AGG	0.413036	5.1:-19314537	None:intergenic
GCCAAAGCAAAACAAGGTTC+TGG	0.414080	5.1:+19314941	MS.gene000640:CDS
CTCTTACCGTCCTCTTCAAC+AGG	0.419316	5.1:+19314989	MS.gene000640:CDS
GTTGCTGCTGCTGCCTCTGT+TGG	0.421080	5.1:+19314806	MS.gene000640:CDS
TCCTCTTCAACAGGTAACTT+AGG	0.427895	5.1:+19314998	MS.gene000640:CDS
GCAAATTCTCAAGAGGAAAT+CGG	0.445460	5.1:+19315091	MS.gene000640:CDS
GCAGCAGTAGTAAAAGGAAA+TGG	0.452091	5.1:-19315070	None:intergenic
CGCGGCACAACTACGGGAAT+AGG	0.452197	5.1:-19314773	None:intergenic
GTGATCGTGACCCTCTTGCC+TGG	0.454623	5.1:-19314197	None:intergenic
TCTACTTCCTACTTGGCCTC+TGG	0.463232	5.1:+19315027	MS.gene000640:CDS
GGTTCTGGTGCGAACTCTCC+AGG	0.468743	5.1:+19314956	MS.gene000640:CDS
GGTCCAATCTACTTCCTACT+TGG	0.469116	5.1:+19315020	MS.gene000640:CDS
AGGCCAAGTAGGAAGTAGAT+TGG	0.472065	5.1:-19315023	None:intergenic
TGTGTGGCGCGGCACAACTA+CGG	0.473973	5.1:-19314780	None:intergenic
TGAGATAAGCACCCCAGTTA+CGG	0.477173	5.1:-19314697	None:intergenic
ATGTCTCGCGCATGGAAGCC+AGG	0.480456	5.1:+19314179	MS.gene000640:CDS
TCGGTACTGCTGTAACCAAC+AGG	0.484719	5.1:+19314738	MS.gene000640:CDS
GCAGACAGTCCAAGCACCAG+AGG	0.486586	5.1:-19315043	None:intergenic
TGTCACAGGTGTGGTGTCCT+CGG	0.489686	5.1:+19314620	MS.gene000640:CDS
TGTAACCAACAGGTGGATAC+AGG	0.500442	5.1:+19314748	MS.gene000640:CDS
CCTCTTCAACAGGTAACTTA+GGG	0.502533	5.1:+19314999	MS.gene000640:CDS
GTGTGGCGCGGCACAACTAC+GGG	0.503725	5.1:-19314779	None:intergenic
CCAAGCACCAGAGGCCAAGT+AGG	0.508320	5.1:-19315034	None:intergenic
AACTTTAAATATGAAAAGCT+GGG	0.509761	5.1:+19314590	MS.gene000640:CDS
TAGGAACAAATGTTAGTCAT+GGG	0.515296	5.1:+19315206	MS.gene000640:CDS
ACATACGGACAAGGTGTGTC+CGG	0.515722	5.1:+19314643	MS.gene000640:CDS
ACCATCCTGTCATTGAAGCT+TGG	0.516139	5.1:-19314860	None:intergenic
GTACCGATACGTTGCAAAGT+AGG	0.518389	5.1:-19314722	None:intergenic
GGTAAGAGATGTACCATGCC+TGG	0.518626	5.1:-19314974	None:intergenic
TGCAACTGTTGAAGGTGTAC+TGG	0.530799	5.1:+19314835	MS.gene000640:CDS
GAAGAAAATAAAGAGGATGC+TGG	0.533193	5.1:+19315123	MS.gene000640:CDS
TGGTTCCAAGCTTCAATGAC+AGG	0.533487	5.1:+19314855	MS.gene000640:CDS
GCTTTCAATTCAAACAGCTC+CGG	0.535106	5.1:-19314662	None:intergenic
GCAGCAACATTTGTGTGGCG+CGG	0.535223	5.1:-19314791	None:intergenic
GCAGCACGTGTTCTCGATGA+AGG	0.536950	5.1:+19314269	MS.gene000640:CDS
GGCCGAAATGCAACTGTTGA+AGG	0.538531	5.1:+19314827	MS.gene000640:CDS
GTGTGGTGTCCTCGGACATA+CGG	0.541284	5.1:+19314628	MS.gene000640:CDS
ATAGGAACAAATGTTAGTCA+TGG	0.543713	5.1:+19315205	MS.gene000640:CDS
ATGAATTGTCACCATTCTGA+AGG	0.545818	5.1:-19315147	None:intergenic
CCCTAAGTTACCTGTTGAAG+AGG	0.548014	5.1:-19314999	None:intergenic
TACTGCTGCAAATTCTCAAG+AGG	0.568886	5.1:+19315084	MS.gene000640:CDS
CTGAATTGAAGAAAATAAAG+AGG	0.569177	5.1:+19315116	MS.gene000640:CDS
ACACGCCACTCTTGTTTCAA+GGG	0.575839	5.1:-19314536	None:intergenic
AAGCCTACTTTGCAACGTAT+CGG	0.575843	5.1:+19314719	MS.gene000640:CDS
CAAAAGTACCATTGTTAGCA+CGG	0.580700	5.1:-19314559	None:intergenic
TGGTGCGAACTCTCCAGGCA+TGG	0.581722	5.1:+19314961	MS.gene000640:CDS
CCAAAAGTAGGTCAAGGCAT+AGG	0.584813	5.1:+19314422	MS.gene000640:intron
TGACGACGGAGTCCGTAACT+GGG	0.587050	5.1:+19314685	MS.gene000640:CDS
AGGATGCTGGCCCTTCAGAA+TGG	0.591615	5.1:+19315136	MS.gene000640:CDS
AGGATCCTGTATCCACCTGT+TGG	0.592031	5.1:-19314753	None:intergenic
CGCATGGAAGCCAGGCAAGA+GGG	0.592982	5.1:+19314187	MS.gene000640:CDS
AAGTAGGTCAAGGCATAGGA+AGG	0.603236	5.1:+19314426	MS.gene000640:intron
CACGTGCTGCATCAACTTTG+TGG	0.609342	5.1:-19314256	None:intergenic
TCCAAGCTTCAATGACAGGA+TGG	0.614971	5.1:+19314859	MS.gene000640:CDS
GTGAGCGTATGTCTCGCGCA+TGG	0.619522	5.1:+19314171	None:intergenic
AGGAACAAATGTTAGTCATG+GGG	0.621613	5.1:+19315207	MS.gene000640:CDS
GCGCATGGAAGCCAGGCAAG+AGG	0.622091	5.1:+19314186	MS.gene000640:CDS
GGAACAAATGTTAGTCATGG+GGG	0.626566	5.1:+19315208	MS.gene000640:CDS
TTTGTTGCTGATGAGGATGA+GGG	0.628969	5.1:+19315172	MS.gene000640:CDS
TGGCGTGTCCGTGCTAACAA+TGG	0.633755	5.1:+19314551	MS.gene000640:CDS
TGAATTGTCACCATTCTGAA+GGG	0.638224	5.1:-19315146	None:intergenic
CAGCAGCAGCAACATTTGTG+TGG	0.642797	5.1:-19314796	None:intergenic
AAGTTACCTGTTGAAGAGGA+CGG	0.645741	5.1:-19314995	None:intergenic
CTGTTCCCTTGAAACAAGAG+TGG	0.655372	5.1:+19314531	MS.gene000640:CDS
TAGGCTGCCAAAGCAAAACA+AGG	0.657259	5.1:+19314935	MS.gene000640:intron
TGTCCTCGGACATACGGACA+AGG	0.659763	5.1:+19314634	MS.gene000640:CDS
ACACCTTGTCCGTATGTCCG+AGG	0.663830	5.1:-19314637	None:intergenic
GACGACGGAGTCCGTAACTG+GGG	0.666040	5.1:+19314686	MS.gene000640:CDS
GAACAAATGTTAGTCATGGG+GGG	0.666834	5.1:+19315209	MS.gene000640:CDS
TTTGAATTGAAAGCTGACGA+CGG	0.669935	5.1:+19314671	MS.gene000640:CDS
GTGCTGCATCAACTTTGTGG+TGG	0.674580	5.1:-19314253	None:intergenic
GGTCACGATCACTAAGTCTG+TGG	0.683213	5.1:+19314208	MS.gene000640:CDS
GTACTGCTGTAACCAACAGG+TGG	0.687537	5.1:+19314741	MS.gene000640:CDS
AGTTGCATTTCGGCCAACAG+AGG	0.693072	5.1:-19314819	None:intergenic
TGCGACACAGATACGCATGT+AGG	0.697372	5.1:-19314498	None:intergenic
AGATACGCATGTAGGAACTG+TGG	0.699175	5.1:-19314490	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AACTTTAAATATGAAAAGCT+GGG	+	chr5.1:19314590-19314609	MS.gene000640:CDS	20.0%
!!	TAACTTTAAATATGAAAAGC+TGG	+	chr5.1:19314589-19314608	MS.gene000640:CDS	20.0%
!	CTGAATTGAAGAAAATAAAG+AGG	+	chr5.1:19315116-19315135	MS.gene000640:CDS	25.0%
	AAAAATCTATGTGGTTCAGA+GGG	-	chr5.1:19314364-19314383	None:intergenic	30.0%
	ATAGGAACAAATGTTAGTCA+TGG	+	chr5.1:19315205-19315224	MS.gene000640:CDS	30.0%
	TAGGAACAAATGTTAGTCAT+GGG	+	chr5.1:19315206-19315225	MS.gene000640:CDS	30.0%
	TGATAACTTTCATATCGTCA+TGG	+	chr5.1:19314304-19314323	MS.gene000640:intron	30.0%
	TTTGGCTGGATAAATCAAAA+GGG	-	chr5.1:19314407-19314426	None:intergenic	30.0%
!!	GATTTTTGGATCCAAGTTTA+TGG	+	chr5.1:19314377-19314396	MS.gene000640:intron	30.0%
!!	TTTTGGCTGGATAAATCAAA+AGG	-	chr5.1:19314408-19314427	None:intergenic	30.0%
	AAAAGGGTAAGCCATAAACT+TGG	-	chr5.1:19314391-19314410	None:intergenic	35.0%
	AGGAACAAATGTTAGTCATG+GGG	+	chr5.1:19315207-19315226	MS.gene000640:CDS	35.0%
	ATCACTGCAATCAAACATGA+TGG	+	chr5.1:19314899-19314918	MS.gene000640:intron	35.0%
	ATGAATTGTCACCATTCTGA+AGG	-	chr5.1:19315150-19315169	None:intergenic	35.0%
	CAAAAATCTATGTGGTTCAG+AGG	-	chr5.1:19314365-19314384	None:intergenic	35.0%
	CATGTTTGATTGCAGTGATT+TGG	-	chr5.1:19314898-19314917	None:intergenic	35.0%
	CTTGGATCCAAAAATCTATG+TGG	-	chr5.1:19314373-19314392	None:intergenic	35.0%
	GAAGAAAATAAAGAGGATGC+TGG	+	chr5.1:19315123-19315142	MS.gene000640:CDS	35.0%
	GAATTTGCAGCAGTAGTAAA+AGG	-	chr5.1:19315079-19315098	None:intergenic	35.0%
	GATGAGGGAAATACAGTAAT+AGG	+	chr5.1:19315187-19315206	MS.gene000640:CDS	35.0%
	GCAAATTCTCAAGAGGAAAT+CGG	+	chr5.1:19315091-19315110	MS.gene000640:CDS	35.0%
	TGAATTGTCACCATTCTGAA+GGG	-	chr5.1:19315149-19315168	None:intergenic	35.0%
	TGATTTATCCAGCCAAAAGT+AGG	+	chr5.1:19314410-19314429	MS.gene000640:intron	35.0%
	TTTGAATTGAAAGCTGACGA+CGG	+	chr5.1:19314671-19314690	MS.gene000640:CDS	35.0%
!	CTCTGAACCACATAGATTTT+TGG	+	chr5.1:19314363-19314382	MS.gene000640:intron	35.0%
!	TTCATCTTTTGTTGCTGATG+AGG	+	chr5.1:19315165-19315184	MS.gene000640:CDS	35.0%
!!	CAAAAGTACCATTGTTAGCA+CGG	-	chr5.1:19314562-19314581	None:intergenic	35.0%
	AAGCCTACTTTGCAACGTAT+CGG	+	chr5.1:19314719-19314738	MS.gene000640:CDS	40.0%
	AAGTTACCTGTTGAAGAGGA+CGG	-	chr5.1:19314998-19315017	None:intergenic	40.0%
	ACTTTCATATCGTCATGGAC+CGG	+	chr5.1:19314309-19314328	MS.gene000640:intron	40.0%
	CACCTTCAACAGTTGCATTT+CGG	-	chr5.1:19314832-19314851	None:intergenic	40.0%
	CCTCTTCAACAGGTAACTTA+GGG	+	chr5.1:19314999-19315018	MS.gene000640:CDS	40.0%
	GAACAAATGTTAGTCATGGG+GGG	+	chr5.1:19315209-19315228	MS.gene000640:CDS	40.0%
	GCAGCAGTAGTAAAAGGAAA+TGG	-	chr5.1:19315073-19315092	None:intergenic	40.0%
	GCTTTCAATTCAAACAGCTC+CGG	-	chr5.1:19314665-19314684	None:intergenic	40.0%
	GGAACAAATGTTAGTCATGG+GGG	+	chr5.1:19315208-19315227	MS.gene000640:CDS	40.0%
	TACTGCTGCAAATTCTCAAG+AGG	+	chr5.1:19315084-19315103	MS.gene000640:CDS	40.0%
	TCCTCTTCAACAGGTAACTT+AGG	+	chr5.1:19314998-19315017	MS.gene000640:CDS	40.0%
	TTTGTTGCTGATGAGGATGA+GGG	+	chr5.1:19315172-19315191	MS.gene000640:CDS	40.0%
!	CAAGGCATAGGAAGGTTTTT+AGG	+	chr5.1:19314434-19314453	MS.gene000640:CDS	40.0%
!	TTTTGTTGCTGATGAGGATG+AGG	+	chr5.1:19315171-19315190	MS.gene000640:CDS	40.0%
!!	GGTACTTTTAAGTCGTACGA+TGG	+	chr5.1:19314455-19314474	MS.gene000640:CDS	40.0%
!!!	ACCAGAACCTTGTTTTGCTT+TGG	-	chr5.1:19314945-19314964	None:intergenic	40.0%
	AAAAGAGGGAACGACATCAG+GGG	-	chr5.1:19314339-19314358	None:intergenic	45.0%
	AAGTAGGTCAAGGCATAGGA+AGG	+	chr5.1:19314426-19314445	MS.gene000640:intron	45.0%
	ACAAAAGAGGGAACGACATC+AGG	-	chr5.1:19314341-19314360	None:intergenic	45.0%
	ACCATCCTGTCATTGAAGCT+TGG	-	chr5.1:19314863-19314882	None:intergenic	45.0%
	AGATACGCATGTAGGAACTG+TGG	-	chr5.1:19314493-19314512	None:intergenic	45.0%
	AGGCCAAGTAGGAAGTAGAT+TGG	-	chr5.1:19315026-19315045	None:intergenic	45.0%
	ATCCAGCCAAAAGTAGGTCA+AGG	+	chr5.1:19314416-19314435	MS.gene000640:intron	45.0%
	CAAAAGAGGGAACGACATCA+GGG	-	chr5.1:19314340-19314359	None:intergenic	45.0%
	CCAAAAGTAGGTCAAGGCAT+AGG	+	chr5.1:19314422-19314441	MS.gene000640:intron	45.0%
	CCCTAAGTTACCTGTTGAAG+AGG	-	chr5.1:19315002-19315021	None:intergenic	45.0%
	CTGTTCCCTTGAAACAAGAG+TGG	+	chr5.1:19314531-19314550	MS.gene000640:CDS	45.0%
	GCCAAAGCAAAACAAGGTTC+TGG	+	chr5.1:19314941-19314960	MS.gene000640:CDS	45.0%
	GGTCCAATCTACTTCCTACT+TGG	+	chr5.1:19315020-19315039	MS.gene000640:CDS	45.0%
	TAGGCTGCCAAAGCAAAACA+AGG	+	chr5.1:19314935-19314954	MS.gene000640:intron	45.0%
	TCCAAGCTTCAATGACAGGA+TGG	+	chr5.1:19314859-19314878	MS.gene000640:CDS	45.0%
	TGAGATAAGCACCCCAGTTA+CGG	-	chr5.1:19314700-19314719	None:intergenic	45.0%
	TGGTTCAGAGGGACAAAAGA+GGG	-	chr5.1:19314353-19314372	None:intergenic	45.0%
	TGGTTCCAAGCTTCAATGAC+AGG	+	chr5.1:19314855-19314874	MS.gene000640:CDS	45.0%
	TGTAACCAACAGGTGGATAC+AGG	+	chr5.1:19314748-19314767	MS.gene000640:CDS	45.0%
!	ACACGCCACTCTTGTTTCAA+GGG	-	chr5.1:19314539-19314558	None:intergenic	45.0%
!	CCTATGCCTTGACCTACTTT+TGG	-	chr5.1:19314425-19314444	None:intergenic	45.0%
!	GTACCGATACGTTGCAAAGT+AGG	-	chr5.1:19314725-19314744	None:intergenic	45.0%
!	GTGTTCTCGATGAAGGTATG+TGG	+	chr5.1:19314276-19314295	MS.gene000640:intron	45.0%
!	TGATGGTCTTGCTGCTCAAT+AGG	+	chr5.1:19314916-19314935	MS.gene000640:intron	45.0%
!	TGCAACTGTTGAAGGTGTAC+TGG	+	chr5.1:19314835-19314854	MS.gene000640:CDS	45.0%
!!!	AGCTGGGTGTTTTTTGTCAC+AGG	+	chr5.1:19314606-19314625	MS.gene000640:CDS	45.0%
!!!	GGTGTTTTTTGTCACAGGTG+TGG	+	chr5.1:19314611-19314630	MS.gene000640:CDS	45.0%
	ACATACGGACAAGGTGTGTC+CGG	+	chr5.1:19314643-19314662	MS.gene000640:CDS	50.0%
	AGGATCCTGTATCCACCTGT+TGG	-	chr5.1:19314756-19314775	None:intergenic	50.0%
	AGTTGCATTTCGGCCAACAG+AGG	-	chr5.1:19314822-19314841	None:intergenic	50.0%
	CACGTGCTGCATCAACTTTG+TGG	-	chr5.1:19314259-19314278	None:intergenic	50.0%
	CAGCAGCAGCAACATTTGTG+TGG	-	chr5.1:19314799-19314818	None:intergenic	50.0%
	CTCTTACCGTCCTCTTCAAC+AGG	+	chr5.1:19314989-19315008	MS.gene000640:CDS	50.0%
	GAACGACATCAGGGGAAATC+CGG	-	chr5.1:19314331-19314350	None:intergenic	50.0%
	GGCCGAAATGCAACTGTTGA+AGG	+	chr5.1:19314827-19314846	MS.gene000640:CDS	50.0%
	GGTAAGAGATGTACCATGCC+TGG	-	chr5.1:19314977-19314996	None:intergenic	50.0%
	GGTCACGATCACTAAGTCTG+TGG	+	chr5.1:19314208-19314227	MS.gene000640:CDS	50.0%
	GTACTGCTGTAACCAACAGG+TGG	+	chr5.1:19314741-19314760	MS.gene000640:CDS	50.0%
	GTGCTGCATCAACTTTGTGG+TGG	-	chr5.1:19314256-19314275	None:intergenic	50.0%
	GTGGTTCAGAGGGACAAAAG+AGG	-	chr5.1:19314354-19314373	None:intergenic	50.0%
	TCGGTACTGCTGTAACCAAC+AGG	+	chr5.1:19314738-19314757	MS.gene000640:CDS	50.0%
	TCTACTTCCTACTTGGCCTC+TGG	+	chr5.1:19315027-19315046	MS.gene000640:CDS	50.0%
	TGCGACACAGATACGCATGT+AGG	-	chr5.1:19314501-19314520	None:intergenic	50.0%
!	GACACGCCACTCTTGTTTCA+AGG	-	chr5.1:19314540-19314559	None:intergenic	50.0%
!!	TGCCTTGACCTACTTTTGGC+TGG	-	chr5.1:19314421-19314440	None:intergenic	50.0%
	ACACCTTGTCCGTATGTCCG+AGG	-	chr5.1:19314640-19314659	None:intergenic	55.0%
	AGGATGCTGGCCCTTCAGAA+TGG	+	chr5.1:19315136-19315155	MS.gene000640:CDS	55.0%
	GCAGCAACATTTGTGTGGCG+CGG	-	chr5.1:19314794-19314813	None:intergenic	55.0%
	GCAGCACGTGTTCTCGATGA+AGG	+	chr5.1:19314269-19314288	MS.gene000640:CDS	55.0%
	GTGTGGTGTCCTCGGACATA+CGG	+	chr5.1:19314628-19314647	MS.gene000640:CDS	55.0%
	TGACGACGGAGTCCGTAACT+GGG	+	chr5.1:19314685-19314704	MS.gene000640:CDS	55.0%
	TGGCGTGTCCGTGCTAACAA+TGG	+	chr5.1:19314551-19314570	MS.gene000640:CDS	55.0%
	TGTCACAGGTGTGGTGTCCT+CGG	+	chr5.1:19314620-19314639	MS.gene000640:CDS	55.0%
	TGTCCTCGGACATACGGACA+AGG	+	chr5.1:19314634-19314653	MS.gene000640:CDS	55.0%
	ATGTCTCGCGCATGGAAGCC+AGG	+	chr5.1:19314179-19314198	MS.gene000640:CDS	60.0%
	CCAAGCACCAGAGGCCAAGT+AGG	-	chr5.1:19315037-19315056	None:intergenic	60.0%
	CCTACTTGGCCTCTGGTGCT+TGG	+	chr5.1:19315034-19315053	MS.gene000640:CDS	60.0%
	CGCATGGAAGCCAGGCAAGA+GGG	+	chr5.1:19314187-19314206	MS.gene000640:CDS	60.0%
	CGCGGCACAACTACGGGAAT+AGG	-	chr5.1:19314776-19314795	None:intergenic	60.0%
	CTGACGACGGAGTCCGTAAC+TGG	+	chr5.1:19314684-19314703	MS.gene000640:CDS	60.0%
	CTGGTGCTTGGACTGTCTGC+TGG	+	chr5.1:19315046-19315065	MS.gene000640:CDS	60.0%
	GACGACGGAGTCCGTAACTG+GGG	+	chr5.1:19314686-19314705	MS.gene000640:CDS	60.0%
	GCAGACAGTCCAAGCACCAG+AGG	-	chr5.1:19315046-19315065	None:intergenic	60.0%
	GTGATCGTGACCCTCTTGCC+TGG	-	chr5.1:19314200-19314219	None:intergenic	60.0%
	GTTGCTGCTGCTGCCTCTGT+TGG	+	chr5.1:19314806-19314825	MS.gene000640:CDS	60.0%
	TGGTGCGAACTCTCCAGGCA+TGG	+	chr5.1:19314961-19314980	MS.gene000640:CDS	60.0%
	TGTGTGGCGCGGCACAACTA+CGG	-	chr5.1:19314783-19314802	None:intergenic	60.0%
!	GGTTCTGGTGCGAACTCTCC+AGG	+	chr5.1:19314956-19314975	MS.gene000640:CDS	60.0%
	GCGCATGGAAGCCAGGCAAG+AGG	+	chr5.1:19314186-19314205	MS.gene000640:CDS	65.0%
	GTGTGGCGCGGCACAACTAC+GGG	-	chr5.1:19314782-19314801	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	19314179	19315243	19314179	ID=MS.gene000640
chr5.1	mRNA	19314179	19315243	19314179	ID=MS.gene000640.t1;Parent=MS.gene000640
chr5.1	exon	19314179	19314290	19314179	ID=MS.gene000640.t1.exon1;Parent=MS.gene000640.t1
chr5.1	CDS	19314179	19314290	19314179	ID=cds.MS.gene000640.t1;Parent=MS.gene000640.t1
chr5.1	exon	19314432	19314886	19314432	ID=MS.gene000640.t1.exon2;Parent=MS.gene000640.t1
chr5.1	CDS	19314432	19314886	19314432	ID=cds.MS.gene000640.t1;Parent=MS.gene000640.t1
chr5.1	exon	19314938	19315243	19314938	ID=MS.gene000640.t1.exon3;Parent=MS.gene000640.t1
chr5.1	CDS	19314938	19315243	19314938	ID=cds.MS.gene000640.t1;Parent=MS.gene000640.t1

Gene Sequence

>MS.gene000640

ATGTCTCGCGCATGGAAGCCAGGCAAGAGGGTCACGATCACTAAGTCTGTGGTTAACTGCTATCTGTTTCAGTTCCACCACAAAGTTGATGCAGCACGTGTTCTCGATGAAGGTATGTGGCTTTATGATAACTTTCATATCGTCATGGACCGGATTTCCCCTGATGTCGTTCCCTCTTTTGTCCCTCTGAACCACATAGATTTTTGGATCCAAGTTTATGGCTTACCCTTTTGATTTATCCAGCCAAAAGTAGGTCAAGGCATAGGAAGGTTTTTAGGTACTTTTAAGTCGTACGATGGTCGTAATTCAATCCACAGTTCCTACATGCGTATCTGTGTCGCAATTGATGTGACTGTTCCCTTGAAACAAGAGTGGCGTGTCCGTGCTAACAATGGTACTTTTGTTACTGTTAACTTTAAATATGAAAAGCTGGGTGTTTTTTGTCACAGGTGTGGTGTCCTCGGACATACGGACAAGGTGTGTCCGGAGCTGTTTGAATTGAAAGCTGACGACGGAGTCCGTAACTGGGGTGCTTATCTCAAGCCTACTTTGCAACGTATCGGTACTGCTGTAACCAACAGGTGGATACAGGATCCTATTCCCGTAGTTGTGCCGCGCCACACAAATGTTGCTGCTGCTGCCTCTGTTGGCCGAAATGCAACTGTTGAAGGTGTACTGGTTCCAAGCTTCAATGACAGGATGGTAGCTGTACAGAACCAAATCACTGCAATCAAACATGATGGTCTTGCTGCTCAATAGGCTGCCAAAGCAAAACAAGGTTCTGGTGCGAACTCTCCAGGCATGGTACATCTCTTACCGTCCTCTTCAACAGGTAACTTAGGGTCCAATCTACTTCCTACTTGGCCTCTGGTGCTTGGACTGTCTGCTGGTCCATTTCCTTTTACTACTGCTGCAAATTCTCAAGAGGAAATCGGCTCTGAATTGAAGAAAATAAAGAGGATGCTGGCCCTTCAGAATGGTGACAATTCATCTTTTGTTGCTGATGAGGATGAGGGAAATACAGTAATAGGAACAAATGTTAGTCATGGGGGGATGATGATATGA

Protein sequence

>MS.gene000640.t1

MSRAWKPGKRVTITKSVVNCYLFQFHHKVDAARVLDEGQGIGRFLGTFKSYDGRNSIHSSYMRICVAIDVTVPLKQEWRVRANNGTFVTVNFKYEKLGVFCHRCGVLGHTDKVCPELFELKADDGVRNWGAYLKPTLQRIGTAVTNRWIQDPIPVVVPRHTNVAAAASVGRNATVEGVLVPSFNDRMVAAAKAKQGSGANSPGMVHLLPSSSTGNLGSNLLPTWPLVLGLSAGPFPFTTAANSQEEIGSELKKIKRMLALQNGDNSSFVADEDEGNTVIGTNVSHGGMMI