AlfalfaGEDB | MS.gene064966

M. sativa cultivar XinJiangDaYe / MS.gene064966

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064966.t1	PNY16581.1	82.5	228	40	0	1	228	1	228	1.60E-111	412.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064966.t1	A0A2K3PMS8	82.5	228	40	0	1	228	1	228	1.2e-111	412.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene064966	MS.gene019001	PPI
MS.gene064966	MS.gene021965	PPI
MS.gene064966	MS.gene53904	PPI
MS.gene064966	MS.gene24070	PPI
MS.gene064966	MS.gene83772	PPI
MS.gene064966	MS.gene73261	PPI
MS.gene064966	MS.gene059736	PPI
MS.gene064966	MS.gene85538	PPI
MS.gene021968	MS.gene064966	PPI
MS.gene064966	MS.gene025675	PPI
MS.gene064966	MS.gene055546	PPI
MS.gene09589	MS.gene064966	PPI
MS.gene064966	MS.gene71637	PPI
MS.gene064966	MS.gene55209	PPI
MS.gene064966	MS.gene96174	PPI
MS.gene064966	MS.gene073573	PPI
MS.gene43393	MS.gene064966	PPI
MS.gene064966	MS.gene28718	PPI
MS.gene064966	MS.gene63743	PPI
MS.gene064966	MS.gene068629	PPI
MS.gene064966	MS.gene009781	PPI
MS.gene064966	MS.gene047954	PPI
MS.gene04749	MS.gene064966	PPI
MS.gene064966	MS.gene048342	PPI
MS.gene05436	MS.gene064966	PPI
MS.gene064966	MS.gene21066	PPI
MS.gene064966	MS.gene013890	PPI
MS.gene064966	MS.gene98127	PPI
MS.gene064966	MS.gene073582	PPI
MS.gene064966	MS.gene003264	PPI
MS.gene42903	MS.gene064966	PPI
MS.gene055683	MS.gene064966	PPI
MS.gene064966	MS.gene86849	PPI
MS.gene064966	MS.gene048706	PPI
MS.gene064966	MS.gene45083	PPI
MS.gene064966	MS.gene049561	PPI
MS.gene048747	MS.gene064966	PPI
MS.gene064966	MS.gene008658	PPI
MS.gene064966	MS.gene47792	PPI
MS.gene064966	MS.gene80362	PPI
MS.gene064966	MS.gene49057	PPI
MS.gene064966	MS.gene89815	PPI
MS.gene064966	MS.gene43393	PPI
MS.gene064966	MS.gene048088	PPI
MS.gene064966	MS.gene86868	PPI
MS.gene94654	MS.gene064966	PPI
MS.gene064966	MS.gene058889	PPI
MS.gene031967	MS.gene064966	PPI
MS.gene064966	MS.gene034643	PPI
MS.gene064966	MS.gene60059	PPI
MS.gene063214	MS.gene064966	PPI
MS.gene97410	MS.gene064966	PPI
MS.gene83772	MS.gene064966	PPI
MS.gene32415	MS.gene064966	PPI
MS.gene035626	MS.gene064966	PPI
MS.gene064966	MS.gene012889	PPI
MS.gene064966	MS.gene34463	PPI
MS.gene064966	MS.gene038757	PPI
MS.gene064966	MS.gene000506	PPI
MS.gene63762	MS.gene064966	PPI
MS.gene30208	MS.gene064966	PPI
MS.gene69495	MS.gene064966	PPI
MS.gene064966	MS.gene51804	PPI
MS.gene064966	MS.gene013644	PPI
MS.gene064966	MS.gene54051	PPI
MS.gene030090	MS.gene064966	PPI
MS.gene95561	MS.gene064966	PPI
MS.gene018196	MS.gene064966	PPI
MS.gene065627	MS.gene064966	PPI
MS.gene064966	MS.gene010890	PPI
MS.gene57019	MS.gene064966	PPI
MS.gene064966	MS.gene061579	PPI
MS.gene038098	MS.gene064966	PPI
MS.gene064966	MS.gene04970	PPI
MS.gene60059	MS.gene064966	PPI
MS.gene064966	MS.gene26038	PPI
MS.gene064966	MS.gene060788	PPI
MS.gene064966	MS.gene43924	PPI
MS.gene064966	MS.gene41643	PPI
MS.gene064966	MS.gene008861	PPI
MS.gene008275	MS.gene064966	PPI
MS.gene064966	MS.gene027994	PPI
MS.gene064966	MS.gene022929	PPI
MS.gene064966	MS.gene85295	PPI
MS.gene064966	MS.gene26433	PPI
MS.gene064966	MS.gene007022	PPI
MS.gene064966	MS.gene69584	PPI
MS.gene064966	MS.gene52349	PPI
MS.gene048072	MS.gene064966	PPI
MS.gene064966	MS.gene021647	PPI
MS.gene064966	MS.gene46353	PPI
MS.gene064966	MS.gene65471	PPI
MS.gene064966	MS.gene071784	PPI
MS.gene064966	MS.gene069624	PPI
MS.gene064966	MS.gene060386	PPI
MS.gene064966	MS.gene83979	PPI
MS.gene064966	MS.gene61651	PPI
MS.gene036423	MS.gene064966	PPI
MS.gene064966	MS.gene63762	PPI
MS.gene064966	MS.gene016719	PPI
MS.gene064966	MS.gene048072	PPI
MS.gene064966	MS.gene028714	PPI
MS.gene064966	MS.gene88393	PPI
MS.gene064966	MS.gene042027	PPI
MS.gene064966	MS.gene054508	PPI
MS.gene064966	MS.gene69586	PPI
MS.gene064966	MS.gene053373	PPI
MS.gene66651	MS.gene064966	PPI
MS.gene96109	MS.gene064966	PPI
MS.gene064966	MS.gene35175	PPI
MS.gene064966	MS.gene63150	PPI
MS.gene051449	MS.gene064966	PPI
MS.gene064966	MS.gene03936	PPI
MS.gene064966	MS.gene44083	PPI
MS.gene064966	MS.gene000629	PPI
MS.gene064966	MS.gene072852	PPI
MS.gene064966	MS.gene65474	PPI
MS.gene064966	MS.gene08708	PPI
MS.gene064966	MS.gene04134	PPI
MS.gene064966	MS.gene06164	PPI
MS.gene064966	MS.gene50392	PPI
MS.gene064966	MS.gene86275	PPI
MS.gene064966	MS.gene22730	PPI
MS.gene064966	MS.gene032299	PPI
MS.gene064966	MS.gene017586	PPI
MS.gene30136	MS.gene064966	PPI
MS.gene064966	MS.gene038098	PPI
MS.gene064966	MS.gene037634	PPI
MS.gene064966	MS.gene057828	PPI
MS.gene064966	MS.gene071320	PPI
MS.gene064966	MS.gene08606	PPI
MS.gene064966	MS.gene012019	PPI
MS.gene016102	MS.gene064966	PPI
MS.gene46941	MS.gene064966	PPI
MS.gene96541	MS.gene064966	PPI
MS.gene064966	MS.gene000640	PPI
MS.gene064966	MS.gene061850	PPI
MS.gene064966	MS.gene98756	PPI
MS.gene064966	MS.gene39956	PPI
MS.gene47792	MS.gene064966	PPI
MS.gene46948	MS.gene064966	PPI
MS.gene025873	MS.gene064966	PPI
MS.gene012019	MS.gene064966	PPI
MS.gene064966	MS.gene028716	PPI
MS.gene064966	MS.gene74639	PPI
MS.gene064966	MS.gene071133	PPI
MS.gene064966	MS.gene64130	PPI
MS.gene064966	MS.gene60266	PPI
MS.gene064966	MS.gene98467	PPI
MS.gene064966	MS.gene62249	PPI
MS.gene064966	MS.gene024522	PPI
MS.gene05358	MS.gene064966	PPI
MS.gene064966	MS.gene22111	PPI
MS.gene064966	MS.gene046089	PPI
MS.gene064966	MS.gene049610	PPI
MS.gene064966	MS.gene010290	PPI
MS.gene064966	MS.gene37955	PPI
MS.gene064966	MS.gene92517	PPI
MS.gene064966	MS.gene34197	PPI
MS.gene064966	MS.gene36069	PPI
MS.gene064966	MS.gene045875	PPI
MS.gene064966	MS.gene84790	PPI
MS.gene064966	MS.gene71675	PPI
MS.gene064966	MS.gene055683	PPI
MS.gene064966	MS.gene044729	PPI
MS.gene010656	MS.gene064966	PPI
MS.gene064966	MS.gene05319	PPI
MS.gene000629	MS.gene064966	PPI
MS.gene064966	MS.gene60390	PPI
MS.gene064966	MS.gene039692	PPI
MS.gene064966	MS.gene032311	PPI
MS.gene05319	MS.gene064966	PPI
MS.gene064966	MS.gene55682	PPI
MS.gene064966	MS.gene002590	PPI
MS.gene064966	MS.gene41979	PPI
MS.gene56991	MS.gene064966	PPI
MS.gene064966	MS.gene008927	PPI
MS.gene055223	MS.gene064966	PPI
MS.gene064966	MS.gene98616	PPI
MS.gene064966	MS.gene23521	PPI
MS.gene28545	MS.gene064966	PPI
MS.gene064966	MS.gene77990	PPI
MS.gene064966	MS.gene65546	PPI
MS.gene064966	MS.gene062511	PPI
MS.gene064966	MS.gene059269	PPI
MS.gene064966	MS.gene057934	PPI
MS.gene061850	MS.gene064966	PPI
MS.gene064966	MS.gene045614	PPI
MS.gene064966	MS.gene024841	PPI
MS.gene064966	MS.gene76420	PPI
MS.gene064966	MS.gene40828	PPI
MS.gene064966	MS.gene87529	PPI
MS.gene064966	MS.gene51799	PPI
MS.gene064966	MS.gene98471	PPI
MS.gene064966	MS.gene062067	PPI
MS.gene98616	MS.gene064966	PPI
MS.gene064966	MS.gene013676	PPI
MS.gene064966	MS.gene29406	PPI
MS.gene69490	MS.gene064966	PPI
MS.gene015248	MS.gene064966	PPI
MS.gene064966	MS.gene09023	PPI
MS.gene064966	MS.gene025987	PPI
MS.gene064966	MS.gene95960	PPI
MS.gene064966	MS.gene043140	PPI
MS.gene72520	MS.gene064966	PPI
MS.gene013193	MS.gene064966	PPI
MS.gene064966	MS.gene041264	PPI
MS.gene064966	MS.gene61452	PPI
MS.gene08106	MS.gene064966	PPI
MS.gene064966	MS.gene03149	PPI
MS.gene064966	MS.gene29321	PPI
MS.gene064966	MS.gene018745	PPI
MS.gene064966	MS.gene025401	PPI
MS.gene064966	MS.gene57044	PPI
MS.gene064966	MS.gene015093	PPI
MS.gene064966	MS.gene24278	PPI
MS.gene064966	MS.gene000379	PPI
MS.gene064966	MS.gene34618	PPI
MS.gene064966	MS.gene59460	PPI
MS.gene064966	MS.gene66650	PPI
MS.gene064966	MS.gene009121	PPI
MS.gene83979	MS.gene064966	PPI
MS.gene062067	MS.gene064966	PPI
MS.gene064966	MS.gene08713	PPI
MS.gene064966	MS.gene70932	PPI
MS.gene064966	MS.gene09517	PPI
MS.gene064966	MS.gene87845	PPI
MS.gene064966	MS.gene024405	PPI
MS.gene57044	MS.gene064966	PPI
MS.gene064966	MS.gene85308	PPI
MS.gene038676	MS.gene064966	PPI
MS.gene064966	MS.gene58419	PPI
MS.gene064966	MS.gene26844	PPI
MS.gene064966	MS.gene64178	PPI
MS.gene042651	MS.gene064966	PPI
MS.gene93396	MS.gene064966	PPI
MS.gene064966	MS.gene074257	PPI
MS.gene064966	MS.gene04749	PPI
MS.gene064966	MS.gene053747	PPI
MS.gene05231	MS.gene064966	PPI
MS.gene064966	MS.gene021555	PPI
MS.gene064966	MS.gene44144	PPI
MS.gene064966	MS.gene05576	PPI
MS.gene064966	MS.gene060718	PPI
MS.gene064966	MS.gene48477	PPI
MS.gene064966	MS.gene055223	PPI
MS.gene064966	MS.gene027169	PPI
MS.gene064966	MS.gene004417	PPI
MS.gene050679	MS.gene064966	PPI
MS.gene064966	MS.gene81712	PPI
MS.gene064966	MS.gene019177	PPI
MS.gene064966	MS.gene031967	PPI
MS.gene064966	MS.gene05436	PPI
MS.gene20246	MS.gene064966	PPI
MS.gene064966	MS.gene038623	PPI
MS.gene064966	MS.gene005120	PPI
MS.gene064966	MS.gene055560	PPI
MS.gene064966	MS.gene009213	PPI
MS.gene064966	MS.gene024297	PPI
MS.gene064966	MS.gene35202	PPI
MS.gene064966	MS.gene054652	PPI
MS.gene064966	MS.gene000634	PPI
MS.gene064966	MS.gene37537	PPI
MS.gene064966	MS.gene069786	PPI
MS.gene064966	MS.gene42010	PPI
MS.gene064966	MS.gene025158	PPI
MS.gene064966	MS.gene049512	PPI
MS.gene064966	MS.gene27329	PPI
MS.gene064966	MS.gene41393	PPI
MS.gene042559	MS.gene064966	PPI
MS.gene22200	MS.gene064966	PPI
MS.gene064966	MS.gene97410	PPI
MS.gene064966	MS.gene010656	PPI
MS.gene064966	MS.gene016632	PPI
MS.gene064966	MS.gene018474	PPI
MS.gene064966	MS.gene40469	PPI
MS.gene064966	MS.gene96163	PPI
MS.gene064966	MS.gene65623	PPI
MS.gene064966	MS.gene012748	PPI
MS.gene064966	MS.gene66651	PPI
MS.gene34812	MS.gene064966	PPI
MS.gene064966	MS.gene02896	PPI
MS.gene064966	MS.gene71588	PPI
MS.gene064966	MS.gene05231	PPI
MS.gene067340	MS.gene064966	PPI
MS.gene064966	MS.gene037049	PPI
MS.gene064966	MS.gene042614	PPI
MS.gene064966	MS.gene026680	PPI
MS.gene064966	MS.gene61499	PPI
MS.gene038623	MS.gene064966	PPI
MS.gene064966	MS.gene051449	PPI
MS.gene05357	MS.gene064966	PPI
MS.gene064966	MS.gene019165	PPI
MS.gene032311	MS.gene064966	PPI
MS.gene064966	MS.gene81194	PPI
MS.gene064966	MS.gene060773	PPI
MS.gene064966	MS.gene038394	PPI
MS.gene064966	MS.gene09589	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064966.t1	MTR_0010s0370	27.778	144	103	1	35	177	34	177	3.53e-22	90.1
MS.gene064966.t1	MTR_0010s0350	27.778	144	103	1	35	177	34	177	3.53e-22	90.1
MS.gene064966.t1	MTR_0008s0210	37.415	147	50	3	48	187	5	116	1.25e-19	82.8
MS.gene064966.t1	MTR_6g033215	28.324	173	118	3	29	197	123	293	2.44e-19	84.7
MS.gene064966.t1	MTR_0008s0180	40.351	114	33	2	74	187	19	97	4.02e-19	80.9
MS.gene064966.t1	MTR_6g045237	27.326	172	124	1	44	214	93	264	3.26e-15	74.3
MS.gene064966.t1	MTR_7g445700	22.527	182	140	1	44	224	91	272	9.15e-12	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064966.t1	AT5G36228	26.901	171	121	3	58	226	62	230	8.35e-14	70.1
MS.gene064966.t1	AT2G01050	28.481	158	108	3	58	214	103	256	1.46e-13	69.7

Find 67 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGAGGTCTTGTACTTCTAC+TGG	0.253343	1.4:+31094894	None:intergenic
ATTCTCGTCGAAGAGGGATT+TGG	0.295523	1.4:-31094850	MS.gene064966:CDS
AGAAATGGAAAACTGGAAAA+AGG	0.326228	1.4:-31095084	None:intergenic
CGAGGAGCAACCGTCAGATA+TGG	0.390838	1.4:-31094760	MS.gene064966:CDS
AGATGCATACTGGTGAGTTC+TGG	0.391082	1.4:-31094737	MS.gene064966:CDS
ACTCTCTGGTTGGAAAGCTT+TGG	0.392070	1.4:-31094983	MS.gene064966:CDS
TGTCCTCGCAATCCTTGATT+TGG	0.392791	1.4:+31094454	None:intergenic
CAGATGGTGTGGAGGAATGT+GGG	0.405082	1.4:-31095022	MS.gene064966:CDS
TGAAGATTCGTCCGTTGAAA+GGG	0.428891	1.4:+31094952	None:intergenic
TAAGGATGAGGCGGAGTTTG+AGG	0.430616	1.4:-31094424	MS.gene064966:CDS
AACAGGTGATTGTCAATGCT+TGG	0.431931	1.4:-31094929	MS.gene064966:CDS
AAAGAAACTTGGTGATCTCC+TGG	0.434651	1.4:-31094667	MS.gene064966:CDS
TGAGATCTTTGCAAACTCTC+TGG	0.435011	1.4:-31094997	MS.gene064966:CDS
ATGGTCCTTGGAGCTTTGAT+AGG	0.454879	1.4:-31094812	MS.gene064966:CDS
GTCAAAGGAGGAGGAAGAAG+AGG	0.460649	1.4:-31095054	MS.gene064966:CDS
TAGCAAGGAAAGTAACAGAT+TGG	0.462196	1.4:-31094625	MS.gene064966:CDS
TGAGGTCTTGTACTTCTACT+GGG	0.465725	1.4:+31094895	None:intergenic
TTGGAGTTAGTTGTGAAGAA+TGG	0.468403	1.4:-31094831	MS.gene064966:CDS
GTGATTCTAAAGCGCATCTC+AGG	0.470185	1.4:-31094783	MS.gene064966:CDS
GTTTCGATTCTCGTCGAAGA+GGG	0.482013	1.4:-31094856	MS.gene064966:CDS
ATCTTTGCAAACTCTCTGGT+TGG	0.483082	1.4:-31094993	MS.gene064966:CDS
CAACGGACGAATCTTCAAAC+AGG	0.487605	1.4:-31094946	MS.gene064966:CDS
TTGAAGATTCGTCCGTTGAA+AGG	0.497019	1.4:+31094951	None:intergenic
AGCAAGGAAAGTAACAGATT+GGG	0.499126	1.4:-31094624	MS.gene064966:CDS
TCAGATATGGAGATGCATAC+TGG	0.500499	1.4:-31094747	MS.gene064966:CDS
AATGAGATGGCAAAGAAACT+TGG	0.507538	1.4:-31094678	MS.gene064966:CDS
TCAAAGCAAATATAGATTTG+AGG	0.509472	1.4:-31094587	MS.gene064966:CDS
AAGAAACTTGGTGATCTCCT+GGG	0.509483	1.4:-31094666	MS.gene064966:CDS
CGCAAATAAAACAGAACGTA+GGG	0.512135	1.4:+31094490	None:intergenic
AATCAAGGATTGCGAGGACA+TGG	0.526201	1.4:-31094451	MS.gene064966:CDS
TTGCGAGGACATGGATGGTA+AGG	0.530899	1.4:-31094442	MS.gene064966:CDS
GCAGATGGTGTGGAGGAATG+TGG	0.535776	1.4:-31095023	MS.gene064966:CDS
GGTAGCACTGTCAAAGGAGG+AGG	0.539008	1.4:-31095063	MS.gene064966:CDS
ATGACCGTTCCTCGTTTCAG+TGG	0.540350	1.4:+31094561	None:intergenic
GAAAATAGGACACCAAATCA+AGG	0.545580	1.4:-31094466	MS.gene064966:CDS
TGGACCACCGACCCTTTCAA+CGG	0.548337	1.4:-31094963	MS.gene064966:CDS
GGAGGAAGAAGAGGGTTTCG+TGG	0.548578	1.4:-31095045	MS.gene064966:CDS
TAGTTGTGAAGAATGGTCCT+TGG	0.548690	1.4:-31094824	MS.gene064966:CDS
AGATGGTGTGGAGGAATGTG+GGG	0.554719	1.4:-31095021	MS.gene064966:CDS
GAAGAGGGTTTCGTGGCAGA+TGG	0.560665	1.4:-31095038	MS.gene064966:CDS
AATCTATATTTGCTTTGACT+CGG	0.560723	1.4:+31094592	None:intergenic
TCATTTGATCTCAGTTTCAG+TGG	0.565666	1.4:+31094696	None:intergenic
GGGTTTCGTGGCAGATGGTG+TGG	0.571477	1.4:-31095033	MS.gene064966:CDS
GGACATGGATGGTAAGGATG+AGG	0.575179	1.4:-31094436	MS.gene064966:CDS
GGAACGGTCATCAAGTATCA+AGG	0.577998	1.4:-31094549	MS.gene064966:CDS
CCTGGGGAAGTTCGTAGAGG+TGG	0.579514	1.4:-31094649	MS.gene064966:CDS
GAAACTGAGATCAAATGAGA+TGG	0.589489	1.4:-31094691	MS.gene064966:CDS
CCACCTCTACGAACTTCCCC+AGG	0.594988	1.4:+31094649	None:intergenic
GTTCGTAGAGGTGGATAGCA+AGG	0.597150	1.4:-31094640	MS.gene064966:CDS
TGTTTCGATTCTCGTCGAAG+AGG	0.610144	1.4:-31094857	MS.gene064966:CDS
TCAAAGGAGGAGGAAGAAGA+GGG	0.612941	1.4:-31095053	MS.gene064966:CDS
TCGTCCGTTGAAAGGGTCGG+TGG	0.628646	1.4:+31094959	None:intergenic
AAGGATTGCGAGGACATGGA+TGG	0.630731	1.4:-31094447	MS.gene064966:CDS
AGAAACTTGGTGATCTCCTG+GGG	0.632093	1.4:-31094665	MS.gene064966:CDS
GATTCGTCCGTTGAAAGGGT+CGG	0.633803	1.4:+31094956	None:intergenic
TCTAAAGCGCATCTCAGGCG+AGG	0.638581	1.4:-31094778	MS.gene064966:CDS
GTATGCATCTCCATATCTGA+CGG	0.639983	1.4:+31094750	None:intergenic
CATGGATGGTAAGGATGAGG+CGG	0.644744	1.4:-31094433	MS.gene064966:CDS
AGGTGATTGTCAATGCTTGG+AGG	0.647999	1.4:-31094926	MS.gene064966:CDS
ACACCAAATCAAGGATTGCG+AGG	0.652058	1.4:-31094457	MS.gene064966:CDS
CCGCAAATAAAACAGAACGT+AGG	0.652598	1.4:+31094489	None:intergenic
AAAGGTAGCACTGTCAAAGG+AGG	0.658642	1.4:-31095066	MS.gene064966:CDS
TTTCGTGGCAGATGGTGTGG+AGG	0.668130	1.4:-31095030	MS.gene064966:CDS
ATATTCCTATCAAAGCTCCA+AGG	0.674347	1.4:+31094807	None:intergenic
TTGAGGAAGCCACTGAAACG+AGG	0.683544	1.4:-31094570	MS.gene064966:CDS
TCTCCTGGGGAAGTTCGTAG+AGG	0.705159	1.4:-31094652	MS.gene064966:CDS
GAAGCCACTGAAACGAGGAA+CGG	0.705887	1.4:-31094565	MS.gene064966:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATCTATATTTGCTTTGACT+CGG	+	chr1.4:31094907-31094926	None:intergenic	25.0%
!	ACAGAAAAAAATTCTTGTTG+AGG	+	chr1.4:31094622-31094641	None:intergenic	25.0%
!	TCAAAGCAAATATAGATTTG+AGG	-	chr1.4:31094909-31094928	MS.gene064966:CDS	25.0%
	GTTCATACTCGAAAAAGATT+CGG	+	chr1.4:31094982-31095001	None:intergenic	30.0%
!!!	GAATCTTTTTCGAGTATGAA+CGG	-	chr1.4:31094981-31095000	MS.gene064966:CDS	30.0%
!!!	TGTTTTATTTGCGGGAAAAT+AGG	-	chr1.4:31095016-31095035	MS.gene064966:CDS	30.0%
	AATGAGATGGCAAAGAAACT+TGG	-	chr1.4:31094818-31094837	MS.gene064966:CDS	35.0%
	AGCAAGGAAAGTAACAGATT+GGG	-	chr1.4:31094872-31094891	MS.gene064966:CDS	35.0%
	ATATTCCTATCAAAGCTCCA+AGG	+	chr1.4:31094692-31094711	None:intergenic	35.0%
	CGCAAATAAAACAGAACGTA+GGG	+	chr1.4:31095009-31095028	None:intergenic	35.0%
	GAAAATAGGACACCAAATCA+AGG	-	chr1.4:31095030-31095049	MS.gene064966:CDS	35.0%
	GAAACTGAGATCAAATGAGA+TGG	-	chr1.4:31094805-31094824	MS.gene064966:CDS	35.0%
	TAGCAAGGAAAGTAACAGAT+TGG	-	chr1.4:31094871-31094890	MS.gene064966:CDS	35.0%
	TCATTTGATCTCAGTTTCAG+TGG	+	chr1.4:31094803-31094822	None:intergenic	35.0%
	TTGGAGTTAGTTGTGAAGAA+TGG	-	chr1.4:31094665-31094684	MS.gene064966:CDS	35.0%
!!!	CCTACGTTCTGTTTTATTTG+CGG	-	chr1.4:31095007-31095026	MS.gene064966:CDS	35.0%
!!!	CTACGTTCTGTTTTATTTGC+GGG	-	chr1.4:31095008-31095027	MS.gene064966:CDS	35.0%
	AACAGGTGATTGTCAATGCT+TGG	-	chr1.4:31094567-31094586	MS.gene064966:CDS	40.0%
	ATCTTTGCAAACTCTCTGGT+TGG	-	chr1.4:31094503-31094522	MS.gene064966:CDS	40.0%
	CCGCAAATAAAACAGAACGT+AGG	+	chr1.4:31095010-31095029	None:intergenic	40.0%
	GAAAAAGGTAGCACTGTCAA+AGG	-	chr1.4:31094427-31094446	MS.gene064966:CDS	40.0%
	GTATGCATCTCCATATCTGA+CGG	+	chr1.4:31094749-31094768	None:intergenic	40.0%
	TAGTTGTGAAGAATGGTCCT+TGG	-	chr1.4:31094672-31094691	MS.gene064966:CDS	40.0%
	TCAGATATGGAGATGCATAC+TGG	-	chr1.4:31094749-31094768	MS.gene064966:CDS	40.0%
	TGAAGATTCGTCCGTTGAAA+GGG	+	chr1.4:31094547-31094566	None:intergenic	40.0%
	TGAGATCTTTGCAAACTCTC+TGG	-	chr1.4:31094499-31094518	MS.gene064966:CDS	40.0%
	TTGAAGATTCGTCCGTTGAA+AGG	+	chr1.4:31094548-31094567	None:intergenic	40.0%
!	AAAGAAACTTGGTGATCTCC+TGG	-	chr1.4:31094829-31094848	MS.gene064966:CDS	40.0%
!	AAGAAACTTGGTGATCTCCT+GGG	-	chr1.4:31094830-31094849	MS.gene064966:CDS	40.0%
!	TGAGGTCTTGTACTTCTACT+GGG	+	chr1.4:31094604-31094623	None:intergenic	40.0%
!	TTGAGGTCTTGTACTTCTAC+TGG	+	chr1.4:31094605-31094624	None:intergenic	40.0%
	AAAGGTAGCACTGTCAAAGG+AGG	-	chr1.4:31094430-31094449	MS.gene064966:CDS	45.0%
	AATCAAGGATTGCGAGGACA+TGG	-	chr1.4:31095045-31095064	MS.gene064966:CDS	45.0%
	ACACCAAATCAAGGATTGCG+AGG	-	chr1.4:31095039-31095058	MS.gene064966:CDS	45.0%
	ACTCTCTGGTTGGAAAGCTT+TGG	-	chr1.4:31094513-31094532	MS.gene064966:CDS	45.0%
	AGATGCATACTGGTGAGTTC+TGG	-	chr1.4:31094759-31094778	MS.gene064966:CDS	45.0%
	AGGTGATTGTCAATGCTTGG+AGG	-	chr1.4:31094570-31094589	MS.gene064966:CDS	45.0%
	ATTCTCGTCGAAGAGGGATT+TGG	-	chr1.4:31094646-31094665	MS.gene064966:CDS	45.0%
	CAACGGACGAATCTTCAAAC+AGG	-	chr1.4:31094550-31094569	MS.gene064966:CDS	45.0%
	GGAACGGTCATCAAGTATCA+AGG	-	chr1.4:31094947-31094966	MS.gene064966:CDS	45.0%
	GTTTCGATTCTCGTCGAAGA+GGG	-	chr1.4:31094640-31094659	MS.gene064966:CDS	45.0%
	TCAAAGGAGGAGGAAGAAGA+GGG	-	chr1.4:31094443-31094462	MS.gene064966:CDS	45.0%
	TGTCCTCGCAATCCTTGATT+TGG	+	chr1.4:31095045-31095064	None:intergenic	45.0%
	TGTTTCGATTCTCGTCGAAG+AGG	-	chr1.4:31094639-31094658	MS.gene064966:CDS	45.0%
!	AGAAACTTGGTGATCTCCTG+GGG	-	chr1.4:31094831-31094850	MS.gene064966:CDS	45.0%
!	ATGGTCCTTGGAGCTTTGAT+AGG	-	chr1.4:31094684-31094703	MS.gene064966:CDS	45.0%
!!	GTGATTCTAAAGCGCATCTC+AGG	-	chr1.4:31094713-31094732	MS.gene064966:CDS	45.0%
	AAGGATTGCGAGGACATGGA+TGG	-	chr1.4:31095049-31095068	MS.gene064966:CDS	50.0%
	AGATGGTGTGGAGGAATGTG+GGG	-	chr1.4:31094475-31094494	MS.gene064966:CDS	50.0%
	ATGACCGTTCCTCGTTTCAG+TGG	+	chr1.4:31094938-31094957	None:intergenic	50.0%
	CAGATGGTGTGGAGGAATGT+GGG	-	chr1.4:31094474-31094493	MS.gene064966:CDS	50.0%
	CATGGATGGTAAGGATGAGG+CGG	-	chr1.4:31095063-31095082	MS.gene064966:CDS	50.0%
	GAAGCCACTGAAACGAGGAA+CGG	-	chr1.4:31094931-31094950	MS.gene064966:CDS	50.0%
	GATTCGTCCGTTGAAAGGGT+CGG	+	chr1.4:31094543-31094562	None:intergenic	50.0%
	GGACATGGATGGTAAGGATG+AGG	-	chr1.4:31095060-31095079	MS.gene064966:CDS	50.0%
	GTCAAAGGAGGAGGAAGAAG+AGG	-	chr1.4:31094442-31094461	MS.gene064966:CDS	50.0%
	GTTCGTAGAGGTGGATAGCA+AGG	-	chr1.4:31094856-31094875	MS.gene064966:CDS	50.0%
	TAAGGATGAGGCGGAGTTTG+AGG	-	chr1.4:31095072-31095091	MS.gene064966:CDS	50.0%
	TTGAGGAAGCCACTGAAACG+AGG	-	chr1.4:31094926-31094945	MS.gene064966:CDS	50.0%
	TTGCGAGGACATGGATGGTA+AGG	-	chr1.4:31095054-31095073	MS.gene064966:CDS	50.0%
	CGAGGAGCAACCGTCAGATA+TGG	-	chr1.4:31094736-31094755	MS.gene064966:CDS	55.0%
	GAAGAGGGTTTCGTGGCAGA+TGG	-	chr1.4:31094458-31094477	MS.gene064966:CDS	55.0%
	GCAGATGGTGTGGAGGAATG+TGG	-	chr1.4:31094473-31094492	MS.gene064966:CDS	55.0%
	GGAGGAAGAAGAGGGTTTCG+TGG	-	chr1.4:31094451-31094470	MS.gene064966:CDS	55.0%
	GGTAGCACTGTCAAAGGAGG+AGG	-	chr1.4:31094433-31094452	MS.gene064966:CDS	55.0%
	TCTCCTGGGGAAGTTCGTAG+AGG	-	chr1.4:31094844-31094863	MS.gene064966:CDS	55.0%
	TGGACCACCGACCCTTTCAA+CGG	-	chr1.4:31094533-31094552	MS.gene064966:CDS	55.0%
	TTTCGTGGCAGATGGTGTGG+AGG	-	chr1.4:31094466-31094485	MS.gene064966:CDS	55.0%
!	TCTAAAGCGCATCTCAGGCG+AGG	-	chr1.4:31094718-31094737	MS.gene064966:CDS	55.0%
	CCACCTCTACGAACTTCCCC+AGG	+	chr1.4:31094850-31094869	None:intergenic	60.0%
	CCTGGGGAAGTTCGTAGAGG+TGG	-	chr1.4:31094847-31094866	MS.gene064966:CDS	60.0%
	GGGTTTCGTGGCAGATGGTG+TGG	-	chr1.4:31094463-31094482	MS.gene064966:CDS	60.0%
	TCGTCCGTTGAAAGGGTCGG+TGG	+	chr1.4:31094540-31094559	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	31094416	31095102	31094416	ID=MS.gene064966
chr1.4	mRNA	31094416	31095102	31094416	ID=MS.gene064966.t1;Parent=MS.gene064966
chr1.4	exon	31094416	31095102	31094416	ID=MS.gene064966.t1.exon1;Parent=MS.gene064966.t1
chr1.4	CDS	31094416	31095102	31094416	ID=cds.MS.gene064966.t1;Parent=MS.gene064966.t1

Gene Sequence

>MS.gene064966

ATGGAAAACTGGAAAAAGGTAGCACTGTCAAAGGAGGAGGAAGAAGAGGGTTTCGTGGCAGATGGTGTGGAGGAATGTGGGGATGAGATCTTTGCAAACTCTCTGGTTGGAAAGCTTTGGACCACCGACCCTTTCAACGGACGAATCTTCAAACAGGTGATTGTCAATGCTTGGAGGCTGAAGAACCCAGTAGAAGTACAAGACCTCAACAAGAATTTTTTTCTGTTTCGATTCTCGTCGAAGAGGGATTTGGAGTTAGTTGTGAAGAATGGTCCTTGGAGCTTTGATAGGAATATTGTGATTCTAAAGCGCATCTCAGGCGAGGAGCAACCGTCAGATATGGAGATGCATACTGGTGAGTTCTGGACTAGAATCTACGATTTACCACTGAAACTGAGATCAAATGAGATGGCAAAGAAACTTGGTGATCTCCTGGGGAAGTTCGTAGAGGTGGATAGCAAGGAAAGTAACAGATTGGGTTGCTTTCTCCGAGTCAAAGCAAATATAGATTTGAGGAAGCCACTGAAACGAGGAACGGTCATCAAGTATCAAGGTAAAAATCTCCGAATCTTTTTCGAGTATGAACGGTTCCCTACGTTCTGTTTTATTTGCGGGAAAATAGGACACCAAATCAAGGATTGCGAGGACATGGATGGTAAGGATGAGGCGGAGTTTGAGGAATTATAA

Protein sequence

>MS.gene064966.t1

MENWKKVALSKEEEEEGFVADGVEECGDEIFANSLVGKLWTTDPFNGRIFKQVIVNAWRLKNPVEVQDLNKNFFLFRFSSKRDLELVVKNGPWSFDRNIVILKRISGEEQPSDMEMHTGEFWTRIYDLPLKLRSNEMAKKLGDLLGKFVEVDSKESNRLGCFLRVKANIDLRKPLKRGTVIKYQGKNLRIFFEYERFPTFCFICGKIGHQIKDCEDMDGKDEAEFEEL