AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0138370


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0138370 MtrunA17_Chr2g0291491 97.619 126 3 0 2 127 450 575 2.34e-82 251
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0138370 AT3G54630.1 44.037 109 61 0 1 109 454 562 2.99e-27 105

Find 29 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAATACACGAGCAAAATA+TGG 0.313368 1_3:-71236212 None:intergenic
CACTCTTTGTTGGAGTTAGA+AGG 0.338244 1_3:+71235020 Msa0138370:five_prime_UTR
TGATCATGATGTGGGCATTA+TGG 0.365375 1_3:+71235224 Msa0138370:CDS
GTTTATGTTACACTCTTTGT+TGG 0.374247 1_3:+71235010 Msa0138370:five_prime_UTR
GCATCTTTACATCAAATTGA+TGG 0.380197 1_3:-71236241 None:intergenic
GTCCGACATCTTCTTAGCTT+CGG 0.401875 1_3:-71235187 None:intergenic
GGCATCTGAATTGAAGTTGC+AGG 0.406818 1_3:+71235794 Msa0138370:intron
AATGTGGTTAATTGAAAGTA+GGG 0.409020 1_3:+71236119 Msa0138370:three_prime_UTR
TCACTAGCTGGAAACTCAAA+TGG 0.437352 1_3:+71235119 Msa0138370:exon
GAATGTGGTTAATTGAAAGT+AGG 0.443207 1_3:+71236118 Msa0138370:three_prime_UTR
AGAGGCAGCTGAGGTTCTAA+AGG 0.469279 1_3:+71235251 Msa0138370:CDS
AACAAGCAGTAGAAAAGAAA+AGG 0.477440 1_3:-71235089 None:intergenic
TTGTTGATTTATTCACTAGC+TGG 0.483818 1_3:+71235107 Msa0138370:five_prime_UTR
AAAATATAGTTATATATTGG+AGG 0.526340 1_3:+71235992 Msa0138370:CDS
CTCAAAGTATAAAGAACATG+TGG 0.527765 1_3:+71235887 Msa0138370:CDS
CCACATCATGATCAGCTAGC+TGG 0.533202 1_3:-71235215 None:intergenic
ACTCTTTGTTGGAGTTAGAA+GGG 0.534990 1_3:+71235021 Msa0138370:five_prime_UTR
GAGCAAAATATGGAGCTGAA+TGG 0.581103 1_3:-71236202 None:intergenic
CCAGCTAGCTGATCATGATG+TGG 0.595217 1_3:+71235215 Msa0138370:CDS
GTCAAACAACAGTCTTTACA+AGG 0.603205 1_3:-71235067 None:intergenic
GAAGAAATTCAAATGCATGC+CGG 0.605310 1_3:+71235837 Msa0138370:CDS
AAAGTTTCAGAGATTAGAAG+CGG 0.616241 1_3:+71235915 Msa0138370:CDS
AAGTTTCAGAGATTAGAAGC+GGG 0.618896 1_3:+71235916 Msa0138370:CDS
CATGATAAAGAAAGAAACAC+AGG 0.630583 1_3:+71235143 Msa0138370:CDS
TCAAAGTATAAAGAACATGT+GGG 0.633300 1_3:+71235888 Msa0138370:CDS
TATGGAAAGAGAGGCAGCTG+AGG 0.635705 1_3:+71235242 Msa0138370:CDS
CAGCTAGCTGATCATGATGT+GGG 0.635721 1_3:+71235216 Msa0138370:CDS
TGTGGGCATTATGGAAAGAG+AGG 0.640887 1_3:+71235233 Msa0138370:CDS
TGCCGAAGCTAAGAAGATGT+CGG 0.664082 1_3:+71235185 Msa0138370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATATAACTATATTTTTCT+GGG - chr1_3:71235989-71236008 None:intergenic 10.0%
!!! AATATATAACTATATTTTTC+TGG - chr1_3:71235990-71236009 None:intergenic 10.0%
!! AGAAAAATATAGTTATATAT+TGG + chr1_3:71235989-71236008 Msa0138370:CDS 10.0%
!!! ATATATAACTATATTTTTCT+GGG - chr1_3:71235989-71236008 None:intergenic 10.0%
!!! AATATATAACTATATTTTTC+TGG - chr1_3:71235990-71236009 None:intergenic 10.0%
!! AGAAAAATATAGTTATATAT+TGG + chr1_3:71235989-71236008 Msa0138370:CDS 10.0%
!! TAAAGATAAAAATAAGAATC+CGG - chr1_3:71235283-71235302 None:intergenic 15.0%
!!! TTGTGTTTCAATTATTTTTT+TGG + chr1_3:71235522-71235541 Msa0138370:intron 15.0%
!! AAAATATAGTTATATATTGG+AGG + chr1_3:71235992-71236011 Msa0138370:CDS 15.0%
!! TAAAGATAAAAATAAGAATC+CGG - chr1_3:71235283-71235302 None:intergenic 15.0%
!!! TTGTGTTTCAATTATTTTTT+TGG + chr1_3:71235522-71235541 Msa0138370:intron 15.0%
!! AAAATATAGTTATATATTGG+AGG + chr1_3:71235992-71236011 Msa0138370:CDS 15.0%
!! AAAAAATCCAAAAACTAAAC+TGG - chr1_3:71235473-71235492 None:intergenic 20.0%
!! TAGTAAATGATAATCAAACT+GGG + chr1_3:71235666-71235685 Msa0138370:intron 20.0%
!!! AACTTTTTATATTGTTGTCA+AGG + chr1_3:71235773-71235792 Msa0138370:intron 20.0%
!! AATCAACTTAAAAAGTTTAC+CGG - chr1_3:71235859-71235878 None:intergenic 20.0%
!!! ATTCAATTCTCTGAATTTTA+CGG - chr1_3:71236022-71236041 None:intergenic 20.0%
!!! CTTTTAAATATGAATGAATG+TGG + chr1_3:71236103-71236122 Msa0138370:three_prime_UTR 20.0%
!! AAAAAATCCAAAAACTAAAC+TGG - chr1_3:71235473-71235492 None:intergenic 20.0%
!! TAGTAAATGATAATCAAACT+GGG + chr1_3:71235666-71235685 Msa0138370:intron 20.0%
!!! AACTTTTTATATTGTTGTCA+AGG + chr1_3:71235773-71235792 Msa0138370:intron 20.0%
!! AATCAACTTAAAAAGTTTAC+CGG - chr1_3:71235859-71235878 None:intergenic 20.0%
!!! ATTCAATTCTCTGAATTTTA+CGG - chr1_3:71236022-71236041 None:intergenic 20.0%
!!! CTTTTAAATATGAATGAATG+TGG + chr1_3:71236103-71236122 Msa0138370:three_prime_UTR 20.0%
! AGTTGTATTTGAACCAAATT+GGG + chr1_3:71235314-71235333 Msa0138370:intron 25.0%
! TTATTGTGTTTCAATGAACT+TGG + chr1_3:71235401-71235420 Msa0138370:intron 25.0%
! AATTGAAACACAAGATCAAT+GGG - chr1_3:71235515-71235534 None:intergenic 25.0%
! TAATTGAAACACAAGATCAA+TGG - chr1_3:71235516-71235535 None:intergenic 25.0%
! CAGTTTGATTATCATTTACT+AGG - chr1_3:71235667-71235686 None:intergenic 25.0%
! CTAGTAAATGATAATCAAAC+TGG + chr1_3:71235665-71235684 Msa0138370:intron 25.0%
! ACAATGTTGTCACATAAATT+GGG - chr1_3:71235745-71235764 None:intergenic 25.0%
! TACAATGTTGTCACATAAAT+TGG - chr1_3:71235746-71235765 None:intergenic 25.0%
! TCAAAGTATAAAGAACATGT+GGG + chr1_3:71235888-71235907 Msa0138370:CDS 25.0%
! AATGTGGTTAATTGAAAGTA+GGG + chr1_3:71236119-71236138 Msa0138370:three_prime_UTR 25.0%
!!! AAAGTAGGGTTTTTAGTATT+GGG + chr1_3:71236133-71236152 Msa0138370:three_prime_UTR 25.0%
! AGTTGTATTTGAACCAAATT+GGG + chr1_3:71235314-71235333 Msa0138370:intron 25.0%
! TTATTGTGTTTCAATGAACT+TGG + chr1_3:71235401-71235420 Msa0138370:intron 25.0%
! AATTGAAACACAAGATCAAT+GGG - chr1_3:71235515-71235534 None:intergenic 25.0%
! TAATTGAAACACAAGATCAA+TGG - chr1_3:71235516-71235535 None:intergenic 25.0%
! CAGTTTGATTATCATTTACT+AGG - chr1_3:71235667-71235686 None:intergenic 25.0%
! CTAGTAAATGATAATCAAAC+TGG + chr1_3:71235665-71235684 Msa0138370:intron 25.0%
! ACAATGTTGTCACATAAATT+GGG - chr1_3:71235745-71235764 None:intergenic 25.0%
! TACAATGTTGTCACATAAAT+TGG - chr1_3:71235746-71235765 None:intergenic 25.0%
! TCAAAGTATAAAGAACATGT+GGG + chr1_3:71235888-71235907 Msa0138370:CDS 25.0%
! AATGTGGTTAATTGAAAGTA+GGG + chr1_3:71236119-71236138 Msa0138370:three_prime_UTR 25.0%
!!! AAAGTAGGGTTTTTAGTATT+GGG + chr1_3:71236133-71236152 Msa0138370:three_prime_UTR 25.0%
GTTTATGTTACACTCTTTGT+TGG + chr1_3:71235010-71235029 Msa0138370:five_prime_UTR 30.0%
AACAAGCAGTAGAAAAGAAA+AGG - chr1_3:71235092-71235111 None:intergenic 30.0%
!! TTGTTGATTTATTCACTAGC+TGG + chr1_3:71235107-71235126 Msa0138370:five_prime_UTR 30.0%
CATGATAAAGAAAGAAACAC+AGG + chr1_3:71235143-71235162 Msa0138370:CDS 30.0%
CAGTTGTATTTGAACCAAAT+TGG + chr1_3:71235313-71235332 Msa0138370:intron 30.0%
GTTGTATTTGAACCAAATTG+GGG + chr1_3:71235315-71235334 Msa0138370:intron 30.0%
TTGTATTTGAACCAAATTGG+GGG + chr1_3:71235316-71235335 Msa0138370:intron 30.0%
AATATGCATTCCACATTGAT+TGG + chr1_3:71235436-71235455 Msa0138370:intron 30.0%
AAGCTTGCAATAAAAAGGAA+TGG - chr1_3:71235637-71235656 None:intergenic 30.0%
! TTCCTTTTTATTGCAAGCTT+TGG + chr1_3:71235637-71235656 Msa0138370:intron 30.0%
! ATTGGGAAAGATTTAAAACG+AGG - chr1_3:71235728-71235747 None:intergenic 30.0%
CTCAAAGTATAAAGAACATG+TGG + chr1_3:71235887-71235906 Msa0138370:CDS 30.0%
AAAGTTTCAGAGATTAGAAG+CGG + chr1_3:71235915-71235934 Msa0138370:CDS 30.0%
GAATGTGGTTAATTGAAAGT+AGG + chr1_3:71236118-71236137 Msa0138370:three_prime_UTR 30.0%
!!! GAAAGTAGGGTTTTTAGTAT+TGG + chr1_3:71236132-71236151 Msa0138370:three_prime_UTR 30.0%
ATGAATACACGAGCAAAATA+TGG - chr1_3:71236215-71236234 None:intergenic 30.0%
CCATCAATTTGATGTAAAGA+TGG + chr1_3:71236241-71236260 Msa0138370:three_prime_UTR 30.0%
GTTTATGTTACACTCTTTGT+TGG + chr1_3:71235010-71235029 Msa0138370:five_prime_UTR 30.0%
AACAAGCAGTAGAAAAGAAA+AGG - chr1_3:71235092-71235111 None:intergenic 30.0%
!! TTGTTGATTTATTCACTAGC+TGG + chr1_3:71235107-71235126 Msa0138370:five_prime_UTR 30.0%
CATGATAAAGAAAGAAACAC+AGG + chr1_3:71235143-71235162 Msa0138370:CDS 30.0%
CAGTTGTATTTGAACCAAAT+TGG + chr1_3:71235313-71235332 Msa0138370:intron 30.0%
GTTGTATTTGAACCAAATTG+GGG + chr1_3:71235315-71235334 Msa0138370:intron 30.0%
TTGTATTTGAACCAAATTGG+GGG + chr1_3:71235316-71235335 Msa0138370:intron 30.0%
AATATGCATTCCACATTGAT+TGG + chr1_3:71235436-71235455 Msa0138370:intron 30.0%
AAGCTTGCAATAAAAAGGAA+TGG - chr1_3:71235637-71235656 None:intergenic 30.0%
! TTCCTTTTTATTGCAAGCTT+TGG + chr1_3:71235637-71235656 Msa0138370:intron 30.0%
! ATTGGGAAAGATTTAAAACG+AGG - chr1_3:71235728-71235747 None:intergenic 30.0%
CTCAAAGTATAAAGAACATG+TGG + chr1_3:71235887-71235906 Msa0138370:CDS 30.0%
AAAGTTTCAGAGATTAGAAG+CGG + chr1_3:71235915-71235934 Msa0138370:CDS 30.0%
GAATGTGGTTAATTGAAAGT+AGG + chr1_3:71236118-71236137 Msa0138370:three_prime_UTR 30.0%
!!! GAAAGTAGGGTTTTTAGTAT+TGG + chr1_3:71236132-71236151 Msa0138370:three_prime_UTR 30.0%
ATGAATACACGAGCAAAATA+TGG - chr1_3:71236215-71236234 None:intergenic 30.0%
ACTCTTTGTTGGAGTTAGAA+GGG + chr1_3:71235021-71235040 Msa0138370:five_prime_UTR 35.0%
GTCAAACAACAGTCTTTACA+AGG - chr1_3:71235070-71235089 None:intergenic 35.0%
!! GAGGTTCTAAAGGTAAAATC+CGG + chr1_3:71235261-71235280 Msa0138370:intron 35.0%
TTGTGTTTCAATGAACTTGG+AGG + chr1_3:71235404-71235423 Msa0138370:intron 35.0%
TGTGTTTCAATGAACTTGGA+GGG + chr1_3:71235405-71235424 Msa0138370:intron 35.0%
AAACTAAACTGGCACGAAAT+TGG - chr1_3:71235462-71235481 None:intergenic 35.0%
!!! TTTCGTGCCAGTTTAGTTTT+TGG + chr1_3:71235463-71235482 Msa0138370:intron 35.0%
TACGAAAAACATGCTAGATG+TGG - chr1_3:71235603-71235622 None:intergenic 35.0%
AGCCAAAGCTTGCAATAAAA+AGG - chr1_3:71235642-71235661 None:intergenic 35.0%
GAAGAAATTCAAATGCATGC+CGG + chr1_3:71235837-71235856 Msa0138370:CDS 35.0%
AAGTTTCAGAGATTAGAAGC+GGG + chr1_3:71235916-71235935 Msa0138370:CDS 35.0%
ACTCTTTGTTGGAGTTAGAA+GGG + chr1_3:71235021-71235040 Msa0138370:five_prime_UTR 35.0%
GTCAAACAACAGTCTTTACA+AGG - chr1_3:71235070-71235089 None:intergenic 35.0%
!! GAGGTTCTAAAGGTAAAATC+CGG + chr1_3:71235261-71235280 Msa0138370:intron 35.0%
TTGTGTTTCAATGAACTTGG+AGG + chr1_3:71235404-71235423 Msa0138370:intron 35.0%
TGTGTTTCAATGAACTTGGA+GGG + chr1_3:71235405-71235424 Msa0138370:intron 35.0%
AAACTAAACTGGCACGAAAT+TGG - chr1_3:71235462-71235481 None:intergenic 35.0%
!!! TTTCGTGCCAGTTTAGTTTT+TGG + chr1_3:71235463-71235482 Msa0138370:intron 35.0%
TACGAAAAACATGCTAGATG+TGG - chr1_3:71235603-71235622 None:intergenic 35.0%
AGCCAAAGCTTGCAATAAAA+AGG - chr1_3:71235642-71235661 None:intergenic 35.0%
GAAGAAATTCAAATGCATGC+CGG + chr1_3:71235837-71235856 Msa0138370:CDS 35.0%
AAGTTTCAGAGATTAGAAGC+GGG + chr1_3:71235916-71235935 Msa0138370:CDS 35.0%
CACTCTTTGTTGGAGTTAGA+AGG + chr1_3:71235020-71235039 Msa0138370:five_prime_UTR 40.0%
TCACTAGCTGGAAACTCAAA+TGG + chr1_3:71235119-71235138 Msa0138370:exon 40.0%
TGATCATGATGTGGGCATTA+TGG + chr1_3:71235224-71235243 Msa0138370:CDS 40.0%
TTGAACCAAATTGGGGGAAA+AGG + chr1_3:71235322-71235341 Msa0138370:intron 40.0%
ACGAAATTGGCCAATCAATG+TGG - chr1_3:71235449-71235468 None:intergenic 40.0%
GAGCAAAATATGGAGCTGAA+TGG - chr1_3:71236205-71236224 None:intergenic 40.0%
CACTCTTTGTTGGAGTTAGA+AGG + chr1_3:71235020-71235039 Msa0138370:five_prime_UTR 40.0%
TCACTAGCTGGAAACTCAAA+TGG + chr1_3:71235119-71235138 Msa0138370:exon 40.0%
TGATCATGATGTGGGCATTA+TGG + chr1_3:71235224-71235243 Msa0138370:CDS 40.0%
TTGAACCAAATTGGGGGAAA+AGG + chr1_3:71235322-71235341 Msa0138370:intron 40.0%
ACGAAATTGGCCAATCAATG+TGG - chr1_3:71235449-71235468 None:intergenic 40.0%
GAGCAAAATATGGAGCTGAA+TGG - chr1_3:71236205-71236224 None:intergenic 40.0%
TGCCGAAGCTAAGAAGATGT+CGG + chr1_3:71235185-71235204 Msa0138370:CDS 45.0%
GTCCGACATCTTCTTAGCTT+CGG - chr1_3:71235190-71235209 None:intergenic 45.0%
CAGCTAGCTGATCATGATGT+GGG + chr1_3:71235216-71235235 Msa0138370:CDS 45.0%
TGTGGGCATTATGGAAAGAG+AGG + chr1_3:71235233-71235252 Msa0138370:CDS 45.0%
TGGGTAGCAACTAGCATCTT+AGG - chr1_3:71235496-71235515 None:intergenic 45.0%
GGCATCTGAATTGAAGTTGC+AGG + chr1_3:71235794-71235813 Msa0138370:intron 45.0%
TGCCGAAGCTAAGAAGATGT+CGG + chr1_3:71235185-71235204 Msa0138370:CDS 45.0%
GTCCGACATCTTCTTAGCTT+CGG - chr1_3:71235190-71235209 None:intergenic 45.0%
CAGCTAGCTGATCATGATGT+GGG + chr1_3:71235216-71235235 Msa0138370:CDS 45.0%
TGTGGGCATTATGGAAAGAG+AGG + chr1_3:71235233-71235252 Msa0138370:CDS 45.0%
TGGGTAGCAACTAGCATCTT+AGG - chr1_3:71235496-71235515 None:intergenic 45.0%
GGCATCTGAATTGAAGTTGC+AGG + chr1_3:71235794-71235813 Msa0138370:intron 45.0%
CCACATCATGATCAGCTAGC+TGG - chr1_3:71235218-71235237 None:intergenic 50.0%
CCAGCTAGCTGATCATGATG+TGG + chr1_3:71235215-71235234 Msa0138370:CDS 50.0%
TATGGAAAGAGAGGCAGCTG+AGG + chr1_3:71235242-71235261 Msa0138370:CDS 50.0%
! AGAGGCAGCTGAGGTTCTAA+AGG + chr1_3:71235251-71235270 Msa0138370:CDS 50.0%
! CAACGCCTTTTCCCCCAATT+TGG - chr1_3:71235330-71235349 None:intergenic 50.0%
! CAAATTGGGGGAAAAGGCGT+TGG + chr1_3:71235328-71235347 Msa0138370:intron 50.0%
CCACATCATGATCAGCTAGC+TGG - chr1_3:71235218-71235237 None:intergenic 50.0%
CCAGCTAGCTGATCATGATG+TGG + chr1_3:71235215-71235234 Msa0138370:CDS 50.0%
TATGGAAAGAGAGGCAGCTG+AGG + chr1_3:71235242-71235261 Msa0138370:CDS 50.0%
! AGAGGCAGCTGAGGTTCTAA+AGG + chr1_3:71235251-71235270 Msa0138370:CDS 50.0%
! CAACGCCTTTTCCCCCAATT+TGG - chr1_3:71235330-71235349 None:intergenic 50.0%
! CAAATTGGGGGAAAAGGCGT+TGG + chr1_3:71235328-71235347 Msa0138370:intron 50.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 71235010 71236262 71235010 ID=Msa0138370;Name=Msa0138370
chr1_3 mRNA 71235010 71236262 71235010 ID=Msa0138370-mRNA-1;Parent=Msa0138370;Name=Msa0138370-mRNA-1;_AED=0.00;_eAED=0.00;_QI=128|1|1|1|1|1|2|219|127
chr1_3 exon 71235010 71235272 71235010 ID=Msa0138370-mRNA-1:exon:24759;Parent=Msa0138370-mRNA-1
chr1_3 exon 71235795 71236262 71235795 ID=Msa0138370-mRNA-1:exon:24760;Parent=Msa0138370-mRNA-1
chr1_3 five_prime_UTR 71235010 71235137 71235010 ID=Msa0138370-mRNA-1:five_prime_utr;Parent=Msa0138370-mRNA-1
chr1_3 CDS 71235138 71235272 71235138 ID=Msa0138370-mRNA-1:cds;Parent=Msa0138370-mRNA-1
chr1_3 CDS 71235795 71236043 71235795 ID=Msa0138370-mRNA-1:cds;Parent=Msa0138370-mRNA-1
chr1_3 three_prime_UTR 71236044 71236262 71236044 ID=Msa0138370-mRNA-1:three_prime_utr;Parent=Msa0138370-mRNA-1
Gene Sequence

>Msa0138370

ATGGACATGATAAAGAAAGAAACACAGGACTATAGAAATAGATGTTCTGCCGAAGCTAAGAAGATGTCGGACGATATCCAGCTAGCTGATCATGATGTGGGCATTATGGAAAGAGAGGCAGCTGAGGTTCTAAAGGCATCTGAATTGAAGTTGCAGGAAACGATTAGACAAACTGATGAAGAAATTCAAATGCATGCCGGTAAACTTTTTAAGTTGATTGATGCAGTCTCAAAGTATAAAGAACATGTGGGATCTAAAGTTTCAGAGATTAGAAGCGGGCTATCAGAAACTGTAACTGAAGTCTCAAATATATACAGAGATTCATTCCCAGAAAAATATAGTTATATATTGGAGGCAAGCCGTAAAATTCAGAGAATTGAATAA

Protein sequence

>Msa0138370

MDMIKKETQDYRNRCSAEAKKMSDDIQLADHDVGIMEREAAEVLKASELKLQETIRQTDEEIQMHAGKLFKLIDAVSKYKEHVGSKVSEIRSGLSETVTEVSNIYRDSFPEKYSYILEASRKIQRIE*