AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1212090


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1212090 MtrunA17_Chr4g0060821 82.222 90 15 1 1 89 1 90 5.67e-45 139
Msa1212090 MtrunA17_Chr3g0092121 55.435 92 36 2 1 89 1 90 9.53e-29 99.0
Msa1212090 MtrunA17_Chr6g0460751 38.889 90 49 3 1 89 1 85 2.11e-12 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTTATTCGTGATTCTTCT+TGG 0.169086 8_1:-71939273 Msa1212090:CDS
ATTAAGCACAAAACTGAAAA+AGG 0.192325 8_1:-71938364 Msa1212090:three_prime_UTR
TTGTTTATGTATGGGATCTT+AGG 0.200401 8_1:-71938534 Msa1212090:three_prime_UTR
TCCATCCTCTCATTAATTCT+TGG 0.236814 8_1:+71938751 None:intergenic
CTCTCATTAATTCTTGGCTT+TGG 0.277804 8_1:+71938757 None:intergenic
TAGAAGAATTTACTTGTAAC+TGG 0.288724 8_1:+71939219 None:intergenic
AATTAATGAGAGGATGGATT+TGG 0.333806 8_1:-71938746 Msa1212090:CDS
AAATCCCCATTGTTTATGTA+TGG 0.334660 8_1:-71938543 Msa1212090:three_prime_UTR
CTCAATGATTATGCACCATC+TGG 0.362640 8_1:-71938721 Msa1212090:CDS
AGAGGAACTAACATTTAATC+AGG 0.392881 8_1:+71938654 None:intergenic
AAAAGGGTCCATATTCATAT+TGG 0.393272 8_1:-71938347 Msa1212090:three_prime_UTR
CAACTTGGACATCTTGCATA+AGG 0.415467 8_1:+71938948 None:intergenic
AATCAGGTTACACACCATTA+CGG 0.442297 8_1:+71938670 None:intergenic
ATAAATTGAGAACTAGCATA+TGG 0.454361 8_1:-71939366 Msa1212090:five_prime_UTR
AGATCCCATACATAAACAAT+GGG 0.456560 8_1:+71938538 None:intergenic
AATATATAGGTGGACGGTAG+AGG 0.459504 8_1:+71938314 None:intergenic
ACACACCATTACGGTGCTCT+TGG 0.466740 8_1:+71938679 None:intergenic
TATGCACCATCTGGGGCAAA+TGG 0.473723 8_1:-71938712 Msa1212090:CDS
GAGAAGGGTCATTGCAGTGC+AGG 0.473866 8_1:-71939315 Msa1212090:five_prime_UTR
CATTGCAGTGCAGGAATGGC+AGG 0.490014 8_1:-71939306 Msa1212090:exon
GGGTCATTGCAGTGCAGGAA+TGG 0.499077 8_1:-71939310 Msa1212090:exon
ATCATTGAAATTCTAGTACA+TGG 0.502495 8_1:+71938476 None:intergenic
ATAAAATAAGACAAAATCAA+TGG 0.507774 8_1:+71938422 None:intergenic
AATCCCCATTGTTTATGTAT+GGG 0.510722 8_1:-71938542 Msa1212090:three_prime_UTR
AAGACAAAATCAATGGCTGT+TGG 0.520940 8_1:+71938429 None:intergenic
TCAATGATTATGCACCATCT+GGG 0.530735 8_1:-71938720 Msa1212090:CDS
ATTTATGTATGATGAAAGAA+AGG 0.544291 8_1:-71938586 Msa1212090:three_prime_UTR
GCCAAGAATTAATGAGAGGA+TGG 0.546052 8_1:-71938752 Msa1212090:CDS
AAGATCCCATACATAAACAA+TGG 0.554557 8_1:+71938537 None:intergenic
ATATATAGGTGGACGGTAGA+GGG 0.556953 8_1:+71938315 None:intergenic
GTGTTACATGCAGGTTAAAG+TGG 0.557750 8_1:+71938391 None:intergenic
TAAATTGAGAACTAGCATAT+GGG 0.561490 8_1:-71939365 Msa1212090:five_prime_UTR
TACTCCGGAAAATACCCCTC+AGG 0.579089 8_1:-71938918 Msa1212090:intron
ACACTCCAAGAGCACCGTAA+TGG 0.580515 8_1:-71938684 Msa1212090:exon
TTAATGCTTGTGTTACATGC+AGG 0.582300 8_1:+71938382 None:intergenic
CCAAGTTGACCTAATTACTC+CGG 0.594810 8_1:-71938933 Msa1212090:CDS
CAAAGCCAAGAATTAATGAG+AGG 0.628754 8_1:-71938756 Msa1212090:CDS
TGACGACCATTTGCCCCAGA+TGG 0.633835 8_1:+71938706 None:intergenic
CAATGATTATGCACCATCTG+GGG 0.636327 8_1:-71938719 Msa1212090:CDS
TTAAGCATAGAAGATGTGAA+AGG 0.652061 8_1:+71939250 None:intergenic
ATTGAGAACTAGCATATGGG+AGG 0.656265 8_1:-71939362 Msa1212090:five_prime_UTR
GATCCCATACATAAACAATG+GGG 0.744720 8_1:+71938539 None:intergenic
AGTATCAAACTGTACCTGAG+GGG 0.746962 8_1:+71938904 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATAAGACAAAATCAA+TGG + chr8_1:71939319-71939338 None:intergenic 15.0%
!! ATAAAATAAGACAAAATCAA+TGG + chr8_1:71939319-71939338 None:intergenic 15.0%
!!! TTTGTGATGTTTTTTTAACA+TGG + chr8_1:71938336-71938355 None:intergenic 20.0%
!! AACAAGATTATATATGTTAC+AGG - chr8_1:71938926-71938945 Msa1212090:CDS 20.0%
!!! CTTAATATTGTTTTACATAG+AGG + chr8_1:71939105-71939124 None:intergenic 20.0%
!!! ATATATGAGTTTTATTGTAG+AGG - chr8_1:71939128-71939147 Msa1212090:intron 20.0%
!! ATTTATGTATGATGAAAGAA+AGG - chr8_1:71939152-71939171 Msa1212090:intron 20.0%
!!! TTTGTGATGTTTTTTTAACA+TGG + chr8_1:71938336-71938355 None:intergenic 20.0%
!! AACAAGATTATATATGTTAC+AGG - chr8_1:71938926-71938945 Msa1212090:CDS 20.0%
!!! CTTAATATTGTTTTACATAG+AGG + chr8_1:71939105-71939124 None:intergenic 20.0%
!!! ATATATGAGTTTTATTGTAG+AGG - chr8_1:71939128-71939147 Msa1212090:intron 20.0%
!! ATTTATGTATGATGAAAGAA+AGG - chr8_1:71939152-71939171 Msa1212090:intron 20.0%
! ATAAATTGAGAACTAGCATA+TGG - chr8_1:71938372-71938391 Msa1212090:three_prime_UTR 25.0%
! TAAATTGAGAACTAGCATAT+GGG - chr8_1:71938373-71938392 Msa1212090:three_prime_UTR 25.0%
! TAGAAGAATTTACTTGTAAC+TGG + chr8_1:71938522-71938541 None:intergenic 25.0%
! TACGTCTATATTTGAATTGA+CGG - chr8_1:71938574-71938593 Msa1212090:three_prime_UTR 25.0%
!! AGTCTTAGTCTTCTTATATA+TGG - chr8_1:71938739-71938758 Msa1212090:CDS 25.0%
! ATCATTGAAATTCTAGTACA+TGG + chr8_1:71939265-71939284 None:intergenic 25.0%
! ATTAAGCACAAAACTGAAAA+AGG - chr8_1:71939374-71939393 Msa1212090:five_prime_UTR 25.0%
! TTAAGCACAAAACTGAAAAA+GGG - chr8_1:71939375-71939394 Msa1212090:five_prime_UTR 25.0%
!! TTTTTGCACCAATATGAATA+TGG + chr8_1:71939402-71939421 None:intergenic 25.0%
! ATAAATTGAGAACTAGCATA+TGG - chr8_1:71938372-71938391 Msa1212090:three_prime_UTR 25.0%
! TAAATTGAGAACTAGCATAT+GGG - chr8_1:71938373-71938392 Msa1212090:three_prime_UTR 25.0%
! TAGAAGAATTTACTTGTAAC+TGG + chr8_1:71938522-71938541 None:intergenic 25.0%
! TACGTCTATATTTGAATTGA+CGG - chr8_1:71938574-71938593 Msa1212090:three_prime_UTR 25.0%
!! AGTCTTAGTCTTCTTATATA+TGG - chr8_1:71938739-71938758 Msa1212090:CDS 25.0%
! ATCATTGAAATTCTAGTACA+TGG + chr8_1:71939265-71939284 None:intergenic 25.0%
! ATTAAGCACAAAACTGAAAA+AGG - chr8_1:71939374-71939393 Msa1212090:five_prime_UTR 25.0%
! TTAAGCACAAAACTGAAAAA+GGG - chr8_1:71939375-71939394 Msa1212090:five_prime_UTR 25.0%
!! TTTTTGCACCAATATGAATA+TGG + chr8_1:71939402-71939421 None:intergenic 25.0%
TTAAGCATAGAAGATGTGAA+AGG + chr8_1:71938491-71938510 None:intergenic 30.0%
ATTTGAATTGACGGTGAATT+TGG - chr8_1:71938583-71938602 Msa1212090:three_prime_UTR 30.0%
TGTATTTCATGAAATCATGG+TGG + chr8_1:71938678-71938697 None:intergenic 30.0%
CGATGTATTTCATGAAATCA+TGG + chr8_1:71938681-71938700 None:intergenic 30.0%
GGAAAATTACATGCATGTTT+CGG + chr8_1:71938708-71938727 None:intergenic 30.0%
ATGGTATGTTTGTATTAAGC+AGG - chr8_1:71938758-71938777 Msa1212090:CDS 30.0%
TATTGAGAAAAACTTGCACT+TGG - chr8_1:71938953-71938972 Msa1212090:CDS 30.0%
! AATTAATGAGAGGATGGATT+TGG - chr8_1:71938992-71939011 Msa1212090:intron 30.0%
AGAGGAACTAACATTTAATC+AGG + chr8_1:71939087-71939106 None:intergenic 30.0%
AAATCCCCATTGTTTATGTA+TGG - chr8_1:71939195-71939214 Msa1212090:intron 30.0%
AATCCCCATTGTTTATGTAT+GGG - chr8_1:71939196-71939215 Msa1212090:intron 30.0%
AGATCCCATACATAAACAAT+GGG + chr8_1:71939203-71939222 None:intergenic 30.0%
AAGATCCCATACATAAACAA+TGG + chr8_1:71939204-71939223 None:intergenic 30.0%
TTGTTTATGTATGGGATCTT+AGG - chr8_1:71939204-71939223 Msa1212090:intron 30.0%
AAAAGGGTCCATATTCATAT+TGG - chr8_1:71939391-71939410 Msa1212090:five_prime_UTR 30.0%
! TTTTAACATGGAATGTCGAT+TGG + chr8_1:71938324-71938343 None:intergenic 30.0%
TTAAGCATAGAAGATGTGAA+AGG + chr8_1:71938491-71938510 None:intergenic 30.0%
ATTTGAATTGACGGTGAATT+TGG - chr8_1:71938583-71938602 Msa1212090:three_prime_UTR 30.0%
TGTATTTCATGAAATCATGG+TGG + chr8_1:71938678-71938697 None:intergenic 30.0%
CGATGTATTTCATGAAATCA+TGG + chr8_1:71938681-71938700 None:intergenic 30.0%
GGAAAATTACATGCATGTTT+CGG + chr8_1:71938708-71938727 None:intergenic 30.0%
ATGGTATGTTTGTATTAAGC+AGG - chr8_1:71938758-71938777 Msa1212090:CDS 30.0%
TATTGAGAAAAACTTGCACT+TGG - chr8_1:71938953-71938972 Msa1212090:CDS 30.0%
! AATTAATGAGAGGATGGATT+TGG - chr8_1:71938992-71939011 Msa1212090:intron 30.0%
AGAGGAACTAACATTTAATC+AGG + chr8_1:71939087-71939106 None:intergenic 30.0%
AAATCCCCATTGTTTATGTA+TGG - chr8_1:71939195-71939214 Msa1212090:intron 30.0%
AATCCCCATTGTTTATGTAT+GGG - chr8_1:71939196-71939215 Msa1212090:intron 30.0%
AGATCCCATACATAAACAAT+GGG + chr8_1:71939203-71939222 None:intergenic 30.0%
AAGATCCCATACATAAACAA+TGG + chr8_1:71939204-71939223 None:intergenic 30.0%
TTGTTTATGTATGGGATCTT+AGG - chr8_1:71939204-71939223 Msa1212090:intron 30.0%
AAAAGGGTCCATATTCATAT+TGG - chr8_1:71939391-71939410 Msa1212090:five_prime_UTR 30.0%
!!! CTAGAGCTTGTTTTTGAGAA+GGG - chr8_1:71938408-71938427 Msa1212090:three_prime_UTR 35.0%
CGTTTATTCGTGATTCTTCT+TGG - chr8_1:71938465-71938484 Msa1212090:three_prime_UTR 35.0%
GACTAAGACTAAGAAGATGA+AGG + chr8_1:71938729-71938748 None:intergenic 35.0%
CTCTCATTAATTCTTGGCTT+TGG + chr8_1:71938984-71939003 None:intergenic 35.0%
CAAAGCCAAGAATTAATGAG+AGG - chr8_1:71938982-71939001 Msa1212090:intron 35.0%
TCCATCCTCTCATTAATTCT+TGG + chr8_1:71938990-71939009 None:intergenic 35.0%
TCAATGATTATGCACCATCT+GGG - chr8_1:71939018-71939037 Msa1212090:intron 35.0%
AATCAGGTTACACACCATTA+CGG + chr8_1:71939071-71939090 None:intergenic 35.0%
GATCCCATACATAAACAATG+GGG + chr8_1:71939202-71939221 None:intergenic 35.0%
AAGACAAAATCAATGGCTGT+TGG + chr8_1:71939312-71939331 None:intergenic 35.0%
TTAATGCTTGTGTTACATGC+AGG + chr8_1:71939359-71939378 None:intergenic 35.0%
!!! CTAGAGCTTGTTTTTGAGAA+GGG - chr8_1:71938408-71938427 Msa1212090:three_prime_UTR 35.0%
CGTTTATTCGTGATTCTTCT+TGG - chr8_1:71938465-71938484 Msa1212090:three_prime_UTR 35.0%
GACTAAGACTAAGAAGATGA+AGG + chr8_1:71938729-71938748 None:intergenic 35.0%
CTCTCATTAATTCTTGGCTT+TGG + chr8_1:71938984-71939003 None:intergenic 35.0%
CAAAGCCAAGAATTAATGAG+AGG - chr8_1:71938982-71939001 Msa1212090:intron 35.0%
TCCATCCTCTCATTAATTCT+TGG + chr8_1:71938990-71939009 None:intergenic 35.0%
TCAATGATTATGCACCATCT+GGG - chr8_1:71939018-71939037 Msa1212090:intron 35.0%
AATCAGGTTACACACCATTA+CGG + chr8_1:71939071-71939090 None:intergenic 35.0%
GATCCCATACATAAACAATG+GGG + chr8_1:71939202-71939221 None:intergenic 35.0%
AAGACAAAATCAATGGCTGT+TGG + chr8_1:71939312-71939331 None:intergenic 35.0%
TTAATGCTTGTGTTACATGC+AGG + chr8_1:71939359-71939378 None:intergenic 35.0%
ATTGAGAACTAGCATATGGG+AGG - chr8_1:71938376-71938395 Msa1212090:three_prime_UTR 40.0%
CCTTCTCAAAAACAAGCTCT+AGG + chr8_1:71938410-71938429 None:intergenic 40.0%
!!! CCTAGAGCTTGTTTTTGAGA+AGG - chr8_1:71938407-71938426 Msa1212090:three_prime_UTR 40.0%
TGAATTGACGGTGAATTTGG+TGG - chr8_1:71938586-71938605 Msa1212090:three_prime_UTR 40.0%
CAACTTGGACATCTTGCATA+AGG + chr8_1:71938793-71938812 None:intergenic 40.0%
! CCAAGTTGACCTAATTACTC+CGG - chr8_1:71938805-71938824 Msa1212090:intron 40.0%
! GGGTATTTTCCGGAGTAATT+AGG + chr8_1:71938817-71938836 None:intergenic 40.0%
AGTATCAAACTGTACCTGAG+GGG + chr8_1:71938837-71938856 None:intergenic 40.0%
GAGTATCAAACTGTACCTGA+GGG + chr8_1:71938838-71938857 None:intergenic 40.0%
! AGAGTATCAAACTGTACCTG+AGG + chr8_1:71938839-71938858 None:intergenic 40.0%
GCCAAGAATTAATGAGAGGA+TGG - chr8_1:71938986-71939005 Msa1212090:intron 40.0%
CTCAATGATTATGCACCATC+TGG - chr8_1:71939017-71939036 Msa1212090:intron 40.0%
CAATGATTATGCACCATCTG+GGG - chr8_1:71939019-71939038 Msa1212090:intron 40.0%
GTGTTACATGCAGGTTAAAG+TGG + chr8_1:71939350-71939369 None:intergenic 40.0%
ATTGAGAACTAGCATATGGG+AGG - chr8_1:71938376-71938395 Msa1212090:three_prime_UTR 40.0%
CCTTCTCAAAAACAAGCTCT+AGG + chr8_1:71938410-71938429 None:intergenic 40.0%
!!! CCTAGAGCTTGTTTTTGAGA+AGG - chr8_1:71938407-71938426 Msa1212090:three_prime_UTR 40.0%
TGAATTGACGGTGAATTTGG+TGG - chr8_1:71938586-71938605 Msa1212090:three_prime_UTR 40.0%
CAACTTGGACATCTTGCATA+AGG + chr8_1:71938793-71938812 None:intergenic 40.0%
! CCAAGTTGACCTAATTACTC+CGG - chr8_1:71938805-71938824 Msa1212090:intron 40.0%
! GGGTATTTTCCGGAGTAATT+AGG + chr8_1:71938817-71938836 None:intergenic 40.0%
AGTATCAAACTGTACCTGAG+GGG + chr8_1:71938837-71938856 None:intergenic 40.0%
GAGTATCAAACTGTACCTGA+GGG + chr8_1:71938838-71938857 None:intergenic 40.0%
! AGAGTATCAAACTGTACCTG+AGG + chr8_1:71938839-71938858 None:intergenic 40.0%
GCCAAGAATTAATGAGAGGA+TGG - chr8_1:71938986-71939005 Msa1212090:intron 40.0%
CTCAATGATTATGCACCATC+TGG - chr8_1:71939017-71939036 Msa1212090:intron 40.0%
CAATGATTATGCACCATCTG+GGG - chr8_1:71939019-71939038 Msa1212090:intron 40.0%
GTGTTACATGCAGGTTAAAG+TGG + chr8_1:71939350-71939369 None:intergenic 40.0%
CCGGAGTAATTAGGTCAACT+TGG + chr8_1:71938808-71938827 None:intergenic 45.0%
!!! TGTACCTGAGGGGTATTTTC+CGG + chr8_1:71938827-71938846 None:intergenic 45.0%
CCGGAGTAATTAGGTCAACT+TGG + chr8_1:71938808-71938827 None:intergenic 45.0%
!!! TGTACCTGAGGGGTATTTTC+CGG + chr8_1:71938827-71938846 None:intergenic 45.0%
TACTCCGGAAAATACCCCTC+AGG - chr8_1:71938820-71938839 Msa1212090:intron 50.0%
! TATGCACCATCTGGGGCAAA+TGG - chr8_1:71939026-71939045 Msa1212090:intron 50.0%
!! ACACTCCAAGAGCACCGTAA+TGG - chr8_1:71939054-71939073 Msa1212090:intron 50.0%
ACACACCATTACGGTGCTCT+TGG + chr8_1:71939062-71939081 None:intergenic 50.0%
TACTCCGGAAAATACCCCTC+AGG - chr8_1:71938820-71938839 Msa1212090:intron 50.0%
! TATGCACCATCTGGGGCAAA+TGG - chr8_1:71939026-71939045 Msa1212090:intron 50.0%
!! ACACTCCAAGAGCACCGTAA+TGG - chr8_1:71939054-71939073 Msa1212090:intron 50.0%
ACACACCATTACGGTGCTCT+TGG + chr8_1:71939062-71939081 None:intergenic 50.0%
GAGAAGGGTCATTGCAGTGC+AGG - chr8_1:71938423-71938442 Msa1212090:three_prime_UTR 55.0%
GGGTCATTGCAGTGCAGGAA+TGG - chr8_1:71938428-71938447 Msa1212090:three_prime_UTR 55.0%
CATTGCAGTGCAGGAATGGC+AGG - chr8_1:71938432-71938451 Msa1212090:three_prime_UTR 55.0%
TGACGACCATTTGCCCCAGA+TGG + chr8_1:71939035-71939054 None:intergenic 55.0%
GAGAAGGGTCATTGCAGTGC+AGG - chr8_1:71938423-71938442 Msa1212090:three_prime_UTR 55.0%
GGGTCATTGCAGTGCAGGAA+TGG - chr8_1:71938428-71938447 Msa1212090:three_prime_UTR 55.0%
CATTGCAGTGCAGGAATGGC+AGG - chr8_1:71938432-71938451 Msa1212090:three_prime_UTR 55.0%
TGACGACCATTTGCCCCAGA+TGG + chr8_1:71939035-71939054 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 71938324 71939436 71938324 ID=Msa1212090;Name=Msa1212090
chr8_1 mRNA 71938324 71939436 71938324 ID=Msa1212090-mRNA-1;Parent=Msa1212090;Name=Msa1212090-mRNA-1;_AED=0.03;_eAED=0.03;_QI=123|1|1|1|1|1|3|363|89
chr8_1 exon 71938324 71938812 71938324 ID=Msa1212090-mRNA-1:exon:24252;Parent=Msa1212090-mRNA-1
chr8_1 exon 71938919 71938980 71938919 ID=Msa1212090-mRNA-1:exon:24251;Parent=Msa1212090-mRNA-1
chr8_1 exon 71939232 71939436 71939232 ID=Msa1212090-mRNA-1:exon:24250;Parent=Msa1212090-mRNA-1
chr8_1 five_prime_UTR 71939314 71939436 71939314 ID=Msa1212090-mRNA-1:five_prime_utr;Parent=Msa1212090-mRNA-1
chr8_1 CDS 71939232 71939313 71939232 ID=Msa1212090-mRNA-1:cds;Parent=Msa1212090-mRNA-1
chr8_1 CDS 71938919 71938980 71938919 ID=Msa1212090-mRNA-1:cds;Parent=Msa1212090-mRNA-1
chr8_1 CDS 71938687 71938812 71938687 ID=Msa1212090-mRNA-1:cds;Parent=Msa1212090-mRNA-1
chr8_1 three_prime_UTR 71938324 71938686 71938324 ID=Msa1212090-mRNA-1:three_prime_utr;Parent=Msa1212090-mRNA-1
Gene Sequence

>Msa1212090

ATGGCAGGAACTTTCTTTCGTTTATTCGTGATTCTTCTTGGCCTTTCACATCTTCTATGCTTAACAGCAGTTCCAGTTACAAGAACTGAAAACCTTATGCAAGATGTCCAAGTTGACCTAATTACTCCGGAAAATACCCCTCAGGTTATTATTGAGAAAAACTTGCACTTGGAAGAGCCAAAGCCAAGAATTAATGAGAGGATGGATTTGGAACTCAATGATTATGCACCATCTGGGGCAAATGGTCGTCACACTCCAAGAGCACCGTAA

Protein sequence

>Msa1212090

MAGTFFRLFVILLGLSHLLCLTAVPVTRTENLMQDVQVDLITPENTPQVIIEKNLHLEEPKPRINERMDLELNDYAPSGANGRHTPRAP*