AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0660140


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0660140 MtrunA17_Chr8g0385371 98.438 192 3 0 1 192 1 192 4.67e-138 383
Msa0660140 MtrunA17_Chr4g0047141 34.054 185 94 6 20 188 61 233 6.31e-13 65.1
Msa0660140 MtrunA17_Chr2g0282361 34.826 201 93 10 7 188 71 252 4.87e-12 62.8
Msa0660140 MtrunA17_Chr2g0317321 32.683 205 91 9 14 190 58 243 8.07e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0660140 AT5G08480.2 56.983 179 55 6 16 191 13 172 6.74e-46 149
Msa0660140 AT5G08480.3 56.983 179 55 6 16 191 13 172 6.74e-46 149
Msa0660140 AT5G08480.1 47.486 179 41 5 16 191 13 141 7.45e-31 110
Msa0660140 AT5G53830.1 32.828 198 95 5 20 186 49 239 4.34e-12 63.2
Msa0660140 AT1G28280.1 33.810 210 87 11 11 188 49 238 8.86e-11 59.7
Msa0660140 AT1G28280.2 33.810 210 87 11 11 188 49 238 8.89e-11 59.7

Find 49 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACAAACACAGGTGCATTT+AGG 0.187922 4_3:-70703125 Msa0660140:CDS
GTTCTCTCAGTTAACTATTA+TGG 0.217006 4_3:-70702821 Msa0660140:CDS
TTCCTTCTTTCATGGAGTTT+TGG 0.237618 4_3:+70703009 None:intergenic
GGAAACAAGTTAAGCAATTC+TGG 0.246716 4_3:+70702673 None:intergenic
TCATGCATTTCCTTCTTTCA+TGG 0.261349 4_3:+70703001 None:intergenic
GGTGGTGTTCTCTTCGTAAT+AGG 0.264699 4_3:+70703030 None:intergenic
GGTTTAACAATCTCAAGTTT+TGG 0.319524 4_3:+70702976 None:intergenic
CACAAACACAGGTGCATTTA+GGG 0.321361 4_3:-70703124 Msa0660140:CDS
TTTCCTTCAAGTCCTGTATC+AGG 0.338299 4_3:-70702892 Msa0660140:CDS
GAAGTAGTGCAAAGATTAAC+AGG 0.361566 4_3:-70703102 Msa0660140:CDS
TGTTTGTGATATTGAAGATT+TGG 0.376002 4_3:+70702955 None:intergenic
TAGGTGCAACCTTTGTAGTT+TGG 0.379631 4_3:+70703049 None:intergenic
GTGTGAACAAATGTTGTTAG+TGG 0.401194 4_3:+70703144 None:intergenic
CTTTGTTGCATTGCCTTCTG+AGG 0.427730 4_3:+70703077 None:intergenic
CCAGTACATGGTTCAACAAC+AGG 0.437636 4_3:-70703177 Msa0660140:CDS
CATACTTTCTCACATGCCTT+AGG 0.439034 4_3:+70702640 None:intergenic
TTTGTTGCATTGCCTTCTGA+GGG 0.457769 4_3:+70703078 None:intergenic
GTTTCCACTGGCATCTCCTA+AGG 0.476906 4_3:-70702656 Msa0660140:CDS
GAATTTCTTGACTTAGAAGA+AGG 0.481290 4_3:+70702919 None:intergenic
CTTGCTTTAGACCTGGGTGA+TGG 0.493410 4_3:+70702709 None:intergenic
GAACTTCCACTACCTGATAC+AGG 0.493636 4_3:+70702880 None:intergenic
GTGGAAGGTGTGGTAGGGCT+TGG 0.499380 4_3:+70702847 None:intergenic
CTACCTGATACAGGACTTGA+AGG 0.504395 4_3:+70702889 None:intergenic
ATTGCTTAACTTGTTTCCAC+TGG 0.509456 4_3:-70702668 Msa0660140:CDS
GAAGCCACTTGCTTTAGACC+TGG 0.517471 4_3:+70702702 None:intergenic
TCAAGTCCTGTATCAGGTAG+TGG 0.519505 4_3:-70702886 Msa0660140:CDS
AAGCCACTTGCTTTAGACCT+GGG 0.539510 4_3:+70702703 None:intergenic
CACCAAAACTCCATGAAAGA+AGG 0.543366 4_3:-70703011 Msa0660140:CDS
CTCAGAAGGCAATGCAACAA+AGG 0.546327 4_3:-70703076 Msa0660140:CDS
ATGGAGAAGCCACCAGTACA+TGG 0.555074 4_3:-70703189 Msa0660140:CDS
CCTGTTGTTGAACCATGTAC+TGG 0.565708 4_3:+70703177 None:intergenic
AACAATGTGGAAGGTGTGGT+AGG 0.566267 4_3:+70702841 None:intergenic
AAATCTGATTGTGATGGGAA+TGG 0.567485 4_3:-70703208 None:intergenic
AGATTAACAGGTCCCTCAGA+AGG 0.568541 4_3:-70703090 Msa0660140:CDS
ACAATGTGGAAGGTGTGGTA+GGG 0.605585 4_3:+70702842 None:intergenic
GTTGTTGAACCATGTACTGG+TGG 0.616693 4_3:+70703180 None:intergenic
TGGAAGATGAAAAGAAACAA+GGG 0.617549 4_3:-70702801 Msa0660140:CDS
ACATTTGTTCACACAAACAC+AGG 0.619702 4_3:-70703135 Msa0660140:CDS
ATGGAAGATGAAAAGAAACA+AGG 0.623994 4_3:-70702802 Msa0660140:CDS
AACTGAGAGAACAATGTGGA+AGG 0.625030 4_3:+70702832 None:intergenic
CTTCAATATCACAAACAACC+AGG 0.631097 4_3:-70702949 Msa0660140:CDS
TCACCCAGGTCTAAAGCAAG+TGG 0.631290 4_3:-70702706 Msa0660140:CDS
AGAAAGCTATCAAAGAGAGA+CGG 0.633848 4_3:-70702744 Msa0660140:CDS
TTTATTTGCATCCATCACCC+AGG 0.643004 4_3:-70702720 Msa0660140:CDS
TTAGAAGAAGGTGATGCACC+TGG 0.665275 4_3:+70702931 None:intergenic
AAAGGAAGACCAAACTACAA+AGG 0.666842 4_3:-70703058 Msa0660140:CDS
AGAGAACAATGTGGAAGGTG+TGG 0.689227 4_3:+70702837 None:intergenic
CATGCCTTAGGAGATGCCAG+TGG 0.723253 4_3:+70702652 None:intergenic
AGTTAACTGAGAGAACAATG+TGG 0.758270 4_3:+70702828 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGTTTGTGATATTGAAGATT+TGG + chr4_3:70702882-70702901 None:intergenic 25.0%
! GGTTTAACAATCTCAAGTTT+TGG + chr4_3:70702861-70702880 None:intergenic 30.0%
GAATTTCTTGACTTAGAAGA+AGG + chr4_3:70702918-70702937 None:intergenic 30.0%
GTTCTCTCAGTTAACTATTA+TGG - chr4_3:70703013-70703032 Msa0660140:CDS 30.0%
ATGGAAGATGAAAAGAAACA+AGG - chr4_3:70703032-70703051 Msa0660140:CDS 30.0%
TGGAAGATGAAAAGAAACAA+GGG - chr4_3:70703033-70703052 Msa0660140:CDS 30.0%
GTGTGAACAAATGTTGTTAG+TGG + chr4_3:70702693-70702712 None:intergenic 35.0%
ACATTTGTTCACACAAACAC+AGG - chr4_3:70702699-70702718 Msa0660140:CDS 35.0%
GAAGTAGTGCAAAGATTAAC+AGG - chr4_3:70702732-70702751 Msa0660140:CDS 35.0%
AAAGGAAGACCAAACTACAA+AGG - chr4_3:70702776-70702795 Msa0660140:CDS 35.0%
! TTCCTTCTTTCATGGAGTTT+TGG + chr4_3:70702828-70702847 None:intergenic 35.0%
TCATGCATTTCCTTCTTTCA+TGG + chr4_3:70702836-70702855 None:intergenic 35.0%
CTTCAATATCACAAACAACC+AGG - chr4_3:70702885-70702904 Msa0660140:CDS 35.0%
AGTTAACTGAGAGAACAATG+TGG + chr4_3:70703009-70703028 None:intergenic 35.0%
AGAAAGCTATCAAAGAGAGA+CGG - chr4_3:70703090-70703109 Msa0660140:CDS 35.0%
GGAAACAAGTTAAGCAATTC+TGG + chr4_3:70703164-70703183 None:intergenic 35.0%
!! ATTGCTTAACTTGTTTCCAC+TGG - chr4_3:70703166-70703185 Msa0660140:CDS 35.0%
ACACAAACACAGGTGCATTT+AGG - chr4_3:70702709-70702728 Msa0660140:CDS 40.0%
CACAAACACAGGTGCATTTA+GGG - chr4_3:70702710-70702729 Msa0660140:CDS 40.0%
TTTGTTGCATTGCCTTCTGA+GGG + chr4_3:70702759-70702778 None:intergenic 40.0%
TAGGTGCAACCTTTGTAGTT+TGG + chr4_3:70702788-70702807 None:intergenic 40.0%
!! CTTCTTTCATGGAGTTTTGG+TGG + chr4_3:70702825-70702844 None:intergenic 40.0%
CACCAAAACTCCATGAAAGA+AGG - chr4_3:70702823-70702842 Msa0660140:CDS 40.0%
TTTCCTTCAAGTCCTGTATC+AGG - chr4_3:70702942-70702961 Msa0660140:CDS 40.0%
AACTGAGAGAACAATGTGGA+AGG + chr4_3:70703005-70703024 None:intergenic 40.0%
TTTATTTGCATCCATCACCC+AGG - chr4_3:70703114-70703133 Msa0660140:CDS 40.0%
CATACTTTCTCACATGCCTT+AGG + chr4_3:70703197-70703216 None:intergenic 40.0%
GTTGTTGAACCATGTACTGG+TGG + chr4_3:70702657-70702676 None:intergenic 45.0%
CCTGTTGTTGAACCATGTAC+TGG + chr4_3:70702660-70702679 None:intergenic 45.0%
CCAGTACATGGTTCAACAAC+AGG - chr4_3:70702657-70702676 Msa0660140:CDS 45.0%
AGATTAACAGGTCCCTCAGA+AGG - chr4_3:70702744-70702763 Msa0660140:CDS 45.0%
CTTTGTTGCATTGCCTTCTG+AGG + chr4_3:70702760-70702779 None:intergenic 45.0%
CTCAGAAGGCAATGCAACAA+AGG - chr4_3:70702758-70702777 Msa0660140:CDS 45.0%
GGTGGTGTTCTCTTCGTAAT+AGG + chr4_3:70702807-70702826 None:intergenic 45.0%
TTAGAAGAAGGTGATGCACC+TGG + chr4_3:70702906-70702925 None:intergenic 45.0%
CTACCTGATACAGGACTTGA+AGG + chr4_3:70702948-70702967 None:intergenic 45.0%
TCAAGTCCTGTATCAGGTAG+TGG - chr4_3:70702948-70702967 Msa0660140:CDS 45.0%
GAACTTCCACTACCTGATAC+AGG + chr4_3:70702957-70702976 None:intergenic 45.0%
! ACAATGTGGAAGGTGTGGTA+GGG + chr4_3:70702995-70703014 None:intergenic 45.0%
! AACAATGTGGAAGGTGTGGT+AGG + chr4_3:70702996-70703015 None:intergenic 45.0%
AGAGAACAATGTGGAAGGTG+TGG + chr4_3:70703000-70703019 None:intergenic 45.0%
! AAGCCACTTGCTTTAGACCT+GGG + chr4_3:70703134-70703153 None:intergenic 45.0%
ATGGAGAAGCCACCAGTACA+TGG - chr4_3:70702645-70702664 Msa0660140:CDS 50.0%
! CTTGCTTTAGACCTGGGTGA+TGG + chr4_3:70703128-70703147 None:intergenic 50.0%
! TCACCCAGGTCTAAAGCAAG+TGG - chr4_3:70703128-70703147 Msa0660140:CDS 50.0%
! GAAGCCACTTGCTTTAGACC+TGG + chr4_3:70703135-70703154 None:intergenic 50.0%
GTTTCCACTGGCATCTCCTA+AGG - chr4_3:70703178-70703197 Msa0660140:CDS 50.0%
CATGCCTTAGGAGATGCCAG+TGG + chr4_3:70703185-70703204 None:intergenic 55.0%
! GTGGAAGGTGTGGTAGGGCT+TGG + chr4_3:70702990-70703009 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 70702639 70703217 70702639 ID=Msa0660140;Name=Msa0660140
chr4_3 mRNA 70702639 70703217 70702639 ID=Msa0660140-mRNA-1;Parent=Msa0660140;Name=Msa0660140-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|-1|0|1|-1|1|1|0|192
chr4_3 exon 70702639 70703217 70702639 ID=Msa0660140-mRNA-1:exon:21111;Parent=Msa0660140-mRNA-1
chr4_3 CDS 70702639 70703217 70702639 ID=Msa0660140-mRNA-1:cds;Parent=Msa0660140-mRNA-1
Gene Sequence

>Msa0660140

ATGGGAATGGAGAAGCCACCAGTACATGGTTCAACAACAGGTGATTGTAAACCACTAACAACATTTGTTCACACAAACACAGGTGCATTTAGGGAAGTAGTGCAAAGATTAACAGGTCCCTCAGAAGGCAATGCAACAAAGGAAGACCAAACTACAAAGGTTGCACCTATTACGAAGAGAACACCACCAAAACTCCATGAAAGAAGGAAATGCATGAAGCCAAAACTTGAGATTGTTAAACCAAATCTTCAATATCACAAACAACCAGGTGCATCACCTTCTTCTAAGTCAAGAAATTCTAGCTTTCCTTCAAGTCCTGTATCAGGTAGTGGAAGTTCTAGTCTTCTTCCAAGCCCTACCACACCTTCCACATTGTTCTCTCAGTTAACTATTATGGAAGATGAAAAGAAACAAGGGTCTGTGATAAATGAAATAAACATAGAAGAAGAAGAGAAAGCTATCAAAGAGAGACGGTTTTATTTGCATCCATCACCCAGGTCTAAAGCAAGTGGCTTCAATGAACCAGAATTGCTTAACTTGTTTCCACTGGCATCTCCTAAGGCATGTGAGAAAGTATGA

Protein sequence

>Msa0660140

MGMEKPPVHGSTTGDCKPLTTFVHTNTGAFREVVQRLTGPSEGNATKEDQTTKVAPITKRTPPKLHERRKCMKPKLEIVKPNLQYHKQPGASPSSKSRNSSFPSSPVSGSGSSSLLPSPTTPSTLFSQLTIMEDEKKQGSVINEINIEEEEKAIKERRFYLHPSPRSKASGFNEPELLNLFPLASPKACEKV*