AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0541700


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0541700 MtrunA17_Chr8g0385371 98.438 192 3 0 1 192 1 192 4.67e-138 383
Msa0541700 MtrunA17_Chr4g0047141 34.054 185 94 6 20 188 61 233 6.31e-13 65.1
Msa0541700 MtrunA17_Chr2g0282361 34.826 201 93 10 7 188 71 252 4.87e-12 62.8
Msa0541700 MtrunA17_Chr2g0317321 32.683 205 91 9 14 190 58 243 8.07e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0541700 AT5G08480.2 56.983 179 55 6 16 191 13 172 6.74e-46 149
Msa0541700 AT5G08480.3 56.983 179 55 6 16 191 13 172 6.74e-46 149
Msa0541700 AT5G08480.1 47.486 179 41 5 16 191 13 141 7.45e-31 110
Msa0541700 AT5G53830.1 32.828 198 95 5 20 186 49 239 4.34e-12 63.2
Msa0541700 AT1G28280.1 33.810 210 87 11 11 188 49 238 8.86e-11 59.7
Msa0541700 AT1G28280.2 33.810 210 87 11 11 188 49 238 8.89e-11 59.7

Find 49 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACAAACACAGGTGCATTT+AGG 0.187922 4_1:-25730923 Msa0541700:CDS
GTTCTCTCAGTTAACTATTA+TGG 0.217006 4_1:-25730619 Msa0541700:CDS
TTCCTTCTTTCATGGAGTTT+TGG 0.237618 4_1:+25730807 None:intergenic
GGAAACAAGTTAAGCAATTC+TGG 0.246716 4_1:+25730471 None:intergenic
TCATGCATTTCCTTCTTTCA+TGG 0.261349 4_1:+25730799 None:intergenic
GGTGGTGTTCTCTTCGTAAT+AGG 0.264699 4_1:+25730828 None:intergenic
GGTTTAACAATCTCAAGTTT+TGG 0.319524 4_1:+25730774 None:intergenic
CACAAACACAGGTGCATTTA+GGG 0.321361 4_1:-25730922 Msa0541700:CDS
TTTCCTTCAAGTCCTGTATC+AGG 0.338299 4_1:-25730690 Msa0541700:CDS
GAAGTAGTGCAAAGATTAAC+AGG 0.361566 4_1:-25730900 Msa0541700:CDS
TGTTTGTGATATTGAAGATT+TGG 0.376002 4_1:+25730753 None:intergenic
TAGGTGCAACCTTTGTAGTT+TGG 0.379631 4_1:+25730847 None:intergenic
GTGTGAACAAATGTTGTTAG+TGG 0.401194 4_1:+25730942 None:intergenic
CTTTGTTGCATTGCCTTCTG+AGG 0.427730 4_1:+25730875 None:intergenic
CCAGTACATGGTTCAACAAC+AGG 0.437636 4_1:-25730975 Msa0541700:CDS
CATACTTTCTCACATGCCTT+AGG 0.439034 4_1:+25730438 None:intergenic
TTTGTTGCATTGCCTTCTGA+GGG 0.457769 4_1:+25730876 None:intergenic
GTTTCCACTGGCATCTCCTA+AGG 0.476906 4_1:-25730454 Msa0541700:CDS
GAATTTCTTGACTTAGAAGA+AGG 0.481290 4_1:+25730717 None:intergenic
CTTGCTTTAGACCTGGGTGA+TGG 0.493410 4_1:+25730507 None:intergenic
GAACTTCCACTACCTGATAC+AGG 0.493636 4_1:+25730678 None:intergenic
GTGGAAGGTGTGGTAGGGCT+TGG 0.499380 4_1:+25730645 None:intergenic
CTACCTGATACAGGACTTGA+AGG 0.504395 4_1:+25730687 None:intergenic
ATTGCTTAACTTGTTTCCAC+TGG 0.509456 4_1:-25730466 Msa0541700:CDS
GAAGCCACTTGCTTTAGACC+TGG 0.517471 4_1:+25730500 None:intergenic
TCAAGTCCTGTATCAGGTAG+TGG 0.519505 4_1:-25730684 Msa0541700:CDS
AAGCCACTTGCTTTAGACCT+GGG 0.539510 4_1:+25730501 None:intergenic
CACCAAAACTCCATGAAAGA+AGG 0.543366 4_1:-25730809 Msa0541700:CDS
CTCAGAAGGCAATGCAACAA+AGG 0.546327 4_1:-25730874 Msa0541700:CDS
ATGGAGAAGCCACCAGTACA+TGG 0.555074 4_1:-25730987 Msa0541700:CDS
CCTGTTGTTGAACCATGTAC+TGG 0.565708 4_1:+25730975 None:intergenic
AACAATGTGGAAGGTGTGGT+AGG 0.566267 4_1:+25730639 None:intergenic
AAATCTGATTGTGATGGGAA+TGG 0.567485 4_1:-25731006 None:intergenic
AGATTAACAGGTCCCTCAGA+AGG 0.568541 4_1:-25730888 Msa0541700:CDS
ACAATGTGGAAGGTGTGGTA+GGG 0.605585 4_1:+25730640 None:intergenic
GTTGTTGAACCATGTACTGG+TGG 0.616693 4_1:+25730978 None:intergenic
TGGAAGATGAAAAGAAACAA+GGG 0.617549 4_1:-25730599 Msa0541700:CDS
ACATTTGTTCACACAAACAC+AGG 0.619702 4_1:-25730933 Msa0541700:CDS
ATGGAAGATGAAAAGAAACA+AGG 0.623994 4_1:-25730600 Msa0541700:CDS
AACTGAGAGAACAATGTGGA+AGG 0.625030 4_1:+25730630 None:intergenic
CTTCAATATCACAAACAACC+AGG 0.631097 4_1:-25730747 Msa0541700:CDS
TCACCCAGGTCTAAAGCAAG+TGG 0.631290 4_1:-25730504 Msa0541700:CDS
AGAAAGCTATCAAAGAGAGA+CGG 0.633848 4_1:-25730542 Msa0541700:CDS
TTTATTTGCATCCATCACCC+AGG 0.643004 4_1:-25730518 Msa0541700:CDS
TTAGAAGAAGGTGATGCACC+TGG 0.665275 4_1:+25730729 None:intergenic
AAAGGAAGACCAAACTACAA+AGG 0.666842 4_1:-25730856 Msa0541700:CDS
AGAGAACAATGTGGAAGGTG+TGG 0.689227 4_1:+25730635 None:intergenic
CATGCCTTAGGAGATGCCAG+TGG 0.723253 4_1:+25730450 None:intergenic
AGTTAACTGAGAGAACAATG+TGG 0.758270 4_1:+25730626 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGTTTGTGATATTGAAGATT+TGG + chr4_1:25730680-25730699 None:intergenic 25.0%
! GGTTTAACAATCTCAAGTTT+TGG + chr4_1:25730659-25730678 None:intergenic 30.0%
GAATTTCTTGACTTAGAAGA+AGG + chr4_1:25730716-25730735 None:intergenic 30.0%
GTTCTCTCAGTTAACTATTA+TGG - chr4_1:25730811-25730830 Msa0541700:CDS 30.0%
ATGGAAGATGAAAAGAAACA+AGG - chr4_1:25730830-25730849 Msa0541700:CDS 30.0%
TGGAAGATGAAAAGAAACAA+GGG - chr4_1:25730831-25730850 Msa0541700:CDS 30.0%
GTGTGAACAAATGTTGTTAG+TGG + chr4_1:25730491-25730510 None:intergenic 35.0%
ACATTTGTTCACACAAACAC+AGG - chr4_1:25730497-25730516 Msa0541700:CDS 35.0%
GAAGTAGTGCAAAGATTAAC+AGG - chr4_1:25730530-25730549 Msa0541700:CDS 35.0%
AAAGGAAGACCAAACTACAA+AGG - chr4_1:25730574-25730593 Msa0541700:CDS 35.0%
! TTCCTTCTTTCATGGAGTTT+TGG + chr4_1:25730626-25730645 None:intergenic 35.0%
TCATGCATTTCCTTCTTTCA+TGG + chr4_1:25730634-25730653 None:intergenic 35.0%
CTTCAATATCACAAACAACC+AGG - chr4_1:25730683-25730702 Msa0541700:CDS 35.0%
AGTTAACTGAGAGAACAATG+TGG + chr4_1:25730807-25730826 None:intergenic 35.0%
AGAAAGCTATCAAAGAGAGA+CGG - chr4_1:25730888-25730907 Msa0541700:CDS 35.0%
GGAAACAAGTTAAGCAATTC+TGG + chr4_1:25730962-25730981 None:intergenic 35.0%
!! ATTGCTTAACTTGTTTCCAC+TGG - chr4_1:25730964-25730983 Msa0541700:CDS 35.0%
ACACAAACACAGGTGCATTT+AGG - chr4_1:25730507-25730526 Msa0541700:CDS 40.0%
CACAAACACAGGTGCATTTA+GGG - chr4_1:25730508-25730527 Msa0541700:CDS 40.0%
TTTGTTGCATTGCCTTCTGA+GGG + chr4_1:25730557-25730576 None:intergenic 40.0%
TAGGTGCAACCTTTGTAGTT+TGG + chr4_1:25730586-25730605 None:intergenic 40.0%
!! CTTCTTTCATGGAGTTTTGG+TGG + chr4_1:25730623-25730642 None:intergenic 40.0%
CACCAAAACTCCATGAAAGA+AGG - chr4_1:25730621-25730640 Msa0541700:CDS 40.0%
TTTCCTTCAAGTCCTGTATC+AGG - chr4_1:25730740-25730759 Msa0541700:CDS 40.0%
AACTGAGAGAACAATGTGGA+AGG + chr4_1:25730803-25730822 None:intergenic 40.0%
TTTATTTGCATCCATCACCC+AGG - chr4_1:25730912-25730931 Msa0541700:CDS 40.0%
CATACTTTCTCACATGCCTT+AGG + chr4_1:25730995-25731014 None:intergenic 40.0%
GTTGTTGAACCATGTACTGG+TGG + chr4_1:25730455-25730474 None:intergenic 45.0%
CCTGTTGTTGAACCATGTAC+TGG + chr4_1:25730458-25730477 None:intergenic 45.0%
CCAGTACATGGTTCAACAAC+AGG - chr4_1:25730455-25730474 Msa0541700:CDS 45.0%
AGATTAACAGGTCCCTCAGA+AGG - chr4_1:25730542-25730561 Msa0541700:CDS 45.0%
CTTTGTTGCATTGCCTTCTG+AGG + chr4_1:25730558-25730577 None:intergenic 45.0%
CTCAGAAGGCAATGCAACAA+AGG - chr4_1:25730556-25730575 Msa0541700:CDS 45.0%
GGTGGTGTTCTCTTCGTAAT+AGG + chr4_1:25730605-25730624 None:intergenic 45.0%
TTAGAAGAAGGTGATGCACC+TGG + chr4_1:25730704-25730723 None:intergenic 45.0%
CTACCTGATACAGGACTTGA+AGG + chr4_1:25730746-25730765 None:intergenic 45.0%
TCAAGTCCTGTATCAGGTAG+TGG - chr4_1:25730746-25730765 Msa0541700:CDS 45.0%
GAACTTCCACTACCTGATAC+AGG + chr4_1:25730755-25730774 None:intergenic 45.0%
! ACAATGTGGAAGGTGTGGTA+GGG + chr4_1:25730793-25730812 None:intergenic 45.0%
! AACAATGTGGAAGGTGTGGT+AGG + chr4_1:25730794-25730813 None:intergenic 45.0%
AGAGAACAATGTGGAAGGTG+TGG + chr4_1:25730798-25730817 None:intergenic 45.0%
! AAGCCACTTGCTTTAGACCT+GGG + chr4_1:25730932-25730951 None:intergenic 45.0%
ATGGAGAAGCCACCAGTACA+TGG - chr4_1:25730443-25730462 Msa0541700:CDS 50.0%
! CTTGCTTTAGACCTGGGTGA+TGG + chr4_1:25730926-25730945 None:intergenic 50.0%
! TCACCCAGGTCTAAAGCAAG+TGG - chr4_1:25730926-25730945 Msa0541700:CDS 50.0%
! GAAGCCACTTGCTTTAGACC+TGG + chr4_1:25730933-25730952 None:intergenic 50.0%
GTTTCCACTGGCATCTCCTA+AGG - chr4_1:25730976-25730995 Msa0541700:CDS 50.0%
CATGCCTTAGGAGATGCCAG+TGG + chr4_1:25730983-25731002 None:intergenic 55.0%
! GTGGAAGGTGTGGTAGGGCT+TGG + chr4_1:25730788-25730807 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 25730437 25731015 25730437 ID=Msa0541700;Name=Msa0541700
chr4_1 mRNA 25730437 25731015 25730437 ID=Msa0541700-mRNA-1;Parent=Msa0541700;Name=Msa0541700-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|-1|0|1|-1|1|1|0|192
chr4_1 exon 25730437 25731015 25730437 ID=Msa0541700-mRNA-1:exon:6988;Parent=Msa0541700-mRNA-1
chr4_1 CDS 25730437 25731015 25730437 ID=Msa0541700-mRNA-1:cds;Parent=Msa0541700-mRNA-1
Gene Sequence

>Msa0541700

ATGGGAATGGAGAAGCCACCAGTACATGGTTCAACAACAGGTGATTGTAAACCACTAACAACATTTGTTCACACAAACACAGGTGCATTTAGGGAAGTAGTGCAAAGATTAACAGGTCCCTCAGAAGGCAATGCAACAAAGGAAGACCAAACTACAAAGGTTGCACCTATTACGAAGAGAACACCACCAAAACTCCATGAAAGAAGGAAATGCATGAAGCCAAAACTTGAGATTGTTAAACCAAATCTTCAATATCACAAACAACCAGGTGCATCACCTTCTTCTAAGTCAAGAAATTCTAGCTTTCCTTCAAGTCCTGTATCAGGTAGTGGAAGTTCTAGTCTTCTTCCAAGCCCTACCACACCTTCCACATTGTTCTCTCAGTTAACTATTATGGAAGATGAAAAGAAACAAGGGTCTGTGATAAATGAAATAAACATAGAAGAAGAAGAGAAAGCTATCAAAGAGAGACGGTTTTATTTGCATCCATCACCCAGGTCTAAAGCAAGTGGCTTCAATGAACCAGAATTGCTTAACTTGTTTCCACTGGCATCTCCTAAGGCATGTGAGAAAGTATGA

Protein sequence

>Msa0541700

MGMEKPPVHGSTTGDCKPLTTFVHTNTGAFREVVQRLTGPSEGNATKEDQTTKVAPITKRTPPKLHERRKCMKPKLEIVKPNLQYHKQPGASPSSKSRNSSFPSSPVSGSGSSSLLPSPTTPSTLFSQLTIMEDEKKQGSVINEINIEEEEKAIKERRFYLHPSPRSKASGFNEPELLNLFPLASPKACEKV*