AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1437740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1437740 MtrunA17_Chr8g0382231 98.986 493 5 0 1 493 138 630 0.0 1021
Msa1437740 MtrunA17_Chr4g0050021 43.694 563 225 9 1 493 129 669 3.46e-146 434
Msa1437740 MtrunA17_Chr2g0279481 42.959 561 226 11 1 486 129 670 1.94e-140 419
Msa1437740 MtrunA17_Chr2g0303541 32.308 520 278 8 1 470 112 607 1.84e-87 281
Msa1437740 MtrunA17_Chr1g0191711 34.932 438 240 8 1 433 147 544 6.21e-84 269
Msa1437740 MtrunA17_Chr5g0401451 35.153 458 237 8 1 429 125 551 7.55e-84 271
Msa1437740 MtrunA17_Chr1g0181181 34.867 413 228 8 1 396 113 501 2.09e-78 255
Msa1437740 MtrunA17_Chr8g0374011 35.681 426 200 8 1 381 125 521 3.24e-78 257
Msa1437740 MtrunA17_Chr1g0195911 33.265 487 237 11 1 434 121 572 4.65e-78 256
Msa1437740 MtrunA17_Chr2g0285511 32.448 527 272 14 1 485 113 597 7.42e-78 255
Msa1437740 MtrunA17_Chr7g0268781 32.948 519 259 12 1 465 121 604 9.63e-77 253
Msa1437740 MtrunA17_Chr5g0401341 32.558 473 223 11 1 415 111 545 1.95e-76 252
Msa1437740 MtrunA17_Chr4g0010091 34.644 407 209 6 1 379 119 496 2.97e-76 251
Msa1437740 MtrunA17_Chr2g0320691 29.495 495 275 9 1 443 110 582 3.10e-75 249
Msa1437740 MtrunA17_Chr1g0150191 32.280 443 218 9 1 398 112 517 3.59e-74 245
Msa1437740 MtrunA17_Chr1g0199631 30.680 515 263 12 1 463 76 548 4.02e-74 244
Msa1437740 MtrunA17_Chr4g0053841 34.216 453 234 9 1 422 100 519 4.83e-73 242
Msa1437740 MtrunA17_Chr8g0367891 35.949 395 210 6 1 374 112 484 1.05e-72 241
Msa1437740 MtrunA17_Chr1g0207461 32.347 473 223 14 1 415 113 546 2.49e-72 241
Msa1437740 MtrunA17_Chr7g0245201 35.396 404 208 8 1 379 147 522 5.81e-71 237
Msa1437740 MtrunA17_Chr3g0108331 30.478 502 298 11 1 493 109 568 4.90e-69 231
Msa1437740 MtrunA17_Chr5g0412591 33.747 403 194 6 42 391 124 506 1.97e-68 230
Msa1437740 MtrunA17_Chr4g0003891 37.209 301 154 5 78 375 46 314 3.36e-67 223
Msa1437740 MtrunA17_Chr4g0076521 34.351 393 211 7 1 374 111 475 1.73e-66 224
Msa1437740 MtrunA17_Chr2g0332281 37.793 299 157 5 78 375 24 294 3.92e-64 214
Msa1437740 MtrunA17_Chr5g0432861 31.019 432 266 5 1 424 110 517 1.45e-63 216
Msa1437740 MtrunA17_Chr2g0290741 31.325 415 221 9 1 395 111 481 1.53e-58 202
Msa1437740 MtrunA17_Chr4g0037661 25.367 477 249 11 1 422 93 517 1.88e-36 141
Msa1437740 MtrunA17_Chr4g0023801 23.961 409 222 10 1 376 92 444 8.46e-30 122
Msa1437740 MtrunA17_Chr1g0163311 23.077 494 269 13 1 437 86 525 6.71e-29 119
Msa1437740 MtrunA17_Chr2g0280541 22.248 427 259 11 3 405 24 401 1.11e-24 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1437740 AT5G10250.2 58.000 500 190 7 1 493 136 622 0.0 553
Msa1437740 AT5G10250.1 56.600 500 182 7 1 493 136 607 0.0 533
Msa1437740 AT5G64330.1 38.119 606 216 9 1 469 137 720 7.15e-129 392
Msa1437740 AT5G64330.3 41.296 494 155 6 1 379 78 551 1.25e-122 371
Msa1437740 AT5G64330.2 41.296 494 155 6 1 379 137 610 9.71e-122 371
Msa1437740 AT5G67385.4 36.776 397 201 7 1 377 66 432 6.66e-80 259
Msa1437740 AT5G67385.5 36.776 397 201 7 1 377 66 432 6.66e-80 259
Msa1437740 AT5G67385.2 36.776 397 201 7 1 377 66 432 6.66e-80 259
Msa1437740 AT5G67385.3 36.776 397 201 7 1 377 66 432 6.66e-80 259
Msa1437740 AT5G67385.1 36.776 397 201 7 1 377 111 477 1.36e-79 259
Msa1437740 AT5G13600.1 32.129 526 265 14 1 478 110 591 4.12e-78 255
Msa1437740 AT5G03250.2 32.617 512 255 14 1 462 1 472 5.17e-77 249
Msa1437740 AT1G30440.1 33.649 422 216 5 1 379 113 513 7.86e-77 254
Msa1437740 AT5G48800.1 33.985 409 195 4 1 379 126 489 5.32e-76 250
Msa1437740 AT5G03250.1 32.417 509 260 13 1 462 113 584 7.35e-76 249
Msa1437740 AT5G67440.2 35.859 396 198 8 1 374 78 439 2.32e-75 247
Msa1437740 AT1G03010.2 33.081 529 254 14 1 463 91 585 5.99e-75 248
Msa1437740 AT1G03010.1 33.081 529 254 14 1 463 118 612 7.78e-75 248
Msa1437740 AT5G67440.3 35.859 396 198 8 1 374 115 476 8.83e-75 246
Msa1437740 AT5G67440.1 35.859 396 198 8 1 374 115 476 8.83e-75 246
Msa1437740 AT1G03010.3 33.081 529 254 14 1 463 117 611 1.30e-74 247
Msa1437740 AT2G47860.5 32.179 491 239 9 1 434 1 454 3.94e-73 240
Msa1437740 AT2G47860.2 32.179 491 239 9 1 434 1 454 3.94e-73 240
Msa1437740 AT2G47860.1 32.179 491 239 9 1 434 119 572 2.83e-72 241
Msa1437740 AT3G44820.1 31.301 492 261 11 1 434 111 583 2.88e-72 241
Msa1437740 AT2G47860.3 32.179 491 239 9 1 434 142 595 4.11e-72 241
Msa1437740 AT1G52770.1 33.417 398 214 8 60 435 10 378 9.47e-72 235
Msa1437740 AT2G14820.2 33.255 424 206 8 1 384 112 498 1.52e-71 239
Msa1437740 AT2G14820.1 33.255 424 206 8 1 384 112 498 1.52e-71 239
Msa1437740 AT3G08570.1 32.189 466 240 9 1 424 121 552 7.08e-70 234
Msa1437740 AT5G66560.4 33.810 420 205 9 1 379 109 496 8.99e-69 232
Msa1437740 AT5G66560.3 33.810 420 205 9 1 379 119 506 1.49e-68 231
Msa1437740 AT1G67900.5 32.653 441 209 7 1 383 108 518 1.86e-68 231
Msa1437740 AT1G67900.1 32.653 441 209 7 1 383 108 518 1.86e-68 231
Msa1437740 AT1G67900.3 32.653 441 209 7 1 383 108 518 1.86e-68 231
Msa1437740 AT1G67900.6 32.653 441 209 7 1 383 108 518 1.86e-68 231
Msa1437740 AT1G67900.2 32.653 441 209 7 1 383 108 518 1.86e-68 231
Msa1437740 AT1G67900.4 32.653 441 209 7 1 383 108 518 1.86e-68 231
Msa1437740 AT3G08660.3 30.162 494 270 10 1 468 58 502 2.65e-68 228
Msa1437740 AT5G66560.1 33.810 420 205 9 1 379 148 535 2.78e-68 231
Msa1437740 AT2G30520.2 33.079 393 224 7 1 383 67 430 4.89e-68 228
Msa1437740 AT2G30520.3 33.079 393 224 7 1 383 67 430 4.89e-68 228
Msa1437740 AT3G08660.1 30.162 494 270 10 1 468 118 562 1.13e-67 228
Msa1437740 AT2G30520.1 33.079 393 224 7 1 383 115 478 1.21e-67 228
Msa1437740 AT3G50840.2 29.960 504 292 12 1 482 66 530 1.09e-66 224
Msa1437740 AT3G50840.3 29.960 504 292 12 1 482 66 530 1.09e-66 224
Msa1437740 AT4G37590.1 31.448 442 227 11 1 415 123 515 1.44e-66 224
Msa1437740 AT3G50840.1 29.960 504 292 12 1 482 102 566 4.23e-66 223
Msa1437740 AT3G49970.1 35.180 361 188 8 1 359 78 394 4.00e-64 216
Msa1437740 AT3G49970.2 35.180 361 188 8 1 359 78 394 4.00e-64 216
Msa1437740 AT4G31820.2 32.518 409 189 11 1 374 59 415 1.19e-63 215
Msa1437740 AT4G31820.1 32.518 409 189 11 1 374 112 468 3.08e-63 216
Msa1437740 AT3G26490.1 31.603 443 193 9 1 378 114 511 4.68e-63 216
Msa1437740 AT3G26490.3 31.603 443 193 9 1 378 114 511 4.68e-63 216
Msa1437740 AT3G26490.4 31.603 443 193 9 1 378 114 511 4.68e-63 216
Msa1437740 AT3G15570.1 35.599 309 160 5 78 375 29 309 2.37e-62 210
Msa1437740 AT5G47800.3 31.899 395 215 9 1 372 111 474 6.37e-62 212
Msa1437740 AT5G47800.1 31.899 395 215 9 1 372 111 474 6.37e-62 212
Msa1437740 AT5G47800.4 31.899 395 215 9 1 372 111 474 6.37e-62 212
Msa1437740 AT5G47800.2 31.899 395 215 9 1 372 224 587 5.16e-61 212
Msa1437740 AT2G23050.2 30.380 395 201 8 1 378 1 338 2.97e-57 194
Msa1437740 AT2G23050.1 30.380 395 201 8 1 378 117 454 5.43e-56 194
Msa1437740 AT2G47860.6 32.044 362 172 7 1 324 142 467 2.08e-47 171
Msa1437740 AT2G47860.4 32.044 362 172 7 1 324 119 444 2.40e-47 170
Msa1437740 AT3G08660.2 29.799 349 184 6 1 331 118 423 1.71e-43 159
Msa1437740 AT3G26490.2 29.306 389 165 9 1 324 114 457 1.15e-42 159
Msa1437740 AT5G66560.2 30.411 365 181 9 1 324 148 480 2.46e-42 157
Msa1437740 AT3G19850.1 27.470 415 214 9 1 373 95 464 5.02e-40 152
Msa1437740 AT5G48130.1 24.570 407 231 9 1 381 120 476 2.14e-34 137
Msa1437740 AT1G50280.2 25.845 414 234 8 1 381 62 435 7.89e-34 134
Msa1437740 AT1G50280.1 25.845 414 234 8 1 381 95 468 1.01e-33 134
Msa1437740 AT5G17580.2 26.382 398 229 10 1 376 104 459 1.16e-29 122
Msa1437740 AT3G03510.1 26.905 420 238 13 1 412 39 397 1.81e-29 120
Msa1437740 AT5G17580.1 26.382 398 229 10 1 376 89 444 2.04e-29 122
Msa1437740 AT3G22104.4 26.198 313 172 7 48 359 91 345 2.16e-24 105
Msa1437740 AT3G22104.2 26.198 313 172 7 48 359 91 345 2.16e-24 105
Msa1437740 AT3G22104.3 26.198 313 172 7 48 359 91 345 2.16e-24 105
Msa1437740 AT3G22104.1 25.879 313 173 6 48 359 166 420 3.33e-24 105

Find 135 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTGTCAGACTGACTTTC+AGG 0.132980 tig0028361:-8123 None:intergenic
CACAGCATCTTCTAACAATT+TGG 0.179939 tig0028361:-9406 None:intergenic
CTCCAGCCTGATAGATTCTT+TGG 0.216318 tig0028361:-11726 None:intergenic
GAGGAGGAATTATGCTTATT+AGG 0.225056 tig0028361:-9760 None:intergenic
ATTCAATATTAGTAGGCTTT+TGG 0.241298 tig0028361:+10251 Msa1437740:CDS
CATTTGTCGTGCTTTCTTCA+TGG 0.250887 tig0028361:+9324 Msa1437740:CDS
CAGCCTCTAATCTCAAATTC+TGG 0.250919 tig0028361:+9110 Msa1437740:five_prime_UTR
TCTGGTTTCATATATCATTT+AGG 0.251520 tig0028361:+8008 Msa1437740:five_prime_UTR
TTAGATATAATATAAAATTT+AGG 0.251980 tig0028361:-11947 None:intergenic
TGCGGATAATTTCAGCAATT+AGG 0.253941 tig0028361:+9540 Msa1437740:CDS
AACATTGTAATTGAAAGATT+TGG 0.262115 tig0028361:-10303 None:intergenic
TTCCCAGAATTTGAGATTAG+AGG 0.277466 tig0028361:-9113 None:intergenic
AAGCTGGTGGTTCGATGATT+TGG 0.289085 tig0028361:+9493 Msa1437740:CDS
GACTTTCAGGGTCTTGTGCT+TGG 0.289224 tig0028361:-8110 None:intergenic
GCGGATAATTTCAGCAATTA+GGG 0.291903 tig0028361:+9541 Msa1437740:CDS
TTAGAAGGGCTTAGAGGATA+TGG 0.292438 tig0028361:+9644 Msa1437740:CDS
CTCAAGCTTGAAAACTTTCC+AGG 0.304064 tig0028361:+9140 Msa1437740:five_prime_UTR
TATCAGGCTGGAGTTTAAAT+TGG 0.321879 tig0028361:+11736 Msa1437740:CDS
GCACATTATAAGTGGTTTCC+TGG 0.326824 tig0028361:-8423 None:intergenic
TGTCTATAGTTTCAGATGTC+TGG 0.334509 tig0028361:-11593 None:intergenic
ACCTGGACAACTGTTCTTAG+AGG 0.338185 tig0028361:-10620 None:intergenic
ACTTTCAGGGTCTTGTGCTT+GGG 0.351122 tig0028361:-8109 None:intergenic
CCTACTAATATTGAATTTCC+CGG 0.364748 tig0028361:-10244 None:intergenic
ACAGTACTCAGCATTAATTT+AGG 0.366320 tig0028361:+7978 Msa1437740:five_prime_UTR
GAGGAGTTAGAAGGGCTTAG+AGG 0.370662 tig0028361:+9638 Msa1437740:CDS
AAATTTAGGCTATATGCTTC+AGG 0.374622 tig0028361:-11933 None:intergenic
ACAATTCTAAAATTCTGTTA+TGG 0.376842 tig0028361:+9182 Msa1437740:five_prime_UTR
TGCAGAGTCTTCTACGGTCT+TGG 0.377991 tig0028361:-10532 None:intergenic
TTCTACGGTCTTGGTCAGTT+AGG 0.381517 tig0028361:-10523 None:intergenic
CCGGGAAATTCAATATTAGT+AGG 0.395757 tig0028361:+10244 Msa1437740:CDS
TTTACCAATGATCTCTGGTT+TGG 0.398294 tig0028361:-9573 None:intergenic
GATGCTGCATCCTCACTTGC+GGG 0.402710 tig0028361:-9461 None:intergenic
AGCTATTGTGACATGTTTGT+TGG 0.410370 tig0028361:-8177 None:intergenic
GACAAACCAAAGAATCTATC+AGG 0.413587 tig0028361:+11720 Msa1437740:CDS
ACACGGAGAGCCTTCTTCTC+GGG 0.418325 tig0028361:-11860 None:intergenic
GAGCTAATTGCTGCAAGCTT+GGG 0.418641 tig0028361:-8057 None:intergenic
TCTAACTCCTCATACATTCC+TGG 0.422868 tig0028361:-9626 None:intergenic
GACCTTTAGGTAGGTGTCAA+TGG 0.427446 tig0028361:-10388 None:intergenic
TTAATTCCTAGATATCAAAA+TGG 0.429080 tig0028361:+9920 Msa1437740:CDS
AGCCTCTAATCTCAAATTCT+GGG 0.431924 tig0028361:+9111 Msa1437740:five_prime_UTR
ATGCAACTGCAACAAAAGTC+CGG 0.435207 tig0028361:+10225 Msa1437740:CDS
ATTAATTTAGGCAGCGTGTC+TGG 0.435511 tig0028361:+7990 Msa1437740:five_prime_UTR
GACACGGAGAGCCTTCTTCT+CGG 0.443418 tig0028361:-11861 None:intergenic
TTATCCGAGTTGAAGTCTAT+AGG 0.445995 tig0028361:-9209 None:intergenic
TATCCGAGTTGAAGTCTATA+GGG 0.446756 tig0028361:-9208 None:intergenic
CTTGAATTGAAAAGTCTGTT+GGG 0.449484 tig0028361:-8399 None:intergenic
TTCTGTCAGACTGACTTTCA+GGG 0.449988 tig0028361:-8122 None:intergenic
TCTACGGTCTTGGTCAGTTA+GGG 0.449995 tig0028361:-10522 None:intergenic
TCCTTCCATGTAAAACCAAC+AGG 0.451380 tig0028361:+11774 Msa1437740:CDS
ATACATTTACCAATGATCTC+TGG 0.452219 tig0028361:-9578 None:intergenic
AAGTGGAATCAACTCTCAAC+AGG 0.453660 tig0028361:+11563 Msa1437740:CDS
TGAGCTAATTGCTGCAAGCT+TGG 0.454672 tig0028361:-8058 None:intergenic
TCTCTAGTTTCACCTCCTGT+TGG 0.456759 tig0028361:-11789 None:intergenic
TACCTTGTGCACATTATAAG+TGG 0.465167 tig0028361:-8431 None:intergenic
GGAATGTATGAGGAGTTAGA+AGG 0.465676 tig0028361:+9629 Msa1437740:CDS
CTTGGTCAGTTAGGGAAGGA+TGG 0.466551 tig0028361:-10514 None:intergenic
TGAAATGATCGAGTATGTTC+TGG 0.476508 tig0028361:-10348 None:intergenic
ACTTGAATTGAAAAGTCTGT+TGG 0.476835 tig0028361:-8400 None:intergenic
ATTTCAGCAATTAGGGCAAA+GGG 0.478066 tig0028361:+9548 Msa1437740:CDS
ATATCAAAATGCGGCTACAT+AGG 0.478687 tig0028361:+9074 Msa1437740:five_prime_UTR
ATCTGCAACAAGAACTTACA+TGG 0.493501 tig0028361:-9797 None:intergenic
GCCTCTAAGAACAGTTGTCC+AGG 0.494899 tig0028361:+10619 Msa1437740:CDS
ACGAGTGTGGTTTGGTGATG+TGG 0.497567 tig0028361:-11826 None:intergenic
GATGCTGTGATTCTATAGCT+TGG 0.503818 tig0028361:+9420 Msa1437740:CDS
TGTGAGGAAAGCTTCAGTCT+TGG 0.507277 tig0028361:-9303 None:intergenic
GGAACCAAACCAGAGATCAT+TGG 0.508422 tig0028361:+9569 Msa1437740:CDS
GATCTACGACGAGTGTGGTT+TGG 0.513023 tig0028361:-11834 None:intergenic
CTTGTTGCAGATGCTAAAGA+TGG 0.513993 tig0028361:+9808 Msa1437740:CDS
GAAAAGTCTGTTGGGATTCG+AGG 0.514401 tig0028361:-8391 None:intergenic
AGCAATGCAAGAGAAGGAAC+CGG 0.515645 tig0028361:+11536 Msa1437740:CDS
CATTCCTGGTAGCCATCTCT+TGG 0.516344 tig0028361:-9612 None:intergenic
CTGTGATTCTATAGCTTGGA+AGG 0.517789 tig0028361:+9424 Msa1437740:CDS
TCAGACCTTGAAAAGAGAGT+TGG 0.522254 tig0028361:+9863 Msa1437740:CDS
GTGTAGAATTGTAGTTAAGA+TGG 0.523176 tig0028361:-7945 None:intergenic
CAGGCTGGAGTTTAAATTGG+CGG 0.523456 tig0028361:+11739 Msa1437740:CDS
TAGAAGGGCTTAGAGGATAT+GGG 0.526409 tig0028361:+9645 Msa1437740:CDS
GTAGATCAGCCCCGAGAAGA+AGG 0.527618 tig0028361:+11850 Msa1437740:CDS
CTTGTGAAAATCTATCTCCA+TGG 0.528028 tig0028361:+9372 Msa1437740:CDS
CCTTGAAAAGAGAGTTGGTA+TGG 0.528846 tig0028361:+9868 Msa1437740:CDS
AACCAAAGAATCTATCAGGC+TGG 0.531250 tig0028361:+11724 Msa1437740:CDS
AGATATCAAAATGGAGGTCA+AGG 0.533332 tig0028361:+9929 Msa1437740:CDS
TGCAACTGCAACAAAAGTCC+GGG 0.536493 tig0028361:+10226 Msa1437740:CDS
TTGGTCAGTTAGGGAAGGAT+GGG 0.536654 tig0028361:-10513 None:intergenic
AATTTCAGCAATTAGGGCAA+AGG 0.537477 tig0028361:+9547 Msa1437740:CDS
AAAAGTCTGTTGGGATTCGA+GGG 0.537749 tig0028361:-8390 None:intergenic
AAACATGAAATCACTTCAAG+TGG 0.548709 tig0028361:+8086 Msa1437740:five_prime_UTR
AGATGCTGCATCCTCACTTG+CGG 0.555801 tig0028361:-9462 None:intergenic
TTGTGAAAATCTATCTCCAT+GGG 0.559597 tig0028361:+9373 Msa1437740:CDS
TAAAAGGCTGACCTTTAGGT+AGG 0.562453 tig0028361:-10397 None:intergenic
GAGAGCAGCAATGCAAGAGA+AGG 0.563979 tig0028361:+11530 Msa1437740:CDS
TATGCCAAGAGATGGCTACC+AGG 0.565928 tig0028361:+9608 Msa1437740:CDS
ATGACTGAAGAACTAGAAGA+TGG 0.572238 tig0028361:+9269 Msa1437740:CDS
GATCTGTTGCAACTTCAGCT+AGG 0.573474 tig0028361:-10281 None:intergenic
TAGAATTGTAGTTAAGATGG+TGG 0.574829 tig0028361:-7942 None:intergenic
CAGCATCTAATCAAGAAAGC+TGG 0.575400 tig0028361:+9477 Msa1437740:CDS
GTTCTGCAGTTCTACCATGT+TGG 0.579053 tig0028361:-11652 None:intergenic
GAGTTGATTCCACTTTGTGC+CGG 0.581941 tig0028361:-11555 None:intergenic
CACGGAGAGCCTTCTTCTCG+GGG 0.584375 tig0028361:-11859 None:intergenic
ATGGCTACCAGGAATGTATG+AGG 0.585069 tig0028361:+9619 Msa1437740:CDS
TCAAATGTTTCACATCCACC+TGG 0.585855 tig0028361:-9158 None:intergenic
AGCCATTGACACCTACCTAA+AGG 0.587167 tig0028361:+10386 Msa1437740:CDS
TCATGCAATATGCCAAGAGA+TGG 0.594644 tig0028361:+9600 Msa1437740:CDS
CATGGGACAGCATCTTGTTG+AGG 0.601532 tig0028361:-9779 None:intergenic
AGAGAGCACTAGACATAGCA+AGG 0.603854 tig0028361:+9715 Msa1437740:CDS
CGAGTGTGGTTTGGTGATGT+GGG 0.605082 tig0028361:-11825 None:intergenic
GGGCTTAGAGGATATGGGCA+TGG 0.605115 tig0028361:+9650 Msa1437740:CDS
GAATGTATGAGGAGTTAGAA+GGG 0.605950 tig0028361:+9630 Msa1437740:CDS
CGGTCTTGGTCAGTTAGGGA+AGG 0.612259 tig0028361:-10518 None:intergenic
CGAGAATGTAAAGTCCAACA+TGG 0.615865 tig0028361:+11638 Msa1437740:CDS
CATAAAATGATCAATGCGGA+GGG 0.617020 tig0028361:-9519 None:intergenic
GGGACAGCATCTTGTTGAGG+AGG 0.618679 tig0028361:-9776 None:intergenic
GAGAAGGAACCGGCACAAAG+TGG 0.620698 tig0028361:+11546 Msa1437740:CDS
ATTCCTAGATATCAAAATGG+AGG 0.624406 tig0028361:+9923 Msa1437740:CDS
AAAATGATCAATGCGGAGGG+TGG 0.627548 tig0028361:-9516 None:intergenic
CAAAATGCGGCTACATAGGA+CGG 0.632260 tig0028361:+9078 Msa1437740:five_prime_UTR
AGAATTGTAGTTAAGATGGT+GGG 0.638014 tig0028361:-7941 None:intergenic
ATGCGGCTACATAGGACGGT+CGG 0.639956 tig0028361:+9082 Msa1437740:five_prime_UTR
GCATAAAATGATCAATGCGG+AGG 0.642568 tig0028361:-9520 None:intergenic
ACAGGAGGTGAAACTAGAGA+TGG 0.647018 tig0028361:+11792 Msa1437740:CDS
AAGAAAGCACGACAAATGTG+AGG 0.659530 tig0028361:-9319 None:intergenic
AAGCTTGAAAACTTTCCAGG+TGG 0.660259 tig0028361:+9143 Msa1437740:five_prime_UTR
CATCTAATCAAGAAAGCTGG+TGG 0.660897 tig0028361:+9480 Msa1437740:CDS
AACCACTTATAATGTGCACA+AGG 0.667560 tig0028361:+8429 Msa1437740:five_prime_UTR
CCTCCCTATAGACTTCAACT+CGG 0.675286 tig0028361:+9205 Msa1437740:five_prime_UTR
GGGCTGATCTACGACGAGTG+TGG 0.675575 tig0028361:-11839 None:intergenic
TAATGCTGAGTACTGTTCTG+TGG 0.676472 tig0028361:-7971 None:intergenic
CCGAGTTGAAGTCTATAGGG+AGG 0.687521 tig0028361:-9205 None:intergenic
TCTGCAACAAGAACTTACAT+GGG 0.692050 tig0028361:-9796 None:intergenic
AAAGTCTGTTGGGATTCGAG+GGG 0.692715 tig0028361:-8389 None:intergenic
TTCCATGTAAAACCAACAGG+AGG 0.698959 tig0028361:+11777 Msa1437740:CDS
TACCCCTTGATATCAAAATG+CGG 0.703247 tig0028361:+9065 Msa1437740:five_prime_UTR
TCCGCATAAAATGATCAATG+CGG 0.719094 tig0028361:-9523 None:intergenic
TATGGTGTTAGAAGATGCTG+AGG 0.726855 tig0028361:+9886 Msa1437740:CDS
CATACTCGATCATTTCACGA+TGG 0.744904 tig0028361:+10354 Msa1437740:CDS
AAGACATAGATGTAGTACAA+CGG 0.746121 tig0028361:+10166 Msa1437740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCAATTATCTTATTAAAAA+TGG - tig0028361:11307-11326 None:intergenic 10.0%
!! TTTAACTGCATAAAATATAA+AGG - tig0028361:8248-8267 None:intergenic 15.0%
!!! TTTATTTTCTCTGTTTTTTT+TGG + tig0028361:8343-8362 Msa1437740:intron 15.0%
!!! TTTATATTTTTCCATTTGTA+AGG - tig0028361:11008-11027 None:intergenic 15.0%
!!! ATATAAAGGATTCTATTTCT+GGG - tig0028361:8234-8253 None:intergenic 20.0%
!! AATATAAAGGATTCTATTTC+TGG - tig0028361:8235-8254 None:intergenic 20.0%
!! ACTCATACAAAAATAACTAT+TGG - tig0028361:8321-8340 None:intergenic 20.0%
!!! AATGATTTTCTGCATTTATA+AGG - tig0028361:8475-8494 None:intergenic 20.0%
!!! TGGTTTTCAAAAAAATTCTA+AGG - tig0028361:8930-8949 None:intergenic 20.0%
!! CAACATATAAAAGATTAAGT+TGG - tig0028361:9026-9045 None:intergenic 20.0%
!!! CAACTTAATCTTTTATATGT+TGG + tig0028361:9024-9043 Msa1437740:intron 20.0%
!!! ACAATTCTAAAATTCTGTTA+TGG + tig0028361:9182-9201 Msa1437740:five_prime_UTR 20.0%
!! TTAATTCCTAGATATCAAAA+TGG + tig0028361:9920-9939 Msa1437740:CDS 20.0%
!! TGCGAAAAATAAAAAATAAC+CGG + tig0028361:10037-10056 Msa1437740:intron 20.0%
!! AACATTGTAATTGAAAGATT+TGG - tig0028361:10306-10325 None:intergenic 20.0%
!! ACAAGAAAATAGGAATTAAA+AGG - tig0028361:10416-10435 None:intergenic 20.0%
!!! GATTTCTTTTTCAAAGTTAA+AGG - tig0028361:10881-10900 None:intergenic 20.0%
!!! TTTTATTTCTAAATCACAGT+AGG - tig0028361:11166-11185 None:intergenic 20.0%
!! TATCTTATTAAAAATGGAGT+TGG - tig0028361:11301-11320 None:intergenic 20.0%
!!! GATGAAATAATTTTAAGACA+CGG - tig0028361:11880-11899 None:intergenic 20.0%
!! TCTGGTTTCATATATCATTT+AGG + tig0028361:8008-8027 Msa1437740:five_prime_UTR 25.0%
! GCATAAAAAATGAACATTCA+AGG + tig0028361:8204-8223 Msa1437740:five_prime_UTR 25.0%
!!! TTTCTCTGTTTTTTTTGGTT+TGG + tig0028361:8348-8367 Msa1437740:intron 25.0%
!!! TTCTCTGTTTTTTTTGGTTT+GGG + tig0028361:8349-8368 Msa1437740:intron 25.0%
!!! CCATGTTTTTATTCTAATTC+TGG - tig0028361:8950-8969 None:intergenic 25.0%
! CCAGAATTAGAATAAAAACA+TGG + tig0028361:8947-8966 Msa1437740:intron 25.0%
!!! TTTTTTATTTTTCGCAGAGT+TGG - tig0028361:10033-10052 None:intergenic 25.0%
! GAAAAATAAAAAATAACCGG+AGG + tig0028361:10040-10059 Msa1437740:intron 25.0%
! AAGATAGAAATTAGTGTCTT+TGG - tig0028361:10079-10098 None:intergenic 25.0%
!! ATTCAATATTAGTAGGCTTT+TGG + tig0028361:10251-10270 Msa1437740:CDS 25.0%
! TGAATTGAACACAAGAAAAT+AGG - tig0028361:10426-10445 None:intergenic 25.0%
!! TATTTTCTTGTGTTCAATTC+AGG + tig0028361:10425-10444 Msa1437740:intron 25.0%
! GGAATTCTTAAAACTCTATT+TGG - tig0028361:10702-10721 None:intergenic 25.0%
!!! TGTCATCATTTTTTTCTTCA+TGG - tig0028361:10723-10742 None:intergenic 25.0%
! AAAGAAGAAACTTCTTTAGA+AGG + tig0028361:10944-10963 Msa1437740:intron 25.0%
AGAATTGTAGTTAAGATGGT+GGG - tig0028361:7944-7963 None:intergenic 30.0%
TAGAATTGTAGTTAAGATGG+TGG - tig0028361:7945-7964 None:intergenic 30.0%
GTGTAGAATTGTAGTTAAGA+TGG - tig0028361:7948-7967 None:intergenic 30.0%
ACAGTACTCAGCATTAATTT+AGG + tig0028361:7978-7997 Msa1437740:five_prime_UTR 30.0%
AAACATGAAATCACTTCAAG+TGG + tig0028361:8086-8105 Msa1437740:five_prime_UTR 30.0%
CTTGAATTGAAAAGTCTGTT+GGG - tig0028361:8402-8421 None:intergenic 30.0%
ACTTGAATTGAAAAGTCTGT+TGG - tig0028361:8403-8422 None:intergenic 30.0%
! TTTTACTGAGCTAAACAAGA+AGG - tig0028361:8500-8519 None:intergenic 30.0%
TTGTGAAAATCTATCTCCAT+GGG + tig0028361:9373-9392 Msa1437740:CDS 30.0%
ATACATTTACCAATGATCTC+TGG - tig0028361:9581-9600 None:intergenic 30.0%
ATTCCTAGATATCAAAATGG+AGG + tig0028361:9923-9942 Msa1437740:CDS 30.0%
!! TCATTTTTCTCCTGTCATAT+TGG + tig0028361:9980-9999 Msa1437740:intron 30.0%
AAGACATAGATGTAGTACAA+CGG + tig0028361:10166-10185 Msa1437740:CDS 30.0%
CCTACTAATATTGAATTTCC+CGG - tig0028361:10247-10266 None:intergenic 30.0%
!! TTATTTTGCAGAGTCTTCTA+CGG - tig0028361:10541-10560 None:intergenic 30.0%
!! TGTGTTGATGTGTTTTTCAA+AGG - tig0028361:10790-10809 None:intergenic 30.0%
ATTGAAGATCTGAACCAATT+TGG + tig0028361:10810-10829 Msa1437740:intron 30.0%
TTGAAGATCTGAACCAATTT+GGG + tig0028361:10811-10830 Msa1437740:intron 30.0%
! AAACTTCTTTAGAAGGAAAG+AGG + tig0028361:10951-10970 Msa1437740:intron 30.0%
! AACTTCTTTAGAAGGAAAGA+GGG + tig0028361:10952-10971 Msa1437740:intron 30.0%
GTACAAATGATCCTTACAAA+TGG + tig0028361:10994-11013 Msa1437740:intron 30.0%
! ACTCTATGACACATGATTTT+GGG - tig0028361:11072-11091 None:intergenic 30.0%
TGTATAGAGGAAATACTTTG+CGG - tig0028361:11121-11140 None:intergenic 30.0%
! ACACAAACTTTTGTGTATAG+AGG - tig0028361:11134-11153 None:intergenic 30.0%
AATTCCACAAAACAACATTG+TGG + tig0028361:11325-11344 Msa1437740:intron 30.0%
AAATTTAGGCTATATGCTTC+AGG - tig0028361:11936-11955 None:intergenic 30.0%
AGCTATTGTGACATGTTTGT+TGG - tig0028361:8180-8199 None:intergenic 35.0%
! AGACTTTTCAATTCAAGTCC+AGG + tig0028361:8405-8424 Msa1437740:five_prime_UTR 35.0%
AACCACTTATAATGTGCACA+AGG + tig0028361:8429-8448 Msa1437740:five_prime_UTR 35.0%
TACCTTGTGCACATTATAAG+TGG - tig0028361:8434-8453 None:intergenic 35.0%
! AAGAGCACAATCCTGTTAAT+AGG + tig0028361:8627-8646 Msa1437740:intron 35.0%
AGAGCACAATCCTGTTAATA+GGG + tig0028361:8628-8647 Msa1437740:intron 35.0%
GATTTATGCTCCCTATTAAC+AGG - tig0028361:8641-8660 None:intergenic 35.0%
! CTCTTTTGGATAGAGAAAGA+GGG + tig0028361:8740-8759 Msa1437740:intron 35.0%
! TCTTTTGGATAGAGAAAGAG+GGG + tig0028361:8741-8760 Msa1437740:intron 35.0%
!! TGTTTTGCATGCTTTTAAGG+AGG - tig0028361:8820-8839 None:intergenic 35.0%
!!! TGCTGTTTTGCATGCTTTTA+AGG - tig0028361:8823-8842 None:intergenic 35.0%
TACCCCTTGATATCAAAATG+CGG + tig0028361:9065-9084 Msa1437740:five_prime_UTR 35.0%
!! TAGCCGCATTTTGATATCAA+GGG - tig0028361:9071-9090 None:intergenic 35.0%
ATATCAAAATGCGGCTACAT+AGG + tig0028361:9074-9093 Msa1437740:five_prime_UTR 35.0%
AGCCTCTAATCTCAAATTCT+GGG + tig0028361:9111-9130 Msa1437740:five_prime_UTR 35.0%
! TTCCCAGAATTTGAGATTAG+AGG - tig0028361:9116-9135 None:intergenic 35.0%
! TATCCGAGTTGAAGTCTATA+GGG - tig0028361:9211-9230 None:intergenic 35.0%
! TTATCCGAGTTGAAGTCTAT+AGG - tig0028361:9212-9231 None:intergenic 35.0%
ATGACTGAAGAACTAGAAGA+TGG + tig0028361:9269-9288 Msa1437740:CDS 35.0%
CTTGTGAAAATCTATCTCCA+TGG + tig0028361:9372-9391 Msa1437740:CDS 35.0%
! ATTTGGATATTTTCAGCCCA+TGG - tig0028361:9392-9411 None:intergenic 35.0%
CACAGCATCTTCTAACAATT+TGG - tig0028361:9409-9428 None:intergenic 35.0%
CATAAAATGATCAATGCGGA+GGG - tig0028361:9522-9541 None:intergenic 35.0%
!! TCCGCATTGATCATTTTATG+CGG + tig0028361:9522-9541 Msa1437740:CDS 35.0%
TCCGCATAAAATGATCAATG+CGG - tig0028361:9526-9545 None:intergenic 35.0%
TGCGGATAATTTCAGCAATT+AGG + tig0028361:9540-9559 Msa1437740:CDS 35.0%
GCGGATAATTTCAGCAATTA+GGG + tig0028361:9541-9560 Msa1437740:CDS 35.0%
AATTTCAGCAATTAGGGCAA+AGG + tig0028361:9547-9566 Msa1437740:CDS 35.0%
ATTTCAGCAATTAGGGCAAA+GGG + tig0028361:9548-9567 Msa1437740:CDS 35.0%
TTTACCAATGATCTCTGGTT+TGG - tig0028361:9576-9595 None:intergenic 35.0%
GAATGTATGAGGAGTTAGAA+GGG + tig0028361:9630-9649 Msa1437740:CDS 35.0%
GAGGAGGAATTATGCTTATT+AGG - tig0028361:9763-9782 None:intergenic 35.0%
TCTGCAACAAGAACTTACAT+GGG - tig0028361:9799-9818 None:intergenic 35.0%
ATCTGCAACAAGAACTTACA+TGG - tig0028361:9800-9819 None:intergenic 35.0%
!! TGACCTCCATTTTGATATCT+AGG - tig0028361:9929-9948 None:intergenic 35.0%
AGATATCAAAATGGAGGTCA+AGG + tig0028361:9929-9948 Msa1437740:CDS 35.0%
AAATGGAGGTCAAGGAAAAA+TGG + tig0028361:9937-9956 Msa1437740:CDS 35.0%
TTCTCCTGTCATATTGGAAT+TGG + tig0028361:9986-10005 Msa1437740:intron 35.0%
AATGCCAATTCCAATATGAC+AGG - tig0028361:9993-10012 None:intergenic 35.0%
CCGGGAAATTCAATATTAGT+AGG + tig0028361:10244-10263 Msa1437740:CDS 35.0%
TGAAATGATCGAGTATGTTC+TGG - tig0028361:10351-10370 None:intergenic 35.0%
GAATTAAAAGGCTGACCTTT+AGG - tig0028361:10404-10423 None:intergenic 35.0%
!! GATCTTTGGAACTTAGTCTT+CGG + tig0028361:10654-10673 Msa1437740:intron 35.0%
TGAAGATCTGAACCAATTTG+GGG + tig0028361:10812-10831 Msa1437740:intron 35.0%
AACCAAACAGCAAGACTAAT+TGG + tig0028361:11026-11045 Msa1437740:intron 35.0%
!! TTCCAATTAGTCTTGCTGTT+TGG - tig0028361:11031-11050 None:intergenic 35.0%
! CTCTATGACACATGATTTTG+GGG - tig0028361:11071-11090 None:intergenic 35.0%
! CACTCTATGACACATGATTT+TGG - tig0028361:11073-11092 None:intergenic 35.0%
ATCATGTGTCATAGAGTGAA+AGG + tig0028361:11075-11094 Msa1437740:intron 35.0%
TCATGTGTCATAGAGTGAAA+GGG + tig0028361:11076-11095 Msa1437740:intron 35.0%
!!! AGATCCACAATGTTGTTTTG+TGG - tig0028361:11332-11351 None:intergenic 35.0%
ACAAAACAACATTGTGGATC+TGG + tig0028361:11331-11350 Msa1437740:intron 35.0%
! TCTGGTTCACATTATAGACA+TGG + tig0028361:11349-11368 Msa1437740:intron 35.0%
!! TGGACATATTAGCTCTGAAA+AGG - tig0028361:11432-11451 None:intergenic 35.0%
CAGAGCTAATATGTCCATAA+TGG + tig0028361:11435-11454 Msa1437740:intron 35.0%
TCTAAGCTGAATCACCATTA+TGG - tig0028361:11452-11471 None:intergenic 35.0%
TGTCTATAGTTTCAGATGTC+TGG - tig0028361:11596-11615 None:intergenic 35.0%
GACAAACCAAAGAATCTATC+AGG + tig0028361:11720-11739 Msa1437740:CDS 35.0%
TATCAGGCTGGAGTTTAAAT+TGG + tig0028361:11736-11755 Msa1437740:CDS 35.0%
! TAATGCTGAGTACTGTTCTG+TGG - tig0028361:7974-7993 None:intergenic 40.0%
ATTAATTTAGGCAGCGTGTC+TGG + tig0028361:7990-8009 Msa1437740:five_prime_UTR 40.0%
! TTCTGTCAGACTGACTTTCA+GGG - tig0028361:8125-8144 None:intergenic 40.0%
!!! TTTTTGGTTTGGGCTTATGC+AGG + tig0028361:8359-8378 Msa1437740:intron 40.0%
AAAAGTCTGTTGGGATTCGA+GGG - tig0028361:8393-8412 None:intergenic 40.0%
GCACATTATAAGTGGTTTCC+TGG - tig0028361:8426-8445 None:intergenic 40.0%
TTCCAAACAGTATCATCAGC+AGG + tig0028361:8523-8542 Msa1437740:intron 40.0%
TCCAAACAGTATCATCAGCA+GGG + tig0028361:8524-8543 Msa1437740:intron 40.0%
TATCATCAGCAGGGTATATG+AGG + tig0028361:8533-8552 Msa1437740:intron 40.0%
ATCATCAGCAGGGTATATGA+GGG + tig0028361:8534-8553 Msa1437740:intron 40.0%
CTTTCTCTATCCAAAAGAGG+TGG - tig0028361:8739-8758 None:intergenic 40.0%
CCTCTTTCTCTATCCAAAAG+AGG - tig0028361:8742-8761 None:intergenic 40.0%
! CCTCTTTTGGATAGAGAAAG+AGG + tig0028361:8739-8758 Msa1437740:intron 40.0%
! CTTTTGGATAGAGAAAGAGG+GGG + tig0028361:8742-8761 Msa1437740:intron 40.0%
TAGACAGAGAACATCAGACA+TGG + tig0028361:8785-8804 Msa1437740:intron 40.0%
AGAGAACATCAGACATGGAT+AGG + tig0028361:8790-8809 Msa1437740:intron 40.0%
GAGAACATCAGACATGGATA+GGG + tig0028361:8791-8810 Msa1437740:intron 40.0%
!! AGCCGCATTTTGATATCAAG+GGG - tig0028361:9070-9089 None:intergenic 40.0%
!! GTAGCCGCATTTTGATATCA+AGG - tig0028361:9072-9091 None:intergenic 40.0%
CAGCCTCTAATCTCAAATTC+TGG + tig0028361:9110-9129 Msa1437740:five_prime_UTR 40.0%
CTCAAGCTTGAAAACTTTCC+AGG + tig0028361:9140-9159 Msa1437740:five_prime_UTR 40.0%
AAGCTTGAAAACTTTCCAGG+TGG + tig0028361:9143-9162 Msa1437740:five_prime_UTR 40.0%
TCAAATGTTTCACATCCACC+TGG - tig0028361:9161-9180 None:intergenic 40.0%
! AAGAAAGCACGACAAATGTG+AGG - tig0028361:9322-9341 None:intergenic 40.0%
CATTTGTCGTGCTTTCTTCA+TGG + tig0028361:9324-9343 Msa1437740:CDS 40.0%
GATGCTGTGATTCTATAGCT+TGG + tig0028361:9420-9439 Msa1437740:CDS 40.0%
CTGTGATTCTATAGCTTGGA+AGG + tig0028361:9424-9443 Msa1437740:CDS 40.0%
CAGCATCTAATCAAGAAAGC+TGG + tig0028361:9477-9496 Msa1437740:CDS 40.0%
CATCTAATCAAGAAAGCTGG+TGG + tig0028361:9480-9499 Msa1437740:CDS 40.0%
GCATAAAATGATCAATGCGG+AGG - tig0028361:9523-9542 None:intergenic 40.0%
TCATGCAATATGCCAAGAGA+TGG + tig0028361:9600-9619 Msa1437740:CDS 40.0%
TCTAACTCCTCATACATTCC+TGG - tig0028361:9629-9648 None:intergenic 40.0%
GGAATGTATGAGGAGTTAGA+AGG + tig0028361:9629-9648 Msa1437740:CDS 40.0%
TTAGAAGGGCTTAGAGGATA+TGG + tig0028361:9644-9663 Msa1437740:CDS 40.0%
TAGAAGGGCTTAGAGGATAT+GGG + tig0028361:9645-9664 Msa1437740:CDS 40.0%
CTTGTTGCAGATGCTAAAGA+TGG + tig0028361:9808-9827 Msa1437740:CDS 40.0%
TCAGACCTTGAAAAGAGAGT+TGG + tig0028361:9863-9882 Msa1437740:CDS 40.0%
! CCATACCAACTCTCTTTTCA+AGG - tig0028361:9871-9890 None:intergenic 40.0%
!! CCTTGAAAAGAGAGTTGGTA+TGG + tig0028361:9868-9887 Msa1437740:CDS 40.0%
! TATGGTGTTAGAAGATGCTG+AGG + tig0028361:9886-9905 Msa1437740:CDS 40.0%
GGAATTGGCATTAATGCTGA+AGG + tig0028361:10001-10020 Msa1437740:intron 40.0%
!! TGGTCATTTTCATAAGCCTC+CGG - tig0028361:10059-10078 None:intergenic 40.0%
! TCTTGCATAGTGCAGTTTTC+GGG - tig0028361:10150-10169 None:intergenic 40.0%
! GTCTTGCATAGTGCAGTTTT+CGG - tig0028361:10151-10170 None:intergenic 40.0%
ATGCAACTGCAACAAAAGTC+CGG + tig0028361:10225-10244 Msa1437740:CDS 40.0%
CATACTCGATCATTTCACGA+TGG + tig0028361:10354-10373 Msa1437740:CDS 40.0%
TAAAAGGCTGACCTTTAGGT+AGG - tig0028361:10400-10419 None:intergenic 40.0%
GACTAAGTTCCAAAGATCCA+GGG - tig0028361:10652-10671 None:intergenic 40.0%
AGACTAAGTTCCAAAGATCC+AGG - tig0028361:10653-10672 None:intergenic 40.0%
GGTATTTAAGCAGATGCTAG+TGG - tig0028361:10769-10788 None:intergenic 40.0%
GAAAACATCTGACCCCAAAT+TGG - tig0028361:10827-10846 None:intergenic 40.0%
! AGTTTTCCATCTGCATGGAA+GGG - tig0028361:10903-10922 None:intergenic 40.0%
! TAGTTTTCCATCTGCATGGA+AGG - tig0028361:10904-10923 None:intergenic 40.0%
!!! TCACTAGTTTTCCATCTGCA+TGG - tig0028361:10908-10927 None:intergenic 40.0%
!! ATGACACATGATTTTGGGGA+GGG - tig0028361:11067-11086 None:intergenic 40.0%
!! TATGACACATGATTTTGGGG+AGG - tig0028361:11068-11087 None:intergenic 40.0%
! ACATGGCATCTAAGATAGTG+TGG + tig0028361:11366-11385 Msa1437740:intron 40.0%
! TGCGTTGTATACTTTGGTTC+AGG - tig0028361:11483-11502 None:intergenic 40.0%
TGAACCAAAGTATACAACGC+AGG + tig0028361:11482-11501 Msa1437740:intron 40.0%
AGAACCTGCGTTGTATACTT+TGG - tig0028361:11489-11508 None:intergenic 40.0%
AAGTGGAATCAACTCTCAAC+AGG + tig0028361:11563-11582 Msa1437740:CDS 40.0%
CGAGAATGTAAAGTCCAACA+TGG + tig0028361:11638-11657 Msa1437740:CDS 40.0%
AACCAAAGAATCTATCAGGC+TGG + tig0028361:11724-11743 Msa1437740:CDS 40.0%
TCCTTCCATGTAAAACCAAC+AGG + tig0028361:11774-11793 Msa1437740:CDS 40.0%
!!! TCCTGTTGGTTTTACATGGA+AGG - tig0028361:11778-11797 None:intergenic 40.0%
TTCCATGTAAAACCAACAGG+AGG + tig0028361:11777-11796 Msa1437740:CDS 40.0%
GAGCTAATTGCTGCAAGCTT+GGG - tig0028361:8060-8079 None:intergenic 45.0%
TGAGCTAATTGCTGCAAGCT+TGG - tig0028361:8061-8080 None:intergenic 45.0%
! ACTTTCAGGGTCTTGTGCTT+GGG - tig0028361:8112-8131 None:intergenic 45.0%
! GTTCTGTCAGACTGACTTTC+AGG - tig0028361:8126-8145 None:intergenic 45.0%
AAAGTCTGTTGGGATTCGAG+GGG - tig0028361:8392-8411 None:intergenic 45.0%
GAAAAGTCTGTTGGGATTCG+AGG - tig0028361:8394-8413 None:intergenic 45.0%
ACCCTGCTGATGATACTGTT+TGG - tig0028361:8528-8547 None:intergenic 45.0%
CACACACACACAAACACACT+AGG - tig0028361:8679-8698 None:intergenic 45.0%
TCTATCCAAAAGAGGTGGTG+GGG - tig0028361:8734-8753 None:intergenic 45.0%
CTCTATCCAAAAGAGGTGGT+GGG - tig0028361:8735-8754 None:intergenic 45.0%
TCTCTATCCAAAAGAGGTGG+TGG - tig0028361:8736-8755 None:intergenic 45.0%
GGGAAAATTTCCCTCAAGAG+AGG + tig0028361:8762-8781 Msa1437740:intron 45.0%
TCTCTGTCTACCTCTCTTGA+GGG - tig0028361:8775-8794 None:intergenic 45.0%
TTCTCTGTCTACCTCTCTTG+AGG - tig0028361:8776-8795 None:intergenic 45.0%
CAAAATGCGGCTACATAGGA+CGG + tig0028361:9078-9097 Msa1437740:five_prime_UTR 45.0%
CCTCCCTATAGACTTCAACT+CGG + tig0028361:9205-9224 Msa1437740:five_prime_UTR 45.0%
TGTGAGGAAAGCTTCAGTCT+TGG - tig0028361:9306-9325 None:intergenic 45.0%
TAAAAATGAGCCCGCAAGTG+AGG + tig0028361:9451-9470 Msa1437740:CDS 45.0%
AAGCTGGTGGTTCGATGATT+TGG + tig0028361:9493-9512 Msa1437740:CDS 45.0%
AAAATGATCAATGCGGAGGG+TGG - tig0028361:9519-9538 None:intergenic 45.0%
GGAACCAAACCAGAGATCAT+TGG + tig0028361:9569-9588 Msa1437740:CDS 45.0%
ATGGCTACCAGGAATGTATG+AGG + tig0028361:9619-9638 Msa1437740:CDS 45.0%
!! AGAGAGCACTAGACATAGCA+AGG + tig0028361:9715-9734 Msa1437740:CDS 45.0%
!!! GTGCAGTTTTCGGGTGAATT+AGG - tig0028361:10141-10160 None:intergenic 45.0%
TGCAACTGCAACAAAAGTCC+GGG + tig0028361:10226-10245 Msa1437740:CDS 45.0%
GATCTGTTGCAACTTCAGCT+AGG - tig0028361:10284-10303 None:intergenic 45.0%
AGCCATTGACACCTACCTAA+AGG + tig0028361:10386-10405 Msa1437740:CDS 45.0%
GACCTTTAGGTAGGTGTCAA+TGG - tig0028361:10391-10410 None:intergenic 45.0%
TTGGTCAGTTAGGGAAGGAT+GGG - tig0028361:10516-10535 None:intergenic 45.0%
TCTACGGTCTTGGTCAGTTA+GGG - tig0028361:10525-10544 None:intergenic 45.0%
TTCTACGGTCTTGGTCAGTT+AGG - tig0028361:10526-10545 None:intergenic 45.0%
ACCTGGACAACTGTTCTTAG+AGG - tig0028361:10623-10642 None:intergenic 45.0%
AAGATCCAGGGAACTTTACC+TGG - tig0028361:10640-10659 None:intergenic 45.0%
GGTAAAGTTCCCTGGATCTT+TGG + tig0028361:10640-10659 Msa1437740:intron 45.0%
AGAAATCCCTTCCATGCAGA+TGG + tig0028361:10894-10913 Msa1437740:intron 45.0%
AGCAATGCAAGAGAAGGAAC+CGG + tig0028361:11536-11555 Msa1437740:CDS 45.0%
! GAGTTGATTCCACTTTGTGC+CGG - tig0028361:11558-11577 None:intergenic 45.0%
GTTCTGCAGTTCTACCATGT+TGG - tig0028361:11655-11674 None:intergenic 45.0%
CTCCAGCCTGATAGATTCTT+TGG - tig0028361:11729-11748 None:intergenic 45.0%
CAGGCTGGAGTTTAAATTGG+CGG + tig0028361:11739-11758 Msa1437740:CDS 45.0%
!!! CACCTCCTGTTGGTTTTACA+TGG - tig0028361:11782-11801 None:intergenic 45.0%
! TCTCTAGTTTCACCTCCTGT+TGG - tig0028361:11792-11811 None:intergenic 45.0%
ACAGGAGGTGAAACTAGAGA+TGG + tig0028361:11792-11811 Msa1437740:CDS 45.0%
!! TTAGATATAATATAAAATTT+AGG - tig0028361:11950-11969 None:intergenic 5.0%
! GACTTTCAGGGTCTTGTGCT+TGG - tig0028361:8113-8132 None:intergenic 50.0%
! CCGAGTTGAAGTCTATAGGG+AGG - tig0028361:9208-9227 None:intergenic 50.0%
AGATGCTGCATCCTCACTTG+CGG - tig0028361:9465-9484 None:intergenic 50.0%
TATGCCAAGAGATGGCTACC+AGG + tig0028361:9608-9627 Msa1437740:CDS 50.0%
CATTCCTGGTAGCCATCTCT+TGG - tig0028361:9615-9634 None:intergenic 50.0%
GAGGAGTTAGAAGGGCTTAG+AGG + tig0028361:9638-9657 Msa1437740:CDS 50.0%
CATGGGACAGCATCTTGTTG+AGG - tig0028361:9782-9801 None:intergenic 50.0%
CTTGGTCAGTTAGGGAAGGA+TGG - tig0028361:10517-10536 None:intergenic 50.0%
TGCAGAGTCTTCTACGGTCT+TGG - tig0028361:10535-10554 None:intergenic 50.0%
! GCCTCTAAGAACAGTTGTCC+AGG + tig0028361:10619-10638 Msa1437740:CDS 50.0%
GTTGTCCAGGTAAAGTTCCC+TGG + tig0028361:10632-10651 Msa1437740:intron 50.0%
GAGAGCAGCAATGCAAGAGA+AGG + tig0028361:11530-11549 Msa1437740:CDS 50.0%
!! CGAGTGTGGTTTGGTGATGT+GGG - tig0028361:11828-11847 None:intergenic 50.0%
!! ACGAGTGTGGTTTGGTGATG+TGG - tig0028361:11829-11848 None:intergenic 50.0%
GATCTACGACGAGTGTGGTT+TGG - tig0028361:11837-11856 None:intergenic 50.0%
!! TCCAAAAGAGGTGGTGGGGT+GGG - tig0028361:8730-8749 None:intergenic 55.0%
!! ATCCAAAAGAGGTGGTGGGG+TGG - tig0028361:8731-8750 None:intergenic 55.0%
ATGCGGCTACATAGGACGGT+CGG + tig0028361:9082-9101 Msa1437740:five_prime_UTR 55.0%
GATGCTGCATCCTCACTTGC+GGG - tig0028361:9464-9483 None:intergenic 55.0%
GGGCTTAGAGGATATGGGCA+TGG + tig0028361:9650-9669 Msa1437740:CDS 55.0%
GGGACAGCATCTTGTTGAGG+AGG - tig0028361:9779-9798 None:intergenic 55.0%
CGGTCTTGGTCAGTTAGGGA+AGG - tig0028361:10521-10540 None:intergenic 55.0%
GAGAAGGAACCGGCACAAAG+TGG + tig0028361:11546-11565 Msa1437740:CDS 55.0%
GTAGATCAGCCCCGAGAAGA+AGG + tig0028361:11850-11869 Msa1437740:CDS 55.0%
ACACGGAGAGCCTTCTTCTC+GGG - tig0028361:11863-11882 None:intergenic 55.0%
GACACGGAGAGCCTTCTTCT+CGG - tig0028361:11864-11883 None:intergenic 55.0%
! TCCCACCCCACCACCTCTTT+TGG + tig0028361:8726-8745 Msa1437740:intron 60.0%
GGGCTGATCTACGACGAGTG+TGG - tig0028361:11842-11861 None:intergenic 60.0%
CACGGAGAGCCTTCTTCTCG+GGG - tig0028361:11862-11881 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0028361 gene 7929 11980 7929 ID=Msa1437740;Name=Msa1437740
tig0028361 mRNA 7929 11980 7929 ID=Msa1437740-mRNA-1;Parent=Msa1437740;Name=Msa1437740-mRNA-1;_AED=0.07;_eAED=0.07;_QI=571|1|1|1|0.8|0.83|6|93|493
tig0028361 exon 7929 8225 7929 ID=Msa1437740-mRNA-1:exon:10868;Parent=Msa1437740-mRNA-1
tig0028361 exon 8381 8450 8381 ID=Msa1437740-mRNA-1:exon:10869;Parent=Msa1437740-mRNA-1
tig0028361 exon 9065 9963 9065 ID=Msa1437740-mRNA-1:exon:10870;Parent=Msa1437740-mRNA-1
tig0028361 exon 10134 10407 10134 ID=Msa1437740-mRNA-1:exon:10871;Parent=Msa1437740-mRNA-1
tig0028361 exon 10512 10640 10512 ID=Msa1437740-mRNA-1:exon:10872;Parent=Msa1437740-mRNA-1
tig0028361 exon 11504 11980 11504 ID=Msa1437740-mRNA-1:exon:10873;Parent=Msa1437740-mRNA-1
tig0028361 five_prime_UTR 7929 8225 7929 ID=Msa1437740-mRNA-1:five_prime_utr;Parent=Msa1437740-mRNA-1
tig0028361 five_prime_UTR 8381 8450 8381 ID=Msa1437740-mRNA-1:five_prime_utr;Parent=Msa1437740-mRNA-1
tig0028361 five_prime_UTR 9065 9268 9065 ID=Msa1437740-mRNA-1:five_prime_utr;Parent=Msa1437740-mRNA-1
tig0028361 CDS 9269 9963 9269 ID=Msa1437740-mRNA-1:cds;Parent=Msa1437740-mRNA-1
tig0028361 CDS 10134 10407 10134 ID=Msa1437740-mRNA-1:cds;Parent=Msa1437740-mRNA-1
tig0028361 CDS 10512 10640 10512 ID=Msa1437740-mRNA-1:cds;Parent=Msa1437740-mRNA-1
tig0028361 CDS 11504 11887 11504 ID=Msa1437740-mRNA-1:cds;Parent=Msa1437740-mRNA-1
tig0028361 three_prime_UTR 11888 11980 11888 ID=Msa1437740-mRNA-1:three_prime_utr;Parent=Msa1437740-mRNA-1
Gene Sequence

>Msa1437740

ATGACTGAAGAACTAGAAGATGGCAATCTCATTTCCAAGACTGAAGCTTTCCTCACATTTGTCGTGCTTTCTTCATGGAAAGACACTGTTACTGTTCTGAAATCTTGTGAAAATCTATCTCCATGGGCTGAAAATATCCAAATTGTTAGAAGATGCTGTGATTCTATAGCTTGGAAGGCTACTAAAAATGAGCCCGCAAGTGAGGATGCAGCATCTAATCAAGAAAGCTGGTGGTTCGATGATTTGGCCACCCTCCGCATTGATCATTTTATGCGGATAATTTCAGCAATTAGGGCAAAGGGAACCAAACCAGAGATCATTGGTAAATGTATCATGCAATATGCCAAGAGATGGCTACCAGGAATGTATGAGGAGTTAGAAGGGCTTAGAGGATATGGGCATGGAAAAAGTAATCTACAGTTCACTATATTTAGCATGAAGAAAGAAGAGAGCACTAGACATAGCAAGGAGCAAACGACTATTATTGAAAGCCTAATAAGCATAATTCCTCCTCAACAAGATGCTGTCCCATGTAAGTTCTTGTTGCAGATGCTAAAGATGGCAATGATGTATTCTGTATCATCAGCTCTCATTTCAGACCTTGAAAAGAGAGTTGGTATGGTGTTAGAAGATGCTGAGGTGAATGATCTCTTAATTCCTAGATATCAAAATGGAGGTCAAGGAAAAATGGTCAACATGCCTAATTCACCCGAAAACTGCACTATGCAAGACATAGATGTAGTACAACGGATTGTTGAATACTTCTTGATGCATGAAAAACAGCAAATGCAACTGCAACAAAAGTCCGGGAAATTCAATATTAGTAGGCTTTTGGACAATTACCTAGCTGAAGTTGCAACAGATCCAAATCTTTCAATTACAATGTTTCAAGTCTTAGCTGAATTACTGCCAGAACATACTCGATCATTTCACGATGGTCTATATAGAGCCATTGACACCTACCTAAAGACCCATCCTTCCCTAACTGACCAAGACCGTAGAAGACTCTGCAAAATAATGAACTGTGAAAAACTCTCACTTGATGCATGCATACATGCTGCACAAAATGATAGATTGCCTCTAAGAACAGTTGTCCAGGTTCTATTTGCAGAGCAAGTAAAGATGAGAGCAGCAATGCAAGAGAAGGAACCGGCACAAAGTGGAATCAACTCTCAACAGGATGTAAACCAGACATCTGAAACTATAGACATAAAAACACTCAAAGCAGAACTCGAGAATGTAAAGTCCAACATGGTAGAACTGCAGAACGACTACTTTGAGTTGCAAGAAGAGTATAAAAAGTTAACTAGCAACGACAAACCAAAGAATCTATCAGGCTGGAGTTTAAATTGGCGGAAGATCAAGAAATCCTTCCATGTAAAACCAACAGGAGGTGAAACTAGAGATGGACAAGATATGCCCACATCACCAAACCACACTCGTCGTAGATCAGCCCCGAGAAGAAGGCTCTCCGTGTCTTAA
Protein sequence

>Msa1437740

MTEELEDGNLISKTEAFLTFVVLSSWKDTVTVLKSCENLSPWAENIQIVRRCCDSIAWKATKNEPASEDAASNQESWWFDDLATLRIDHFMRIISAIRAKGTKPEIIGKCIMQYAKRWLPGMYEELEGLRGYGHGKSNLQFTIFSMKKEESTRHSKEQTTIIESLISIIPPQQDAVPCKFLLQMLKMAMMYSVSSALISDLEKRVGMVLEDAEVNDLLIPRYQNGGQGKMVNMPNSPENCTMQDIDVVQRIVEYFLMHEKQQMQLQQKSGKFNISRLLDNYLAEVATDPNLSITMFQVLAELLPEHTRSFHDGLYRAIDTYLKTHPSLTDQDRRRLCKIMNCEKLSLDACIHAAQNDRLPLRTVVQVLFAEQVKMRAAMQEKEPAQSGINSQQDVNQTSETIDIKTLKAELENVKSNMVELQNDYFELQEEYKKLTSNDKPKNLSGWSLNWRKIKKSFHVKPTGGETRDGQDMPTSPNHTRRRSAPRRRLSVS*