Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0007930 | sp|Q8VYD6|PP374_ARATH | 59.325 | 563 | 224 | 1 | 16 | 578 | 17 | 574 | 0.0 | 687 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001420 | Msa0007930 | 0.828895 | 6.971515e-55 | -8.615850e-47 |
| Msa0001540 | Msa0007930 | 0.847538 | 1.085521e-59 | -8.615850e-47 |
| Msa0007930 | Msa0009210 | 0.825931 | 3.580341e-54 | -8.615850e-47 |
| Msa0007930 | Msa0025830 | 0.810153 | 1.327978e-50 | -8.615850e-47 |
| Msa0007930 | Msa0034320 | 0.813019 | 3.162756e-51 | -8.615850e-47 |
| Msa0007930 | Msa0037300 | 0.801920 | 7.170193e-49 | -8.615850e-47 |
| Msa0007930 | Msa0045530 | 0.807107 | 5.941633e-50 | -8.615850e-47 |
| Msa0007930 | Msa0046800 | 0.811259 | 7.657777e-51 | -8.615850e-47 |
| Msa0007930 | Msa0049990 | 0.832465 | 9.306213e-56 | -8.615850e-47 |
| Msa0007930 | Msa0056370 | 0.885055 | 1.130782e-71 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0007930 | MtrunA17_Chr1g0147931 | 95.439 | 592 | 20 | 2 | 1 | 592 | 1 | 585 | 0.0 | 1117 |
| Msa0007930 | MtrunA17_Chr8g0392911 | 25.234 | 321 | 215 | 8 | 56 | 374 | 170 | 467 | 2.60e-20 | 95.1 |
| Msa0007930 | MtrunA17_Chr8g0392911 | 24.118 | 340 | 213 | 11 | 59 | 396 | 313 | 609 | 7.25e-17 | 84.3 |
| Msa0007930 | MtrunA17_Chr8g0392911 | 23.580 | 352 | 239 | 10 | 80 | 429 | 124 | 447 | 1.74e-12 | 70.5 |
| Msa0007930 | MtrunA17_Chr3g0136851 | 24.352 | 386 | 258 | 9 | 27 | 408 | 216 | 571 | 3.18e-20 | 95.5 |
| Msa0007930 | MtrunA17_Chr3g0136851 | 25.085 | 295 | 200 | 6 | 80 | 374 | 830 | 1103 | 1.40e-18 | 90.1 |
| Msa0007930 | MtrunA17_Chr3g0136851 | 23.058 | 412 | 245 | 15 | 48 | 416 | 336 | 718 | 1.12e-16 | 84.3 |
| Msa0007930 | MtrunA17_Chr3g0136851 | 20.917 | 349 | 220 | 9 | 67 | 376 | 634 | 965 | 1.22e-11 | 68.2 |
| Msa0007930 | MtrunA17_Chr3g0133151 | 26.275 | 255 | 172 | 5 | 78 | 331 | 196 | 435 | 2.53e-19 | 91.7 |
| Msa0007930 | MtrunA17_Chr3g0133151 | 25.075 | 335 | 193 | 11 | 68 | 364 | 44 | 358 | 3.51e-13 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0007930 | AT5G10690.1 | 59.325 | 563 | 224 | 1 | 16 | 578 | 17 | 574 | 0.0 | 687 |
| Msa0007930 | AT5G10690.2 | 59.325 | 563 | 224 | 1 | 16 | 578 | 17 | 574 | 0.0 | 687 |
| Msa0007930 | AT1G64580.1 | 25.688 | 327 | 197 | 8 | 80 | 376 | 183 | 493 | 9.43e-23 | 102 |
| Msa0007930 | AT1G09900.1 | 25.210 | 357 | 236 | 11 | 19 | 371 | 180 | 509 | 1.21e-22 | 102 |
| Msa0007930 | AT1G09900.1 | 25.098 | 255 | 169 | 8 | 82 | 333 | 345 | 580 | 3.20e-11 | 66.6 |
| Msa0007930 | AT1G12775.1 | 28.517 | 263 | 172 | 6 | 73 | 335 | 255 | 501 | 5.79e-22 | 100 |
| Msa0007930 | AT1G09680.1 | 27.221 | 349 | 214 | 10 | 79 | 411 | 238 | 562 | 6.61e-22 | 100 |
| Msa0007930 | AT1G62914.1 | 26.885 | 305 | 193 | 9 | 64 | 365 | 239 | 516 | 5.87e-21 | 97.1 |
| Msa0007930 | AT1G63400.1 | 26.667 | 345 | 211 | 12 | 64 | 393 | 246 | 563 | 6.73e-21 | 97.1 |
| Msa0007930 | AT1G62670.2 | 27.507 | 349 | 207 | 12 | 64 | 395 | 242 | 561 | 7.59e-21 | 97.1 |
Find 142 sgRNAs with CRISPR-Local
Find 361 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATAGGTGGGCCCACCAC+TGG | 0.426540 | 1_1:-11348490 | Msa0007930:three_prime_UTR |
| AAACCCTCAACCGGTTCAGA+AGG | 0.579685 | 1_1:+11349417 | None:intergenic |
| AAAGCAACACGGCCACTACA+AGG | 0.595532 | 1_1:-11349381 | Msa0007930:CDS |
| AAATACAGAAACAGGTTGGT+TGG | 0.498251 | 1_1:-11350563 | Msa0007930:CDS |
| AAATAGGTGGGCCCACCACT+GGG | 0.566651 | 1_1:-11348489 | Msa0007930:three_prime_UTR |
| AAGAGAGAGTTAGTGAAGAG+AGG | 0.533305 | 1_1:-11348575 | Msa0007930:three_prime_UTR |
| AAGATTGTTCCTGAAGTATC+TGG | 0.404575 | 1_1:+11350435 | None:intergenic |
| AAGGTGGTCAGCTTCTTCTT+GGG | 0.465281 | 1_1:+11350404 | None:intergenic |
| AATGATGGAGAGGGTTTGAG+TGG | 0.462781 | 1_1:+11354128 | None:intergenic |
| AATGGCCTCCTTGCCTGTTA+TGG | 0.294395 | 1_1:-11352221 | Msa0007930:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTTCAATTTCATTCAGAATC+TGG | + | chr1_1:11348710-11348729 | None:intergenic | 30.0% | |
| TTGTGAGTATGACAATGTAT+AGG | - | chr1_1:11349136-11349155 | Msa0007930:intron | 30.0% | |
| TGGCTAAAAACCAATTGTAA+AGG | - | chr1_1:11349363-11349382 | Msa0007930:CDS | 30.0% | |
| GGCTAAAAACCAATTGTAAA+GGG | - | chr1_1:11349364-11349383 | Msa0007930:CDS | 30.0% | |
| ATTATTATGGTATGTCTGGA+TGG | + | chr1_1:11349828-11349847 | None:intergenic | 30.0% | |
| ATTTATAAAGGGTTTAGGCA+AGG | - | chr1_1:11349937-11349956 | Msa0007930:intron | 30.0% | |
| TTTAGTAAACCAAAGACCAA+TGG | + | chr1_1:11350043-11350062 | None:intergenic | 30.0% | |
| AAAGTAATGTACACTTAGCA+AGG | + | chr1_1:11350188-11350207 | None:intergenic | 30.0% | |
| CATTAAAAAGTCATGTTGAC+TGG | - | chr1_1:11350388-11350407 | Msa0007930:CDS | 30.0% | |
| CATATTTCAGTAGTAGAGAT+TGG | - | chr1_1:11350581-11350600 | Msa0007930:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 11348469 | 11354232 | 11348469 | ID=Msa0007930;Name=Msa0007930 |
| chr1_1 | mRNA | 11348469 | 11354232 | 11348469 | ID=Msa0007930-mRNA-1;Parent=Msa0007930;Name=Msa0007930-mRNA-1;_AED=0.15;_eAED=0.15;_QI=50|1|1|1|1|1|11|237|593 |
| chr1_1 | exon | 11348469 | 11348957 | 11348469 | ID=Msa0007930-mRNA-1:exon:4228;Parent=Msa0007930-mRNA-1 |
| chr1_1 | exon | 11349269 | 11349445 | 11349269 | ID=Msa0007930-mRNA-1:exon:4227;Parent=Msa0007930-mRNA-1 |
| chr1_1 | exon | 11349731 | 11349799 | 11349731 | ID=Msa0007930-mRNA-1:exon:4226;Parent=Msa0007930-mRNA-1 |
| chr1_1 | exon | 11350207 | 11350284 | 11350207 | ID=Msa0007930-mRNA-1:exon:4225;Parent=Msa0007930-mRNA-1 |
| chr1_1 | exon | 11350374 | 11350606 | 11350374 | ID=Msa0007930-mRNA-1:exon:4224;Parent=Msa0007930-mRNA-1 |
| chr1_1 | exon | 11351266 | 11351401 | 11351266 | ID=Msa0007930-mRNA-1:exon:4223;Parent=Msa0007930-mRNA-1 |
| chr1_1 | exon | 11351722 | 11351790 | 11351722 | ID=Msa0007930-mRNA-1:exon:4222;Parent=Msa0007930-mRNA-1 |
| chr1_1 | exon | 11351877 | 11352038 | 11351877 | ID=Msa0007930-mRNA-1:exon:4221;Parent=Msa0007930-mRNA-1 |
| chr1_1 | exon | 11352163 | 11352260 | 11352163 | ID=Msa0007930-mRNA-1:exon:4220;Parent=Msa0007930-mRNA-1 |
| chr1_1 | exon | 11352622 | 11352754 | 11352622 | ID=Msa0007930-mRNA-1:exon:4219;Parent=Msa0007930-mRNA-1 |
| chr1_1 | exon | 11353808 | 11354232 | 11353808 | ID=Msa0007930-mRNA-1:exon:4218;Parent=Msa0007930-mRNA-1 |
| chr1_1 | five_prime_UTR | 11354183 | 11354232 | 11354183 | ID=Msa0007930-mRNA-1:five_prime_utr;Parent=Msa0007930-mRNA-1 |
| chr1_1 | CDS | 11353808 | 11354182 | 11353808 | ID=Msa0007930-mRNA-1:cds;Parent=Msa0007930-mRNA-1 |
| chr1_1 | CDS | 11352622 | 11352754 | 11352622 | ID=Msa0007930-mRNA-1:cds;Parent=Msa0007930-mRNA-1 |
| chr1_1 | CDS | 11352163 | 11352260 | 11352163 | ID=Msa0007930-mRNA-1:cds;Parent=Msa0007930-mRNA-1 |
| chr1_1 | CDS | 11351877 | 11352038 | 11351877 | ID=Msa0007930-mRNA-1:cds;Parent=Msa0007930-mRNA-1 |
| chr1_1 | CDS | 11351722 | 11351790 | 11351722 | ID=Msa0007930-mRNA-1:cds;Parent=Msa0007930-mRNA-1 |
| chr1_1 | CDS | 11351266 | 11351401 | 11351266 | ID=Msa0007930-mRNA-1:cds;Parent=Msa0007930-mRNA-1 |
| chr1_1 | CDS | 11350374 | 11350606 | 11350374 | ID=Msa0007930-mRNA-1:cds;Parent=Msa0007930-mRNA-1 |
| chr1_1 | CDS | 11350207 | 11350284 | 11350207 | ID=Msa0007930-mRNA-1:cds;Parent=Msa0007930-mRNA-1 |
| chr1_1 | CDS | 11349731 | 11349799 | 11349731 | ID=Msa0007930-mRNA-1:cds;Parent=Msa0007930-mRNA-1 |
| chr1_1 | CDS | 11349269 | 11349445 | 11349269 | ID=Msa0007930-mRNA-1:cds;Parent=Msa0007930-mRNA-1 |
| chr1_1 | CDS | 11348706 | 11348957 | 11348706 | ID=Msa0007930-mRNA-1:cds;Parent=Msa0007930-mRNA-1 |
| chr1_1 | three_prime_UTR | 11348469 | 11348705 | 11348469 | ID=Msa0007930-mRNA-1:three_prime_utr;Parent=Msa0007930-mRNA-1 |
| Gene Sequence |
>Msa0007930 ATGCTTCTAACTCACGCTGCTTTCTCTTCAATCCACTCAAACCCTCTCCATCATTCTTCATACATTCCCATTTCTTCGTGTTCTTCATCTTCTCCGATACGACGTCGTTTATTTACCACTCCTCGCAAAACGAATCTGAAACACCTCACATCCCGCATAGTTCAACTCACTCGCCGAAAACAGCTTCACCAGATTCTGAATGAAATTGAAGTCGCGAAGAAACGTTTTGGAAAATTGAATACGATTGTGATGAATGCGGTTATGGAAGCTTGTGTTCGTTGCGGTGATATCGATTCAGCTCTTAACATTTTTGATGAGATGAAGAATCCTGAAGCTTGTGGTGTTGATACTGTCACCTATGCCACGCTTTTGAAGGGTTTAGGCAAGGCGCATAGATTTGATGAAGCTTTTCGATTGCTTGAATCTGTCGAAAATGGTACTGCCGTGGGAAGTCCAAAGTTGTCAGCACCATTGGTCTTTGGTTTACTAAATGCCTTAGCAGAAGCAGGAGACCTGCGTCGTGCAAATGGCCTCCTTGCCTGTTATGGTTCCGTGCTTCGTGAAGGCGAACATTTTTCAGTCTCAGTATACAACCTTCTAATAAAGGGGCATATAAACTCAGGCTGTCCTCATACTGCAATAAACATGCTCAATGAAATTTTACATCAAGGAATAATGCCTGACAGGCTCACATATAACACACTAATTTTAGCATGTGTTCAGAGTGAAAAGTTGGATGTTGCCATGCAATTATTTGGGGAAATGAAGGAAAAAGCGCAGGAATTCATCCATGATGATCTGTTTCCAGATATCGTTACATACACAACATTGCTCAAGGGTTTGGGGCAGGCAAGGGATCTCGTTTCAGTTATGCAAATAGTGCTGGAGATGAAATCATGTTCTGAGTTATACATTGATAGAACTGCTTACACAGCAATAGTTGATGCTTTACTAAAATGTGGCTCAGTTAAAGGTGCTCTGTGTATTTTTGGAGAAATACAGAAACAGGTTGGTTGGAATCCAGAGTTGAGACCGAAGCCTCATCTCTATCTTTCCATGATGCGTGCCTTTGCTTCTAGAGGAGATTATGATCTGGTTAAAAACTTGCATAAGCGGATTTTGCCAGATACTTCAGGAACAATCTTACCAGTTTCCCAAGAAGAAGCTGACCACCTTCTAATGGAAGCAGCCCTGAACGCTGGCCAGGTTGATGTCGCTACTAAAACTCTTATGTATATTGTTTCAAGATGGAAGGGCATATCTTGGACGAGTCATGGAGGCATGGTTGCTTCTCGTATTGAGGCCTTATTGGGATTCTCAGCATCTGTATTCAATCCTTTCTTACTTCCTCAGGTTTCACCTTCTGAACCGGTTGAGGGTTTCATGATGTCATTTAAAGCAACACGGCCACTACAAGGTACCATAAAGCTGAAGGATGTGGTGTCGCGTTTCTTTAATGATGCAGTAGTTCCTATTGTAGATGAGTGGGGCAGTTGTACAGGGCTGTTACACCGTGAAGACTGCAATCAGTTGGATGCTCCACTTTCAACAATGATGAGAAGTCCTCCTCCCAGTGTTACAGCTTCAACATCTGTTGGTCATGTAATTGATTTAATTTTGGAGAAGGGTTACCTAATGGTTATCATTGTAAATTGCACTGACTCGTATGCTACTTCATACGGCTGTAGAGCAGTTGGAGTTTTTACATTAGAACAATTGAATAGGCTCATCAAACCTGCACCTGAAGTGAAAGAGACAGACCTTTCACTCTGCAGACCATAG |
| Protein sequence |
>Msa0007930 MLLTHAAFSSIHSNPLHHSSYIPISSCSSSSPIRRRLFTTPRKTNLKHLTSRIVQLTRRKQLHQILNEIEVAKKRFGKLNTIVMNAVMEACVRCGDIDSALNIFDEMKNPEACGVDTVTYATLLKGLGKAHRFDEAFRLLESVENGTAVGSPKLSAPLVFGLLNALAEAGDLRRANGLLACYGSVLREGEHFSVSVYNLLIKGHINSGCPHTAINMLNEILHQGIMPDRLTYNTLILACVQSEKLDVAMQLFGEMKEKAQEFIHDDLFPDIVTYTTLLKGLGQARDLVSVMQIVLEMKSCSELYIDRTAYTAIVDALLKCGSVKGALCIFGEIQKQVGWNPELRPKPHLYLSMMRAFASRGDYDLVKNLHKRILPDTSGTILPVSQEEADHLLMEAALNAGQVDVATKTLMYIVSRWKGISWTSHGGMVASRIEALLGFSASVFNPFLLPQVSPSEPVEGFMMSFKATRPLQGTIKLKDVVSRFFNDAVVPIVDEWGSCTGLLHREDCNQLDAPLSTMMRSPPPSVTASTSVGHVIDLILEKGYLMVIIVNCTDSYATSYGCRAVGVFTLEQLNRLIKPAPEVKETDLSLCRP* |
