AlfalfaGEDB | Msa1411690

M. sativa cultivar zhongmu-4 / Msa1411690

KEGG: K02356
GO: -
PFAM: PF08207.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1411690	sp\|B1XKV1\|EFP_SYNP2	62.500	184	68	1	44	227	2	184	8.88e-81	242

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1411690	A0A1S2XPN6	86.695	233	26	2	1	228	1	233	2.37e-143	409

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa1411690	0.813209	2.873355e-51	-8.615850e-47
Msa0001420	Msa1411690	0.841223	5.413594e-58	-8.615850e-47
Msa0001510	Msa1411690	0.886247	4.037470e-72	-8.615850e-47
Msa0001540	Msa1411690	0.900534	6.651960e-78	-8.615850e-47
Msa0002060	Msa1411690	0.842238	2.921144e-58	-8.615850e-47
Msa0002690	Msa1411690	0.818551	1.847583e-52	-8.615850e-47
Msa0002700	Msa1411690	0.812848	3.447624e-51	-8.615850e-47
Msa0004180	Msa1411690	0.838945	2.126541e-57	-8.615850e-47
Msa0007930	Msa1411690	0.807890	4.052903e-50	-8.615850e-47
Msa0009210	Msa1411690	0.846650	1.900439e-59	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1411690	MtrunA17_Chr7g0244891	90.129	233	18	1	1	228	1	233	7.40e-139	388
Msa1411690	MtrunA17_Chr3g0121231	26.984	189	124	4	44	228	62	240	1.08e-12	65.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1411690	AT3G08740.1	68.559	229	69	3	2	228	9	236	2.69e-111	319
Msa1411690	AT4G26310.1	27.941	204	136	5	29	227	58	255	1.35e-19	84.7
Msa1411690	AT4G26310.2	27.624	181	120	5	29	204	58	232	1.44e-13	68.6

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Find 67 sgRNAs with CRISPR-Local

Find 351 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAAGTGGCTGAAAGA+AGG	0.539823	tig0023826:+47845	Msa1411690:CDS
AAACAGTTCACATATAAAGA+TGG	0.561471	tig0023826:+47539	Msa1411690:CDS
AAAGTGGCTGAAAGAAGGAA+TGG	0.581341	tig0023826:+47850	Msa1411690:CDS
AAATATAGGCGAGGAAATAC+TGG	0.432665	tig0023826:+49237	Msa1411690:CDS
AAGCCAGCAACTCTCGACAC+TGG	0.613570	tig0023826:+49187	Msa1411690:CDS
AAGCTTAAGAATCATCTTAC+TGG	0.330470	tig0023826:+44939	Msa1411690:CDS
AAGGTAGTCGATGTGGATCC+GGG	0.490283	tig0023826:+48623	Msa1411690:CDS
AAGTTGGGAACAAAACAAAG+TGG	0.698694	tig0023826:+47834	Msa1411690:CDS
ACCTTAAGCTTGACTGTTAT+AGG	0.325039	tig0023826:-48605	None:intergenic
ACGACAACACAACATGATAG+CGG	0.677593	tig0023826:+44525	Msa1411690:exon

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTAGTGAAAAGATGAAAGTG+GGG	-	tig0023826:44664-44683	None:intergenic	30.0%
GTTAGTGAAAAGATGAAAGT+GGG	-	tig0023826:44665-44684	None:intergenic	30.0%
GAGATTAGTGGAATTAAGAA+GGG	-	tig0023826:44702-44721	None:intergenic	30.0%
AGAGATTAGTGGAATTAAGA+AGG	-	tig0023826:44703-44722	None:intergenic	30.0%
GAAAACAACAAAGAGATTAG+TGG	-	tig0023826:44714-44733	None:intergenic	30.0%
AAGCTTAAGAATCATCTTAC+TGG	+	tig0023826:44939-44958	Msa1411690:CDS	30.0%
GAATGTTAAGAATCTCACAT+CGG	+	tig0023826:45068-45087	Msa1411690:intron	30.0%
AGTCTGACAATGTTTATAAG+TGG	+	tig0023826:45100-45119	Msa1411690:intron	30.0%
GTCTGACAATGTTTATAAGT+GGG	+	tig0023826:45101-45120	Msa1411690:intron	30.0%
GAAAAAACATGATTGAGTGT+CGG	+	tig0023826:45352-45371	Msa1411690:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0023826	gene	44469	49574	44469	ID=Msa1411690;Name=Msa1411690
tig0023826	mRNA	44469	49574	44469	ID=Msa1411690-mRNA-1;Parent=Msa1411690;Name=Msa1411690-mRNA-1;_AED=0.04;_eAED=0.04;_QI=69\|1\|1\|1\|1\|1\|7\|277\|228
tig0023826	exon	44469	44655	44469	ID=Msa1411690-mRNA-1:exon:20240;Parent=Msa1411690-mRNA-1
tig0023826	exon	44736	44813	44736	ID=Msa1411690-mRNA-1:exon:20241;Parent=Msa1411690-mRNA-1
tig0023826	exon	44892	44998	44892	ID=Msa1411690-mRNA-1:exon:20242;Parent=Msa1411690-mRNA-1
tig0023826	exon	47509	47586	47509	ID=Msa1411690-mRNA-1:exon:20243;Parent=Msa1411690-mRNA-1
tig0023826	exon	47800	47901	47800	ID=Msa1411690-mRNA-1:exon:20244;Parent=Msa1411690-mRNA-1
tig0023826	exon	48587	48668	48587	ID=Msa1411690-mRNA-1:exon:20245;Parent=Msa1411690-mRNA-1
tig0023826	exon	49176	49574	49176	ID=Msa1411690-mRNA-1:exon:20246;Parent=Msa1411690-mRNA-1
tig0023826	five_prime_UTR	44469	44537	44469	ID=Msa1411690-mRNA-1:five_prime_utr;Parent=Msa1411690-mRNA-1
tig0023826	CDS	44538	44655	44538	ID=Msa1411690-mRNA-1:cds;Parent=Msa1411690-mRNA-1
tig0023826	CDS	44736	44813	44736	ID=Msa1411690-mRNA-1:cds;Parent=Msa1411690-mRNA-1
tig0023826	CDS	44892	44998	44892	ID=Msa1411690-mRNA-1:cds;Parent=Msa1411690-mRNA-1
tig0023826	CDS	47509	47586	47509	ID=Msa1411690-mRNA-1:cds;Parent=Msa1411690-mRNA-1
tig0023826	CDS	47800	47901	47800	ID=Msa1411690-mRNA-1:cds;Parent=Msa1411690-mRNA-1
tig0023826	CDS	48587	48668	48587	ID=Msa1411690-mRNA-1:cds;Parent=Msa1411690-mRNA-1
tig0023826	CDS	49176	49297	49176	ID=Msa1411690-mRNA-1:cds;Parent=Msa1411690-mRNA-1
tig0023826	three_prime_UTR	49298	49574	49298	ID=Msa1411690-mRNA-1:three_prime_utr;Parent=Msa1411690-mRNA-1

Gene Sequence

>Msa1411690

ATGATAGCGGGAAGTGCTACCTTGAAGCTCAATCTCTCCTATTTCTCTCTCCGCAAATCTTCTTCTTCATACCCCTCCAACTTTCCTATTCGCACCCCTTCTAATCCCCGTTTTCTCAAGATTTATGCTATTTCTAGTAATGACATTAAAGTTGGTACTAATATCGAAGTTGATGGTTCACCTTGGCGTGTCTTAGAGTTTCTTCATGTGAAACCTGGAAAAGGTGCTGCATTTGTGAGGACTAAGCTTAAGAATCATCTTACTGGAAATACTGTTGAAAAGACCTTCAGAGCTGGAAGTTCGATTGATGAAGCTGATATAGTCAAGGAAACAAAACAGTTCACATATAAAGATGGTGTTCAATTTGTCTTCATGGACTTGAGTACATACGAGGAGACTCGTCTGGATGAATCAGAAGTTGGGAACAAAACAAAGTGGCTGAAAGAAGGAATGGACTGCAATTTGCTGTTATGGAATGGAAAAGTTATTGATGTTGATCTCCCTATAACAGTCAAGCTTAAGGTAGTCGATGTGGATCCGGGACTTAAGGGTGACACAGCCCAAGGTGGTGGATCAAAGCCAGCAACTCTCGACACTGGTGCTATTGTCAATGTCCCGCTCTTTGTAAATATAGGCGAGGAAATACTGGTTGATACAAGATCTGGGCAGTACATGAGCCGTGCTTAA

Protein sequence

>Msa1411690

MIAGSATLKLNLSYFSLRKSSSSYPSNFPIRTPSNPRFLKIYAISSNDIKVGTNIEVDGSPWRVLEFLHVKPGKGAAFVRTKLKNHLTGNTVEKTFRAGSSIDEADIVKETKQFTYKDGVQFVFMDLSTYEETRLDESEVGNKTKWLKEGMDCNLLLWNGKVIDVDLPITVKLKVVDVDPGLKGDTAQGGGSKPATLDTGAIVNVPLFVNIGEEILVDTRSGQYMSRA*