AlfalfaGEDB | Msa1152500

M. sativa cultivar zhongmu-4 / Msa1152500

KEGG: K01188|K19964
GO: GO:0003674 GO:0003824 GO:0004553 GO:0008150 GO:0008152 GO:0008422 GO:0015926 GO:0016787 GO:0016798 GO:0071704 GO:1901135 GO:1901657
PFAM: PF00232.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1152500	XP_013449165.1	88.247	519	37	1	3	497	1	519	0.0	956

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1152500	sp\|B3H5Q1\|BGL11_ARATH	53.144	493	178	6	29	472	22	510	0.0	536

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1152500	A0A072U0J3	88.247	519	37	1	3	497	1	519	0.0	956

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0008330	Msa1152500	0.800518	1.388389e-48	-8.615850e-47
Msa0016830	Msa1152500	0.807626	4.611185e-50	-8.615850e-47
Msa0021710	Msa1152500	0.817980	2.488631e-52	-8.615850e-47
Msa0026120	Msa1152500	0.820796	5.676872e-53	-8.615850e-47
Msa0030740	Msa1152500	0.805696	1.178421e-49	-8.615850e-47
Msa0034340	Msa1152500	0.824603	7.379036e-54	-8.615850e-47
Msa0035090	Msa1152500	0.803726	3.038433e-49	-8.615850e-47
Msa0039200	Msa1152500	0.858391	8.509904e-63	-8.615850e-47
Msa0040190	Msa1152500	0.828897	6.964129e-55	-8.615850e-47
Msa0046670	Msa1152500	0.819127	1.367075e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1152500	MtrunA17_Chr7g0242691	88.247	519	37	1	3	497	1	519	0.0	956
Msa1152500	MtrunA17_Chr7g0242681	69.866	521	132	2	1	497	6	525	0.0	744
Msa1152500	MtrunA17_Chr4g0006971	64.008	489	152	1	32	496	26	514	0.0	664
Msa1152500	MtrunA17_Chr1g0195611	56.442	489	185	4	34	497	28	513	0.0	557
Msa1152500	MtrunA17_Chr5g0428831	56.122	490	186	5	34	497	28	514	0.0	550
Msa1152500	MtrunA17_Chr1g0195641	55.990	384	141	4	35	393	29	409	3.55e-150	435
Msa1152500	MtrunA17_Chr4g0043091	41.616	495	250	6	15	472	10	502	5.65e-139	410
Msa1152500	MtrunA17_Chr2g0329191	43.841	479	230	6	32	473	33	509	1.33e-134	399
Msa1152500	MtrunA17_Chr2g0329201	42.829	509	251	6	3	473	1	507	8.42e-134	397
Msa1152500	MtrunA17_Chr4g0033271	42.209	507	250	8	5	473	10	511	1.10e-133	396

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1152500	AT1G02850.2	55.864	469	178	4	29	472	22	486	0.0	551
Msa1152500	AT1G02850.5	53.252	492	178	5	29	472	22	509	0.0	540
Msa1152500	AT1G02850.4	53.144	493	178	6	29	472	22	510	0.0	536
Msa1152500	AT1G02850.1	53.305	469	163	5	29	472	22	459	4.72e-179	511
Msa1152500	AT4G27830.1	50.103	487	210	6	14	471	2	484	3.11e-172	495
Msa1152500	AT1G60090.2	49.016	508	217	10	12	486	1	499	2.88e-168	485
Msa1152500	AT4G22100.1	48.317	505	217	8	16	485	3	498	1.31e-167	483
Msa1152500	AT1G45191.2	48.509	503	210	9	12	475	1	493	1.21e-165	478
Msa1152500	AT1G60090.1	47.876	518	215	11	12	486	1	506	2.13e-164	475
Msa1152500	AT1G45191.4	47.904	501	210	9	12	475	1	487	5.02e-164	474

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Find 120 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGACCCATCAATCCTAA+AGG	0.554693	7_4:-38198089	Msa1152500:CDS
AAAGTGCAGGGTATGCAACA+TGG	0.615675	7_4:-38197363	Msa1152500:intron
AAATGATACATCAATAGATG+AGG	0.637241	7_4:-38196658	Msa1152500:intron
AACCTAAGCTCTCTGCTAAA+TGG	0.340098	7_4:-38196037	Msa1152500:CDS
AACTATAAGCACTAGGGAAA+AGG	0.440558	7_4:+38199189	None:intergenic
AAGAACTCAATTGACTTCTT+AGG	0.276518	7_4:-38196973	Msa1152500:CDS
AAGATATAATTGCCACTCAA+AGG	0.508182	7_4:-38197284	Msa1152500:CDS
AAGGAAATATGAGAAGGCAA+AGG	0.622977	7_4:+38195948	None:intergenic
AAGGAAGGAGAACACCTCTG+AGG	0.721271	7_4:+38197606	None:intergenic
AAGGGCCCAAGACTTCATGA+TGG	0.602550	7_4:-38197265	Msa1152500:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CATACTCATTATTGTGCTTA+TGG	+	chr7_4:38195980-38195999	None:intergenic	30.0%
CATAAGCACAATAATGAGTA+TGG	-	chr7_4:38195978-38195997	Msa1152500:CDS	30.0%
TGTTCAAACTATAAGCACTA+GGG	+	chr7_4:38196074-38196093	None:intergenic	30.0%
TTGTTCAAACTATAAGCACT+AGG	+	chr7_4:38196075-38196094	None:intergenic	30.0%
AAAAGAGAGAAGAACAAACT+TGG	+	chr7_4:38196146-38196165	None:intergenic	30.0%
GTAAATACATAGGGATAAGA+TGG	+	chr7_4:38197137-38197156	None:intergenic	30.0%
TACCATCTGGTAAATACATA+GGG	+	chr7_4:38197146-38197165	None:intergenic	30.0%
TTACCATCTGGTAAATACAT+AGG	+	chr7_4:38197147-38197166	None:intergenic	30.0%
ATGTATTTACCAGATGGTAA+AGG	-	chr7_4:38197147-38197166	Msa1152500:intron	30.0%
ATAAATGAACTGATAAGCCA+CGG	-	chr7_4:38197210-38197229	Msa1152500:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_4	gene	38195833	38199443	38195833	ID=Msa1152500;Name=Msa1152500
chr7_4	mRNA	38195833	38199443	38195833	ID=Msa1152500-mRNA-1;Parent=Msa1152500;Name=Msa1152500-mRNA-1;_AED=0.38;_eAED=0.38;_QI=158\|0.90\|0.91\|1\|0.90\|0.91\|12\|108\|497
chr7_4	exon	38195833	38196184	38195833	ID=Msa1152500-mRNA-1:exon:9181;Parent=Msa1152500-mRNA-1
chr7_4	exon	38196290	38196380	38196290	ID=Msa1152500-mRNA-1:exon:9180;Parent=Msa1152500-mRNA-1
chr7_4	exon	38196485	38196572	38196485	ID=Msa1152500-mRNA-1:exon:9179;Parent=Msa1152500-mRNA-1
chr7_4	exon	38196659	38196690	38196659	ID=Msa1152500-mRNA-1:exon:9178;Parent=Msa1152500-mRNA-1
chr7_4	exon	38196875	38197095	38196875	ID=Msa1152500-mRNA-1:exon:9177;Parent=Msa1152500-mRNA-1
chr7_4	exon	38197261	38197376	38197261	ID=Msa1152500-mRNA-1:exon:9176;Parent=Msa1152500-mRNA-1
chr7_4	exon	38197497	38197752	38197497	ID=Msa1152500-mRNA-1:exon:9175;Parent=Msa1152500-mRNA-1
chr7_4	exon	38197881	38197968	38197881	ID=Msa1152500-mRNA-1:exon:9174;Parent=Msa1152500-mRNA-1
chr7_4	exon	38198045	38198116	38198045	ID=Msa1152500-mRNA-1:exon:9173;Parent=Msa1152500-mRNA-1
chr7_4	exon	38198583	38198642	38198583	ID=Msa1152500-mRNA-1:exon:9172;Parent=Msa1152500-mRNA-1
chr7_4	exon	38198747	38198816	38198747	ID=Msa1152500-mRNA-1:exon:9171;Parent=Msa1152500-mRNA-1
chr7_4	exon	38199130	38199443	38199130	ID=Msa1152500-mRNA-1:exon:9170;Parent=Msa1152500-mRNA-1
chr7_4	five_prime_UTR	38199286	38199443	38199286	ID=Msa1152500-mRNA-1:five_prime_utr;Parent=Msa1152500-mRNA-1
chr7_4	CDS	38199130	38199285	38199130	ID=Msa1152500-mRNA-1:cds;Parent=Msa1152500-mRNA-1
chr7_4	CDS	38198747	38198816	38198747	ID=Msa1152500-mRNA-1:cds;Parent=Msa1152500-mRNA-1
chr7_4	CDS	38198583	38198642	38198583	ID=Msa1152500-mRNA-1:cds;Parent=Msa1152500-mRNA-1
chr7_4	CDS	38198045	38198116	38198045	ID=Msa1152500-mRNA-1:cds;Parent=Msa1152500-mRNA-1
chr7_4	CDS	38197881	38197968	38197881	ID=Msa1152500-mRNA-1:cds;Parent=Msa1152500-mRNA-1
chr7_4	CDS	38197497	38197752	38197497	ID=Msa1152500-mRNA-1:cds;Parent=Msa1152500-mRNA-1
chr7_4	CDS	38197261	38197376	38197261	ID=Msa1152500-mRNA-1:cds;Parent=Msa1152500-mRNA-1
chr7_4	CDS	38196875	38197095	38196875	ID=Msa1152500-mRNA-1:cds;Parent=Msa1152500-mRNA-1
chr7_4	CDS	38196659	38196690	38196659	ID=Msa1152500-mRNA-1:cds;Parent=Msa1152500-mRNA-1
chr7_4	CDS	38196485	38196572	38196485	ID=Msa1152500-mRNA-1:cds;Parent=Msa1152500-mRNA-1
chr7_4	CDS	38196290	38196380	38196290	ID=Msa1152500-mRNA-1:cds;Parent=Msa1152500-mRNA-1
chr7_4	CDS	38195941	38196184	38195941	ID=Msa1152500-mRNA-1:cds;Parent=Msa1152500-mRNA-1
chr7_4	three_prime_UTR	38195833	38195940	38195833	ID=Msa1152500-mRNA-1:three_prime_utr;Parent=Msa1152500-mRNA-1

Gene Sequence

>Msa1152500

ATGAGTATGGCTTTGATCTTGAAGAATTTTTCTTTGACAGAATTATTAACTGCTGCAGCAGTGTTAGTAGTAATCCTTTTCCCTAGTGCTTATAGTTTGAACAAAGATGACTTCCCTTCTGACTTTGTGTTTGGTGTATCAACTTCAGCTTACCAATTTGAAGGTGCAGCAAATGAAGATGGCAGGAAACCAAGCATATGGGATACCTTTGCCCATGGTGGAAATGCGAATATGTACAAGGGTGACGGAGATATTGCATGCGATGGATATCACAAATACAAGGTCAATGGTAAAGGACCCATCAATCCTAAAGGACTACAATATTACAACAATCTCATAAATGAACTGATAAGCCACGGAATCCAAGCACATGTTACATTACATCATTGGGATCTACCACAATCACTTGAGGATGAGTATGGAGGATGGGTTAGCGAAAAAATTGTGAAAGATTTCACATCATATGCTGATGTGTGCTTCAAAGAATTTGGCGATCGAGTTAAGTACTGGACCACGGTGAATGAGGTCAATATTTATGCAATGTTTGGCTATGATATTGGAATGGCACCGCCTCAGAGGTGTTCTCCTTCCTTTCTAGCTAACTGCTCAAGGGGAAATTCTTCAACTGAGCCATATTTGGCAGCTCATCATATGTTGTTAGCACATGCTTCAGCTGCAAGATTGTACAGGAAGAAATATAAGGGTATGCAACATGGATTTATTGGGTTTAATCTTCTTACTTTTGGTTTTGTTCCACTTACAAATACTATTGAAGATATAATTGCCACTCAAAGGGCCCAAGACTTCATGATGGGGTGGGTTCTAAATCCATTAACATTTGGTGATTATCCTGATATAATGAAAAAGAATGTTGGATCAAGGCTTCCTTCCTTCACAGTAAAGGAATCAAATCTGATCAAGAACTCAATTGACTTCTTAGGAGTAAACTTTTACAACTTAATATATGTTCAGAACAATCCTGCAAGCCTGAACATGGAGGATAGAGATTACATGCAAGATATGGCAGTGGAAATTACAATCATGATAGAAAATGATACATCAATAGATGAGTTTCCGATTACAACCAAGAATTTTCAAGAGGTGCTATATTACTTGAAGAATGATTATGGAAATTTTCCAATTTACATACATGAAAATGGCTACAGAACACTTCGCAATTCATCCTTGAATGATTGGTCTAGGGTGAAGTACTTGCATAAATACATTGGGACCATAGCTAATGAAATAAGGAGTGGATCGAATATAAAAGGTTATTTTGTATGGTCCTTCGTGGATGTGCTTGAAATACTGAGTGGATATGAAGAAAGTTTTGGCCTATACTACATAGATATGAATGATGCAAACTTGCAGAGACAACCTAAGCTCTCTGCTAAATGGTACTCAAATTTTCTGAAAAGGAAACCTATGGACTCAAAGATTATTATGGAATTTGAGAATGATTCCCCTTTGCCTTCTCATATTTCCTTGCTATAA

Protein sequence

>Msa1152500

MSMALILKNFSLTELLTAAAVLVVILFPSAYSLNKDDFPSDFVFGVSTSAYQFEGAANEDGRKPSIWDTFAHGGNANMYKGDGDIACDGYHKYKVNGKGPINPKGLQYYNNLINELISHGIQAHVTLHHWDLPQSLEDEYGGWVSEKIVKDFTSYADVCFKEFGDRVKYWTTVNEVNIYAMFGYDIGMAPPQRCSPSFLANCSRGNSSTEPYLAAHHMLLAHASAARLYRKKYKGMQHGFIGFNLLTFGFVPLTNTIEDIIATQRAQDFMMGWVLNPLTFGDYPDIMKKNVGSRLPSFTVKESNLIKNSIDFLGVNFYNLIYVQNNPASLNMEDRDYMQDMAVEITIMIENDTSIDEFPITTKNFQEVLYYLKNDYGNFPIYIHENGYRTLRNSSLNDWSRVKYLHKYIGTIANEIRSGSNIKGYFVWSFVDVLEILSGYEESFGLYYIDMNDANLQRQPKLSAKWYSNFLKRKPMDSKIIMEFENDSPLPSHISLL*