AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0344270


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0344270 MtrunA17_CPg0492821 100.000 83 0 0 1 83 1 83 5.28e-56 167
Msa0344270 MtrunA17_Chr4g0016851 100.000 83 0 0 1 83 1 83 5.28e-56 167
Msa0344270 MtrunA17_Chr4g0015751 100.000 83 0 0 1 83 1 83 5.28e-56 167
Msa0344270 MtrunA17_Chr4g0017011 97.468 79 2 0 1 79 1 79 4.69e-52 157
Msa0344270 MtrunA17_Chr5g0423051 98.684 76 1 0 1 76 1 76 1.14e-49 151
Msa0344270 MtrunA17_Chr6g0451331 94.737 38 2 0 24 61 41 78 2.48e-20 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0344270 ATCG00580.1 97.590 83 2 0 1 83 1 83 6.85e-55 164

Find 22 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTAGCTTACGATGTATT+TGG 0.244623 3_1:+2739388 Msa0344270:CDS
TCAAAACGGCCAGTTATTAA+TGG 0.307608 3_1:-2739457 None:intergenic
ATAACCAACCCGCAATGAAT+AGG 0.348030 3_1:-2739354 None:intergenic
TTATTACCAGTATTCGATAC+TGG 0.348418 3_1:+2739305 Msa0344270:CDS
TATGTGGAGCTCGGCATGTC+TGG 0.349487 3_1:+2739253 None:intergenic
ATACCCTCCCTATTCATTGC+GGG 0.391873 3_1:+2739346 Msa0344270:CDS
TAACCAACCCGCAATGAATA+GGG 0.406851 3_1:-2739353 None:intergenic
CTTTCTGTAAAATACTCGTT+TGG 0.412027 3_1:-2739421 None:intergenic
CAAGGAATTCCATTAATAAC+TGG 0.412082 3_1:+2739448 Msa0344270:CDS
AATAATCCAGTATCGAATAC+TGG 0.423687 3_1:-2739311 None:intergenic
TTATTAATGGAATTCCTTGT+CGG 0.434610 3_1:-2739444 None:intergenic
CTCGGCATGTCTGGAAGCAC+AGG 0.508926 3_1:+2739262 None:intergenic
GGTTGGTTATTTGTCAGCAC+GGG 0.523918 3_1:+2739367 Msa0344270:CDS
TGTAAAATACTCGTTTGGAC+GGG 0.525534 3_1:-2739416 None:intergenic
GTAAAATACTCGTTTGGACG+GGG 0.531710 3_1:-2739415 None:intergenic
GGGTTGGTTATTTGTCAGCA+CGG 0.532372 3_1:+2739366 Msa0344270:CDS
CCTCCCTATTCATTGCGGGT+TGG 0.536548 3_1:+2739350 Msa0344270:CDS
CTGTAAAATACTCGTTTGGA+CGG 0.550313 3_1:-2739417 None:intergenic
GTTGTTCCAAAGAATCAAAA+CGG 0.551629 3_1:-2739471 None:intergenic
TATACCCTCCCTATTCATTG+CGG 0.586067 3_1:+2739345 Msa0344270:CDS
CAACCCGCAATGAATAGGGA+GGG 0.665638 3_1:-2739349 None:intergenic
CCAACCCGCAATGAATAGGG+AGG 0.678708 3_1:-2739350 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTATTAATGGAATTCCTTGT+CGG - chr3_1:2739447-2739466 None:intergenic 25.0%
TTATTACCAGTATTCGATAC+TGG + chr3_1:2739305-2739324 Msa0344270:CDS 30.0%
! AATAATCCAGTATCGAATAC+TGG - chr3_1:2739314-2739333 None:intergenic 30.0%
CTTTCTGTAAAATACTCGTT+TGG - chr3_1:2739424-2739443 None:intergenic 30.0%
CAAGGAATTCCATTAATAAC+TGG + chr3_1:2739448-2739467 Msa0344270:CDS 30.0%
GTTGTTCCAAAGAATCAAAA+CGG - chr3_1:2739474-2739493 None:intergenic 30.0%
! GGTTTAGCTTACGATGTATT+TGG + chr3_1:2739388-2739407 Msa0344270:CDS 35.0%
TGTAAAATACTCGTTTGGAC+GGG - chr3_1:2739419-2739438 None:intergenic 35.0%
CTGTAAAATACTCGTTTGGA+CGG - chr3_1:2739420-2739439 None:intergenic 35.0%
! TATTTTACAGAAAGCCGACA+AGG + chr3_1:2739430-2739449 Msa0344270:CDS 35.0%
TCAAAACGGCCAGTTATTAA+TGG - chr3_1:2739460-2739479 None:intergenic 35.0%
TATACCCTCCCTATTCATTG+CGG + chr3_1:2739345-2739364 Msa0344270:CDS 40.0%
TAACCAACCCGCAATGAATA+GGG - chr3_1:2739356-2739375 None:intergenic 40.0%
ATAACCAACCCGCAATGAAT+AGG - chr3_1:2739357-2739376 None:intergenic 40.0%
GTAAAATACTCGTTTGGACG+GGG - chr3_1:2739418-2739437 None:intergenic 40.0%
!!! AACTGGCCGTTTTGATTCTT+TGG + chr3_1:2739465-2739484 Msa0344270:CDS 40.0%
ATACCCTCCCTATTCATTGC+GGG + chr3_1:2739346-2739365 Msa0344270:CDS 45.0%
!! GGGTTGGTTATTTGTCAGCA+CGG + chr3_1:2739366-2739385 Msa0344270:CDS 45.0%
! GGTTGGTTATTTGTCAGCAC+GGG + chr3_1:2739367-2739386 Msa0344270:CDS 45.0%
CAACCCGCAATGAATAGGGA+GGG - chr3_1:2739352-2739371 None:intergenic 50.0%
CCAACCCGCAATGAATAGGG+AGG - chr3_1:2739353-2739372 None:intergenic 55.0%
CCTCCCTATTCATTGCGGGT+TGG + chr3_1:2739350-2739369 Msa0344270:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 2739268 2739519 2739268 ID=Msa0344270;Name=Msa0344270
chr3_1 mRNA 2739268 2739519 2739268 ID=Msa0344270-mRNA-1;Parent=Msa0344270;Name=Msa0344270-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|83
chr3_1 exon 2739268 2739519 2739268 ID=Msa0344270-mRNA-1:exon:835;Parent=Msa0344270-mRNA-1
chr3_1 CDS 2739268 2739519 2739268 ID=Msa0344270-mRNA-1:cds;Parent=Msa0344270-mRNA-1
Gene Sequence

>Msa0344270

ATGTCTGGAAGCACAGGAGAACGTTCTTTTGCTGATATTATTACCAGTATTCGATACTGGATTATTCATAGCATTACTATACCCTCCCTATTCATTGCGGGTTGGTTATTTGTCAGCACGGGTTTAGCTTACGATGTATTTGGAAGTCCCCGTCCAAACGAGTATTTTACAGAAAGCCGACAAGGAATTCCATTAATAACTGGCCGTTTTGATTCTTTGGAACAACTCGATGAATTTAGTAGATCTTTTTAG

Protein sequence

>Msa0344270

MSGSTGERSFADIITSIRYWIIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNEYFTESRQGIPLITGRFDSLEQLDEFSRSF*