AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0344220


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0344220 MtrunA17_CPg0492821 100.000 83 0 0 1 83 1 83 5.28e-56 167
Msa0344220 MtrunA17_Chr4g0016851 100.000 83 0 0 1 83 1 83 5.28e-56 167
Msa0344220 MtrunA17_Chr4g0015751 100.000 83 0 0 1 83 1 83 5.28e-56 167
Msa0344220 MtrunA17_Chr4g0017011 97.468 79 2 0 1 79 1 79 4.69e-52 157
Msa0344220 MtrunA17_Chr5g0423051 98.684 76 1 0 1 76 1 76 1.14e-49 151
Msa0344220 MtrunA17_Chr6g0451331 94.737 38 2 0 24 61 41 78 2.48e-20 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0344220 ATCG00580.1 97.590 83 2 0 1 83 1 83 6.85e-55 164

Find 22 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTAGCTTACGATGTATT+TGG 0.244623 3_1:-2717603 Msa0344220:CDS
TCAAAACGGCCAGTTATTAA+TGG 0.307608 3_1:+2717534 None:intergenic
ATAACCAACCCGCAATGAAT+AGG 0.348030 3_1:+2717637 None:intergenic
TTATTACCAGTATTCGATAC+TGG 0.348418 3_1:-2717686 Msa0344220:CDS
TATGTGGAGCTCGGCATGTC+TGG 0.349487 3_1:-2717738 None:intergenic
ATACCCTCCCTATTCATTGC+GGG 0.391873 3_1:-2717645 Msa0344220:CDS
TAACCAACCCGCAATGAATA+GGG 0.406851 3_1:+2717638 None:intergenic
CTTTCTGTAAAATACTCGTT+TGG 0.412027 3_1:+2717570 None:intergenic
CAAGGAATTCCATTAATAAC+TGG 0.412082 3_1:-2717543 Msa0344220:CDS
AATAATCCAGTATCGAATAC+TGG 0.423687 3_1:+2717680 None:intergenic
TTATTAATGGAATTCCTTGT+CGG 0.434610 3_1:+2717547 None:intergenic
CTCGGCATGTCTGGAAGCAC+AGG 0.508926 3_1:-2717729 None:intergenic
GGTTGGTTATTTGTCAGCAC+GGG 0.523918 3_1:-2717624 Msa0344220:CDS
TGTAAAATACTCGTTTGGAC+GGG 0.525534 3_1:+2717575 None:intergenic
GTAAAATACTCGTTTGGACG+GGG 0.531710 3_1:+2717576 None:intergenic
GGGTTGGTTATTTGTCAGCA+CGG 0.532372 3_1:-2717625 Msa0344220:CDS
CCTCCCTATTCATTGCGGGT+TGG 0.536548 3_1:-2717641 Msa0344220:CDS
CTGTAAAATACTCGTTTGGA+CGG 0.550313 3_1:+2717574 None:intergenic
GTTGTTCCAAAGAATCAAAA+CGG 0.551629 3_1:+2717520 None:intergenic
TATACCCTCCCTATTCATTG+CGG 0.586067 3_1:-2717646 Msa0344220:CDS
CAACCCGCAATGAATAGGGA+GGG 0.665638 3_1:+2717642 None:intergenic
CCAACCCGCAATGAATAGGG+AGG 0.678708 3_1:+2717641 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTATTAATGGAATTCCTTGT+CGG + chr3_1:2717673-2717692 None:intergenic 25.0%
TTATTACCAGTATTCGATAC+TGG - chr3_1:2717531-2717550 Msa0344220:CDS 30.0%
! AATAATCCAGTATCGAATAC+TGG + chr3_1:2717540-2717559 None:intergenic 30.0%
CTTTCTGTAAAATACTCGTT+TGG + chr3_1:2717650-2717669 None:intergenic 30.0%
CAAGGAATTCCATTAATAAC+TGG - chr3_1:2717674-2717693 Msa0344220:CDS 30.0%
GTTGTTCCAAAGAATCAAAA+CGG + chr3_1:2717700-2717719 None:intergenic 30.0%
! GGTTTAGCTTACGATGTATT+TGG - chr3_1:2717614-2717633 Msa0344220:CDS 35.0%
TGTAAAATACTCGTTTGGAC+GGG + chr3_1:2717645-2717664 None:intergenic 35.0%
CTGTAAAATACTCGTTTGGA+CGG + chr3_1:2717646-2717665 None:intergenic 35.0%
! TATTTTACAGAAAGCCGACA+AGG - chr3_1:2717656-2717675 Msa0344220:CDS 35.0%
TCAAAACGGCCAGTTATTAA+TGG + chr3_1:2717686-2717705 None:intergenic 35.0%
TATACCCTCCCTATTCATTG+CGG - chr3_1:2717571-2717590 Msa0344220:CDS 40.0%
TAACCAACCCGCAATGAATA+GGG + chr3_1:2717582-2717601 None:intergenic 40.0%
ATAACCAACCCGCAATGAAT+AGG + chr3_1:2717583-2717602 None:intergenic 40.0%
GTAAAATACTCGTTTGGACG+GGG + chr3_1:2717644-2717663 None:intergenic 40.0%
!!! AACTGGCCGTTTTGATTCTT+TGG - chr3_1:2717691-2717710 Msa0344220:CDS 40.0%
ATACCCTCCCTATTCATTGC+GGG - chr3_1:2717572-2717591 Msa0344220:CDS 45.0%
!! GGGTTGGTTATTTGTCAGCA+CGG - chr3_1:2717592-2717611 Msa0344220:CDS 45.0%
! GGTTGGTTATTTGTCAGCAC+GGG - chr3_1:2717593-2717612 Msa0344220:CDS 45.0%
CAACCCGCAATGAATAGGGA+GGG + chr3_1:2717578-2717597 None:intergenic 50.0%
CCAACCCGCAATGAATAGGG+AGG + chr3_1:2717579-2717598 None:intergenic 55.0%
CCTCCCTATTCATTGCGGGT+TGG - chr3_1:2717576-2717595 Msa0344220:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 2717494 2717745 2717494 ID=Msa0344220;Name=Msa0344220
chr3_1 mRNA 2717494 2717745 2717494 ID=Msa0344220-mRNA-1;Parent=Msa0344220;Name=Msa0344220-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|83
chr3_1 exon 2717494 2717745 2717494 ID=Msa0344220-mRNA-1:exon:865;Parent=Msa0344220-mRNA-1
chr3_1 CDS 2717494 2717745 2717494 ID=Msa0344220-mRNA-1:cds;Parent=Msa0344220-mRNA-1
Gene Sequence

>Msa0344220

ATGTCTGGAAGCACAGGAGAACGTTCTTTTGCTGATATTATTACCAGTATTCGATACTGGATTATTCATAGCATTACTATACCCTCCCTATTCATTGCGGGTTGGTTATTTGTCAGCACGGGTTTAGCTTACGATGTATTTGGAAGTCCCCGTCCAAACGAGTATTTTACAGAAAGCCGACAAGGAATTCCATTAATAACTGGCCGTTTTGATTCTTTGGAACAACTCGATGAATTTAGTAGATCTTTTTAG

Protein sequence

>Msa0344220

MSGSTGERSFADIITSIRYWIIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNEYFTESRQGIPLITGRFDSLEQLDEFSRSF*