AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0589640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0589640 MtrunA17_Chr4g0006851 96.316 380 14 0 1 380 1 380 0.0 710
Msa0589640 MtrunA17_Chr7g0242311 76.842 380 80 3 1 377 1 375 0.0 571
Msa0589640 MtrunA17_Chr4g0063561 35.915 284 164 7 89 362 123 398 8.94e-52 177
Msa0589640 MtrunA17_Chr4g0063561 30.857 175 114 4 188 356 122 295 2.69e-15 76.6
Msa0589640 MtrunA17_Chr7g0240341 33.016 315 187 8 70 364 10 320 4.16e-49 168
Msa0589640 MtrunA17_Chr1g0149111 35.915 284 156 7 89 358 139 410 9.56e-48 167
Msa0589640 MtrunA17_Chr1g0149111 27.011 174 123 1 188 357 138 311 1.74e-16 80.5
Msa0589640 MtrunA17_Chr3g0137941 35.315 286 163 8 91 364 124 399 5.38e-47 165
Msa0589640 MtrunA17_Chr3g0137941 32.461 191 108 5 87 267 214 393 8.95e-18 84.3
Msa0589640 MtrunA17_Chr3g0137941 26.012 173 124 1 188 356 121 293 6.96e-16 78.6
Msa0589640 MtrunA17_Chr8g0347451 31.731 312 164 7 89 359 51 354 2.08e-45 159
Msa0589640 MtrunA17_Chr5g0396801 32.573 307 166 6 89 359 38 339 5.46e-45 158
Msa0589640 MtrunA17_Chr3g0135861 32.381 315 174 6 82 364 28 335 1.08e-43 154
Msa0589640 MtrunA17_Chr3g0135861 26.190 210 133 4 89 277 131 339 1.18e-15 77.4
Msa0589640 MtrunA17_Chr3g0131501 31.250 304 176 8 76 356 185 478 2.12e-43 157
Msa0589640 MtrunA17_Chr3g0131501 31.053 190 118 3 86 267 296 480 8.10e-20 90.9
Msa0589640 MtrunA17_Chr6g0488081 30.368 326 168 7 65 355 2 303 3.09e-41 147
Msa0589640 MtrunA17_Chr8g0385571 31.818 286 165 7 89 356 227 500 8.23e-41 150
Msa0589640 MtrunA17_Chr8g0385571 31.053 190 118 3 86 267 318 502 9.36e-21 93.6
Msa0589640 MtrunA17_Chr2g0296061 29.703 303 173 7 91 353 17 319 3.02e-40 145
Msa0589640 MtrunA17_Chr2g0296061 27.751 209 125 5 190 372 16 224 1.46e-16 80.1
Msa0589640 MtrunA17_Chr2g0296061 25.000 204 121 4 89 267 128 324 7.09e-14 72.0
Msa0589640 MtrunA17_Chr5g0436041 34.828 290 169 7 89 359 34 322 2.18e-39 142
Msa0589640 MtrunA17_Chr5g0436041 30.890 191 114 6 89 269 141 323 2.49e-11 64.3
Msa0589640 MtrunA17_Chr4g0067231 28.387 310 180 7 91 358 16 325 2.07e-38 140
Msa0589640 MtrunA17_Chr4g0067231 28.774 212 122 6 190 372 15 226 2.20e-18 85.1
Msa0589640 MtrunA17_Chr4g0067231 26.214 206 116 6 89 267 129 325 3.53e-14 72.8
Msa0589640 MtrunA17_Chr7g0252401 32.270 282 166 9 89 356 207 477 6.22e-38 142
Msa0589640 MtrunA17_Chr7g0252401 30.769 182 118 2 89 267 303 479 7.23e-23 99.8
Msa0589640 MtrunA17_Chr7g0252401 26.966 178 121 3 189 357 207 384 4.80e-12 67.4
Msa0589640 MtrunA17_Chr3g0135801 32.646 291 172 8 89 358 34 321 1.84e-36 135
Msa0589640 MtrunA17_Chr3g0115801 35.034 294 151 9 91 355 118 400 1.25e-34 132
Msa0589640 MtrunA17_Chr3g0115801 27.083 192 134 3 188 374 115 305 9.47e-15 75.1
Msa0589640 MtrunA17_Chr8g0393031 30.420 286 175 10 94 357 20 303 9.55e-32 122
Msa0589640 MtrunA17_Chr8g0382201 32.881 295 156 10 91 355 197 479 1.66e-31 124
Msa0589640 MtrunA17_Chr8g0382201 27.041 196 131 3 172 355 170 365 1.79e-13 71.6
Msa0589640 MtrunA17_Chr4g0000261 26.936 297 181 7 89 358 66 353 1.55e-27 111
Msa0589640 MtrunA17_Chr4g0000261 25.521 192 122 2 186 356 63 254 2.11e-14 73.9
Msa0589640 MtrunA17_Chr8g0352031 32.226 301 178 9 75 352 52 349 5.23e-27 110
Msa0589640 MtrunA17_Chr7g0269701 30.000 290 178 10 91 360 24 308 2.00e-26 107
Msa0589640 MtrunA17_Chr7g0269701 28.571 189 118 5 190 362 23 210 6.82e-15 74.7
Msa0589640 MtrunA17_Chr7g0269701 28.729 181 110 6 103 271 137 310 2.53e-11 64.3
Msa0589640 MtrunA17_Chr6g0488341 28.185 259 133 6 134 364 4 237 9.66e-26 103
Msa0589640 MtrunA17_Chr3g0088301 31.724 290 175 9 89 358 93 379 1.58e-25 106
Msa0589640 MtrunA17_Chr4g0040341 33.099 284 167 9 89 352 75 355 7.38e-25 104
Msa0589640 MtrunA17_Chr1g0195101 28.826 281 173 11 101 360 31 305 1.49e-23 99.8
Msa0589640 MtrunA17_Chr2g0328891 32.069 290 174 9 89 358 66 352 5.21e-23 99.0
Msa0589640 MtrunA17_Chr4g0008061 24.742 291 196 7 89 365 15 296 1.90e-22 96.3
Msa0589640 MtrunA17_Chr7g0241521 24.055 291 198 7 89 365 17 298 3.00e-22 95.9
Msa0589640 MtrunA17_Chr7g0241521 25.500 200 128 3 180 358 8 207 5.19e-12 66.2
Msa0589640 MtrunA17_Chr5g0423111 26.263 297 188 8 101 377 33 318 1.69e-21 94.7
Msa0589640 MtrunA17_Chr8g0372531 27.465 284 178 9 105 363 30 310 1.19e-20 91.7
Msa0589640 MtrunA17_Chr5g0431531 26.855 283 189 6 88 358 38 314 2.74e-20 90.5
Msa0589640 MtrunA17_Chr8g0386871 26.429 280 184 7 90 359 321 588 1.16e-19 90.9
Msa0589640 MtrunA17_Chr8g0386871 26.842 190 120 7 191 368 322 504 6.52e-13 70.1
Msa0589640 MtrunA17_Chr3g0086811 25.000 308 183 9 89 355 28 328 4.64e-18 84.7
Msa0589640 MtrunA17_Chr8g0383321 23.913 276 182 6 90 359 53 306 1.89e-17 82.4
Msa0589640 MtrunA17_Chr8g0383321 29.255 188 111 5 91 270 135 308 8.21e-15 74.7
Msa0589640 MtrunA17_Chr1g0201361 24.275 276 181 6 90 359 52 305 3.06e-17 81.6
Msa0589640 MtrunA17_Chr1g0201361 28.638 213 132 6 64 271 111 308 1.01e-13 71.2
Msa0589640 MtrunA17_Chr6g0463041 27.313 227 109 5 189 364 25 246 7.99e-17 79.3
Msa0589640 MtrunA17_Chr6g0463041 23.041 217 128 4 89 267 25 240 5.99e-12 65.1
Msa0589640 MtrunA17_Chr4g0028531 22.373 295 200 8 90 365 23 307 1.74e-16 79.3
Msa0589640 MtrunA17_Chr3g0103681 25.000 264 180 8 89 336 15 276 5.37e-16 77.8
Msa0589640 MtrunA17_Chr3g0133111 24.262 305 196 7 89 358 6 310 2.69e-15 75.9
Msa0589640 MtrunA17_Chr3g0133111 28.125 192 120 6 91 269 126 312 1.22e-11 65.1
Msa0589640 MtrunA17_Chr5g0411411 25.517 290 193 8 91 359 11 298 6.09e-15 74.7
Msa0589640 MtrunA17_Chr5g0411411 25.439 228 156 4 50 267 74 297 7.11e-13 68.6
Msa0589640 MtrunA17_Chr8g0343381 27.604 192 112 4 189 358 35 221 1.04e-14 74.7
Msa0589640 MtrunA17_Chr1g0196921 20.000 290 206 6 93 362 18 301 1.44e-14 73.6
Msa0589640 MtrunA17_Chr1g0196921 25.000 184 130 3 90 265 112 295 7.71e-13 68.6
Msa0589640 MtrunA17_Chr8g0366791 30.159 189 122 3 89 267 109 297 2.07e-14 73.2
Msa0589640 MtrunA17_Chr8g0366791 25.743 303 191 11 82 359 5 298 1.44e-13 70.9
Msa0589640 MtrunA17_Chr2g0293621 25.256 293 186 10 92 357 32 318 1.71e-13 70.9
Msa0589640 MtrunA17_Chr4g0004571 24.315 292 192 8 89 362 16 296 8.46e-13 68.6
Msa0589640 MtrunA17_Chr6g0463351 25.836 329 207 10 28 340 16 323 1.57e-12 68.2
Msa0589640 MtrunA17_Chr5g0412401 22.930 314 198 8 89 358 6 319 3.55e-12 66.6
Msa0589640 MtrunA17_Chr1g0200171 28.276 145 102 2 133 275 648 792 4.17e-12 67.8
Msa0589640 MtrunA17_Chr1g0200171 21.944 319 216 11 60 363 489 789 2.58e-11 65.5
Msa0589640 MtrunA17_Chr7g0219891 25.424 236 148 7 46 265 85 308 5.51e-12 66.2
Msa0589640 MtrunA17_Chr7g0219891 27.027 185 118 6 189 358 23 205 6.46e-11 62.8
Msa0589640 MtrunA17_Chr6g0461981 23.392 171 123 4 195 358 70 239 5.69e-12 66.6
Msa0589640 MtrunA17_Chr3g0136041 26.020 196 120 3 189 361 34 227 8.97e-12 65.9
Msa0589640 MtrunA17_Chr8g0385891 24.859 177 124 5 190 358 74 249 1.21e-11 65.5
Msa0589640 MtrunA17_Chr2g0331771 20.833 288 200 6 89 358 156 433 5.81e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0589640 AT3G51870.1 67.914 374 116 1 6 375 4 377 0.0 509
Msa0589640 AT5G01500.1 76.994 326 75 0 50 375 80 405 2.89e-176 497
Msa0589640 AT4G32400.1 34.495 287 168 7 89 364 114 391 2.30e-48 168
Msa0589640 AT4G32400.1 27.686 242 150 6 39 273 168 391 3.86e-19 88.6
Msa0589640 AT4G32400.1 28.177 181 126 1 188 364 113 293 2.59e-18 86.3
Msa0589640 AT2G37890.1 34.021 291 172 6 89 360 45 334 2.28e-47 164
Msa0589640 AT2G37890.1 31.606 193 113 6 89 269 152 337 5.50e-16 78.6
Msa0589640 AT4G01100.1 31.715 309 168 5 89 359 42 345 8.36e-47 163
Msa0589640 AT4G01100.1 32.536 209 108 5 91 272 147 349 1.61e-21 95.1
Msa0589640 AT4G26180.1 32.209 326 187 5 77 374 9 328 3.92e-46 161
Msa0589640 AT3G55640.1 35.570 298 158 8 84 355 33 322 8.05e-46 160
Msa0589640 AT3G55640.4 35.570 298 158 8 84 355 33 322 8.05e-46 160
Msa0589640 AT4G01100.2 30.368 326 164 6 89 359 42 359 1.27e-44 158
Msa0589640 AT4G01100.2 32.536 209 108 5 91 272 161 363 2.16e-21 94.7
Msa0589640 AT3G53940.1 33.333 291 172 7 89 359 73 361 7.05e-44 156
Msa0589640 AT4G01100.3 31.438 299 162 6 99 359 2 295 1.25e-43 154
Msa0589640 AT4G01100.3 32.536 209 108 5 91 272 97 299 7.15e-22 95.1
Msa0589640 AT4G26180.2 32.986 288 162 4 77 339 9 290 1.55e-41 148
Msa0589640 AT1G14560.1 31.864 295 165 4 100 364 38 326 4.48e-41 147
Msa0589640 AT5G61810.2 32.155 283 168 5 88 359 64 333 1.58e-40 146
Msa0589640 AT5G48970.1 29.739 306 172 7 91 353 24 329 5.72e-40 145
Msa0589640 AT5G48970.1 29.767 215 116 6 190 379 23 227 6.20e-20 90.1
Msa0589640 AT5G48970.1 24.880 209 118 5 89 267 135 334 1.16e-12 68.9
Msa0589640 AT5G61810.1 31.930 285 170 5 86 359 205 476 2.26e-39 146
Msa0589640 AT1G14560.2 32.963 270 145 4 100 339 38 301 1.81e-38 140
Msa0589640 AT5G51050.1 33.898 295 154 11 84 356 218 493 3.63e-38 143
Msa0589640 AT5G51050.1 30.769 182 119 3 89 267 318 495 4.10e-20 92.0
Msa0589640 AT5G51050.1 26.705 176 120 2 191 357 225 400 7.06e-12 67.0
Msa0589640 AT3G21390.1 29.841 315 175 8 91 361 21 333 4.04e-37 137
Msa0589640 AT3G21390.1 28.000 200 121 4 190 366 20 219 2.48e-18 85.5
Msa0589640 AT3G21390.1 26.733 202 114 4 89 262 130 325 3.94e-12 67.4
Msa0589640 AT3G21390.2 29.841 315 175 8 91 361 43 355 6.85e-37 137
Msa0589640 AT3G21390.2 28.000 200 121 4 190 366 42 241 3.88e-18 85.1
Msa0589640 AT3G21390.2 26.733 202 114 4 89 262 152 347 4.19e-12 67.4
Msa0589640 AT3G20240.1 31.475 305 173 8 84 361 47 342 8.99e-37 137
Msa0589640 AT5G07320.1 31.250 272 163 6 99 359 219 477 3.70e-34 132
Msa0589640 AT3G55640.2 37.113 194 106 4 84 264 33 223 1.25e-32 123
Msa0589640 AT3G55640.3 37.113 194 106 4 84 264 33 223 1.25e-32 123
Msa0589640 AT5G64970.1 32.095 296 157 10 91 355 137 419 1.84e-32 126
Msa0589640 AT5G64970.1 28.652 178 121 3 183 355 129 305 8.04e-13 69.7
Msa0589640 AT5G56450.1 30.169 295 178 8 89 358 34 325 3.31e-32 124
Msa0589640 AT5G56450.1 28.571 161 106 3 87 241 143 300 3.23e-11 64.3
Msa0589640 AT5G48970.2 28.794 257 148 6 132 353 2 258 8.36e-32 121
Msa0589640 AT5G48970.2 30.120 166 98 4 222 379 1 156 3.00e-16 78.6
Msa0589640 AT5G48970.2 24.880 209 118 5 89 267 64 263 1.03e-12 68.2
Msa0589640 AT5G64970.2 30.976 297 161 10 90 355 12 295 1.31e-31 122
Msa0589640 AT3G53940.2 35.638 188 107 4 89 264 73 258 1.07e-30 119
Msa0589640 AT3G53940.2 29.126 206 119 7 173 355 57 258 4.56e-13 69.7
Msa0589640 AT5G64970.3 32.143 280 146 10 91 339 137 403 5.54e-29 116
Msa0589640 AT5G64970.3 28.652 178 121 3 183 355 129 305 7.28e-13 69.7
Msa0589640 AT5G17400.1 29.773 309 180 12 76 357 1 299 5.09e-27 109
Msa0589640 AT1G25380.1 27.852 298 188 7 101 379 33 322 4.54e-26 107
Msa0589640 AT1G25380.2 27.852 298 188 7 101 379 33 322 4.54e-26 107
Msa0589640 AT1G78180.1 32.313 294 159 10 91 355 130 412 5.68e-26 108
Msa0589640 AT1G78180.1 29.651 172 117 2 188 355 127 298 8.45e-13 69.7
Msa0589640 AT3G08580.2 28.446 341 212 10 47 358 34 371 1.86e-25 106
Msa0589640 AT3G08580.1 28.446 341 212 10 47 358 34 371 1.86e-25 106
Msa0589640 AT2G30160.1 27.358 318 206 7 60 358 10 321 1.95e-25 105
Msa0589640 AT2G30160.1 22.330 206 156 1 175 376 28 233 8.65e-14 72.0
Msa0589640 AT1G14560.3 30.088 226 128 3 169 364 3 228 2.23e-25 103
Msa0589640 AT1G14560.3 30.500 200 116 5 89 267 25 222 6.40e-18 82.4
Msa0589640 AT5G13490.1 30.345 290 180 8 89 358 88 375 4.73e-25 105
Msa0589640 AT5G13490.2 30.345 290 180 8 89 358 88 375 4.73e-25 105
Msa0589640 AT4G28390.1 30.796 289 179 8 89 358 83 369 2.86e-24 103
Msa0589640 AT4G28390.2 30.796 289 179 8 89 358 83 369 2.86e-24 103
Msa0589640 AT2G47490.1 29.818 275 173 8 101 358 29 300 7.78e-24 100
Msa0589640 AT2G47490.1 27.273 176 109 6 200 359 29 201 7.66e-11 63.2
Msa0589640 AT1G07030.1 28.169 284 185 6 88 358 39 316 4.84e-23 99.0
Msa0589640 AT3G54110.1 24.000 300 206 7 89 374 15 306 4.74e-22 95.9
Msa0589640 AT4G03115.1 26.866 268 184 6 72 332 21 283 1.95e-21 94.0
Msa0589640 AT4G03115.3 26.493 268 185 5 72 332 53 315 2.41e-21 94.4
Msa0589640 AT5G66380.1 30.137 292 177 11 91 358 12 300 5.97e-21 92.8
Msa0589640 AT4G39460.2 26.978 278 173 7 89 359 58 312 1.16e-19 89.4
Msa0589640 AT4G39460.2 30.734 218 119 7 64 270 118 314 2.84e-17 82.4
Msa0589640 AT4G39460.1 26.978 278 173 7 89 359 58 312 1.16e-19 89.4
Msa0589640 AT4G39460.1 30.734 218 119 7 64 270 118 314 2.84e-17 82.4
Msa0589640 AT4G39460.3 26.978 278 173 7 89 359 58 312 1.16e-19 89.4
Msa0589640 AT4G39460.3 30.734 218 119 7 64 270 118 314 2.84e-17 82.4
Msa0589640 AT1G34065.1 26.950 282 178 6 89 364 82 341 3.27e-19 88.2
Msa0589640 AT1G34065.2 26.950 282 178 6 89 364 75 334 3.40e-19 88.2
Msa0589640 AT5G58970.1 23.675 283 201 5 89 357 16 297 4.80e-19 87.0
Msa0589640 AT4G03115.5 27.778 216 144 5 124 332 2 212 1.50e-18 84.7
Msa0589640 AT4G11440.1 26.316 285 184 9 90 359 331 604 9.00e-18 85.5
Msa0589640 AT4G11440.2 26.316 285 184 9 90 359 331 604 9.00e-18 85.5
Msa0589640 AT4G03115.6 27.014 211 146 4 129 332 11 220 1.05e-17 82.4
Msa0589640 AT1G14140.1 24.407 295 192 9 90 361 17 303 2.47e-17 82.4
Msa0589640 AT4G03115.4 29.146 199 136 3 72 270 21 214 3.72e-17 80.1
Msa0589640 AT4G03115.4 25.301 166 116 4 199 358 48 211 8.35e-11 62.0
Msa0589640 AT4G03115.2 29.146 199 136 3 72 270 21 214 4.49e-17 80.1
Msa0589640 AT4G03115.2 25.301 166 116 4 199 358 48 211 5.92e-11 62.4
Msa0589640 AT5G01340.1 24.306 288 191 8 90 357 18 298 3.08e-16 79.0
Msa0589640 AT5G46800.2 25.952 289 188 9 91 355 10 296 3.64e-15 75.9
Msa0589640 AT5G46800.2 28.497 193 123 4 89 267 108 299 7.24e-15 75.1
Msa0589640 AT5G46800.3 25.952 289 188 9 91 355 10 296 3.64e-15 75.9
Msa0589640 AT5G46800.3 28.497 193 123 4 89 267 108 299 7.24e-15 75.1
Msa0589640 AT5G46800.1 25.952 289 188 9 91 355 10 296 3.64e-15 75.9
Msa0589640 AT5G46800.1 28.497 193 123 4 89 267 108 299 7.24e-15 75.1
Msa0589640 AT2G26360.1 24.398 332 217 12 41 356 61 374 5.61e-15 76.3
Msa0589640 AT1G74240.1 23.355 304 190 9 89 355 36 333 5.02e-14 73.2
Msa0589640 AT4G24570.1 23.934 305 197 5 89 358 6 310 6.42e-14 72.4
Msa0589640 AT5G26200.1 25.379 264 159 8 138 363 79 342 1.11e-13 72.0
Msa0589640 AT5G26200.1 27.273 198 120 5 189 364 41 236 1.81e-13 71.2
Msa0589640 AT2G22500.1 24.172 302 196 7 89 357 6 307 1.57e-12 68.2
Msa0589640 AT1G72820.1 27.807 187 116 4 189 358 33 217 2.26e-12 68.2
Msa0589640 AT1G72820.2 27.807 187 116 4 189 358 33 217 2.26e-12 68.2
Msa0589640 AT2G35800.1 22.222 279 196 8 90 362 546 809 2.59e-12 68.9
Msa0589640 AT5G58970.2 23.377 231 163 4 89 305 16 246 6.04e-12 65.9
Msa0589640 AT1G79900.1 21.371 248 188 2 118 358 36 283 1.57e-11 65.1
Msa0589640 AT3G48850.1 25.824 182 122 5 189 358 67 247 3.43e-11 64.7

Find 120 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGATTTGTGCAACTTAT+TGG 0.147653 4_2:-12833372 Msa0589640:three_prime_UTR
AAGTTCACCATCATTGATTA+TGG 0.174350 4_2:+12833605 None:intergenic
GGTGGAGACGGTGGCGAATT+TGG 0.213603 4_2:+12837361 None:intergenic
TTCTTTCCCTATCATTGATA+TGG 0.215148 4_2:+12835394 None:intergenic
GTTAGAGGTGGTGGTTAAAT+TGG 0.220101 4_2:+12837457 None:intergenic
TTGTTCGTTGTGTCTGTCAT+AGG 0.236276 4_2:+12837506 None:intergenic
GTGGCACAAGATGGAGTTAT+TGG 0.246853 4_2:-12834062 Msa0589640:CDS
ACAGTATTAGATGCCATTTC+AGG 0.261132 4_2:-12834335 Msa0589640:intron
GTAGGACTCAAGTTTGTTCT+AGG 0.268017 4_2:-12833476 Msa0589640:three_prime_UTR
AGGGATAAGTTTGATGGTTT+TGG 0.270207 4_2:+12837526 None:intergenic
TACTGCGTGTGTTTGCTTAA+TGG 0.274617 4_2:+12837563 None:intergenic
ATGAACAAACATCTCCTATT+AGG 0.279014 4_2:-12833348 Msa0589640:three_prime_UTR
GGCCTTGGTCCTTCTCTTAT+CGG 0.280665 4_2:-12834643 Msa0589640:CDS
GAGAAACAATGCAGCATCTT+TGG 0.280947 4_2:+12837256 None:intergenic
GCATCTAATACTGTCTTATA+AGG 0.297227 4_2:+12834344 None:intergenic
CTTTGCTTATGAAATTTATA+AGG 0.302156 4_2:-12835215 Msa0589640:intron
TTAGAGGTGGTGGTTAAATT+GGG 0.348256 4_2:+12837458 None:intergenic
CAAAAGTTAACTGCGATATA+TGG 0.353568 4_2:+12834613 None:intergenic
CCAAAGGGATAAAACCTAAT+AGG 0.355225 4_2:+12833334 None:intergenic
TAAGAAGCTGAGCCGGCGTC+GGG 0.359061 4_2:+12837309 None:intergenic
GGGAAAGAAGAAGGCATTAG+AGG 0.360817 4_2:-12835380 Msa0589640:CDS
GCTTCTTTCTACTACGGCCT+TGG 0.361517 4_2:-12834658 Msa0589640:CDS
AAGAAGGCATTAGAGGTTAC+TGG 0.362804 4_2:-12835373 Msa0589640:CDS
TTTCTCGCTGGTGCTGTTGC+CGG 0.371610 4_2:-12837239 Msa0589640:CDS
AAAGCAATTCCCACAACTAA+TGG 0.376764 4_2:+12833547 None:intergenic
TTGCAGGCCATATCAATGAT+AGG 0.383329 4_2:-12835401 Msa0589640:intron
AAGAGCTGAACAGCACTATA+AGG 0.384778 4_2:+12835235 None:intergenic
GTCGGGGCGAATTCGTTGTT+CGG 0.386237 4_2:+12837326 None:intergenic
ATTCGTTGTTCGGTTTAGTT+TGG 0.394789 4_2:+12837336 None:intergenic
ATATCTCACTGCTAGCAAAT+TGG 0.395642 4_2:+12833522 None:intergenic
AGAAACAATGCAGCATCTTT+GGG 0.402197 4_2:+12837257 None:intergenic
TGTCATAGGGATAAGTTTGA+TGG 0.405847 4_2:+12837520 None:intergenic
GAAAGTGCTAAGAAGACAAT+TGG 0.415198 4_2:-12837052 Msa0589640:CDS
CAATGCCAACTAACCTGAAA+TGG 0.419802 4_2:+12834322 None:intergenic
CGTAATCTCAGGACATCTAA+AGG 0.428560 4_2:+12834825 None:intergenic
AATGATGAGCTCTCTGTTGT+CGG 0.431513 4_2:-12835094 Msa0589640:CDS
AAAAGTTGTTGAAATATAGT+TGG 0.437564 4_2:-12837644 Msa0589640:intron
ATTACAGCTAAAATACATTC+AGG 0.438813 4_2:+12833428 None:intergenic
AGTTCACCATCATTGATTAT+GGG 0.444924 4_2:+12833606 None:intergenic
CAAGAGCGACGACGGCGATC+GGG 0.448099 4_2:+12837282 None:intergenic
CCTTCTCACTAGCAGCAATC+AGG 0.448175 4_2:+12833675 None:intergenic
GGATAAAGAATAGAGCATTA+TGG 0.451407 4_2:+12833449 None:intergenic
CAGTTGTTGAAGAGGTGGTT+GGG 0.453871 4_2:+12837422 None:intergenic
TAAAATTATCCATTAGTTGT+GGG 0.456350 4_2:-12833556 Msa0589640:three_prime_UTR
GATCACAAGTACCTGCTGTT+TGG 0.457445 4_2:+12833999 None:intergenic
GGGTGAAACGACGTCGTTAG+AGG 0.465059 4_2:+12837442 None:intergenic
CAGACTCATGGAGTGCGGAT+TGG 0.468553 4_2:-12837079 Msa0589640:CDS
TTACGATTAGCTGTTGAACC+TGG 0.470496 4_2:-12834807 Msa0589640:CDS
GCAGTTGTTGAAGAGGTGGT+TGG 0.471890 4_2:+12837421 None:intergenic
ACAAGAGCGACGACGGCGAT+CGG 0.484450 4_2:+12837281 None:intergenic
TGTTCGTTGTGTCTGTCATA+GGG 0.497418 4_2:+12837507 None:intergenic
GTAATCTCAGGACATCTAAA+GGG 0.501170 4_2:+12834826 None:intergenic
AAATTTACAGAAAATATTCA+AGG 0.503522 4_2:-12835122 Msa0589640:intron
TTAAGAAGCTGAGCCGGCGT+CGG 0.508929 4_2:+12837308 None:intergenic
ATAAAATTATCCATTAGTTG+TGG 0.515167 4_2:-12833557 Msa0589640:three_prime_UTR
CGCCTTGAGCATGGTAAGAG+AGG 0.526243 4_2:-12834689 Msa0589640:CDS
TAATCAATGATGGTGAACTT+AGG 0.527251 4_2:-12833602 Msa0589640:three_prime_UTR
AACGACGTCGTTAGAGGTGG+TGG 0.529351 4_2:+12837448 None:intergenic
TAGAGGTGGTGGTTAAATTG+GGG 0.531755 4_2:+12837459 None:intergenic
GAAGACAATTGGTTTCATTG+AGG 0.535119 4_2:-12837041 Msa0589640:intron
GTTGTTCGGTTTAGTTTGGA+AGG 0.538720 4_2:+12837340 None:intergenic
TCGAATCAAACTTCTCATGC+AGG 0.539543 4_2:-12837183 Msa0589640:intron
AGAAGTTTGATTCGATCAAG+TGG 0.545093 4_2:+12837191 None:intergenic
GAAACGCCCATAATCAATGA+TGG 0.546128 4_2:-12833612 Msa0589640:exon
AAGCATTGGGCACAAATCCT+CGG 0.546969 4_2:+12834033 None:intergenic
ACAAGTACCTGCTGTTTGGT+AGG 0.550522 4_2:+12834003 None:intergenic
TCGATCAAGTGGAGCAGTGA+CGG 0.556672 4_2:+12837202 None:intergenic
CACACTTACATGCTATCCTC+TGG 0.561081 4_2:-12834402 Msa0589640:CDS
CAACAGCTAATCGTAATCTC+AGG 0.561207 4_2:+12834814 None:intergenic
GATGCTGCATTGTTTCTCGC+TGG 0.563132 4_2:-12837251 Msa0589640:CDS
GGCGTTTCTTTAGTTTGTTG+CGG 0.563544 4_2:+12833627 None:intergenic
TTTAGTTTGGAAGGTGGAGA+CGG 0.566249 4_2:+12837349 None:intergenic
TTTATAGGTCGCCTTGAGCA+TGG 0.566530 4_2:-12834698 Msa0589640:intron
CAACGAATTCGCCCCGACGC+CGG 0.566929 4_2:-12837321 Msa0589640:CDS
GATGTTACGCCACGTGGCGA+CGG 0.567513 4_2:+12837481 None:intergenic
TTGAGCATGGTAAGAGAGGA+AGG 0.567910 4_2:-12834685 Msa0589640:CDS
AATTTACAGAAAATATTCAA+GGG 0.571080 4_2:-12835121 Msa0589640:intron
GGTGGCGAATTTGGAAGGAA+TGG 0.574574 4_2:+12837370 None:intergenic
GGCAAGAGATGCTGAAAGCA+CGG 0.575833 4_2:+12834424 None:intergenic
TACGATTAGCTGTTGAACCT+GGG 0.579092 4_2:-12834806 Msa0589640:CDS
TGAAGGTGCAGTTGTTGAAG+AGG 0.579448 4_2:+12837414 None:intergenic
GTTGTCGGAAGACTTGCAGC+AGG 0.579618 4_2:-12835079 Msa0589640:CDS
TGCAGGCCATATCAATGATA+GGG 0.580409 4_2:-12835400 Msa0589640:intron
GAGACGGTGGCGAATTTGGA+AGG 0.581708 4_2:+12837365 None:intergenic
GGAGTTATTGGCTTGTACCG+AGG 0.582134 4_2:-12834050 Msa0589640:CDS
GAGCGACGACGGCGATCGGG+TGG 0.583645 4_2:+12837285 None:intergenic
ATTGGGGATGTTACGCCACG+TGG 0.585033 4_2:+12837475 None:intergenic
TTTGATTGATGCAGGTATTG+TGG 0.586365 4_2:-12834081 Msa0589640:intron
GCAGGTATTGTGGCACAAGA+TGG 0.586976 4_2:-12834071 Msa0589640:intron
GGGTGGTTAAGAAGCTGAGC+CGG 0.587213 4_2:+12837302 None:intergenic
AGGTGCAGTTGTTGAAGAGG+TGG 0.589863 4_2:+12837417 None:intergenic
GAATTTGGAAGGAATGGTGG+TGG 0.593957 4_2:+12837376 None:intergenic
GGCATTAGAGGTTACTGGAA+GGG 0.594350 4_2:-12835368 Msa0589640:CDS
CCTGATTGCTGCTAGTGAGA+AGG 0.594558 4_2:-12833675 Msa0589640:CDS
TAGCAGTGAGATATCTCAGA+AGG 0.596118 4_2:-12833513 Msa0589640:three_prime_UTR
GGAGTTTCAAACAATTGCAG+AGG 0.597624 4_2:-12833654 Msa0589640:CDS
GTTCGGTTTAGTTTGGAAGG+TGG 0.599225 4_2:+12837343 None:intergenic
TCTGTCATTGTTCGACACCC+AGG 0.599777 4_2:+12834789 None:intergenic
TCGAAGACAGATGCAACTGA+AGG 0.601278 4_2:-12834372 Msa0589640:CDS
TCAATGATAGGGAAAGAAGA+AGG 0.601397 4_2:-12835389 Msa0589640:CDS
TTCCTCTCTTACCATGCTCA+AGG 0.602242 4_2:+12834687 None:intergenic
TAGTGATGCTCACCTGTGGT+AGG 0.606895 4_2:+12835342 None:intergenic
GGAGCTATTCCGATAAGAGA+AGG 0.610783 4_2:+12834634 None:intergenic
GGCGAATTTGGAAGGAATGG+TGG 0.612416 4_2:+12837373 None:intergenic
AGCAGTGAGATATCTCAGAA+GGG 0.619251 4_2:-12833512 Msa0589640:three_prime_UTR
CGAAGACAGATGCAACTGAA+GGG 0.629981 4_2:-12834371 Msa0589640:CDS
TTTGGGAACAAGAGCGACGA+CGG 0.632313 4_2:+12837274 None:intergenic
CTGGAAGGGTAACCTACCAC+AGG 0.641606 4_2:-12835354 Msa0589640:intron
CAGAGGATAGCATGTAAGTG+TGG 0.643661 4_2:+12834403 None:intergenic
AGTTTGGAAGGTGGAGACGG+TGG 0.647095 4_2:+12837352 None:intergenic
AGGCATTAGAGGTTACTGGA+AGG 0.648807 4_2:-12835369 Msa0589640:CDS
GCAGTGAGATATCTCAGAAG+GGG 0.651708 4_2:-12833511 Msa0589640:three_prime_UTR
CGAACAAAACCGTCGCCACG+TGG 0.655549 4_2:-12837490 Msa0589640:CDS
TGAAACGACGTCGTTAGAGG+TGG 0.660799 4_2:+12837445 None:intergenic
TTCCGATAAGAGAAGGACCA+AGG 0.667681 4_2:+12834641 None:intergenic
AAGAAGCTGAGCCGGCGTCG+GGG 0.667870 4_2:+12837310 None:intergenic
TGTCTTCGAACTGTGTCCAG+AGG 0.670978 4_2:+12834386 None:intergenic
GGTTGCGTCGAGTAAAGTGA+AGG 0.718129 4_2:+12837397 None:intergenic
TGGGTGTCGAACAATGACAG+AGG 0.760125 4_2:-12834787 Msa0589640:intron
AAGAGATGCTGAAAGCACGG+CGG 0.775581 4_2:+12834427 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTAATATTATGAGTTTAA+TGG - chr4_2:12834283-12834302 Msa0589640:intron 15.0%
!!! AATAAATTGATTTTTGAGTA+GGG + chr4_2:12834910-12834929 None:intergenic 15.0%
!! AAATATAAAATCCTTAATCA+GGG + chr4_2:12835414-12835433 None:intergenic 15.0%
!! AAAATATAAAATCCTTAATC+AGG + chr4_2:12835415-12835434 None:intergenic 15.0%
!!! GGATTTTATATTTTTTTTGT+TGG - chr4_2:12835421-12835440 Msa0589640:intron 15.0%
!! AACATTACCTATAAATTTAT+TGG - chr4_2:12835536-12835555 Msa0589640:intron 15.0%
!! AAATTTACAGAAAATATTCA+AGG - chr4_2:12835856-12835875 Msa0589640:intron 15.0%
!! AATTTACAGAAAATATTCAA+GGG - chr4_2:12835857-12835876 Msa0589640:intron 15.0%
!!! TTATTTTTTTGCAAATTTAC+TGG + chr4_2:12837097-12837116 None:intergenic 15.0%
!! TAAATTTGCAAAAAAATAAG+TGG - chr4_2:12837098-12837117 Msa0589640:intron 15.0%
!!! ATGATATATTTAGTTGATAA+TGG - chr4_2:12837194-12837213 Msa0589640:CDS 15.0%
!! AAAAGTTGTTGAAATATAGT+TGG - chr4_2:12833334-12833353 Msa0589640:three_prime_UTR 20.0%
!! ATATGATATCATGTCAATTA+AGG + chr4_2:12834212-12834231 None:intergenic 20.0%
!! TAGTTGACATATTCATTTAA+TGG - chr4_2:12834340-12834359 Msa0589640:CDS 20.0%
!!! TAATGATTATTTTACACTGT+CGG - chr4_2:12834370-12834389 Msa0589640:CDS 20.0%
!!! TAAAAAGAACATTTTCATCA+TGG - chr4_2:12834661-12834680 Msa0589640:CDS 20.0%
!!! AAAAAGAACATTTTCATCAT+GGG - chr4_2:12834662-12834681 Msa0589640:CDS 20.0%
!!! CAATAAATTGATTTTTGAGT+AGG + chr4_2:12834911-12834930 None:intergenic 20.0%
!!! AATTGTTTTAGTTCTGTAAT+TGG - chr4_2:12835313-12835332 Msa0589640:intron 20.0%
!! AATATAAAATCCTTAATCAG+GGG + chr4_2:12835413-12835432 None:intergenic 20.0%
!!! TAATTTTTAGCGATATTAGA+TGG - chr4_2:12835487-12835506 Msa0589640:intron 20.0%
!! CTTTGCTTATGAAATTTATA+AGG - chr4_2:12835763-12835782 Msa0589640:intron 20.0%
!! AAATATTTGTAAACCATACA+AGG + chr4_2:12835981-12836000 None:intergenic 20.0%
!! CACTTTACAAAAAAAATTAC+TGG + chr4_2:12836062-12836081 None:intergenic 20.0%
!!! ATTAATATTTTGATTGATGC+AGG - chr4_2:12836889-12836908 Msa0589640:intron 20.0%
!! ATCAGCAAAATTAGAAATTA+AGG + chr4_2:12837049-12837068 None:intergenic 20.0%
!! ATAAAATTATCCATTAGTTG+TGG - chr4_2:12837421-12837440 Msa0589640:CDS 20.0%
!! TAAAATTATCCATTAGTTGT+GGG - chr4_2:12837422-12837441 Msa0589640:CDS 20.0%
! GTTACACATACATATAATTG+AGG - chr4_2:12834138-12834157 Msa0589640:intron 25.0%
! TTGACATGATATCATATCAT+TGG - chr4_2:12834215-12834234 Msa0589640:intron 25.0%
! TGAATATGTCAACTAATATG+TGG + chr4_2:12834335-12834354 None:intergenic 25.0%
!! GGAATTTGTTTATACCATTT+CGG - chr4_2:12834494-12834513 Msa0589640:intron 25.0%
!!! GTGTTCTAAAATGAATGTAA+TGG + chr4_2:12834572-12834591 None:intergenic 25.0%
! AATTGAAGACTTCATTTGAT+CGG + chr4_2:12834764-12834783 None:intergenic 25.0%
! TCTTGAGATAAAAATAGATG+TGG + chr4_2:12834968-12834987 None:intergenic 25.0%
! GAAAACTACATGAAAATTGA+TGG + chr4_2:12835371-12835390 None:intergenic 25.0%
! GTACTAAATGTGTAAGTTAA+TGG - chr4_2:12835443-12835462 Msa0589640:intron 25.0%
! AAATTATTGTCAAGGAGTTT+GGG + chr4_2:12835473-12835492 None:intergenic 25.0%
! AAAATTATTGTCAAGGAGTT+TGG + chr4_2:12835474-12835493 None:intergenic 25.0%
! ATCGCTAAAAATTATTGTCA+AGG + chr4_2:12835481-12835500 None:intergenic 25.0%
! GTAAGCTCCAATAAATTTAT+AGG + chr4_2:12835546-12835565 None:intergenic 25.0%
! AAAGACTACTCAACATTATA+AGG + chr4_2:12835681-12835700 None:intergenic 25.0%
!!! TTGTTTTAGTGTTTCTTTTC+AGG - chr4_2:12835706-12835725 Msa0589640:intron 25.0%
! AATTCTTGAAAGATAGTTAG+CGG + chr4_2:12835793-12835812 None:intergenic 25.0%
! ATTAAAACGGGAAAGATATA+AGG + chr4_2:12836244-12836263 None:intergenic 25.0%
! ACAATTCTGTTCATTAAAAC+GGG + chr4_2:12836256-12836275 None:intergenic 25.0%
! CACAATTCTGTTCATTAAAA+CGG + chr4_2:12836257-12836276 None:intergenic 25.0%
!!! CTTTATCTTTTTAAGTGCAT+TGG - chr4_2:12836419-12836438 Msa0589640:intron 25.0%
! TAAGACAAATTAACCTTGTA+TGG - chr4_2:12836465-12836484 Msa0589640:intron 25.0%
! AAGACAAATTAACCTTGTAT+GGG - chr4_2:12836466-12836485 Msa0589640:intron 25.0%
! TTATTGCTATAACAGCTAAA+AGG + chr4_2:12836786-12836805 None:intergenic 25.0%
! AGCAAAATTAGAAATTAAGG+AGG + chr4_2:12837046-12837065 None:intergenic 25.0%
! ATTACAGCTAAAATACATTC+AGG + chr4_2:12837553-12837572 None:intergenic 25.0%
!! AAATGATTTGTGCAACTTAT+TGG - chr4_2:12837606-12837625 Msa0589640:intron 25.0%
GATAATTTGATATACACCGT+CGG - chr4_2:12833982-12834001 Msa0589640:intron 30.0%
TTCAACCACATTAAATGATG+TGG + chr4_2:12834169-12834188 None:intergenic 30.0%
GTTTAATGGAAGTAAACTGA+CGG - chr4_2:12834297-12834316 Msa0589640:intron 30.0%
! TCAAGTAACGCATTTTGAAT+AGG + chr4_2:12834525-12834544 None:intergenic 30.0%
! CTCTTTCTTTTCTATTACTC+AGG + chr4_2:12834833-12834852 None:intergenic 30.0%
CTAAAACAATTACTAACCAG+AGG + chr4_2:12835304-12835323 None:intergenic 30.0%
AATTATTGTCAAGGAGTTTG+GGG + chr4_2:12835472-12835491 None:intergenic 30.0%
! TAGCGATATTAGATGGTTTT+TGG - chr4_2:12835494-12835513 Msa0589640:intron 30.0%
TTCTTTCCCTATCATTGATA+TGG + chr4_2:12835587-12835606 None:intergenic 30.0%
!!! TGTTTCTTTTCAGGTGATTA+GGG - chr4_2:12835715-12835734 Msa0589640:intron 30.0%
TTTGTAAACCATACAAGGAT+AGG + chr4_2:12835976-12835995 None:intergenic 30.0%
AGTAAGATACATTGACATCA+AGG + chr4_2:12836106-12836125 None:intergenic 30.0%
!! CGTTTTAATGAACAGAATTG+TGG - chr4_2:12836255-12836274 Msa0589640:intron 30.0%
! AACAGAATTGTGGCTTTTAT+AGG - chr4_2:12836265-12836284 Msa0589640:intron 30.0%
CAAAAGTTAACTGCGATATA+TGG + chr4_2:12836368-12836387 None:intergenic 30.0%
!!! TTTTTAAGTGCATTGGTGTT+TGG - chr4_2:12836426-12836445 Msa0589640:intron 30.0%
TTTAGTCTATTACCCATACA+AGG + chr4_2:12836481-12836500 None:intergenic 30.0%
! GTTCTCTTTTGATATTTCTC+TGG + chr4_2:12836514-12836533 None:intergenic 30.0%
GCATCTAATACTGTCTTATA+AGG + chr4_2:12836637-12836656 None:intergenic 30.0%
GCACAGAAAATGATTCTAAA+AGG + chr4_2:12836692-12836711 None:intergenic 30.0%
TATATGTAAGCACACATTGA+TGG - chr4_2:12836820-12836839 Msa0589640:intron 30.0%
!!! GGTAGGTTTTTTAAAGCATT+GGG + chr4_2:12836961-12836980 None:intergenic 30.0%
!!! TGGTAGGTTTTTTAAAGCAT+TGG + chr4_2:12836962-12836981 None:intergenic 30.0%
AATTAGAAATTAAGGAGGCA+TGG + chr4_2:12837041-12837060 None:intergenic 30.0%
GGATAAAATCCTGTTGAAAT+CGG - chr4_2:12837119-12837138 Msa0589640:intron 30.0%
! AACAAAAAACCGATTTCAAC+AGG + chr4_2:12837131-12837150 None:intergenic 30.0%
TTGTGTGTTCTTAGTAAGTT+GGG + chr4_2:12837167-12837186 None:intergenic 30.0%
! AGTTCACCATCATTGATTAT+GGG + chr4_2:12837375-12837394 None:intergenic 30.0%
! AAGTTCACCATCATTGATTA+TGG + chr4_2:12837376-12837395 None:intergenic 30.0%
! TAATCAATGATGGTGAACTT+AGG - chr4_2:12837376-12837395 Msa0589640:CDS 30.0%
! GGATAAAGAATAGAGCATTA+TGG + chr4_2:12837532-12837551 None:intergenic 30.0%
ATGAACAAACATCTCCTATT+AGG - chr4_2:12837630-12837649 Msa0589640:intron 30.0%
!! CTATTAGGTTTTATCCCTTT+GGG - chr4_2:12837645-12837664 Msa0589640:five_prime_UTR 30.0%
!! AGGGATAAGTTTGATGGTTT+TGG + chr4_2:12833455-12833474 None:intergenic 35.0%
TGTCATAGGGATAAGTTTGA+TGG + chr4_2:12833461-12833480 None:intergenic 35.0%
! TTAGAGGTGGTGGTTAAATT+GGG + chr4_2:12833523-12833542 None:intergenic 35.0%
ATTCGTTGTTCGGTTTAGTT+TGG + chr4_2:12833645-12833664 None:intergenic 35.0%
AGAAACAATGCAGCATCTTT+GGG + chr4_2:12833724-12833743 None:intergenic 35.0%
! AGAAGTTTGATTCGATCAAG+TGG + chr4_2:12833790-12833809 None:intergenic 35.0%
!!! TGTAGTTTTTTGCAGACTCA+TGG - chr4_2:12833887-12833906 Msa0589640:intron 35.0%
!! GAAAGTGCTAAGAAGACAAT+TGG - chr4_2:12833926-12833945 Msa0589640:intron 35.0%
! GAAGACAATTGGTTTCATTG+AGG - chr4_2:12833937-12833956 Msa0589640:intron 35.0%
TGATGCCACATCATTTAATG+TGG - chr4_2:12834161-12834180 Msa0589640:intron 35.0%
!! GTTTCTCGTTGGTAGTTAAT+TGG - chr4_2:12834467-12834486 Msa0589640:CDS 35.0%
TTGAATAGGAAACACCGAAA+TGG + chr4_2:12834511-12834530 None:intergenic 35.0%
TGGTTACATGATGTCAAAGA+TGG + chr4_2:12834705-12834724 None:intergenic 35.0%
!! AAAATAGATGTGGCTCTAGA+AGG + chr4_2:12834958-12834977 None:intergenic 35.0%
TTGAGTCAGCTATTATCTGA+CGG - chr4_2:12835002-12835021 Msa0589640:intron 35.0%
TAATCCAGTTTGCAGTCTAA+AGG - chr4_2:12835336-12835355 Msa0589640:intron 35.0%
AATCCAGTTTGCAGTCTAAA+GGG - chr4_2:12835337-12835356 Msa0589640:intron 35.0%
GTAATTAACTCCCCTGATTA+AGG - chr4_2:12835400-12835419 Msa0589640:intron 35.0%
TCAATGATAGGGAAAGAAGA+AGG - chr4_2:12835589-12835608 Msa0589640:intron 35.0%
!!! GTGTTTCTTTTCAGGTGATT+AGG - chr4_2:12835714-12835733 Msa0589640:intron 35.0%
GTACCAAACATTACATGTTC+AGG - chr4_2:12835817-12835836 Msa0589640:intron 35.0%
ACACCTGAACATGTAATGTT+TGG + chr4_2:12835823-12835842 None:intergenic 35.0%
TTATGAGACCTATCCTTGTA+TGG - chr4_2:12835965-12835984 Msa0589640:intron 35.0%
GTAATCTCAGGACATCTAAA+GGG + chr4_2:12836155-12836174 None:intergenic 35.0%
! AACAATGACAGAGGTATATG+TGG - chr4_2:12836200-12836219 Msa0589640:intron 35.0%
! ACAATGACAGAGGTATATGT+GGG - chr4_2:12836201-12836220 Msa0589640:intron 35.0%
! GGTTTTGCTTCTTTCTACTA+CGG - chr4_2:12836314-12836333 Msa0589640:intron 35.0%
! ACAGTATTAGATGCCATTTC+AGG - chr4_2:12836643-12836662 Msa0589640:intron 35.0%
! GCAAGTTTTGCACAGATAAA+GGG + chr4_2:12836757-12836776 None:intergenic 35.0%
! TGCAAGTTTTGCACAGATAA+AGG + chr4_2:12836758-12836777 None:intergenic 35.0%
! TTTGATTGATGCAGGTATTG+TGG - chr4_2:12836897-12836916 Msa0589640:intron 35.0%
TAAAAAACCTACCAAACAGC+AGG - chr4_2:12836968-12836987 Msa0589640:intron 35.0%
GTTGTGTGTTCTTAGTAAGT+TGG + chr4_2:12837168-12837187 None:intergenic 35.0%
!! ACTTTGAAGCAGATTGTGAT+TGG - chr4_2:12837228-12837247 Msa0589640:CDS 35.0%
AAAGCAATTCCCACAACTAA+TGG + chr4_2:12837434-12837453 None:intergenic 35.0%
ATATCTCACTGCTAGCAAAT+TGG + chr4_2:12837459-12837478 None:intergenic 35.0%
! AAGGGGACTGTAAATTTTGT+AGG - chr4_2:12837484-12837503 Msa0589640:CDS 35.0%
CCAAAGGGATAAAACCTAAT+AGG + chr4_2:12837647-12837666 None:intergenic 35.0%
!! CCTATTAGGTTTTATCCCTT+TGG - chr4_2:12837644-12837663 Msa0589640:intron 35.0%
TACTGCGTGTGTTTGCTTAA+TGG + chr4_2:12833418-12833437 None:intergenic 40.0%
TGTTCGTTGTGTCTGTCATA+GGG + chr4_2:12833474-12833493 None:intergenic 40.0%
TTGTTCGTTGTGTCTGTCAT+AGG + chr4_2:12833475-12833494 None:intergenic 40.0%
! TAGAGGTGGTGGTTAAATTG+GGG + chr4_2:12833522-12833541 None:intergenic 40.0%
! GTTAGAGGTGGTGGTTAAAT+TGG + chr4_2:12833524-12833543 None:intergenic 40.0%
!! TTTAGTTTGGAAGGTGGAGA+CGG + chr4_2:12833632-12833651 None:intergenic 40.0%
!! GTTGTTCGGTTTAGTTTGGA+AGG + chr4_2:12833641-12833660 None:intergenic 40.0%
GAGAAACAATGCAGCATCTT+TGG + chr4_2:12833725-12833744 None:intergenic 40.0%
TCGAATCAAACTTCTCATGC+AGG - chr4_2:12833795-12833814 Msa0589640:intron 40.0%
! GTGTAACTCTTTTACACCGA+CGG + chr4_2:12834001-12834020 None:intergenic 40.0%
! GCTTAACAACTGTTTCTCGT+TGG - chr4_2:12834456-12834475 Msa0589640:CDS 40.0%
!! CGTTGGTAGTTAATTGGTTG+TGG - chr4_2:12834473-12834492 Msa0589640:CDS 40.0%
AGAGCTATTTGAGGAACTCT+TGG + chr4_2:12834725-12834744 None:intergenic 40.0%
AAGAGTTCCTCAAATAGCTC+TGG - chr4_2:12834724-12834743 Msa0589640:intron 40.0%
ACAAGTTCCAGAGCTATTTG+AGG + chr4_2:12834734-12834753 None:intergenic 40.0%
ACTGATATGTCCTATGCTAG+AGG - chr4_2:12834804-12834823 Msa0589640:CDS 40.0%
AATGCAAGTTGTGCATGAGA+TGG + chr4_2:12835251-12835270 None:intergenic 40.0%
ATACCCTTTAGACTGCAAAC+TGG + chr4_2:12835343-12835362 None:intergenic 40.0%
CTTACGTTGACAAGATTTGC+AGG - chr4_2:12835561-12835580 Msa0589640:intron 40.0%
TTGCAGGCCATATCAATGAT+AGG - chr4_2:12835577-12835596 Msa0589640:intron 40.0%
TGCAGGCCATATCAATGATA+GGG - chr4_2:12835578-12835597 Msa0589640:intron 40.0%
!! AAGAAGGCATTAGAGGTTAC+TGG - chr4_2:12835605-12835624 Msa0589640:intron 40.0%
AAGAGCTGAACAGCACTATA+AGG + chr4_2:12835746-12835765 None:intergenic 40.0%
AATGATGAGCTCTCTGTTGT+CGG - chr4_2:12835884-12835903 Msa0589640:intron 40.0%
CGTAATCTCAGGACATCTAA+AGG + chr4_2:12836156-12836175 None:intergenic 40.0%
CAACAGCTAATCGTAATCTC+AGG + chr4_2:12836167-12836186 None:intergenic 40.0%
! TTACGATTAGCTGTTGAACC+TGG - chr4_2:12836171-12836190 Msa0589640:intron 40.0%
! TACGATTAGCTGTTGAACCT+GGG - chr4_2:12836172-12836191 Msa0589640:intron 40.0%
!! AGATGCCATTTCAGGTTAGT+TGG - chr4_2:12836651-12836670 Msa0589640:intron 40.0%
CAATGCCAACTAACCTGAAA+TGG + chr4_2:12836659-12836678 None:intergenic 40.0%
CCTAATGCTGCAAAAACAGA+AGG + chr4_2:12837257-12837276 None:intergenic 40.0%
!!! CCTTCTGTTTTTGCAGCATT+AGG - chr4_2:12837254-12837273 Msa0589640:CDS 40.0%
GGAGTTTCAAACAATTGCAG+AGG - chr4_2:12837324-12837343 Msa0589640:CDS 40.0%
!! GGCGTTTCTTTAGTTTGTTG+CGG + chr4_2:12837354-12837373 None:intergenic 40.0%
GAAACGCCCATAATCAATGA+TGG - chr4_2:12837366-12837385 Msa0589640:CDS 40.0%
TAGCAGTGAGATATCTCAGA+AGG - chr4_2:12837465-12837484 Msa0589640:CDS 40.0%
AGCAGTGAGATATCTCAGAA+GGG - chr4_2:12837466-12837485 Msa0589640:CDS 40.0%
! GTAGGACTCAAGTTTGTTCT+AGG - chr4_2:12837502-12837521 Msa0589640:CDS 40.0%
CAGTTGTTGAAGAGGTGGTT+GGG + chr4_2:12833559-12833578 None:intergenic 45.0%
! TGAAGGTGCAGTTGTTGAAG+AGG + chr4_2:12833567-12833586 None:intergenic 45.0%
GAATTTGGAAGGAATGGTGG+TGG + chr4_2:12833605-12833624 None:intergenic 45.0%
!! GTTCGGTTTAGTTTGGAAGG+TGG + chr4_2:12833638-12833657 None:intergenic 45.0%
! TTTTGCAGACTCATGGAGTG+CGG - chr4_2:12833894-12833913 Msa0589640:intron 45.0%
CTTCACGTATAACCCAAGCA+CGG + chr4_2:12834401-12834420 None:intergenic 45.0%
ACTCAGGAATCCTCTAGCAT+AGG + chr4_2:12834817-12834836 None:intergenic 45.0%
TCTAGAAGGAGGGGAACATT+TGG + chr4_2:12834944-12834963 None:intergenic 45.0%
!! TAGATGTGGCTCTAGAAGGA+GGG + chr4_2:12834954-12834973 None:intergenic 45.0%
!! ATAGATGTGGCTCTAGAAGG+AGG + chr4_2:12834955-12834974 None:intergenic 45.0%
CTATATGTACGACTTGCCTC+TGG - chr4_2:12835285-12835304 Msa0589640:intron 45.0%
TATTGTCAAGGAGTTTGGGG+AGG + chr4_2:12835469-12835488 None:intergenic 45.0%
!! GGGAAAGAAGAAGGCATTAG+AGG - chr4_2:12835598-12835617 Msa0589640:intron 45.0%
AGGCATTAGAGGTTACTGGA+AGG - chr4_2:12835609-12835628 Msa0589640:intron 45.0%
GGCATTAGAGGTTACTGGAA+GGG - chr4_2:12835610-12835629 Msa0589640:intron 45.0%
TATCTAGTGATGCTCACCTG+TGG + chr4_2:12835643-12835662 None:intergenic 45.0%
TTTATAGGTCGCCTTGAGCA+TGG - chr4_2:12836280-12836299 Msa0589640:intron 45.0%
TTCCTCTCTTACCATGCTCA+AGG + chr4_2:12836294-12836313 None:intergenic 45.0%
TTGAGCATGGTAAGAGAGGA+AGG - chr4_2:12836293-12836312 Msa0589640:intron 45.0%
TTCCGATAAGAGAAGGACCA+AGG + chr4_2:12836340-12836359 None:intergenic 45.0%
GGAGCTATTCCGATAAGAGA+AGG + chr4_2:12836347-12836366 None:intergenic 45.0%
CAGAGGATAGCATGTAAGTG+TGG + chr4_2:12836578-12836597 None:intergenic 45.0%
CACACTTACATGCTATCCTC+TGG - chr4_2:12836576-12836595 Msa0589640:intron 45.0%
TCGAAGACAGATGCAACTGA+AGG - chr4_2:12836606-12836625 Msa0589640:intron 45.0%
CGAAGACAGATGCAACTGAA+GGG - chr4_2:12836607-12836626 Msa0589640:intron 45.0%
GTGGCACAAGATGGAGTTAT+TGG - chr4_2:12836916-12836935 Msa0589640:intron 45.0%
!! AAGCATTGGGCACAAATCCT+CGG + chr4_2:12836948-12836967 None:intergenic 45.0%
! ACAAGTACCTGCTGTTTGGT+AGG + chr4_2:12836978-12836997 None:intergenic 45.0%
GATCACAAGTACCTGCTGTT+TGG + chr4_2:12836982-12837001 None:intergenic 45.0%
GCAGTGAGATATCTCAGAAG+GGG - chr4_2:12837467-12837486 Msa0589640:CDS 45.0%
!!! ATTGATTTTTTTTTTTTTTA+AGG + chr4_2:12833379-12833398 None:intergenic 5.0%
!! CTTATATAAAAAAAAATATA+AGG - chr4_2:12835143-12835162 Msa0589640:intron 5.0%
TGAAACGACGTCGTTAGAGG+TGG + chr4_2:12833536-12833555 None:intergenic 50.0%
GCAGTTGTTGAAGAGGTGGT+TGG + chr4_2:12833560-12833579 None:intergenic 50.0%
AGGTGCAGTTGTTGAAGAGG+TGG + chr4_2:12833564-12833583 None:intergenic 50.0%
GGTTGCGTCGAGTAAAGTGA+AGG + chr4_2:12833584-12833603 None:intergenic 50.0%
GGCGAATTTGGAAGGAATGG+TGG + chr4_2:12833608-12833627 None:intergenic 50.0%
GGTGGCGAATTTGGAAGGAA+TGG + chr4_2:12833611-12833630 None:intergenic 50.0%
TTTGGGAACAAGAGCGACGA+CGG + chr4_2:12833707-12833726 None:intergenic 50.0%
! GATGCTGCATTGTTTCTCGC+TGG - chr4_2:12833727-12833746 Msa0589640:intron 50.0%
TCGATCAAGTGGAGCAGTGA+CGG + chr4_2:12833779-12833798 None:intergenic 50.0%
AGTAGCTGCAGAGCTTGCAA+AGG + chr4_2:12834787-12834806 None:intergenic 50.0%
CTGCCGATGGAATTGCGAAT+AGG - chr4_2:12834884-12834903 Msa0589640:intron 50.0%
GGGCCTATTCGCAATTCCAT+CGG + chr4_2:12834890-12834909 None:intergenic 50.0%
!! AGATGTGGCTCTAGAAGGAG+GGG + chr4_2:12834953-12834972 None:intergenic 50.0%
TAGTGATGCTCACCTGTGGT+AGG + chr4_2:12835639-12835658 None:intergenic 50.0%
TCTGTCATTGTTCGACACCC+AGG + chr4_2:12836192-12836211 None:intergenic 50.0%
!! TGGGTGTCGAACAATGACAG+AGG - chr4_2:12836191-12836210 Msa0589640:intron 50.0%
GCTTCTTTCTACTACGGCCT+TGG - chr4_2:12836320-12836339 Msa0589640:intron 50.0%
GGCCTTGGTCCTTCTCTTAT+CGG - chr4_2:12836335-12836354 Msa0589640:intron 50.0%
! AAGAGATGCTGAAAGCACGG+CGG + chr4_2:12836554-12836573 None:intergenic 50.0%
GGCAAGAGATGCTGAAAGCA+CGG + chr4_2:12836557-12836576 None:intergenic 50.0%
TGTCTTCGAACTGTGTCCAG+AGG + chr4_2:12836595-12836614 None:intergenic 50.0%
! GCAGGTATTGTGGCACAAGA+TGG - chr4_2:12836907-12836926 Msa0589640:intron 50.0%
!! GGAGTTATTGGCTTGTACCG+AGG - chr4_2:12836928-12836947 Msa0589640:intron 50.0%
CCTTCTCACTAGCAGCAATC+AGG + chr4_2:12837306-12837325 None:intergenic 50.0%
CCTGATTGCTGCTAGTGAGA+AGG - chr4_2:12837303-12837322 Msa0589640:CDS 50.0%
ATTGGGGATGTTACGCCACG+TGG + chr4_2:12833506-12833525 None:intergenic 55.0%
AACGACGTCGTTAGAGGTGG+TGG + chr4_2:12833533-12833552 None:intergenic 55.0%
GGGTGAAACGACGTCGTTAG+AGG + chr4_2:12833539-12833558 None:intergenic 55.0%
GAGACGGTGGCGAATTTGGA+AGG + chr4_2:12833616-12833635 None:intergenic 55.0%
AGTTTGGAAGGTGGAGACGG+TGG + chr4_2:12833629-12833648 None:intergenic 55.0%
GTCGGGGCGAATTCGTTGTT+CGG + chr4_2:12833655-12833674 None:intergenic 55.0%
TTAAGAAGCTGAGCCGGCGT+CGG + chr4_2:12833673-12833692 None:intergenic 55.0%
GGGTGGTTAAGAAGCTGAGC+CGG + chr4_2:12833679-12833698 None:intergenic 55.0%
!! TTTCTCGCTGGTGCTGTTGC+CGG - chr4_2:12833739-12833758 Msa0589640:intron 55.0%
!! GACGGTTTTAGCAGCAGCTC+CGG + chr4_2:12833761-12833780 None:intergenic 55.0%
CAGACTCATGGAGTGCGGAT+TGG - chr4_2:12833899-12833918 Msa0589640:intron 55.0%
! ACTGTCGGTTATGCCGTGCT+TGG - chr4_2:12834385-12834404 Msa0589640:CDS 55.0%
! CTGTCGGTTATGCCGTGCTT+GGG - chr4_2:12834386-12834405 Msa0589640:CDS 55.0%
CTGGAAGGGTAACCTACCAC+AGG - chr4_2:12835624-12835643 Msa0589640:intron 55.0%
GTTGTCGGAAGACTTGCAGC+AGG - chr4_2:12835899-12835918 Msa0589640:intron 55.0%
! CTTGCAGCAGGTGCTTTTGC+AGG - chr4_2:12835911-12835930 Msa0589640:intron 55.0%
CGCCTTGAGCATGGTAAGAG+AGG - chr4_2:12836289-12836308 Msa0589640:intron 55.0%
CGAACAAAACCGTCGCCACG+TGG - chr4_2:12833488-12833507 Msa0589640:three_prime_UTR 60.0%
GATGTTACGCCACGTGGCGA+CGG + chr4_2:12833500-12833519 None:intergenic 60.0%
GGTGGAGACGGTGGCGAATT+TGG + chr4_2:12833620-12833639 None:intergenic 60.0%
TAAGAAGCTGAGCCGGCGTC+GGG + chr4_2:12833672-12833691 None:intergenic 60.0%
ACAAGAGCGACGACGGCGAT+CGG + chr4_2:12833700-12833719 None:intergenic 60.0%
CTCAGAGCATGCTCTGCCGA+TGG - chr4_2:12834871-12834890 Msa0589640:intron 60.0%
CAACGAATTCGCCCCGACGC+CGG - chr4_2:12833657-12833676 Msa0589640:CDS 65.0%
AAGAAGCTGAGCCGGCGTCG+GGG + chr4_2:12833671-12833690 None:intergenic 65.0%
CAAGAGCGACGACGGCGATC+GGG + chr4_2:12833699-12833718 None:intergenic 65.0%
GAGCGACGACGGCGATCGGG+TGG + chr4_2:12833696-12833715 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 12833330 12837670 12833330 ID=Msa0589640;Name=Msa0589640
chr4_2 mRNA 12833330 12837670 12833330 ID=Msa0589640-mRNA-1;Parent=Msa0589640;Name=Msa0589640-mRNA-1;_AED=0.17;_eAED=0.17;_QI=115|1|1|1|0.9|0.81|11|292|380
chr4_2 exon 12833330 12833730 12833330 ID=Msa0589640-mRNA-1:exon:3758;Parent=Msa0589640-mRNA-1
chr4_2 exon 12834011 12834089 12834011 ID=Msa0589640-mRNA-1:exon:3757;Parent=Msa0589640-mRNA-1
chr4_2 exon 12834336 12834505 12834336 ID=Msa0589640-mRNA-1:exon:3756;Parent=Msa0589640-mRNA-1
chr4_2 exon 12834601 12834713 12834601 ID=Msa0589640-mRNA-1:exon:3755;Parent=Msa0589640-mRNA-1
chr4_2 exon 12834788 12834856 12834788 ID=Msa0589640-mRNA-1:exon:3754;Parent=Msa0589640-mRNA-1
chr4_2 exon 12835051 12835134 12835051 ID=Msa0589640-mRNA-1:exon:3753;Parent=Msa0589640-mRNA-1
chr4_2 exon 12835216 12835272 12835216 ID=Msa0589640-mRNA-1:exon:3752;Parent=Msa0589640-mRNA-1
chr4_2 exon 12835355 12835417 12835355 ID=Msa0589640-mRNA-1:exon:3751;Parent=Msa0589640-mRNA-1
chr4_2 exon 12837042 12837098 12837042 ID=Msa0589640-mRNA-1:exon:3750;Parent=Msa0589640-mRNA-1
chr4_2 exon 12837184 12837614 12837184 ID=Msa0589640-mRNA-1:exon:3749;Parent=Msa0589640-mRNA-1
chr4_2 exon 12837645 12837670 12837645 ID=Msa0589640-mRNA-1:exon:3748;Parent=Msa0589640-mRNA-1
chr4_2 five_prime_UTR 12837645 12837670 12837645 ID=Msa0589640-mRNA-1:five_prime_utr;Parent=Msa0589640-mRNA-1
chr4_2 five_prime_UTR 12837526 12837614 12837526 ID=Msa0589640-mRNA-1:five_prime_utr;Parent=Msa0589640-mRNA-1
chr4_2 CDS 12837184 12837525 12837184 ID=Msa0589640-mRNA-1:cds;Parent=Msa0589640-mRNA-1
chr4_2 CDS 12837042 12837098 12837042 ID=Msa0589640-mRNA-1:cds;Parent=Msa0589640-mRNA-1
chr4_2 CDS 12835355 12835417 12835355 ID=Msa0589640-mRNA-1:cds;Parent=Msa0589640-mRNA-1
chr4_2 CDS 12835216 12835272 12835216 ID=Msa0589640-mRNA-1:cds;Parent=Msa0589640-mRNA-1
chr4_2 CDS 12835051 12835134 12835051 ID=Msa0589640-mRNA-1:cds;Parent=Msa0589640-mRNA-1
chr4_2 CDS 12834788 12834856 12834788 ID=Msa0589640-mRNA-1:cds;Parent=Msa0589640-mRNA-1
chr4_2 CDS 12834601 12834713 12834601 ID=Msa0589640-mRNA-1:cds;Parent=Msa0589640-mRNA-1
chr4_2 CDS 12834336 12834505 12834336 ID=Msa0589640-mRNA-1:cds;Parent=Msa0589640-mRNA-1
chr4_2 CDS 12834011 12834089 12834011 ID=Msa0589640-mRNA-1:cds;Parent=Msa0589640-mRNA-1
chr4_2 CDS 12833622 12833730 12833622 ID=Msa0589640-mRNA-1:cds;Parent=Msa0589640-mRNA-1
chr4_2 three_prime_UTR 12833330 12833621 12833330 ID=Msa0589640-mRNA-1:three_prime_utr;Parent=Msa0589640-mRNA-1
Gene Sequence

>Msa0589640

ATGACAGACACAACGAACAAAACCGTCGCCACGTGGCGTAACATCCCCAATTTAACCACCACCTCTAACGACGTCGTTTCACCCAACCACCTCTTCAACAACTGCACCTTCACTTTACTCGACGCAACCACCACCATTCCTTCCAAATTCGCCACCGTCTCCACCTTCCAAACTAAACCGAACAACGAATTCGCCCCGACGCCGGCTCAGCTTCTTAACCACCCGATCGCCGTCGTCGCTCTTGTTCCCAAAGATGCTGCATTGTTTCTCGCTGGTGCTGTTGCCGGAGCTGCTGCTAAAACCGTCACTGCTCCACTTGATCGAATCAAACTTCTCATGCAGACTCATGGAGTGCGGATTGGACAAGAAAGTGCTAAGAAGACAATTGGTTTCATTGAGGCCATATCAATGATAGGGAAAGAAGAAGGCATTAGAGGTTACTGGAAGGGTAACCTACCACAGGTGATTAGGGTTATACCTTATAGTGCTGTTCAGCTCTTTGCTTATGAAATTTATAAGAAAATATTCAAGGGAAAAAATGATGAGCTCTCTGTTGTCGGAAGACTTGCAGCAGGTGCTTTTGCAGGCATGACATCAACTTTTATAACTTACCCTTTAGATGTCCTGAGATTACGATTAGCTGTTGAACCTGGGTGTCGAACAATGACAGAGGTCGCCTTGAGCATGGTAAGAGAGGAAGGTTTTGCTTCTTTCTACTACGGCCTTGGTCCTTCTCTTATCGGAATAGCTCCATATATCGCAGTTAACTTTTGTGTTTTTGACTTACTAAAGAAGTCGTTGCCAGAGAAATATCAAAAGAGAACTGAAACATCTCTAGTCACCGCCGTGCTTTCAGCATCTCTTGCCACACTTACATGCTATCCTCTGGACACAGTTCGAAGACAGATGCAACTGAAGGGTACACCTTATAAGACAGTATTAGATGCCATTTCAGGTATTGTGGCACAAGATGGAGTTATTGGCTTGTACCGAGGATTTGTGCCCAATGCTTTAAAAAACCTACCAAACAGCAGCATTAGGCTTGCAACATATGACATTGTCAAACGCCTGATTGCTGCTAGTGAGAAGGAGTTTCAAACAATTGCAGAGGAAAACCGCAACAAACTAAAGAAACGCCCATAA
Protein sequence

>Msa0589640

MTDTTNKTVATWRNIPNLTTTSNDVVSPNHLFNNCTFTLLDATTTIPSKFATVSTFQTKPNNEFAPTPAQLLNHPIAVVALVPKDAALFLAGAVAGAAAKTVTAPLDRIKLLMQTHGVRIGQESAKKTIGFIEAISMIGKEEGIRGYWKGNLPQVIRVIPYSAVQLFAYEIYKKIFKGKNDELSVVGRLAAGAFAGMTSTFITYPLDVLRLRLAVEPGCRTMTEVALSMVREEGFASFYYGLGPSLIGIAPYIAVNFCVFDLLKKSLPEKYQKRTETSLVTAVLSASLATLTCYPLDTVRRQMQLKGTPYKTVLDAISGIVAQDGVIGLYRGFVPNALKNLPNSSIRLATYDIVKRLIAASEKEFQTIAEENRNKLKKRP*