AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0001460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0001460 MtrunA17_Chr3g0145781 91.176 544 23 5 1 528 3 537 0.0 998
Msa0001460 MtrunA17_Chr6g0466611 32.850 414 237 14 123 522 142 528 2.68e-52 189
Msa0001460 MtrunA17_Chr3g0107851 30.326 399 247 11 101 495 101 472 3.08e-42 159
Msa0001460 MtrunA17_Chr4g0062301 28.382 377 225 10 146 511 120 462 7.12e-41 155
Msa0001460 MtrunA17_Chr2g0330401 27.806 392 252 12 121 510 94 456 1.27e-39 150
Msa0001460 MtrunA17_Chr2g0307471 28.784 403 233 15 116 503 84 447 4.86e-39 149
Msa0001460 MtrunA17_Chr4g0063551 30.287 383 225 12 144 510 161 517 3.89e-38 148
Msa0001460 MtrunA17_Chr7g0270131 26.972 393 255 11 121 510 230 593 5.64e-36 142
Msa0001460 MtrunA17_Chr3g0098121 28.315 445 268 14 72 512 117 514 2.23e-35 140
Msa0001460 MtrunA17_Chr2g0300291 29.584 409 246 12 112 512 130 504 2.28e-34 137
Msa0001460 MtrunA17_Chr1g0193491 28.158 380 240 11 127 502 118 468 4.88e-34 136
Msa0001460 MtrunA17_Chr7g0271121 28.610 374 233 11 133 502 116 459 6.19e-34 136
Msa0001460 MtrunA17_Chr1g0157691 26.753 385 245 11 121 496 145 501 6.22e-34 135
Msa0001460 MtrunA17_Chr6g0449911 26.649 379 246 10 121 497 163 511 7.42e-34 136
Msa0001460 MtrunA17_Chr1g0200181 26.494 385 246 11 121 496 143 499 1.44e-33 134
Msa0001460 MtrunA17_Chr5g0409981 28.010 407 258 14 110 510 91 468 2.86e-33 132
Msa0001460 MtrunA17_Chr2g0293631 28.093 388 247 12 112 497 485 842 3.01e-33 135
Msa0001460 MtrunA17_Chr1g0176041 28.186 408 246 14 121 511 152 529 1.50e-32 131
Msa0001460 MtrunA17_Chr6g0483491 26.829 410 255 11 121 524 8 378 1.50e-31 126
Msa0001460 MtrunA17_Chr6g0461751 24.868 378 255 9 123 498 140 490 6.69e-31 126
Msa0001460 MtrunA17_Chr7g0236841 26.098 410 258 11 121 524 8 378 1.09e-30 124
Msa0001460 MtrunA17_Chr4g0040761 29.201 363 222 11 145 504 41 371 8.98e-30 124
Msa0001460 MtrunA17_Chr7g0263771 25.823 395 252 13 121 509 127 486 9.64e-30 123
Msa0001460 MtrunA17_Chr7g0247191 27.851 377 232 11 113 486 140 479 1.18e-29 122
Msa0001460 MtrunA17_Chr2g0277821 26.289 388 241 12 121 498 502 854 1.24e-29 124
Msa0001460 MtrunA17_Chr2g0310581 27.644 416 249 15 104 509 38 411 1.44e-29 122
Msa0001460 MtrunA17_Chr7g0247181 27.851 377 232 11 113 486 130 469 1.96e-29 121
Msa0001460 MtrunA17_Chr7g0247221 27.851 377 232 11 113 486 133 472 2.17e-29 121
Msa0001460 MtrunA17_Chr3g0121351 25.616 406 260 14 121 511 143 521 4.12e-29 121
Msa0001460 MtrunA17_Chr4g0034411 28.800 375 220 17 146 509 99 437 4.68e-29 122
Msa0001460 MtrunA17_Chr6g0475251 29.915 351 205 12 148 488 128 447 1.29e-28 120
Msa0001460 MtrunA17_Chr7g0236831 26.364 440 229 15 133 504 173 585 5.83e-28 118
Msa0001460 MtrunA17_Chr3g0095031 25.628 398 245 11 127 513 94 451 7.74e-28 117
Msa0001460 MtrunA17_Chr3g0114081 28.684 380 224 16 148 507 41 393 7.89e-28 117
Msa0001460 MtrunA17_Chr5g0442191 25.234 428 275 14 92 513 54 442 7.93e-28 117
Msa0001460 MtrunA17_Chr5g0443991 27.346 373 229 15 146 509 99 438 9.75e-28 117
Msa0001460 MtrunA17_Chr2g0297261 25.743 404 261 10 112 509 17 387 2.80e-27 114
Msa0001460 MtrunA17_Chr1g0192021 25.547 411 233 13 122 487 25 407 3.22e-27 115
Msa0001460 MtrunA17_Chr4g0058591 26.912 353 222 11 121 468 200 521 6.91e-26 111
Msa0001460 MtrunA17_Chr4g0070981 27.083 384 231 11 123 498 41 383 4.65e-25 107
Msa0001460 MtrunA17_Chr4g0003441 27.889 398 231 12 124 507 109 464 5.25e-25 108
Msa0001460 MtrunA17_Chr2g0299681 27.461 386 227 12 123 498 41 383 8.32e-25 106
Msa0001460 MtrunA17_Chr2g0329931 26.750 400 239 13 112 498 25 383 1.10e-24 106
Msa0001460 MtrunA17_Chr4g0029191 27.111 450 248 19 111 496 32 465 1.35e-24 107
Msa0001460 MtrunA17_Chr8g0347161 25.103 486 288 17 33 492 24 459 1.44e-24 107
Msa0001460 MtrunA17_Chr1g0204391 27.473 364 213 10 148 488 69 404 4.28e-24 106
Msa0001460 MtrunA17_Chr8g0342861 26.044 407 247 13 112 505 25 390 5.18e-24 104
Msa0001460 MtrunA17_Chr4g0076491 25.127 394 252 15 121 496 285 653 2.17e-23 104
Msa0001460 MtrunA17_Chr1g0149251 28.070 342 194 10 167 496 8 309 2.01e-22 98.6
Msa0001460 MtrunA17_Chr8g0347171 24.550 444 280 16 67 492 111 517 1.21e-21 98.6
Msa0001460 MtrunA17_Chr8g0386451 25.000 448 281 17 102 525 261 677 1.40e-21 99.0
Msa0001460 MtrunA17_Chr6g0449741 24.611 386 247 13 121 489 247 605 1.97e-21 98.2
Msa0001460 MtrunA17_Chr8g0347151 25.598 418 262 16 86 492 179 558 7.39e-21 96.3
Msa0001460 MtrunA17_Chr2g0309551 24.570 407 262 10 119 489 105 502 4.57e-19 90.5
Msa0001460 MtrunA17_Chr6g0465771 30.994 171 108 3 124 293 93 254 3.84e-18 85.9
Msa0001460 MtrunA17_Chr4g0074521 23.704 405 245 12 112 486 71 441 2.24e-17 84.7
Msa0001460 MtrunA17_Chr8g0364251 32.432 185 109 6 309 489 358 530 1.02e-16 83.2
Msa0001460 MtrunA17_Chr8g0364251 30.457 197 112 8 110 288 72 261 2.35e-13 72.8
Msa0001460 MtrunA17_Chr0c09g0493491 29.609 179 116 3 124 301 93 262 1.29e-16 80.5
Msa0001460 MtrunA17_Chr4g0039471 30.542 203 124 6 123 321 223 412 2.18e-16 81.3
Msa0001460 MtrunA17_Chr8g0349951 28.571 168 103 3 120 287 7 157 1.22e-14 71.6
Msa0001460 MtrunA17_Chr5g0429221 22.113 407 278 14 121 523 1 372 2.01e-14 75.5
Msa0001460 MtrunA17_Chr1g0179761 24.048 499 284 21 33 502 21 453 2.30e-14 75.5
Msa0001460 MtrunA17_Chr2g0301281 30.435 184 114 6 116 291 34 211 3.66e-13 72.0
Msa0001460 MtrunA17_Chr4g0062321 24.268 239 143 8 234 462 23 233 1.39e-12 67.8
Msa0001460 MtrunA17_Chr0c30g0494091 25.664 226 134 5 112 320 71 279 6.01e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0001460 AT3G06980.1 66.519 451 139 3 88 528 331 779 0.0 636
Msa0001460 AT4G09730.1 31.490 416 244 12 121 522 111 499 7.08e-56 198
Msa0001460 AT1G55150.1 30.628 382 232 13 121 498 98 450 8.53e-43 159
Msa0001460 AT1G55150.2 30.628 382 232 13 121 498 123 475 1.96e-42 159
Msa0001460 AT5G63120.2 28.861 395 246 12 121 511 164 527 1.46e-38 149
Msa0001460 AT5G14610.6 28.534 382 235 13 121 497 160 508 3.95e-38 148
Msa0001460 AT5G14610.2 28.534 382 235 13 121 497 160 508 4.64e-38 148
Msa0001460 AT5G14610.5 28.534 382 235 13 121 497 168 516 4.94e-38 148
Msa0001460 AT5G14610.7 27.318 399 249 14 104 497 15 377 1.91e-36 142
Msa0001460 AT5G14610.3 27.318 399 249 14 104 497 15 377 1.91e-36 142
Msa0001460 AT3G01540.1 28.158 380 239 12 121 497 157 505 6.42e-36 142
Msa0001460 AT3G01540.4 28.158 380 239 12 121 497 157 505 7.32e-36 142
Msa0001460 AT3G01540.2 28.158 380 239 12 121 497 157 505 7.32e-36 142
Msa0001460 AT3G01540.3 28.158 380 239 12 121 497 157 505 7.32e-36 142
Msa0001460 AT2G47330.1 26.835 395 253 11 121 510 227 590 1.82e-35 141
Msa0001460 AT5G14610.1 28.689 366 223 13 137 497 244 576 3.73e-35 140
Msa0001460 AT1G31970.1 29.188 394 245 14 110 497 104 469 1.48e-34 137
Msa0001460 AT5G51280.1 26.632 383 248 11 121 496 145 501 2.72e-34 137
Msa0001460 AT5G65900.1 26.781 407 243 13 116 507 148 514 1.25e-33 135
Msa0001460 AT5G60990.1 27.540 374 241 10 121 489 8 356 4.47e-33 131
Msa0001460 AT2G42520.2 27.475 404 254 13 121 511 158 535 1.42e-32 132
Msa0001460 AT2G42520.1 27.475 404 254 13 121 511 158 535 1.42e-32 132
Msa0001460 AT2G42520.3 27.475 404 254 13 121 511 158 535 1.42e-32 132
Msa0001460 AT4G33370.1 26.371 383 249 11 121 496 96 452 1.82e-32 131
Msa0001460 AT5G26742.1 28.954 373 231 11 135 502 115 458 2.75e-32 132
Msa0001460 AT5G26742.2 28.954 373 231 11 135 502 115 458 2.76e-32 132
Msa0001460 AT3G06480.1 27.481 393 252 12 123 511 436 799 6.31e-32 131
Msa0001460 AT1G16280.1 26.847 406 253 13 113 511 51 419 9.15e-32 128
Msa0001460 AT4G00660.1 27.851 377 232 11 113 486 122 461 2.37e-31 127
Msa0001460 AT4G00660.2 27.851 377 232 11 113 486 122 461 2.37e-31 127
Msa0001460 AT3G58510.1 27.494 411 255 13 121 515 150 533 2.48e-31 128
Msa0001460 AT3G58510.3 27.494 411 255 13 121 515 150 533 2.48e-31 128
Msa0001460 AT3G58510.2 27.494 411 255 13 121 515 150 533 2.48e-31 128
Msa0001460 AT1G51380.1 26.720 378 244 10 121 498 21 365 3.57e-31 125
Msa0001460 AT3G18600.1 27.688 372 215 13 146 503 113 444 4.46e-31 127
Msa0001460 AT3G61240.2 28.142 366 223 10 124 486 126 454 5.20e-31 126
Msa0001460 AT3G61240.1 28.142 366 223 10 124 486 126 454 5.20e-31 126
Msa0001460 AT3G58570.1 27.518 407 250 14 121 511 145 522 5.62e-31 127
Msa0001460 AT5G26742.3 29.805 359 221 13 147 502 35 365 6.28e-31 127
Msa0001460 AT1G71370.1 28.643 398 219 14 133 507 27 382 6.33e-31 127
Msa0001460 AT1G20920.2 26.562 384 245 12 121 498 190 542 9.39e-31 127
Msa0001460 AT1G20920.4 26.562 384 245 12 121 498 528 880 1.88e-30 127
Msa0001460 AT1G20920.6 26.562 384 245 12 121 498 528 880 1.88e-30 127
Msa0001460 AT1G20920.3 26.562 384 245 12 121 498 528 880 1.88e-30 127
Msa0001460 AT1G20920.7 26.562 384 245 12 121 498 528 880 1.88e-30 127
Msa0001460 AT1G20920.5 26.562 384 245 12 121 498 528 880 1.88e-30 127
Msa0001460 AT1G20920.1 26.562 384 245 12 121 498 528 880 1.88e-30 127
Msa0001460 AT5G14610.4 27.221 349 217 12 121 464 168 484 5.93e-30 123
Msa0001460 AT2G45810.1 27.007 411 259 11 80 486 111 484 1.02e-29 123
Msa0001460 AT3G09720.2 26.582 395 249 13 121 509 136 495 2.08e-29 122
Msa0001460 AT3G09720.1 26.582 395 249 13 121 509 136 495 2.08e-29 122
Msa0001460 AT1G77030.1 28.571 357 220 10 145 498 51 375 2.76e-29 123
Msa0001460 AT3G22330.1 25.490 408 262 12 123 524 105 476 3.88e-29 122
Msa0001460 AT5G54910.1 26.327 471 285 18 65 511 3 435 5.41e-29 122
Msa0001460 AT5G63120.1 28.739 341 209 11 121 457 164 474 6.78e-29 120
Msa0001460 AT4G16630.1 28.133 391 246 12 119 505 164 523 9.15e-29 121
Msa0001460 AT5G63120.3 28.739 341 209 11 121 457 164 474 1.05e-28 119
Msa0001460 AT5G63120.4 28.739 341 209 11 121 457 164 474 1.05e-28 119
Msa0001460 AT5G63120.5 28.739 341 209 11 121 457 164 474 1.05e-28 119
Msa0001460 AT3G02065.3 27.865 384 237 13 123 498 111 462 2.52e-28 118
Msa0001460 AT3G02065.2 27.865 384 237 13 123 498 111 462 2.52e-28 118
Msa0001460 AT3G22310.2 25.000 408 264 12 123 524 117 488 8.74e-28 117
Msa0001460 AT3G22310.1 25.000 408 264 12 123 524 117 488 9.34e-28 117
Msa0001460 AT5G05450.1 26.429 420 251 15 105 502 3 386 1.46e-27 117
Msa0001460 AT3G19760.1 25.707 389 256 9 121 509 34 389 3.18e-27 114
Msa0001460 AT3G09620.1 25.393 382 217 11 124 498 398 718 3.83e-27 116
Msa0001460 AT3G09620.2 25.393 382 217 11 124 498 399 719 3.98e-27 116
Msa0001460 AT3G02065.1 28.329 353 223 12 148 498 1 325 5.98e-27 112
Msa0001460 AT2G33730.1 24.816 407 273 12 121 513 312 699 8.63e-25 109
Msa0001460 AT5G11170.1 26.873 387 227 12 124 496 48 392 2.30e-24 105
Msa0001460 AT5G11200.1 26.873 387 227 12 124 496 48 392 2.30e-24 105
Msa0001460 AT4G34910.1 24.390 410 241 11 122 487 46 430 2.67e-24 107
Msa0001460 AT3G06480.2 25.648 347 229 10 123 468 436 754 5.12e-24 107
Msa0001460 AT3G13920.5 27.083 384 233 10 122 498 43 386 6.08e-24 104
Msa0001460 AT3G13920.1 27.083 384 233 10 122 498 39 382 6.14e-24 104
Msa0001460 AT3G13920.3 27.083 384 233 10 122 498 29 372 7.01e-24 104
Msa0001460 AT3G13920.2 27.083 384 233 10 122 498 39 382 7.94e-24 104
Msa0001460 AT1G54270.1 25.995 377 246 8 122 498 39 382 1.59e-23 103
Msa0001460 AT3G16840.1 26.406 409 236 13 132 487 200 596 2.79e-23 104
Msa0001460 AT3G16840.2 26.406 409 236 13 132 487 201 597 2.94e-23 104
Msa0001460 AT1G28180.1 28.451 355 213 13 168 513 19 341 4.73e-23 101
Msa0001460 AT2G07750.1 25.500 400 251 15 117 499 370 739 6.17e-23 103
Msa0001460 AT2G40700.1 25.652 460 261 22 103 496 10 454 1.83e-22 101
Msa0001460 AT5G62190.1 26.374 364 201 9 148 488 122 441 1.90e-22 101
Msa0001460 AT1G12770.2 23.666 431 283 11 118 513 106 525 3.07e-22 100
Msa0001460 AT1G12770.1 23.666 431 283 11 118 513 106 525 3.07e-22 100
Msa0001460 AT5G08610.1 26.031 388 234 15 119 492 392 740 3.74e-22 101
Msa0001460 AT1G72730.1 26.471 374 226 11 133 498 52 384 4.89e-22 99.0
Msa0001460 AT3G13920.4 27.083 384 228 11 122 498 39 377 1.01e-21 97.8
Msa0001460 AT1G54270.2 25.995 377 241 9 122 498 39 377 1.56e-21 97.4
Msa0001460 AT5G11170.2 27.907 344 192 12 167 496 8 309 1.81e-21 96.3
Msa0001460 AT5G11200.3 25.476 420 232 14 124 496 48 433 2.05e-21 97.8
Msa0001460 AT1G63250.1 24.812 399 255 15 117 499 323 692 9.67e-21 96.7
Msa0001460 AT5G11200.2 23.666 431 244 13 124 496 48 451 6.05e-20 93.2
Msa0001460 AT5G63630.1 25.064 391 234 13 119 492 64 412 7.37e-20 93.2
Msa0001460 AT5G63630.2 25.316 395 228 16 119 492 258 606 8.58e-20 93.6
Msa0001460 AT5G08620.1 24.112 394 261 12 112 492 70 438 3.37e-19 91.3
Msa0001460 AT1G51380.2 24.632 272 182 5 121 392 21 269 1.18e-18 86.7
Msa0001460 AT5G19210.2 24.934 377 235 14 131 486 85 434 1.23e-18 89.4
Msa0001460 AT1G51380.4 28.293 205 127 4 121 321 21 209 7.73e-17 81.6
Msa0001460 AT1G51380.3 28.293 205 127 4 121 321 21 209 8.96e-17 81.6
Msa0001460 AT3G53110.1 23.934 422 271 13 110 515 79 466 5.27e-16 81.3
Msa0001460 AT1G59990.2 27.778 216 132 6 309 520 354 549 6.99e-15 77.8
Msa0001460 AT1G59990.1 27.778 216 132 6 309 520 354 549 1.00e-14 77.4

Find 138 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGAAGTGTCCCAGTTTA+TGG 0.122594 1_1:+3158743 Msa0001460:CDS
CAGCATCTTTAGATGGTCTT+TGG 0.216057 1_1:+3159345 Msa0001460:three_prime_UTR
ATATTACTTAGTAACTTCTT+AGG 0.277798 1_1:+3159392 Msa0001460:three_prime_UTR
ATTGCTGACCAGAGTGGTTC+TGG 0.306260 1_1:+3156815 Msa0001460:CDS
GCCCTGTCCCCTGGCACCTC+TGG 0.308184 1_1:-3159014 None:intergenic
TAGACATTGAACGACAATTA+TGG 0.323737 1_1:-3156973 None:intergenic
TTGGAAAGGGTGCTCTCAAC+TGG 0.323947 1_1:+3159185 Msa0001460:three_prime_UTR
TCATGAAAATAAAATTTGAT+TGG 0.327018 1_1:-3159483 None:intergenic
ATAAACTGGGACACTTCTTT+TGG 0.327753 1_1:-3158741 None:intergenic
TGTCCGTGCAAACCATAAAC+TGG 0.332471 1_1:-3158755 None:intergenic
TTGCAGAACACAATAGTTCT+TGG 0.346234 1_1:-3158476 None:intergenic
TCATTTGCTCCTGTTATTGC+CGG 0.349281 1_1:+3156779 Msa0001460:CDS
AGCATCTTTAGATGGTCTTT+GGG 0.352962 1_1:+3159346 Msa0001460:three_prime_UTR
GATGAGGTTGATATCCTCTT+TGG 0.353589 1_1:+3157327 Msa0001460:CDS
GGTAGCACCGACAATATTCT+AGG 0.364067 1_1:+3159264 Msa0001460:three_prime_UTR
GGCGAGTTAAAGAGAGGTTA+TGG 0.368794 1_1:-3156736 None:intergenic
CAAGACTTTCCGGCAATAAC+AGG 0.372706 1_1:-3156788 None:intergenic
AAAAGAGAAGAGAATCAAAA+TGG 0.386747 1_1:+3156307 Msa0001460:exon
AGCAATTATACAAGACTTTC+CGG 0.389114 1_1:-3156798 None:intergenic
GGCAAAGGAAGGAAATCTTT+TGG 0.389304 1_1:+3159243 Msa0001460:three_prime_UTR
CATGAAAATAAAATTTGATT+GGG 0.393804 1_1:-3159482 None:intergenic
AGCCAGAGGTGCCAGGGGAC+AGG 0.398550 1_1:+3159012 Msa0001460:CDS
GTCGATAAAACCACGAGTTT+CGG 0.403323 1_1:+3156458 Msa0001460:CDS
GAAGAAGACAGAGTGTTTAC+TGG 0.408498 1_1:-3156393 None:intergenic
CACAGCTGAGTTAGCTTCTC+AGG 0.409704 1_1:+3156946 Msa0001460:CDS
AGTTACTAAGTAATATGATT+AGG 0.415417 1_1:-3159385 None:intergenic
TGTCGTTCAATGTCTAAATC+TGG 0.417609 1_1:+3156980 Msa0001460:CDS
GAGTTGGATGGGGAGACAAT+AGG 0.426703 1_1:+3156612 Msa0001460:CDS
AAGGCATTTATCTTTGTTGT+TGG 0.426934 1_1:+3159037 Msa0001460:CDS
TGCAGAACACAATAGTTCTT+GGG 0.438418 1_1:-3158475 None:intergenic
CCCATGACCATTTCACAATC+AGG 0.439831 1_1:-3157471 None:intergenic
GAGCTGAAGAAGTAGGAAGT+TGG 0.443767 1_1:+3159148 Msa0001460:three_prime_UTR
TGATTGCCAGCATCTTTAGA+TGG 0.444955 1_1:+3159338 Msa0001460:three_prime_UTR
TCCACTGCATGATGTACCTT+CGG 0.449294 1_1:+3159111 Msa0001460:CDS
AGTTGGATGGGGAGACAATA+GGG 0.452318 1_1:+3156613 Msa0001460:CDS
GAAGAACAGCCAGAGGTGCC+AGG 0.461221 1_1:+3159005 Msa0001460:CDS
CTAAAATCATTTAAAGACAT+TGG 0.461700 1_1:+3156686 Msa0001460:CDS
AGTGAAACCTGACCTCTTCA+AGG 0.469671 1_1:-3157521 None:intergenic
AACTCGCCCTTCCAACATAC+AGG 0.470926 1_1:+3156751 Msa0001460:CDS
TAGGGTTAGCAGTCAATCAT+TGG 0.471037 1_1:+3156631 Msa0001460:CDS
AACTCATTCGAGAGTTGGAT+GGG 0.471462 1_1:+3156601 Msa0001460:CDS
AAATGCCTTGCCCTGTCCCC+TGG 0.473330 1_1:-3159023 None:intergenic
TGTCTGAGACGTTGAATGAT+TGG 0.476607 1_1:-3156860 None:intergenic
AGTTTATGGTTTGCACGGAC+AGG 0.484625 1_1:+3158757 Msa0001460:CDS
AACTTGTAACAGAGCTTCGC+GGG 0.488229 1_1:+3158904 Msa0001460:intron
AGGTGCCAGGGGACAGGGCA+AGG 0.488567 1_1:+3159018 Msa0001460:CDS
GAATTCCCTTTCTATCAAAA+CGG 0.499892 1_1:-3158638 None:intergenic
TCTTTAGATGGTCTTTGGGC+AGG 0.501801 1_1:+3159350 Msa0001460:three_prime_UTR
GCCAGAGGTGCCAGGGGACA+GGG 0.502782 1_1:+3159013 Msa0001460:CDS
ATTATGTTGCAGTGCTGTCT+TGG 0.503149 1_1:+3157305 Msa0001460:intron
TTGCTGTTTAGCATCGGAGT+TGG 0.503766 1_1:+3156362 Msa0001460:CDS
TCCTGATTGTGAAATGGTCA+TGG 0.506213 1_1:+3157470 Msa0001460:CDS
GTAGATTGTAGTGGGGAAGA+TGG 0.506554 1_1:+3158377 Msa0001460:CDS
AGCTGCTCTTCTGCAGCTTG+CGG 0.507830 1_1:+3158439 Msa0001460:CDS
TTTAATTGATTGCTTGGAAA+GGG 0.510457 1_1:+3159172 Msa0001460:three_prime_UTR
TCATCGACCTAGAATATTGT+CGG 0.512080 1_1:-3159271 None:intergenic
TGGCGATGAGGACTTCGAAA+AGG 0.514933 1_1:+3157347 Msa0001460:CDS
AGATAATAGTAGATTGTAGT+GGG 0.515530 1_1:+3158369 Msa0001460:intron
ATGCTTGATTAGTTCTTCAC+CGG 0.515910 1_1:+3157377 Msa0001460:CDS
CAACTCATTCGAGAGTTGGA+TGG 0.516133 1_1:+3156600 Msa0001460:CDS
CAAAGACCATCTAAAGATGC+TGG 0.522860 1_1:-3159344 None:intergenic
GGTTGATGAGGAAGAGAAAA+CGG 0.523534 1_1:-3157105 None:intergenic
TCAATCATTGGCACGGGCAT+CGG 0.526837 1_1:+3156643 Msa0001460:CDS
ACTGCAACTCATTCGAGAGT+TGG 0.528094 1_1:+3156596 Msa0001460:CDS
GGCTCCTCATAGCAAAAGCA+AGG 0.533958 1_1:+3159308 Msa0001460:three_prime_UTR
AGTGAATATGTACGGCGTGT+TGG 0.534281 1_1:+3158983 Msa0001460:CDS
CCTGATTGTGAAATGGTCAT+GGG 0.535391 1_1:+3157471 Msa0001460:CDS
GTCCCAGTTTATGGTTTGCA+CGG 0.536407 1_1:+3158752 Msa0001460:CDS
GTGACAAGCATAGATTTGAA+TGG 0.537448 1_1:-3157010 None:intergenic
CTTAGGAATTGTATTACCAC+CGG 0.538984 1_1:+3159409 Msa0001460:three_prime_UTR
AAATCTATGCTTGTCACTGG+TGG 0.541886 1_1:+3157016 Msa0001460:CDS
ATCACGGCTTACTAGTATGA+AGG 0.552151 1_1:+3158701 Msa0001460:CDS
TTGCAGTGCTGTCTTGGATG+AGG 0.552233 1_1:+3157311 Msa0001460:intron
CGAAGTCCTCATCGCCAAAG+AGG 0.554253 1_1:-3157341 None:intergenic
CAGATAATAGTAGATTGTAG+TGG 0.557322 1_1:+3158368 Msa0001460:intron
TTCAAATCTATGCTTGTCAC+TGG 0.558308 1_1:+3157013 Msa0001460:CDS
GTGGTTGCGCAAGGTGTTTG+TGG 0.559691 1_1:+3156332 Msa0001460:CDS
GTATTGAGAGCTGAAGAAGT+AGG 0.560738 1_1:+3159141 Msa0001460:exon
TGGAAGGGCGAGTTAAAGAG+AGG 0.560834 1_1:-3156742 None:intergenic
AAATGACATGGCCTGTATGT+TGG 0.565806 1_1:-3156762 None:intergenic
AGTAATGGAAAGAAATCGGA+AGG 0.566217 1_1:+3159084 Msa0001460:CDS
TCGATGAAACGTGCATTGCT+AGG 0.568808 1_1:+3159287 Msa0001460:three_prime_UTR
AAGAACAGCCAGAGGTGCCA+GGG 0.570215 1_1:+3159006 Msa0001460:CDS
GAACTTATTCGATGCATACT+AGG 0.571644 1_1:-3157495 None:intergenic
AATAAATATTGTGTGTCCAC+CGG 0.571880 1_1:-3157396 None:intergenic
CAACTTGTAACAGAGCTTCG+CGG 0.572338 1_1:+3158903 Msa0001460:intron
TGGAAAGGGTGCTCTCAACT+GGG 0.579482 1_1:+3159186 Msa0001460:three_prime_UTR
AAGTCTAACATGCATGGCAA+AGG 0.579671 1_1:+3159228 Msa0001460:three_prime_UTR
CTTGATTAGTTCTTCACCGG+TGG 0.581413 1_1:+3157380 Msa0001460:CDS
AAAGCGTTGCTGTTTAGCAT+CGG 0.582268 1_1:+3156356 Msa0001460:CDS
TGTGTCATGGCAGCATGGAA+AGG 0.582945 1_1:-3158675 None:intergenic
ATCATTGGCACGGGCATCGG+AGG 0.583854 1_1:+3156646 Msa0001460:CDS
GGTGCGAGTATAACAAGAGT+AGG 0.587052 1_1:-3156923 None:intergenic
CTCAGACAACAAGAACTACA+AGG 0.588339 1_1:+3156875 Msa0001460:CDS
TGATATCCTCTTTGGCGATG+AGG 0.588829 1_1:+3157335 Msa0001460:CDS
CTAACATGCATGGCAAAGGA+AGG 0.589079 1_1:+3159232 Msa0001460:three_prime_UTR
TGCACTTAACTAATCTAACA+TGG 0.590356 1_1:+3157137 Msa0001460:CDS
ATGTCTTTCCAGAACCACTC+TGG 0.590455 1_1:-3156823 None:intergenic
AATAAGTTCTCGCCTTGAAG+AGG 0.590513 1_1:+3157509 Msa0001460:CDS
TGCAGGAGTGTCATTTCCGG+TGG 0.590891 1_1:-3159425 None:intergenic
TAGCAGTCAATCATTGGCAC+GGG 0.591577 1_1:+3156637 Msa0001460:CDS
CTGAGAAGCTAACTCAGCTG+TGG 0.591686 1_1:-3156945 None:intergenic
TTTCTCTTCCTCATCAACCA+AGG 0.592789 1_1:+3157109 Msa0001460:CDS
AAGCCGTGATTCTTGTGTCA+TGG 0.593997 1_1:-3158688 None:intergenic
TATTGTAAGTCTAACATGCA+TGG 0.594332 1_1:+3159222 Msa0001460:three_prime_UTR
ACACGCCGTACATATTCACT+TGG 0.594509 1_1:-3158980 None:intergenic
GGGAATTGACTTCACTAGAG+TGG 0.597817 1_1:+3158925 Msa0001460:CDS
TCAACTCGACACTTTAAACC+AGG 0.598198 1_1:+3157054 Msa0001460:CDS
AAGAAGACAGAGTGTTTACT+GGG 0.599621 1_1:-3156392 None:intergenic
TTAGCAGTCAATCATTGGCA+CGG 0.601798 1_1:+3156636 Msa0001460:CDS
GTCCGTGCAAACCATAAACT+GGG 0.602781 1_1:-3158754 None:intergenic
ACATATTCACTTGGATCACG+TGG 0.605024 1_1:-3158971 None:intergenic
GTGATCCAAGTGAATATGTA+CGG 0.607674 1_1:+3158975 Msa0001460:CDS
TTCAATGTCTAAATCTGGCA+AGG 0.610738 1_1:+3156985 Msa0001460:CDS
ATGAGGAAGAGAAAACGGCC+AGG 0.612771 1_1:-3157100 None:intergenic
TGAGAAGCTAACTCAGCTGT+GGG 0.613832 1_1:-3156944 None:intergenic
AAAATGGCGGTGGTTGCGCA+AGG 0.615353 1_1:+3156323 Msa0001460:exon
GAAGAGAATCAAAATGGCGG+TGG 0.615716 1_1:+3156313 Msa0001460:exon
GACATGGCCTGTATGTTGGA+AGG 0.625996 1_1:-3156758 None:intergenic
GATGTCTTCATAGCTACACC+TGG 0.633711 1_1:+3157082 Msa0001460:CDS
CTGCCATGACACAAGAATCA+CGG 0.635761 1_1:+3158685 Msa0001460:CDS
GATAATAGTAGATTGTAGTG+GGG 0.637024 1_1:+3158370 Msa0001460:intron
AGAACAGCCAGAGGTGCCAG+GGG 0.638006 1_1:+3159007 Msa0001460:CDS
GCCGAAGGTACATCATGCAG+TGG 0.640301 1_1:-3159112 None:intergenic
TGTATAATTGCTGACCAGAG+TGG 0.648838 1_1:+3156809 Msa0001460:CDS
TCTCAATACTCATAAGCCGA+AGG 0.655130 1_1:-3159127 None:intergenic
ACATGGCCTGTATGTTGGAA+GGG 0.662222 1_1:-3156757 None:intergenic
ATTCTTGTGTCATGGCAGCA+TGG 0.663708 1_1:-3158680 None:intergenic
ACTCATTCGAGAGTTGGATG+GGG 0.667637 1_1:+3156602 Msa0001460:CDS
CGTGTTGGAAGAACAGCCAG+AGG 0.669248 1_1:+3158998 Msa0001460:CDS
GCTCCTCATAGCAAAAGCAA+GGG 0.695618 1_1:+3159309 Msa0001460:three_prime_UTR
CAACTCGACACTTTAAACCA+GGG 0.695695 1_1:+3157055 Msa0001460:CDS
GTAATGGAAAGAAATCGGAA+GGG 0.700702 1_1:+3159085 Msa0001460:CDS
AATAACAGGAGCAAATGACA+TGG 0.700797 1_1:-3156774 None:intergenic
ACTTGTAACAGAGCTTCGCG+GGG 0.711965 1_1:+3158905 Msa0001460:intron
CATATTCACTTGGATCACGT+GGG 0.715544 1_1:-3158970 None:intergenic
AGAGAAGAGAATCAAAATGG+CGG 0.724812 1_1:+3156310 Msa0001460:exon
CTATGAAGACATCAACACCC+TGG 0.726139 1_1:-3157072 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTCATAATATGAAATAAA+AGG + chr1_1:3157955-3157974 Msa0001460:intron 10.0%
!! ATTCATAATATGAAATAAAA+GGG + chr1_1:3157956-3157975 Msa0001460:intron 10.0%
!!! AATATTAATTTGAAATTAAG+TGG - chr1_1:3158265-3158284 None:intergenic 10.0%
!! CAAAAAATTACAAATATAAT+AGG + chr1_1:3159446-3159465 Msa0001460:three_prime_UTR 10.0%
!! GTTAAGATAAATTAAATCAT+TGG - chr1_1:3157275-3157294 None:intergenic 15.0%
!!! GTTATTGTTTTACTATTTAT+AGG + chr1_1:3158027-3158046 Msa0001460:intron 15.0%
!! TAATATATTCTTAAATTGCT+GGG + chr1_1:3158192-3158211 Msa0001460:intron 15.0%
!!! CTTTTTGTTACTTTTTATTT+TGG + chr1_1:3158554-3158573 Msa0001460:intron 15.0%
!!! TTATATTTGTAATTTTTTGC+AGG - chr1_1:3159445-3159464 None:intergenic 15.0%
!! CATGAAAATAAAATTTGATT+GGG - chr1_1:3159485-3159504 None:intergenic 15.0%
!! TCATGAAAATAAAATTTGAT+TGG - chr1_1:3159486-3159505 None:intergenic 15.0%
!!! TTTCAATTTTCAACAAGATA+AGG - chr1_1:3156276-3156295 None:intergenic 20.0%
!! CTAAAATCATTTAAAGACAT+TGG + chr1_1:3156686-3156705 Msa0001460:CDS 20.0%
!! AGAAATCAATTTATAACACA+TGG - chr1_1:3157239-3157258 None:intergenic 20.0%
!!! CTTTCTATTTTATTCATCTT+AGG + chr1_1:3157558-3157577 Msa0001460:intron 20.0%
!! ATTCAATTTAAATCTCATGT+GGG - chr1_1:3157672-3157691 None:intergenic 20.0%
!! AATTCAATTTAAATCTCATG+TGG - chr1_1:3157673-3157692 None:intergenic 20.0%
!! ATACACAATGATATGTATAA+AGG - chr1_1:3157711-3157730 None:intergenic 20.0%
!! TTTATACATATCATTGTGTA+TGG + chr1_1:3157710-3157729 Msa0001460:intron 20.0%
!! TCCAGTATTAAATTTATTAG+TGG - chr1_1:3157874-3157893 None:intergenic 20.0%
!!! GTATTTCTTTTTGAATAGTA+TGG - chr1_1:3157899-3157918 None:intergenic 20.0%
!!! GAAATTTTGAGAAATGTTTA+GGG - chr1_1:3157929-3157948 None:intergenic 20.0%
!!! TGAAATTTTGAGAAATGTTT+AGG - chr1_1:3157930-3157949 None:intergenic 20.0%
!! TTGTACTAGTTGTATATAAT+TGG - chr1_1:3158007-3158026 None:intergenic 20.0%
!! GCAATTTAAGAATATATTAC+AGG - chr1_1:3158191-3158210 None:intergenic 20.0%
!! GTAATATATTCTTAAATTGC+TGG + chr1_1:3158191-3158210 Msa0001460:intron 20.0%
!! AATATATTCTTAAATTGCTG+GGG + chr1_1:3158193-3158212 Msa0001460:intron 20.0%
!!! AAAAAGTAATGGAAAGAAAT+CGG + chr1_1:3159080-3159099 Msa0001460:CDS 20.0%
!! AGTTACTAAGTAATATGATT+AGG - chr1_1:3159388-3159407 None:intergenic 20.0%
!! ATATTACTTAGTAACTTCTT+AGG + chr1_1:3159392-3159411 Msa0001460:three_prime_UTR 20.0%
! AAAAGAGAAGAGAATCAAAA+TGG + chr1_1:3156307-3156326 Msa0001460:exon 25.0%
!! TGATTTTAGACTGAAAAAGT+TGG - chr1_1:3156675-3156694 None:intergenic 25.0%
! TTAACTAATCTAACATGGTA+TGG + chr1_1:3157142-3157161 Msa0001460:intron 25.0%
! AAAATGATAGGTGATAGAAA+AGG - chr1_1:3157185-3157204 None:intergenic 25.0%
! GATAAATTAAATCATTGGTG+CGG - chr1_1:3157270-3157289 None:intergenic 25.0%
!!! AGTTTGCTGTAAACATTTTT+TGG - chr1_1:3157438-3157457 None:intergenic 25.0%
!!! GTTGGTAACAATTATGAAAA+TGG + chr1_1:3157746-3157765 Msa0001460:intron 25.0%
! GCCACTAATAAATTTAATAC+TGG + chr1_1:3157870-3157889 Msa0001460:intron 25.0%
! AGATAATAGTAGATTGTAGT+GGG + chr1_1:3158369-3158388 Msa0001460:intron 25.0%
!!! ATTAAACCGTTTTGATAGAA+AGG + chr1_1:3158632-3158651 Msa0001460:CDS 25.0%
!!! TTAAACCGTTTTGATAGAAA+GGG + chr1_1:3158633-3158652 Msa0001460:CDS 25.0%
! GTGTTACAAATAATTCATTG+TGG + chr1_1:3158880-3158899 Msa0001460:intron 25.0%
!!! TTTTAATTGATTGCTTGGAA+AGG + chr1_1:3159171-3159190 Msa0001460:three_prime_UTR 25.0%
! TTTAATTGATTGCTTGGAAA+GGG + chr1_1:3159172-3159191 Msa0001460:three_prime_UTR 25.0%
AGCAATTATACAAGACTTTC+CGG - chr1_1:3156801-3156820 None:intergenic 30.0%
TAGACATTGAACGACAATTA+TGG - chr1_1:3156976-3156995 None:intergenic 30.0%
TGCACTTAACTAATCTAACA+TGG + chr1_1:3157137-3157156 Msa0001460:CDS 30.0%
TAATCTAACATGGTATGGTA+TGG + chr1_1:3157147-3157166 Msa0001460:intron 30.0%
GGTTTAGTCAACAAAATGAT+AGG - chr1_1:3157197-3157216 None:intergenic 30.0%
AATAAATATTGTGTGTCCAC+CGG - chr1_1:3157399-3157418 None:intergenic 30.0%
! AGTTTTTCCTGATTGTGAAA+TGG + chr1_1:3157464-3157483 Msa0001460:CDS 30.0%
CTGTGATATGAGAAAACATT+TGG + chr1_1:3157630-3157649 Msa0001460:intron 30.0%
AGGGATAGTAAAATCGAAAA+TGG + chr1_1:3157975-3157994 Msa0001460:intron 30.0%
TGAAATTAAGTGGTCATTAC+TGG - chr1_1:3158255-3158274 None:intergenic 30.0%
CAGATAATAGTAGATTGTAG+TGG + chr1_1:3158368-3158387 Msa0001460:intron 30.0%
GATAATAGTAGATTGTAGTG+GGG + chr1_1:3158370-3158389 Msa0001460:intron 30.0%
GAATTCCCTTTCTATCAAAA+CGG - chr1_1:3158641-3158660 None:intergenic 30.0%
! AAGGCATTTATCTTTGTTGT+TGG + chr1_1:3159037-3159056 Msa0001460:CDS 30.0%
!!! TTCCATTACTTTTTGTGCAA+GGG - chr1_1:3159074-3159093 None:intergenic 30.0%
!!! TTTCCATTACTTTTTGTGCA+AGG - chr1_1:3159075-3159094 None:intergenic 30.0%
!!! GTTGGTTTTAATTGATTGCT+TGG + chr1_1:3159166-3159185 Msa0001460:three_prime_UTR 30.0%
TATTGTAAGTCTAACATGCA+TGG + chr1_1:3159222-3159241 Msa0001460:three_prime_UTR 30.0%
AGAGAAGAGAATCAAAATGG+CGG + chr1_1:3156310-3156329 Msa0001460:exon 35.0%
! AAGAAGACAGAGTGTTTACT+GGG - chr1_1:3156395-3156414 None:intergenic 35.0%
!! GGCGGATTTTGAATTTGAAT+TGG - chr1_1:3156438-3156457 None:intergenic 35.0%
! TTGTAGTTGTTTGCGTTTTG+TGG - chr1_1:3156537-3156556 None:intergenic 35.0%
AATAACAGGAGCAAATGACA+TGG - chr1_1:3156777-3156796 None:intergenic 35.0%
TGTCGTTCAATGTCTAAATC+TGG + chr1_1:3156980-3156999 Msa0001460:CDS 35.0%
TTCAATGTCTAAATCTGGCA+AGG + chr1_1:3156985-3157004 Msa0001460:CDS 35.0%
GTGACAAGCATAGATTTGAA+TGG - chr1_1:3157013-3157032 None:intergenic 35.0%
TTCAAATCTATGCTTGTCAC+TGG + chr1_1:3157013-3157032 Msa0001460:CDS 35.0%
!!! TGTCGAGTTGAGTTTTTTGT+CGG - chr1_1:3157045-3157064 None:intergenic 35.0%
TAGTTAAGTGCAAAAAGCCT+TGG - chr1_1:3157129-3157148 None:intergenic 35.0%
TAACATGGTATGGTATGGTA+TGG + chr1_1:3157152-3157171 Msa0001460:intron 35.0%
GGTAAATGACAAGTGTATTC+AGG - chr1_1:3157218-3157237 None:intergenic 35.0%
! ATGCTTGATTAGTTCTTCAC+CGG + chr1_1:3157377-3157396 Msa0001460:CDS 35.0%
GAACTTATTCGATGCATACT+AGG - chr1_1:3157498-3157517 None:intergenic 35.0%
AACGTATTCCCTAAATTTGC+TGG + chr1_1:3157595-3157614 Msa0001460:intron 35.0%
TACTATTTATAGGTCCGCTT+TGG + chr1_1:3158037-3158056 Msa0001460:intron 35.0%
!! CTTAAATTGCTGGGGTATTT+TGG + chr1_1:3158201-3158220 Msa0001460:intron 35.0%
TGCAGAACACAATAGTTCTT+GGG - chr1_1:3158478-3158497 None:intergenic 35.0%
TTGCAGAACACAATAGTTCT+TGG - chr1_1:3158479-3158498 None:intergenic 35.0%
! ACAAAGTATTCTGAACTCTC+AGG + chr1_1:3158498-3158517 Msa0001460:intron 35.0%
! ATAAACTGGGACACTTCTTT+TGG - chr1_1:3158744-3158763 None:intergenic 35.0%
!! AAAAGAAGTGTCCCAGTTTA+TGG + chr1_1:3158743-3158762 Msa0001460:CDS 35.0%
TATTCATCTGTTTATCTCGC+TGG + chr1_1:3158780-3158799 Msa0001460:intron 35.0%
GTGATCCAAGTGAATATGTA+CGG + chr1_1:3158975-3158994 Msa0001460:CDS 35.0%
ATCCCTTGCACAAAAAGTAA+TGG + chr1_1:3159069-3159088 Msa0001460:CDS 35.0%
! AGTAATGGAAAGAAATCGGA+AGG + chr1_1:3159084-3159103 Msa0001460:CDS 35.0%
! GTAATGGAAAGAAATCGGAA+GGG + chr1_1:3159085-3159104 Msa0001460:CDS 35.0%
TCATCGACCTAGAATATTGT+CGG - chr1_1:3159274-3159293 None:intergenic 35.0%
AGCATCTTTAGATGGTCTTT+GGG + chr1_1:3159346-3159365 Msa0001460:three_prime_UTR 35.0%
CTTAGGAATTGTATTACCAC+CGG + chr1_1:3159409-3159428 Msa0001460:three_prime_UTR 35.0%
!! AAAGCGTTGCTGTTTAGCAT+CGG + chr1_1:3156356-3156375 Msa0001460:CDS 40.0%
! GAAGAAGACAGAGTGTTTAC+TGG - chr1_1:3156396-3156415 None:intergenic 40.0%
!!! GAAACTCGTGGTTTTATCGA+CGG - chr1_1:3156459-3156478 None:intergenic 40.0%
GTCGATAAAACCACGAGTTT+CGG + chr1_1:3156458-3156477 Msa0001460:CDS 40.0%
!! TTTTAGTCTTCCGAAACTCG+TGG - chr1_1:3156471-3156490 None:intergenic 40.0%
!!! TGGTATTTTGCTTGGTTCTG+TGG - chr1_1:3156517-3156536 None:intergenic 40.0%
! GCGTTTTGTGGTATTTTGCT+TGG - chr1_1:3156525-3156544 None:intergenic 40.0%
AACTCATTCGAGAGTTGGAT+GGG + chr1_1:3156601-3156620 Msa0001460:CDS 40.0%
! TAGGGTTAGCAGTCAATCAT+TGG + chr1_1:3156631-3156650 Msa0001460:CDS 40.0%
TTAGCAGTCAATCATTGGCA+CGG + chr1_1:3156636-3156655 Msa0001460:CDS 40.0%
AAATGACATGGCCTGTATGT+TGG - chr1_1:3156765-3156784 None:intergenic 40.0%
TCATTTGCTCCTGTTATTGC+CGG + chr1_1:3156779-3156798 Msa0001460:CDS 40.0%
TGTATAATTGCTGACCAGAG+TGG + chr1_1:3156809-3156828 Msa0001460:CDS 40.0%
! TGTCTGAGACGTTGAATGAT+TGG - chr1_1:3156863-3156882 None:intergenic 40.0%
CTCAGACAACAAGAACTACA+AGG + chr1_1:3156875-3156894 Msa0001460:CDS 40.0%
AAATCTATGCTTGTCACTGG+TGG + chr1_1:3157016-3157035 Msa0001460:CDS 40.0%
TCAACTCGACACTTTAAACC+AGG + chr1_1:3157054-3157073 Msa0001460:CDS 40.0%
CAACTCGACACTTTAAACCA+GGG + chr1_1:3157055-3157074 Msa0001460:CDS 40.0%
!! GGTTGATGAGGAAGAGAAAA+CGG - chr1_1:3157108-3157127 None:intergenic 40.0%
TTTCTCTTCCTCATCAACCA+AGG + chr1_1:3157109-3157128 Msa0001460:CDS 40.0%
ATTATGTTGCAGTGCTGTCT+TGG + chr1_1:3157305-3157324 Msa0001460:intron 40.0%
!! GATGAGGTTGATATCCTCTT+TGG + chr1_1:3157327-3157346 Msa0001460:CDS 40.0%
TCCTGATTGTGAAATGGTCA+TGG + chr1_1:3157470-3157489 Msa0001460:CDS 40.0%
CCTGATTGTGAAATGGTCAT+GGG + chr1_1:3157471-3157490 Msa0001460:CDS 40.0%
AATAAGTTCTCGCCTTGAAG+AGG + chr1_1:3157509-3157528 Msa0001460:CDS 40.0%
!! TATTCCCTAAATTTGCTGGC+TGG + chr1_1:3157599-3157618 Msa0001460:intron 40.0%
!! ATTCCCTAAATTTGCTGGCT+GGG + chr1_1:3157600-3157619 Msa0001460:intron 40.0%
AATCTCATGTGGGAGACAAA+AGG - chr1_1:3157662-3157681 None:intergenic 40.0%
CATATCATTGTGTATGGCAG+TGG + chr1_1:3157716-3157735 Msa0001460:intron 40.0%
GGTGCAGTCAACTCAATAAT+TGG + chr1_1:3157811-3157830 Msa0001460:intron 40.0%
AGTCAACTCAATAATTGGCG+CGG + chr1_1:3157816-3157835 Msa0001460:intron 40.0%
ATAATCAGCTGCTGTGTAAG+TGG + chr1_1:3158319-3158338 Msa0001460:intron 40.0%
TAATCAGCTGCTGTGTAAGT+GGG + chr1_1:3158320-3158339 Msa0001460:intron 40.0%
ATCACGGCTTACTAGTATGA+AGG + chr1_1:3158701-3158720 Msa0001460:CDS 40.0%
CATATTCACTTGGATCACGT+GGG - chr1_1:3158973-3158992 None:intergenic 40.0%
ACATATTCACTTGGATCACG+TGG - chr1_1:3158974-3158993 None:intergenic 40.0%
TCTCAATACTCATAAGCCGA+AGG - chr1_1:3159130-3159149 None:intergenic 40.0%
GTATTGAGAGCTGAAGAAGT+AGG + chr1_1:3159141-3159160 Msa0001460:exon 40.0%
AAGTCTAACATGCATGGCAA+AGG + chr1_1:3159228-3159247 Msa0001460:three_prime_UTR 40.0%
! GGCAAAGGAAGGAAATCTTT+TGG + chr1_1:3159243-3159262 Msa0001460:three_prime_UTR 40.0%
TGATTGCCAGCATCTTTAGA+TGG + chr1_1:3159338-3159357 Msa0001460:three_prime_UTR 40.0%
CAAAGACCATCTAAAGATGC+TGG - chr1_1:3159347-3159366 None:intergenic 40.0%
CAGCATCTTTAGATGGTCTT+TGG + chr1_1:3159345-3159364 Msa0001460:three_prime_UTR 40.0%
!!! TTTTGCAGGAGTGTCATTTC+CGG - chr1_1:3159431-3159450 None:intergenic 40.0%
GAAGAGAATCAAAATGGCGG+TGG + chr1_1:3156313-3156332 Msa0001460:exon 45.0%
!! TTGCTGTTTAGCATCGGAGT+TGG + chr1_1:3156362-3156381 Msa0001460:CDS 45.0%
ACTGCAACTCATTCGAGAGT+TGG + chr1_1:3156596-3156615 Msa0001460:CDS 45.0%
CAACTCATTCGAGAGTTGGA+TGG + chr1_1:3156600-3156619 Msa0001460:CDS 45.0%
ACTCATTCGAGAGTTGGATG+GGG + chr1_1:3156602-3156621 Msa0001460:CDS 45.0%
AGTTGGATGGGGAGACAATA+GGG + chr1_1:3156613-3156632 Msa0001460:CDS 45.0%
TAGCAGTCAATCATTGGCAC+GGG + chr1_1:3156637-3156656 Msa0001460:CDS 45.0%
GGCGAGTTAAAGAGAGGTTA+TGG - chr1_1:3156739-3156758 None:intergenic 45.0%
ACATGGCCTGTATGTTGGAA+GGG - chr1_1:3156760-3156779 None:intergenic 45.0%
CAAGACTTTCCGGCAATAAC+AGG - chr1_1:3156791-3156810 None:intergenic 45.0%
ATGTCTTTCCAGAACCACTC+TGG - chr1_1:3156826-3156845 None:intergenic 45.0%
GGTGCGAGTATAACAAGAGT+AGG - chr1_1:3156926-3156945 None:intergenic 45.0%
TGAGAAGCTAACTCAGCTGT+GGG - chr1_1:3156947-3156966 None:intergenic 45.0%
CTATGAAGACATCAACACCC+TGG - chr1_1:3157075-3157094 None:intergenic 45.0%
GATGTCTTCATAGCTACACC+TGG + chr1_1:3157082-3157101 Msa0001460:CDS 45.0%
! GCAAAAAGCCTTGGTTGATG+AGG - chr1_1:3157120-3157139 None:intergenic 45.0%
TGATATCCTCTTTGGCGATG+AGG + chr1_1:3157335-3157354 Msa0001460:CDS 45.0%
! CTTGATTAGTTCTTCACCGG+TGG + chr1_1:3157380-3157399 Msa0001460:CDS 45.0%
CCCATGACCATTTCACAATC+AGG - chr1_1:3157474-3157493 None:intergenic 45.0%
AGTGAAACCTGACCTCTTCA+AGG - chr1_1:3157524-3157543 None:intergenic 45.0%
AAGCCCAGCCAGCAAATTTA+GGG - chr1_1:3157606-3157625 None:intergenic 45.0%
ACCTGTCTCTGTTGTCAATG+CGG + chr1_1:3157777-3157796 Msa0001460:intron 45.0%
CTGTTGTCAATGCGGAAATG+CGG + chr1_1:3157785-3157804 Msa0001460:intron 45.0%
AAAGTTGAAAGCGGCCAAAG+CGG - chr1_1:3158054-3158073 None:intergenic 45.0%
CGCGTGTCAAAAGTTGAAAG+CGG - chr1_1:3158063-3158082 None:intergenic 45.0%
! GTAGATTGTAGTGGGGAAGA+TGG + chr1_1:3158377-3158396 Msa0001460:CDS 45.0%
ATTCTTGTGTCATGGCAGCA+TGG - chr1_1:3158683-3158702 None:intergenic 45.0%
CTGCCATGACACAAGAATCA+CGG + chr1_1:3158685-3158704 Msa0001460:CDS 45.0%
AAGCCGTGATTCTTGTGTCA+TGG - chr1_1:3158691-3158710 None:intergenic 45.0%
! AACTGGGACACTTCTTTTGG+TGG - chr1_1:3158741-3158760 None:intergenic 45.0%
! GTCCCAGTTTATGGTTTGCA+CGG + chr1_1:3158752-3158771 Msa0001460:CDS 45.0%
GTCCGTGCAAACCATAAACT+GGG - chr1_1:3158757-3158776 None:intergenic 45.0%
TGTCCGTGCAAACCATAAAC+TGG - chr1_1:3158758-3158777 None:intergenic 45.0%
! AGTTTATGGTTTGCACGGAC+AGG + chr1_1:3158757-3158776 Msa0001460:CDS 45.0%
CAACTTGTAACAGAGCTTCG+CGG + chr1_1:3158903-3158922 Msa0001460:intron 45.0%
AACTTGTAACAGAGCTTCGC+GGG + chr1_1:3158904-3158923 Msa0001460:intron 45.0%
! GGGAATTGACTTCACTAGAG+TGG + chr1_1:3158925-3158944 Msa0001460:CDS 45.0%
ACACGCCGTACATATTCACT+TGG - chr1_1:3158983-3159002 None:intergenic 45.0%
AGTGAATATGTACGGCGTGT+TGG + chr1_1:3158983-3159002 Msa0001460:CDS 45.0%
TCCACTGCATGATGTACCTT+CGG + chr1_1:3159111-3159130 Msa0001460:CDS 45.0%
GAGCTGAAGAAGTAGGAAGT+TGG + chr1_1:3159148-3159167 Msa0001460:three_prime_UTR 45.0%
CTAACATGCATGGCAAAGGA+AGG + chr1_1:3159232-3159251 Msa0001460:three_prime_UTR 45.0%
!! GGTAGCACCGACAATATTCT+AGG + chr1_1:3159264-3159283 Msa0001460:three_prime_UTR 45.0%
TCGATGAAACGTGCATTGCT+AGG + chr1_1:3159287-3159306 Msa0001460:three_prime_UTR 45.0%
GCTCCTCATAGCAAAAGCAA+GGG + chr1_1:3159309-3159328 Msa0001460:three_prime_UTR 45.0%
!! TAGCCCTTGCTTTTGCTATG+AGG - chr1_1:3159315-3159334 None:intergenic 45.0%
TCTTTAGATGGTCTTTGGGC+AGG + chr1_1:3159350-3159369 Msa0001460:three_prime_UTR 45.0%
!!! ACTCGTGGTTTTATCGACGG+CGG - chr1_1:3156456-3156475 None:intergenic 50.0%
GAGTTGGATGGGGAGACAAT+AGG + chr1_1:3156612-3156631 Msa0001460:CDS 50.0%
TCAATCATTGGCACGGGCAT+CGG + chr1_1:3156643-3156662 Msa0001460:CDS 50.0%
TGGAAGGGCGAGTTAAAGAG+AGG - chr1_1:3156745-3156764 None:intergenic 50.0%
AACTCGCCCTTCCAACATAC+AGG + chr1_1:3156751-3156770 Msa0001460:CDS 50.0%
GACATGGCCTGTATGTTGGA+AGG - chr1_1:3156761-3156780 None:intergenic 50.0%
ATTGCTGACCAGAGTGGTTC+TGG + chr1_1:3156815-3156834 Msa0001460:CDS 50.0%
CTGAGAAGCTAACTCAGCTG+TGG - chr1_1:3156948-3156967 None:intergenic 50.0%
! CACAGCTGAGTTAGCTTCTC+AGG + chr1_1:3156946-3156965 Msa0001460:CDS 50.0%
ATGAGGAAGAGAAAACGGCC+AGG - chr1_1:3157103-3157122 None:intergenic 50.0%
TTGCAGTGCTGTCTTGGATG+AGG + chr1_1:3157311-3157330 Msa0001460:intron 50.0%
TGGCGATGAGGACTTCGAAA+AGG + chr1_1:3157347-3157366 Msa0001460:CDS 50.0%
CAAGCCCAGCCAGCAAATTT+AGG - chr1_1:3157607-3157626 None:intergenic 50.0%
TATGGCAGTGGCAGTACAGT+TGG + chr1_1:3157728-3157747 Msa0001460:intron 50.0%
! TCCGCATTGACAACAGAGAC+AGG - chr1_1:3157781-3157800 None:intergenic 50.0%
ACTCAATAATTGGCGCGGTG+CGG + chr1_1:3157821-3157840 Msa0001460:intron 50.0%
AAAAACAGGACCACCGCACT+AGG - chr1_1:3158115-3158134 None:intergenic 50.0%
!!! TGGGGTATTTTGGTGTGTGC+AGG + chr1_1:3158211-3158230 Msa0001460:intron 50.0%
ACTGGTCACTGAATCCAGCA+TGG - chr1_1:3158237-3158256 None:intergenic 50.0%
TGTGTCATGGCAGCATGGAA+AGG - chr1_1:3158678-3158697 None:intergenic 50.0%
ACTTGTAACAGAGCTTCGCG+GGG + chr1_1:3158905-3158924 Msa0001460:intron 50.0%
!! TTGGAAAGGGTGCTCTCAAC+TGG + chr1_1:3159185-3159204 Msa0001460:three_prime_UTR 50.0%
!! TGGAAAGGGTGCTCTCAACT+GGG + chr1_1:3159186-3159205 Msa0001460:three_prime_UTR 50.0%
GGCTCCTCATAGCAAAAGCA+AGG + chr1_1:3159308-3159327 Msa0001460:three_prime_UTR 50.0%
AAAATGGCGGTGGTTGCGCA+AGG + chr1_1:3156323-3156342 Msa0001460:exon 55.0%
! GTGGTTGCGCAAGGTGTTTG+TGG + chr1_1:3156332-3156351 Msa0001460:CDS 55.0%
CGAAGTCCTCATCGCCAAAG+AGG - chr1_1:3157344-3157363 None:intergenic 55.0%
GTTCTCGCCTTGAAGAGGTC+AGG + chr1_1:3157514-3157533 Msa0001460:intron 55.0%
CTCAATAATTGGCGCGGTGC+GGG + chr1_1:3157822-3157841 Msa0001460:intron 55.0%
! GGGGCACTATTTCGGTAGTG+CGG + chr1_1:3157848-3157867 Msa0001460:intron 55.0%
!! ACTTTTGACACGCGCTGACG+CGG + chr1_1:3158069-3158088 Msa0001460:intron 55.0%
CGGAGTGGAGCGGAAAAAAC+AGG - chr1_1:3158129-3158148 None:intergenic 55.0%
AGCTGCTCTTCTGCAGCTTG+CGG + chr1_1:3158439-3158458 Msa0001460:CDS 55.0%
CGTGTTGGAAGAACAGCCAG+AGG + chr1_1:3158998-3159017 Msa0001460:CDS 55.0%
!! AAGAACAGCCAGAGGTGCCA+GGG + chr1_1:3159006-3159025 Msa0001460:CDS 55.0%
! GCCGAAGGTACATCATGCAG+TGG - chr1_1:3159115-3159134 None:intergenic 55.0%
!! TGCAGGAGTGTCATTTCCGG+TGG - chr1_1:3159428-3159447 None:intergenic 55.0%
!! ATCATTGGCACGGGCATCGG+AGG + chr1_1:3156646-3156665 Msa0001460:CDS 60.0%
GTCAATGCGGAAATGCGGCG+CGG + chr1_1:3157790-3157809 Msa0001460:intron 60.0%
TCCGCTCCACTCCGCTATAG+CGG + chr1_1:3158135-3158154 Msa0001460:intron 60.0%
! ACCGCTATAGCGGAGTGGAG+CGG - chr1_1:3158139-3158158 None:intergenic 60.0%
AGCCACGTCGACCGCTATAG+CGG - chr1_1:3158149-3158168 None:intergenic 60.0%
TTGGTGTGTGCAGGCCATGC+TGG + chr1_1:3158220-3158239 Msa0001460:intron 60.0%
!! GAAGAACAGCCAGAGGTGCC+AGG + chr1_1:3159005-3159024 Msa0001460:CDS 60.0%
!! AGAACAGCCAGAGGTGCCAG+GGG + chr1_1:3159007-3159026 Msa0001460:CDS 60.0%
AAATGCCTTGCCCTGTCCCC+TGG - chr1_1:3159026-3159045 None:intergenic 60.0%
TAATTGGCGCGGTGCGGGAG+CGG + chr1_1:3157827-3157846 Msa0001460:intron 65.0%
! GCGGGAGCGGGGCACTATTT+CGG + chr1_1:3157840-3157859 Msa0001460:intron 65.0%
! CGTCGACCGCTATAGCGGAG+TGG - chr1_1:3158144-3158163 None:intergenic 65.0%
CTCCGCTATAGCGGTCGACG+TGG + chr1_1:3158144-3158163 Msa0001460:intron 65.0%
AATTGGCGCGGTGCGGGAGC+GGG + chr1_1:3157828-3157847 Msa0001460:intron 70.0%
ACCACCGCACTAGGCCGAGC+CGG - chr1_1:3158106-3158125 None:intergenic 70.0%
!! AGCCAGAGGTGCCAGGGGAC+AGG + chr1_1:3159012-3159031 Msa0001460:CDS 70.0%
!! GCCAGAGGTGCCAGGGGACA+GGG + chr1_1:3159013-3159032 Msa0001460:CDS 70.0%
AGGTGCCAGGGGACAGGGCA+AGG + chr1_1:3159018-3159037 Msa0001460:CDS 70.0%
ATTGGCGCGGTGCGGGAGCG+GGG + chr1_1:3157829-3157848 Msa0001460:intron 75.0%
TGACGCGGCCTGAGACGCGC+CGG + chr1_1:3158084-3158103 Msa0001460:intron 75.0%
GCCCTGTCCCCTGGCACCTC+TGG - chr1_1:3159017-3159036 None:intergenic 75.0%
!!! CGGCCTGAGACGCGCCGGCT+CGG + chr1_1:3158089-3158108 Msa0001460:intron 80.0%
! AGGCCGAGCCGGCGCGTCTC+AGG - chr1_1:3158095-3158114 None:intergenic 80.0%
!!! CGCGCCGGCTCGGCCTAGTG+CGG + chr1_1:3158099-3158118 Msa0001460:intron 80.0%
!!! GCCGGCTCGGCCTAGTGCGG+TGG + chr1_1:3158102-3158121 Msa0001460:intron 80.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 3156266 3159560 3156266 ID=Msa0001460;Name=Msa0001460
chr1_1 mRNA 3156266 3159560 3156266 ID=Msa0001460-mRNA-1;Parent=Msa0001460;Name=Msa0001460-mRNA-1;_AED=0.08;_eAED=0.08;_QI=60|1|1|1|1|1|5|413|528
chr1_1 exon 3156266 3157158 3156266 ID=Msa0001460-mRNA-1:exon:742;Parent=Msa0001460-mRNA-1
chr1_1 exon 3157317 3157530 3157317 ID=Msa0001460-mRNA-1:exon:743;Parent=Msa0001460-mRNA-1
chr1_1 exon 3158371 3158502 3158371 ID=Msa0001460-mRNA-1:exon:744;Parent=Msa0001460-mRNA-1
chr1_1 exon 3158603 3158778 3158603 ID=Msa0001460-mRNA-1:exon:745;Parent=Msa0001460-mRNA-1
chr1_1 exon 3158916 3159560 3158916 ID=Msa0001460-mRNA-1:exon:746;Parent=Msa0001460-mRNA-1
chr1_1 five_prime_UTR 3156266 3156325 3156266 ID=Msa0001460-mRNA-1:five_prime_utr;Parent=Msa0001460-mRNA-1
chr1_1 CDS 3156326 3157158 3156326 ID=Msa0001460-mRNA-1:cds;Parent=Msa0001460-mRNA-1
chr1_1 CDS 3157317 3157530 3157317 ID=Msa0001460-mRNA-1:cds;Parent=Msa0001460-mRNA-1
chr1_1 CDS 3158371 3158502 3158371 ID=Msa0001460-mRNA-1:cds;Parent=Msa0001460-mRNA-1
chr1_1 CDS 3158603 3158778 3158603 ID=Msa0001460-mRNA-1:cds;Parent=Msa0001460-mRNA-1
chr1_1 CDS 3158916 3159147 3158916 ID=Msa0001460-mRNA-1:cds;Parent=Msa0001460-mRNA-1
chr1_1 three_prime_UTR 3159148 3159560 3159148 ID=Msa0001460-mRNA-1:three_prime_utr;Parent=Msa0001460-mRNA-1
Gene Sequence

>Msa0001460

ATGGCGGTGGTTGCGCAAGGTGTTTGTGGTAAAGCGTTGCTGTTTAGCATCGGAGTTGGAGTGAGACCCAGTAAACACTCTGTCTTCTTCTTCGTTTCTAATTCAAAATCCAATTCAAATTCAAAATCCGCCGTCGATAAAACCACGAGTTTCGGAAGACTAAAAGTTGAGAAAGTGAATGCTCTCATCCACAGAACCAAGCAAAATACCACAAAACGCAAACAACTACAACTAGAAGATGAAGATGAAGACAACAATTCTGCAACTGCAACTGCAACTCATTCGAGAGTTGGATGGGGAGACAATAGGGTTAGCAGTCAATCATTGGCACGGGCATCGGAGGACACCAACTTTTTCAGTCTAAAATCATTTAAAGACATTGGATGCGCTGATTTCATGATTGAATCTCTCCATAACCTCTCTTTAACTCGCCCTTCCAACATACAGGCCATGTCATTTGCTCCTGTTATTGCCGGAAAGTCTTGTATAATTGCTGACCAGAGTGGTTCTGGAAAGACATTAGCTTATCTTGTACCAATCATTCAACGTCTCAGACAACAAGAACTACAAGGACTTCATAAATCTTCTTCACGATCTCCTACTCTTGTTATACTCGCACCCACAGCTGAGTTAGCTTCTCAGGTCTTCCATAATTGTCGTTCAATGTCTAAATCTGGCAAGGTTCCATTCAAATCTATGCTTGTCACTGGTGGCTTCCGACAAAAAACTCAACTCGACACTTTAAACCAGGGTGTTGATGTCTTCATAGCTACACCTGGCCGTTTTCTCTTCCTCATCAACCAAGGCTTTTTGCACTTAACTAATCTAACATGTGCTGTCTTGGATGAGGTTGATATCCTCTTTGGCGATGAGGACTTCGAAAAGGCTCTTCAATGCTTGATTAGTTCTTCACCGGTGGACACACAATATTTATTTGTAACTGCTACTTTACCAAAAAATGTTTACAGCAAACTTGTTGAAGTTTTTCCTGATTGTGAAATGGTCATGGGACCTAGTATGCATCGAATAAGTTCTCGCCTTGAAGAGATAATAGTAGATTGTAGTGGGGAAGATGGACAAGAAAAAACTCTCGACACAGCATTTTTGAACAAGAAAGCTGCTCTTCTGCAGCTTGCGGAGAAGAATCGCGTCCCAAGAACTATTGTGTTCTGCAACAAAATTGAAACATGCAGAAAAGTTGAAAATGCATTAAACCGTTTTGATAGAAAGGGAATTCGCATGCAAGTTCTACCTTTCCATGCTGCCATGACACAAGAATCACGGCTTACTAGTATGAAGGAGTTTACACGTTCTCCACCAAAAGAAGTGTCCCAGTTTATGGTTTGCACGGACAGAGCTTCGCGGGGAATTGACTTCACTAGAGTGGATCATGTAATACTTTTTGACTACCCACGTGATCCAAGTGAATATGTACGGCGTGTTGGAAGAACAGCCAGAGGTGCCAGGGGACAGGGCAAGGCATTTATCTTTGTTGTTGGCAAGCAAGTATCCCTTGCACAAAAAGTAATGGAAAGAAATCGGAAGGGTCATCCACTGCATGATGTACCTTCGGCTTATGAGTATTGA
Protein sequence

>Msa0001460

MAVVAQGVCGKALLFSIGVGVRPSKHSVFFFVSNSKSNSNSKSAVDKTTSFGRLKVEKVNALIHRTKQNTTKRKQLQLEDEDEDNNSATATATHSRVGWGDNRVSSQSLARASEDTNFFSLKSFKDIGCADFMIESLHNLSLTRPSNIQAMSFAPVIAGKSCIIADQSGSGKTLAYLVPIIQRLRQQELQGLHKSSSRSPTLVILAPTAELASQVFHNCRSMSKSGKVPFKSMLVTGGFRQKTQLDTLNQGVDVFIATPGRFLFLINQGFLHLTNLTCAVLDEVDILFGDEDFEKALQCLISSSPVDTQYLFVTATLPKNVYSKLVEVFPDCEMVMGPSMHRISSRLEEIIVDCSGEDGQEKTLDTAFLNKKAALLQLAEKNRVPRTIVFCNKIETCRKVENALNRFDRKGIRMQVLPFHAAMTQESRLTSMKEFTRSPPKEVSQFMVCTDRASRGIDFTRVDHVILFDYPRDPSEYVRRVGRTARGARGQGKAFIFVVGKQVSLAQKVMERNRKGHPLHDVPSAYEY*