AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1454320


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1454320 MtrunA17_Chr7g0273741 84.615 65 10 0 1 65 1 65 2.41e-33 114
Msa1454320 MtrunA17_Chr7g0273781 87.692 65 8 0 1 65 34 98 5.89e-33 120
Msa1454320 MtrunA17_Chr7g0273721 75.309 81 16 2 1 77 1 81 1.10e-32 119
Msa1454320 MtrunA17_Chr7g0273791 87.692 65 8 0 1 65 1 65 1.31e-32 119
Msa1454320 MtrunA17_Chr5g0433641 87.692 65 8 0 1 65 1 65 1.91e-32 118
Msa1454320 MtrunA17_Chr7g0273731 83.077 65 11 0 1 65 84 148 2.18e-30 113
Msa1454320 MtrunA17_Chr7g0273761 81.818 66 11 1 1 65 372 437 2.93e-29 110
Msa1454320 MtrunA17_Chr3g0098961 72.308 65 18 0 1 65 4 68 3.64e-27 99.4
Msa1454320 MtrunA17_Chr7g0273671 95.349 43 2 0 1 43 1 43 2.27e-23 85.5
Msa1454320 MtrunA17_Chr8g0353421 63.333 60 22 0 3 62 2 61 2.45e-23 88.2
Msa1454320 MtrunA17_Chr1g0165671 64.516 62 22 0 1 62 18 79 1.65e-22 91.7
Msa1454320 MtrunA17_Chr1g0165711 65.000 60 21 0 3 62 2 61 1.05e-21 89.0
Msa1454320 MtrunA17_Chr1g0191081 65.574 61 21 0 3 63 11 71 1.42e-20 85.9
Msa1454320 MtrunA17_Chr1g0165681 42.857 98 55 1 3 100 2 98 4.06e-19 82.0
Msa1454320 MtrunA17_Chr1g0165741 56.667 60 26 0 3 62 2 61 6.20e-19 77.4
Msa1454320 MtrunA17_Chr1g0165661 56.667 60 26 0 3 62 2 61 1.14e-18 80.5
Msa1454320 MtrunA17_Chr6g0471371 55.000 60 27 0 3 62 2 61 1.37e-18 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1454320 AT5G17540.1 63.333 60 21 1 3 62 2 60 1.48e-18 80.9
Msa1454320 AT3G03480.1 59.677 62 24 1 2 63 11 71 2.92e-15 71.2

Find 38 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATGTCTAAGAACCTTAAT+AGG 0.234481 tig0035128:-16331 None:intergenic
GGCTGTGCTGACAAATTTGC+AGG 0.312819 tig0035128:-16609 None:intergenic
TTGCCCCTGGCAACTTTGCC+AGG 0.318050 tig0035128:-16462 None:intergenic
GGGTCTTTCTCTGCCATTGA+TGG 0.353609 tig0035128:-16310 None:intergenic
GCTATTCACGTAGCCTTTGC+AGG 0.360218 tig0035128:-16630 None:intergenic
GAAAAGATTCTTGCGTTGTC+AGG 0.384539 tig0035128:+16520 Msa1454320:CDS
GGCAGAGAAAGACCCTATTA+AGG 0.385270 tig0035128:+16318 Msa1454320:CDS
TGCAGGCTTTGCAATTTGCC+AGG 0.402403 tig0035128:-16592 None:intergenic
AAACTCCTGGCAAAGTTGCC+AGG 0.422701 tig0035128:+16457 Msa1454320:CDS
AAAAGCCGTGGCAAAATGTA+TGG 0.439383 tig0035128:+16493 Msa1454320:CDS
TCATGAGGAGTGGGTGCGGC+TGG 0.441956 tig0035128:-16208 None:intergenic
GGATGGTACAACACTTTGCC+AGG 0.461267 tig0035128:+16550 Msa1454320:CDS
CATGTCTAAGAACCTTAATA+GGG 0.477028 tig0035128:-16330 None:intergenic
CTATCGTCACGAACCATCAA+TGG 0.495351 tig0035128:+16297 Msa1454320:CDS
GCGGCTGGAAGCACCAACTC+TGG 0.506946 tig0035128:-16193 None:intergenic
GCAGGCTTTGCAATTTGCCA+GGG 0.519971 tig0035128:-16591 None:intergenic
AACTTCATGAGGAGTGGGTG+CGG 0.526875 tig0035128:-16212 None:intergenic
AGTTTAACTTCATGAGGAGT+GGG 0.527780 tig0035128:-16217 None:intergenic
CTTGCGTTGTCAGGAAAAGC+AGG 0.531543 tig0035128:+16529 Msa1454320:CDS
AACTCCTGGCAAAGTTGCCA+GGG 0.537313 tig0035128:+16458 Msa1454320:CDS
AATTTGCCAGGAGCTATGCC+AGG 0.547193 tig0035128:+16432 Msa1454320:intron
TCTGACATTGATGATCAAGA+AGG 0.548394 tig0035128:+16244 Msa1454320:CDS
CAGGCTTTGCAATTTGCCAG+GGG 0.549060 tig0035128:-16590 None:intergenic
GCGGAGGTGCCAACCAGAGT+TGG 0.552570 tig0035128:+16180 Msa1454320:CDS
CTGGAAGCACCAACTCTGGT+TGG 0.561668 tig0035128:-16189 None:intergenic
GCTATGCCAGGGAAAACTCC+TGG 0.564673 tig0035128:+16444 Msa1454320:CDS
GAGTTTAACTTCATGAGGAG+TGG 0.565045 tig0035128:-16218 None:intergenic
GAGAGGAGTTTAACTTCATG+AGG 0.572367 tig0035128:-16223 None:intergenic
TGACGATAGATAAATATGAC+AGG 0.579372 tig0035128:-16283 None:intergenic
TTTGTCAGCACAGCCTGCAA+AGG 0.615733 tig0035128:+16617 Msa1454320:CDS
GATGGTACAACACTTTGCCA+GGG 0.624520 tig0035128:+16551 Msa1454320:CDS
CGTTGTCAGGAAAAGCAGGA+TGG 0.629679 tig0035128:+16533 Msa1454320:CDS
GCAACCCCTCACAAAAGCCG+TGG 0.633905 tig0035128:+16481 Msa1454320:CDS
ATGGTACAACACTTTGCCAG+GGG 0.671894 tig0035128:+16552 Msa1454320:CDS
ATTTGCCAGGAGCTATGCCA+GGG 0.676291 tig0035128:+16433 Msa1454320:intron
CTTGATCATCAATGTCAGAG+AGG 0.682333 tig0035128:-16240 None:intergenic
ACTCCTGGCAAAGTTGCCAG+GGG 0.709398 tig0035128:+16459 Msa1454320:CDS
CATCGTTAATGTTCACAGTG+CGG 0.731695 tig0035128:+16161 Msa1454320:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TGACGATAGATAAATATGAC+AGG - tig0035128:16286-16305 None:intergenic 30.0%
CATGTCTAAGAACCTTAATA+GGG - tig0035128:16333-16352 None:intergenic 30.0%
! AGTTTAACTTCATGAGGAGT+GGG - tig0035128:16220-16239 None:intergenic 35.0%
! TCTGACATTGATGATCAAGA+AGG + tig0035128:16244-16263 Msa1454320:CDS 35.0%
GCATGTCTAAGAACCTTAAT+AGG - tig0035128:16334-16353 None:intergenic 35.0%
AAGAAATCTTGCATATTGCC+AGG + tig0035128:16379-16398 Msa1454320:intron 35.0%
AGAAATCTTGCATATTGCCA+GGG + tig0035128:16380-16399 Msa1454320:intron 35.0%
CATCGTTAATGTTCACAGTG+CGG + tig0035128:16161-16180 Msa1454320:CDS 40.0%
! GAGTTTAACTTCATGAGGAG+TGG - tig0035128:16221-16240 None:intergenic 40.0%
! GAGAGGAGTTTAACTTCATG+AGG - tig0035128:16226-16245 None:intergenic 40.0%
CTTGATCATCAATGTCAGAG+AGG - tig0035128:16243-16262 None:intergenic 40.0%
AAAAGCCGTGGCAAAATGTA+TGG + tig0035128:16493-16512 Msa1454320:CDS 40.0%
! CTGATCCATACATTTTGCCA+CGG - tig0035128:16501-16520 None:intergenic 40.0%
GAAAAGATTCTTGCGTTGTC+AGG + tig0035128:16520-16539 Msa1454320:CDS 40.0%
!!! AATTTGCCAGGGGTTTTTTG+TGG - tig0035128:16583-16602 None:intergenic 40.0%
CTATCGTCACGAACCATCAA+TGG + tig0035128:16297-16316 Msa1454320:CDS 45.0%
GGCAGAGAAAGACCCTATTA+AGG + tig0035128:16318-16337 Msa1454320:CDS 45.0%
! TTTTGCCACGGCTTTTGTGA+GGG - tig0035128:16489-16508 None:intergenic 45.0%
! ATTTTGCCACGGCTTTTGTG+AGG - tig0035128:16490-16509 None:intergenic 45.0%
GATGGTACAACACTTTGCCA+GGG + tig0035128:16551-16570 Msa1454320:CDS 45.0%
ATGGTACAACACTTTGCCAG+GGG + tig0035128:16552-16571 Msa1454320:CDS 45.0%
CGTTAATGTTCACAGTGCGG+AGG + tig0035128:16164-16183 Msa1454320:CDS 50.0%
AACTTCATGAGGAGTGGGTG+CGG - tig0035128:16215-16234 None:intergenic 50.0%
GGGTCTTTCTCTGCCATTGA+TGG - tig0035128:16313-16332 None:intergenic 50.0%
TATTGCCAGGGAACAATGCC+AGG + tig0035128:16392-16411 Msa1454320:intron 50.0%
GTAGTCCTGGCAAATTTGCC+AGG + tig0035128:16420-16439 Msa1454320:intron 50.0%
TAGCTCCTGGCAAATTTGCC+AGG - tig0035128:16428-16447 None:intergenic 50.0%
AATTTGCCAGGAGCTATGCC+AGG + tig0035128:16432-16451 Msa1454320:intron 50.0%
ATTTGCCAGGAGCTATGCCA+GGG + tig0035128:16433-16452 Msa1454320:intron 50.0%
! ACTTTGCCAGGAGTTTTCCC+TGG - tig0035128:16453-16472 None:intergenic 50.0%
AAACTCCTGGCAAAGTTGCC+AGG + tig0035128:16457-16476 Msa1454320:CDS 50.0%
AACTCCTGGCAAAGTTGCCA+GGG + tig0035128:16458-16477 Msa1454320:CDS 50.0%
! TTTGCCACGGCTTTTGTGAG+GGG - tig0035128:16488-16507 None:intergenic 50.0%
CTTGCGTTGTCAGGAAAAGC+AGG + tig0035128:16529-16548 Msa1454320:CDS 50.0%
CGTTGTCAGGAAAAGCAGGA+TGG + tig0035128:16533-16552 Msa1454320:CDS 50.0%
GGATGGTACAACACTTTGCC+AGG + tig0035128:16550-16569 Msa1454320:CDS 50.0%
!!! GTTTTTTGTGGCACAACCCC+TGG - tig0035128:16571-16590 None:intergenic 50.0%
GTTGTGCCACAAAAAACCCC+TGG + tig0035128:16574-16593 Msa1454320:CDS 50.0%
CAGGCTTTGCAATTTGCCAG+GGG - tig0035128:16593-16612 None:intergenic 50.0%
GCAGGCTTTGCAATTTGCCA+GGG - tig0035128:16594-16613 None:intergenic 50.0%
TGCAGGCTTTGCAATTTGCC+AGG - tig0035128:16595-16614 None:intergenic 50.0%
! GGCTGTGCTGACAAATTTGC+AGG - tig0035128:16612-16631 None:intergenic 50.0%
TTTGTCAGCACAGCCTGCAA+AGG + tig0035128:16617-16636 Msa1454320:CDS 50.0%
!! CTGGAAGCACCAACTCTGGT+TGG - tig0035128:16192-16211 None:intergenic 55.0%
TTGCTCCTGGCATTGTTCCC+TGG - tig0035128:16400-16419 None:intergenic 55.0%
ATGCCAGGAGCAAGTAGTCC+TGG + tig0035128:16407-16426 Msa1454320:intron 55.0%
TTGCCAGGACTACTTGCTCC+TGG - tig0035128:16413-16432 None:intergenic 55.0%
! GTTTTCCCTGGCATAGCTCC+TGG - tig0035128:16441-16460 None:intergenic 55.0%
GCTATGCCAGGGAAAACTCC+TGG + tig0035128:16444-16463 Msa1454320:CDS 55.0%
ACTCCTGGCAAAGTTGCCAG+GGG + tig0035128:16459-16478 Msa1454320:CDS 55.0%
TTGCCCCTGGCAACTTTGCC+AGG - tig0035128:16465-16484 None:intergenic 60.0%
! CTTTTGTGAGGGGTTGCCCC+TGG - tig0035128:16478-16497 None:intergenic 60.0%
GCAACCCCTCACAAAAGCCG+TGG + tig0035128:16481-16500 Msa1454320:CDS 60.0%
!! GCGGAGGTGCCAACCAGAGT+TGG + tig0035128:16180-16199 Msa1454320:CDS 65.0%
!! GCGGCTGGAAGCACCAACTC+TGG - tig0035128:16196-16215 None:intergenic 65.0%
TCATGAGGAGTGGGTGCGGC+TGG - tig0035128:16211-16230 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0035128 gene 16151 16651 16151 ID=Msa1454320;Name=Msa1454320
tig0035128 mRNA 16151 16651 16151 ID=Msa1454320-mRNA-1;Parent=Msa1454320;Name=Msa1454320-mRNA-1;_AED=0.71;_eAED=0.71;_QI=0|0|0|0.5|1|1|2|0|132
tig0035128 exon 16151 16339 16151 ID=Msa1454320-mRNA-1:exon:19658;Parent=Msa1454320-mRNA-1
tig0035128 exon 16442 16651 16442 ID=Msa1454320-mRNA-1:exon:19659;Parent=Msa1454320-mRNA-1
tig0035128 CDS 16151 16339 16151 ID=Msa1454320-mRNA-1:cds;Parent=Msa1454320-mRNA-1
tig0035128 CDS 16442 16651 16442 ID=Msa1454320-mRNA-1:cds;Parent=Msa1454320-mRNA-1
Gene Sequence

>Msa1454320

ATGACTTCATCATCGTTAATGTTCACAGTGCGGAGGTGCCAACCAGAGTTGGTGCTTCCAGCCGCACCCACTCCTCATGAAGTTAAACTCCTCTCTGACATTGATGATCAAGAAGGTTTGCGTTTCAATATTCCTGTCATATTTATCTATCGTCACGAACCATCAATGGCAGAGAAAGACCCTATTAAGGAGCTATGCCAGGGAAAACTCCTGGCAAAGTTGCCAGGGGCAACCCCTCACAAAAGCCGTGGCAAAATGTATGGATCAGAAAAGATTCTTGCGTTGTCAGGAAAAGCAGGATGGTACAACACTTTGCCAGGGGTTGTGCCACAAAAAACCCCTGGCAAATTGCAAAGCCTGCAAATTTGTCAGCACAGCCTGCAAAGGCTACGTGAATAG

Protein sequence

>Msa1454320

MTSSSLMFTVRRCQPELVLPAAPTPHEVKLLSDIDDQEGLRFNIPVIFIYRHEPSMAEKDPIKELCQGKLLAKLPGATPHKSRGKMYGSEKILALSGKAGWYNTLPGVVPQKTPGKLQSLQICQHSLQRLRE*