AlfalfaGEDB | Msa0600500

M. sativa cultivar zhongmu-4 / Msa0600500

KEGG: -
GO: -
PFAM: PF08100.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0600500	XP_003605744.1	99.403	335	2	0	1	335	35	369	0.0	696

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0600500	sp\|Q6WUC2\|7OMT_PAPSO	50.000	340	161	5	1	334	18	354	2.17e-120	353

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0600500	G7JTH4	99.403	335	2	0	1	335	35	369	0.0	696

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa0600500	0.878728	2.220091e-69	-8.615850e-47
Msa0001040	Msa0600500	0.800045	1.732722e-48	-8.615850e-47
Msa0002110	Msa0600500	0.844893	5.701757e-59	-8.615850e-47
Msa0002690	Msa0600500	0.858117	1.026490e-62	-8.615850e-47
Msa0002700	Msa0600500	0.860856	1.542208e-63	-8.615850e-47
Msa0003340	Msa0600500	0.822942	1.808468e-53	-8.615850e-47
Msa0004180	Msa0600500	0.822495	2.297549e-53	-8.615850e-47
Msa0005500	Msa0600500	0.841079	5.904999e-58	-8.615850e-47
Msa0007570	Msa0600500	0.826873	2.136616e-54	-8.615850e-47
Msa0007810	Msa0600500	0.856909	2.338060e-62	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0600500	MtrunA17_Chr4g0021981	99.403	335	2	0	1	335	35	369	0.0	696
Msa0600500	MtrunA17_Chr4g0022021	85.373	335	49	0	1	335	35	369	0.0	613
Msa0600500	MtrunA17_Chr5g0432251	42.229	341	185	7	1	334	24	359	8.36e-82	252
Msa0600500	MtrunA17_Chr3g0121301	39.039	333	192	6	4	332	34	359	1.48e-81	252
Msa0600500	MtrunA17_Chr5g0432301	39.763	337	193	6	3	333	27	359	2.52e-78	243
Msa0600500	MtrunA17_Chr5g0432241	39.233	339	194	7	3	334	27	360	1.22e-72	229
Msa0600500	MtrunA17_Chr7g0276401	41.590	327	171	10	2	315	37	356	2.05e-72	229
Msa0600500	MtrunA17_Chr7g0276411	38.318	321	179	11	7	315	30	343	1.29e-70	224
Msa0600500	MtrunA17_Chr3g0128011	36.364	319	190	8	10	320	90	403	2.40e-67	217
Msa0600500	MtrunA17_Chr5g0448091	38.838	327	172	11	10	320	35	349	3.39e-67	215

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0600500	AT5G54160.1	38.318	321	185	7	8	321	34	348	9.68e-73	229
Msa0600500	AT1G77520.1	37.574	338	190	9	10	331	46	378	1.08e-64	209
Msa0600500	AT4G35160.1	34.393	346	206	6	4	333	40	380	1.17e-61	201
Msa0600500	AT1G51990.1	32.840	338	211	9	8	334	30	362	6.01e-57	189
Msa0600500	AT1G33030.1	36.760	321	186	11	10	320	23	336	9.88e-56	185
Msa0600500	AT1G63140.2	36.364	330	189	9	8	321	44	368	9.12e-55	184
Msa0600500	AT1G63140.4	36.474	329	188	9	9	321	65	388	1.61e-54	183
Msa0600500	AT1G51990.2	32.544	338	212	9	8	334	30	362	1.75e-54	182
Msa0600500	AT1G77530.1	37.353	340	192	10	8	331	44	378	2.94e-54	182
Msa0600500	AT4G35150.1	29.706	340	187	6	4	333	26	323	6.85e-53	177

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Find 109 sgRNAs with CRISPR-Local

Find 282 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATGTCTCCTCCAACAT+GGG	0.498630	4_2:+41986594	None:intergenic
AAACTTGATTAATAATAGGA+TGG	0.571512	4_2:+41987335	None:intergenic
AAAGGGGAATAAACAGTGTC+TGG	0.451909	4_2:-41987506	Msa0600500:five_prime_UTR
AAATACTTATTCAACGAGAC+AGG	0.536963	4_2:-41986190	Msa0600500:CDS
AAATTAAAGAAATTGGGTCA+TGG	0.525972	4_2:-41985752	Msa0600500:three_prime_UTR
AACAGGAAAGGTGATCATTG+TGG	0.615274	4_2:-41986326	Msa0600500:CDS
AAGAAAGAACTGAAGAGAAC+TGG	0.424963	4_2:-41986213	Msa0600500:CDS
AAGGCAATACCAGAGAAAAC+AGG	0.476074	4_2:-41986343	Msa0600500:CDS
AAGTCTTTGGTTGATGTTGG+CGG	0.462461	4_2:-41986727	Msa0600500:CDS
AAGTTAATAGCTTGAATGTG+AGG	0.610156	4_2:+41986664	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAATAGGAATCTTTGACCT+AGG	+	chr4_2:41985854-41985873	None:intergenic	30.0%
GTCAAAGATTCCTATTATGT+TGG	-	chr4_2:41985856-41985875	Msa0600500:three_prime_UTR	30.0%
ATTTGCGAATCCAACATAAT+AGG	+	chr4_2:41985869-41985888	None:intergenic	30.0%
TGCTTGGACAAATTGAAATA+TGG	-	chr4_2:41986009-41986028	Msa0600500:three_prime_UTR	30.0%
TTAAAAGTAGAGTGGAATCA+GGG	+	chr4_2:41986169-41986188	None:intergenic	30.0%
CATTACTCTTAAAAGTAGAG+TGG	+	chr4_2:41986177-41986196	None:intergenic	30.0%
TTTAAGAGTAATGAGAGTCA+TGG	-	chr4_2:41986184-41986203	Msa0600500:CDS	30.0%
ACAAGACACAAAAATGACAT+TGG	-	chr4_2:41986289-41986308	Msa0600500:CDS	30.0%
TTATTGCTGGATACAAAGAT+GGG	-	chr4_2:41986519-41986538	Msa0600500:intron	30.0%
CATCAACCAAAGACTTAATT+TGG	+	chr4_2:41986550-41986569	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	41985750	41987553	41985750	ID=Msa0600500;Name=Msa0600500
chr4_2	mRNA	41985750	41987553	41985750	ID=Msa0600500-mRNA-1;Parent=Msa0600500;Name=Msa0600500-mRNA-1;_AED=0.04;_eAED=0.04;_QI=288\|1\|1\|1\|1\|1\|2\|373\|335
chr4_2	exon	41985750	41986422	41985750	ID=Msa0600500-mRNA-1:exon:9146;Parent=Msa0600500-mRNA-1
chr4_2	exon	41986558	41987553	41986558	ID=Msa0600500-mRNA-1:exon:9145;Parent=Msa0600500-mRNA-1
chr4_2	five_prime_UTR	41987266	41987553	41987266	ID=Msa0600500-mRNA-1:five_prime_utr;Parent=Msa0600500-mRNA-1
chr4_2	CDS	41986558	41987265	41986558	ID=Msa0600500-mRNA-1:cds;Parent=Msa0600500-mRNA-1
chr4_2	CDS	41986123	41986422	41986123	ID=Msa0600500-mRNA-1:cds;Parent=Msa0600500-mRNA-1
chr4_2	three_prime_UTR	41985750	41986122	41985750	ID=Msa0600500-mRNA-1:three_prime_utr;Parent=Msa0600500-mRNA-1

Gene Sequence

>Msa0600500

ATGACACAATTCACAGACTCTTTTGCTCTAAAAGCTGTCGTGGAGCTACGCATAGCCGATATTATAGACCGTTATGGCAAACCACTATCCTTAACTCAAATTGTGCAGAATCTTGAAGATGCACCTTCCCCTGATTCCACTCTACTTTTAAGAGTAATGAGAGTCATGGTTCGTAGAAAGATTTTCAGTGCAGAAAAATCAGAAACCGGGGAAATTCTCTACGGCTTGACACGTGCATCAAAATGGATCCTACAAGACACAAAAATGACATTGGCACCAATGTTGTTGCTAGAGAATCACCCTTTTCATTTGAACCCTGCTAACTTTATTAGTGAAATTATAAAAGAAGGCACAACAAATGGCACTGCTTTCTTTAGGTGTCATGGTCATGAACAATTTGAAATGACGGGGTTGGACTCGAAATACAATGACTTGTTCAACCAAGGTATGGTTTGCACCGCTAGAATTGTGTCGAGGGCTGTTATTGCTGGATACAAAGATGGGTTTAACCAAATTAAGTCTTTGGTTGATGTTGGCGGGGGTATCGGAGGATCTCTCTCCGAGATTGTTAGAGCTTATCCTCACATTCAAGCTATTAACTTTGATTTGCCTCATGTGGTTTCAACGGCCCCTAATTTTGATGGTATCACCCATGTTGGAGGAGACATGTTTGTGTCAGTTCCTAGTGCTGATGCTATTTACATGAAGTGGATTCTTCATGACTGGAGCGACGACCATTGCATAAAGATATTGAAGAATTGTAGAAAGGCAATACCAGAGAAAACAGGAAAGGTGATCATTGTGGATCATGTATTGGACCCAGAAGGAAATGAACCATTTACCGACACTGGGATAGCATTTGACATGATGTTGCTTGCTCACAACGCTGGTGGTAAAGAAAGAACTGAAGAGAACTGGAAATACTTATTCAACGAGACAGGATTCCCTCGTTACAACATCATCAAAATCAATGCTCTGCCATGCATCATTGAGGCATTCCCAGTCTAA

Protein sequence

>Msa0600500

MTQFTDSFALKAVVELRIADIIDRYGKPLSLTQIVQNLEDAPSPDSTLLLRVMRVMVRRKIFSAEKSETGEILYGLTRASKWILQDTKMTLAPMLLLENHPFHLNPANFISEIIKEGTTNGTAFFRCHGHEQFEMTGLDSKYNDLFNQGMVCTARIVSRAVIAGYKDGFNQIKSLVDVGGGIGGSLSEIVRAYPHIQAINFDLPHVVSTAPNFDGITHVGGDMFVSVPSADAIYMKWILHDWSDDHCIKILKNCRKAIPEKTGKVIIVDHVLDPEGNEPFTDTGIAFDMMLLAHNAGGKERTEENWKYLFNETGFPRYNIIKINALPCIIEAFPV*