AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029227.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029227.01.T01 MTR_5g083010 98.305 177 2 1 1 176 1 177 1.47e-127 355
MsG0580029227.01.T01 MTR_3g071420 74.725 182 40 1 1 176 4 185 8.19e-89 258
MsG0580029227.01.T01 MTR_7g033800 50.000 150 68 1 8 150 8 157 9.25e-48 153
MsG0580029227.01.T01 MTR_4g105170 46.108 167 79 2 9 174 9 165 1.09e-45 149
MsG0580029227.01.T01 MTR_6g005070 64.151 106 38 0 8 113 6 111 1.59e-44 144
MsG0580029227.01.T01 MTR_3g077240 63.551 107 39 0 9 115 8 114 2.52e-44 145
MsG0580029227.01.T01 MTR_3g031660 54.545 121 54 1 8 128 4 123 9.55e-43 140
MsG0580029227.01.T01 MTR_6g005080 51.471 136 51 1 8 128 7 142 1.93e-42 139
MsG0580029227.01.T01 MTR_5g083230 63.366 101 37 0 8 108 3 103 3.15e-42 139
MsG0580029227.01.T01 MTR_3g071590 46.358 151 81 0 8 158 5 155 8.68e-42 137
MsG0580029227.01.T01 MTR_4g083680 60.396 101 40 0 8 108 12 112 1.20e-41 138
MsG0580029227.01.T01 MTR_2g068760 58.491 106 44 0 3 108 5 110 6.49e-41 135
MsG0580029227.01.T01 MTR_3g073690 60.952 105 41 0 8 112 6 110 4.75e-40 134
MsG0580029227.01.T01 MTR_5g083960 50.769 130 62 1 8 137 36 163 8.96e-40 134
MsG0580029227.01.T01 MTR_5g017950 60.952 105 41 0 8 112 5 109 1.02e-39 137
MsG0580029227.01.T01 MTR_3g452660 49.231 130 63 1 8 137 4 130 1.68e-38 133
MsG0580029227.01.T01 MTR_8g040900 53.788 132 56 1 8 134 4 135 2.22e-38 131
MsG0580029227.01.T01 MTR_2g093310 43.226 155 71 2 10 152 32 181 1.74e-36 125
MsG0580029227.01.T01 MTR_3g094690 56.863 102 44 0 12 113 48 149 4.88e-36 125
MsG0580029227.01.T01 MTR_7g096530 51.327 113 54 1 2 113 5 117 1.27e-35 124
MsG0580029227.01.T01 MTR_5g075020 49.640 139 58 2 4 136 30 162 4.09e-35 123
MsG0580029227.01.T01 MTR_8g036085 54.902 102 45 1 12 112 24 125 5.51e-35 122
MsG0580029227.01.T01 MTR_5g075020 49.640 139 58 2 4 136 34 166 5.80e-35 122
MsG0580029227.01.T01 MTR_8g036105 50.442 113 55 1 1 112 8 120 7.30e-35 122
MsG0580029227.01.T01 MTR_1g095850 55.446 101 45 0 12 112 73 173 1.09e-34 122
MsG0580029227.01.T01 MTR_7g074990 51.485 101 48 1 12 111 21 121 3.06e-34 120
MsG0580029227.01.T01 MTR_3g094690 55.882 102 44 1 12 113 48 148 1.72e-33 119
MsG0580029227.01.T01 MTR_1g070220 52.294 109 50 2 2 108 5 113 3.87e-33 117
MsG0580029227.01.T01 MTR_1g070205 38.095 168 91 4 12 167 13 179 4.75e-33 117
MsG0580029227.01.T01 MTR_4g060950 56.701 97 41 1 10 105 23 119 4.83e-33 116
MsG0580029227.01.T01 MTR_7g096610 50.893 112 53 2 8 117 5 116 5.29e-33 117
MsG0580029227.01.T01 MTR_6g027710 50.495 101 49 1 12 111 41 141 6.01e-33 117
MsG0580029227.01.T01 MTR_5g080470 40.000 125 74 1 9 132 5 129 1.15e-30 110
MsG0580029227.01.T01 MTR_7g075230 50.000 108 51 2 8 112 9 116 1.89e-30 110
MsG0580029227.01.T01 MTR_7g028905 43.689 103 58 0 10 112 4 106 1.52e-29 105
MsG0580029227.01.T01 MTR_8g079660 49.020 102 52 0 12 113 9 110 9.01e-29 107
MsG0580029227.01.T01 MTR_5g093010 42.574 101 58 0 12 112 6 106 5.69e-28 102
MsG0580029227.01.T01 MTR_8g079620 47.059 102 54 0 12 113 9 110 3.35e-27 102
MsG0580029227.01.T01 MTR_6g027700 44.898 98 53 1 12 108 8 105 4.02e-26 99.4
MsG0580029227.01.T01 MTR_4g099230 36.090 133 71 4 12 134 3 131 1.33e-23 91.3
MsG0580029227.01.T01 MTR_4g107450 32.075 159 104 2 8 166 7 161 1.83e-23 94.4
MsG0580029227.01.T01 MTR_6g011230 34.266 143 94 0 8 150 8 150 3.90e-23 91.3
MsG0580029227.01.T01 MTR_8g079580 43.689 103 57 1 12 113 9 111 1.02e-22 90.9
MsG0580029227.01.T01 MTR_2g100020 33.846 130 77 1 8 128 16 145 1.59e-22 91.3
MsG0580029227.01.T01 MTR_6g011200 37.615 109 67 1 7 114 5 113 1.05e-21 88.6
MsG0580029227.01.T01 MTR_8g017090 35.238 105 68 0 8 112 9 113 1.00e-20 87.0
MsG0580029227.01.T01 MTR_6g011250 36.697 109 68 1 7 114 5 113 4.95e-20 82.8
MsG0580029227.01.T01 MTR_5g085390 36.283 113 63 3 7 114 5 113 5.62e-18 78.6
MsG0580029227.01.T01 MTR_4g088035 31.111 90 62 0 8 97 3 92 2.32e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029227.01.T01 AT3G11090 63.399 153 53 2 1 151 1 152 1.61e-63 193
MsG0580029227.01.T01 AT5G63090 58.475 118 46 1 9 123 9 126 3.38e-44 145
MsG0580029227.01.T01 AT5G63090 58.475 118 46 1 9 123 9 126 3.38e-44 145
MsG0580029227.01.T01 AT5G63090 58.475 118 46 1 9 123 9 126 3.38e-44 145
MsG0580029227.01.T01 AT5G63090 58.475 118 46 1 9 123 9 126 3.38e-44 145
MsG0580029227.01.T01 AT5G66870 54.887 133 56 1 8 140 4 132 4.76e-44 148
MsG0580029227.01.T01 AT3G27650 57.759 116 49 0 8 123 36 151 1.35e-43 142
MsG0580029227.01.T01 AT2G30130 57.391 115 48 1 8 122 5 118 2.74e-41 137
MsG0580029227.01.T01 AT1G65620 56.800 125 51 1 8 129 6 130 1.59e-39 133
MsG0580029227.01.T01 AT1G65620 56.800 125 51 1 8 129 6 130 1.59e-39 133
MsG0580029227.01.T01 AT1G65620 56.800 125 51 1 8 129 6 130 1.59e-39 133
MsG0580029227.01.T01 AT1G65620 56.800 125 51 1 8 129 6 130 1.59e-39 133
MsG0580029227.01.T01 AT1G65620 56.800 125 51 1 8 129 6 130 1.59e-39 133
MsG0580029227.01.T01 AT2G23660 56.731 104 45 0 8 111 2 105 2.15e-39 136
MsG0580029227.01.T01 AT2G23660 56.731 104 45 0 8 111 2 105 2.15e-39 136
MsG0580029227.01.T01 AT2G23660 56.731 104 45 0 8 111 2 105 2.15e-39 136
MsG0580029227.01.T01 AT2G30340 54.701 117 52 1 12 127 53 169 4.67e-38 131
MsG0580029227.01.T01 AT2G30340 54.701 117 52 1 12 127 54 170 5.11e-38 131
MsG0580029227.01.T01 AT1G31320 60.000 90 36 0 8 97 10 99 1.26e-36 125
MsG0580029227.01.T01 AT1G07900 58.586 99 41 0 10 108 32 130 4.85e-36 124
MsG0580029227.01.T01 AT1G16530 51.786 112 52 2 2 111 4 115 1.13e-35 122
MsG0580029227.01.T01 AT2G28500 56.863 102 44 0 10 111 54 155 1.95e-35 124
MsG0580029227.01.T01 AT3G03760 52.427 103 48 1 10 111 50 152 3.48e-35 124
MsG0580029227.01.T01 AT2G40470 68.750 80 25 0 12 91 21 100 5.62e-35 122
MsG0580029227.01.T01 AT4G00210 50.442 113 55 1 1 112 1 113 6.62e-35 122
MsG0580029227.01.T01 AT2G40470 68.750 80 25 0 12 91 46 125 1.31e-34 121
MsG0580029227.01.T01 AT4G00220 56.000 100 43 1 14 112 20 119 1.98e-34 121
MsG0580029227.01.T01 AT2G42430 49.580 119 59 1 2 119 6 124 3.47e-34 121
MsG0580029227.01.T01 AT2G45420 53.000 100 46 1 14 112 40 139 7.78e-34 120
MsG0580029227.01.T01 AT3G58190 43.750 128 71 1 12 138 12 139 4.24e-33 117
MsG0580029227.01.T01 AT2G42440 46.552 116 61 1 8 122 4 119 4.84e-33 118
MsG0580029227.01.T01 AT3G26660 48.515 101 52 0 12 112 6 106 1.61e-32 113
MsG0580029227.01.T01 AT3G26620 48.515 101 52 0 12 112 6 106 2.05e-32 112
MsG0580029227.01.T01 AT5G06080 42.017 119 68 1 9 126 5 123 2.21e-31 112
MsG0580029227.01.T01 AT2G45410 51.000 100 48 1 14 112 19 118 6.58e-31 111
MsG0580029227.01.T01 AT2G31310 44.340 106 58 1 9 113 5 110 4.01e-30 108
MsG0580029227.01.T01 AT3G50510 40.602 133 77 2 7 137 8 140 1.67e-27 102
MsG0580029227.01.T01 AT3G50510 40.602 133 77 2 7 137 8 140 1.67e-27 102
MsG0580029227.01.T01 AT5G35900 38.686 137 80 1 8 140 2 138 2.54e-27 102
MsG0580029227.01.T01 AT3G13850 38.235 102 63 0 11 112 36 137 8.50e-24 94.7
MsG0580029227.01.T01 AT1G72980 38.739 111 68 0 8 118 10 120 4.91e-22 89.0
MsG0580029227.01.T01 AT3G47870 41.379 87 51 0 8 94 33 119 6.58e-20 85.1
MsG0580029227.01.T01 AT4G22700 35.036 137 81 4 8 138 2 136 2.24e-18 79.0
MsG0580029227.01.T01 AT1G06280 46.154 78 42 0 11 88 24 101 3.48e-18 78.6
MsG0580029227.01.T01 AT2G19820 35.165 91 57 2 12 101 13 102 3.09e-12 60.8
MsG0580029227.01.T01 AT5G15060 38.158 76 43 2 10 82 16 90 5.70e-12 60.8

Find 25 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGAAAGTGCACAAAGTATT+TGG 0.201773 5:-93974410 MsG0580029227.01.T01:CDS
GGAAGAAACTGTGAATTCTT+TGG 0.269255 5:-93974339 MsG0580029227.01.T01:CDS
TATGGCGCAAATATACAATT+TGG 0.301099 5:+93974461 None:intergenic
CACTCATCGGAGCGAAAGTA+TGG 0.344487 5:+93974443 None:intergenic
CGCGAATTTCTTGCACTCAT+CGG 0.347852 5:+93974430 None:intergenic
GTTTCTTCCCTTTGTTCCTC+TGG 0.351316 5:+93974353 None:intergenic
AGGCTTAGAGACCCCGTCTA+TGG 0.370197 5:-93974299 MsG0580029227.01.T01:CDS
ATTCGAACTCATCATATCAT+TGG 0.425834 5:+93974157 None:intergenic
TGCCATTGCAAAGGATCGTC+TGG 0.428280 5:-93974219 MsG0580029227.01.T01:CDS
TAACAGCATTAAAATATGTA+AGG 0.494256 5:+93974014 None:intergenic
GCACCTATGCATCCATAGAC+GGG 0.499649 5:+93974287 None:intergenic
CGAAGTTCCAGAGGAACAAA+GGG 0.517072 5:-93974360 MsG0580029227.01.T01:CDS
AAATTTGCAGGCTGCACAAG+AGG 0.545401 5:+93974502 None:intergenic
AGCACCTATGCATCCATAGA+CGG 0.565869 5:+93974286 None:intergenic
GCACAAGAGGAACTTGACCT+TGG 0.570179 5:+93974515 None:intergenic
TCGAAGTTCCAGAGGAACAA+AGG 0.580233 5:-93974361 MsG0580029227.01.T01:CDS
TGGCTTATGAAGCTGATGCA+AGG 0.584407 5:-93974319 MsG0580029227.01.T01:CDS
ACATGATCTTGCCATTGCAA+AGG 0.585696 5:-93974228 MsG0580029227.01.T01:CDS
CACTCTAGAGAAATCATGAA+GGG 0.616662 5:-93974545 None:intergenic
AGCCAGACGATCCTTTGCAA+TGG 0.625442 5:+93974217 None:intergenic
GACCCCGTCTATGGATGCAT+AGG 0.633413 5:-93974290 MsG0580029227.01.T01:CDS
TCATGAAGGGTTATGAACCA+AGG 0.655644 5:-93974532 MsG0580029227.01.T01:CDS
CACCTATGCATCCATAGACG+GGG 0.668606 5:+93974288 None:intergenic
GATTCTGATCGAAGTTCCAG+AGG 0.676488 5:-93974369 MsG0580029227.01.T01:CDS
TCGTCTGGCTCGTTGCGCTG+CGG 0.690033 5:-93974204 MsG0580029227.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATATCATTGGAAAAAGAAGT+AGG + Chr5:93974385-93974404 None:intergenic 25.0%
ATTCGAACTCATCATATCAT+TGG + Chr5:93974398-93974417 None:intergenic 30.0%
TATGGCGCAAATATACAATT+TGG + Chr5:93974094-93974113 None:intergenic 30.0%
!! TCTTCTTTTGAGAAATTTGC+AGG + Chr5:93974065-93974084 None:intergenic 30.0%
CAAGTGAAAAACTCAGGAAA+AGG + Chr5:93974433-93974452 None:intergenic 35.0%
GGAAGAAACTGTGAATTCTT+TGG - Chr5:93974213-93974232 MsG0580029227.01.T01:CDS 35.0%
ACATGATCTTGCCATTGCAA+AGG - Chr5:93974324-93974343 MsG0580029227.01.T01:CDS 40.0%
GTGAAAAACTCAGGAAAAGG+TGG + Chr5:93974430-93974449 None:intergenic 40.0%
TCACTGCAAGTGAAAAACTC+AGG + Chr5:93974439-93974458 None:intergenic 40.0%
! AGCACTTTTGCAAAGGAAGA+TGG - Chr5:93974291-93974310 MsG0580029227.01.T01:CDS 40.0%
! GCGAAAGTGCACAAAGTATT+TGG - Chr5:93974142-93974161 MsG0580029227.01.T01:CDS 40.0%
! GTGCTATAGCACTTTTGCAA+AGG - Chr5:93974284-93974303 MsG0580029227.01.T01:CDS 40.0%
AAATTTGCAGGCTGCACAAG+AGG + Chr5:93974053-93974072 None:intergenic 45.0%
AGCACCTATGCATCCATAGA+CGG + Chr5:93974269-93974288 None:intergenic 45.0%
CGAAGTTCCAGAGGAACAAA+GGG - Chr5:93974192-93974211 MsG0580029227.01.T01:CDS 45.0%
CGCGAATTTCTTGCACTCAT+CGG + Chr5:93974125-93974144 None:intergenic 45.0%
GATTCTGATCGAAGTTCCAG+AGG - Chr5:93974183-93974202 MsG0580029227.01.T01:CDS 45.0%
GTTTCTTCCCTTTGTTCCTC+TGG + Chr5:93974202-93974221 None:intergenic 45.0%
TCGAAGTTCCAGAGGAACAA+AGG - Chr5:93974191-93974210 MsG0580029227.01.T01:CDS 45.0%
TGGCTTATGAAGCTGATGCA+AGG - Chr5:93974233-93974252 MsG0580029227.01.T01:CDS 45.0%
AGCCAGACGATCCTTTGCAA+TGG + Chr5:93974338-93974357 None:intergenic 50.0%
CACCTATGCATCCATAGACG+GGG + Chr5:93974267-93974286 None:intergenic 50.0%
CACTCATCGGAGCGAAAGTA+TGG + Chr5:93974112-93974131 None:intergenic 50.0%
GCACAAGAGGAACTTGACCT+TGG + Chr5:93974040-93974059 None:intergenic 50.0%
GCACCTATGCATCCATAGAC+GGG + Chr5:93974268-93974287 None:intergenic 50.0%
TGCCATTGCAAAGGATCGTC+TGG - Chr5:93974333-93974352 MsG0580029227.01.T01:CDS 50.0%
AGGCTTAGAGACCCCGTCTA+TGG - Chr5:93974253-93974272 MsG0580029227.01.T01:CDS 55.0%
GACCCCGTCTATGGATGCAT+AGG - Chr5:93974262-93974281 MsG0580029227.01.T01:CDS 55.0%
! TCGTCTGGCTCGTTGCGCTG+CGG - Chr5:93974348-93974367 MsG0580029227.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 93974022 93974552 93974022 ID=MsG0580029227.01;Name=MsG0580029227.01
Chr5 mRNA 93974022 93974552 93974022 ID=MsG0580029227.01.T01;Parent=MsG0580029227.01;Name=MsG0580029227.01.T01;_AED=0.15;_eAED=0.15;_QI=0|-1|0|1|-1|1|1|0|176
Chr5 exon 93974022 93974552 93974022 ID=MsG0580029227.01.T01:exon:49758;Parent=MsG0580029227.01.T01
Chr5 CDS 93974022 93974552 93974022 ID=MsG0580029227.01.T01:cds;Parent=MsG0580029227.01.T01
Gene Sequence

>MsG0580029227.01.T01

ATGAAGGGTTATGAACCAAGGTCAAGTTCCTCTTGTGCAGCCTGCAAATTTCTCAAAAGAAGATGCATACCAAATTGTATATTTGCGCCATACTTTCGCTCCGATGAGTGCAAGAAATTCGCGAAAGTGCACAAAGTATTTGGAGCAAGCAATGTGAGCAAGATTCTGATCGAAGTTCCAGAGGAACAAAGGGAAGAAACTGTGAATTCTTTGGCTTATGAAGCTGATGCAAGGCTTAGAGACCCCGTCTATGGATGCATAGGTGCTATAGCACTTTTGCAAAGGAAGATGGTTGAACTTCAACATGATCTTGCCATTGCAAAGGATCGTCTGGCTCGTTGCGCTGCGGCTGCTGCTACTCCTACTTCTTTTTCCAATGATATGATGAGTTCGAATGTTAGTTTGCCACCTTTTCCTGAGTTTTTCACTTGCAGTGACTTCAGTGATAATTTAAGTCACAGTTCTTCGTCTCAATCGTTTAGTCGAAATGAAACTGTTGATGATTTCGTTCAAATTCCTTACATATTTTAA

Protein sequence

>MsG0580029227.01.T01

MKGYEPRSSSSCAACKFLKRRCIPNCIFAPYFRSDECKKFAKVHKVFGASNVSKILIEVPEEQREETVNSLAYEADARLRDPVYGCIGAIALLQRKMVELQHDLAIAKDRLARCAAAAATPTSFSNDMMSSNVSLPPFPEFFTCSDFSDNLSHSSSSQSFSRNETVDDFVQIPYIF*