AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000035

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000035.t1 MTR_3g034130 91.973 299 24 0 1 299 64 362 0.0 576
MS.gene000035.t1 MTR_3g034180 86.262 313 42 1 1 313 178 489 0.0 551
MS.gene000035.t1 MTR_3g034160 84.615 312 47 1 1 312 64 374 0.0 515
MS.gene000035.t1 MTR_3g103020 62.420 314 103 6 3 312 75 377 2.19e-125 363
MS.gene000035.t1 MTR_4g117720 59.938 322 105 10 1 312 70 377 1.57e-119 348
MS.gene000035.t1 MTR_6g060650 58.696 322 112 6 1 312 62 372 1.02e-114 336
MS.gene000035.t1 MTR_4g109280 60.317 315 106 8 1 312 60 358 2.25e-106 315
MS.gene000035.t1 MTR_4g122850 54.574 317 127 9 6 316 62 367 4.00e-106 315
MS.gene000035.t1 MTR_4g109270 55.975 318 116 8 1 312 64 363 3.15e-104 309
MS.gene000035.t1 MTR_4g094365 52.978 319 127 8 1 313 63 364 3.38e-98 294
MS.gene000035.t1 MTR_4g023080 53.503 314 120 8 1 312 64 353 3.25e-96 288
MS.gene000035.t1 MTR_4g117850 54.194 310 118 9 7 310 85 376 6.83e-96 288
MS.gene000035.t1 MTR_4g117770 54.603 315 115 7 9 313 71 367 2.09e-93 282
MS.gene000035.t1 MTR_4g117810 54.603 315 115 7 9 313 71 367 2.09e-93 282
MS.gene000035.t1 MTR_4g023090 49.249 333 132 9 1 313 71 386 9.54e-93 281
MS.gene000035.t1 MTR_4g118380 52.769 307 113 6 1 286 62 357 2.39e-92 279
MS.gene000035.t1 MTR_4g116330 51.780 309 128 7 10 312 86 379 4.15e-90 274
MS.gene000035.t1 MTR_4g021785 51.623 308 124 7 7 312 70 354 6.50e-90 272
MS.gene000035.t1 MTR_4g023070 48.795 332 138 10 1 316 58 373 2.67e-87 266
MS.gene000035.t1 MTR_4g117780 50.464 323 129 8 1 313 66 367 4.96e-87 265
MS.gene000035.t1 MTR_5g097210 48.867 309 142 6 3 310 67 360 4.50e-86 263
MS.gene000035.t1 MTR_4g023380 47.761 335 115 9 7 313 77 379 8.91e-85 260
MS.gene000035.t1 MTR_5g070080 48.048 333 135 8 4 312 67 385 8.21e-84 258
MS.gene000035.t1 MTR_5g070130 48.773 326 141 10 3 315 67 379 8.99e-84 258
MS.gene000035.t1 MTR_4g117870 48.726 314 113 7 1 313 1 267 1.55e-83 253
MS.gene000035.t1 MTR_4g117750 48.137 322 138 7 3 315 60 361 4.76e-83 259
MS.gene000035.t1 MTR_4g117750 56.637 113 47 1 205 315 373 485 1.14e-32 126
MS.gene000035.t1 MTR_4g023120 48.328 329 138 11 1 313 58 370 3.96e-82 253
MS.gene000035.t1 MTR_4g023190 50.629 318 125 11 1 311 65 357 1.39e-81 251
MS.gene000035.t1 MTR_1g055135 47.134 314 146 8 7 312 73 374 2.11e-81 251
MS.gene000035.t1 MTR_4g023130 51.389 288 116 7 27 312 47 312 4.26e-81 248
MS.gene000035.t1 MTR_8g433040 49.527 317 127 9 4 313 74 364 5.08e-80 247
MS.gene000035.t1 MTR_8g032380 49.527 317 127 9 4 313 74 364 5.08e-80 247
MS.gene000035.t1 MTR_4g023200 49.057 318 131 11 1 311 65 358 7.72e-79 244
MS.gene000035.t1 MTR_4g023150 47.950 317 136 10 1 311 65 358 1.12e-76 239
MS.gene000035.t1 MTR_1g055195 47.213 305 130 7 20 316 200 481 2.12e-76 242
MS.gene000035.t1 MTR_4g023440 45.687 313 116 7 1 313 74 332 1.21e-74 233
MS.gene000035.t1 MTR_8g032390 47.152 316 119 11 2 308 94 370 1.92e-72 228
MS.gene000035.t1 MTR_5g070088 43.582 335 154 8 3 313 63 386 4.14e-72 228
MS.gene000035.t1 MTR_4g116320 56.250 224 83 6 91 310 43 255 1.80e-71 222
MS.gene000035.t1 MTR_4g118400 46.667 300 114 8 16 307 33 294 1.10e-70 221
MS.gene000035.t1 MTR_8g013010 57.870 216 74 6 1 214 109 309 2.38e-68 216
MS.gene000035.t1 MTR_8g040935 53.302 212 87 4 107 312 2 207 1.11e-64 203
MS.gene000035.t1 MTR_8g433030 44.231 312 101 10 2 308 94 337 3.10e-63 203
MS.gene000035.t1 MTR_4g118370 46.667 255 104 7 58 310 33 257 4.60e-63 200
MS.gene000035.t1 MTR_7g079400 41.558 308 157 8 14 312 43 336 8.40e-62 200
MS.gene000035.t1 MTR_4g116330 43.366 309 115 8 10 312 86 340 2.54e-60 196
MS.gene000035.t1 MTR_7g079370 40.184 326 163 9 7 315 48 358 1.80e-58 192
MS.gene000035.t1 MTR_1g055165 39.936 313 124 8 3 312 66 317 8.89e-57 186
MS.gene000035.t1 MTR_4g121580 42.810 306 125 13 7 303 75 339 2.49e-52 177
MS.gene000035.t1 MTR_6g463430 48.095 210 93 5 109 313 4 202 2.55e-52 171
MS.gene000035.t1 MTR_1g055175 48.241 199 93 5 118 312 8 200 1.47e-51 169
MS.gene000035.t1 MTR_8g102520 38.924 316 141 10 7 312 48 321 2.69e-50 169
MS.gene000035.t1 MTR_1g055250 42.424 231 118 8 74 300 34 253 1.22e-49 168
MS.gene000035.t1 MTR_4g117530 41.473 258 91 8 63 312 47 252 7.66e-48 161
MS.gene000035.t1 MTR_4g117430 51.534 163 56 5 57 217 14 155 1.38e-43 147
MS.gene000035.t1 MTR_4g114700 38.519 270 103 8 42 304 5 218 1.01e-40 142
MS.gene000035.t1 MTR_2g048580 32.686 309 162 11 10 312 73 341 1.86e-39 142
MS.gene000035.t1 MTR_4g118360 57.480 127 48 3 190 312 1 125 5.70e-39 134
MS.gene000035.t1 MTR_4g116340 47.727 176 76 5 10 180 83 247 1.26e-38 137
MS.gene000035.t1 MTR_7g079420 34.520 281 129 10 45 312 905 1143 6.83e-35 134
MS.gene000035.t1 MTR_8g098830 42.529 174 86 5 114 282 30 194 8.92e-35 126
MS.gene000035.t1 MTR_5g023000 62.105 95 36 0 218 312 4 98 4.45e-32 115
MS.gene000035.t1 MTR_7g117720 52.778 144 52 7 7 145 52 184 2.39e-30 116
MS.gene000035.t1 MTR_7g117720 38.000 150 79 6 164 308 132 272 3.91e-14 71.6
MS.gene000035.t1 MTR_4g122860 38.424 203 79 7 123 315 156 322 9.92e-30 115
MS.gene000035.t1 MTR_6g034720 41.053 190 89 7 40 223 31 203 1.35e-27 107
MS.gene000035.t1 MTR_7g079450 41.333 150 63 5 174 312 1 136 1.36e-24 97.1
MS.gene000035.t1 MTR_1g068875 28.814 236 140 10 21 243 71 291 5.91e-22 95.5
MS.gene000035.t1 MTR_4g122840 42.647 136 40 4 181 313 164 264 4.78e-21 90.9
MS.gene000035.t1 MTR_5g069120 44.186 129 63 2 6 127 51 177 1.15e-18 82.4
MS.gene000035.t1 MTR_4g023430 54.878 82 34 1 235 313 141 222 1.38e-17 80.5
MS.gene000035.t1 MTR_8g013005 67.241 58 19 0 255 312 1 58 1.63e-17 75.9
MS.gene000035.t1 MTR_4g133170 29.630 216 123 11 36 236 82 283 2.35e-17 82.4
MS.gene000035.t1 MTR_0667s0010 24.742 291 178 10 6 268 57 334 4.64e-17 81.6
MS.gene000035.t1 MTR_2g024110 25.000 244 153 9 7 236 60 287 1.12e-16 80.1
MS.gene000035.t1 MTR_2g026010 30.137 219 128 10 35 236 81 291 1.20e-16 80.1
MS.gene000035.t1 MTR_4g070550 26.744 258 144 10 13 236 63 309 2.16e-16 79.3
MS.gene000035.t1 MTR_2g028160 27.660 235 140 9 57 274 130 351 2.30e-16 79.7
MS.gene000035.t1 MTR_2g021155 29.508 183 113 5 59 237 711 881 3.93e-16 79.7
MS.gene000035.t1 MTR_2g021155 29.648 199 122 7 44 237 131 316 7.74e-12 66.6
MS.gene000035.t1 MTR_2g028130 26.400 250 146 10 37 261 100 336 8.29e-16 77.8
MS.gene000035.t1 MTR_2g031640 26.068 234 144 8 18 236 70 289 4.45e-15 75.5
MS.gene000035.t1 MTR_2g025960 27.354 223 123 9 39 236 1 209 5.47e-15 74.7
MS.gene000035.t1 MTR_2g078870 31.844 179 97 7 59 237 78 231 5.63e-15 74.7
MS.gene000035.t1 MTR_2g426070 25.954 262 152 12 39 277 101 343 1.35e-14 74.3
MS.gene000035.t1 MTR_1g055200 48.837 86 39 2 42 127 5 85 2.31e-14 68.6
MS.gene000035.t1 MTR_4g055060 27.935 247 150 9 8 243 93 322 4.78e-14 72.4
MS.gene000035.t1 MTR_2g426090 25.112 223 132 9 37 236 94 304 9.75e-14 71.6
MS.gene000035.t1 MTR_1g068835 26.230 244 150 11 16 243 63 292 9.83e-14 71.6
MS.gene000035.t1 MTR_2g028140 26.027 219 125 8 44 236 108 315 1.06e-13 71.6
MS.gene000035.t1 MTR_1g068860 23.629 237 154 8 21 243 71 294 1.80e-13 70.9
MS.gene000035.t1 MTR_3g083010 23.792 269 163 9 37 276 102 357 4.18e-13 69.7
MS.gene000035.t1 MTR_1g054725 30.769 208 112 11 43 237 117 305 8.69e-13 68.6
MS.gene000035.t1 MTR_2g078900 30.387 181 99 8 59 237 153 308 1.63e-12 67.8
MS.gene000035.t1 MTR_2g025420 23.948 309 177 13 6 278 67 353 2.21e-12 67.8
MS.gene000035.t1 MTR_1g094210 28.520 277 146 14 22 284 140 378 2.23e-12 67.8
MS.gene000035.t1 MTR_0044s0300 28.780 205 107 11 44 237 138 314 2.43e-12 67.4
MS.gene000035.t1 MTR_4g117550 27.675 271 145 12 57 311 139 374 2.48e-12 67.4
MS.gene000035.t1 MTR_7g070830 26.446 242 131 12 57 264 147 375 2.76e-12 67.4
MS.gene000035.t1 MTR_8g464770 25.080 311 192 13 17 307 88 377 2.90e-12 67.0
MS.gene000035.t1 MTR_4g028510 27.336 289 156 11 53 308 133 400 3.05e-12 67.4
MS.gene000035.t1 MTR_1g097560 25.623 281 176 11 7 281 99 352 3.82e-12 66.6
MS.gene000035.t1 MTR_6g053260 27.404 208 130 7 34 237 429 619 3.96e-12 67.4
MS.gene000035.t1 MTR_4g023100 61.404 57 20 1 257 313 65 119 4.00e-12 62.8
MS.gene000035.t1 MTR_2g021150 26.357 258 166 9 15 264 87 328 4.56e-12 66.6
MS.gene000035.t1 MTR_3g036290 28.986 207 114 9 41 243 117 294 7.27e-12 65.9
MS.gene000035.t1 MTR_2g024100 26.016 246 139 10 20 236 73 304 8.56e-12 65.9
MS.gene000035.t1 MTR_3g007330 28.700 223 127 9 32 243 127 328 1.32e-11 65.1
MS.gene000035.t1 MTR_3g009310 27.835 194 109 7 57 243 158 327 1.41e-11 65.1
MS.gene000035.t1 MTR_4g109290 60.377 53 21 0 260 312 135 187 1.41e-11 62.8
MS.gene000035.t1 MTR_3g013740 27.941 204 121 7 44 237 150 337 1.89e-11 64.7
MS.gene000035.t1 MTR_2g021190 26.786 224 143 8 20 237 95 303 2.05e-11 64.7
MS.gene000035.t1 MTR_3g007410 27.751 209 127 8 44 243 146 339 2.10e-11 64.7
MS.gene000035.t1 MTR_0129s0070 25.806 279 170 11 57 313 136 399 2.21e-11 64.7
MS.gene000035.t1 MTR_2g031680 26.027 219 135 8 33 236 80 286 2.75e-11 64.3
MS.gene000035.t1 MTR_2g021252 25.105 239 147 7 3 237 105 315 2.78e-11 64.3
MS.gene000035.t1 MTR_1g094210 28.413 271 142 14 22 278 140 372 2.84e-11 64.3
MS.gene000035.t1 MTR_3g007360 27.230 213 127 8 44 245 146 341 2.89e-11 64.3
MS.gene000035.t1 MTR_2g461450 26.872 227 140 8 17 236 82 289 3.10e-11 63.9
MS.gene000035.t1 MTR_3g006670 29.557 203 116 9 44 237 147 331 3.73e-11 63.9
MS.gene000035.t1 MTR_5g086590 25.214 234 140 9 57 281 14 221 4.45e-11 62.4
MS.gene000035.t1 MTR_3g010920 25.097 259 146 9 58 304 158 380 5.86e-11 63.2
MS.gene000035.t1 MTR_2g031720 24.561 228 147 7 24 236 76 293 6.56e-11 63.2
MS.gene000035.t1 MTR_2g064960 65.854 41 14 0 272 312 18 58 6.98e-11 57.4
MS.gene000035.t1 MTR_3g030320 29.064 203 109 11 44 237 46 222 7.17e-11 62.4
MS.gene000035.t1 MTR_4g030830 25.688 218 117 10 44 236 113 310 8.76e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000035.t1 AT4G22390 25.670 261 169 10 40 282 90 343 6.80e-12 66.2
MS.gene000035.t1 AT4G22390 25.670 261 169 10 40 282 90 343 7.61e-12 65.9

Find 68 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAGAATTTCTCAGTCTA+TGG 0.212417 3.1:-24674744 MS.gene000035:CDS
ACAAAATGTGGTCATATTAT+TGG 0.222453 3.1:-24674595 MS.gene000035:CDS
GATTGTGATTTGTGGATATT+TGG 0.226311 3.1:-24674763 MS.gene000035:CDS
TACTCACTTCCCTTATTATA+TGG 0.240722 3.1:-24674947 MS.gene000035:CDS
TTATGTTGTCTGTATGGTTT+TGG 0.252091 3.1:-24675123 MS.gene000035:CDS
GGGATGAAACAATTGAAATA+TGG 0.254108 3.1:-24674714 MS.gene000035:CDS
TCATTCGCGTCCATATAATA+AGG 0.269202 3.1:+24674937 None:intergenic
ATTCGGGAAGCGTAAAATTA+AGG 0.294949 3.1:+24675286 None:intergenic
ACCAATGAATAGTCTCATTA+AGG 0.315052 3.1:+24674893 None:intergenic
ATTAAGGAGCGTCCCTACTT+TGG 0.366607 3.1:+24674909 None:intergenic
TTCAAGATTAGAATAAGATT+CGG 0.367334 3.1:+24675269 None:intergenic
TATTGTTGGCTTTGATTTAG+TGG 0.370118 3.1:-24674839 MS.gene000035:CDS
TTAAGGAGCGTCCCTACTTT+GGG 0.370759 3.1:+24674910 None:intergenic
AAATATTTATGTTGTCTGTA+TGG 0.373648 3.1:-24675129 MS.gene000035:CDS
TCAAGATTAGAATAAGATTC+GGG 0.401464 3.1:+24675270 None:intergenic
TGGGTCTATACTTGTATCAT+AGG 0.413780 3.1:+24675059 None:intergenic
ACTGCTGTCCTATCTATTGA+TGG 0.424599 3.1:-24674652 MS.gene000035:CDS
AACCTATAGATTGTGATTTG+TGG 0.425796 3.1:-24674771 MS.gene000035:CDS
GGATGAAACAATTGAAATAT+GGG 0.428889 3.1:-24674713 MS.gene000035:CDS
ATTGGAACAAATGGTGGTAC+TGG 0.446274 3.1:-24674577 MS.gene000035:CDS
TCCTTAATGAGACTATTCAT+TGG 0.450606 3.1:-24674894 MS.gene000035:CDS
AATCACAATCTATAGGTTCA+TGG 0.456461 3.1:+24674776 None:intergenic
GGTTCATGGTCAAAACCATC+TGG 0.456773 3.1:+24674790 None:intergenic
TCTAAATTGGAAGGAGGAAA+AGG 0.457817 3.1:+24675156 None:intergenic
AGTACAAAGTGCATTCATCT+TGG 0.459425 3.1:-24674681 MS.gene000035:CDS
ATCCACAAATCACAATCTAT+AGG 0.460799 3.1:+24674769 None:intergenic
TGCAAATGCCATCAATAGAT+AGG 0.461230 3.1:+24674644 None:intergenic
TTTCTCAGTCTATGGGCTAT+GGG 0.463750 3.1:-24674736 MS.gene000035:CDS
ATTCTTTACACATCTACCTA+TGG 0.464376 3.1:-24675209 MS.gene000035:CDS
ATAGATCGAATTTCAAGAGT+TGG 0.464410 3.1:+24675357 None:intergenic
ATTTCTCAGTCTATGGGCTA+TGG 0.471238 3.1:-24674737 MS.gene000035:CDS
TTATCAATGGATGTTATTGT+TGG 0.479970 3.1:-24674853 MS.gene000035:CDS
CATTCGCGTCCATATAATAA+GGG 0.483397 3.1:+24674938 None:intergenic
TAACATCCATTGATAAATCA+TGG 0.489522 3.1:+24674860 None:intergenic
TGGAGAATTTCTCAGTCTAT+GGG 0.490268 3.1:-24674743 MS.gene000035:CDS
CTCTGTATACATAGCCACTA+GGG 0.491428 3.1:+24674486 None:intergenic
TGAGTATACTTCACCAATCC+AGG 0.492432 3.1:+24674964 None:intergenic
CCATTCAAGAGATGATTACT+TGG 0.492530 3.1:-24675094 MS.gene000035:CDS
TCTCGTTGAGAGCTAATACA+TGG 0.498785 3.1:-24675002 MS.gene000035:CDS
GGTCATATTATTGGAACAAA+TGG 0.505789 3.1:-24674586 MS.gene000035:CDS
TGGTCAAAACCATCTGGAAA+AGG 0.508561 3.1:+24674796 None:intergenic
CCAAGTAATCATCTCTTGAA+TGG 0.518868 3.1:+24675094 None:intergenic
TGGAACGAAATTGAGTGTCC+TGG 0.524624 3.1:-24674982 MS.gene000035:CDS
TTTGTATCTAAATTGGAAGG+AGG 0.529658 3.1:+24675150 None:intergenic
TCAATTTGCTCATTGTCACC+AGG 0.530329 3.1:+24674448 None:intergenic
CGAAATTGAGTGTCCTGGAT+TGG 0.536602 3.1:-24674977 MS.gene000035:CDS
GAAATTAAAAGTTCATGTAG+AGG 0.538733 3.1:-24675249 MS.gene000035:CDS
GCGAATGATGATCCCAAAGT+AGG 0.546958 3.1:-24674922 MS.gene000035:CDS
TGTTTGCAACGTCCAGTGGA+TGG 0.550128 3.1:+24675183 None:intergenic
TATTTGTTTGCAACGTCCAG+TGG 0.551784 3.1:+24675179 None:intergenic
TACCTATGGAATCCATCCAC+TGG 0.559625 3.1:-24675195 MS.gene000035:CDS
TTCAAGAGATGATTACTTGG+TGG 0.560339 3.1:-24675091 MS.gene000035:CDS
TTCTCAGTCTATGGGCTATG+GGG 0.565567 3.1:-24674735 MS.gene000035:CDS
TTGGCTTTGATTTAGTGGAA+AGG 0.565737 3.1:-24674834 MS.gene000035:CDS
CAATTTGCTCATTGTCACCA+GGG 0.568496 3.1:+24674449 None:intergenic
CGAATGATGATCCCAAAGTA+GGG 0.573102 3.1:-24674921 MS.gene000035:CDS
CCAATATGCTCTACAAAATG+TGG 0.574609 3.1:-24674607 MS.gene000035:CDS
GAGCAAATTGAAGAAGAATG+AGG 0.587599 3.1:-24674436 None:intergenic
TCTCAGTCTATGGGCTATGG+GGG 0.592269 3.1:-24674734 MS.gene000035:CDS
CATATTATTGGAACAAATGG+TGG 0.593852 3.1:-24674583 MS.gene000035:CDS
AACAAATGGTGGTACTGGAT+TGG 0.613049 3.1:-24674572 MS.gene000035:CDS
ACTCTGTATACATAGCCACT+AGG 0.618200 3.1:+24674485 None:intergenic
TGAACCATGTGCACCCCTAG+TGG 0.636728 3.1:-24674500 MS.gene000035:CDS
ATAGCCACTAGGGGTGCACA+TGG 0.643218 3.1:+24674496 None:intergenic
GTCCAGTGGATGGATTCCAT+AGG 0.675230 3.1:+24675193 None:intergenic
TCTGTATACATAGCCACTAG+GGG 0.698983 3.1:+24674487 None:intergenic
TTGGTGAAGTACGATGGCAA+TGG 0.721153 3.1:-24674553 MS.gene000035:CDS
ACTGGATTGGTGAAGTACGA+TGG 0.727911 3.1:-24674559 MS.gene000035:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGAAATATTATCTTTTATT+GGG + chr3.1:24674767-24674786 None:intergenic 10.0%
!!! TAAATATTTTGTATCTAAAT+TGG + chr3.1:24674664-24674683 None:intergenic 10.0%
!!! GATGAAATATTATCTTTTAT+TGG + chr3.1:24674768-24674787 None:intergenic 15.0%
!! AAATATTTATGTTGTCTGTA+TGG - chr3.1:24674675-24674694 MS.gene000035:CDS 20.0%
!! TTCAAGATTAGAATAAGATT+CGG + chr3.1:24674538-24674557 None:intergenic 20.0%
!!! TATTTTGTATCTAAATTGGA+AGG + chr3.1:24674660-24674679 None:intergenic 20.0%
! ACAAAATGTGGTCATATTAT+TGG - chr3.1:24675209-24675228 MS.gene000035:CDS 25.0%
! GAAATTAAAAGTTCATGTAG+AGG - chr3.1:24674555-24674574 MS.gene000035:CDS 25.0%
! GGATGAAACAATTGAAATAT+GGG - chr3.1:24675091-24675110 MS.gene000035:CDS 25.0%
! TAACATCCATTGATAAATCA+TGG + chr3.1:24674947-24674966 None:intergenic 25.0%
! TCAAGATTAGAATAAGATTC+GGG + chr3.1:24674537-24674556 None:intergenic 25.0%
! TTATCAATGGATGTTATTGT+TGG - chr3.1:24674951-24674970 MS.gene000035:CDS 25.0%
AACCTATAGATTGTGATTTG+TGG - chr3.1:24675033-24675052 MS.gene000035:CDS 30.0%
AATCACAATCTATAGGTTCA+TGG + chr3.1:24675031-24675050 None:intergenic 30.0%
ACCAATGAATAGTCTCATTA+AGG + chr3.1:24674914-24674933 None:intergenic 30.0%
ATAGATCGAATTTCAAGAGT+TGG + chr3.1:24674450-24674469 None:intergenic 30.0%
ATCCACAAATCACAATCTAT+AGG + chr3.1:24675038-24675057 None:intergenic 30.0%
ATTCTTTACACATCTACCTA+TGG - chr3.1:24674595-24674614 MS.gene000035:CDS 30.0%
CATATTATTGGAACAAATGG+TGG - chr3.1:24675221-24675240 MS.gene000035:CDS 30.0%
GGGATGAAACAATTGAAATA+TGG - chr3.1:24675090-24675109 MS.gene000035:CDS 30.0%
GGTCATATTATTGGAACAAA+TGG - chr3.1:24675218-24675237 MS.gene000035:CDS 30.0%
TACTCACTTCCCTTATTATA+TGG - chr3.1:24674857-24674876 MS.gene000035:CDS 30.0%
TCCTTAATGAGACTATTCAT+TGG - chr3.1:24674910-24674929 MS.gene000035:CDS 30.0%
TTTGTATCTAAATTGGAAGG+AGG + chr3.1:24674657-24674676 None:intergenic 30.0%
! ACATTTTGTAGAGCATATTG+GGG + chr3.1:24675198-24675217 None:intergenic 30.0%
! CACATTTTGTAGAGCATATT+GGG + chr3.1:24675199-24675218 None:intergenic 30.0%
! GATTGTGATTTGTGGATATT+TGG - chr3.1:24675041-24675060 MS.gene000035:CDS 30.0%
! TATGTTGTCTGTATGGTTTT+GGG - chr3.1:24674682-24674701 MS.gene000035:CDS 30.0%
! TTATGTTGTCTGTATGGTTT+TGG - chr3.1:24674681-24674700 MS.gene000035:CDS 30.0%
! TTTTCGCCATGATTTATCAA+TGG - chr3.1:24674938-24674957 MS.gene000035:CDS 30.0%
!! TATTGTTGGCTTTGATTTAG+TGG - chr3.1:24674965-24674984 MS.gene000035:CDS 30.0%
AGTACAAAGTGCATTCATCT+TGG - chr3.1:24675123-24675142 MS.gene000035:CDS 35.0%
CATTCGCGTCCATATAATAA+GGG + chr3.1:24674869-24674888 None:intergenic 35.0%
CCAAGTAATCATCTCTTGAA+TGG + chr3.1:24674713-24674732 None:intergenic 35.0%
CCAATATGCTCTACAAAATG+TGG - chr3.1:24675197-24675216 MS.gene000035:CDS 35.0%
CCATTCAAGAGATGATTACT+TGG - chr3.1:24674710-24674729 MS.gene000035:CDS 35.0%
TCATTCGCGTCCATATAATA+AGG + chr3.1:24674870-24674889 None:intergenic 35.0%
TCTAAATTGGAAGGAGGAAA+AGG + chr3.1:24674651-24674670 None:intergenic 35.0%
TGCAAATGCCATCAATAGAT+AGG + chr3.1:24675163-24675182 None:intergenic 35.0%
TGGAGAATTTCTCAGTCTAT+GGG - chr3.1:24675061-24675080 MS.gene000035:CDS 35.0%
TGGGTCTATACTTGTATCAT+AGG + chr3.1:24674748-24674767 None:intergenic 35.0%
TTCAAGAGATGATTACTTGG+TGG - chr3.1:24674713-24674732 MS.gene000035:CDS 35.0%
TTGGAGAATTTCTCAGTCTA+TGG - chr3.1:24675060-24675079 MS.gene000035:CDS 35.0%
! ATTCGGGAAGCGTAAAATTA+AGG + chr3.1:24674521-24674540 None:intergenic 35.0%
! CATTTTGTAGAGCATATTGG+GGG + chr3.1:24675197-24675216 None:intergenic 35.0%
! CCACATTTTGTAGAGCATAT+TGG + chr3.1:24675200-24675219 None:intergenic 35.0%
!! TTGGCTTTGATTTAGTGGAA+AGG - chr3.1:24674970-24674989 MS.gene000035:CDS 35.0%
ACTCTGTATACATAGCCACT+AGG + chr3.1:24675322-24675341 None:intergenic 40.0%
ACTGCTGTCCTATCTATTGA+TGG - chr3.1:24675152-24675171 MS.gene000035:CDS 40.0%
ATTGGAACAAATGGTGGTAC+TGG - chr3.1:24675227-24675246 MS.gene000035:CDS 40.0%
CGAATGATGATCCCAAAGTA+GGG - chr3.1:24674883-24674902 MS.gene000035:CDS 40.0%
CTCTGTATACATAGCCACTA+GGG + chr3.1:24675321-24675340 None:intergenic 40.0%
TATTTGTTTGCAACGTCCAG+TGG + chr3.1:24674628-24674647 None:intergenic 40.0%
TCTCGTTGAGAGCTAATACA+TGG - chr3.1:24674802-24674821 MS.gene000035:CDS 40.0%
TCTGTATACATAGCCACTAG+GGG + chr3.1:24675320-24675339 None:intergenic 40.0%
TGAGTATACTTCACCAATCC+AGG + chr3.1:24674843-24674862 None:intergenic 40.0%
TGGTCAAAACCATCTGGAAA+AGG + chr3.1:24675011-24675030 None:intergenic 40.0%
! AACAAATGGTGGTACTGGAT+TGG - chr3.1:24675232-24675251 MS.gene000035:CDS 40.0%
! ATTTCTCAGTCTATGGGCTA+TGG - chr3.1:24675067-24675086 MS.gene000035:CDS 40.0%
! CAATTTGCTCATTGTCACCA+GGG + chr3.1:24675358-24675377 None:intergenic 40.0%
! TCAATTTGCTCATTGTCACC+AGG + chr3.1:24675359-24675378 None:intergenic 40.0%
! TTAGAGATGCCTTTTCCAGA+TGG - chr3.1:24674999-24675018 MS.gene000035:CDS 40.0%
! TTTCTCAGTCTATGGGCTAT+GGG - chr3.1:24675068-24675087 MS.gene000035:CDS 40.0%
CGAAATTGAGTGTCCTGGAT+TGG - chr3.1:24674827-24674846 MS.gene000035:CDS 45.0%
GCGAATGATGATCCCAAAGT+AGG - chr3.1:24674882-24674901 MS.gene000035:CDS 45.0%
GGTTCATGGTCAAAACCATC+TGG + chr3.1:24675017-24675036 None:intergenic 45.0%
TACCTATGGAATCCATCCAC+TGG - chr3.1:24674609-24674628 MS.gene000035:CDS 45.0%
TGGAACGAAATTGAGTGTCC+TGG - chr3.1:24674822-24674841 MS.gene000035:CDS 45.0%
TTGGTGAAGTACGATGGCAA+TGG - chr3.1:24675251-24675270 MS.gene000035:CDS 45.0%
! TTCTCAGTCTATGGGCTATG+GGG - chr3.1:24675069-24675088 MS.gene000035:CDS 45.0%
!! ACTGGATTGGTGAAGTACGA+TGG - chr3.1:24675245-24675264 MS.gene000035:CDS 45.0%
!! ATTAAGGAGCGTCCCTACTT+TGG + chr3.1:24674898-24674917 None:intergenic 45.0%
!! TTAAGGAGCGTCCCTACTTT+GGG + chr3.1:24674897-24674916 None:intergenic 45.0%
TGTTTGCAACGTCCAGTGGA+TGG + chr3.1:24674624-24674643 None:intergenic 50.0%
! GTCCAGTGGATGGATTCCAT+AGG + chr3.1:24674614-24674633 None:intergenic 50.0%
! TCTCAGTCTATGGGCTATGG+GGG - chr3.1:24675070-24675089 MS.gene000035:CDS 50.0%
!! GAGTCTCTGCTTTTACTCCC+TGG - chr3.1:24675338-24675357 MS.gene000035:CDS 50.0%
TGAACCATGTGCACCCCTAG+TGG - chr3.1:24675304-24675323 MS.gene000035:CDS 55.0%
! ATAGCCACTAGGGGTGCACA+TGG + chr3.1:24675311-24675330 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 24674438 24675388 24674438 ID=MS.gene000035
chr3.1 mRNA 24674438 24675388 24674438 ID=MS.gene000035.t1;Parent=MS.gene000035
chr3.1 exon 24674438 24675388 24674438 ID=MS.gene000035.t1.exon1;Parent=MS.gene000035.t1
chr3.1 CDS 24674438 24675388 24674438 ID=cds.MS.gene000035.t1;Parent=MS.gene000035.t1
Gene Sequence

>MS.gene000035

ATGTCAACTCCAACTCTTGAAATTCGATCTATAGATTTTGAAGCATCGCTTAACGATGATTCTGCTTCTATTTCACTGAACCTTAATTTTACGCTTCCCGAATCTTATTCTAATCTTGAAATTAAAAGTTCATGTAGAGGATTTATAGTTTTGACTTATTCTTTACACATCTACCTATGGAATCCATCCACTGGACGTTGCAAACAAATACCTTTTCCTCCTTCCAATTTAGATACAAAATATTTATGTTGTCTGTATGGTTTTGGGTACGACCATTCAAGAGATGATTACTTGGTGGTATCAGTGTCCTATGATACAAGTATAGACCCAATAAAAGATAATATTTCATCACATTTGAAGTTTTTCTCGTTGAGAGCTAATACATGGAACGAAATTGAGTGTCCTGGATTGGTGAAGTATACTCACTTCCCTTATTATATGGACGCGAATGATGATCCCAAAGTAGGGACGCTCCTTAATGAGACTATTCATTGGTTCTCTTTTCGCCATGATTTATCAATGGATGTTATTGTTGGCTTTGATTTAGTGGAAAGGAAACTCTTAGAGATGCCTTTTCCAGATGGTTTTGACCATGAACCTATAGATTGTGATTTGTGGATATTTGGAGAATTTCTCAGTCTATGGGCTATGGGGGATGAAACAATTGAAATATGGGTTATGAAAGAGTACAAAGTGCATTCATCTTGGACTACGACTGCTGTCCTATCTATTGATGGCATTTGCATTCAGTATGATCCCCCAATATGCTCTACAAAATGTGGTCATATTATTGGAACAAATGGTGGTACTGGATTGGTGAAGTACGATGGCAATGGTCAGTTGCTAGAGAATCGCTCGTACTATAATGAACCATGTGCACCCCTAGTGGCTATGTATACAGAGTCTCTGCTTTTACTCCCTGGTGACAATGAGCAAATTGAAGAAGAATGA
Protein sequence

>MS.gene000035.t1

MSTPTLEIRSIDFEASLNDDSASISLNLNFTLPESYSNLEIKSSCRGFIVLTYSLHIYLWNPSTGRCKQIPFPPSNLDTKYLCCLYGFGYDHSRDDYLVVSVSYDTSIDPIKDNISSHLKFFSLRANTWNEIECPGLVKYTHFPYYMDANDDPKVGTLLNETIHWFSFRHDLSMDVIVGFDLVERKLLEMPFPDGFDHEPIDCDLWIFGEFLSLWAMGDETIEIWVMKEYKVHSSWTTTAVLSIDGICIQYDPPICSTKCGHIIGTNGGTGLVKYDGNGQLLENRSYYNEPCAPLVAMYTESLLLLPGDNEQIEEE