AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87569

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87569.t1 MTR_4g023120 90.574 244 23 0 1 244 127 370 8.52e-165 460
MS.gene87569.t1 MTR_4g023070 89.712 243 25 0 1 243 127 369 3.95e-162 453
MS.gene87569.t1 MTR_4g023090 60.924 238 85 3 10 244 154 386 9.50e-98 290
MS.gene87569.t1 MTR_4g023130 61.885 244 69 4 1 243 92 312 1.61e-95 282
MS.gene87569.t1 MTR_4g021785 59.756 246 74 4 1 244 133 355 1.50e-94 281
MS.gene87569.t1 MTR_4g023080 61.066 244 71 4 1 243 133 353 2.88e-94 281
MS.gene87569.t1 MTR_4g023380 56.391 266 61 4 2 244 146 379 2.09e-93 279
MS.gene87569.t1 MTR_4g023150 53.878 245 90 5 1 242 134 358 4.39e-77 237
MS.gene87569.t1 MTR_4g023200 53.469 245 91 5 1 242 134 358 1.43e-76 236
MS.gene87569.t1 MTR_3g103020 55.785 242 77 8 13 244 157 378 1.76e-76 236
MS.gene87569.t1 MTR_4g023190 54.286 245 88 6 1 242 134 357 5.33e-76 234
MS.gene87569.t1 MTR_4g116330 51.626 246 96 6 1 241 150 377 3.51e-71 223
MS.gene87569.t1 MTR_4g117720 51.984 252 89 10 2 241 144 375 4.35e-71 222
MS.gene87569.t1 MTR_6g060650 51.271 236 92 7 12 243 156 372 3.79e-70 219
MS.gene87569.t1 MTR_4g023440 50.000 244 67 3 1 244 144 332 4.11e-70 218
MS.gene87569.t1 MTR_4g094365 50.988 253 95 8 1 244 132 364 4.54e-69 216
MS.gene87569.t1 MTR_4g117850 51.867 241 87 8 12 241 154 376 4.37e-68 214
MS.gene87569.t1 MTR_8g032390 54.274 234 84 8 11 239 155 370 1.48e-65 208
MS.gene87569.t1 MTR_8g433030 54.545 231 82 8 14 239 125 337 2.68e-65 206
MS.gene87569.t1 MTR_4g109280 52.885 208 76 6 43 244 168 359 2.69e-65 207
MS.gene87569.t1 MTR_3g034180 48.819 254 103 8 2 244 252 489 8.84e-64 206
MS.gene87569.t1 MTR_4g109270 50.209 239 90 7 12 244 149 364 1.49e-63 202
MS.gene87569.t1 MTR_4g116320 51.082 231 88 6 18 241 43 255 5.66e-62 195
MS.gene87569.t1 MTR_6g463430 54.146 205 73 4 44 244 15 202 1.06e-61 192
MS.gene87569.t1 MTR_3g034130 50.000 236 88 8 2 225 138 355 1.51e-61 197
MS.gene87569.t1 MTR_3g034160 49.407 253 97 9 2 241 138 372 6.68e-61 196
MS.gene87569.t1 MTR_8g433040 50.000 238 88 7 11 244 154 364 1.32e-60 195
MS.gene87569.t1 MTR_8g032380 50.000 238 88 7 11 244 154 364 1.32e-60 195
MS.gene87569.t1 MTR_4g117780 48.333 240 99 6 11 244 147 367 3.10e-60 194
MS.gene87569.t1 MTR_5g097210 50.209 239 92 9 11 241 141 360 8.24e-60 193
MS.gene87569.t1 MTR_8g040935 50.952 210 82 5 43 244 12 208 2.88e-59 186
MS.gene87569.t1 MTR_4g117770 49.372 239 96 6 12 244 148 367 2.90e-59 191
MS.gene87569.t1 MTR_4g117810 49.372 239 96 6 12 244 148 367 2.90e-59 191
MS.gene87569.t1 MTR_4g122850 50.000 244 92 12 11 244 141 364 1.11e-58 190
MS.gene87569.t1 MTR_4g118380 49.545 220 83 6 10 219 156 357 1.78e-57 186
MS.gene87569.t1 MTR_4g117870 45.749 247 80 9 1 244 72 267 7.13e-56 180
MS.gene87569.t1 MTR_5g070130 44.262 244 109 6 5 237 142 369 2.99e-54 179
MS.gene87569.t1 MTR_5g070080 47.881 236 92 8 13 239 167 380 1.10e-53 177
MS.gene87569.t1 MTR_1g055135 44.130 247 109 8 10 244 146 375 2.19e-51 171
MS.gene87569.t1 MTR_5g070088 45.022 231 110 5 13 239 163 380 2.41e-51 171
MS.gene87569.t1 MTR_1g055195 43.265 245 100 7 9 241 258 475 6.10e-51 173
MS.gene87569.t1 MTR_4g118400 48.500 200 71 6 42 237 122 293 2.05e-50 166
MS.gene87569.t1 MTR_4g116330 43.089 246 78 6 1 241 150 338 4.39e-49 164
MS.gene87569.t1 MTR_4g118370 42.500 240 96 9 9 241 53 257 5.42e-49 162
MS.gene87569.t1 MTR_4g121580 45.607 239 79 10 11 243 155 348 9.27e-47 160
MS.gene87569.t1 MTR_4g117750 44.082 245 107 8 9 240 128 355 1.22e-44 156
MS.gene87569.t1 MTR_4g117750 47.222 144 55 3 108 242 350 481 6.19e-28 111
MS.gene87569.t1 MTR_1g055250 40.650 246 114 9 10 244 46 270 2.71e-44 152
MS.gene87569.t1 MTR_1g055175 43.578 218 92 8 37 242 1 199 1.24e-43 146
MS.gene87569.t1 MTR_4g117530 40.426 235 86 6 11 244 72 253 6.09e-42 143
MS.gene87569.t1 MTR_7g079370 41.702 235 112 8 12 237 131 349 1.06e-39 140
MS.gene87569.t1 MTR_7g079400 38.372 258 126 10 1 244 99 337 6.87e-39 138
MS.gene87569.t1 MTR_1g055165 45.714 175 71 5 76 244 162 318 8.66e-38 134
MS.gene87569.t1 MTR_8g102520 37.008 254 131 10 1 244 88 322 4.42e-35 127
MS.gene87569.t1 MTR_4g114700 38.362 232 72 6 12 235 50 218 9.42e-34 122
MS.gene87569.t1 MTR_4g117430 51.613 124 46 5 12 133 41 152 3.36e-30 110
MS.gene87569.t1 MTR_8g013010 50.794 126 47 5 12 133 195 309 2.55e-29 112
MS.gene87569.t1 MTR_4g023430 47.727 132 37 4 121 244 115 222 6.61e-28 106
MS.gene87569.t1 MTR_4g118360 47.482 139 52 5 108 241 1 123 9.66e-28 103
MS.gene87569.t1 MTR_4g122860 38.350 206 74 9 50 244 157 320 1.58e-27 108
MS.gene87569.t1 MTR_5g023000 52.727 110 38 2 137 244 2 99 1.73e-27 101
MS.gene87569.t1 MTR_2g048580 34.091 220 107 8 30 237 142 335 1.22e-24 100
MS.gene87569.t1 MTR_8g098830 36.364 198 99 9 45 235 35 212 2.70e-24 97.1
MS.gene87569.t1 MTR_7g079420 33.054 239 106 10 12 242 951 1143 1.64e-22 96.7
MS.gene87569.t1 MTR_7g079450 38.462 156 74 6 92 244 1 137 7.22e-22 88.2
MS.gene87569.t1 MTR_4g116340 54.545 88 36 3 12 98 163 247 9.07e-22 90.9
MS.gene87569.t1 MTR_4g023100 74.545 55 14 0 190 244 65 119 1.47e-20 84.3
MS.gene87569.t1 MTR_6g034720 47.475 99 50 2 12 109 72 169 1.56e-20 87.0
MS.gene87569.t1 MTR_7g117720 45.902 122 47 4 122 237 162 270 5.64e-20 86.7
MS.gene87569.t1 MTR_7g117720 58.182 55 21 1 11 65 132 184 1.24e-11 63.5
MS.gene87569.t1 MTR_4g133170 32.418 182 101 7 12 183 142 311 2.47e-18 83.6
MS.gene87569.t1 MTR_2g021050 26.984 252 143 9 1 240 119 341 5.17e-17 79.7
MS.gene87569.t1 MTR_1g068875 30.857 175 109 7 12 179 139 308 2.67e-16 77.4
MS.gene87569.t1 MTR_8g013005 62.069 58 20 1 188 243 1 58 5.77e-16 70.5
MS.gene87569.t1 MTR_1g094210 24.537 216 138 5 3 216 185 377 9.99e-16 76.3
MS.gene87569.t1 MTR_1g094210 25.316 237 151 6 3 236 185 398 1.29e-15 75.9
MS.gene87569.t1 MTR_2g426020 32.203 177 110 6 7 180 155 324 1.37e-15 75.5
MS.gene87569.t1 MTR_2g028130 27.957 186 117 7 4 179 155 333 2.28e-15 75.1
MS.gene87569.t1 MTR_0667s0010 27.895 190 118 7 2 179 142 324 2.39e-15 75.1
MS.gene87569.t1 MTR_1g068860 31.667 180 102 9 12 179 141 311 7.37e-15 73.6
MS.gene87569.t1 MTR_2g028160 29.947 187 110 8 7 180 158 336 3.40e-14 71.6
MS.gene87569.t1 MTR_1g068835 28.037 214 135 8 5 213 133 332 4.52e-14 71.2
MS.gene87569.t1 MTR_2g024110 27.778 180 111 6 12 179 139 311 6.50e-14 70.9
MS.gene87569.t1 MTR_2g025960 29.714 175 107 7 12 179 68 233 1.39e-13 69.3
MS.gene87569.t1 MTR_2g025420 28.342 187 107 8 10 179 164 340 2.12e-13 69.3
MS.gene87569.t1 MTR_6g090180 28.994 169 103 4 11 163 206 373 2.32e-13 69.3
MS.gene87569.t1 MTR_2g425980 27.160 243 137 10 10 235 156 375 5.57e-13 68.2
MS.gene87569.t1 MTR_2g028110 24.638 207 136 5 10 213 145 334 1.06e-12 67.0
MS.gene87569.t1 MTR_8g101580 25.214 234 150 8 9 236 180 394 2.02e-12 66.2
MS.gene87569.t1 MTR_7g055953 28.054 221 115 10 13 211 90 288 2.19e-12 65.9
MS.gene87569.t1 MTR_3g009310 27.234 235 145 8 5 236 179 390 3.03e-12 65.9
MS.gene87569.t1 MTR_2g021155 28.365 208 127 8 7 209 167 357 3.17e-12 66.2
MS.gene87569.t1 MTR_4g055060 31.737 167 97 7 13 173 176 331 5.13e-12 65.1
MS.gene87569.t1 MTR_4g061340 27.363 201 116 8 11 194 166 353 6.14e-12 65.1
MS.gene87569.t1 MTR_2g047832 25.688 218 125 8 1 197 159 360 6.64e-12 65.1
MS.gene87569.t1 MTR_2g046860 25.389 193 125 8 9 197 51 228 8.35e-12 64.3
MS.gene87569.t1 MTR_4g028510 25.506 247 141 7 12 239 178 400 9.67e-12 64.7
MS.gene87569.t1 MTR_0129s0050 27.820 266 135 10 12 241 164 408 1.10e-11 64.3
MS.gene87569.t1 MTR_7g062100 27.642 246 138 9 13 238 173 398 1.36e-11 63.9
MS.gene87569.t1 MTR_7g034310 27.049 244 129 12 13 238 17 229 1.58e-11 62.4
MS.gene87569.t1 MTR_4g117550 25.781 256 122 10 8 242 166 374 1.59e-11 63.5
MS.gene87569.t1 MTR_2g426060 28.333 180 113 7 10 179 157 330 2.35e-11 63.2
MS.gene87569.t1 MTR_1g055145 50.000 76 33 3 76 150 131 202 3.81e-11 62.0
MS.gene87569.t1 MTR_4g109290 62.264 53 18 1 194 244 136 188 3.82e-11 60.8
MS.gene87569.t1 MTR_1g097330 26.108 203 126 7 13 209 152 336 4.63e-11 62.4
MS.gene87569.t1 MTR_2g022000 26.587 252 142 11 7 233 174 407 4.77e-11 62.4
MS.gene87569.t1 MTR_7g017130 31.214 173 101 9 10 168 170 338 4.79e-11 62.4
MS.gene87569.t1 MTR_7g017170 31.214 173 101 9 10 168 170 338 5.42e-11 62.4
MS.gene87569.t1 MTR_6g065510 25.980 204 114 9 13 197 104 289 5.43e-11 62.0
MS.gene87569.t1 MTR_1g097490 24.444 225 147 6 13 236 188 390 7.61e-11 61.6
MS.gene87569.t1 MTR_5g064700 22.481 258 161 10 4 241 130 368 9.69e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 54 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGAATGGATTAACTTTAT+TGG 0.141488 4.1:+75188712 None:intergenic
ACAGAAAATGGTGATATTAT+TGG 0.164193 4.1:-75188230 MS.gene87569:CDS
CATTCTGGTTTGTGGGTATT+TGG 0.166612 4.1:-75188440 MS.gene87569:CDS
GAAGCGTATATTAATCTATA+TGG 0.223056 4.1:-75188782 MS.gene87569:CDS
GTAATGATACAATTGAAATA+TGG 0.247553 4.1:-75188385 MS.gene87569:CDS
CCAATGTATTCTACAGAAAA+TGG 0.266416 4.1:-75188242 MS.gene87569:CDS
TTGGAGAATTTCTCAGTCTA+TGG 0.269491 4.1:-75188421 MS.gene87569:CDS
CATTGTTGGCTTTGATTTAA+CGG 0.305680 4.1:-75188519 MS.gene87569:CDS
CACAGAGCATTAGTACATTC+TGG 0.320173 4.1:-75188455 MS.gene87569:CDS
GGTGATATTATTGGAAAATA+TGG 0.325508 4.1:-75188221 MS.gene87569:CDS
CTTCCTCAGCAAGTCATTCC+TGG 0.346410 4.1:-75188314 MS.gene87569:CDS
GTGAAAGTAGTCCGCAAATC+TGG 0.373146 4.1:+75188265 None:intergenic
TTTGCGTCGTGATATAGTTT+TGG 0.373784 4.1:-75188546 MS.gene87569:CDS
GAGGGTAATCACTTACCTTA+TGG 0.385311 4.1:-75188626 MS.gene87569:CDS
TATATTAATCTATATGGGTT+TGG 0.387173 4.1:-75188776 MS.gene87569:CDS
AGTCTATGGGTTAAGGATAC+TGG 0.398340 4.1:-75188407 MS.gene87569:CDS
TGGTGACAATGAGCATGTTT+AGG 0.402786 4.1:-75188087 None:intergenic
TGGTGTTCCTCAATCACATT+TGG 0.406622 4.1:-75188687 MS.gene87569:CDS
ATTTCTCAGTCTATGGGTTA+AGG 0.409114 4.1:-75188414 MS.gene87569:CDS
GCTCTGTGATCAACATCATT+TGG 0.411538 4.1:+75188470 None:intergenic
AAAGTTAATCCATTCTTAGT+TGG 0.417793 4.1:-75188707 MS.gene87569:CDS
TAATGATACAATTGAAATAT+GGG 0.423786 4.1:-75188384 MS.gene87569:CDS
ATTAGTACATTCTGGTTTGT+GGG 0.428082 4.1:-75188447 MS.gene87569:CDS
CATTAGTACATTCTGGTTTG+TGG 0.428815 4.1:-75188448 MS.gene87569:CDS
AAGCGTATATTAATCTATAT+GGG 0.430207 4.1:-75188781 MS.gene87569:CDS
GAGTCGCTACTTTCACTCTC+TGG 0.439571 4.1:-75188107 MS.gene87569:CDS
TCTCGTTTAGAGACAATACT+TGG 0.472530 4.1:-75188658 MS.gene87569:CDS
TGATCAACATCATTTGGAAC+AGG 0.489346 4.1:+75188476 None:intergenic
AATACAAAGTGCATTCGTCT+TGG 0.494278 4.1:-75188352 MS.gene87569:CDS
ATGATTATAGAGAAGGAGAA+GGG 0.495201 4.1:-75188601 MS.gene87569:CDS
TGGAGAATTTCTCAGTCTAT+GGG 0.497173 4.1:-75188420 MS.gene87569:CDS
TGAGGAACACCAACTAAGAA+TGG 0.498401 4.1:+75188698 None:intergenic
TCTCTATAATCATCGCCATA+AGG 0.509139 4.1:+75188611 None:intergenic
ATATAGATTAATATACGCTT+CGG 0.513373 4.1:+75188784 None:intergenic
AAAATATGGTGGTACTAGAT+TGG 0.540379 4.1:-75188207 MS.gene87569:CDS
TTATAGAGAAGGAGAAGGGG+TGG 0.546396 4.1:-75188597 MS.gene87569:CDS
GATGATTATAGAGAAGGAGA+AGG 0.547437 4.1:-75188602 MS.gene87569:CDS
TCTGTACTATTCCAGATTTG+CGG 0.550677 4.1:-75188276 MS.gene87569:CDS
CTCTGAATACACGACAACTT+CGG 0.554316 4.1:+75188127 None:intergenic
TATGGCGATGATTATAGAGA+AGG 0.582441 4.1:-75188608 MS.gene87569:CDS
GAATACACGACAACTTCGGA+TGG 0.585294 4.1:+75188131 None:intergenic
TTGGCTTTGATTTAACGGAA+AGG 0.585773 4.1:-75188514 MS.gene87569:CDS
TTCGGATGGACTGTTGCAGA+AGG 0.589220 4.1:+75188145 None:intergenic
GTCTAGAGATGATTATGTGG+TGG 0.603968 4.1:-75188744 MS.gene87569:CDS
CAAATCTGGAATAGTACAGA+GGG 0.610389 4.1:+75188279 None:intergenic
GCAAATCTGGAATAGTACAG+AGG 0.612314 4.1:+75188278 None:intergenic
TGATTATAGAGAAGGAGAAG+GGG 0.613001 4.1:-75188600 MS.gene87569:CDS
CAATACTTGGAAAGAAATCG+AGG 0.619297 4.1:-75188645 MS.gene87569:CDS
TGACCAGGAATGACTTGCTG+AGG 0.633416 4.1:+75188311 None:intergenic
TTGGTGAAGTATAATGACAA+AGG 0.639076 4.1:-75188188 MS.gene87569:CDS
GATATTATTGGAAAATATGG+TGG 0.639099 4.1:-75188218 MS.gene87569:CDS
AGAGGGTTAAAGTAGTGACC+AGG 0.655673 4.1:+75188296 None:intergenic
AATACTTGGAAAGAAATCGA+GGG 0.666838 4.1:-75188644 MS.gene87569:CDS
TCAGTCTAGAGATGATTATG+TGG 0.684477 4.1:-75188747 MS.gene87569:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGATACAATTGAAATAT+GGG - chr4.1:75188504-75188523 MS.gene87569:CDS 15.0%
!! AAGCGTATATTAATCTATAT+GGG - chr4.1:75188107-75188126 MS.gene87569:CDS 20.0%
!! ATATAGATTAATATACGCTT+CGG + chr4.1:75188107-75188126 None:intergenic 20.0%
!! GTAATGATACAATTGAAATA+TGG - chr4.1:75188503-75188522 MS.gene87569:CDS 20.0%
!! TATATTAATCTATATGGGTT+TGG - chr4.1:75188112-75188131 MS.gene87569:CDS 20.0%
!!! TAAGAATGGATTAACTTTAT+TGG + chr4.1:75188179-75188198 None:intergenic 20.0%
! AAAGTTAATCCATTCTTAGT+TGG - chr4.1:75188181-75188200 MS.gene87569:CDS 25.0%
! ACAGAAAATGGTGATATTAT+TGG - chr4.1:75188658-75188677 MS.gene87569:CDS 25.0%
! GAAGCGTATATTAATCTATA+TGG - chr4.1:75188106-75188125 MS.gene87569:CDS 25.0%
! GATATTATTGGAAAATATGG+TGG - chr4.1:75188670-75188689 MS.gene87569:CDS 25.0%
! GGTGATATTATTGGAAAATA+TGG - chr4.1:75188667-75188686 MS.gene87569:CDS 25.0%
!! CATTTTCTGTAGAATACATT+GGG + chr4.1:75188648-75188667 None:intergenic 25.0%
AAAAACTCCAAATGTGATTG+AGG + chr4.1:75188211-75188230 None:intergenic 30.0%
AAAATATGGTGGTACTAGAT+TGG - chr4.1:75188681-75188700 MS.gene87569:CDS 30.0%
AATACTTGGAAAGAAATCGA+GGG - chr4.1:75188244-75188263 MS.gene87569:CDS 30.0%
ATGATTATAGAGAAGGAGAA+GGG - chr4.1:75188287-75188306 MS.gene87569:CDS 30.0%
CCAATGTATTCTACAGAAAA+TGG - chr4.1:75188646-75188665 MS.gene87569:CDS 30.0%
TTGGTGAAGTATAATGACAA+AGG - chr4.1:75188700-75188719 MS.gene87569:CDS 30.0%
! AATGACAAAGGACAGTTTTT+AGG - chr4.1:75188712-75188731 MS.gene87569:CDS 30.0%
! ATGACAAAGGACAGTTTTTA+GGG - chr4.1:75188713-75188732 MS.gene87569:CDS 30.0%
! CCATTTTCTGTAGAATACAT+TGG + chr4.1:75188649-75188668 None:intergenic 30.0%
! TTTTTAATGGGGCTATTCAT+TGG - chr4.1:75188314-75188333 MS.gene87569:CDS 30.0%
!! ATTAGTACATTCTGGTTTGT+GGG - chr4.1:75188441-75188460 MS.gene87569:CDS 30.0%
!! CATTGTTGGCTTTGATTTAA+CGG - chr4.1:75188369-75188388 MS.gene87569:CDS 30.0%
!!! ATAGTTTTGGATGTCATTGT+TGG - chr4.1:75188355-75188374 MS.gene87569:CDS 30.0%
AATACAAAGTGCATTCGTCT+TGG - chr4.1:75188536-75188555 MS.gene87569:CDS 35.0%
CAATACTTGGAAAGAAATCG+AGG - chr4.1:75188243-75188262 MS.gene87569:CDS 35.0%
GATGATTATAGAGAAGGAGA+AGG - chr4.1:75188286-75188305 MS.gene87569:CDS 35.0%
TATGGCGATGATTATAGAGA+AGG - chr4.1:75188280-75188299 MS.gene87569:CDS 35.0%
TCAGTCTAGAGATGATTATG+TGG - chr4.1:75188141-75188160 MS.gene87569:CDS 35.0%
TCTCGTTTAGAGACAATACT+TGG - chr4.1:75188230-75188249 MS.gene87569:CDS 35.0%
TCTCTATAATCATCGCCATA+AGG + chr4.1:75188280-75188299 None:intergenic 35.0%
TCTGTACTATTCCAGATTTG+CGG - chr4.1:75188612-75188631 MS.gene87569:CDS 35.0%
TGATTATAGAGAAGGAGAAG+GGG - chr4.1:75188288-75188307 MS.gene87569:CDS 35.0%
TGGAGAATTTCTCAGTCTAT+GGG - chr4.1:75188468-75188487 MS.gene87569:CDS 35.0%
TTGGAGAATTTCTCAGTCTA+TGG - chr4.1:75188467-75188486 MS.gene87569:CDS 35.0%
! ATTTCTCAGTCTATGGGTTA+AGG - chr4.1:75188474-75188493 MS.gene87569:CDS 35.0%
! CAAATCTGGAATAGTACAGA+GGG + chr4.1:75188612-75188631 None:intergenic 35.0%
! TGATCAACATCATTTGGAAC+AGG + chr4.1:75188415-75188434 None:intergenic 35.0%
! TTTGCGTCGTGATATAGTTT+TGG - chr4.1:75188342-75188361 MS.gene87569:CDS 35.0%
!! CATTAGTACATTCTGGTTTG+TGG - chr4.1:75188440-75188459 MS.gene87569:CDS 35.0%
!! TTGGCTTTGATTTAACGGAA+AGG - chr4.1:75188374-75188393 MS.gene87569:CDS 35.0%
CTCTGAATACACGACAACTT+CGG + chr4.1:75188764-75188783 None:intergenic 40.0%
GAGGGTAATCACTTACCTTA+TGG - chr4.1:75188262-75188281 MS.gene87569:CDS 40.0%
GCTCTGTGATCAACATCATT+TGG + chr4.1:75188421-75188440 None:intergenic 40.0%
GTCTAGAGATGATTATGTGG+TGG - chr4.1:75188144-75188163 MS.gene87569:CDS 40.0%
TGAGGAACACCAACTAAGAA+TGG + chr4.1:75188193-75188212 None:intergenic 40.0%
TGGTGTTCCTCAATCACATT+TGG - chr4.1:75188201-75188220 MS.gene87569:CDS 40.0%
! AGAAGGGGTGGTTTTTAATG+GGG - chr4.1:75188303-75188322 MS.gene87569:CDS 40.0%
! AGTCTATGGGTTAAGGATAC+TGG - chr4.1:75188481-75188500 MS.gene87569:CDS 40.0%
! CACAGAGCATTAGTACATTC+TGG - chr4.1:75188433-75188452 MS.gene87569:CDS 40.0%
! GAGAAGGGGTGGTTTTTAAT+GGG - chr4.1:75188302-75188321 MS.gene87569:CDS 40.0%
! GCAAATCTGGAATAGTACAG+AGG + chr4.1:75188613-75188632 None:intergenic 40.0%
!! CATTCTGGTTTGTGGGTATT+TGG - chr4.1:75188448-75188467 MS.gene87569:CDS 40.0%
AGAGGGTTAAAGTAGTGACC+AGG + chr4.1:75188595-75188614 None:intergenic 45.0%
GAATACACGACAACTTCGGA+TGG + chr4.1:75188760-75188779 None:intergenic 45.0%
GTGAAAGTAGTCCGCAAATC+TGG + chr4.1:75188626-75188645 None:intergenic 45.0%
TTATAGAGAAGGAGAAGGGG+TGG - chr4.1:75188291-75188310 MS.gene87569:CDS 45.0%
! GGAGAAGGGGTGGTTTTTAA+TGG - chr4.1:75188301-75188320 MS.gene87569:CDS 45.0%
CTTCCTCAGCAAGTCATTCC+TGG - chr4.1:75188574-75188593 MS.gene87569:CDS 50.0%
! GAGTCGCTACTTTCACTCTC+TGG - chr4.1:75188781-75188800 MS.gene87569:CDS 50.0%
!! TGACCAGGAATGACTTGCTG+AGG + chr4.1:75188580-75188599 None:intergenic 50.0%
!! TTCGGATGGACTGTTGCAGA+AGG + chr4.1:75188746-75188765 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 75188088 75188822 75188088 ID=MS.gene87569
chr4.1 mRNA 75188088 75188822 75188088 ID=MS.gene87569.t1;Parent=MS.gene87569
chr4.1 exon 75188088 75188822 75188088 ID=MS.gene87569.t1.exon1;Parent=MS.gene87569.t1
chr4.1 CDS 75188088 75188822 75188088 ID=cds.MS.gene87569.t1;Parent=MS.gene87569.t1
Gene Sequence

>MS.gene87569

ATGTCTGCTTTTAATTCCGAAGCGTATATTAATCTATATGGGTTTGGTTATGATCAGTCTAGAGATGATTATGTGGTGGTTTTATTGTCCAATAAAGTTAATCCATTCTTAGTTGGTGTTCCTCAATCACATTTGGAGTTTTTCTCGTTTAGAGACAATACTTGGAAAGAAATCGAGGGTAATCACTTACCTTATGGCGATGATTATAGAGAAGGAGAAGGGGTGGTTTTTAATGGGGCTATTCATTGGTTGTCTTTGCGTCGTGATATAGTTTTGGATGTCATTGTTGGCTTTGATTTAACGGAAAGGAAATTGTTTGAGATGCCTGTTCCAAATGATGTTGATCACAGAGCATTAGTACATTCTGGTTTGTGGGTATTTGGAGAATTTCTCAGTCTATGGGTTAAGGATACTGGTAATGATACAATTGAAATATGGGTTATGAAAGAATACAAAGTGCATTCGTCTTGGACTAAGACTCTTGTTCTTCCTCAGCAAGTCATTCCTGGTCACTACTTTAACCCTCTGTACTATTCCAGATTTGCGGACTACTTTCACCCAATGTATTCTACAGAAAATGGTGATATTATTGGAAAATATGGTGGTACTAGATTGGTGAAGTATAATGACAAAGGACAGTTTTTAGGGCATCGCTCCTTCTGCAACAGTCCATCCGAAGTTGTCGTGTATTCAGAGTCGCTACTTTCACTCTCTGGTGACAATGAGCATGTTTAG
Protein sequence

>MS.gene87569.t1

MSAFNSEAYINLYGFGYDQSRDDYVVVLLSNKVNPFLVGVPQSHLEFFSFRDNTWKEIEGNHLPYGDDYREGEGVVFNGAIHWLSLRRDIVLDVIVGFDLTERKLFEMPVPNDVDHRALVHSGLWVFGEFLSLWVKDTGNDTIEIWVMKEYKVHSSWTKTLVLPQQVIPGHYFNPLYYSRFADYFHPMYSTENGDIIGKYGGTRLVKYNDKGQFLGHRSFCNSPSEVVVYSESLLSLSGDNEHV