AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63655

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63655.t1 MTR_3g034180 88.141 312 37 0 1 312 178 489 0.0 575
MS.gene63655.t1 MTR_3g034130 87.625 299 36 1 1 298 64 362 0.0 537
MS.gene63655.t1 MTR_3g034160 85.256 312 44 2 1 311 64 374 0.0 529
MS.gene63655.t1 MTR_3g103020 64.013 314 97 7 3 311 75 377 6.48e-129 372
MS.gene63655.t1 MTR_4g117720 63.158 323 92 12 1 311 70 377 5.03e-123 357
MS.gene63655.t1 MTR_6g060650 62.112 322 100 7 1 311 62 372 3.73e-121 352
MS.gene63655.t1 MTR_4g109280 63.754 309 94 7 6 311 65 358 8.94e-114 333
MS.gene63655.t1 MTR_4g109270 60.063 318 102 9 1 311 64 363 3.98e-113 332
MS.gene63655.t1 MTR_4g122850 55.591 313 123 9 6 312 62 364 2.38e-104 310
MS.gene63655.t1 MTR_4g117850 57.190 306 107 9 7 305 85 373 1.19e-102 305
MS.gene63655.t1 MTR_4g117770 55.627 311 117 7 9 312 71 367 7.34e-99 295
MS.gene63655.t1 MTR_4g117810 55.627 311 117 7 9 312 71 367 7.34e-99 295
MS.gene63655.t1 MTR_4g118380 56.291 302 99 7 6 285 67 357 2.32e-98 294
MS.gene63655.t1 MTR_4g094365 53.292 319 125 9 1 312 63 364 2.16e-97 291
MS.gene63655.t1 MTR_4g117780 53.205 312 123 8 9 312 71 367 4.83e-95 286
MS.gene63655.t1 MTR_4g023080 53.503 314 119 9 1 311 64 353 1.21e-92 279
MS.gene63655.t1 MTR_4g116330 53.376 311 123 8 8 311 84 379 3.18e-92 279
MS.gene63655.t1 MTR_5g097210 51.475 305 131 7 7 309 71 360 7.59e-92 278
MS.gene63655.t1 MTR_4g023090 49.550 333 130 9 1 312 71 386 5.94e-91 276
MS.gene63655.t1 MTR_4g021785 51.911 314 125 8 1 311 64 354 3.86e-90 273
MS.gene63655.t1 MTR_4g117750 51.899 316 120 9 7 311 64 358 1.12e-89 276
MS.gene63655.t1 MTR_4g117750 61.468 109 39 2 205 310 373 481 7.08e-34 129
MS.gene63655.t1 MTR_5g070080 49.697 330 129 7 6 311 69 385 1.64e-89 272
MS.gene63655.t1 MTR_4g023380 48.680 341 114 11 1 312 71 379 8.43e-86 263
MS.gene63655.t1 MTR_5g070130 49.840 313 133 9 6 306 70 370 1.34e-85 262
MS.gene63655.t1 MTR_4g117870 50.000 314 108 8 1 312 1 267 7.14e-85 256
MS.gene63655.t1 MTR_1g055135 48.408 314 141 9 7 311 73 374 3.62e-83 256
MS.gene63655.t1 MTR_4g023070 48.171 328 137 11 1 311 58 369 1.35e-81 251
MS.gene63655.t1 MTR_1g055195 48.265 317 132 8 2 309 182 475 1.68e-81 255
MS.gene63655.t1 MTR_4g023190 49.527 317 129 10 1 310 65 357 2.16e-81 251
MS.gene63655.t1 MTR_4g023130 54.982 271 97 8 44 311 64 312 1.89e-80 246
MS.gene63655.t1 MTR_4g023200 48.265 317 134 10 1 310 65 358 2.74e-80 248
MS.gene63655.t1 MTR_8g433040 50.000 314 119 11 9 312 79 364 1.16e-79 246
MS.gene63655.t1 MTR_8g032380 50.000 314 119 11 9 312 79 364 1.16e-79 246
MS.gene63655.t1 MTR_4g023120 48.024 329 138 12 1 312 58 370 2.02e-79 246
MS.gene63655.t1 MTR_5g070088 46.386 332 142 10 6 312 66 386 2.23e-79 246
MS.gene63655.t1 MTR_4g023150 48.276 319 131 11 1 310 65 358 2.01e-78 243
MS.gene63655.t1 MTR_4g116320 59.821 224 74 7 91 309 43 255 1.04e-76 235
MS.gene63655.t1 MTR_4g023440 47.284 313 110 9 1 312 74 332 3.30e-76 236
MS.gene63655.t1 MTR_8g032390 47.452 314 108 11 7 306 99 369 6.39e-74 232
MS.gene63655.t1 MTR_4g118400 47.333 300 111 8 16 306 33 294 8.02e-72 224
MS.gene63655.t1 MTR_8g013010 60.185 216 69 6 1 214 109 309 3.52e-71 223
MS.gene63655.t1 MTR_4g118370 46.429 280 117 7 33 309 8 257 6.72e-70 218
MS.gene63655.t1 MTR_7g079400 43.182 308 151 9 14 311 43 336 6.37e-68 215
MS.gene63655.t1 MTR_8g040935 56.604 212 79 5 107 311 2 207 1.69e-67 210
MS.gene63655.t1 MTR_7g079370 43.711 318 148 11 7 307 48 351 1.34e-65 210
MS.gene63655.t1 MTR_4g116330 45.016 311 110 9 8 311 84 340 1.94e-63 204
MS.gene63655.t1 MTR_8g433030 54.425 226 83 8 87 306 125 336 1.31e-62 202
MS.gene63655.t1 MTR_4g121580 47.249 309 106 16 7 302 75 339 6.93e-59 194
MS.gene63655.t1 MTR_1g055165 41.100 309 117 9 7 311 70 317 9.67e-56 183
MS.gene63655.t1 MTR_8g102520 41.139 316 133 12 7 311 48 321 1.50e-54 181
MS.gene63655.t1 MTR_6g463430 50.000 210 88 6 109 312 4 202 7.51e-52 169
MS.gene63655.t1 MTR_1g055175 50.000 200 87 7 118 311 8 200 5.30e-51 167
MS.gene63655.t1 MTR_4g117430 52.299 174 60 5 46 217 3 155 2.47e-49 161
MS.gene63655.t1 MTR_4g117530 39.792 289 113 10 28 311 20 252 3.47e-49 164
MS.gene63655.t1 MTR_1g055250 43.290 231 115 9 74 299 34 253 4.23e-49 166
MS.gene63655.t1 MTR_2g048580 35.390 308 154 11 10 311 73 341 1.09e-47 163
MS.gene63655.t1 MTR_4g114700 41.264 269 96 7 42 303 5 218 2.07e-47 159
MS.gene63655.t1 MTR_4g116340 49.721 179 74 5 7 180 80 247 9.12e-44 150
MS.gene63655.t1 MTR_4g118360 61.417 127 42 4 190 311 1 125 1.97e-41 140
MS.gene63655.t1 MTR_7g079420 36.462 277 120 9 45 307 905 1139 4.96e-40 149
MS.gene63655.t1 MTR_8g098830 44.509 173 83 4 114 281 30 194 1.50e-39 139
MS.gene63655.t1 MTR_4g122860 43.367 196 74 6 123 312 156 320 1.74e-36 134
MS.gene63655.t1 MTR_5g023000 68.478 92 28 1 221 311 7 98 7.68e-34 120
MS.gene63655.t1 MTR_7g117720 53.425 146 52 7 7 147 52 186 4.96e-33 123
MS.gene63655.t1 MTR_7g117720 52.688 93 39 2 220 307 180 272 1.69e-16 78.6
MS.gene63655.t1 MTR_6g034720 45.860 157 64 6 40 193 31 169 7.75e-30 113
MS.gene63655.t1 MTR_7g079450 42.667 150 60 6 174 311 1 136 9.58e-25 97.4
MS.gene63655.t1 MTR_5g069120 45.736 129 61 2 6 127 51 177 4.62e-23 94.4
MS.gene63655.t1 MTR_1g068875 27.843 255 155 11 3 243 52 291 6.70e-22 95.1
MS.gene63655.t1 MTR_4g122840 43.704 135 39 3 181 312 164 264 2.49e-21 91.7
MS.gene63655.t1 MTR_4g133170 30.180 222 126 11 32 238 78 285 3.73e-20 90.1
MS.gene63655.t1 MTR_0667s0010 26.014 296 170 11 4 268 57 334 2.97e-19 87.8
MS.gene63655.t1 MTR_2g024110 26.515 264 160 11 10 259 63 306 1.56e-18 85.5
MS.gene63655.t1 MTR_2g426020 26.087 276 162 10 31 281 88 346 2.59e-18 85.1
MS.gene63655.t1 MTR_2g028160 27.899 276 155 12 21 268 86 345 4.08e-18 84.7
MS.gene63655.t1 MTR_2g031640 27.556 225 138 8 33 242 81 295 6.56e-18 83.6
MS.gene63655.t1 MTR_2g026010 29.386 228 136 9 32 242 78 297 7.91e-18 83.6
MS.gene63655.t1 MTR_1g068835 27.686 242 144 11 19 243 65 292 1.39e-17 82.8
MS.gene63655.t1 MTR_4g023430 56.098 82 32 2 235 312 141 222 2.43e-17 79.7
MS.gene63655.t1 MTR_2g025960 25.185 270 161 9 39 281 1 256 4.80e-17 80.5
MS.gene63655.t1 MTR_1g068860 25.316 237 150 8 21 243 71 294 7.19e-17 80.5
MS.gene63655.t1 MTR_8g013005 67.241 58 18 1 255 311 1 58 9.91e-17 73.6
MS.gene63655.t1 MTR_3g083640 29.500 200 110 8 44 238 157 330 9.96e-17 80.5
MS.gene63655.t1 MTR_2g426070 29.954 217 123 9 55 259 127 326 1.00e-16 80.5
MS.gene63655.t1 MTR_1g055200 48.039 102 44 4 42 139 5 101 1.58e-16 74.3
MS.gene63655.t1 MTR_2g028130 27.600 250 143 10 37 261 100 336 1.76e-16 79.7
MS.gene63655.t1 MTR_6g065980 31.313 198 107 9 44 238 111 282 7.63e-16 77.4
MS.gene63655.t1 MTR_4g031770 29.545 220 124 11 31 242 93 289 9.69e-16 77.4
MS.gene63655.t1 MTR_2g078870 31.500 200 107 9 44 238 58 232 1.24e-15 76.3
MS.gene63655.t1 MTR_2g021155 29.891 184 113 5 59 238 711 882 1.57e-15 77.8
MS.gene63655.t1 MTR_2g021155 30.435 230 133 11 22 242 110 321 8.67e-12 66.2
MS.gene63655.t1 MTR_2g426090 26.609 233 136 9 29 238 86 306 1.63e-15 77.0
MS.gene63655.t1 MTR_4g055060 29.435 248 143 10 9 243 94 322 2.99e-15 75.9
MS.gene63655.t1 MTR_2g425980 30.435 207 113 9 54 242 119 312 4.42e-15 75.5
MS.gene63655.t1 MTR_2g031720 23.151 311 193 11 2 279 43 340 4.84e-15 75.5
MS.gene63655.t1 MTR_3g010870 26.459 257 142 9 32 281 122 338 6.58e-15 75.1
MS.gene63655.t1 MTR_4g070550 27.311 238 134 9 34 243 92 318 8.32e-15 74.7
MS.gene63655.t1 MTR_6g053240 27.143 210 123 7 41 238 125 316 1.68e-14 73.9
MS.gene63655.t1 MTR_2g031680 26.222 225 140 8 32 242 80 292 1.98e-14 73.6
MS.gene63655.t1 MTR_2g028140 26.866 268 139 12 25 259 91 334 2.03e-14 73.6
MS.gene63655.t1 MTR_2g025420 25.000 292 168 12 6 261 67 343 3.78e-14 72.8
MS.gene63655.t1 MTR_3g006670 29.167 216 129 9 31 238 133 332 4.22e-14 72.8
MS.gene63655.t1 MTR_7g070440 26.786 280 156 12 16 264 69 330 4.62e-14 72.4
MS.gene63655.t1 MTR_8g464770 27.010 311 185 15 17 306 88 377 8.24e-14 71.6
MS.gene63655.t1 MTR_3g007330 27.962 211 121 7 44 243 138 328 8.38e-14 71.6
MS.gene63655.t1 MTR_8g099460 24.231 260 162 8 4 242 56 301 2.08e-13 70.5
MS.gene63655.t1 MTR_3g007410 28.436 211 127 8 44 245 146 341 2.15e-13 70.5
MS.gene63655.t1 MTR_4g061340 29.108 213 122 6 44 243 120 316 2.35e-13 70.5
MS.gene63655.t1 MTR_5g011200 28.019 207 126 9 40 240 114 303 2.41e-13 70.1
MS.gene63655.t1 MTR_3g007360 28.169 213 125 8 44 245 146 341 3.12e-13 70.1
MS.gene63655.t1 MTR_5g037680 24.920 313 190 11 13 304 93 381 3.79e-13 70.1
MS.gene63655.t1 MTR_1g094210 30.116 259 135 14 40 283 151 378 4.54e-13 69.7
MS.gene63655.t1 MTR_7g070830 28.455 246 123 13 56 264 146 375 1.07e-12 68.6
MS.gene63655.t1 MTR_3g036430 25.248 202 116 8 44 238 96 269 1.15e-12 68.2
MS.gene63655.t1 MTR_7g056390 29.412 238 114 11 41 251 130 340 1.30e-12 68.2
MS.gene63655.t1 MTR_4g023100 64.912 57 17 2 257 312 65 119 1.58e-12 63.9
MS.gene63655.t1 MTR_2g078900 30.220 182 100 8 59 238 153 309 2.33e-12 67.4
MS.gene63655.t1 MTR_5g046280 27.230 213 120 9 44 246 3 190 2.59e-12 66.2
MS.gene63655.t1 MTR_6g053260 28.708 209 128 7 34 238 429 620 2.61e-12 67.8
MS.gene63655.t1 MTR_5g046300 27.230 213 120 9 44 246 100 287 2.90e-12 67.0
MS.gene63655.t1 MTR_5g046290 27.230 213 120 9 44 246 100 287 2.90e-12 67.0
MS.gene63655.t1 MTR_2g064960 68.293 41 13 0 271 311 18 58 4.06e-12 60.8
MS.gene63655.t1 MTR_3g007430 27.907 215 128 8 35 238 126 324 4.90e-12 66.2
MS.gene63655.t1 MTR_4g109290 64.151 53 18 1 260 311 135 187 4.97e-12 64.3
MS.gene63655.t1 MTR_3g009310 28.421 190 115 7 56 243 157 327 5.01e-12 66.2
MS.gene63655.t1 MTR_1g056710 26.840 231 143 8 24 242 102 318 6.00e-12 66.2
MS.gene63655.t1 MTR_0129s0070 25.155 322 202 13 17 312 91 399 6.25e-12 66.2
MS.gene63655.t1 MTR_5g068490 24.460 278 178 10 20 289 5 258 6.74e-12 65.1
MS.gene63655.t1 MTR_4g117550 27.302 315 174 14 17 310 94 374 7.60e-12 65.9
MS.gene63655.t1 MTR_6g027910 26.148 283 179 9 38 304 106 374 8.50e-12 65.5
MS.gene63655.t1 MTR_3g014540 29.268 205 118 9 44 238 124 311 1.45e-11 65.1
MS.gene63655.t1 MTR_7g017040 24.465 327 200 13 16 310 88 399 1.51e-11 65.1
MS.gene63655.t1 MTR_5g057710 30.583 206 110 11 44 238 143 326 1.56e-11 65.1
MS.gene63655.t1 MTR_7g057270 26.506 249 139 10 31 247 107 343 1.56e-11 65.1
MS.gene63655.t1 MTR_2g078850 29.703 202 113 8 44 238 130 309 1.70e-11 64.7
MS.gene63655.t1 MTR_5g022040 26.400 250 136 12 23 247 166 392 1.76e-11 65.1
MS.gene63655.t1 MTR_7g056290 28.632 234 121 10 41 251 134 344 2.08e-11 64.7
MS.gene63655.t1 MTR_6g065510 28.302 212 117 9 54 249 71 263 2.13e-11 64.3
MS.gene63655.t1 MTR_4g108820 28.448 232 134 9 30 255 96 301 2.55e-11 63.9
MS.gene63655.t1 MTR_3g436150 28.125 256 144 12 44 287 135 362 2.56e-11 64.3
MS.gene63655.t1 MTR_2g043410 26.087 276 151 11 40 283 133 387 4.20e-11 63.5
MS.gene63655.t1 MTR_3g024130 29.302 215 119 11 41 243 58 251 4.26e-11 63.2
MS.gene63655.t1 MTR_6g004660 27.076 277 161 12 18 283 95 341 4.98e-11 63.5
MS.gene63655.t1 MTR_5g086590 26.106 226 132 9 54 270 11 210 6.18e-11 62.0
MS.gene63655.t1 MTR_4g030830 25.676 222 116 11 44 238 113 312 7.14e-11 62.8
MS.gene63655.t1 MTR_4g007940 26.493 268 141 13 57 290 78 323 9.11e-11 62.4
MS.gene63655.t1 MTR_3g010590 26.761 213 120 9 32 238 124 306 9.69e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63655.t1 AT3G06240 29.060 234 128 12 39 259 163 371 4.43e-17 81.6
MS.gene63655.t1 AT4G12560 29.249 253 160 10 40 275 90 340 6.23e-16 78.2
MS.gene63655.t1 AT4G12560 29.249 253 160 10 40 275 90 340 6.23e-16 78.2
MS.gene63655.t1 AT4G12560 29.249 253 160 10 40 275 90 340 6.23e-16 78.2
MS.gene63655.t1 AT4G22390 25.641 273 169 12 32 281 82 343 6.70e-11 63.2
MS.gene63655.t1 AT4G22390 25.641 273 169 12 32 281 82 343 7.32e-11 63.2

Find 70 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAAATGTGGTCATATTAT+TGG 0.222453 3.2:-23082601 MS.gene63655:CDS
TCATGTTGTCTGTATGGTTT+TGG 0.229471 3.2:-23083129 MS.gene63655:CDS
TGAATATTGGCCATCTAAAT+TGG 0.231444 3.2:+23083149 None:intergenic
GATTGTGATTTGTGGGTATT+TGG 0.240897 3.2:-23082769 MS.gene63655:CDS
TTGGAGAATTTCTGAGTCTA+TGG 0.272887 3.2:-23082750 MS.gene63655:CDS
ACTGTTGTCCTTTCTATTGA+TGG 0.275628 3.2:-23082658 MS.gene63655:CDS
TCAAGATTAGAATAAGATTC+AGG 0.281222 3.2:+23083276 None:intergenic
GTTCTTCAAGCATTTATCTA+TGG 0.293765 3.2:-23083215 MS.gene63655:CDS
AAGCGATGCTTCAAAATCTA+TGG 0.328942 3.2:+23083344 None:intergenic
AGATAATATTTCATCACATT+TGG 0.336831 3.2:-23083037 MS.gene63655:CDS
TTTCCTCCTTCCAATTTAGA+TGG 0.347200 3.2:-23083159 MS.gene63655:CDS
CATACAGACAACATGAATAT+TGG 0.369761 3.2:+23083136 None:intergenic
TATTGTTGGCTTTGATTTAG+TGG 0.370118 3.2:-23082845 MS.gene63655:CDS
CCATTTGCATTAATATAATA+CGG 0.370854 3.2:+23082943 None:intergenic
GATGAAATATTATCTTCAAT+TGG 0.372589 3.2:+23083045 None:intergenic
CAATATTCATGTTGTCTGTA+TGG 0.390156 3.2:-23083135 MS.gene63655:CDS
GGGGTGCAACAATTGAAATA+TGG 0.394607 3.2:-23082720 MS.gene63655:CDS
CCGTATTATATTAATGCAAA+TGG 0.412595 3.2:-23082943 MS.gene63655:CDS
TGTTTGTGATGTCCGGTTGA+TGG 0.414503 3.2:+23083189 None:intergenic
ATGAAATATTATCTTCAATT+GGG 0.422682 3.2:+23083046 None:intergenic
ATTATTGGAACAAATGGTAC+TGG 0.443784 3.2:-23082586 MS.gene63655:CDS
CCCTTCAAGAGATGATTACT+TGG 0.451416 3.2:-23083100 MS.gene63655:CDS
AGGTATTTGTTTGTGATGTC+CGG 0.452958 3.2:+23083182 None:intergenic
AATCACAATCTATAGGTTCA+TGG 0.456461 3.2:+23082782 None:intergenic
GGTTCATGGTCAAAACCATC+TGG 0.456773 3.2:+23082796 None:intergenic
TGGGTCTATACTTGTATCAT+AGG 0.457138 3.2:+23083065 None:intergenic
GGTCATATTATTGGAACAAA+TGG 0.461469 3.2:-23082592 MS.gene63655:CDS
TCTGAGTCTATGGGCTATGG+GGG 0.467239 3.2:-23082740 MS.gene63655:CDS
TCTAAATTGGAAGGAGGAAA+AGG 0.467688 3.2:+23083162 None:intergenic
ATTTCTGAGTCTATGGGCTA+TGG 0.468799 3.2:-23082743 MS.gene63655:CDS
ACCAAGTAATCATCTCTTGA+AGG 0.476545 3.2:+23083099 None:intergenic
TGCAAATACCATCAATAGAA+AGG 0.476953 3.2:+23082650 None:intergenic
TTATCAATGGATGTTATTGT+TGG 0.479970 3.2:-23082859 MS.gene63655:CDS
ACCCACAAATCACAATCTAT+AGG 0.488895 3.2:+23082775 None:intergenic
TTTCTGAGTCTATGGGCTAT+GGG 0.490757 3.2:-23082742 MS.gene63655:CDS
CTCTGTATACATAGCCACTA+GGG 0.491428 3.2:+23082495 None:intergenic
TGAGTATACTTCACCAATCC+AGG 0.492432 3.2:+23082970 None:intergenic
AATACAAAGTGCATTCGTCT+TGG 0.494278 3.2:-23082687 MS.gene63655:CDS
AACCTATAGATTGTGATTTG+TGG 0.495657 3.2:-23082777 MS.gene63655:CDS
TTCTGAGTCTATGGGCTATG+GGG 0.499186 3.2:-23082741 MS.gene63655:CDS
ACCTATAGATTGTGATTTGT+GGG 0.500615 3.2:-23082776 MS.gene63655:CDS
TGGAAAGAAATTGAGTGTCC+TGG 0.509163 3.2:-23082988 MS.gene63655:CDS
TATCTATGGAATCCATCAAC+CGG 0.509941 3.2:-23083201 MS.gene63655:CDS
TGGAGAATTTCTGAGTCTAT+GGG 0.513573 3.2:-23082749 MS.gene63655:CDS
TAAATTTGCTCATTGTCACT+AGG 0.515439 3.2:+23082457 None:intergenic
AAATTTGCTCATTGTCACTA+GGG 0.519922 3.2:+23082458 None:intergenic
GGGTGCAACAATTGAAATAT+GGG 0.520467 3.2:-23082719 MS.gene63655:CDS
GCAAATGGTGATCCCAAAGT+AGG 0.520873 3.2:-23082928 MS.gene63655:CDS
ATTCAGGAGGCATAAAATCA+AGG 0.527920 3.2:+23083292 None:intergenic
TTTCATTGAGAGCTAATACA+TGG 0.531728 3.2:-23083008 MS.gene63655:CDS
CATTTGCATTAATATAATAC+GGG 0.537071 3.2:+23082944 None:intergenic
GAAATTAAAAGTTCATGTAG+AGG 0.538733 3.2:-23083255 MS.gene63655:CDS
TTCAAGAGATGATTACTTGG+TGG 0.549809 3.2:-23083097 MS.gene63655:CDS
TGGAACAAATGGTACTGGAT+TGG 0.564323 3.2:-23082581 MS.gene63655:CDS
TTGGCTTTGATTTAGTGGAA+AGG 0.565737 3.2:-23082840 MS.gene63655:CDS
TATTGGCCATCTAAATTGGA+AGG 0.567363 3.2:+23083153 None:intergenic
AGAAATTGAGTGTCCTGGAT+TGG 0.569095 3.2:-23082983 MS.gene63655:CDS
CCAATATGCTCTACAAAATG+TGG 0.574609 3.2:-23082613 MS.gene63655:CDS
CAAATGGTGATCCCAAAGTA+GGG 0.574981 3.2:-23082927 MS.gene63655:CDS
TGAAGTAGAAGCAGAATCAT+CGG 0.584397 3.2:+23083320 None:intergenic
CTGAGTCTATGGGCTATGGG+GGG 0.597580 3.2:-23082739 MS.gene63655:CDS
CCAAGTAATCATCTCTTGAA+GGG 0.615000 3.2:+23083100 None:intergenic
ACTCTGTATACATAGCCACT+AGG 0.618200 3.2:+23082494 None:intergenic
TGGCCATCTAAATTGGAAGG+AGG 0.651013 3.2:+23083156 None:intergenic
CGAACCATGTGGACCCCTAG+TGG 0.655781 3.2:-23082509 MS.gene63655:CDS
ATAGCCACTAGGGGTCCACA+TGG 0.664631 3.2:+23082505 None:intergenic
AGATTAGAATAAGATTCAGG+AGG 0.666008 3.2:+23083279 None:intergenic
TCTGTATACATAGCCACTAG+GGG 0.698983 3.2:+23082496 None:intergenic
TCATACTATAGCGAACCATG+TGG 0.753194 3.2:-23082520 MS.gene63655:CDS
TTGGTGAAGTATGATGACAA+TGG 0.788821 3.2:-23082562 MS.gene63655:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAAATATTATCTTCAATT+GGG + chr3.2:23082785-23082804 None:intergenic 15.0%
!! AGATAATATTTCATCACATT+TGG - chr3.2:23082791-23082810 MS.gene63655:CDS 20.0%
!! CATTTGCATTAATATAATAC+GGG + chr3.2:23082887-23082906 None:intergenic 20.0%
!! CCATTTGCATTAATATAATA+CGG + chr3.2:23082888-23082907 None:intergenic 20.0%
!! GATGAAATATTATCTTCAAT+TGG + chr3.2:23082786-23082805 None:intergenic 20.0%
! ACAAAATGTGGTCATATTAT+TGG - chr3.2:23083227-23083246 MS.gene63655:CDS 25.0%
! CCGTATTATATTAATGCAAA+TGG - chr3.2:23082885-23082904 MS.gene63655:CDS 25.0%
! GAAATTAAAAGTTCATGTAG+AGG - chr3.2:23082573-23082592 MS.gene63655:CDS 25.0%
! TCAAGATTAGAATAAGATTC+AGG + chr3.2:23082555-23082574 None:intergenic 25.0%
! TTATCAATGGATGTTATTGT+TGG - chr3.2:23082969-23082988 MS.gene63655:CDS 25.0%
!! TTTTCGCAATGATTTATCAA+TGG - chr3.2:23082956-23082975 MS.gene63655:CDS 25.0%
!! TTTTTAATGGGACTATTCAT+TGG - chr3.2:23082928-23082947 MS.gene63655:CDS 25.0%
AACCTATAGATTGTGATTTG+TGG - chr3.2:23083051-23083070 MS.gene63655:CDS 30.0%
AATCACAATCTATAGGTTCA+TGG + chr3.2:23083049-23083068 None:intergenic 30.0%
AGATTAGAATAAGATTCAGG+AGG + chr3.2:23082552-23082571 None:intergenic 30.0%
ATTATTGGAACAAATGGTAC+TGG - chr3.2:23083242-23083261 MS.gene63655:CDS 30.0%
CAATATTCATGTTGTCTGTA+TGG - chr3.2:23082693-23082712 MS.gene63655:CDS 30.0%
CATACAGACAACATGAATAT+TGG + chr3.2:23082695-23082714 None:intergenic 30.0%
GGTCATATTATTGGAACAAA+TGG - chr3.2:23083236-23083255 MS.gene63655:CDS 30.0%
GTTCTTCAAGCATTTATCTA+TGG - chr3.2:23082613-23082632 MS.gene63655:CDS 30.0%
TGAATATTGGCCATCTAAAT+TGG + chr3.2:23082682-23082701 None:intergenic 30.0%
TGCAAATACCATCAATAGAA+AGG + chr3.2:23083181-23083200 None:intergenic 30.0%
TTTCATTGAGAGCTAATACA+TGG - chr3.2:23082820-23082839 MS.gene63655:CDS 30.0%
! AAATTTGCTCATTGTCACTA+GGG + chr3.2:23083373-23083392 None:intergenic 30.0%
! ACATTTTGTAGAGCATATTG+GGG + chr3.2:23083216-23083235 None:intergenic 30.0%
! ACCTATAGATTGTGATTTGT+GGG - chr3.2:23083052-23083071 MS.gene63655:CDS 30.0%
! CACATTTTGTAGAGCATATT+GGG + chr3.2:23083217-23083236 None:intergenic 30.0%
! TAAATTTGCTCATTGTCACT+AGG + chr3.2:23083374-23083393 None:intergenic 30.0%
!! TATTGTTGGCTTTGATTTAG+TGG - chr3.2:23082983-23083002 MS.gene63655:CDS 30.0%
AAGCGATGCTTCAAAATCTA+TGG + chr3.2:23082487-23082506 None:intergenic 35.0%
AATACAAAGTGCATTCGTCT+TGG - chr3.2:23083141-23083160 MS.gene63655:CDS 35.0%
ACCAAGTAATCATCTCTTGA+AGG + chr3.2:23082732-23082751 None:intergenic 35.0%
ACCCACAAATCACAATCTAT+AGG + chr3.2:23083056-23083075 None:intergenic 35.0%
ACTGTTGTCCTTTCTATTGA+TGG - chr3.2:23083170-23083189 MS.gene63655:CDS 35.0%
AGGTATTTGTTTGTGATGTC+CGG + chr3.2:23082649-23082668 None:intergenic 35.0%
CCAAGTAATCATCTCTTGAA+GGG + chr3.2:23082731-23082750 None:intergenic 35.0%
CCAATATGCTCTACAAAATG+TGG - chr3.2:23083215-23083234 MS.gene63655:CDS 35.0%
GGGTGCAACAATTGAAATAT+GGG - chr3.2:23083109-23083128 MS.gene63655:CDS 35.0%
TATCTATGGAATCCATCAAC+CGG - chr3.2:23082627-23082646 MS.gene63655:CDS 35.0%
TATTGGCCATCTAAATTGGA+AGG + chr3.2:23082678-23082697 None:intergenic 35.0%
TCTAAATTGGAAGGAGGAAA+AGG + chr3.2:23082669-23082688 None:intergenic 35.0%
TGAAGTAGAAGCAGAATCAT+CGG + chr3.2:23082511-23082530 None:intergenic 35.0%
TGGAGAATTTCTGAGTCTAT+GGG - chr3.2:23083079-23083098 MS.gene63655:CDS 35.0%
TGGGTCTATACTTGTATCAT+AGG + chr3.2:23082766-23082785 None:intergenic 35.0%
TTCAAGAGATGATTACTTGG+TGG - chr3.2:23082731-23082750 MS.gene63655:CDS 35.0%
TTGGAGAATTTCTGAGTCTA+TGG - chr3.2:23083078-23083097 MS.gene63655:CDS 35.0%
TTGGTGAAGTATGATGACAA+TGG - chr3.2:23083266-23083285 MS.gene63655:CDS 35.0%
TTTCCTCCTTCCAATTTAGA+TGG - chr3.2:23082669-23082688 MS.gene63655:CDS 35.0%
! ATTCAGGAGGCATAAAATCA+AGG + chr3.2:23082539-23082558 None:intergenic 35.0%
! CATGTTGTCTGTATGGTTTT+GGG - chr3.2:23082700-23082719 MS.gene63655:CDS 35.0%
! CATTTTGTAGAGCATATTGG+GGG + chr3.2:23083215-23083234 None:intergenic 35.0%
! CCACATTTTGTAGAGCATAT+TGG + chr3.2:23083218-23083237 None:intergenic 35.0%
! GATTGTGATTTGTGGGTATT+TGG - chr3.2:23083059-23083078 MS.gene63655:CDS 35.0%
! TCATGTTGTCTGTATGGTTT+TGG - chr3.2:23082699-23082718 MS.gene63655:CDS 35.0%
! TTAGAGATGCATTTTCCAGA+TGG - chr3.2:23083017-23083036 MS.gene63655:CDS 35.0%
!! AAAGTAGGGACGCTTTTTAA+TGG - chr3.2:23082915-23082934 MS.gene63655:CDS 35.0%
!! AAGTAGGGACGCTTTTTAAT+GGG - chr3.2:23082916-23082935 MS.gene63655:CDS 35.0%
!! ATTAAAAAGCGTCCCTACTT+TGG + chr3.2:23082916-23082935 None:intergenic 35.0%
!! TTAAAAAGCGTCCCTACTTT+GGG + chr3.2:23082915-23082934 None:intergenic 35.0%
!! TTGGCTTTGATTTAGTGGAA+AGG - chr3.2:23082988-23083007 MS.gene63655:CDS 35.0%
ACTCTGTATACATAGCCACT+AGG + chr3.2:23083337-23083356 None:intergenic 40.0%
AGAAATTGAGTGTCCTGGAT+TGG - chr3.2:23082845-23082864 MS.gene63655:CDS 40.0%
CAAATGGTGATCCCAAAGTA+GGG - chr3.2:23082901-23082920 MS.gene63655:CDS 40.0%
CCCTTCAAGAGATGATTACT+TGG - chr3.2:23082728-23082747 MS.gene63655:CDS 40.0%
CTCTGTATACATAGCCACTA+GGG + chr3.2:23083336-23083355 None:intergenic 40.0%
GGGGTGCAACAATTGAAATA+TGG - chr3.2:23083108-23083127 MS.gene63655:CDS 40.0%
TCATACTATAGCGAACCATG+TGG - chr3.2:23083308-23083327 MS.gene63655:CDS 40.0%
TCTGTATACATAGCCACTAG+GGG + chr3.2:23083335-23083354 None:intergenic 40.0%
TGAGTATACTTCACCAATCC+AGG + chr3.2:23082861-23082880 None:intergenic 40.0%
TGGAAAGAAATTGAGTGTCC+TGG - chr3.2:23082840-23082859 MS.gene63655:CDS 40.0%
! ATTTCTGAGTCTATGGGCTA+TGG - chr3.2:23083085-23083104 MS.gene63655:CDS 40.0%
! TGGAACAAATGGTACTGGAT+TGG - chr3.2:23083247-23083266 MS.gene63655:CDS 40.0%
! TTTCTGAGTCTATGGGCTAT+GGG - chr3.2:23083086-23083105 MS.gene63655:CDS 40.0%
GCAAATGGTGATCCCAAAGT+AGG - chr3.2:23082900-23082919 MS.gene63655:CDS 45.0%
GGTTCATGGTCAAAACCATC+TGG + chr3.2:23083035-23083054 None:intergenic 45.0%
TGGCCATCTAAATTGGAAGG+AGG + chr3.2:23082675-23082694 None:intergenic 45.0%
TGTTTGTGATGTCCGGTTGA+TGG + chr3.2:23082642-23082661 None:intergenic 45.0%
! TTCTGAGTCTATGGGCTATG+GGG - chr3.2:23083087-23083106 MS.gene63655:CDS 45.0%
! TCTGAGTCTATGGGCTATGG+GGG - chr3.2:23083088-23083107 MS.gene63655:CDS 50.0%
ATAGCCACTAGGGGTCCACA+TGG + chr3.2:23083326-23083345 None:intergenic 55.0%
! CTGAGTCTATGGGCTATGGG+GGG - chr3.2:23083089-23083108 MS.gene63655:CDS 55.0%
CGAACCATGTGGACCCCTAG+TGG - chr3.2:23083319-23083338 MS.gene63655:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 23082456 23083394 23082456 ID=MS.gene63655
chr3.2 mRNA 23082456 23083394 23082456 ID=MS.gene63655.t1;Parent=MS.gene63655
chr3.2 exon 23082456 23083394 23082456 ID=MS.gene63655.t1.exon1;Parent=MS.gene63655.t1
chr3.2 CDS 23082456 23083394 23082456 ID=cds.MS.gene63655.t1;Parent=MS.gene63655.t1
Gene Sequence

>MS.gene63655

ATGTCAACTCTAGCTCTTGAAACTCGATCCATAGATTTTGAAGCATCGCTTACCGATGATTCTGCTTCTACTTCACTGAACCTTGATTTTATGCCTCCTGAATCTTATTCTAATCTTGAAATTAAAAGTTCATGTAGAGGATTTATAGTTTTGACTTGTTCTTCAAGCATTTATCTATGGAATCCATCAACCGGACATCACAAACAAATACCTTTTCCTCCTTCCAATTTAGATGGCCAATATTCATGTTGTCTGTATGGTTTTGGGTACGACCCTTCAAGAGATGATTACTTGGTGGTATCAGTGTCCTATGATACAAGTATAGACCCAATTGAAGATAATATTTCATCACATTTGGAGTTTTTTTCATTGAGAGCTAATACATGGAAAGAAATTGAGTGTCCTGGATTGGTGAAGTATACTCACTTCCCGTATTATATTAATGCAAATGGTGATCCCAAAGTAGGGACGCTTTTTAATGGGACTATTCATTGGTTCTCTTTTCGCAATGATTTATCAATGGATGTTATTGTTGGCTTTGATTTAGTGGAAAGGAAACTTTTAGAGATGCATTTTCCAGATGGTTTTGACCATGAACCTATAGATTGTGATTTGTGGGTATTTGGAGAATTTCTGAGTCTATGGGCTATGGGGGGTGCAACAATTGAAATATGGGTTATGAAAGAATACAAAGTGCATTCGTCTTGGACTAAGACTGTTGTCCTTTCTATTGATGGTATTTGCATTCAATATGATCCCCCAATATGCTCTACAAAATGTGGTCATATTATTGGAACAAATGGTACTGGATTGGTGAAGTATGATGACAATGGTCAGTTGCTAGAGAATCGCTCATACTATAGCGAACCATGTGGACCCCTAGTGGCTATGTATACAGAGTCTCTGCTTTCACTCCCTAGTGACAATGAGCAAATTTAA
Protein sequence

>MS.gene63655.t1

MSTLALETRSIDFEASLTDDSASTSLNLDFMPPESYSNLEIKSSCRGFIVLTCSSSIYLWNPSTGHHKQIPFPPSNLDGQYSCCLYGFGYDPSRDDYLVVSVSYDTSIDPIEDNISSHLEFFSLRANTWKEIECPGLVKYTHFPYYINANGDPKVGTLFNGTIHWFSFRNDLSMDVIVGFDLVERKLLEMHFPDGFDHEPIDCDLWVFGEFLSLWAMGGATIEIWVMKEYKVHSSWTKTVVLSIDGICIQYDPPICSTKCGHIIGTNGTGLVKYDDNGQLLENRSYYSEPCGPLVAMYTESLLSLPSDNEQI