AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58722


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58722.t1 MTR_4g109270 62.963 135 48 1 13 147 232 364 1.72e-49 162
MS.gene58722.t1 MTR_8g433040 60.000 135 49 2 13 147 235 364 9.56e-45 150
MS.gene58722.t1 MTR_8g032380 60.000 135 49 2 13 147 235 364 9.56e-45 150
MS.gene58722.t1 MTR_3g103020 61.481 135 50 1 13 147 246 378 4.24e-44 149
MS.gene58722.t1 MTR_4g094365 57.664 137 53 2 13 147 231 364 1.52e-43 147
MS.gene58722.t1 MTR_4g116330 56.934 137 55 2 13 147 246 380 1.37e-42 145
MS.gene58722.t1 MTR_8g433030 60.305 131 46 3 13 142 212 337 1.41e-42 144
MS.gene58722.t1 MTR_8g032390 60.305 131 46 3 13 142 245 370 3.64e-42 144
MS.gene58722.t1 MTR_8g040935 57.037 135 54 2 15 147 76 208 4.51e-42 139
MS.gene58722.t1 MTR_4g109280 59.854 137 48 4 13 147 228 359 3.49e-41 141
MS.gene58722.t1 MTR_4g117780 62.281 114 41 1 34 147 256 367 5.23e-41 140
MS.gene58722.t1 MTR_4g117770 62.281 114 41 1 34 147 256 367 5.52e-41 140
MS.gene58722.t1 MTR_4g117810 62.281 114 41 1 34 147 256 367 5.52e-41 140
MS.gene58722.t1 MTR_4g116320 57.463 134 51 2 13 144 126 255 9.43e-41 137
MS.gene58722.t1 MTR_6g060650 56.716 134 54 2 13 146 243 372 1.48e-40 139
MS.gene58722.t1 MTR_4g117530 66.038 106 31 2 42 147 153 253 2.26e-40 136
MS.gene58722.t1 MTR_4g122850 56.835 139 54 4 13 149 232 366 2.11e-39 137
MS.gene58722.t1 MTR_4g023130 56.716 134 53 3 13 146 184 312 4.80e-39 134
MS.gene58722.t1 MTR_5g023000 65.000 100 33 2 48 147 2 99 1.65e-37 124
MS.gene58722.t1 MTR_4g023080 53.731 134 57 3 13 146 225 353 7.61e-37 129
MS.gene58722.t1 MTR_4g117850 57.480 127 51 2 15 140 249 373 9.90e-37 129
MS.gene58722.t1 MTR_4g117870 52.593 135 60 2 13 147 137 267 3.18e-36 125
MS.gene58722.t1 MTR_4g118370 52.273 132 57 2 13 144 132 257 3.47e-36 125
MS.gene58722.t1 MTR_4g117720 56.028 141 52 5 13 147 242 378 3.52e-36 128
MS.gene58722.t1 MTR_4g023380 49.405 168 52 6 9 147 216 379 4.66e-36 128
MS.gene58722.t1 MTR_3g034180 56.716 134 53 3 13 146 360 488 4.47e-35 127
MS.gene58722.t1 MTR_1g055175 50.746 134 61 3 15 147 72 201 6.08e-35 120
MS.gene58722.t1 MTR_1g055195 51.079 139 60 3 13 149 348 480 4.91e-34 124
MS.gene58722.t1 MTR_1g055165 52.593 135 57 4 15 147 189 318 9.54e-34 120
MS.gene58722.t1 MTR_4g117750 58.559 111 44 1 35 145 373 481 4.24e-33 122
MS.gene58722.t1 MTR_4g117750 54.464 112 45 2 35 146 253 358 1.01e-30 115
MS.gene58722.t1 MTR_5g097210 54.264 129 54 2 15 143 236 359 5.04e-33 120
MS.gene58722.t1 MTR_4g118360 57.391 115 41 3 35 147 18 126 7.86e-33 113
MS.gene58722.t1 MTR_4g023070 49.650 143 59 3 15 146 229 369 1.11e-32 119
MS.gene58722.t1 MTR_4g021785 49.630 135 63 3 13 147 226 355 1.63e-32 118
MS.gene58722.t1 MTR_4g023190 53.676 136 57 5 13 145 225 357 3.23e-32 117
MS.gene58722.t1 MTR_4g116330 56.522 115 46 2 35 147 229 341 3.67e-32 117
MS.gene58722.t1 MTR_6g463430 47.059 136 64 2 13 147 74 202 7.02e-32 113
MS.gene58722.t1 MTR_4g023090 48.299 147 58 4 13 145 240 382 8.51e-32 117
MS.gene58722.t1 MTR_4g118400 65.909 88 28 1 54 141 209 294 3.55e-31 113
MS.gene58722.t1 MTR_4g122860 50.000 128 59 3 22 147 196 320 5.07e-31 114
MS.gene58722.t1 MTR_3g034160 57.463 134 52 3 13 146 246 374 2.04e-30 113
MS.gene58722.t1 MTR_4g023120 48.611 144 61 4 15 147 229 370 3.54e-30 112
MS.gene58722.t1 MTR_5g070080 53.333 135 57 4 15 147 256 386 4.47e-30 112
MS.gene58722.t1 MTR_4g023150 51.471 136 60 5 13 145 226 358 6.49e-30 111
MS.gene58722.t1 MTR_4g023200 51.471 136 60 5 13 145 226 358 9.97e-30 111
MS.gene58722.t1 MTR_4g023440 59.184 98 36 2 50 147 239 332 2.01e-29 109
MS.gene58722.t1 MTR_5g070130 48.951 143 69 3 13 153 243 383 1.25e-28 108
MS.gene58722.t1 MTR_1g055135 46.429 140 65 4 13 147 241 375 1.47e-28 108
MS.gene58722.t1 MTR_4g118380 52.137 117 43 3 13 120 245 357 2.24e-28 107
MS.gene58722.t1 MTR_3g034130 52.101 119 53 2 15 133 248 362 7.95e-28 106
MS.gene58722.t1 MTR_7g079450 51.485 101 44 2 48 147 41 137 1.42e-26 97.4
MS.gene58722.t1 MTR_5g070088 48.092 131 65 2 13 142 252 380 5.64e-26 101
MS.gene58722.t1 MTR_8g102520 49.505 101 46 2 48 147 226 322 1.94e-23 93.6
MS.gene58722.t1 MTR_1g055250 47.107 121 55 5 15 134 141 253 3.98e-22 90.5
MS.gene58722.t1 MTR_4g114700 56.180 89 34 2 53 138 132 218 1.52e-21 87.4
MS.gene58722.t1 MTR_8g013005 71.186 59 17 0 89 147 1 59 1.71e-21 82.4
MS.gene58722.t1 MTR_7g079400 46.479 142 55 9 17 147 206 337 3.62e-20 85.1
MS.gene58722.t1 MTR_7g117720 58.889 90 31 4 58 143 186 273 9.38e-20 83.2
MS.gene58722.t1 MTR_4g122840 40.909 132 40 3 17 147 170 264 3.11e-19 81.6
MS.gene58722.t1 MTR_7g079370 52.688 93 39 2 50 141 262 350 2.42e-18 80.5
MS.gene58722.t1 MTR_4g121580 44.715 123 54 4 16 137 230 339 1.64e-17 78.2
MS.gene58722.t1 MTR_4g023430 39.860 143 64 5 15 147 92 222 2.60e-17 75.9
MS.gene58722.t1 MTR_8g098830 47.115 104 42 5 17 116 100 194 1.01e-16 74.3
MS.gene58722.t1 MTR_7g079420 40.580 138 66 6 17 147 1016 1144 7.45e-16 73.9
MS.gene58722.t1 MTR_2g048580 45.263 95 40 3 53 147 260 342 2.58e-14 68.9
MS.gene58722.t1 MTR_4g109290 66.038 53 18 0 95 147 136 188 3.77e-14 66.6
MS.gene58722.t1 MTR_4g023100 60.000 55 20 1 92 146 66 118 6.25e-12 59.3
MS.gene58722.t1 MTR_2g064960 69.048 42 13 0 106 147 18 59 6.69e-12 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTCTGGTTTGTGGGTATT+TGG 0.119331 8.1:+9884467 MS.gene58722:CDS
ATTCTGGTTTGTGGGTATTT+GGG 0.224908 8.1:+9884468 MS.gene58722:CDS
GACAATATACTTGACTATTC+TGG 0.288946 8.1:+9884452 MS.gene58722:CDS
ATAATCATACGGTTGAAATA+TGG 0.292170 8.1:+9884522 MS.gene58722:CDS
CTCATTAACATTGGCATCAA+TGG 0.363458 8.1:-9884597 None:intergenic
CCATACGGTTTGTGGGTTTG+TGG 0.390139 8.1:+9884386 MS.gene58722:CDS
ATTGGAACATGTGGTGGTAT+TGG 0.396640 8.1:+9884665 MS.gene58722:CDS
TACTTGACTATTCTGGTTTG+TGG 0.398497 8.1:+9884459 MS.gene58722:CDS
AATACAAATTGCATTCATCT+TGG 0.398909 8.1:+9884555 MS.gene58722:CDS
AAGTAAAACCATACGGTTTG+TGG 0.415808 8.1:+9884378 MS.gene58722:CDS
TAATCATACGGTTGAAATAT+GGG 0.438296 8.1:+9884523 MS.gene58722:CDS
AGTATATTGTCAAACTCATC+TGG 0.441674 8.1:-9884440 None:intergenic
ACTTGACTATTCTGGTTTGT+GGG 0.445951 8.1:+9884460 MS.gene58722:CDS
CATACGGTTTGTGGGTTTGT+GGG 0.446555 8.1:+9884387 MS.gene58722:CDS
TTCTGGTTTGTGGGTATTTG+GGG 0.446634 8.1:+9884469 MS.gene58722:CDS
TGTGGGTTTGTGGGTTAACA+AGG 0.452247 8.1:+9884396 MS.gene58722:CDS
GAGTCTCTGCTCTCACTCCC+TGG 0.513546 8.1:+9884776 MS.gene58722:CDS
GGGATTCACGTGAATCGTTA+CGG 0.517791 8.1:-9884736 None:intergenic
AGTAAAACCATACGGTTTGT+GGG 0.523582 8.1:+9884379 MS.gene58722:CDS
AACAAGCTTTAAGAAGAATG+AGG 0.543856 8.1:+9884807 MS.gene58722:CDS
CTCTGTATACATAGCCACTT+GGG 0.546267 8.1:-9884756 None:intergenic
AAAGCTTGTTCACTTTCACC+AGG 0.551123 8.1:-9884794 None:intergenic
CCACAAACCCACAAACCGTA+TGG 0.565600 8.1:-9884386 None:intergenic
AGAAAGAGACTCATTAACAT+TGG 0.571014 8.1:-9884606 None:intergenic
AACATGTGGTGGTATTGGAT+TGG 0.578026 8.1:+9884670 MS.gene58722:CDS
AAGCTTGTTCACTTTCACCA+GGG 0.600758 8.1:-9884793 None:intergenic
ACTCTGTATACATAGCCACT+TGG 0.613352 8.1:-9884757 None:intergenic
GGTGATATTATTGGAACATG+TGG 0.633288 8.1:+9884656 MS.gene58722:CDS
GATATTATTGGAACATGTGG+TGG 0.648140 8.1:+9884659 MS.gene58722:CDS
ACGATGCAAGTAAAACCATA+CGG 0.652244 8.1:+9884371 None:intergenic
TTGGTGAAGTACAATGACAA+AGG 0.668246 8.1:+9884689 MS.gene58722:CDS
CGATTCACGTGAATCCCAAG+TGG 0.676953 8.1:+9884742 MS.gene58722:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATAATTTTGATAATCATA+CGG + chr8.1:9884511-9884530 MS.gene58722:CDS 10.0%
! AATACAAATTGCATTCATCT+TGG + chr8.1:9884555-9884574 MS.gene58722:CDS 25.0%
! ATAATCATACGGTTGAAATA+TGG + chr8.1:9884522-9884541 MS.gene58722:CDS 25.0%
! TAATCATACGGTTGAAATAT+GGG + chr8.1:9884523-9884542 MS.gene58722:CDS 25.0%
AGAAAGAGACTCATTAACAT+TGG - chr8.1:9884609-9884628 None:intergenic 30.0%
AGTATATTGTCAAACTCATC+TGG - chr8.1:9884443-9884462 None:intergenic 30.0%
GACAATATACTTGACTATTC+TGG + chr8.1:9884452-9884471 MS.gene58722:CDS 30.0%
GCAAAAAGTGGTGATATTAT+TGG + chr8.1:9884647-9884666 MS.gene58722:CDS 30.0%
!! AACAAGCTTTAAGAAGAATG+AGG + chr8.1:9884807-9884826 MS.gene58722:CDS 30.0%
AAGTAAAACCATACGGTTTG+TGG + chr8.1:9884378-9884397 MS.gene58722:CDS 35.0%
AGTAAAACCATACGGTTTGT+GGG + chr8.1:9884379-9884398 MS.gene58722:CDS 35.0%
CTCATTAACATTGGCATCAA+TGG - chr8.1:9884600-9884619 None:intergenic 35.0%
GATATTATTGGAACATGTGG+TGG + chr8.1:9884659-9884678 MS.gene58722:CDS 35.0%
GGTGATATTATTGGAACATG+TGG + chr8.1:9884656-9884675 MS.gene58722:CDS 35.0%
TTGGTGAAGTACAATGACAA+AGG + chr8.1:9884689-9884708 MS.gene58722:CDS 35.0%
!! ACTTGACTATTCTGGTTTGT+GGG + chr8.1:9884460-9884479 MS.gene58722:CDS 35.0%
!! ATTCTGGTTTGTGGGTATTT+GGG + chr8.1:9884468-9884487 MS.gene58722:CDS 35.0%
!! TACTTGACTATTCTGGTTTG+TGG + chr8.1:9884459-9884478 MS.gene58722:CDS 35.0%
!! TATTCTGGTTTGTGGGTATT+TGG + chr8.1:9884467-9884486 MS.gene58722:CDS 35.0%
ACTCTGTATACATAGCCACT+TGG - chr8.1:9884760-9884779 None:intergenic 40.0%
ATTGGAACATGTGGTGGTAT+TGG + chr8.1:9884665-9884684 MS.gene58722:CDS 40.0%
CTCTGTATACATAGCCACTT+GGG - chr8.1:9884759-9884778 None:intergenic 40.0%
! AAAGCTTGTTCACTTTCACC+AGG - chr8.1:9884797-9884816 None:intergenic 40.0%
! AACATGTGGTGGTATTGGAT+TGG + chr8.1:9884670-9884689 MS.gene58722:CDS 40.0%
! AAGCTTGTTCACTTTCACCA+GGG - chr8.1:9884796-9884815 None:intergenic 40.0%
! ACTTTTTGCACAGCACAATG+GGG - chr8.1:9884636-9884655 None:intergenic 40.0%
!! CACTTTTTGCACAGCACAAT+GGG - chr8.1:9884637-9884656 None:intergenic 40.0%
!! TTCTGGTTTGTGGGTATTTG+GGG + chr8.1:9884469-9884488 MS.gene58722:CDS 40.0%
CATACGGTTTGTGGGTTTGT+GGG + chr8.1:9884387-9884406 MS.gene58722:CDS 45.0%
CCATTGTGCTGTGCAAAAAG+TGG + chr8.1:9884635-9884654 MS.gene58722:CDS 45.0%
GGGATTCACGTGAATCGTTA+CGG - chr8.1:9884739-9884758 None:intergenic 45.0%
! TGTGGGTTTGTGGGTTAACA+AGG + chr8.1:9884396-9884415 MS.gene58722:CDS 45.0%
!!! CCACTTTTTGCACAGCACAA+TGG - chr8.1:9884638-9884657 None:intergenic 45.0%
CCACAAACCCACAAACCGTA+TGG - chr8.1:9884389-9884408 None:intergenic 50.0%
CCATACGGTTTGTGGGTTTG+TGG + chr8.1:9884386-9884405 MS.gene58722:CDS 50.0%
CGATTCACGTGAATCCCAAG+TGG + chr8.1:9884742-9884761 MS.gene58722:CDS 50.0%
GAGTCTCTGCTCTCACTCCC+TGG + chr8.1:9884776-9884795 MS.gene58722:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 9884374 9884835 9884374 ID=MS.gene58722
chr8.1 mRNA 9884374 9884835 9884374 ID=MS.gene58722.t1;Parent=MS.gene58722
chr8.1 exon 9884374 9884835 9884374 ID=MS.gene58722.t1.exon1;Parent=MS.gene58722.t1
chr8.1 CDS 9884374 9884835 9884374 ID=cds.MS.gene58722.t1;Parent=MS.gene58722.t1
Gene Sequence

>MS.gene58722

ATGCAAGTAAAACCATACGGTTTGTGGGTTTGTGGGTTAACAAGGAAACTTTGCAATATGTATTTGCCAGATGAGTTTGACAATATACTTGACTATTCTGGTTTGTGGGTATTTGGGGAATTTCTCAGTTTTTCGTCTAATAATTTTGATAATCATACGGTTGAAATATGGGTGATGAAAGAATACAAATTGCATTCATCTTGGACAAAGACTCATGTTCTGTCCATTGATGCCAATGTTAATGAGTCTCTTTCTTTTTCCCCATTGTGCTGTGCAAAAAGTGGTGATATTATTGGAACATGTGGTGGTATTGGATTGGTGAAGTACAATGACAAAGGACAGCTACTAGAGCATTGCTCTTACCGTAACGATTCACGTGAATCCCAAGTGGCTATGTATACAGAGTCTCTGCTCTCACTCCCTGGTGAAAGTGAACAAGCTTTAAGAAGAATGAGGTTGTGA

Protein sequence

>MS.gene58722.t1

MQVKPYGLWVCGLTRKLCNMYLPDEFDNILDYSGLWVFGEFLSFSSNNFDNHTVEIWVMKEYKLHSSWTKTHVLSIDANVNESLSFSPLCCAKSGDIIGTCGGIGLVKYNDKGQLLEHCSYRNDSRESQVAMYTESLLSLPGESEQALRRMRL