AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50329

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50329.t1 MTR_4g023120 77.151 372 48 3 1 337 1 370 0.0 569
MS.gene50329.t1 MTR_4g023070 76.819 371 49 3 1 336 1 369 0.0 563
MS.gene50329.t1 MTR_4g023090 58.730 378 106 7 3 337 16 386 2.67e-140 402
MS.gene50329.t1 MTR_4g023080 59.189 370 90 6 3 336 9 353 4.04e-138 396
MS.gene50329.t1 MTR_4g021785 57.527 372 96 6 3 337 9 355 6.56e-137 393
MS.gene50329.t1 MTR_4g023380 58.721 344 77 8 25 337 70 379 2.15e-122 357
MS.gene50329.t1 MTR_4g023130 58.457 337 104 7 3 336 9 312 5.34e-121 351
MS.gene50329.t1 MTR_4g023150 53.908 371 111 7 3 335 10 358 8.44e-111 327
MS.gene50329.t1 MTR_4g023200 57.325 314 107 6 27 335 67 358 1.55e-110 326
MS.gene50329.t1 MTR_4g023190 53.639 371 111 8 3 335 10 357 1.96e-107 318
MS.gene50329.t1 MTR_3g103020 51.194 377 124 10 3 337 20 378 2.22e-107 318
MS.gene50329.t1 MTR_4g023440 50.538 372 90 6 3 337 18 332 1.55e-104 310
MS.gene50329.t1 MTR_4g117720 50.000 350 136 13 1 334 49 375 3.02e-95 288
MS.gene50329.t1 MTR_4g094365 46.702 379 136 11 3 337 8 364 7.18e-91 276
MS.gene50329.t1 MTR_3g034180 46.615 384 139 13 3 337 123 489 1.94e-90 279
MS.gene50329.t1 MTR_4g109280 46.667 375 137 11 3 337 8 359 3.23e-90 274
MS.gene50329.t1 MTR_4g116330 45.789 380 133 12 3 334 23 377 7.27e-89 271
MS.gene50329.t1 MTR_3g034160 46.316 380 140 11 3 334 9 372 7.07e-88 269
MS.gene50329.t1 MTR_3g034130 47.802 364 123 12 3 317 9 354 9.58e-88 268
MS.gene50329.t1 MTR_4g109270 46.234 385 133 12 1 337 6 364 3.16e-87 266
MS.gene50329.t1 MTR_4g117850 46.032 378 134 13 3 334 23 376 6.02e-87 266
MS.gene50329.t1 MTR_8g433040 45.383 379 131 11 3 337 18 364 2.20e-85 262
MS.gene50329.t1 MTR_8g032380 45.383 379 131 11 3 337 18 364 2.20e-85 262
MS.gene50329.t1 MTR_4g117770 44.180 378 147 10 3 337 11 367 3.08e-81 251
MS.gene50329.t1 MTR_4g117810 44.180 378 147 10 3 337 11 367 3.08e-81 251
MS.gene50329.t1 MTR_4g117780 43.799 379 149 10 2 337 10 367 5.12e-81 251
MS.gene50329.t1 MTR_6g060650 45.337 386 138 14 3 336 8 372 5.98e-81 251
MS.gene50329.t1 MTR_4g122850 46.257 374 130 16 14 337 12 364 1.34e-79 248
MS.gene50329.t1 MTR_8g032390 44.474 371 125 11 3 332 40 370 1.45e-78 245
MS.gene50329.t1 MTR_1g055135 41.775 383 155 12 3 337 13 375 8.24e-74 233
MS.gene50329.t1 MTR_5g097210 42.933 375 148 12 3 334 9 360 1.16e-73 232
MS.gene50329.t1 MTR_4g117870 45.338 311 110 11 34 337 10 267 5.93e-70 219
MS.gene50329.t1 MTR_5g070130 40.691 376 158 10 3 330 11 369 6.63e-70 223
MS.gene50329.t1 MTR_8g433030 41.962 367 107 10 3 332 40 337 6.90e-70 221
MS.gene50329.t1 MTR_4g118380 43.243 370 130 12 3 312 8 357 5.44e-69 219
MS.gene50329.t1 MTR_5g070080 40.152 396 150 14 3 334 10 382 1.04e-67 217
MS.gene50329.t1 MTR_5g070088 38.228 395 164 10 3 335 7 383 1.17e-67 217
MS.gene50329.t1 MTR_4g121580 40.838 382 134 14 3 336 11 348 7.42e-67 215
MS.gene50329.t1 MTR_4g116330 40.526 380 114 12 3 334 23 338 1.80e-66 213
MS.gene50329.t1 MTR_4g116320 51.082 231 88 6 111 334 43 255 5.16e-60 193
MS.gene50329.t1 MTR_1g055195 40.426 329 149 10 22 334 178 475 2.75e-59 198
MS.gene50329.t1 MTR_6g463430 51.869 214 80 5 130 337 6 202 5.16e-59 189
MS.gene50329.t1 MTR_4g117750 41.207 381 150 12 1 333 1 355 1.03e-56 191
MS.gene50329.t1 MTR_4g117750 51.240 121 43 3 219 335 373 481 9.06e-25 104
MS.gene50329.t1 MTR_8g013010 43.542 271 97 8 1 228 52 309 4.39e-56 185
MS.gene50329.t1 MTR_4g118400 39.053 338 144 12 3 330 8 293 1.76e-55 183
MS.gene50329.t1 MTR_1g055165 36.533 375 132 11 3 337 10 318 2.42e-54 181
MS.gene50329.t1 MTR_8g040935 49.296 213 85 6 135 337 9 208 9.10e-53 173
MS.gene50329.t1 MTR_7g079370 38.820 322 157 11 30 330 47 349 1.40e-50 172
MS.gene50329.t1 MTR_4g118370 41.288 264 107 11 80 334 33 257 2.32e-47 160
MS.gene50329.t1 MTR_7g079400 38.333 300 147 11 54 337 60 337 6.74e-47 162
MS.gene50329.t1 MTR_4g117530 35.799 338 107 10 3 337 23 253 4.84e-44 152
MS.gene50329.t1 MTR_4g116340 43.779 217 91 6 3 193 36 247 5.48e-43 149
MS.gene50329.t1 MTR_8g102520 34.444 360 166 17 3 337 8 322 1.40e-41 147
MS.gene50329.t1 MTR_7g117720 50.829 181 64 5 3 162 10 186 2.52e-41 145
MS.gene50329.t1 MTR_7g117720 47.222 108 37 4 231 330 175 270 1.77e-17 81.6
MS.gene50329.t1 MTR_1g055175 43.062 209 92 8 139 336 8 200 2.84e-39 138
MS.gene50329.t1 MTR_1g055250 38.400 250 118 10 101 337 44 270 3.44e-38 139
MS.gene50329.t1 MTR_4g114700 39.416 274 87 9 68 327 9 217 1.10e-37 135
MS.gene50329.t1 MTR_4g117430 51.316 152 57 6 79 227 14 151 8.91e-37 130
MS.gene50329.t1 MTR_2g048580 30.890 382 156 17 3 330 8 335 3.56e-35 131
MS.gene50329.t1 MTR_4g023430 48.462 130 37 4 216 337 115 222 2.13e-27 107
MS.gene50329.t1 MTR_7g079420 31.944 288 128 14 67 335 905 1143 2.07e-24 104
MS.gene50329.t1 MTR_4g122860 36.634 202 79 9 146 337 158 320 7.07e-24 100
MS.gene50329.t1 MTR_5g023000 51.818 110 37 3 232 337 2 99 1.55e-23 93.6
MS.gene50329.t1 MTR_4g118360 49.600 125 41 5 216 334 15 123 2.62e-23 93.6
MS.gene50329.t1 MTR_1g094210 27.056 377 190 16 3 329 57 398 2.84e-22 97.1
MS.gene50329.t1 MTR_1g094210 26.685 356 177 15 3 309 57 377 4.37e-22 96.7
MS.gene50329.t1 MTR_2g055970 26.882 372 205 13 3 329 211 560 5.43e-22 97.1
MS.gene50329.t1 MTR_6g034720 49.505 101 47 2 105 204 72 169 8.47e-22 92.4
MS.gene50329.t1 MTR_1g097490 27.049 366 204 13 3 329 49 390 4.46e-21 93.6
MS.gene50329.t1 MTR_8g098830 36.517 178 86 9 140 308 35 194 2.31e-20 88.6
MS.gene50329.t1 MTR_1g097560 27.224 371 195 19 2 329 35 373 3.52e-20 90.5
MS.gene50329.t1 MTR_4g023100 76.364 55 13 0 283 337 65 119 4.40e-20 84.7
MS.gene50329.t1 MTR_1g097330 28.205 312 176 10 3 275 16 318 5.39e-20 90.5
MS.gene50329.t1 MTR_2g021050 26.596 376 194 16 2 333 4 341 1.28e-19 89.0
MS.gene50329.t1 MTR_2g046920 29.217 332 169 17 3 283 24 340 4.00e-19 87.8
MS.gene50329.t1 MTR_4g108820 29.630 270 155 11 3 258 42 290 9.51e-19 85.9
MS.gene50329.t1 MTR_4g133170 27.987 318 177 15 2 276 3 311 1.15e-18 86.3
MS.gene50329.t1 MTR_8g467140 28.289 304 156 13 3 259 32 320 2.10e-18 85.1
MS.gene50329.t1 MTR_5g022420 28.213 319 163 14 3 275 27 325 5.72e-18 84.3
MS.gene50329.t1 MTR_7g079450 36.538 156 75 7 187 337 1 137 2.31e-17 77.8
MS.gene50329.t1 MTR_4g107820 25.532 376 207 16 2 329 35 385 7.57e-17 81.3
MS.gene50329.t1 MTR_4g107780 25.532 376 207 16 2 329 35 385 7.57e-17 81.3
MS.gene50329.t1 MTR_4g107780 25.532 376 207 16 2 329 35 385 1.01e-16 80.9
MS.gene50329.t1 MTR_2g021150 27.164 335 177 17 3 290 14 328 1.07e-16 80.5
MS.gene50329.t1 MTR_4g107820 25.532 376 207 16 2 329 35 385 1.24e-16 80.5
MS.gene50329.t1 MTR_0667s0010 26.347 334 173 15 3 275 6 327 2.79e-16 79.7
MS.gene50329.t1 MTR_2g046900 26.409 337 182 16 3 290 28 347 3.21e-16 79.3
MS.gene50329.t1 MTR_8g013005 64.286 56 18 1 283 336 3 58 4.34e-16 72.0
MS.gene50329.t1 MTR_1g097370 25.667 300 155 13 3 290 68 311 8.86e-16 78.6
MS.gene50329.t1 MTR_3g036290 29.795 292 139 14 3 252 22 289 2.00e-15 76.6
MS.gene50329.t1 MTR_2g426090 25.767 326 175 13 6 271 9 327 2.39e-15 76.6
MS.gene50329.t1 MTR_1g068835 25.862 348 197 15 2 306 3 332 4.69e-15 75.9
MS.gene50329.t1 MTR_7g445890 25.191 393 206 16 3 331 16 384 8.03e-15 75.1
MS.gene50329.t1 MTR_2g425980 25.635 394 204 19 2 328 4 375 9.72e-15 75.1
MS.gene50329.t1 MTR_1g068875 26.625 323 176 15 2 276 3 312 1.77e-14 73.9
MS.gene50329.t1 MTR_2g021155 26.667 285 140 13 3 270 671 903 2.30e-14 74.7
MS.gene50329.t1 MTR_2g021155 26.923 338 187 17 6 302 39 357 2.72e-13 71.2
MS.gene50329.t1 MTR_1g050542 24.251 367 218 18 3 329 58 404 2.34e-14 73.9
MS.gene50329.t1 MTR_0128s0020 26.000 400 197 17 3 328 18 392 2.46e-14 73.6
MS.gene50329.t1 MTR_2g025420 25.501 349 173 16 3 275 6 343 2.94e-14 73.6
MS.gene50329.t1 MTR_7g062100 25.441 397 206 14 3 331 24 398 3.95e-14 73.2
MS.gene50329.t1 MTR_2g024110 25.705 319 179 13 2 272 3 311 6.79e-14 72.4
MS.gene50329.t1 MTR_5g057790 29.712 313 151 16 3 266 46 338 7.69e-14 72.4
MS.gene50329.t1 MTR_7g070440 27.213 305 161 14 5 256 9 305 8.60e-14 72.0
MS.gene50329.t1 MTR_2g031680 25.000 316 186 13 3 275 6 313 9.16e-14 72.0
MS.gene50329.t1 MTR_3g007430 29.110 292 149 14 6 252 46 324 1.18e-13 71.6
MS.gene50329.t1 MTR_4g013180 26.966 356 174 17 3 290 11 348 1.27e-13 72.0
MS.gene50329.t1 MTR_2g024100 28.713 202 103 10 78 252 119 306 1.59e-13 71.2
MS.gene50329.t1 MTR_7g060960 29.936 314 156 17 3 276 32 321 1.69e-13 70.9
MS.gene50329.t1 MTR_2g028110 25.387 323 181 14 2 275 3 314 1.70e-13 71.2
MS.gene50329.t1 MTR_2g031640 26.101 318 183 12 3 275 6 316 1.97e-13 70.9
MS.gene50329.t1 MTR_5g069120 40.952 105 53 1 31 126 52 156 2.14e-13 68.2
MS.gene50329.t1 MTR_5g096710 27.525 396 202 19 3 331 23 400 2.25e-13 70.9
MS.gene50329.t1 MTR_3g013770 26.110 383 205 15 3 332 33 390 2.96e-13 70.5
MS.gene50329.t1 MTR_8g099460 25.325 308 169 12 1 256 3 301 3.81e-13 70.1
MS.gene50329.t1 MTR_7g062060 24.138 406 213 16 3 330 12 400 4.26e-13 70.1
MS.gene50329.t1 MTR_7g057270 26.519 362 175 16 3 290 26 370 4.71e-13 70.1
MS.gene50329.t1 MTR_4g117550 25.442 283 142 10 77 335 137 374 6.14e-13 69.3
MS.gene50329.t1 MTR_1g055200 43.434 99 46 3 64 156 5 99 6.17e-13 64.7
MS.gene50329.t1 MTR_3g014540 27.321 377 202 19 3 332 29 380 7.22e-13 69.3
MS.gene50329.t1 MTR_7g070830 26.844 339 159 14 3 259 21 352 1.13e-12 68.9
MS.gene50329.t1 MTR_6g027910 24.675 385 207 16 6 329 12 374 1.16e-12 68.6
MS.gene50329.t1 MTR_3g014570 25.269 372 217 13 3 332 29 381 1.24e-12 68.6
MS.gene50329.t1 MTR_7g070880 25.676 370 178 15 3 290 21 375 1.29e-12 68.6
MS.gene50329.t1 MTR_0044s0300 27.444 266 155 11 76 329 150 389 1.30e-12 68.6
MS.gene50329.t1 MTR_6g021880 25.602 332 212 12 12 329 71 381 1.52e-12 68.2
MS.gene50329.t1 MTR_5g057690 28.115 313 146 14 3 266 46 328 2.00e-12 67.8
MS.gene50329.t1 MTR_7g078860 25.895 363 176 15 3 290 9 353 2.82e-12 67.4
MS.gene50329.t1 MTR_4g061340 28.763 299 151 15 3 252 26 311 2.90e-12 67.4
MS.gene50329.t1 MTR_3g010390 25.325 308 171 12 3 256 33 335 3.19e-12 67.4
MS.gene50329.t1 MTR_5g086590 31.088 193 116 10 74 256 9 194 3.23e-12 65.9
MS.gene50329.t1 MTR_2g461450 27.586 290 154 14 3 252 18 291 3.40e-12 67.0
MS.gene50329.t1 MTR_3g009300 27.068 266 157 10 6 252 37 284 3.97e-12 67.0
MS.gene50329.t1 MTR_3g023930 24.259 371 200 15 3 329 34 367 4.45e-12 66.6
MS.gene50329.t1 MTR_6g017295 27.333 300 165 16 29 290 5 289 5.88e-12 67.0
MS.gene50329.t1 MTR_6g065510 26.923 312 159 15 3 290 23 289 7.93e-12 65.9
MS.gene50329.t1 MTR_5g057710 29.206 315 148 17 3 265 46 337 8.24e-12 66.2
MS.gene50329.t1 MTR_3g007360 27.415 383 186 21 6 330 55 403 9.95e-12 65.9
MS.gene50329.t1 MTR_1g088120 23.677 359 190 11 6 292 13 359 1.03e-11 65.9
MS.gene50329.t1 MTR_2g104340 24.069 403 219 15 3 336 14 398 1.61e-11 65.1
MS.gene50329.t1 MTR_8g046200 29.752 242 118 11 60 256 122 356 2.10e-11 65.1
MS.gene50329.t1 MTR_4g109290 64.151 53 17 1 287 337 136 188 2.21e-11 62.4
MS.gene50329.t1 MTR_6g065980 24.538 379 198 17 3 329 16 358 2.94e-11 64.3
MS.gene50329.t1 MTR_6g027890 26.007 273 157 12 81 329 73 324 3.44e-11 63.9
MS.gene50329.t1 MTR_4g028750 27.014 211 119 8 69 252 127 329 4.17e-11 63.9
MS.gene50329.t1 MTR_3g007390 32.026 153 87 7 105 252 1 141 4.33e-11 62.0
MS.gene50329.t1 MTR_2g026010 27.615 239 143 11 61 275 86 318 5.11e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50329.t1 AT4G12560 29.412 238 143 10 60 274 88 323 6.84e-17 81.6
MS.gene50329.t1 AT4G12560 29.412 238 143 10 60 274 88 323 6.84e-17 81.6
MS.gene50329.t1 AT4G12560 29.412 238 143 10 60 274 88 323 6.84e-17 81.6
MS.gene50329.t1 AT3G23880 25.625 320 171 14 2 272 13 314 6.20e-12 66.2
MS.gene50329.t1 AT3G06240 25.795 283 170 10 61 329 163 419 1.94e-11 65.1

Find 74 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGAATGGATTACATTTAT+TGG 0.150019 4.4:+78221529 None:intergenic
ACAGAAAATGGTGATATTAT+TGG 0.164193 4.4:-78221047 MS.gene50329:CDS
CATTCTGGTTTGTGGGTATT+TGG 0.166612 4.4:-78221251 MS.gene50329:CDS
GCTCTGTGGTCAACATTATT+TGG 0.173886 4.4:+78221281 None:intergenic
CATTGTTGGCTTTGATTTAA+TGG 0.174382 4.4:-78221330 MS.gene50329:CDS
TTCCCTTATGTCGTTGATTA+TGG 0.178480 4.4:-78221425 MS.gene50329:CDS
GTGAAAGTAGTCCGCATTTC+TGG 0.200250 4.4:+78221082 None:intergenic
AAATCAATTGATCGAATTTC+AGG 0.224377 4.4:+78221809 None:intergenic
AAAACATATATTACTCTATA+TGG 0.230356 4.4:-78221599 MS.gene50329:CDS
GGTGACAATGAGCATGTTTA+AGG 0.231011 4.4:-78220903 None:intergenic
TTGGAGAATTTCTCAGTATA+TGG 0.239627 4.4:-78221232 MS.gene50329:CDS
CCTATATATTCTACAGAAAA+TGG 0.261164 4.4:-78221059 MS.gene50329:CDS
TATATTACTCTATATGGTTT+TGG 0.286814 4.4:-78221593 MS.gene50329:CDS
ATAATGATACAATTGAAATA+TGG 0.287461 4.4:-78221196 MS.gene50329:CDS
TCAAATTCTGTTGAGGTTAC+CGG 0.316216 4.4:-78222588 MS.gene50329:CDS
ATATAGAGTAATATATGTTT+TGG 0.322465 4.4:+78221601 None:intergenic
GGTGATATTATTGGAAAATA+TGG 0.325508 4.4:-78221038 MS.gene50329:CDS
CACAGAGCATTAGTACATTC+TGG 0.337046 4.4:-78221266 MS.gene50329:CDS
GGATTCCATATGTAGATGTT+TGG 0.388278 4.4:+78221668 None:intergenic
TCTCCATAATCAACGACATA+AGG 0.405779 4.4:+78221422 None:intergenic
TGGAGAATTTCTCAGTATAT+GGG 0.411388 4.4:-78221231 MS.gene50329:CDS
ATTTCTCAGTATATGGGTTA+AGG 0.418550 4.4:-78221225 MS.gene50329:CDS
TAATGGGGCTATTCATTGGT+TGG 0.421138 4.4:-78221381 MS.gene50329:CDS
ATTAGTACATTCTGGTTTGT+GGG 0.428082 4.4:-78221258 MS.gene50329:CDS
CATTAGTACATTCTGGTTTG+TGG 0.428815 4.4:-78221259 MS.gene50329:CDS
CATGACCCTTTAATTTCAAC+GGG 0.433411 4.4:+78221719 None:intergenic
CAGTTATAGTTCGGTGAAGC+AGG 0.435410 4.4:+78221764 None:intergenic
AAATGTAATCCATTCTTAGT+TGG 0.444016 4.4:-78221524 MS.gene50329:CDS
CTCCATAATCAACGACATAA+GGG 0.469919 4.4:+78221423 None:intergenic
TAATGATACAATTGAAATAT+GGG 0.471725 4.4:-78221195 MS.gene50329:CDS
TCTCGTTTAGAGACAATACT+TGG 0.472530 4.4:-78221469 MS.gene50329:CDS
ATAGCTTTGGATGCCATTGT+TGG 0.473343 4.4:-78221344 MS.gene50329:CDS
TTCGCGTCGTGATATAGCTT+TGG 0.477282 4.4:-78221357 MS.gene50329:CDS
GTCATCCAAACATCTACATA+TGG 0.477819 4.4:-78221673 MS.gene50329:CDS
TCAACGGGAAAATAAGAGTC+TGG 0.484289 4.4:+78221734 None:intergenic
GTAAAACCACCAACTAAGAA+TGG 0.485862 4.4:+78221515 None:intergenic
GCATTTCTGGAATAATATAG+AGG 0.489061 4.4:+78221095 None:intergenic
TGTAATCCATTCTTAGTTGG+TGG 0.495212 4.4:-78221521 MS.gene50329:CDS
AGAGGGTTAAAGTAGTTACT+AGG 0.496818 4.4:+78221113 None:intergenic
TTGATTATGGAGAAGGAGAA+GGG 0.504656 4.4:-78221412 MS.gene50329:CDS
TGAAAGAATACAAAGTGTCT+TGG 0.508913 4.4:-78221169 MS.gene50329:CDS
TCAAAGCATGGATCTTCCTC+AGG 0.510641 4.4:-78222621 None:intergenic
TGGTCAACATTATTTGGAAG+AGG 0.517796 4.4:+78221287 None:intergenic
ACATGACCCTTTAATTTCAA+CGG 0.524739 4.4:+78221718 None:intergenic
GGAAGGGAACAGTTATAGTT+CGG 0.528741 4.4:+78221755 None:intergenic
CGGGAAAATAAGAGTCTGGA+AGG 0.536048 4.4:+78221738 None:intergenic
TATGTCGTTGATTATGGAGA+AGG 0.538860 4.4:-78221419 MS.gene50329:CDS
TTGGCTTTGATTTAATGGAA+AGG 0.539588 4.4:-78221325 MS.gene50329:CDS
AAAATATGGTGGTACTAGAT+TGG 0.540379 4.4:-78221024 MS.gene50329:CDS
GAGTCGCTACTTTCACTCTC+TGG 0.542238 4.4:-78220924 MS.gene50329:CDS
TGATCATTCAAATTCTGTTG+AGG 0.546882 4.4:-78222595 MS.gene50329:CDS
CCACATAATCATCTCTAGAC+TGG 0.557773 4.4:+78221564 None:intergenic
CATTAAATCAAAGCCAACAA+TGG 0.566131 4.4:+78221331 None:intergenic
CTCGGTATACACGACAACTT+CGG 0.566711 4.4:+78220944 None:intergenic
GTTGATTATGGAGAAGGAGA+AGG 0.582233 4.4:-78221413 MS.gene50329:CDS
TTATGGAGAAGGAGAAGGGG+TGG 0.588306 4.4:-78221408 MS.gene50329:CDS
TTCGGATGGACTGTTGCAGA+AGG 0.589220 4.4:+78220962 None:intergenic
CATTTCTGGAATAATATAGA+GGG 0.592434 4.4:+78221096 None:intergenic
TACATATGGAATCCATCCAC+CGG 0.600513 4.4:-78221659 MS.gene50329:CDS
GTCTAGAGATGATTATGTGG+TGG 0.603968 4.4:-78221561 MS.gene50329:CDS
ATATGTAGATGTTTGGATGA+CGG 0.606207 4.4:+78221675 None:intergenic
GTATACACGACAACTTCGGA+TGG 0.615119 4.4:+78220948 None:intergenic
CAATACTTGGAAAGAAATCG+AGG 0.619297 4.4:-78221456 MS.gene50329:CDS
TTGGTGAAGTATAATGACAA+AGG 0.639076 4.4:-78221005 MS.gene50329:CDS
GATATTATTGGAAAATATGG+TGG 0.639099 4.4:-78221035 MS.gene50329:CDS
TTACTAGGAATGAAATGCTG+AGG 0.640870 4.4:+78221128 None:intergenic
CCAGTCTAGAGATGATTATG+TGG 0.642654 4.4:-78221564 MS.gene50329:CDS
TGATTATGGAGAAGGAGAAG+GGG 0.649695 4.4:-78221411 MS.gene50329:CDS
ATTTGAATGATCAGTTCCTG+AGG 0.653863 4.4:+78222605 None:intergenic
TCTATATTATTCCAGAAATG+CGG 0.659736 4.4:-78221093 MS.gene50329:CDS
AGAGAGTGAAAGTAGCGACT+CGG 0.677615 4.4:+78220926 None:intergenic
AATACTTGGAAAGAAATCGA+GGG 0.689376 4.4:-78221455 MS.gene50329:CDS
GGGAAAATAAGAGTCTGGAA+GGG 0.700725 4.4:+78221739 None:intergenic
CAGAATGTACTAATGCTCTG+TGG 0.704221 4.4:+78221267 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CGTTTATAATTAAAAAAAAA+AGG - chr4.4:78221421-78221440 MS.gene50329:CDS 10.0%
!! TATTTACTCATATATTAATA+TGG + chr4.4:78221515-78221534 None:intergenic 10.0%
!! AAAACATATATTACTCTATA+TGG - chr4.4:78221920-78221939 MS.gene50329:intron 15.0%
!! ATAATGATACAATTGAAATA+TGG - chr4.4:78222323-78222342 MS.gene50329:intron 15.0%
!! TAATGATACAATTGAAATAT+GGG - chr4.4:78222324-78222343 MS.gene50329:intron 15.0%
!!! ATATAGAGTAATATATGTTT+TGG + chr4.4:78221921-78221940 None:intergenic 15.0%
!!! GATTATTTTCTTTGTAATTT+TGG + chr4.4:78221176-78221195 None:intergenic 15.0%
!! AAAAAAAAAAAAAACTGGTG+TGG - chr4.4:78221618-78221637 MS.gene50329:CDS 20.0%
!! TATATAGAAAGAGATATTCT+TGG + chr4.4:78221250-78221269 None:intergenic 20.0%
!!! CATTTTCTGTAGAATATATA+GGG + chr4.4:78222462-78222481 None:intergenic 20.0%
!!! TAAGAATGGATTACATTTAT+TGG + chr4.4:78221993-78222012 None:intergenic 20.0%
!!! TATATTACTCTATATGGTTT+TGG - chr4.4:78221926-78221945 MS.gene50329:intron 20.0%
! AAAAATATAAGAGACTTCAC+CGG + chr4.4:78220953-78220972 None:intergenic 25.0%
! AAATCAATTGATCGAATTTC+AGG + chr4.4:78221713-78221732 None:intergenic 25.0%
! AAATGTAATCCATTCTTAGT+TGG - chr4.4:78221995-78222014 MS.gene50329:intron 25.0%
! ACAGAAAATGGTGATATTAT+TGG - chr4.4:78222472-78222491 MS.gene50329:intron 25.0%
! CCTATATATTCTACAGAAAA+TGG - chr4.4:78222460-78222479 MS.gene50329:intron 25.0%
! GATATTATTGGAAAATATGG+TGG - chr4.4:78222484-78222503 MS.gene50329:intron 25.0%
! GGTGATATTATTGGAAAATA+TGG - chr4.4:78222481-78222500 MS.gene50329:intron 25.0%
! TCTATATTATTCCAGAAATG+CGG - chr4.4:78222426-78222445 MS.gene50329:intron 25.0%
!! AAATGTGAATTTTCAAAGTG+AGG + chr4.4:78221016-78221035 None:intergenic 25.0%
!! ATTTTCCCGTTGAAATTAAA+GGG - chr4.4:78221795-78221814 MS.gene50329:CDS 25.0%
!! CCATTTTCTGTAGAATATAT+AGG + chr4.4:78222463-78222482 None:intergenic 25.0%
!! TATTTTCCCGTTGAAATTAA+AGG - chr4.4:78221794-78221813 MS.gene50329:CDS 25.0%
!!! CATTTCTGGAATAATATAGA+GGG + chr4.4:78222426-78222445 None:intergenic 25.0%
AAAATATGGTGGTACTAGAT+TGG - chr4.4:78222495-78222514 MS.gene50329:intron 30.0%
AATACTTGGAAAGAAATCGA+GGG - chr4.4:78222064-78222083 MS.gene50329:intron 30.0%
AGAAAAACTCCAAATGTGAT+TGG + chr4.4:78222033-78222052 None:intergenic 30.0%
ATATGTAGATGTTTGGATGA+CGG + chr4.4:78221847-78221866 None:intergenic 30.0%
CATTAAATCAAAGCCAACAA+TGG + chr4.4:78222191-78222210 None:intergenic 30.0%
TCAAATGCGTTTGTAAGTTA+TGG - chr4.4:78220972-78220991 MS.gene50329:CDS 30.0%
TGATCATTCAAATTCTGTTG+AGG - chr4.4:78220924-78220943 MS.gene50329:CDS 30.0%
TGGAGAATTTCTCAGTATAT+GGG - chr4.4:78222288-78222307 MS.gene50329:intron 30.0%
TTGGAGAATTTCTCAGTATA+TGG - chr4.4:78222287-78222306 MS.gene50329:intron 30.0%
TTGGTGAAGTATAATGACAA+AGG - chr4.4:78222514-78222533 MS.gene50329:intron 30.0%
! AATGACAAAGGACAGTTTTT+AGG - chr4.4:78222526-78222545 MS.gene50329:intron 30.0%
! ACATGACCCTTTAATTTCAA+CGG + chr4.4:78221804-78221823 None:intergenic 30.0%
! ATGACAAAGGACAGTTTTTA+GGG - chr4.4:78222527-78222546 MS.gene50329:intron 30.0%
! ATTTCTCAGTATATGGGTTA+AGG - chr4.4:78222294-78222313 MS.gene50329:intron 30.0%
! TGAAAGAATACAAAGTGTCT+TGG - chr4.4:78222350-78222369 MS.gene50329:intron 30.0%
! TTTTACTGTCCAATCACATT+TGG - chr4.4:78222021-78222040 MS.gene50329:intron 30.0%
! TTTTTAATGGGGCTATTCAT+TGG - chr4.4:78222134-78222153 MS.gene50329:intron 30.0%
!! ATTAGTACATTCTGGTTTGT+GGG - chr4.4:78222261-78222280 MS.gene50329:intron 30.0%
!! CATTGTTGGCTTTGATTTAA+TGG - chr4.4:78222189-78222208 MS.gene50329:intron 30.0%
!! GCATTTCTGGAATAATATAG+AGG + chr4.4:78222427-78222446 None:intergenic 30.0%
!! TTGGCTTTGATTTAATGGAA+AGG - chr4.4:78222194-78222213 MS.gene50329:intron 30.0%
AGAGGGTTAAAGTAGTTACT+AGG + chr4.4:78222409-78222428 None:intergenic 35.0%
ATGCATAAAAATCTACGAGC+AGG + chr4.4:78221677-78221696 None:intergenic 35.0%
ATTTGAATGATCAGTTCCTG+AGG + chr4.4:78220917-78220936 None:intergenic 35.0%
CAATACTTGGAAAGAAATCG+AGG - chr4.4:78222063-78222082 MS.gene50329:intron 35.0%
CTCCATAATCAACGACATAA+GGG + chr4.4:78222099-78222118 None:intergenic 35.0%
GGATTCCATATGTAGATGTT+TGG + chr4.4:78221854-78221873 None:intergenic 35.0%
GTAAAACCACCAACTAAGAA+TGG + chr4.4:78222007-78222026 None:intergenic 35.0%
GTCATCCAAACATCTACATA+TGG - chr4.4:78221846-78221865 MS.gene50329:intron 35.0%
TCAAATTCTGTTGAGGTTAC+CGG - chr4.4:78220931-78220950 MS.gene50329:CDS 35.0%
TCTCCATAATCAACGACATA+AGG + chr4.4:78222100-78222119 None:intergenic 35.0%
TCTCGTTTAGAGACAATACT+TGG - chr4.4:78222050-78222069 MS.gene50329:intron 35.0%
TGCATAAAAATCTACGAGCA+GGG + chr4.4:78221676-78221695 None:intergenic 35.0%
TGGTCAACATTATTTGGAAG+AGG + chr4.4:78222235-78222254 None:intergenic 35.0%
TGTAATCCATTCTTAGTTGG+TGG - chr4.4:78221998-78222017 MS.gene50329:intron 35.0%
TTACTAGGAATGAAATGCTG+AGG + chr4.4:78222394-78222413 None:intergenic 35.0%
TTGATTATGGAGAAGGAGAA+GGG - chr4.4:78222107-78222126 MS.gene50329:intron 35.0%
! AAGCATTTGTCTTTTCGATC+CGG + chr4.4:78221882-78221901 None:intergenic 35.0%
! ATCTGGCTTCACAATATTAG+TGG - chr4.4:78221076-78221095 MS.gene50329:CDS 35.0%
! CATGACCCTTTAATTTCAAC+GGG + chr4.4:78221803-78221822 None:intergenic 35.0%
! TATGTCGTTGATTATGGAGA+AGG - chr4.4:78222100-78222119 MS.gene50329:intron 35.0%
! TCTGGCTTCACAATATTAGT+GGG - chr4.4:78221077-78221096 MS.gene50329:CDS 35.0%
! TTCCCTTATGTCGTTGATTA+TGG - chr4.4:78222094-78222113 MS.gene50329:intron 35.0%
!! CATTAGTACATTCTGGTTTG+TGG - chr4.4:78222260-78222279 MS.gene50329:intron 35.0%
CAGAATGTACTAATGCTCTG+TGG + chr4.4:78222255-78222274 None:intergenic 40.0%
CCACATAATCATCTCTAGAC+TGG + chr4.4:78221958-78221977 None:intergenic 40.0%
CCAGTCTAGAGATGATTATG+TGG - chr4.4:78221955-78221974 MS.gene50329:intron 40.0%
CTGGCTTCACAATATTAGTG+GGG - chr4.4:78221078-78221097 MS.gene50329:CDS 40.0%
GCATAAAAATCTACGAGCAG+GGG + chr4.4:78221675-78221694 None:intergenic 40.0%
GCTCTGTGGTCAACATTATT+TGG + chr4.4:78222241-78222260 None:intergenic 40.0%
GGAAGGGAACAGTTATAGTT+CGG + chr4.4:78221767-78221786 None:intergenic 40.0%
GGGAAAATAAGAGTCTGGAA+GGG + chr4.4:78221783-78221802 None:intergenic 40.0%
GTCTAGAGATGATTATGTGG+TGG - chr4.4:78221958-78221977 MS.gene50329:intron 40.0%
GTTGATTATGGAGAAGGAGA+AGG - chr4.4:78222106-78222125 MS.gene50329:intron 40.0%
TAATGGGGCTATTCATTGGT+TGG - chr4.4:78222138-78222157 MS.gene50329:intron 40.0%
TACATATGGAATCCATCCAC+CGG - chr4.4:78221860-78221879 MS.gene50329:intron 40.0%
TCAACGGGAAAATAAGAGTC+TGG + chr4.4:78221788-78221807 None:intergenic 40.0%
TGATTATGGAGAAGGAGAAG+GGG - chr4.4:78222108-78222127 MS.gene50329:intron 40.0%
TGTGTGTTGCAAGGTTAAGT+TGG + chr4.4:78221039-78221058 None:intergenic 40.0%
! AGAAGGGGTGGTTTTTAATG+GGG - chr4.4:78222123-78222142 MS.gene50329:intron 40.0%
! CACAGAGCATTAGTACATTC+TGG - chr4.4:78222253-78222272 MS.gene50329:intron 40.0%
! GAGAAGGGGTGGTTTTTAAT+GGG - chr4.4:78222122-78222141 MS.gene50329:intron 40.0%
!! ATAGCTTTGGATGCCATTGT+TGG - chr4.4:78222175-78222194 MS.gene50329:intron 40.0%
!! CATTCTGGTTTGTGGGTATT+TGG - chr4.4:78222268-78222287 MS.gene50329:intron 40.0%
!!! CGTGTTGCGCTCTTTTTTTT+TGG - chr4.4:78221325-78221344 MS.gene50329:CDS 40.0%
AGAGAGTGAAAGTAGCGACT+CGG + chr4.4:78222596-78222615 None:intergenic 45.0%
CGGGAAAATAAGAGTCTGGA+AGG + chr4.4:78221784-78221803 None:intergenic 45.0%
CTCGGTATACACGACAACTT+CGG + chr4.4:78222578-78222597 None:intergenic 45.0%
GATGAGTCAAATGCTCTAGC+AGG - chr4.4:78221280-78221299 MS.gene50329:CDS 45.0%
GTATACACGACAACTTCGGA+TGG + chr4.4:78222574-78222593 None:intergenic 45.0%
GTGAAAGTAGTCCGCATTTC+TGG + chr4.4:78222440-78222459 None:intergenic 45.0%
TAAAAATCTACGAGCAGGGG+CGG + chr4.4:78221672-78221691 None:intergenic 45.0%
TTCGCGTCGTGATATAGCTT+TGG - chr4.4:78222162-78222181 MS.gene50329:intron 45.0%
! CAGTTATAGTTCGGTGAAGC+AGG + chr4.4:78221758-78221777 None:intergenic 45.0%
! CATTTGTCTTTTCGATCCGG+TGG + chr4.4:78221879-78221898 None:intergenic 45.0%
! GGAGAAGGGGTGGTTTTTAA+TGG - chr4.4:78222121-78222140 MS.gene50329:intron 45.0%
!! AAAAAAAAAAAAAAAAAAAC+TGG - chr4.4:78221613-78221632 MS.gene50329:CDS 5.0%
AGCAGGATACCCGTGCATAT+GGG - chr4.4:78221297-78221316 MS.gene50329:CDS 50.0%
CAACACACACTCGTAGACAG+GGG - chr4.4:78221050-78221069 MS.gene50329:CDS 50.0%
GCAACACACACTCGTAGACA+GGG - chr4.4:78221049-78221068 MS.gene50329:CDS 50.0%
GTCTACGAGTGTGTGTTGCA+AGG + chr4.4:78221048-78221067 None:intergenic 50.0%
TAGCAGGATACCCGTGCATA+TGG - chr4.4:78221296-78221315 MS.gene50329:CDS 50.0%
TGCAACACACACTCGTAGAC+AGG - chr4.4:78221048-78221067 MS.gene50329:CDS 50.0%
TTATGGAGAAGGAGAAGGGG+TGG - chr4.4:78222111-78222130 MS.gene50329:intron 50.0%
! GAGTCGCTACTTTCACTCTC+TGG - chr4.4:78222595-78222614 MS.gene50329:CDS 50.0%
! TGTCTTTTCGATCCGGTGGA+TGG + chr4.4:78221875-78221894 None:intergenic 50.0%
!! TTCGGATGGACTGTTGCAGA+AGG + chr4.4:78222560-78222579 None:intergenic 50.0%
ACACGCTCACCCCATATGCA+CGG + chr4.4:78221310-78221329 None:intergenic 55.0%
GCAGGATACCCGTGCATATG+GGG - chr4.4:78221298-78221317 MS.gene50329:CDS 55.0%
TTGCCACACCATGCCCTGTT+GGG - chr4.4:78221644-78221663 MS.gene50329:CDS 55.0%
CACACACTCGTAGACAGGGG+CGG - chr4.4:78221053-78221072 MS.gene50329:CDS 60.0%
CACGCTCACCCCATATGCAC+GGG + chr4.4:78221309-78221328 None:intergenic 60.0%
CTTGCCACACCATGCCCTGT+TGG - chr4.4:78221643-78221662 MS.gene50329:CDS 60.0%
GATCCCAACAGGGCATGGTG+TGG + chr4.4:78221650-78221669 None:intergenic 60.0%
AGCAGGGGCGGATCCCAACA+GGG + chr4.4:78221660-78221679 None:intergenic 65.0%
CTCGTAGACAGGGGCGGATC+TGG - chr4.4:78221059-78221078 MS.gene50329:CDS 65.0%
GAGCAGGGGCGGATCCCAAC+AGG + chr4.4:78221661-78221680 None:intergenic 70.0%
GGGCGGATCCCAACAGGGCA+TGG + chr4.4:78221655-78221674 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 78220905 78222636 78220905 ID=MS.gene50329
chr4.4 mRNA 78220905 78222636 78220905 ID=MS.gene50329.t1;Parent=MS.gene50329
chr4.4 exon 78222558 78222636 78222558 ID=MS.gene50329.t1.exon1;Parent=MS.gene50329.t1
chr4.4 CDS 78222558 78222636 78222558 ID=cds.MS.gene50329.t1;Parent=MS.gene50329.t1
chr4.4 exon 78220905 78221839 78220905 ID=MS.gene50329.t1.exon2;Parent=MS.gene50329.t1
chr4.4 CDS 78220905 78221839 78220905 ID=cds.MS.gene50329.t1;Parent=MS.gene50329.t1
Gene Sequence

>MS.gene50329

ATGGATCTTCCTCAGGAACTGATCATTCAAATTCTGTTGAGGTTACCGGTGAAGTCTCTTATATTTTTCAAATGCGTTTGTAAGTTATGGTTTTCTATTATCTCTGATCCTCACTTTGAAAATTCACATTTCCAACTTAACCTTGCAACACACACTCGTAGACAGGGGCGGATCTGGCTTCACAATATTAGTGGGGCAAAAAAATTTTGTCAAACAATGAAATAATATTAAATTTAATAACAAGTTATATTAAAAATAGTTGTAGAAACCAAAATTACAAAGAAAATAATCTATTTTTCATTTGTTGAAAATGAACTAAAATTATATCATTTACTAATTGTTCCAAGAATATCTCTTTCTATATAGATTACAAGAGATGAGTCAAATGCTCTAGCAGGATACCCGTGCATATGGGGTGAGCGTGTTGCGCTCTTTTTTTTTGGAAGAAATTCGCAATTGCGCAGAAATTATAAAATAAAATATCGTTGTATTGATATATGAGTAAAAATATAGACACGTTTATAATTAAAAAAAAAAGGATTCTCTTATTAATATGAGTAAAAATATAGACACATATATAAATTAAAATATATATATATATATAACACCATATTAATATATGAGTAAATATATAGACTCATGTATAAACATAGAAAATATTAGATGTTTGCATTAATATATGAGTAAAATTATACAATGAATTGATAAAAAAAAAAAAAAAAAAAAACTGGTGTGGCACTTGCCACACCATGCCCTGTTGGGATCCGCCCCTGCTCGTAGATTTTTATGCATTTCAGCTCTATCTCCTGAAATTCGATCAATTGATTTTGATGCATTTCTTAACGATGATCCTGCTTCACCGAACTATAACTGTTCCCTTCCAGACTCTTATTTTCCCGTTGAAATTAAAGGGTCATGTAGATGTTTTATATTCTTGTACCGTCATCCAAACATCTACATATGGAATCCATCCACCGGATCGAAAAGACAAATGCTTATATCTCATTTTAATTCCAAAACATATATTACTCTATATGGTTTTGGTTATGACCAGTCTAGAGATGATTATGTGGTGGTTTTACTGTCCAATAAATGTAATCCATTCTTAGTTGGTGGTTTTACTGTCCAATCACATTTGGAGTTTTTCTCGTTTAGAGACAATACTTGGAAAGAAATCGAGGGTGCTCACTTCCCTTATGTCGTTGATTATGGAGAAGGAGAAGGGGTGGTTTTTAATGGGGCTATTCATTGGTTGGCTTCGCGTCGTGATATAGCTTTGGATGCCATTGTTGGCTTTGATTTAATGGAAAGGAAATTGTTTGATATGCCTCTTCCAAATAATGTTGACCACAGAGCATTAGTACATTCTGGTTTGTGGGTATTTGGAGAATTTCTCAGTATATGGGTTAAGGATACTGATAATGATACAATTGAAATATGGGTGATGAAAGAATACAAAGTGTCTTGGACTAAGAATCTTGTTCTTCCTCAGCATTTCATTCCTAGTAACTACTTTAACCCTCTATATTATTCCAGAAATGCGGACTACTTTCACCCTATATATTCTACAGAAAATGGTGATATTATTGGAAAATATGGTGGTACTAGATTGGTGAAGTATAATGACAAAGGACAGTTTTTAGGGCATCGCTCCTTCTGCAACAGTCCATCCGAAGTTGTCGTGTATACCGAGTCGCTACTTTCACTCTCTGGTGACAATGAGCATGTTTAA
Protein sequence

>MS.gene50329.t1

MDLPQELIIQILLRLPVKSLIFFKCVSLSPEIRSIDFDAFLNDDPASPNYNCSLPDSYFPVEIKGSCRCFIFLYRHPNIYIWNPSTGSKRQMLISHFNSKTYITLYGFGYDQSRDDYVVVLLSNKCNPFLVGGFTVQSHLEFFSFRDNTWKEIEGAHFPYVVDYGEGEGVVFNGAIHWLASRRDIALDAIVGFDLMERKLFDMPLPNNVDHRALVHSGLWVFGEFLSIWVKDTDNDTIEIWVMKEYKVSWTKNLVLPQHFIPSNYFNPLYYSRNADYFHPIYSTENGDIIGKYGGTRLVKYNDKGQFLGHRSFCNSPSEVVVYTESLLSLSGDNEHV