AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene038756


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene038756.t1 MTR_3g103020 62.069 203 72 3 7 208 180 378 3.69e-80 244
MS.gene038756.t1 MTR_4g117720 62.201 209 66 7 6 208 177 378 2.27e-78 239
MS.gene038756.t1 MTR_6g060650 61.500 200 69 4 8 207 181 372 2.89e-76 234
MS.gene038756.t1 MTR_4g109270 58.654 208 77 4 1 208 166 364 7.27e-73 225
MS.gene038756.t1 MTR_4g109280 61.500 200 67 6 11 208 168 359 8.63e-73 224
MS.gene038756.t1 MTR_8g040935 55.924 211 87 3 1 208 1 208 6.91e-68 207
MS.gene038756.t1 MTR_3g034180 58.824 204 74 5 8 207 291 488 3.76e-67 214
MS.gene038756.t1 MTR_4g117850 58.163 196 77 4 8 201 181 373 5.70e-66 207
MS.gene038756.t1 MTR_4g122850 55.288 208 85 7 4 208 162 364 8.34e-66 207
MS.gene038756.t1 MTR_4g117770 58.621 203 76 4 9 208 170 367 1.13e-62 199
MS.gene038756.t1 MTR_4g117810 58.621 203 76 4 9 208 170 367 1.13e-62 199
MS.gene038756.t1 MTR_4g023130 55.340 206 83 5 2 207 116 312 1.68e-62 197
MS.gene038756.t1 MTR_3g034130 56.345 197 76 5 3 194 171 362 1.73e-62 198
MS.gene038756.t1 MTR_4g116330 55.556 198 80 4 13 208 189 380 2.98e-62 198
MS.gene038756.t1 MTR_4g116320 55.000 200 80 5 8 205 64 255 4.29e-62 194
MS.gene038756.t1 MTR_3g034160 56.667 210 80 6 3 207 171 374 8.38e-62 197
MS.gene038756.t1 MTR_4g094365 54.950 202 80 6 10 208 171 364 9.78e-62 196
MS.gene038756.t1 MTR_4g117780 57.143 203 79 4 9 208 170 367 2.79e-61 195
MS.gene038756.t1 MTR_4g023080 54.369 206 85 5 2 207 157 353 3.48e-61 194
MS.gene038756.t1 MTR_4g021785 54.589 207 86 5 2 208 157 355 1.18e-59 191
MS.gene038756.t1 MTR_4g023070 54.286 210 74 6 11 207 169 369 1.28e-59 191
MS.gene038756.t1 MTR_4g023090 50.000 216 85 6 6 207 177 383 5.80e-58 187
MS.gene038756.t1 MTR_4g118380 55.738 183 64 5 8 181 183 357 5.94e-57 184
MS.gene038756.t1 MTR_4g118400 55.385 195 67 6 8 202 120 294 1.76e-56 181
MS.gene038756.t1 MTR_8g433030 53.465 202 83 8 4 202 143 336 8.77e-56 180
MS.gene038756.t1 MTR_4g023120 51.659 211 80 6 11 208 169 370 1.25e-55 181
MS.gene038756.t1 MTR_8g032390 53.465 202 83 8 4 202 176 369 2.82e-55 180
MS.gene038756.t1 MTR_6g463430 48.585 212 95 6 1 208 1 202 2.05e-54 172
MS.gene038756.t1 MTR_4g023380 48.848 217 80 6 13 207 169 376 2.79e-54 177
MS.gene038756.t1 MTR_8g433040 50.481 208 85 5 2 208 174 364 9.75e-53 173
MS.gene038756.t1 MTR_8g032380 50.481 208 85 5 2 208 174 364 9.75e-53 173
MS.gene038756.t1 MTR_5g097210 49.749 199 90 6 8 204 169 359 9.89e-52 171
MS.gene038756.t1 MTR_1g055175 48.259 201 91 8 13 208 9 201 1.40e-51 165
MS.gene038756.t1 MTR_4g023190 52.683 205 81 9 7 206 164 357 2.26e-51 169
MS.gene038756.t1 MTR_5g070080 48.095 210 101 6 1 208 183 386 2.00e-48 162
MS.gene038756.t1 MTR_4g117530 46.701 197 66 6 12 208 96 253 2.26e-48 159
MS.gene038756.t1 MTR_4g023200 49.268 205 89 9 7 206 164 358 5.42e-47 158
MS.gene038756.t1 MTR_1g055195 46.377 207 89 7 13 208 283 478 5.45e-47 161
MS.gene038756.t1 MTR_1g055135 47.343 207 97 6 8 208 175 375 2.30e-46 157
MS.gene038756.t1 MTR_4g117870 45.320 203 69 4 6 208 107 267 2.38e-46 154
MS.gene038756.t1 MTR_4g023150 50.244 205 87 9 7 206 164 358 3.95e-46 156
MS.gene038756.t1 MTR_1g055165 51.852 162 71 5 49 208 162 318 3.96e-46 155
MS.gene038756.t1 MTR_4g117750 44.878 205 98 6 11 207 161 358 6.95e-44 153
MS.gene038756.t1 MTR_4g117750 60.000 110 42 1 98 207 373 480 1.88e-35 130
MS.gene038756.t1 MTR_7g079400 45.673 208 96 9 10 208 138 337 1.74e-43 148
MS.gene038756.t1 MTR_5g070130 43.627 204 105 5 11 208 176 375 2.02e-43 149
MS.gene038756.t1 MTR_4g118370 43.590 195 85 5 11 204 86 256 1.24e-42 144
MS.gene038756.t1 MTR_8g102520 41.346 208 111 6 5 208 122 322 2.09e-42 145
MS.gene038756.t1 MTR_4g023440 44.724 199 64 5 10 208 180 332 2.04e-39 138
MS.gene038756.t1 MTR_4g121580 45.596 193 69 6 8 198 181 339 1.56e-38 137
MS.gene038756.t1 MTR_1g055250 42.000 200 101 8 8 201 73 263 1.96e-38 135
MS.gene038756.t1 MTR_4g118360 56.154 130 49 5 83 208 1 126 5.20e-38 128
MS.gene038756.t1 MTR_4g116330 43.939 198 64 4 13 208 189 341 6.69e-38 134
MS.gene038756.t1 MTR_5g070088 43.434 198 96 5 11 202 192 379 2.19e-37 134
MS.gene038756.t1 MTR_4g117430 63.303 109 28 4 1 109 58 154 2.58e-35 122
MS.gene038756.t1 MTR_7g079370 43.077 195 97 10 13 202 165 350 2.00e-34 125
MS.gene038756.t1 MTR_5g023000 61.702 94 36 0 115 208 6 99 4.15e-34 117
MS.gene038756.t1 MTR_4g122860 40.513 195 81 5 18 208 157 320 5.96e-33 121
MS.gene038756.t1 MTR_8g098830 43.931 173 86 7 9 178 31 195 2.15e-32 117
MS.gene038756.t1 MTR_4g114700 39.791 191 60 5 11 198 79 217 2.80e-29 109
MS.gene038756.t1 MTR_2g048580 37.264 212 109 12 3 208 149 342 1.11e-28 110
MS.gene038756.t1 MTR_7g079420 38.830 188 97 9 30 208 966 1144 5.24e-27 108
MS.gene038756.t1 MTR_7g079450 41.549 142 76 4 68 208 2 137 2.98e-24 93.6
MS.gene038756.t1 MTR_8g013010 55.263 114 39 6 1 113 212 314 6.21e-24 97.1
MS.gene038756.t1 MTR_8g013005 66.102 59 20 0 150 208 1 59 5.84e-20 80.1
MS.gene038756.t1 MTR_7g117720 56.977 86 33 2 121 202 186 271 1.90e-19 84.0
MS.gene038756.t1 MTR_4g023430 38.017 121 57 3 94 208 114 222 1.22e-16 75.5
MS.gene038756.t1 MTR_6g034720 46.739 92 41 3 8 97 99 184 1.14e-14 70.1
MS.gene038756.t1 MTR_4g109290 50.000 70 31 1 139 208 123 188 2.89e-11 60.5
MS.gene038756.t1 MTR_2g064960 64.286 42 15 0 167 208 18 59 7.88e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene038756.t1 AT3G06240 30.256 195 108 9 16 200 243 419 4.22e-12 64.7

Find 50 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAGAATTTCTTAGTTTA+TGG 0.111007 8.3:+14229718 MS.gene038756:CDS
GTCATGATTCACGTTTATTA+AGG 0.188886 8.3:+14229595 MS.gene038756:CDS
TATTGTGGTTTGTGGGTATT+TGG 0.253702 8.3:+14229699 MS.gene038756:CDS
ATATTGATACAATTGAAATA+TGG 0.279816 8.3:+14229754 MS.gene038756:CDS
TGGAGAATTTCTTAGTTTAT+GGG 0.300088 8.3:+14229719 MS.gene038756:CDS
TATTGTTGTCTTTGATTTAA+CGG 0.303490 8.3:+14229623 MS.gene038756:CDS
TACTAATATTTCATCGCAAT+TGG 0.320204 8.3:+14229434 MS.gene038756:CDS
TATTGATACAATTGAAATAT+GGG 0.363603 8.3:+14229755 MS.gene038756:CDS
ATTGAAACAGATGGTGATAC+TGG 0.365088 8.3:+14229891 MS.gene038756:CDS
TCATGATTCACGTTTATTAA+GGG 0.367232 8.3:+14229596 MS.gene038756:CDS
CCTCAATGGGATTGCTATAA+CGG 0.368433 8.3:-14229515 None:intergenic
TGCGATGAAATATTAGTAAC+CGG 0.431931 8.3:-14229429 None:intergenic
AATACAAAATGCCTTCATCT+TGG 0.434813 8.3:+14229787 MS.gene038756:CDS
TATTTAATGAGGCTATTCAT+TGG 0.437891 8.3:+14229562 MS.gene038756:CDS
GGTTTCAATGCTCATTGATC+CGG 0.441203 8.3:+14229410 None:intergenic
CTTAGGCTGCTCATCCTCAA+TGG 0.445603 8.3:-14229529 None:intergenic
TTAAATAGCAACCCCGGCTT+AGG 0.449345 8.3:-14229546 None:intergenic
AACTTGACTATTGTGGTTTG+TGG 0.450962 8.3:+14229691 MS.gene038756:CDS
AACCACAATAGTCAAGTTGA+TGG 0.467522 8.3:-14229686 None:intergenic
ATTTCTTAGTTTATGGGCTA+AGG 0.469399 8.3:+14229725 MS.gene038756:CDS
TGTCTTTGATTTAACGGAAA+GGG 0.470268 8.3:+14229629 MS.gene038756:CDS
TAATGAGGCTATTCATTGGT+TGG 0.476284 8.3:+14229566 MS.gene038756:CDS
AGTTGATGGTCAAACTCATC+TGG 0.476777 8.3:-14229672 None:intergenic
TTAGGCTGCTCATCCTCAAT+GGG 0.484131 8.3:-14229528 None:intergenic
GAGGATGAGCAGCCTAAGCC+GGG 0.489365 8.3:+14229534 MS.gene038756:CDS
TGAGGATGAGCAGCCTAAGC+CGG 0.491054 8.3:+14229533 MS.gene038756:CDS
ACTTGACTATTGTGGTTTGT+GGG 0.504506 8.3:+14229692 MS.gene038756:CDS
CTGATTTCACTTCCTTGTGA+CGG 0.505657 8.3:+14230008 MS.gene038756:CDS
CCGTTATAGCAATCCCATTG+AGG 0.526015 8.3:+14229515 MS.gene038756:CDS
GCCGGGGTTGCTATTTAATG+AGG 0.531997 8.3:+14229551 MS.gene038756:CDS
GACCATCAACTTGACTATTG+TGG 0.546126 8.3:+14229684 MS.gene038756:CDS
TTGTCTTTGATTTAACGGAA+AGG 0.568763 8.3:+14229628 MS.gene038756:CDS
TGAGTCTTAGTCCAAGATGA+AGG 0.569111 8.3:-14229798 None:intergenic
GTGGAAAGAAATTGAGATCG+AGG 0.598854 8.3:+14229482 MS.gene038756:CDS
TGGAAAGAAATTGAGATCGA+GGG 0.601685 8.3:+14229483 MS.gene038756:CDS
GCCTCATTAAATAGCAACCC+CGG 0.605181 8.3:-14229552 None:intergenic
AACAGATGGTGATACTGGAT+TGG 0.615393 8.3:+14229896 MS.gene038756:CDS
TCCATGTGAATCGTTGCGGA+AGG 0.617460 8.3:-14229958 None:intergenic
TAAGCTTGTTCACCGTCACA+AGG 0.619980 8.3:-14230020 None:intergenic
AATGGAGAAAAGTAATCTGT+CGG 0.624209 8.3:-14229843 None:intergenic
GGTGATATTATTGAAACAGA+TGG 0.625686 8.3:+14229882 MS.gene038756:CDS
CTCGGTATATATAGCCACAT+GGG 0.627450 8.3:-14229982 None:intergenic
GGGATCCATGTGAATCGTTG+CGG 0.632127 8.3:-14229962 None:intergenic
TCCTTCCGCAACGATTCACA+TGG 0.639682 8.3:+14229957 MS.gene038756:CDS
ACTCGGTATATATAGCCACA+TGG 0.649360 8.3:-14229983 None:intergenic
AGGAAGTGAAATCAGAGACT+CGG 0.652262 8.3:-14230000 None:intergenic
TCTCGTTGAGAGATAATACG+TGG 0.669859 8.3:+14229463 MS.gene038756:CDS
CGATTCACATGGATCCCATG+TGG 0.708556 8.3:+14229968 MS.gene038756:CDS
AGGATGAGCAGCCTAAGCCG+GGG 0.728363 8.3:+14229535 MS.gene038756:CDS
TTGGTGAAGTCTGATGACAA+AGG 0.797487 8.3:+14229915 MS.gene038756:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTGATACAATTGAAATA+TGG + chr8.3:14229754-14229773 MS.gene038756:CDS 15.0%
!! TATTGATACAATTGAAATAT+GGG + chr8.3:14229755-14229774 MS.gene038756:CDS 15.0%
!!! TATTGTTGTCTTTGATTTAA+CGG + chr8.3:14229623-14229642 MS.gene038756:CDS 20.0%
! TACTAATATTTCATCGCAAT+TGG + chr8.3:14229434-14229453 MS.gene038756:CDS 25.0%
! TATTTAATGAGGCTATTCAT+TGG + chr8.3:14229562-14229581 MS.gene038756:CDS 25.0%
! TCATGATTCACGTTTATTAA+GGG + chr8.3:14229596-14229615 MS.gene038756:CDS 25.0%
! TGGAGAATTTCTTAGTTTAT+GGG + chr8.3:14229719-14229738 MS.gene038756:CDS 25.0%
! TTGGAGAATTTCTTAGTTTA+TGG + chr8.3:14229718-14229737 MS.gene038756:CDS 25.0%
AATACAAAATGCCTTCATCT+TGG + chr8.3:14229787-14229806 MS.gene038756:CDS 30.0%
AATGGAGAAAAGTAATCTGT+CGG - chr8.3:14229846-14229865 None:intergenic 30.0%
GGTGATATTATTGAAACAGA+TGG + chr8.3:14229882-14229901 MS.gene038756:CDS 30.0%
GTCATGATTCACGTTTATTA+AGG + chr8.3:14229595-14229614 MS.gene038756:CDS 30.0%
TGCGATGAAATATTAGTAAC+CGG - chr8.3:14229432-14229451 None:intergenic 30.0%
! ATTTCTTAGTTTATGGGCTA+AGG + chr8.3:14229725-14229744 MS.gene038756:CDS 30.0%
! TGTCTTTGATTTAACGGAAA+GGG + chr8.3:14229629-14229648 MS.gene038756:CDS 30.0%
! TTGTCTTTGATTTAACGGAA+AGG + chr8.3:14229628-14229647 MS.gene038756:CDS 30.0%
AACTTGACTATTGTGGTTTG+TGG + chr8.3:14229691-14229710 MS.gene038756:CDS 35.0%
ATTGAAACAGATGGTGATAC+TGG + chr8.3:14229891-14229910 MS.gene038756:CDS 35.0%
CCATTGTGTTGTACAAAAAG+TGG + chr8.3:14229861-14229880 MS.gene038756:CDS 35.0%
TAATGAGGCTATTCATTGGT+TGG + chr8.3:14229566-14229585 MS.gene038756:CDS 35.0%
TGGAAAGAAATTGAGATCGA+GGG + chr8.3:14229483-14229502 MS.gene038756:CDS 35.0%
! ACTTGACTATTGTGGTTTGT+GGG + chr8.3:14229692-14229711 MS.gene038756:CDS 35.0%
! GGAAAGGGAACTTTTTGATA+TGG + chr8.3:14229644-14229663 MS.gene038756:CDS 35.0%
! TATTGTGGTTTGTGGGTATT+TGG + chr8.3:14229699-14229718 MS.gene038756:CDS 35.0%
!! AACCACAATAGTCAAGTTGA+TGG - chr8.3:14229689-14229708 None:intergenic 35.0%
!!! CCACTTTTTGTACAACACAA+TGG - chr8.3:14229864-14229883 None:intergenic 35.0%
AACAGATGGTGATACTGGAT+TGG + chr8.3:14229896-14229915 MS.gene038756:CDS 40.0%
ACTCGGTATATATAGCCACA+TGG - chr8.3:14229986-14230005 None:intergenic 40.0%
AGGAAGTGAAATCAGAGACT+CGG - chr8.3:14230003-14230022 None:intergenic 40.0%
CCTCAATGGGATTGCTATAA+CGG - chr8.3:14229518-14229537 None:intergenic 40.0%
CTCGGTATATATAGCCACAT+GGG - chr8.3:14229985-14230004 None:intergenic 40.0%
CTGATTTCACTTCCTTGTGA+CGG + chr8.3:14230008-14230027 MS.gene038756:CDS 40.0%
GACCATCAACTTGACTATTG+TGG + chr8.3:14229684-14229703 MS.gene038756:CDS 40.0%
GTGGAAAGAAATTGAGATCG+AGG + chr8.3:14229482-14229501 MS.gene038756:CDS 40.0%
TCTCGTTGAGAGATAATACG+TGG + chr8.3:14229463-14229482 MS.gene038756:CDS 40.0%
TGAGTCTTAGTCCAAGATGA+AGG - chr8.3:14229801-14229820 None:intergenic 40.0%
TTGGTGAAGTCTGATGACAA+AGG + chr8.3:14229915-14229934 MS.gene038756:CDS 40.0%
!! AGTTGATGGTCAAACTCATC+TGG - chr8.3:14229675-14229694 None:intergenic 40.0%
CCGTTATAGCAATCCCATTG+AGG + chr8.3:14229515-14229534 MS.gene038756:CDS 45.0%
GCCTCATTAAATAGCAACCC+CGG - chr8.3:14229555-14229574 None:intergenic 45.0%
TTAAATAGCAACCCCGGCTT+AGG - chr8.3:14229549-14229568 None:intergenic 45.0%
TTAGGCTGCTCATCCTCAAT+GGG - chr8.3:14229531-14229550 None:intergenic 45.0%
! TAAGCTTGTTCACCGTCACA+AGG - chr8.3:14230023-14230042 None:intergenic 45.0%
CGATTCACATGGATCCCATG+TGG + chr8.3:14229968-14229987 MS.gene038756:CDS 50.0%
CTTAGGCTGCTCATCCTCAA+TGG - chr8.3:14229532-14229551 None:intergenic 50.0%
GGGATCCATGTGAATCGTTG+CGG - chr8.3:14229965-14229984 None:intergenic 50.0%
TCCATGTGAATCGTTGCGGA+AGG - chr8.3:14229961-14229980 None:intergenic 50.0%
TCCTTCCGCAACGATTCACA+TGG + chr8.3:14229957-14229976 MS.gene038756:CDS 50.0%
!! GCCGGGGTTGCTATTTAATG+AGG + chr8.3:14229551-14229570 MS.gene038756:CDS 50.0%
TGAGGATGAGCAGCCTAAGC+CGG + chr8.3:14229533-14229552 MS.gene038756:CDS 55.0%
AGGATGAGCAGCCTAAGCCG+GGG + chr8.3:14229535-14229554 MS.gene038756:CDS 60.0%
GAGGATGAGCAGCCTAAGCC+GGG + chr8.3:14229534-14229553 MS.gene038756:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 14229417 14230043 14229417 ID=MS.gene038756
chr8.3 mRNA 14229417 14230043 14229417 ID=MS.gene038756.t1;Parent=MS.gene038756
chr8.3 exon 14229417 14230043 14229417 ID=MS.gene038756.t1.exon1;Parent=MS.gene038756.t1
chr8.3 CDS 14229417 14230043 14229417 ID=cds.MS.gene038756.t1;Parent=MS.gene038756.t1
Gene Sequence

>MS.gene038756

ATGCTCATTGATCCGGTTACTAATATTTCATCGCAATTGGAGTTTTTCTCGTTGAGAGATAATACGTGGAAAGAAATTGAGATCGAGGGTAGTCATTTCCGTTATAGCAATCCCATTGAGGATGAGCAGCCTAAGCCGGGGTTGCTATTTAATGAGGCTATTCATTGGTTGGCTTATCGTCATGATTCACGTTTATTAAGGGATGTTATTGTTGTCTTTGATTTAACGGAAAGGGAACTTTTTGATATGGATTTGCCAGATGAGTTTGACCATCAACTTGACTATTGTGGTTTGTGGGTATTTGGAGAATTTCTTAGTTTATGGGCTAAGGATTGTTATATTGATACAATTGAAATATGGGTGATGAAAGAATACAAAATGCCTTCATCTTGGACTAAGACTCATGTTCTGTCTTTTGATAGTATGCCGACAGATTACTTTTCTCCATTGTGTTGTACAAAAAGTGGTGATATTATTGAAACAGATGGTGATACTGGATTGGTGAAGTCTGATGACAAAGGACAACTGCTTGAGCATCGCTCCTTCCGCAACGATTCACATGGATCCCATGTGGCTATATATACCGAGTCTCTGATTTCACTTCCTTGTGACGGTGAACAAGCTTAA

Protein sequence

>MS.gene038756.t1

MLIDPVTNISSQLEFFSLRDNTWKEIEIEGSHFRYSNPIEDEQPKPGLLFNEAIHWLAYRHDSRLLRDVIVVFDLTERELFDMDLPDEFDHQLDYCGLWVFGEFLSLWAKDCYIDTIEIWVMKEYKMPSSWTKTHVLSFDSMPTDYFSPLCCTKSGDIIETDGDTGLVKSDDKGQLLEHRSFRNDSHGSHVAIYTESLISLPCDGEQA