AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58316


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58316.t1 MTR_8g433040 73.228 127 30 2 2 124 238 364 5.29e-58 183
MS.gene58316.t1 MTR_8g032380 73.228 127 30 2 2 124 238 364 5.29e-58 183
MS.gene58316.t1 MTR_8g433030 69.919 123 32 2 2 119 215 337 1.05e-51 166
MS.gene58316.t1 MTR_8g032390 69.919 123 32 2 2 119 248 370 2.89e-51 166
MS.gene58316.t1 MTR_3g103020 56.923 130 49 2 2 124 249 378 6.23e-40 137
MS.gene58316.t1 MTR_4g116330 56.061 132 49 2 2 124 249 380 9.99e-40 136
MS.gene58316.t1 MTR_4g023130 57.480 127 48 2 2 123 187 312 1.23e-39 134
MS.gene58316.t1 MTR_8g040935 54.545 132 51 2 2 124 77 208 2.89e-39 130
MS.gene58316.t1 MTR_4g021785 55.469 128 51 2 2 124 229 355 5.42e-39 134
MS.gene58316.t1 MTR_4g109270 55.385 130 51 2 2 124 235 364 6.38e-39 134
MS.gene58316.t1 MTR_4g094365 57.252 131 48 3 2 124 234 364 2.64e-38 132
MS.gene58316.t1 MTR_4g117870 55.469 128 50 3 3 124 141 267 4.24e-38 129
MS.gene58316.t1 MTR_6g060650 54.264 129 50 3 2 123 246 372 4.82e-38 132
MS.gene58316.t1 MTR_6g463430 53.968 126 55 2 2 124 77 202 9.04e-38 127
MS.gene58316.t1 MTR_4g023080 55.118 127 51 2 2 123 228 353 9.98e-38 130
MS.gene58316.t1 MTR_4g023380 50.658 152 46 5 2 124 228 379 3.34e-37 130
MS.gene58316.t1 MTR_4g109280 53.788 132 49 4 2 124 231 359 2.10e-35 124
MS.gene58316.t1 MTR_4g122850 57.895 133 43 6 2 124 235 364 3.32e-35 124
MS.gene58316.t1 MTR_4g023070 48.921 139 53 2 3 123 231 369 7.99e-35 123
MS.gene58316.t1 MTR_4g117720 53.676 136 48 5 2 124 245 378 8.67e-35 123
MS.gene58316.t1 MTR_4g023090 48.611 144 53 4 2 124 243 386 2.88e-34 122
MS.gene58316.t1 MTR_3g034180 54.615 130 49 4 2 124 363 489 3.28e-34 123
MS.gene58316.t1 MTR_4g116320 54.688 128 47 3 2 120 129 254 3.47e-34 119
MS.gene58316.t1 MTR_4g023120 48.571 140 54 2 3 124 231 370 3.58e-34 121
MS.gene58316.t1 MTR_1g055175 48.837 129 58 3 2 123 73 200 8.01e-33 114
MS.gene58316.t1 MTR_4g117850 50.806 124 53 2 2 117 250 373 1.78e-32 117
MS.gene58316.t1 MTR_5g097210 52.756 127 50 4 2 121 237 360 2.22e-32 117
MS.gene58316.t1 MTR_4g118360 52.713 129 46 5 6 123 1 125 2.97e-32 110
MS.gene58316.t1 MTR_4g118370 52.000 125 53 3 2 121 135 257 4.40e-32 114
MS.gene58316.t1 MTR_4g117750 56.481 108 40 2 21 121 373 480 6.33e-32 117
MS.gene58316.t1 MTR_4g117750 51.376 109 46 3 20 123 252 358 1.34e-23 94.7
MS.gene58316.t1 MTR_5g023000 60.204 98 32 2 34 124 2 99 1.45e-31 107
MS.gene58316.t1 MTR_1g055165 48.462 130 59 3 2 124 190 318 2.31e-31 113
MS.gene58316.t1 MTR_4g122860 49.606 127 50 3 9 124 197 320 7.01e-31 112
MS.gene58316.t1 MTR_5g070130 50.758 132 56 4 2 124 246 377 9.06e-31 113
MS.gene58316.t1 MTR_4g117780 55.856 111 42 3 21 124 257 367 2.65e-30 111
MS.gene58316.t1 MTR_4g118380 55.455 110 33 3 2 97 248 355 2.85e-30 111
MS.gene58316.t1 MTR_4g117770 55.856 111 42 3 21 124 257 367 3.01e-30 111
MS.gene58316.t1 MTR_4g117810 55.856 111 42 3 21 124 257 367 3.01e-30 111
MS.gene58316.t1 MTR_5g070080 52.000 125 52 3 2 119 257 380 3.20e-30 111
MS.gene58316.t1 MTR_4g023190 51.538 130 54 4 2 122 228 357 7.29e-29 107
MS.gene58316.t1 MTR_4g023440 60.440 91 31 1 39 124 242 332 2.07e-28 105
MS.gene58316.t1 MTR_4g121580 50.877 114 51 2 2 114 230 339 2.39e-28 106
MS.gene58316.t1 MTR_3g034160 54.688 128 50 3 2 123 249 374 2.71e-28 106
MS.gene58316.t1 MTR_4g116330 53.097 113 44 2 21 124 229 341 4.83e-28 105
MS.gene58316.t1 MTR_3g034130 57.000 100 36 2 2 96 249 346 1.15e-27 104
MS.gene58316.t1 MTR_1g055135 48.507 134 54 6 3 124 245 375 1.22e-27 104
MS.gene58316.t1 MTR_4g023150 49.231 130 57 4 2 122 229 358 6.44e-27 102
MS.gene58316.t1 MTR_7g079450 45.312 128 60 4 3 124 14 137 3.60e-26 95.5
MS.gene58316.t1 MTR_4g023200 48.462 130 58 4 2 122 229 358 4.81e-26 100
MS.gene58316.t1 MTR_4g117530 47.458 118 54 3 12 124 139 253 8.14e-26 97.4
MS.gene58316.t1 MTR_4g118400 61.628 86 26 2 40 118 209 294 9.97e-26 98.2
MS.gene58316.t1 MTR_5g070088 46.970 132 61 4 2 124 255 386 4.18e-25 97.8
MS.gene58316.t1 MTR_1g055195 49.219 128 52 6 2 120 351 474 4.93e-25 98.6
MS.gene58316.t1 MTR_7g079400 45.455 132 58 4 5 124 208 337 8.56e-24 94.0
MS.gene58316.t1 MTR_1g055250 46.087 115 54 3 2 111 142 253 3.41e-23 92.0
MS.gene58316.t1 MTR_8g102520 42.969 128 63 4 5 124 197 322 9.90e-23 90.9
MS.gene58316.t1 MTR_8g098830 41.463 123 62 5 1 118 98 215 8.34e-21 84.0
MS.gene58316.t1 MTR_7g079370 43.307 127 63 4 5 124 232 356 9.26e-20 83.2
MS.gene58316.t1 MTR_4g023430 44.231 104 48 2 31 124 119 222 1.11e-19 80.9
MS.gene58316.t1 MTR_7g117720 45.082 122 49 6 8 118 157 271 4.63e-19 80.1
MS.gene58316.t1 MTR_7g079420 40.625 128 65 5 1 119 1014 1139 5.45e-17 75.9
MS.gene58316.t1 MTR_4g114700 49.425 87 34 2 39 115 132 218 1.13e-16 73.2
MS.gene58316.t1 MTR_4g023100 56.364 55 24 0 70 124 65 119 1.18e-12 60.5
MS.gene58316.t1 MTR_8g013005 56.140 57 23 1 70 124 3 59 6.88e-12 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 25 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATTGGACAATTGAAATA+TGG 0.213992 8.2:-65670300 MS.gene58316:CDS
TGTTATGATTTGTGGGTATT+TGG 0.285185 8.2:-65670355 MS.gene58316:CDS
TTGGAGAATTTCTCAGTCTA+TGG 0.288501 8.2:-65670336 MS.gene58316:CDS
TAATTGGACAATTGAAATAT+GGG 0.403350 8.2:-65670299 MS.gene58316:CDS
ATCTTCGGTGTTATGATTTG+TGG 0.417327 8.2:-65670363 MS.gene58316:CDS
AGATCATGGTCAAAATCATC+TGG 0.426340 8.2:+65670382 None:intergenic
GAGTCTCTGCTTTCACTGCC+TGG 0.432512 8.2:-65670073 MS.gene58316:CDS
TGGTCAAAATCATCTGGAAA+TGG 0.450872 8.2:+65670388 None:intergenic
AATTGAAATATGGGTGATGA+AGG 0.467515 8.2:-65670290 MS.gene58316:CDS
TTGGTGAAGTATAATGATAA+AGG 0.492510 8.2:-65670154 MS.gene58316:CDS
TCTTCGGTGTTATGATTTGT+GGG 0.498485 8.2:-65670362 MS.gene58316:CDS
TAATGATAAAGGACAGGAGA+TGG 0.501156 8.2:-65670143 MS.gene58316:CDS
ACTGCCTGGTGACCGTCAGC+AGG 0.501497 8.2:-65670059 MS.gene58316:CDS
AATACAAACAACATTCGTCT+TGG 0.503398 8.2:-65670267 MS.gene58316:CDS
GAAGTATAATGATAAAGGAC+AGG 0.531427 8.2:-65670149 MS.gene58316:CDS
AATCATAACACCGAAGATCA+TGG 0.550232 8.2:+65670368 None:intergenic
GTTGGAAATGACGGTGGTCG+TGG 0.551166 8.2:-65670178 MS.gene58316:CDS
ATAGGGGAAAAGTGAAGGCT+AGG 0.553655 8.2:+65670226 None:intergenic
AGTATATAGGGGAAAAGTGA+AGG 0.557979 8.2:+65670221 None:intergenic
AAATGACGGTGGTCGTGGAT+TGG 0.563207 8.2:-65670173 MS.gene58316:CDS
TAAACCTGCTGACGGTCACC+AGG 0.573679 8.2:+65670055 None:intergenic
GAATCTGAATGCATCTGAAG+TGG 0.574519 8.2:-65670107 MS.gene58316:CDS
GGTGATATTGTTGGAAATGA+CGG 0.595106 8.2:-65670187 MS.gene58316:CDS
CATCTTGTTAAACCTGCTGA+CGG 0.678381 8.2:+65670047 None:intergenic
GATATTGTTGGAAATGACGG+TGG 0.748349 8.2:-65670184 MS.gene58316:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTGGACAATTGAAATA+TGG - chr8.2:65670160-65670179 MS.gene58316:CDS 20.0%
!! TAATTGGACAATTGAAATAT+GGG - chr8.2:65670161-65670180 MS.gene58316:CDS 20.0%
!!! GGAATAGAAATTTTCATAAT+TGG - chr8.2:65670145-65670164 MS.gene58316:CDS 20.0%
! TTGGTGAAGTATAATGATAA+AGG - chr8.2:65670306-65670325 MS.gene58316:CDS 25.0%
!! ACTTTTTGTAGAGTATATAG+GGG + chr8.2:65670253-65670272 None:intergenic 25.0%
!!! CACTTTTTGTAGAGTATATA+GGG + chr8.2:65670254-65670273 None:intergenic 25.0%
AATACAAACAACATTCGTCT+TGG - chr8.2:65670193-65670212 MS.gene58316:CDS 30.0%
AATTGAAATATGGGTGATGA+AGG - chr8.2:65670170-65670189 MS.gene58316:CDS 30.0%
ACAAAAAGTGGTGATATTGT+TGG - chr8.2:65670264-65670283 MS.gene58316:CDS 30.0%
CCTATATACTCTACAAAAAG+TGG - chr8.2:65670252-65670271 MS.gene58316:CDS 30.0%
GAAGTATAATGATAAAGGAC+AGG - chr8.2:65670311-65670330 MS.gene58316:CDS 30.0%
! TGTTATGATTTGTGGGTATT+TGG - chr8.2:65670105-65670124 MS.gene58316:CDS 30.0%
!! ATGATTTTGACCATGATCTT+CGG - chr8.2:65670082-65670101 MS.gene58316:CDS 30.0%
!!! CCACTTTTTGTAGAGTATAT+AGG + chr8.2:65670255-65670274 None:intergenic 30.0%
AATCATAACACCGAAGATCA+TGG + chr8.2:65670095-65670114 None:intergenic 35.0%
AGATCATGGTCAAAATCATC+TGG + chr8.2:65670081-65670100 None:intergenic 35.0%
AGTATATAGGGGAAAAGTGA+AGG + chr8.2:65670242-65670261 None:intergenic 35.0%
GGTGATATTGTTGGAAATGA+CGG - chr8.2:65670273-65670292 MS.gene58316:CDS 35.0%
TAATGATAAAGGACAGGAGA+TGG - chr8.2:65670317-65670336 MS.gene58316:CDS 35.0%
TGGTCAAAATCATCTGGAAA+TGG + chr8.2:65670075-65670094 None:intergenic 35.0%
TTGGAGAATTTCTCAGTCTA+TGG - chr8.2:65670124-65670143 MS.gene58316:CDS 35.0%
!! ATCTTCGGTGTTATGATTTG+TGG - chr8.2:65670097-65670116 MS.gene58316:CDS 35.0%
!! TCTTCGGTGTTATGATTTGT+GGG - chr8.2:65670098-65670117 MS.gene58316:CDS 35.0%
GAATCTGAATGCATCTGAAG+TGG - chr8.2:65670353-65670372 MS.gene58316:CDS 40.0%
GATATTGTTGGAAATGACGG+TGG - chr8.2:65670276-65670295 MS.gene58316:CDS 40.0%
ATAGGGGAAAAGTGAAGGCT+AGG + chr8.2:65670237-65670256 None:intergenic 45.0%
AAATGACGGTGGTCGTGGAT+TGG - chr8.2:65670287-65670306 MS.gene58316:CDS 50.0%
GAGTCTCTGCTTTCACTGCC+TGG - chr8.2:65670387-65670406 MS.gene58316:CDS 55.0%
GTTGGAAATGACGGTGGTCG+TGG - chr8.2:65670282-65670301 MS.gene58316:CDS 55.0%
TAAACCTGCTGACGGTCACC+AGG + chr8.2:65670408-65670427 None:intergenic 55.0%
ACTGCCTGGTGACCGTCAGC+AGG - chr8.2:65670401-65670420 MS.gene58316:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 65670054 65670428 65670054 ID=MS.gene58316
chr8.2 mRNA 65670054 65670428 65670054 ID=MS.gene58316.t1;Parent=MS.gene58316
chr8.2 exon 65670054 65670428 65670054 ID=MS.gene58316.t1.exon1;Parent=MS.gene58316.t1
chr8.2 CDS 65670054 65670428 65670054 ID=cds.MS.gene58316.t1;Parent=MS.gene58316.t1
Gene Sequence

>MS.gene58316

ATGAAACTGTTACAGATACCATTTCCAGATGATTTTGACCATGATCTTCGGTGTTATGATTTGTGGGTATTTGGAGAATTTCTCAGTCTATGGAATAGAAATTTTCATAATTGGACAATTGAAATATGGGTGATGAAGGAATACAAACAACATTCGTCTTGGACTAAGTCTCTTGTAATTCCTAGCCTTCACTTTTCCCCTATATACTCTACAAAAAGTGGTGATATTGTTGGAAATGACGGTGGTCGTGGATTGGTGAAGTATAATGATAAAGGACAGGAGATGGAGTATCTTGAAATGAATCTGAATGCATCTGAAGTGGCTATGTATACAGAGTCTCTGCTTTCACTGCCTGGTGACCGTCAGCAGGTTTAA

Protein sequence

>MS.gene58316.t1

MKLLQIPFPDDFDHDLRCYDLWVFGEFLSLWNRNFHNWTIEIWVMKEYKQHSSWTKSLVIPSLHFSPIYSTKSGDIVGNDGGRGLVKYNDKGQEMEYLEMNLNASEVAMYTESLLSLPGDRQQV