AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033440


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033440.t1 MTR_8g102520 76.526 213 47 1 2 214 113 322 6.81e-114 328
MS.gene033440.t1 MTR_7g079400 64.220 218 71 2 1 214 123 337 1.21e-94 280
MS.gene033440.t1 MTR_8g098830 74.706 170 42 1 19 188 32 200 5.06e-88 259
MS.gene033440.t1 MTR_7g079450 79.286 140 25 1 75 214 2 137 1.33e-76 227
MS.gene033440.t1 MTR_7g079370 57.346 211 89 1 1 211 144 353 7.47e-73 225
MS.gene033440.t1 MTR_2g048580 55.556 216 78 6 4 214 140 342 3.42e-68 213
MS.gene033440.t1 MTR_7g079420 60.440 182 62 5 39 214 967 1144 9.34e-60 202
MS.gene033440.t1 MTR_4g117720 45.045 222 104 8 1 214 167 378 1.52e-50 168
MS.gene033440.t1 MTR_1g055175 43.719 199 108 3 18 214 5 201 4.66e-48 156
MS.gene033440.t1 MTR_6g060650 46.296 216 101 8 1 213 169 372 4.19e-47 159
MS.gene033440.t1 MTR_4g109280 47.475 198 91 8 22 214 170 359 1.98e-45 154
MS.gene033440.t1 MTR_4g116320 43.396 212 109 6 1 209 50 253 1.40e-42 144
MS.gene033440.t1 MTR_4g094365 46.667 195 92 7 19 208 171 358 3.62e-41 143
MS.gene033440.t1 MTR_5g070088 45.455 198 98 6 22 214 194 386 1.05e-40 142
MS.gene033440.t1 MTR_4g116330 45.588 204 88 7 22 214 189 380 1.63e-40 142
MS.gene033440.t1 MTR_5g070080 46.114 193 93 6 22 210 196 381 3.30e-40 141
MS.gene033440.t1 MTR_4g023130 45.128 195 89 6 19 207 124 306 3.80e-40 139
MS.gene033440.t1 MTR_5g097210 44.503 191 97 6 22 209 174 358 4.17e-40 140
MS.gene033440.t1 MTR_4g109270 44.949 198 98 6 22 214 173 364 5.16e-40 140
MS.gene033440.t1 MTR_1g055135 42.268 194 109 2 24 214 182 375 7.05e-40 140
MS.gene033440.t1 MTR_5g070130 42.991 214 106 9 13 214 168 377 8.85e-40 140
MS.gene033440.t1 MTR_3g103020 43.216 199 101 6 22 214 186 378 1.62e-39 139
MS.gene033440.t1 MTR_4g118400 43.386 189 86 6 22 208 125 294 2.23e-39 137
MS.gene033440.t1 MTR_4g023080 44.792 192 88 6 22 207 168 347 2.32e-39 138
MS.gene033440.t1 MTR_1g055195 40.609 197 112 2 22 214 283 478 4.27e-39 140
MS.gene033440.t1 MTR_4g118380 44.633 177 85 4 19 186 185 357 9.64e-39 137
MS.gene033440.t1 MTR_8g433030 43.981 216 102 10 1 210 136 338 4.69e-38 134
MS.gene033440.t1 MTR_8g032390 43.981 216 102 10 1 210 169 371 8.79e-38 135
MS.gene033440.t1 MTR_4g118370 41.579 190 91 3 22 211 88 257 3.77e-37 130
MS.gene033440.t1 MTR_4g117750 43.077 195 101 3 22 209 163 354 7.71e-37 134
MS.gene033440.t1 MTR_4g117750 41.353 133 67 5 86 208 346 477 9.56e-15 72.8
MS.gene033440.t1 MTR_1g055165 42.941 170 93 3 47 214 151 318 9.78e-37 130
MS.gene033440.t1 MTR_4g122850 43.077 195 101 8 19 208 169 358 2.74e-36 131
MS.gene033440.t1 MTR_8g040935 39.713 209 108 9 15 214 9 208 5.60e-36 125
MS.gene033440.t1 MTR_4g021785 43.229 192 92 7 22 207 168 348 9.79e-36 129
MS.gene033440.t1 MTR_3g034180 41.294 201 101 6 22 213 296 488 1.50e-34 128
MS.gene033440.t1 MTR_6g463430 43.367 196 99 7 22 214 16 202 1.91e-34 122
MS.gene033440.t1 MTR_4g117850 38.318 214 118 7 1 207 167 373 4.69e-33 122
MS.gene033440.t1 MTR_3g034160 39.462 223 114 7 3 213 161 374 6.11e-33 122
MS.gene033440.t1 MTR_3g034130 39.888 178 86 5 22 187 182 350 2.47e-32 120
MS.gene033440.t1 MTR_4g023190 41.451 193 98 5 22 208 170 353 5.05e-32 119
MS.gene033440.t1 MTR_4g117530 37.563 197 83 6 20 214 95 253 6.28e-32 116
MS.gene033440.t1 MTR_8g433040 41.089 202 100 6 16 214 179 364 1.77e-31 118
MS.gene033440.t1 MTR_8g032380 41.089 202 100 6 16 214 179 364 1.77e-31 118
MS.gene033440.t1 MTR_4g117770 42.857 203 107 6 17 214 169 367 3.15e-31 117
MS.gene033440.t1 MTR_4g117810 42.857 203 107 6 17 214 169 367 3.15e-31 117
MS.gene033440.t1 MTR_4g117780 41.951 205 110 6 15 214 167 367 3.39e-31 117
MS.gene033440.t1 MTR_4g118360 48.333 120 61 1 90 208 1 120 4.67e-31 110
MS.gene033440.t1 MTR_4g023070 39.806 206 91 8 22 207 171 363 6.00e-31 117
MS.gene033440.t1 MTR_4g023120 40.099 202 96 8 22 207 171 363 6.22e-30 114
MS.gene033440.t1 MTR_1g055250 40.588 170 91 6 22 187 78 241 6.61e-30 113
MS.gene033440.t1 MTR_4g023200 39.583 192 102 7 23 208 171 354 2.04e-29 112
MS.gene033440.t1 MTR_4g023380 36.792 212 100 6 22 208 169 371 2.76e-28 109
MS.gene033440.t1 MTR_4g023150 38.776 196 100 6 22 208 170 354 7.17e-28 108
MS.gene033440.t1 MTR_4g023090 36.232 207 106 7 19 208 181 378 7.60e-28 108
MS.gene033440.t1 MTR_4g117870 37.313 201 71 8 22 214 114 267 2.28e-27 105
MS.gene033440.t1 MTR_4g116330 38.235 204 64 7 22 214 189 341 9.48e-26 102
MS.gene033440.t1 MTR_4g023440 33.824 204 87 6 13 214 175 332 4.41e-23 95.1
MS.gene033440.t1 MTR_5g023000 49.462 93 44 2 124 214 8 99 4.94e-20 81.6
MS.gene033440.t1 MTR_4g117430 46.809 94 43 3 22 115 65 151 1.05e-18 79.7
MS.gene033440.t1 MTR_4g122860 32.323 198 90 8 27 214 157 320 2.56e-18 82.0
MS.gene033440.t1 MTR_7g117720 48.980 98 41 4 119 209 177 272 5.42e-17 77.8
MS.gene033440.t1 MTR_8g013010 46.316 95 41 4 22 116 225 309 7.97e-15 72.0
MS.gene033440.t1 MTR_4g121580 31.401 207 102 9 1 204 170 339 4.21e-14 70.5
MS.gene033440.t1 MTR_6g021880 31.013 158 97 6 1 157 180 326 4.75e-14 70.5
MS.gene033440.t1 MTR_4g114700 32.474 194 65 7 22 205 81 218 4.97e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 54 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATTCTCATTTGCCTTAT+AGG 0.110040 7.2:-25997290 MS.gene033440:CDS
TATTATTGCCTTTGATTTAA+AGG 0.116438 7.2:-25997172 MS.gene033440:CDS
ATTTATGATTTGATGGTATT+TGG 0.157846 7.2:-25997090 MS.gene033440:CDS
GGCAATAATAACATCGCTTT+TGG 0.269149 7.2:+25997185 None:intergenic
CCGACTCTGGGACCACCTTC+AGG 0.271870 7.2:+25997255 None:intergenic
TCTTAGAATTTATGATTTGA+TGG 0.287400 7.2:-25997097 MS.gene033440:CDS
TCTTGAATGGTTCTATTCAT+TGG 0.326345 7.2:-25997227 MS.gene033440:CDS
CTTTCACTCCCCAATGGCAC+TGG 0.329703 7.2:-25996787 MS.gene033440:CDS
GGGCTTGCAATATTCCTATA+AGG 0.347352 7.2:+25997276 None:intergenic
AGAGTTCTAAAATTAAGATA+TGG 0.380976 7.2:-25997041 MS.gene033440:CDS
CTCTGTATACACCGCCATTT+GGG 0.381496 7.2:+25996816 None:intergenic
GAATGGTTCTATTCATTGGC+TGG 0.382596 7.2:-25997223 MS.gene033440:CDS
CACGATTTGTAAAGCATACT+AGG 0.384246 7.2:+25996941 None:intergenic
ACTCTGTATACACCGCCATT+TGG 0.406867 7.2:+25996815 None:intergenic
CGACCACTATTAAAATCATC+TGG 0.411676 7.2:+25997123 None:intergenic
TAAGCTTGCCCAGTGCCATT+GGG 0.413531 7.2:+25996778 None:intergenic
TTGGCGGACTTATCAGTGTA+TGG 0.416802 7.2:-25997071 MS.gene033440:CDS
ACAAATCGTGGTGATATTGT+TGG 0.422110 7.2:-25996928 MS.gene033440:CDS
CTGAAGGTGGTCCCAGAGTC+GGG 0.433638 7.2:-25997254 MS.gene033440:CDS
TTAAGCTTGCCCAGTGCCAT+TGG 0.439492 7.2:+25996777 None:intergenic
CGACTCTGGGACCACCTTCA+GGG 0.442516 7.2:+25997256 None:intergenic
AATACGCAGTGCATTCATCT+TGG 0.467778 7.2:-25997008 MS.gene033440:CDS
TGTCATTTCCTTTAAATCAA+AGG 0.469429 7.2:+25997164 None:intergenic
ATTCAAGAATGACCCGACTC+TGG 0.470304 7.2:+25997242 None:intergenic
GGTATATAGTTCAATTGACT+TGG 0.492098 7.2:-25997355 MS.gene033440:CDS
GAGTTCTAAAATTAAGATAT+GGG 0.492351 7.2:-25997040 MS.gene033440:CDS
GCTAATCCACCATCAGCAAC+TGG 0.495868 7.2:+25996904 None:intergenic
TATGATTTGATGGTATTTGG+CGG 0.504914 7.2:-25997087 MS.gene033440:CDS
TTACTTTATGAGATCCCAAA+TGG 0.511111 7.2:-25996830 MS.gene033440:CDS
AGGAATATTGCAAGCCCTGA+AGG 0.512532 7.2:-25997270 MS.gene033440:CDS
CTGCAAGAGTATCATTCCTA+TGG 0.523562 7.2:-25996859 MS.gene033440:CDS
CTAGTATGCTTTACAAATCG+TGG 0.527578 7.2:-25996940 MS.gene033440:CDS
CCTGAAGGTGGTCCCAGAGT+CGG 0.527660 7.2:-25997255 MS.gene033440:CDS
AGAGTCGGGTCATTCTTGAA+TGG 0.540114 7.2:-25997240 MS.gene033440:CDS
TGAACTATATACCGAAGGAT+TGG 0.540403 7.2:+25997365 None:intergenic
TTCAAGAATGACCCGACTCT+GGG 0.541376 7.2:+25997243 None:intergenic
TTTCACTCCCCAATGGCACT+GGG 0.552642 7.2:-25996786 MS.gene033440:CDS
ATACAATTATACCAATCCTT+CGG 0.554537 7.2:-25997376 MS.gene033440:CDS
CTCATTGAGAGCTAATGAGT+GGG 0.557112 7.2:-25997325 MS.gene033440:CDS
GTTGGACCAGTTGCTGATGG+TGG 0.559934 7.2:-25996910 MS.gene033440:CDS
GATGATTACATGATAGTTGT+GGG 0.567568 7.2:-25997408 None:intergenic
ATTGTTGGACCAGTTGCTGA+TGG 0.574060 7.2:-25996913 MS.gene033440:CDS
AAGCTTGCCCAGTGCCATTG+GGG 0.576980 7.2:+25996779 None:intergenic
CATAAAGTAACGGTCACCAT+AGG 0.590340 7.2:+25996843 None:intergenic
TCTCATTGAGAGCTAATGAG+TGG 0.598081 7.2:-25997326 MS.gene033440:CDS
TGATGATTACATGATAGTTG+TGG 0.600343 7.2:-25997409 None:intergenic
ACGATTTGTAAAGCATACTA+GGG 0.611048 7.2:+25996942 None:intergenic
TCAATTGAACTATATACCGA+AGG 0.631122 7.2:+25997360 None:intergenic
TCTCTGCTTTCACTCCCCAA+TGG 0.640379 7.2:-25996793 MS.gene033440:CDS
TTTGGGATCTCATAAAGTAA+CGG 0.651607 7.2:+25996833 None:intergenic
TTAGCGAAGCTTAATGACAA+AGG 0.665656 7.2:-25996886 MS.gene033440:CDS
AATATTGCAAGCCCTGAAGG+TGG 0.669896 7.2:-25997267 MS.gene033440:CDS
CTTTATGAGATCCCAAATGG+CGG 0.702686 7.2:-25996827 MS.gene033440:CDS
ATGATTACATGATAGTTGTG+GGG 0.750346 7.2:-25997407 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTAGAATTTATGATTTGA+TGG - chr7.2:25997079-25997098 MS.gene033440:CDS 20.0%
!!! AGAGTTCTAAAATTAAGATA+TGG - chr7.2:25997135-25997154 MS.gene033440:CDS 20.0%
!!! ATTTATGATTTGATGGTATT+TGG - chr7.2:25997086-25997105 MS.gene033440:CDS 20.0%
!!! GAGTTCTAAAATTAAGATAT+GGG - chr7.2:25997136-25997155 MS.gene033440:CDS 20.0%
!!! TATTATTGCCTTTGATTTAA+AGG - chr7.2:25997004-25997023 MS.gene033440:CDS 20.0%
! ATACAATTATACCAATCCTT+CGG - chr7.2:25996800-25996819 MS.gene033440:CDS 25.0%
!! TGTCATTTCCTTTAAATCAA+AGG + chr7.2:25997015-25997034 None:intergenic 25.0%
ACGATTTGTAAAGCATACTA+GGG + chr7.2:25997237-25997256 None:intergenic 30.0%
GGTATATAGTTCAATTGACT+TGG - chr7.2:25996821-25996840 MS.gene033440:CDS 30.0%
TCAATTGAACTATATACCGA+AGG + chr7.2:25996819-25996838 None:intergenic 30.0%
TTACTTTATGAGATCCCAAA+TGG - chr7.2:25997346-25997365 MS.gene033440:CDS 30.0%
TTGATTCTCATTTGCCTTAT+AGG - chr7.2:25996886-25996905 MS.gene033440:CDS 30.0%
! TATGATTTGATGGTATTTGG+CGG - chr7.2:25997089-25997108 MS.gene033440:CDS 30.0%
! TCTTGAATGGTTCTATTCAT+TGG - chr7.2:25996949-25996968 MS.gene033440:CDS 30.0%
! TTTGGGATCTCATAAAGTAA+CGG + chr7.2:25997346-25997365 None:intergenic 30.0%
!! CTTCCAGATGATTTTAATAG+TGG - chr7.2:25997050-25997069 MS.gene033440:CDS 30.0%
AAGCATACTAGGGAAAAATC+CGG + chr7.2:25997227-25997246 None:intergenic 35.0%
ACAAATCGTGGTGATATTGT+TGG - chr7.2:25997248-25997267 MS.gene033440:CDS 35.0%
CACGATTTGTAAAGCATACT+AGG + chr7.2:25997238-25997257 None:intergenic 35.0%
CGACCACTATTAAAATCATC+TGG + chr7.2:25997056-25997075 None:intergenic 35.0%
CTAGTATGCTTTACAAATCG+TGG - chr7.2:25997236-25997255 MS.gene033440:CDS 35.0%
TGAACTATATACCGAAGGAT+TGG + chr7.2:25996814-25996833 None:intergenic 35.0%
TTAGCGAAGCTTAATGACAA+AGG - chr7.2:25997290-25997309 MS.gene033440:CDS 35.0%
! GGCAATAATAACATCGCTTT+TGG + chr7.2:25996994-25997013 None:intergenic 35.0%
AATACGCAGTGCATTCATCT+TGG - chr7.2:25997168-25997187 MS.gene033440:CDS 40.0%
CATAAAGTAACGGTCACCAT+AGG + chr7.2:25997336-25997355 None:intergenic 40.0%
CTCATTGAGAGCTAATGAGT+GGG - chr7.2:25996851-25996870 MS.gene033440:CDS 40.0%
CTTTATGAGATCCCAAATGG+CGG - chr7.2:25997349-25997368 MS.gene033440:CDS 40.0%
GGGCTTGCAATATTCCTATA+AGG + chr7.2:25996903-25996922 None:intergenic 40.0%
TCTCATTGAGAGCTAATGAG+TGG - chr7.2:25996850-25996869 MS.gene033440:CDS 40.0%
! GAATGGTTCTATTCATTGGC+TGG - chr7.2:25996953-25996972 MS.gene033440:CDS 40.0%
! TTTTTCTTTTCATCCCGCTC+CGG - chr7.2:25997205-25997224 MS.gene033440:CDS 40.0%
!! CTGCAAGAGTATCATTCCTA+TGG - chr7.2:25997317-25997336 MS.gene033440:CDS 40.0%
AATATTGCAAGCCCTGAAGG+TGG - chr7.2:25996909-25996928 MS.gene033440:CDS 45.0%
ACTCTGTATACACCGCCATT+TGG + chr7.2:25997364-25997383 None:intergenic 45.0%
AGAGTCGGGTCATTCTTGAA+TGG - chr7.2:25996936-25996955 MS.gene033440:CDS 45.0%
AGGAATATTGCAAGCCCTGA+AGG - chr7.2:25996906-25996925 MS.gene033440:CDS 45.0%
ATTCAAGAATGACCCGACTC+TGG + chr7.2:25996937-25996956 None:intergenic 45.0%
ATTGTTGGACCAGTTGCTGA+TGG - chr7.2:25997263-25997282 MS.gene033440:CDS 45.0%
CTCTGTATACACCGCCATTT+GGG + chr7.2:25997363-25997382 None:intergenic 45.0%
TACTAGGGAAAAATCCGGAG+CGG + chr7.2:25997222-25997241 None:intergenic 45.0%
TTCAAGAATGACCCGACTCT+GGG + chr7.2:25996936-25996955 None:intergenic 45.0%
!! TTGGCGGACTTATCAGTGTA+TGG - chr7.2:25997105-25997124 MS.gene033440:CDS 45.0%
ACTAGGGAAAAATCCGGAGC+GGG + chr7.2:25997221-25997240 None:intergenic 50.0%
GCTAATCCACCATCAGCAAC+TGG + chr7.2:25997275-25997294 None:intergenic 50.0%
TCTCTGCTTTCACTCCCCAA+TGG - chr7.2:25997383-25997402 MS.gene033440:CDS 50.0%
TTAAGCTTGCCCAGTGCCAT+TGG + chr7.2:25997402-25997421 None:intergenic 50.0%
TTTCACTCCCCAATGGCACT+GGG - chr7.2:25997390-25997409 MS.gene033440:CDS 50.0%
! TAAGCTTGCCCAGTGCCATT+GGG + chr7.2:25997401-25997420 None:intergenic 50.0%
CTTTCACTCCCCAATGGCAC+TGG - chr7.2:25997389-25997408 MS.gene033440:CDS 55.0%
GTTGGACCAGTTGCTGATGG+TGG - chr7.2:25997266-25997285 MS.gene033440:CDS 55.0%
! AAGCTTGCCCAGTGCCATTG+GGG + chr7.2:25997400-25997419 None:intergenic 55.0%
CCTGAAGGTGGTCCCAGAGT+CGG - chr7.2:25996921-25996940 MS.gene033440:CDS 60.0%
CTGAAGGTGGTCCCAGAGTC+GGG - chr7.2:25996922-25996941 MS.gene033440:CDS 60.0%
!! CGACTCTGGGACCACCTTCA+GGG + chr7.2:25996923-25996942 None:intergenic 60.0%
!! CCGACTCTGGGACCACCTTC+AGG + chr7.2:25996924-25996943 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 25996777 25997421 25996777 ID=MS.gene033440
chr7.2 mRNA 25996777 25997421 25996777 ID=MS.gene033440.t1;Parent=MS.gene033440
chr7.2 exon 25996777 25997421 25996777 ID=MS.gene033440.t1.exon1;Parent=MS.gene033440.t1
chr7.2 CDS 25996777 25997421 25996777 ID=cds.MS.gene033440.t1;Parent=MS.gene033440.t1
Gene Sequence

>MS.gene033440

ATGATAGTTGTGGGGTCATACAAATACAATTATACCAATCCTTCGGTATATAGTTCAATTGACTTGGAGATTTTCTCATTGAGAGCTAATGAGTGGGAACAAATTGAGTTTGATTCTCATTTGCCTTATAGGAATATTGCAAGCCCTGAAGGTGGTCCCAGAGTCGGGTCATTCTTGAATGGTTCTATTCATTGGCTGGTTTATAATTATCAGACCAAAAGCGATGTTATTATTGCCTTTGATTTAAAGGAAATGACACTCTCAGAGATAGCTCTTCCAGATGATTTTAATAGTGGTCGCTCTCTTAGAATTTATGATTTGATGGTATTTGGCGGACTTATCAGTGTATGGATTGTGAAGAGTTCTAAAATTAAGATATGGGTGATGCAAGAATACGCAGTGCATTCATCTTGGACTAAGACTCTTGATTTTTCTTTTCATCCCGCTCCGGATTTTTCCCTAGTATGCTTTACAAATCGTGGTGATATTGTTGGACCAGTTGCTGATGGTGGATTAGCGAAGCTTAATGACAAAGGACAACTGCAAGAGTATCATTCCTATGGTGACCGTTACTTTATGAGATCCCAAATGGCGGTGTATACAGAGTCTCTGCTTTCACTCCCCAATGGCACTGGGCAAGCTTAA

Protein sequence

>MS.gene033440.t1

MIVVGSYKYNYTNPSVYSSIDLEIFSLRANEWEQIEFDSHLPYRNIASPEGGPRVGSFLNGSIHWLVYNYQTKSDVIIAFDLKEMTLSEIALPDDFNSGRSLRIYDLMVFGGLISVWIVKSSKIKIWVMQEYAVHSSWTKTLDFSFHPAPDFSLVCFTNRGDIVGPVADGGLAKLNDKGQLQEYHSYGDRYFMRSQMAVYTESLLSLPNGTGQA