AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40878


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40878.t1 MTR_1g055175 76.687 163 36 1 1 163 41 201 6.03e-87 253
MS.gene40878.t1 MTR_1g055165 76.687 163 36 1 1 163 158 318 9.05e-86 254
MS.gene40878.t1 MTR_1g055135 72.727 165 42 2 1 163 212 375 2.80e-76 232
MS.gene40878.t1 MTR_1g055195 63.804 163 56 3 1 163 319 478 2.36e-61 197
MS.gene40878.t1 MTR_5g097210 65.823 158 50 3 2 159 206 359 8.60e-60 190
MS.gene40878.t1 MTR_1g055250 65.693 137 43 3 1 137 111 243 8.57e-55 176
MS.gene40878.t1 MTR_4g117720 57.988 169 61 7 2 163 213 378 4.03e-51 167
MS.gene40878.t1 MTR_6g060650 55.828 163 67 4 1 162 214 372 5.52e-51 167
MS.gene40878.t1 MTR_4g109280 57.055 163 62 6 4 163 202 359 1.67e-48 160
MS.gene40878.t1 MTR_4g118370 54.375 160 51 5 1 160 120 257 1.18e-46 152
MS.gene40878.t1 MTR_4g117750 56.098 164 64 4 1 159 195 355 2.83e-46 157
MS.gene40878.t1 MTR_4g117750 48.182 110 54 3 54 161 373 481 9.64e-23 93.6
MS.gene40878.t1 MTR_4g094365 51.829 164 75 3 2 163 203 364 9.22e-45 150
MS.gene40878.t1 MTR_3g103020 55.556 162 65 5 5 163 221 378 2.23e-44 150
MS.gene40878.t1 MTR_4g116330 51.786 168 72 6 1 163 217 380 3.00e-44 150
MS.gene40878.t1 MTR_4g116320 53.049 164 66 5 2 160 98 255 8.77e-44 145
MS.gene40878.t1 MTR_6g463430 52.201 159 69 4 6 163 50 202 8.55e-43 141
MS.gene40878.t1 MTR_5g070080 52.976 168 66 7 2 163 226 386 9.52e-42 143
MS.gene40878.t1 MTR_4g122850 49.689 161 76 4 5 163 207 364 3.73e-41 142
MS.gene40878.t1 MTR_5g070088 51.572 159 73 4 1 157 223 379 4.20e-41 142
MS.gene40878.t1 MTR_3g034180 50.617 162 74 5 2 162 332 488 7.91e-41 143
MS.gene40878.t1 MTR_4g118380 54.930 142 53 4 1 133 216 355 1.11e-39 137
MS.gene40878.t1 MTR_8g433030 53.503 157 65 6 4 157 185 336 2.35e-39 136
MS.gene40878.t1 MTR_8g032390 53.503 157 65 6 4 157 218 369 6.15e-39 135
MS.gene40878.t1 MTR_4g117850 50.943 159 69 5 4 156 218 373 7.77e-39 135
MS.gene40878.t1 MTR_8g040935 50.898 167 69 7 5 163 47 208 9.36e-39 131
MS.gene40878.t1 MTR_5g070130 52.258 155 68 5 6 157 219 370 1.13e-38 135
MS.gene40878.t1 MTR_4g109270 50.311 161 73 5 6 163 208 364 1.78e-38 134
MS.gene40878.t1 MTR_4g023130 50.311 161 70 6 5 162 159 312 2.85e-38 132
MS.gene40878.t1 MTR_4g023070 49.419 172 69 6 5 162 202 369 3.37e-38 134
MS.gene40878.t1 MTR_4g023080 50.311 161 70 6 5 162 200 353 3.66e-38 133
MS.gene40878.t1 MTR_4g021785 48.447 161 75 5 5 163 201 355 5.81e-37 130
MS.gene40878.t1 MTR_4g023380 44.324 185 72 7 5 163 200 379 8.53e-37 130
MS.gene40878.t1 MTR_8g433040 51.408 142 63 3 23 163 228 364 5.39e-36 128
MS.gene40878.t1 MTR_8g032380 51.408 142 63 3 23 163 228 364 5.39e-36 128
MS.gene40878.t1 MTR_4g118400 49.686 159 61 6 1 157 153 294 1.00e-35 125
MS.gene40878.t1 MTR_4g023090 45.143 175 75 7 5 162 215 385 1.25e-35 127
MS.gene40878.t1 MTR_3g034160 50.000 162 75 4 2 162 218 374 3.41e-35 126
MS.gene40878.t1 MTR_3g034130 47.651 149 73 4 2 149 218 362 2.73e-34 123
MS.gene40878.t1 MTR_4g023120 46.023 176 71 6 5 163 202 370 2.85e-34 123
MS.gene40878.t1 MTR_7g079400 45.062 162 83 3 6 163 178 337 5.23e-34 122
MS.gene40878.t1 MTR_4g118360 50.000 128 61 3 37 163 1 126 6.47e-34 116
MS.gene40878.t1 MTR_4g023150 47.239 163 75 6 5 161 201 358 1.53e-33 121
MS.gene40878.t1 MTR_4g023190 47.853 163 74 6 5 161 200 357 5.65e-33 120
MS.gene40878.t1 MTR_4g117780 45.122 164 86 4 2 162 204 366 7.72e-33 119
MS.gene40878.t1 MTR_7g079420 44.375 160 84 4 6 163 988 1144 1.37e-32 122
MS.gene40878.t1 MTR_4g117770 45.732 164 85 4 2 162 204 366 1.66e-32 119
MS.gene40878.t1 MTR_4g117810 45.732 164 85 4 2 162 204 366 1.66e-32 119
MS.gene40878.t1 MTR_4g023200 46.012 163 77 6 5 161 201 358 2.77e-32 118
MS.gene40878.t1 MTR_8g102520 40.741 162 94 2 2 163 163 322 1.91e-30 112
MS.gene40878.t1 MTR_4g117530 41.875 160 57 4 5 163 129 253 7.78e-30 109
MS.gene40878.t1 MTR_4g117870 46.923 130 66 2 34 163 141 267 2.01e-29 108
MS.gene40878.t1 MTR_7g079370 43.871 155 80 5 6 158 202 351 1.13e-28 108
MS.gene40878.t1 MTR_5g023000 56.250 96 34 5 72 163 8 99 1.92e-24 91.3
MS.gene40878.t1 MTR_7g079450 40.426 141 78 3 23 163 3 137 2.21e-24 92.4
MS.gene40878.t1 MTR_4g116330 39.286 168 54 6 1 163 217 341 2.71e-23 94.0
MS.gene40878.t1 MTR_2g048580 36.585 164 90 6 2 163 191 342 2.86e-23 94.0
MS.gene40878.t1 MTR_4g023440 38.125 160 55 5 5 162 214 331 1.24e-22 92.0
MS.gene40878.t1 MTR_1g055145 61.039 77 27 1 3 79 129 202 1.31e-21 88.6
MS.gene40878.t1 MTR_4g122860 37.500 168 70 6 1 162 181 319 2.05e-21 89.0
MS.gene40878.t1 MTR_8g098830 37.692 130 78 3 2 131 68 194 5.22e-17 75.5
MS.gene40878.t1 MTR_7g117720 38.710 124 64 4 43 158 153 272 4.36e-15 70.9
MS.gene40878.t1 MTR_4g121580 39.370 127 45 6 5 129 222 318 1.52e-13 67.4
MS.gene40878.t1 MTR_8g013005 55.000 60 26 1 104 163 1 59 1.52e-13 62.0
MS.gene40878.t1 MTR_4g114700 43.182 88 47 2 70 154 131 218 2.30e-13 65.9
MS.gene40878.t1 MTR_4g023100 34.454 119 68 5 49 162 5 118 5.90e-13 62.4
MS.gene40878.t1 MTR_8g013010 56.452 62 21 1 2 63 254 309 7.25e-12 62.4
MS.gene40878.t1 MTR_4g117430 54.237 59 25 1 5 63 96 152 2.88e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGTTGCCTTTCATTTAA+TGG 0.113478 1.4:+46541337 MS.gene40878:CDS
GTTTCAAAGAATCTAATTTA+TGG 0.175078 1.4:+46541411 MS.gene40878:CDS
TTTCTCAGTCTATGGGTTTC+GGG 0.238313 1.4:+46541446 MS.gene40878:CDS
TTAGAGGATTTCTCAGTCTA+TGG 0.277856 1.4:+46541438 MS.gene40878:CDS
ACAAGGGTAAAGTTGATATA+TGG 0.287530 1.4:+46541471 MS.gene40878:CDS
ATTTCTCAGTCTATGGGTTT+CGG 0.288923 1.4:+46541445 MS.gene40878:CDS
TATTCAAAATCATCATCATT+TGG 0.309421 1.4:-46541386 None:intergenic
TTTCAAAGAATCTAATTTAT+GGG 0.327158 1.4:+46541412 MS.gene40878:CDS
TCTAATTTATGGGTATTTAG+AGG 0.356771 1.4:+46541422 MS.gene40878:CDS
ATTCAAAATCATCATCATTT+GGG 0.373726 1.4:-46541385 None:intergenic
TCTTCAATGGGGCTATTCAC+TGG 0.419380 1.4:+46541282 MS.gene40878:CDS
CAAGGGTAAAGTTGATATAT+GGG 0.420388 1.4:+46541472 MS.gene40878:CDS
AATACAAAGTGCAGTCATCT+TGG 0.424763 1.4:+46541504 MS.gene40878:CDS
TGGTAGAGTCAGTCTTCAAT+GGG 0.445013 1.4:+46541270 MS.gene40878:CDS
ATGGTAGAGTCAGTCTTCAA+TGG 0.450181 1.4:+46541269 MS.gene40878:CDS
CAATGGGGCTATTCACTGGT+TGG 0.483990 1.4:+46541286 MS.gene40878:CDS
TTGCCTTTCATTTAATGGAA+AGG 0.488920 1.4:+46541342 MS.gene40878:CDS
TAGAGGATTTCTCAGTCTAT+GGG 0.489737 1.4:+46541439 MS.gene40878:CDS
TTCTCAGTCTATGGGTTTCG+GGG 0.502147 1.4:+46541447 MS.gene40878:CDS
TAAGCTTCCTCATTGTCACT+AGG 0.504127 1.4:-46541737 None:intergenic
TATCCTTTCCATTAAATGAA+AGG 0.507802 1.4:-46541345 None:intergenic
GTTGGAACAGATTACCGTAC+TGG 0.514745 1.4:+46541605 MS.gene40878:CDS
TACAAGGGCAACGTTATGAA+TGG 0.537235 1.4:-46541558 None:intergenic
AAATCATCATCATTTGGGAG+AGG 0.551980 1.4:-46541380 None:intergenic
AAGCTTCCTCATTGTCACTA+GGG 0.552135 1.4:-46541736 None:intergenic
TTCACGAAATGAATTCCGAT+TGG 0.558524 1.4:+46541685 MS.gene40878:CDS
GAACAGATTACCGTACTGGA+TGG 0.571251 1.4:+46541609 MS.gene40878:CDS
ACTCTGTATACATAGCCAAT+CGG 0.573189 1.4:-46541700 None:intergenic
CATATCTCACCCATCCAGTA+CGG 0.584321 1.4:-46541619 None:intergenic
AACAGATTACCGTACTGGAT+GGG 0.623237 1.4:+46541610 MS.gene40878:CDS
TTCACTCCCTAGTGACAATG+AGG 0.626833 1.4:+46541730 MS.gene40878:CDS
TCTCAGTCTATGGGTTTCGG+GGG 0.637073 1.4:+46541448 MS.gene40878:CDS
GCTATTCGTCACGATAGACG+TGG 0.699166 1.4:+46541308 MS.gene40878:CDS
CTATTCGTCACGATAGACGT+GGG 0.735650 1.4:+46541309 MS.gene40878:CDS
TGGGTGAGATATGATGACAA+AGG 0.747254 1.4:+46541629 MS.gene40878:CDS
GGTAGAGTCAGTCTTCAATG+GGG 0.775884 1.4:+46541271 MS.gene40878:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCAAAGAATCTAATTTAT+GGG + chr1.4:46541412-46541431 MS.gene40878:CDS 15.0%
!! ATTCAAAATCATCATCATTT+GGG - chr1.4:46541388-46541407 None:intergenic 20.0%
!! GTTTCAAAGAATCTAATTTA+TGG + chr1.4:46541411-46541430 MS.gene40878:CDS 20.0%
!! TATTCAAAATCATCATCATT+TGG - chr1.4:46541389-46541408 None:intergenic 20.0%
! TATCCTTTCCATTAAATGAA+AGG - chr1.4:46541348-46541367 None:intergenic 25.0%
! TATTGTTGCCTTTCATTTAA+TGG + chr1.4:46541337-46541356 MS.gene40878:CDS 25.0%
! TCTAATTTATGGGTATTTAG+AGG + chr1.4:46541422-46541441 MS.gene40878:CDS 25.0%
ACAAAAAGTGGTGATATTGT+TGG + chr1.4:46541587-46541606 MS.gene40878:CDS 30.0%
TTGCCTTTCATTTAATGGAA+AGG + chr1.4:46541342-46541361 MS.gene40878:CDS 30.0%
! ACTTTTTGTGCAACATACAA+GGG - chr1.4:46541576-46541595 None:intergenic 30.0%
!! ACAAGGGTAAAGTTGATATA+TGG + chr1.4:46541471-46541490 MS.gene40878:CDS 30.0%
!! CAAGGGTAAAGTTGATATAT+GGG + chr1.4:46541472-46541491 MS.gene40878:CDS 30.0%
AAATCATCATCATTTGGGAG+AGG - chr1.4:46541383-46541402 None:intergenic 35.0%
AATACAAAGTGCAGTCATCT+TGG + chr1.4:46541504-46541523 MS.gene40878:CDS 35.0%
ACTCTGTATACATAGCCAAT+CGG - chr1.4:46541703-46541722 None:intergenic 35.0%
ATTTCTCAGTCTATGGGTTT+CGG + chr1.4:46541445-46541464 MS.gene40878:CDS 35.0%
CTTGTATGTTGCACAAAAAG+TGG + chr1.4:46541575-46541594 MS.gene40878:CDS 35.0%
TAGAGGATTTCTCAGTCTAT+GGG + chr1.4:46541439-46541458 MS.gene40878:CDS 35.0%
TTAGAGGATTTCTCAGTCTA+TGG + chr1.4:46541438-46541457 MS.gene40878:CDS 35.0%
TTCACGAAATGAATTCCGAT+TGG + chr1.4:46541685-46541704 MS.gene40878:CDS 35.0%
!! CACTTTTTGTGCAACATACA+AGG - chr1.4:46541577-46541596 None:intergenic 35.0%
AACAGATTACCGTACTGGAT+GGG + chr1.4:46541610-46541629 MS.gene40878:CDS 40.0%
AAGCTTCCTCATTGTCACTA+GGG - chr1.4:46541739-46541758 None:intergenic 40.0%
ATGGTAGAGTCAGTCTTCAA+TGG + chr1.4:46541269-46541288 MS.gene40878:CDS 40.0%
TAAGCTTCCTCATTGTCACT+AGG - chr1.4:46541740-46541759 None:intergenic 40.0%
TACAAGGGCAACGTTATGAA+TGG - chr1.4:46541561-46541580 None:intergenic 40.0%
TGGGTGAGATATGATGACAA+AGG + chr1.4:46541629-46541648 MS.gene40878:CDS 40.0%
TGGTAGAGTCAGTCTTCAAT+GGG + chr1.4:46541270-46541289 MS.gene40878:CDS 40.0%
TTTCTCAGTCTATGGGTTTC+GGG + chr1.4:46541446-46541465 MS.gene40878:CDS 40.0%
CATATCTCACCCATCCAGTA+CGG - chr1.4:46541622-46541641 None:intergenic 45.0%
CTATTCGTCACGATAGACGT+GGG + chr1.4:46541309-46541328 MS.gene40878:CDS 45.0%
GAACAGATTACCGTACTGGA+TGG + chr1.4:46541609-46541628 MS.gene40878:CDS 45.0%
GGTAGAGTCAGTCTTCAATG+GGG + chr1.4:46541271-46541290 MS.gene40878:CDS 45.0%
GTTGGAACAGATTACCGTAC+TGG + chr1.4:46541605-46541624 MS.gene40878:CDS 45.0%
TCTTCAATGGGGCTATTCAC+TGG + chr1.4:46541282-46541301 MS.gene40878:CDS 45.0%
TTCACTCCCTAGTGACAATG+AGG + chr1.4:46541730-46541749 MS.gene40878:CDS 45.0%
TTCTCAGTCTATGGGTTTCG+GGG + chr1.4:46541447-46541466 MS.gene40878:CDS 45.0%
CAATGGGGCTATTCACTGGT+TGG + chr1.4:46541286-46541305 MS.gene40878:CDS 50.0%
GCTATTCGTCACGATAGACG+TGG + chr1.4:46541308-46541327 MS.gene40878:CDS 50.0%
TCTCAGTCTATGGGTTTCGG+GGG + chr1.4:46541448-46541467 MS.gene40878:CDS 50.0%
CTATGGGTTTCGGGGGACAA+GGG + chr1.4:46541455-46541474 MS.gene40878:CDS 55.0%
TCTATGGGTTTCGGGGGACA+AGG + chr1.4:46541454-46541473 MS.gene40878:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 46541269 46541760 46541269 ID=MS.gene40878
chr1.4 mRNA 46541269 46541760 46541269 ID=MS.gene40878.t1;Parent=MS.gene40878
chr1.4 exon 46541269 46541760 46541269 ID=MS.gene40878.t1.exon1;Parent=MS.gene40878.t1
chr1.4 CDS 46541269 46541760 46541269 ID=cds.MS.gene40878.t1;Parent=MS.gene40878.t1
Gene Sequence

>MS.gene40878

ATGGTAGAGTCAGTCTTCAATGGGGCTATTCACTGGTTGGCTATTCGTCACGATAGACGTGGGTATGTTATTGTTGCCTTTCATTTAATGGAAAGGATACTTCTAGAAATTCCTCTCCCAAATGATGATGATTTTGAATATAGTTTCAAAGAATCTAATTTATGGGTATTTAGAGGATTTCTCAGTCTATGGGTTTCGGGGGACAAGGGTAAAGTTGATATATGGGTGATGAAAGAATACAAAGTGCAGTCATCTTGGACTAAAACTCTTGTTCTTACTACTTATGATACCATTCATAACGTTGCCCTTGTATGTTGCACAAAAAGTGGTGATATTGTTGGAACAGATTACCGTACTGGATGGGTGAGATATGATGACAAAGGAGAGTTTCTAGAGCATATCTACTATTGCATAGATTCACGAAATGAATTCCGATTGGCTATGTATACAGAGTCTTTGCTTTCACTCCCTAGTGACAATGAGGAAGCTTAA

Protein sequence

>MS.gene40878.t1

MVESVFNGAIHWLAIRHDRRGYVIVAFHLMERILLEIPLPNDDDFEYSFKESNLWVFRGFLSLWVSGDKGKVDIWVMKEYKVQSSWTKTLVLTTYDTIHNVALVCCTKSGDIVGTDYRTGWVRYDDKGEFLEHIYYCIDSRNEFRLAMYTESLLSLPSDNEEA