AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene041391

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041391.t1 MTR_4g116330 73.190 373 42 3 2 322 14 380 0.0 523
MS.gene041391.t1 MTR_4g116330 64.611 373 35 4 2 322 14 341 3.48e-154 436
MS.gene041391.t1 MTR_3g103020 48.787 371 121 7 10 322 19 378 4.06e-102 305
MS.gene041391.t1 MTR_4g109270 47.967 369 123 9 11 322 8 364 4.98e-99 296
MS.gene041391.t1 MTR_4g117780 46.753 385 124 10 1 322 1 367 1.47e-95 287
MS.gene041391.t1 MTR_4g117770 46.842 380 131 8 1 322 1 367 2.56e-93 282
MS.gene041391.t1 MTR_4g117810 46.842 380 131 8 1 322 1 367 2.56e-93 282
MS.gene041391.t1 MTR_1g055165 48.204 334 140 12 4 322 3 318 3.15e-91 275
MS.gene041391.t1 MTR_4g021785 46.612 369 132 7 4 322 2 355 4.09e-90 273
MS.gene041391.t1 MTR_4g023080 46.467 368 131 8 1 319 1 351 5.37e-90 273
MS.gene041391.t1 MTR_4g023090 45.293 393 128 11 2 321 7 385 1.77e-89 273
MS.gene041391.t1 MTR_4g094365 45.067 375 135 10 6 322 3 364 4.03e-87 266
MS.gene041391.t1 MTR_4g023380 45.078 386 134 11 2 322 7 379 4.30e-83 256
MS.gene041391.t1 MTR_3g034180 43.194 382 148 11 1 321 115 488 3.30e-82 257
MS.gene041391.t1 MTR_4g023130 43.478 368 101 7 1 319 1 310 8.36e-82 250
MS.gene041391.t1 MTR_8g433040 43.583 374 136 12 4 322 11 364 9.92e-80 247
MS.gene041391.t1 MTR_8g032380 43.583 374 136 12 4 322 11 364 9.92e-80 247
MS.gene041391.t1 MTR_4g023190 45.845 373 128 13 2 319 3 356 2.33e-78 243
MS.gene041391.t1 MTR_4g023200 45.014 351 120 12 25 319 24 357 2.48e-78 243
MS.gene041391.t1 MTR_4g118380 46.260 361 118 10 4 293 2 357 3.05e-78 243
MS.gene041391.t1 MTR_1g055135 41.085 387 147 13 2 322 4 375 3.82e-78 243
MS.gene041391.t1 MTR_4g023150 45.187 374 130 13 2 319 3 357 1.27e-77 241
MS.gene041391.t1 MTR_4g023070 43.666 371 141 11 11 319 3 367 6.90e-75 235
MS.gene041391.t1 MTR_4g116320 70.807 161 45 1 160 320 98 256 1.78e-73 227
MS.gene041391.t1 MTR_3g034130 41.081 370 151 11 4 309 2 368 1.07e-72 229
MS.gene041391.t1 MTR_5g070080 40.355 394 138 11 10 322 9 386 1.58e-71 226
MS.gene041391.t1 MTR_4g023440 41.444 374 116 9 2 322 9 332 5.97e-71 223
MS.gene041391.t1 MTR_4g122850 41.803 366 135 16 22 322 12 364 1.28e-69 221
MS.gene041391.t1 MTR_5g097210 40.633 379 145 12 3 320 2 361 2.21e-69 221
MS.gene041391.t1 MTR_4g023120 41.892 370 149 11 11 319 3 367 9.73e-69 219
MS.gene041391.t1 MTR_8g433030 44.753 324 150 12 4 317 33 337 8.49e-67 213
MS.gene041391.t1 MTR_8g032390 43.228 347 155 14 4 317 33 370 3.19e-65 210
MS.gene041391.t1 MTR_8g040935 55.497 191 78 4 136 322 21 208 3.63e-64 202
MS.gene041391.t1 MTR_6g060650 61.111 162 59 2 160 321 215 372 1.50e-59 195
MS.gene041391.t1 MTR_6g060650 50.888 169 60 6 4 159 2 160 1.97e-28 113
MS.gene041391.t1 MTR_4g117850 61.350 163 58 3 160 319 216 376 6.63e-59 194
MS.gene041391.t1 MTR_4g117850 48.125 160 65 5 6 163 19 162 6.14e-35 130
MS.gene041391.t1 MTR_4g117750 40.267 375 137 12 10 318 2 355 9.04e-59 196
MS.gene041391.t1 MTR_4g117750 60.909 110 41 1 210 319 373 480 1.47e-34 131
MS.gene041391.t1 MTR_4g109280 60.494 162 61 2 161 322 201 359 1.31e-58 192
MS.gene041391.t1 MTR_4g109280 51.064 141 56 4 10 150 7 134 7.34e-34 127
MS.gene041391.t1 MTR_4g117870 44.245 278 123 8 66 322 1 267 4.54e-58 188
MS.gene041391.t1 MTR_4g117720 38.564 376 121 11 10 322 50 378 5.37e-58 191
MS.gene041391.t1 MTR_3g034160 58.642 162 62 2 160 321 218 374 1.65e-52 177
MS.gene041391.t1 MTR_3g034160 46.988 166 75 7 1 163 1 156 2.88e-30 118
MS.gene041391.t1 MTR_1g055195 40.892 269 99 7 108 322 216 478 5.32e-51 176
MS.gene041391.t1 MTR_4g122860 38.226 327 131 11 1 322 60 320 3.84e-50 170
MS.gene041391.t1 MTR_4g118400 38.487 304 101 10 62 316 28 294 1.42e-49 167
MS.gene041391.t1 MTR_6g463430 48.500 200 82 5 127 319 14 199 1.88e-49 164
MS.gene041391.t1 MTR_1g055175 52.439 164 73 4 160 322 42 201 3.83e-49 163
MS.gene041391.t1 MTR_7g117720 39.755 327 130 13 1 316 1 271 2.97e-47 160
MS.gene041391.t1 MTR_4g121580 35.657 373 145 14 1 312 1 339 1.86e-46 161
MS.gene041391.t1 MTR_2g048580 33.424 368 158 14 10 322 7 342 1.37e-43 153
MS.gene041391.t1 MTR_8g102520 34.483 348 171 10 5 322 2 322 1.03e-42 150
MS.gene041391.t1 MTR_4g116340 53.216 171 47 5 1 163 26 171 2.63e-42 147
MS.gene041391.t1 MTR_5g070130 51.205 166 77 3 159 322 214 377 7.94e-41 147
MS.gene041391.t1 MTR_5g070130 47.826 161 73 7 1 159 1 152 1.08e-28 114
MS.gene041391.t1 MTR_4g118370 50.311 161 55 5 160 319 121 257 6.02e-40 141
MS.gene041391.t1 MTR_5g070088 49.123 171 80 3 150 319 218 382 1.18e-37 138
MS.gene041391.t1 MTR_5g070088 45.562 169 69 8 8 159 4 166 1.02e-30 119
MS.gene041391.t1 MTR_4g114700 40.826 218 115 5 110 313 1 218 1.40e-36 132
MS.gene041391.t1 MTR_1g055250 48.667 150 69 4 160 309 112 253 4.00e-36 133
MS.gene041391.t1 MTR_4g118360 52.308 130 56 3 195 322 1 126 4.55e-35 124
MS.gene041391.t1 MTR_5g023000 62.000 100 36 1 223 322 2 99 5.04e-35 123
MS.gene041391.t1 MTR_8g013010 50.641 156 62 6 10 159 53 199 2.82e-34 127
MS.gene041391.t1 MTR_8g013010 56.667 60 22 1 160 219 254 309 3.67e-11 63.5
MS.gene041391.t1 MTR_7g079420 37.751 249 103 10 117 322 905 1144 1.87e-33 130
MS.gene041391.t1 MTR_4g117530 40.323 186 65 4 139 322 112 253 4.49e-33 123
MS.gene041391.t1 MTR_7g079400 45.562 169 81 5 160 322 174 337 1.20e-30 118
MS.gene041391.t1 MTR_7g079370 45.000 160 78 5 160 316 198 350 5.44e-27 108
MS.gene041391.t1 MTR_7g079370 41.284 109 51 6 40 146 16 113 1.58e-11 64.7
MS.gene041391.t1 MTR_8g098830 40.964 166 89 5 128 289 34 194 3.68e-26 103
MS.gene041391.t1 MTR_1g055145 33.621 232 86 9 11 236 33 202 5.18e-25 102
MS.gene041391.t1 MTR_7g079450 45.775 142 70 4 181 322 3 137 1.61e-24 97.1
MS.gene041391.t1 MTR_4g107820 27.224 371 182 13 11 314 36 385 5.98e-23 98.6
MS.gene041391.t1 MTR_4g107780 27.224 371 182 13 11 314 36 385 6.65e-23 98.6
MS.gene041391.t1 MTR_4g107820 27.224 371 182 13 11 314 36 385 6.87e-23 98.2
MS.gene041391.t1 MTR_4g107780 27.224 371 182 13 11 314 36 385 6.87e-23 98.2
MS.gene041391.t1 MTR_1g097490 26.158 367 183 14 11 314 49 390 1.70e-22 97.1
MS.gene041391.t1 MTR_1g094210 26.849 365 187 13 9 314 55 398 2.13e-22 97.1
MS.gene041391.t1 MTR_5g022420 28.024 339 170 14 7 286 23 346 3.05e-22 96.3
MS.gene041391.t1 MTR_1g094210 26.923 338 174 11 9 289 55 376 3.66e-22 96.3
MS.gene041391.t1 MTR_4g109290 39.623 159 55 6 4 159 2 122 7.92e-21 88.6
MS.gene041391.t1 MTR_4g109290 67.273 55 16 1 268 322 136 188 1.62e-15 73.9
MS.gene041391.t1 MTR_8g101580 25.263 380 188 16 6 314 40 394 8.01e-21 92.4
MS.gene041391.t1 MTR_4g108820 29.530 298 149 13 9 269 40 313 1.40e-20 90.9
MS.gene041391.t1 MTR_4g117430 36.184 152 49 2 118 221 3 154 1.46e-20 87.0
MS.gene041391.t1 MTR_4g023430 54.878 82 36 1 242 322 141 222 3.86e-20 87.4
MS.gene041391.t1 MTR_4g023430 60.000 85 29 3 2 85 35 115 7.29e-19 84.0
MS.gene041391.t1 MTR_4g122840 42.029 138 42 4 186 322 164 264 1.56e-19 87.0
MS.gene041391.t1 MTR_5g069120 42.188 128 61 6 21 146 1 117 1.74e-19 84.7
MS.gene041391.t1 MTR_1g056710 32.597 181 108 4 1 178 24 193 2.51e-19 88.2
MS.gene041391.t1 MTR_8g013005 64.516 62 18 2 262 322 1 59 3.54e-19 80.1
MS.gene041391.t1 MTR_1g055055 27.208 283 157 10 11 254 26 298 6.89e-19 86.3
MS.gene041391.t1 MTR_3g010440 28.094 299 175 12 7 285 22 300 8.09e-19 85.9
MS.gene041391.t1 MTR_3g009310 27.063 303 152 10 5 249 35 326 1.38e-18 85.9
MS.gene041391.t1 MTR_5g011200 25.817 306 161 11 3 250 9 306 1.22e-17 83.2
MS.gene041391.t1 MTR_3g096250 28.963 328 184 16 9 314 45 345 2.66e-17 81.6
MS.gene041391.t1 MTR_3g036430 29.151 271 149 11 9 250 18 274 6.10e-17 80.9
MS.gene041391.t1 MTR_3g010970 27.336 289 142 11 13 250 50 321 6.13e-17 81.3
MS.gene041391.t1 MTR_1g097330 28.660 321 158 12 9 271 14 321 3.08e-16 79.3
MS.gene041391.t1 MTR_1g097560 27.778 324 151 15 7 271 32 331 3.50e-16 79.0
MS.gene041391.t1 MTR_3g010620 26.370 292 148 10 9 251 34 307 4.96e-16 78.6
MS.gene041391.t1 MTR_2g021150 25.000 368 207 16 7 314 10 368 4.99e-16 78.2
MS.gene041391.t1 MTR_7g007300 24.090 357 218 12 4 314 96 445 7.44e-16 78.2
MS.gene041391.t1 MTR_6g065980 26.132 287 150 8 6 245 11 282 1.26e-15 77.0
MS.gene041391.t1 MTR_5g057710 26.821 302 143 10 11 250 46 331 2.98e-15 76.3
MS.gene041391.t1 MTR_7g067680 25.874 286 156 9 7 250 45 316 3.96e-15 75.9
MS.gene041391.t1 MTR_2g078900 25.413 303 150 11 7 250 29 314 4.13e-15 75.5
MS.gene041391.t1 MTR_5g008530 70.175 57 15 2 11 67 18 72 6.74e-15 68.9
MS.gene041391.t1 MTR_3g007300 27.473 364 166 16 7 314 45 366 7.94e-15 74.7
MS.gene041391.t1 MTR_8g467140 26.837 313 140 12 10 254 31 322 8.32e-15 74.3
MS.gene041391.t1 MTR_2g055970 26.012 346 172 12 10 288 210 538 1.33e-14 74.7
MS.gene041391.t1 MTR_7g055830 26.243 362 191 13 14 317 16 359 1.88e-14 73.6
MS.gene041391.t1 MTR_3g007330 25.581 301 149 8 7 249 44 327 2.02e-14 73.6
MS.gene041391.t1 MTR_5g057790 26.471 306 139 11 11 250 46 331 2.91e-14 73.2
MS.gene041391.t1 MTR_4g023100 60.345 58 19 1 264 321 65 118 4.85e-14 68.2
MS.gene041391.t1 MTR_1g058190 26.071 280 160 11 10 250 34 305 5.33e-14 72.4
MS.gene041391.t1 MTR_8g073550 31.288 163 105 5 5 165 24 181 7.71e-14 70.5
MS.gene041391.t1 MTR_3g024110 25.753 299 156 11 4 250 94 378 1.44e-13 71.2
MS.gene041391.t1 MTR_1g055070 34.706 170 93 7 3 159 41 205 1.84e-13 70.9
MS.gene041391.t1 MTR_6g042600 25.681 257 154 9 3 254 28 252 2.37e-13 70.5
MS.gene041391.t1 MTR_3g009240 27.333 300 139 12 7 245 31 312 4.06e-13 69.7
MS.gene041391.t1 MTR_3g436150 35.714 168 89 10 7 162 28 188 4.10e-13 69.7
MS.gene041391.t1 MTR_3g072470 24.625 333 155 13 7 254 15 336 5.31e-13 69.7
MS.gene041391.t1 MTR_3g010870 26.984 252 140 10 7 250 33 248 8.73e-13 68.2
MS.gene041391.t1 MTR_6g065510 23.333 330 209 10 10 315 22 331 2.17e-12 67.4
MS.gene041391.t1 MTR_3g024150 24.518 363 199 13 13 314 37 385 2.51e-12 67.4
MS.gene041391.t1 MTR_3g006570 25.914 301 155 13 7 250 17 306 2.68e-12 67.4
MS.gene041391.t1 MTR_3g010520 26.481 287 148 12 9 250 23 291 2.91e-12 67.0
MS.gene041391.t1 MTR_3g013740 27.687 307 148 15 11 250 44 343 3.56e-12 67.0
MS.gene041391.t1 MTR_3g023930 23.512 336 164 12 9 285 32 333 3.79e-12 66.6
MS.gene041391.t1 MTR_7g407070 32.335 167 99 7 7 163 28 190 4.29e-12 66.6
MS.gene041391.t1 MTR_2g104340 30.714 140 92 3 10 149 13 147 5.51e-12 66.6
MS.gene041391.t1 MTR_3g030330 24.483 290 161 10 9 250 18 297 5.78e-12 66.2
MS.gene041391.t1 MTR_0760s0010 25.862 290 162 10 16 260 52 333 7.17e-12 66.2
MS.gene041391.t1 MTR_5g023570 38.017 121 62 4 5 125 31 138 7.48e-12 62.8
MS.gene041391.t1 MTR_3g030250 24.915 293 153 10 9 250 35 311 8.67e-12 65.9
MS.gene041391.t1 MTR_4g031770 28.571 133 87 1 11 143 4 128 1.03e-11 65.5
MS.gene041391.t1 MTR_6g004660 31.868 182 87 8 1 159 3 170 1.40e-11 65.1
MS.gene041391.t1 MTR_3g007410 23.936 376 185 13 14 315 55 403 1.49e-11 65.1
MS.gene041391.t1 MTR_7g022670 26.766 269 151 10 7 245 19 271 1.81e-11 64.7
MS.gene041391.t1 MTR_3g058470 26.885 305 169 14 9 271 35 327 2.05e-11 64.7
MS.gene041391.t1 MTR_2g006360 25.970 335 191 14 14 313 49 361 2.11e-11 64.7
MS.gene041391.t1 MTR_3g014540 31.765 170 82 7 7 162 25 174 3.33e-11 63.9
MS.gene041391.t1 MTR_2g021155 34.591 159 86 9 6 159 31 176 3.51e-11 64.7
MS.gene041391.t1 MTR_3g010430 30.303 165 96 7 3 159 28 181 4.99e-11 63.5
MS.gene041391.t1 MTR_1g054725 33.333 138 84 4 7 143 16 146 5.86e-11 63.2
MS.gene041391.t1 MTR_7g056390 22.687 335 160 10 7 254 14 336 5.89e-11 63.5
MS.gene041391.t1 MTR_3g014570 30.120 166 90 7 7 162 25 174 5.92e-11 63.2
MS.gene041391.t1 MTR_3g011060 31.492 181 113 7 5 178 22 198 7.13e-11 63.2
MS.gene041391.t1 MTR_3g437520 40.909 88 42 2 14 101 26 103 9.79e-11 62.8
MS.gene041391.t1 MTR_3g006670 33.129 163 90 8 5 159 43 194 9.85e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041391.t1 AT3G06240 24.372 398 210 11 3 314 27 419 3.51e-21 94.0
MS.gene041391.t1 AT3G16210 23.145 337 171 14 10 288 4 310 4.45e-11 63.5

Find 71 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAATTGCATTTGATTTAA+TGG 0.081393 8.2:+12756598 MS.gene041391:CDS
TTGGAGAATTTCTCAGTTTA+TGG 0.121537 8.2:+12756693 MS.gene041391:CDS
CATGGGGATTTCATAAAATT+AGG 0.147989 8.2:-12756178 None:intergenic
AATTGTAATTTGTGGGTATT+TGG 0.170321 8.2:+12756674 MS.gene041391:CDS
TGTAGAGCATATTGGGTAAA+AGG 0.214064 8.2:-12756828 None:intergenic
TTCCATAATCTATATGGATT+TGG 0.215138 8.2:+12756349 MS.gene041391:CDS
AATAGTTTCCATAATCTATA+TGG 0.238904 8.2:+12756343 MS.gene041391:CDS
ACCCAAATCCATATAGATTA+TGG 0.264769 8.2:-12756351 None:intergenic
TGTCATTATACTTCAGTAAC+TGG 0.304036 8.2:-12756886 None:intergenic
TCCATAATCTATATGGATTT+GGG 0.308248 8.2:+12756350 MS.gene041391:CDS
ATGGGGATTTCATAAAATTA+GGG 0.319960 8.2:-12756177 None:intergenic
TGGAAACTATTTGAATGATT+AGG 0.321894 8.2:-12756331 None:intergenic
TTCTAGAGCATGGATCATTC+TGG 0.332187 8.2:+12756918 MS.gene041391:CDS
TGTTTGTAAACTCCATTTGA+TGG 0.333995 8.2:-12756295 None:intergenic
AGAGTAGGGTCACTCTTTAA+TGG 0.338450 8.2:+12756530 MS.gene041391:intron
CTAATGAAAGAGTTGAAATA+TGG 0.360862 8.2:+12756729 MS.gene041391:CDS
TCTTTAATGGAGCTATTCAT+TGG 0.379072 8.2:+12756543 MS.gene041391:CDS
TGGAGAATTTCTCAGTTTAT+GGG 0.382420 8.2:+12756694 MS.gene041391:CDS
TGGGACGGTCCACTTCCATT+TGG 0.399776 8.2:+12756938 MS.gene041391:CDS
TCAGTTTATGGGCCAACGAT+TGG 0.400528 8.2:+12756705 MS.gene041391:CDS
TAAAACTCTACGAGAGTGTT+TGG 0.409691 8.2:-12756065 None:intergenic
CAGTTTATGGGCCAACGATT+GGG 0.413913 8.2:+12756706 MS.gene041391:CDS
TTCATTTGGAATCCATCAAA+TGG 0.418802 8.2:+12756283 MS.gene041391:CDS
AACTAATAATTGTAATTTGT+GGG 0.419232 8.2:+12756667 MS.gene041391:CDS
AGAGTATAAATGTCATCAAC+AGG 0.427177 8.2:-12756803 None:intergenic
ATTTCATAAAATTAGGGATC+GGG 0.432363 8.2:-12756171 None:intergenic
TCAAATGCGTTTGTAAGCCT+TGG 0.432851 8.2:+12755987 MS.gene041391:CDS
CAACTAATAATTGTAATTTG+TGG 0.434909 8.2:+12756666 MS.gene041391:CDS
TAATGGAGCTATTCATTGGT+TGG 0.434950 8.2:+12756547 MS.gene041391:CDS
TCGACACGAACTTGAATTTG+TGG 0.455672 8.2:-12756227 None:intergenic
TTAGGCTTGCACATTGTCAC+CGG 0.455704 8.2:-12757002 None:intergenic
GGATTCCAAATGAAGAGGTC+TGG 0.463765 8.2:-12756274 None:intergenic
TTAGAGATGCCTTTACCTGA+TGG 0.465602 8.2:+12756632 MS.gene041391:CDS
ATCAATTCAAGAGGCAGATA+AGG 0.472857 8.2:-12755920 None:intergenic
ACTCTGTATACACGGTCACT+TGG 0.479905 8.2:-12756964 None:intergenic
ATAAAATTAGGGATCGGGTT+GGG 0.482578 8.2:-12756166 None:intergenic
TCAAATTCTGTTGAGGTTGG+CGG 0.482772 8.2:+12755946 MS.gene041391:CDS
GAGTCTTTGCTTTCACTCCC+CGG 0.489307 8.2:+12756983 MS.gene041391:CDS
TCTAGAGCATGGATCATTCT+GGG 0.502981 8.2:+12756919 MS.gene041391:CDS
AATTACAATTATTAGTTGCA+TGG 0.507420 8.2:-12756661 None:intergenic
GTTGCATGGTCAAAACCATC+AGG 0.515710 8.2:-12756647 None:intergenic
AATACAAAGTGCATTCGTCT+TGG 0.518219 8.2:+12756762 MS.gene041391:CDS
CATAAAATTAGGGATCGGGT+TGG 0.519790 8.2:-12756167 None:intergenic
ACGGTCACTTGGGATCCAAA+TGG 0.522598 8.2:-12756953 None:intergenic
AGTTCAAATTCTGTTGAGGT+TGG 0.526320 8.2:+12755943 MS.gene041391:CDS
TAATGAAAGAGTTGAAATAT+GGG 0.539316 8.2:+12756730 MS.gene041391:CDS
ACTTGGGATCCAAATGGAAG+TGG 0.547144 8.2:-12756947 None:intergenic
CTCTGTATACACGGTCACTT+GGG 0.553016 8.2:-12756963 None:intergenic
GATTTCATAAAATTAGGGAT+CGG 0.554332 8.2:-12756172 None:intergenic
TGGTCAAAACCATCAGGTAA+AGG 0.562825 8.2:-12756641 None:intergenic
TAGGCTTGCACATTGTCACC+GGG 0.563652 8.2:-12757001 None:intergenic
AAATCTAAAGATAGTGTAGT+CGG 0.565833 8.2:-12756106 None:intergenic
ATTTGAACTATCAATTCAAG+AGG 0.571814 8.2:-12755929 None:intergenic
TGATAGTTCAAATTCTGTTG+AGG 0.572350 8.2:+12755939 MS.gene041391:CDS
AGAGGACAGCTTCTAGAGCA+TGG 0.578862 8.2:+12756908 MS.gene041391:CDS
TTTGAATGATTAGGAGACAA+AGG 0.589171 8.2:-12756322 None:intergenic
TCTTTCATTAGCCCAATCGT+TGG 0.591565 8.2:-12756717 None:intergenic
TTGATGGATTCCAAATGAAG+AGG 0.599601 8.2:-12756279 None:intergenic
AACTCTACGAGAGTGTTTGG+CGG 0.602805 8.2:-12756062 None:intergenic
TCCACTACATGAAAATCACA+TGG 0.604640 8.2:-12756196 None:intergenic
ACAAATTCAAGTTCGTGTCG+AGG 0.613489 8.2:+12756229 MS.gene041391:CDS
TCAAGAGGCAGATAAGGCTG+TGG 0.625428 8.2:-12755914 None:intergenic
GTCATTATACTTCAGTAACT+GGG 0.631722 8.2:-12756885 None:intergenic
AAATGCGAATTTGCAAAGTG+AGG 0.641941 8.2:-12756031 None:intergenic
GAGCATGGATCATTCTGGGA+CGG 0.652794 8.2:+12756923 MS.gene041391:CDS
CCACTACATGAAAATCACAT+GGG 0.654062 8.2:-12756195 None:intergenic
CACTACATGAAAATCACATG+GGG 0.668813 8.2:-12756194 None:intergenic
AAGCAAAGACTCTGTATACA+CGG 0.681751 8.2:-12756972 None:intergenic
TTACTGAAGTATAATGACAG+AGG 0.710690 8.2:+12756890 MS.gene041391:CDS
TTAGAGATAAGAGAAAACCA+AGG 0.720239 8.2:-12756004 None:intergenic
AGGCTTGCACATTGTCACCG+GGG 0.736430 8.2:-12757000 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTAATAATTGTAATTTGT+GGG + chr8.2:12756667-12756686 MS.gene041391:CDS 15.0%
!!! TATAATTGCATTTGATTTAA+TGG + chr8.2:12756598-12756617 MS.gene041391:CDS 15.0%
!! AATAGTTTCCATAATCTATA+TGG + chr8.2:12756343-12756362 MS.gene041391:CDS 20.0%
!! AATTACAATTATTAGTTGCA+TGG - chr8.2:12756664-12756683 None:intergenic 20.0%
!! CAACTAATAATTGTAATTTG+TGG + chr8.2:12756666-12756685 MS.gene041391:CDS 20.0%
!! TAATGAAAGAGTTGAAATAT+GGG + chr8.2:12756730-12756749 MS.gene041391:CDS 20.0%
! AAATCTAAAGATAGTGTAGT+CGG - chr8.2:12756109-12756128 None:intergenic 25.0%
! AATGAAAAAAACCTCAAATG+CGG - chr8.2:12756443-12756462 None:intergenic 25.0%
! ATGGGGATTTCATAAAATTA+GGG - chr8.2:12756180-12756199 None:intergenic 25.0%
! ATTTCATAAAATTAGGGATC+GGG - chr8.2:12756174-12756193 None:intergenic 25.0%
! ATTTGAACTATCAATTCAAG+AGG - chr8.2:12755932-12755951 None:intergenic 25.0%
! CTAATGAAAGAGTTGAAATA+TGG + chr8.2:12756729-12756748 MS.gene041391:CDS 25.0%
! GATTTCATAAAATTAGGGAT+CGG - chr8.2:12756175-12756194 None:intergenic 25.0%
! TCAATTAAGAGATGATTACT+TGG + chr8.2:12756382-12756401 MS.gene041391:intron 25.0%
! TCCATAATCTATATGGATTT+GGG + chr8.2:12756350-12756369 MS.gene041391:CDS 25.0%
! TGGAAACTATTTGAATGATT+AGG - chr8.2:12756334-12756353 None:intergenic 25.0%
! TTCCATAATCTATATGGATT+TGG + chr8.2:12756349-12756368 MS.gene041391:CDS 25.0%
! TTTCATTGAGAGATAATACT+TGG + chr8.2:12756456-12756475 MS.gene041391:intron 25.0%
!! AATTGTAATTTGTGGGTATT+TGG + chr8.2:12756674-12756693 MS.gene041391:CDS 25.0%
!!! ATCTTTAGATTTTGAAGCAT+TGG + chr8.2:12756117-12756136 MS.gene041391:CDS 25.0%
!!! CATTTTTTGTAGAGCATATT+GGG - chr8.2:12756838-12756857 None:intergenic 25.0%
AACAGAATAGTGAAGAACAT+AGG - chr8.2:12756507-12756526 None:intergenic 30.0%
ACAAAAAATGGTGACATTCT+TGG + chr8.2:12756848-12756867 MS.gene041391:CDS 30.0%
ACAGAATAGTGAAGAACATA+GGG - chr8.2:12756506-12756525 None:intergenic 30.0%
ACCCAAATCCATATAGATTA+TGG - chr8.2:12756354-12756373 None:intergenic 30.0%
AGAGTATAAATGTCATCAAC+AGG - chr8.2:12756806-12756825 None:intergenic 30.0%
ATTAAGAGATGATTACTTGG+TGG + chr8.2:12756385-12756404 MS.gene041391:intron 30.0%
CATGGGGATTTCATAAAATT+AGG - chr8.2:12756181-12756200 None:intergenic 30.0%
CCAATATGCTCTACAAAAAA+TGG + chr8.2:12756836-12756855 MS.gene041391:CDS 30.0%
GTCATTATACTTCAGTAACT+GGG - chr8.2:12756888-12756907 None:intergenic 30.0%
TAATACTTGGAATGAACTTG+AGG + chr8.2:12756469-12756488 MS.gene041391:intron 30.0%
TCTTTAATGGAGCTATTCAT+TGG + chr8.2:12756543-12756562 MS.gene041391:CDS 30.0%
TGATAGTTCAAATTCTGTTG+AGG + chr8.2:12755939-12755958 MS.gene041391:CDS 30.0%
TGGAGAATTTCTCAGTTTAT+GGG + chr8.2:12756694-12756713 MS.gene041391:CDS 30.0%
TGTCATTATACTTCAGTAAC+TGG - chr8.2:12756889-12756908 None:intergenic 30.0%
TGTTTGTAAACTCCATTTGA+TGG - chr8.2:12756298-12756317 None:intergenic 30.0%
TTACTGAAGTATAATGACAG+AGG + chr8.2:12756890-12756909 MS.gene041391:CDS 30.0%
TTAGAGATAAGAGAAAACCA+AGG - chr8.2:12756007-12756026 None:intergenic 30.0%
TTGGAGAATTTCTCAGTTTA+TGG + chr8.2:12756693-12756712 MS.gene041391:CDS 30.0%
TTTGAATGATTAGGAGACAA+AGG - chr8.2:12756325-12756344 None:intergenic 30.0%
! TTCATTTGGAATCCATCAAA+TGG + chr8.2:12756283-12756302 MS.gene041391:CDS 30.0%
!!! CCATTTTTTGTAGAGCATAT+TGG - chr8.2:12756839-12756858 None:intergenic 30.0%
AAATGCGAATTTGCAAAGTG+AGG - chr8.2:12756034-12756053 None:intergenic 35.0%
AAGCAAAGACTCTGTATACA+CGG - chr8.2:12756975-12756994 None:intergenic 35.0%
AATACAAAGTGCATTCGTCT+TGG + chr8.2:12756762-12756781 MS.gene041391:CDS 35.0%
ACTATTCTGTTCACAGAGTA+GGG + chr8.2:12756516-12756535 MS.gene041391:intron 35.0%
AGTTCAAATTCTGTTGAGGT+TGG + chr8.2:12755943-12755962 MS.gene041391:CDS 35.0%
ATAAAATTAGGGATCGGGTT+GGG - chr8.2:12756169-12756188 None:intergenic 35.0%
ATCAATTCAAGAGGCAGATA+AGG - chr8.2:12755923-12755942 None:intergenic 35.0%
CACTACATGAAAATCACATG+GGG - chr8.2:12756197-12756216 None:intergenic 35.0%
CAGAATAGTGAAGAACATAG+GGG - chr8.2:12756505-12756524 None:intergenic 35.0%
CCACTACATGAAAATCACAT+GGG - chr8.2:12756198-12756217 None:intergenic 35.0%
TAATGGAGCTATTCATTGGT+TGG + chr8.2:12756547-12756566 MS.gene041391:CDS 35.0%
TCCACTACATGAAAATCACA+TGG - chr8.2:12756199-12756218 None:intergenic 35.0%
TGTAGAGCATATTGGGTAAA+AGG - chr8.2:12756831-12756850 None:intergenic 35.0%
! ATTTTCCAGACCTCTTCATT+TGG + chr8.2:12756269-12756288 MS.gene041391:CDS 35.0%
!! TAAAACTCTACGAGAGTGTT+TGG - chr8.2:12756068-12756087 None:intergenic 35.0%
!! TTGATGGATTCCAAATGAAG+AGG - chr8.2:12756282-12756301 None:intergenic 35.0%
AGAGTAGGGTCACTCTTTAA+TGG + chr8.2:12756530-12756549 MS.gene041391:intron 40.0%
CACTATTCTGTTCACAGAGT+AGG + chr8.2:12756515-12756534 MS.gene041391:intron 40.0%
CATAAAATTAGGGATCGGGT+TGG - chr8.2:12756170-12756189 None:intergenic 40.0%
TAATGAGCTATCCGCATTTG+AGG + chr8.2:12756429-12756448 MS.gene041391:intron 40.0%
TCAAATGCGTTTGTAAGCCT+TGG + chr8.2:12755987-12756006 MS.gene041391:CDS 40.0%
TCAAATTCTGTTGAGGTTGG+CGG + chr8.2:12755946-12755965 MS.gene041391:CDS 40.0%
TCGACACGAACTTGAATTTG+TGG - chr8.2:12756230-12756249 None:intergenic 40.0%
TCTTTCATTAGCCCAATCGT+TGG - chr8.2:12756720-12756739 None:intergenic 40.0%
TGGTCAAAACCATCAGGTAA+AGG - chr8.2:12756644-12756663 None:intergenic 40.0%
TTAGAGATGCCTTTACCTGA+TGG + chr8.2:12756632-12756651 MS.gene041391:CDS 40.0%
! ACAAATTCAAGTTCGTGTCG+AGG + chr8.2:12756229-12756248 MS.gene041391:CDS 40.0%
! CCCATGTGATTTTCATGTAG+TGG + chr8.2:12756195-12756214 MS.gene041391:CDS 40.0%
! TCTAGAGCATGGATCATTCT+GGG + chr8.2:12756919-12756938 MS.gene041391:CDS 40.0%
!! TTCTAGAGCATGGATCATTC+TGG + chr8.2:12756918-12756937 MS.gene041391:CDS 40.0%
ACTCTGTATACACGGTCACT+TGG - chr8.2:12756967-12756986 None:intergenic 45.0%
ACTTGGGATCCAAATGGAAG+TGG - chr8.2:12756950-12756969 None:intergenic 45.0%
CAGTTTATGGGCCAACGATT+GGG + chr8.2:12756706-12756725 MS.gene041391:CDS 45.0%
CTCTGTATACACGGTCACTT+GGG - chr8.2:12756966-12756985 None:intergenic 45.0%
GGATTCCAAATGAAGAGGTC+TGG - chr8.2:12756277-12756296 None:intergenic 45.0%
GTTGCATGGTCAAAACCATC+AGG - chr8.2:12756650-12756669 None:intergenic 45.0%
TCAGTTTATGGGCCAACGAT+TGG + chr8.2:12756705-12756724 MS.gene041391:CDS 45.0%
TTAGGCTTGCACATTGTCAC+CGG - chr8.2:12757005-12757024 None:intergenic 45.0%
!! AACTCTACGAGAGTGTTTGG+CGG - chr8.2:12756065-12756084 None:intergenic 45.0%
ACGGTCACTTGGGATCCAAA+TGG - chr8.2:12756956-12756975 None:intergenic 50.0%
GAGTCTTTGCTTTCACTCCC+CGG + chr8.2:12756983-12757002 MS.gene041391:CDS 50.0%
TAGGCTTGCACATTGTCACC+GGG - chr8.2:12757004-12757023 None:intergenic 50.0%
TCAAGAGGCAGATAAGGCTG+TGG - chr8.2:12755917-12755936 None:intergenic 50.0%
! GAGCATGGATCATTCTGGGA+CGG + chr8.2:12756923-12756942 MS.gene041391:CDS 50.0%
!! AGAGGACAGCTTCTAGAGCA+TGG + chr8.2:12756908-12756927 MS.gene041391:CDS 50.0%
AGGCTTGCACATTGTCACCG+GGG - chr8.2:12757003-12757022 None:intergenic 55.0%
TGGGACGGTCCACTTCCATT+TGG + chr8.2:12756938-12756957 MS.gene041391:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 12755896 12757024 12755896 ID=MS.gene041391
chr8.2 mRNA 12755896 12757024 12755896 ID=MS.gene041391.t1;Parent=MS.gene041391
chr8.2 exon 12755896 12756371 12755896 ID=MS.gene041391.t1.exon1;Parent=MS.gene041391.t1
chr8.2 CDS 12755896 12756371 12755896 ID=cds.MS.gene041391.t1;Parent=MS.gene041391.t1
chr8.2 exon 12756532 12757024 12756532 ID=MS.gene041391.t1.exon2;Parent=MS.gene041391.t1
chr8.2 CDS 12756532 12757024 12756532 ID=cds.MS.gene041391.t1;Parent=MS.gene041391.t1
Gene Sequence

>MS.gene041391

ATGAAGAAGAAGACGAATCCACAGCCTTATCTGCCTCTTGAATTGATAGTTCAAATTCTGTTGAGGTTGGCGGTGAAGTCTCTTCTTCGTTTCAAATGCGTTTGTAAGCCTTGGTTTTCTCTTATCTCTAATGATCCTCACTTTGCAAATTCGCATTTTCAAATAACCGCCAAACACTCTCGTAGAGTTTTATTCATGTCAATTCATGTTCCGACTACACTATCTTTAGATTTTGAAGCATTGGATTGTGATAATGTTGTTTCTGAAATACCCAACCCGATCCCTAATTTTATGAAATCCCCATGTGATTTTCATGTAGTGGATTATCTTGCCACAAATTCAAGTTCGTGTCGAGGATTTATATTTCTGCACAATTTTCCAGACCTCTTCATTTGGAATCCATCAAATGGAGTTTACAAACAAATACCTTTGTCTCCTAATCATTCAAATAGTTTCCATAATCTATATGGATTTGGGTATGTATGATCAATTAAGAGATGATTACTTGGTGGTTTCTGTAACATCGCAAAAATTAATGAGCTATCCGCATTTGAGGTTTTTTTCATTGAGAGATAATACTTGGAATGAACTTGAGGCTGCTCACTTCCCCTATGTTCTTCACTATTCTGTTCACAGAGTAGGGTCACTCTTTAATGGAGCTATTCATTGGTTGGTTTTTCATAGTGATATAAAGAGTCTAGTTATAATTGCATTTGATTTAATGGAAAGAAAGCTTTTAGAGATGCCTTTACCTGATGGTTTTGACCATGCAACTAATAATTGTAATTTGTGGGTATTTGGAGAATTTCTCAGTTTATGGGCCAACGATTGGGCTAATGAAAGAGTTGAAATATGGGTGATGAACGAATACAAAGTGCATTCGTCTTGGACTAAAACTCTTGTTCTTCCTGTTGATGACATTTATACTCTTTCCTTTTACCCAATATGCTCTACAAAAAATGGTGACATTCTTGGAACAGACAGTGTTACCCAGTTACTGAAGTATAATGACAGAGGACAGCTTCTAGAGCATGGATCATTCTGGGACGGTCCACTTCCATTTGGATCCCAAGTGACCGTGTATACAGAGTCTTTGCTTTCACTCCCCGGTGACAATGTGCAAGCCTAA
Protein sequence

>MS.gene041391.t1

MKKKTNPQPYLPLELIVQILLRLAVKSLLRFKCVCKPWFSLISNDPHFANSHFQITAKHSRRVLFMSIHVPTTLSLDFEALDCDNVVSEIPNPIPNFMKSPCDFHVVDYLATNSSSCRGFIFLHNFPDLFIWNPSNGVYKQIPLSPNHSNSFHNLYGFGVGSLFNGAIHWLVFHSDIKSLVIIAFDLMERKLLEMPLPDGFDHATNNCNLWVFGEFLSLWANDWANERVEIWVMNEYKVHSSWTKTLVLPVDDIYTLSFYPICSTKNGDILGTDSVTQLLKYNDRGQLLEHGSFWDGPLPFGSQVTVYTESLLSLPGDNVQA