AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000038

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000038.t1 MTR_3g034160 89.744 312 31 1 1 312 64 374 0.0 556
MS.gene000038.t1 MTR_3g034180 86.581 313 41 1 1 313 178 489 0.0 555
MS.gene000038.t1 MTR_3g034130 86.288 299 41 0 1 299 64 362 0.0 526
MS.gene000038.t1 MTR_3g103020 63.694 314 99 6 3 312 75 377 3.61e-131 378
MS.gene000038.t1 MTR_4g117720 62.539 323 95 11 1 312 70 377 1.32e-123 359
MS.gene000038.t1 MTR_6g060650 60.870 322 105 6 1 312 62 372 1.48e-120 351
MS.gene000038.t1 MTR_4g109270 56.918 318 113 8 1 312 64 363 9.35e-108 318
MS.gene000038.t1 MTR_4g109280 60.000 310 105 8 6 312 65 358 1.00e-107 318
MS.gene000038.t1 MTR_4g122850 56.051 314 121 9 6 313 62 364 5.14e-106 314
MS.gene000038.t1 MTR_4g117770 57.235 311 113 5 9 313 71 367 7.35e-106 313
MS.gene000038.t1 MTR_4g117810 57.235 311 113 5 9 313 71 367 7.35e-106 313
MS.gene000038.t1 MTR_4g117780 54.487 312 120 6 9 313 71 367 1.81e-100 300
MS.gene000038.t1 MTR_4g094365 52.665 319 128 7 1 313 63 364 4.98e-100 298
MS.gene000038.t1 MTR_4g117850 55.375 307 112 9 7 306 85 373 5.99e-100 298
MS.gene000038.t1 MTR_4g118380 54.305 302 106 6 6 286 67 357 1.84e-97 291
MS.gene000038.t1 MTR_4g023080 53.822 314 119 8 1 312 64 353 3.32e-97 291
MS.gene000038.t1 MTR_4g023090 50.150 333 129 9 1 313 71 386 4.65e-96 289
MS.gene000038.t1 MTR_4g021785 51.911 314 126 7 1 312 64 354 4.66e-95 285
MS.gene000038.t1 MTR_5g097210 50.164 305 136 6 7 310 71 360 3.10e-93 281
MS.gene000038.t1 MTR_4g023380 49.267 341 113 9 1 313 71 379 6.63e-93 281
MS.gene000038.t1 MTR_5g070080 50.151 331 127 8 6 312 69 385 4.59e-91 276
MS.gene000038.t1 MTR_4g116330 50.161 311 134 7 8 312 84 379 6.05e-89 271
MS.gene000038.t1 MTR_4g023070 49.697 330 130 11 1 312 58 369 2.55e-88 269
MS.gene000038.t1 MTR_5g070130 49.688 320 135 10 6 312 70 376 4.02e-87 266
MS.gene000038.t1 MTR_4g117870 49.682 314 110 7 1 313 1 267 4.85e-87 262
MS.gene000038.t1 MTR_4g023120 49.544 329 134 10 1 313 58 370 6.14e-87 265
MS.gene000038.t1 MTR_1g055135 48.113 318 137 9 7 312 73 374 1.76e-85 261
MS.gene000038.t1 MTR_8g433040 50.641 312 121 9 9 313 79 364 2.75e-84 258
MS.gene000038.t1 MTR_8g032380 50.641 312 121 9 9 313 79 364 2.75e-84 258
MS.gene000038.t1 MTR_4g023130 54.982 271 98 7 44 312 64 312 5.30e-84 256
MS.gene000038.t1 MTR_4g117750 48.571 315 133 7 7 312 64 358 4.11e-83 259
MS.gene000038.t1 MTR_4g117750 60.550 109 41 1 205 311 373 481 1.92e-34 131
MS.gene000038.t1 MTR_4g023190 50.943 318 124 12 1 311 65 357 9.76e-83 254
MS.gene000038.t1 MTR_4g023200 49.686 318 129 12 1 311 65 358 1.85e-81 251
MS.gene000038.t1 MTR_5g070088 46.084 332 144 9 6 313 66 386 5.85e-80 248
MS.gene000038.t1 MTR_8g032390 49.191 309 113 10 7 308 99 370 1.44e-79 246
MS.gene000038.t1 MTR_1g055195 46.519 316 138 7 3 310 183 475 6.56e-79 248
MS.gene000038.t1 MTR_4g023150 49.057 318 131 12 1 311 65 358 7.05e-79 244
MS.gene000038.t1 MTR_4g023440 46.965 313 112 7 1 313 74 332 7.32e-78 241
MS.gene000038.t1 MTR_4g118400 46.844 301 112 8 16 307 33 294 4.39e-74 230
MS.gene000038.t1 MTR_4g116320 56.696 224 82 6 91 310 43 255 1.18e-72 225
MS.gene000038.t1 MTR_8g040935 57.746 213 76 5 107 312 2 207 1.19e-71 221
MS.gene000038.t1 MTR_7g079400 43.182 308 152 8 14 312 43 336 2.60e-71 224
MS.gene000038.t1 MTR_8g013010 57.870 216 74 6 1 214 109 309 3.75e-68 215
MS.gene000038.t1 MTR_7g079370 43.438 320 145 11 7 307 48 350 1.89e-67 215
MS.gene000038.t1 MTR_4g118370 44.643 280 123 6 33 310 8 257 1.17e-66 209
MS.gene000038.t1 MTR_8g433030 54.626 227 84 8 87 308 125 337 1.72e-65 209
MS.gene000038.t1 MTR_4g121580 45.752 306 116 13 7 303 75 339 2.84e-61 200
MS.gene000038.t1 MTR_4g116330 42.122 311 120 8 8 312 84 340 1.22e-59 194
MS.gene000038.t1 MTR_8g102520 40.836 311 142 10 7 312 48 321 2.75e-57 187
MS.gene000038.t1 MTR_1g055165 39.482 309 123 7 7 312 70 317 3.03e-56 185
MS.gene000038.t1 MTR_6g463430 50.952 210 87 5 109 313 4 202 5.09e-55 178
MS.gene000038.t1 MTR_1g055175 48.241 199 93 5 118 312 8 200 1.29e-52 172
MS.gene000038.t1 MTR_4g117430 51.724 174 61 5 46 217 3 155 1.19e-48 160
MS.gene000038.t1 MTR_4g117530 39.100 289 116 10 28 312 20 252 1.57e-48 163
MS.gene000038.t1 MTR_1g055250 42.130 216 113 6 86 300 49 253 3.08e-47 162
MS.gene000038.t1 MTR_4g114700 40.520 269 99 6 42 304 5 218 4.41e-45 154
MS.gene000038.t1 MTR_4g116340 49.162 179 75 5 7 180 80 247 1.39e-42 147
MS.gene000038.t1 MTR_2g048580 33.762 311 156 12 10 312 73 341 3.44e-41 146
MS.gene000038.t1 MTR_4g118360 58.268 127 47 3 190 312 1 125 2.65e-40 137
MS.gene000038.t1 MTR_7g079420 33.808 281 131 9 45 312 905 1143 1.11e-36 140
MS.gene000038.t1 MTR_4g122860 42.132 197 72 8 125 313 158 320 6.03e-36 132
MS.gene000038.t1 MTR_8g098830 42.529 174 86 5 114 282 30 194 4.17e-35 127
MS.gene000038.t1 MTR_5g023000 67.391 92 30 0 221 312 7 98 6.92e-34 120
MS.gene000038.t1 MTR_6g034720 45.000 160 69 5 40 196 31 174 1.10e-29 112
MS.gene000038.t1 MTR_7g117720 52.083 144 53 7 7 145 52 184 4.16e-29 112
MS.gene000038.t1 MTR_7g117720 49.505 101 46 3 213 308 172 272 7.37e-18 82.0
MS.gene000038.t1 MTR_7g079450 41.333 150 63 5 174 312 1 136 2.18e-25 99.0
MS.gene000038.t1 MTR_5g069120 45.736 129 61 2 6 127 51 177 1.04e-22 93.2
MS.gene000038.t1 MTR_1g068875 29.572 257 148 12 3 243 52 291 1.97e-22 96.7
MS.gene000038.t1 MTR_4g122840 42.647 136 40 4 181 313 164 264 1.27e-21 92.4
MS.gene000038.t1 MTR_4g023430 56.098 82 33 1 235 313 141 222 6.65e-20 86.7
MS.gene000038.t1 MTR_2g028160 27.817 284 157 11 21 274 86 351 1.96e-19 88.6
MS.gene000038.t1 MTR_2g426020 25.373 268 164 9 31 276 88 341 5.48e-19 87.0
MS.gene000038.t1 MTR_0667s0010 25.256 293 176 11 4 268 57 334 1.04e-18 86.3
MS.gene000038.t1 MTR_2g028130 27.273 253 146 10 37 264 100 339 1.80e-18 85.5
MS.gene000038.t1 MTR_2g024110 25.180 278 174 11 10 269 63 324 2.31e-18 85.1
MS.gene000038.t1 MTR_2g426070 30.594 219 125 8 57 261 129 334 9.60e-18 83.6
MS.gene000038.t1 MTR_4g133170 28.794 257 149 13 2 242 51 289 1.06e-17 83.2
MS.gene000038.t1 MTR_2g031640 26.667 225 140 6 33 242 81 295 1.30e-17 82.8
MS.gene000038.t1 MTR_1g068835 27.049 244 143 11 19 243 65 292 2.52e-17 82.0
MS.gene000038.t1 MTR_8g013005 67.241 58 19 0 255 312 1 58 2.97e-17 75.1
MS.gene000038.t1 MTR_2g021770 28.086 324 190 14 17 308 93 405 8.33e-17 80.9
MS.gene000038.t1 MTR_2g025960 25.830 271 158 10 39 282 1 255 1.49e-16 79.0
MS.gene000038.t1 MTR_2g026010 27.027 259 165 10 32 276 78 326 1.82e-16 79.7
MS.gene000038.t1 MTR_4g070550 26.667 285 161 12 3 250 53 326 2.67e-16 79.3
MS.gene000038.t1 MTR_2g426090 25.092 271 163 11 29 276 86 339 4.93e-16 78.6
MS.gene000038.t1 MTR_7g070440 28.105 306 164 14 17 285 71 357 1.18e-15 77.0
MS.gene000038.t1 MTR_3g083640 26.937 271 164 9 44 305 157 402 1.70e-15 77.0
MS.gene000038.t1 MTR_1g055200 47.059 102 45 5 42 139 5 101 2.01e-15 71.2
MS.gene000038.t1 MTR_2g078870 31.841 201 105 11 44 238 58 232 2.22e-15 75.9
MS.gene000038.t1 MTR_5g037680 25.962 312 189 12 13 305 93 381 4.53e-15 75.9
MS.gene000038.t1 MTR_3g083010 25.468 267 161 8 37 276 102 357 5.26e-15 75.5
MS.gene000038.t1 MTR_2g031720 24.026 308 195 11 2 280 43 340 6.93e-15 75.1
MS.gene000038.t1 MTR_7g028660 28.253 269 167 9 21 273 88 346 7.63e-15 74.7
MS.gene000038.t1 MTR_1g068860 26.582 237 147 9 21 243 71 294 1.32e-14 73.9
MS.gene000038.t1 MTR_4g117550 27.244 312 173 14 17 308 94 371 1.63e-14 73.9
MS.gene000038.t1 MTR_2g031680 27.111 225 138 7 32 242 80 292 1.76e-14 73.6
MS.gene000038.t1 MTR_2g425980 29.386 228 130 9 54 262 119 334 5.33e-14 72.4
MS.gene000038.t1 MTR_4g030830 24.422 303 166 13 44 305 113 393 5.68e-14 72.4
MS.gene000038.t1 MTR_8g099460 23.920 301 180 10 4 283 56 328 1.88e-13 70.9
MS.gene000038.t1 MTR_4g031770 28.054 221 126 11 31 242 93 289 1.90e-13 70.5
MS.gene000038.t1 MTR_4g023100 63.158 57 19 1 257 313 65 119 2.42e-13 66.2
MS.gene000038.t1 MTR_0044s0300 28.293 205 110 8 44 238 138 315 3.37e-13 70.1
MS.gene000038.t1 MTR_3g010870 25.606 289 174 11 32 305 122 384 3.41e-13 69.7
MS.gene000038.t1 MTR_4g055060 27.668 253 141 11 9 243 94 322 6.69e-13 68.9
MS.gene000038.t1 MTR_1g054725 28.139 231 134 11 21 238 95 306 7.81e-13 68.9
MS.gene000038.t1 MTR_3g010970 27.007 274 157 11 41 305 152 391 8.83e-13 68.6
MS.gene000038.t1 MTR_1g094210 27.237 257 146 11 40 284 151 378 1.42e-12 68.2
MS.gene000038.t1 MTR_6g065980 29.577 213 120 10 44 252 111 297 1.60e-12 67.8
MS.gene000038.t1 MTR_3g036290 29.952 207 112 10 41 243 117 294 2.19e-12 67.4
MS.gene000038.t1 MTR_2g461450 26.222 225 140 7 34 251 99 304 2.52e-12 67.0
MS.gene000038.t1 MTR_2g021150 26.744 258 165 9 15 264 87 328 2.71e-12 67.0
MS.gene000038.t1 MTR_7g070880 27.119 236 139 9 56 264 146 375 3.07e-12 67.0
MS.gene000038.t1 MTR_7g056390 26.814 317 152 12 41 306 130 417 3.79e-12 67.0
MS.gene000038.t1 MTR_2g021050 25.726 241 147 10 37 264 89 310 3.86e-12 66.6
MS.gene000038.t1 MTR_8g464770 25.000 308 196 12 17 307 88 377 3.96e-12 66.6
MS.gene000038.t1 MTR_3g072470 25.723 311 162 12 41 305 130 417 4.69e-12 66.6
MS.gene000038.t1 MTR_3g036430 25.743 202 115 9 44 238 96 269 5.40e-12 66.2
MS.gene000038.t1 MTR_7g070830 25.523 239 139 9 56 264 146 375 6.57e-12 66.2
MS.gene000038.t1 MTR_1g055145 45.946 74 38 2 157 229 130 202 7.46e-12 65.1
MS.gene000038.t1 MTR_8g467670 24.837 306 190 12 17 303 88 372 7.63e-12 65.9
MS.gene000038.t1 MTR_6g053260 27.751 209 130 7 34 238 429 620 7.68e-12 66.2
MS.gene000038.t1 MTR_3g006670 28.182 220 134 9 31 242 133 336 8.84e-12 65.9
MS.gene000038.t1 MTR_2g043410 28.947 228 125 8 40 251 133 339 8.97e-12 65.9
MS.gene000038.t1 MTR_4g028510 26.667 285 155 11 59 310 139 402 9.23e-12 65.9
MS.gene000038.t1 MTR_2g021950 25.545 321 189 18 17 306 92 393 1.19e-11 65.5
MS.gene000038.t1 MTR_2g078900 29.670 182 101 8 59 238 153 309 1.39e-11 65.1
MS.gene000038.t1 MTR_1g094210 27.092 251 142 11 40 278 151 372 1.86e-11 64.7
MS.gene000038.t1 MTR_3g030250 25.000 280 160 11 46 305 130 379 1.95e-11 64.7
MS.gene000038.t1 MTR_2g104340 25.649 308 153 13 47 308 117 394 2.12e-11 64.7
MS.gene000038.t1 MTR_6g090180 25.795 283 164 10 54 306 168 434 2.26e-11 64.7
MS.gene000038.t1 MTR_3g013740 27.404 208 127 8 44 242 150 342 2.78e-11 64.3
MS.gene000038.t1 MTR_2g426060 24.715 263 153 12 33 264 88 336 3.22e-11 63.9
MS.gene000038.t1 MTR_7g056290 26.408 284 145 10 41 285 134 392 3.26e-11 63.9
MS.gene000038.t1 MTR_7g056503 25.589 297 175 13 40 305 116 397 3.38e-11 63.9
MS.gene000038.t1 MTR_7g056003 27.273 231 131 7 41 251 118 331 3.40e-11 63.9
MS.gene000038.t1 MTR_6g027910 26.168 214 132 6 38 238 106 306 5.02e-11 63.2
MS.gene000038.t1 MTR_5g097680 25.088 283 163 12 59 308 159 425 6.03e-11 63.2
MS.gene000038.t1 MTR_3g009310 26.289 194 114 6 56 243 157 327 6.43e-11 63.2
MS.gene000038.t1 MTR_5g096630 24.221 289 169 11 54 308 140 412 6.85e-11 63.2
MS.gene000038.t1 MTR_3g023930 26.471 272 156 11 44 305 130 367 7.36e-11 62.8
MS.gene000038.t1 MTR_7g445890 25.957 235 141 9 41 251 87 312 7.67e-11 62.8
MS.gene000038.t1 MTR_8g012090 26.697 221 107 10 57 263 139 318 8.02e-11 62.4
MS.gene000038.t1 MTR_4g109290 59.615 52 21 0 261 312 136 187 8.59e-11 60.5
MS.gene000038.t1 MTR_0464s0020 26.613 248 148 10 24 251 119 352 8.71e-11 62.8
MS.gene000038.t1 MTR_0129s0070 24.920 313 195 13 20 307 94 391 9.42e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000038.t1 AT3G06240 28.205 234 132 10 39 260 163 372 2.08e-16 79.7
MS.gene000038.t1 AT4G12560 26.117 291 196 10 40 313 90 378 3.34e-14 73.2
MS.gene000038.t1 AT4G12560 26.117 291 196 10 40 313 90 378 3.34e-14 73.2
MS.gene000038.t1 AT4G12560 26.117 291 196 10 40 313 90 378 3.34e-14 73.2

Find 72 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTTGCATTCATATAATA+AGG 0.164755 3.1:+24692714 None:intergenic
GATGATTACTTGGTGGTATT+AGG 0.195715 3.1:-24692861 MS.gene000038:CDS
TTGGAGAATTTCTAAGTCTA+TGG 0.196739 3.1:-24692521 MS.gene000038:CDS
CCATGTTGTCTGTATGGTTT+TGG 0.202696 3.1:-24692900 MS.gene000038:CDS
GATTGTGATTTGTGGATATT+TGG 0.226311 3.1:-24692540 MS.gene000038:CDS
AGGATGGAACAATTGAAATA+TGG 0.245076 3.1:-24692491 MS.gene000038:CDS
GAGTCTCTGCTTTCACTTCC+TGG 0.246781 3.1:-24692243 MS.gene000038:CDS
TGGATATTGAGCATCTAAAT+TGG 0.288449 3.1:+24692920 None:intergenic
GTTCTTCAAGCATTTATCTA+TGG 0.293765 3.1:-24692986 MS.gene000038:CDS
GATGAAATATTATCTTCTAT+TGG 0.305333 3.1:+24692816 None:intergenic
AAAGTAGGGACGCTCTTTAA+TGG 0.309047 3.1:-24692684 MS.gene000038:CDS
ATGAAATATTATCTTCTATT+GGG 0.319010 3.1:+24692817 None:intergenic
AGATAATATTTCATCACATT+TGG 0.320818 3.1:-24692808 MS.gene000038:CDS
AAGCGATGCTTCAAAATCTA+TGG 0.326989 3.1:+24693115 None:intergenic
ATGATTACTTGGTGGTATTA+GGG 0.352995 3.1:-24692860 MS.gene000038:CDS
ATTAAAGAGCGTCCCTACTT+TGG 0.358272 3.1:+24692686 None:intergenic
TATTGTTGGCTTTGATTTAG+TGG 0.370118 3.1:-24692616 MS.gene000038:CDS
TTCAAGATTAGAATAAGATT+CGG 0.374154 3.1:+24693046 None:intergenic
TTAAAGAGCGTCCCTACTTT+GGG 0.374951 3.1:+24692687 None:intergenic
TCAAGATTAGAATAAGATTC+GGG 0.381250 3.1:+24693047 None:intergenic
TCTTTAATGGGACTATTCAT+TGG 0.384035 3.1:-24692671 MS.gene000038:CDS
TGGAAATGTCATCAATAGAT+AGG 0.410119 3.1:+24692421 None:intergenic
TGTTTGTGATGTCCGGTTGA+TGG 0.414503 3.1:+24692960 None:intergenic
CAATATCCATGTTGTCTGTA+TGG 0.424134 3.1:-24692906 MS.gene000038:CDS
AACCTATAGATTGTGATTTG+TGG 0.425796 3.1:-24692548 MS.gene000038:CDS
AAGTAGGGACGCTCTTTAAT+GGG 0.430028 3.1:-24692683 MS.gene000038:CDS
GGATGGAACAATTGAAATAT+GGG 0.445935 3.1:-24692490 MS.gene000038:CDS
CATTTGCATTCATATAATAA+GGG 0.448554 3.1:+24692715 None:intergenic
AGGTATTTGTTTGTGATGTC+CGG 0.452958 3.1:+24692953 None:intergenic
AATACAAAGTGCATTCGTCT+TGG 0.453817 3.1:-24692458 MS.gene000038:CDS
AATCACAATCTATAGGTTCA+TGG 0.456461 3.1:+24692553 None:intergenic
ATACAAAGTGCATTCGTCTT+GGG 0.456469 3.1:-24692457 MS.gene000038:CDS
GGTTCATGGTCAAAACCATC+TGG 0.456773 3.1:+24692567 None:intergenic
TGGGTCTTTACTCGTATCAT+AGG 0.459170 3.1:+24692836 None:intergenic
ATCCACAAATCACAATCTAT+AGG 0.460799 3.1:+24692546 None:intergenic
TCTAAATTGGAAGGAGGAAA+AGG 0.467688 3.1:+24692933 None:intergenic
ATTGGAACAAACGGTGGCAC+TGG 0.477184 3.1:-24692354 MS.gene000038:CDS
TTATCAATGGATGTTATTGT+TGG 0.479970 3.1:-24692630 MS.gene000038:CDS
CTCTGTATACATAGCCACTA+GGG 0.483092 3.1:+24692263 None:intergenic
TGGAGAATTTCTAAGTCTAT+GGG 0.490268 3.1:-24692520 MS.gene000038:CDS
TGATTATACTTCACCAATCC+AGG 0.492432 3.1:+24692741 None:intergenic
CCATTCAAGAGATGATTACT+TGG 0.492530 3.1:-24692871 MS.gene000038:CDS
GGGGTTCACATGGTTCGTTA+TGG 0.498590 3.1:+24692283 None:intergenic
ATTTCTAAGTCTATGGGCTA+TGG 0.507502 3.1:-24692514 MS.gene000038:CDS
TGGTCAAAACCATCTGGAAA+AGG 0.508561 3.1:+24692573 None:intergenic
TATCTATGGAATCCATCAAC+CGG 0.509941 3.1:-24692972 MS.gene000038:CDS
GCAAATGATGATCCCAAAGT+AGG 0.511115 3.1:-24692699 MS.gene000038:CDS
TGGAAAGATATTGAGTGTCC+TGG 0.515552 3.1:-24692759 MS.gene000038:CDS
TTTCATCGAGAGCTAATACA+TGG 0.517874 3.1:-24692779 MS.gene000038:CDS
CCAAGTAATCATCTCTTGAA+TGG 0.518868 3.1:+24692871 None:intergenic
AGTCTATGGGCTATGGAGGA+TGG 0.532601 3.1:-24692507 MS.gene000038:CDS
GAAATTAAAAGTTCATGTAG+AGG 0.538733 3.1:-24693026 MS.gene000038:CDS
ATTCGGGAGGCATAAAATCA+AGG 0.540291 3.1:+24693063 None:intergenic
GGTGGTATTATTGGAACAAA+CGG 0.557309 3.1:-24692363 MS.gene000038:CDS
TTCAAGAGATGATTACTTGG+TGG 0.560339 3.1:-24692868 MS.gene000038:CDS
CCAAAACCATACAGACAACA+TGG 0.562242 3.1:+24692900 None:intergenic
TTGGCTTTGATTTAGTGGAA+AGG 0.565737 3.1:-24692611 MS.gene000038:CDS
CAAATGATGATCCCAAAGTA+GGG 0.565983 3.1:-24692698 MS.gene000038:CDS
AACAAACGGTGGCACTGGAT+TGG 0.570072 3.1:-24692349 MS.gene000038:CDS
TAAATTTGCTCACTGTCACC+AGG 0.573577 3.1:+24692225 None:intergenic
AGATATTGAGTGTCCTGGAT+TGG 0.574538 3.1:-24692754 MS.gene000038:CDS
TCTAAGTCTATGGGCTATGG+AGG 0.577648 3.1:-24692511 MS.gene000038:CDS
TATTGAGCATCTAAATTGGA+AGG 0.583373 3.1:+24692924 None:intergenic
ACTGGATTGGTGAAGTATGA+TGG 0.588559 3.1:-24692336 MS.gene000038:CDS
ACTCTGTATACATAGCCACT+AGG 0.618200 3.1:+24692262 None:intergenic
TGAGCATCTAAATTGGAAGG+AGG 0.655232 3.1:+24692927 None:intergenic
CGAACCATGTGAACCCCTAG+TGG 0.657467 3.1:-24692277 MS.gene000038:CDS
AGATTAGAATAAGATTCGGG+AGG 0.659056 3.1:+24693050 None:intergenic
TCTGTATACATAGCCACTAG+GGG 0.693139 3.1:+24692264 None:intergenic
ATAGCCACTAGGGGTTCACA+TGG 0.697094 3.1:+24692273 None:intergenic
GGTATTATTGGAACAAACGG+TGG 0.700731 3.1:-24692360 MS.gene000038:CDS
TTGGTGAAGTATGATGGCAA+TGG 0.739876 3.1:-24692330 MS.gene000038:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAAATATTATCTTCTATT+GGG + chr3.1:24692553-24692572 None:intergenic 15.0%
!! AGATAATATTTCATCACATT+TGG - chr3.1:24692559-24692578 MS.gene000038:CDS 20.0%
!! CATTTGCATTCATATAATAA+GGG + chr3.1:24692655-24692674 None:intergenic 20.0%
!! GATGAAATATTATCTTCTAT+TGG + chr3.1:24692554-24692573 None:intergenic 20.0%
!! TCATTTGCATTCATATAATA+AGG + chr3.1:24692656-24692675 None:intergenic 20.0%
!! TTCAAGATTAGAATAAGATT+CGG + chr3.1:24692324-24692343 None:intergenic 20.0%
! GAAATTAAAAGTTCATGTAG+AGG - chr3.1:24692341-24692360 MS.gene000038:CDS 25.0%
! TCAAGATTAGAATAAGATTC+GGG + chr3.1:24692323-24692342 None:intergenic 25.0%
! TTATCAATGGATGTTATTGT+TGG - chr3.1:24692737-24692756 MS.gene000038:CDS 25.0%
!! TTTTCGCAATGATTTATCAA+TGG - chr3.1:24692724-24692743 MS.gene000038:CDS 25.0%
AACCTATAGATTGTGATTTG+TGG - chr3.1:24692819-24692838 MS.gene000038:CDS 30.0%
AATCACAATCTATAGGTTCA+TGG + chr3.1:24692817-24692836 None:intergenic 30.0%
ACAAAAAGTGGTGGTATTAT+TGG - chr3.1:24692995-24693014 MS.gene000038:CDS 30.0%
AGGATGGAACAATTGAAATA+TGG - chr3.1:24692876-24692895 MS.gene000038:CDS 30.0%
ATCCACAAATCACAATCTAT+AGG + chr3.1:24692824-24692843 None:intergenic 30.0%
GGATGGAACAATTGAAATAT+GGG - chr3.1:24692877-24692896 MS.gene000038:CDS 30.0%
GTTCTTCAAGCATTTATCTA+TGG - chr3.1:24692381-24692400 MS.gene000038:CDS 30.0%
TATTGAGCATCTAAATTGGA+AGG + chr3.1:24692446-24692465 None:intergenic 30.0%
TCTTTAATGGGACTATTCAT+TGG - chr3.1:24692696-24692715 MS.gene000038:CDS 30.0%
TGGAAATGTCATCAATAGAT+AGG + chr3.1:24692949-24692968 None:intergenic 30.0%
TGGAGAATTTCTAAGTCTAT+GGG - chr3.1:24692847-24692866 MS.gene000038:CDS 30.0%
TGGATATTGAGCATCTAAAT+TGG + chr3.1:24692450-24692469 None:intergenic 30.0%
TTGGAGAATTTCTAAGTCTA+TGG - chr3.1:24692846-24692865 MS.gene000038:CDS 30.0%
! ACTTTTTGTAGAGCATATTG+GGG + chr3.1:24692984-24693003 None:intergenic 30.0%
! ATGATTACTTGGTGGTATTA+GGG - chr3.1:24692507-24692526 MS.gene000038:CDS 30.0%
! GATTGTGATTTGTGGATATT+TGG - chr3.1:24692827-24692846 MS.gene000038:CDS 30.0%
!! CACTTTTTGTAGAGCATATT+GGG + chr3.1:24692985-24693004 None:intergenic 30.0%
!! TATTGTTGGCTTTGATTTAG+TGG - chr3.1:24692751-24692770 MS.gene000038:CDS 30.0%
AAGCGATGCTTCAAAATCTA+TGG + chr3.1:24692255-24692274 None:intergenic 35.0%
AATACAAAGTGCATTCGTCT+TGG - chr3.1:24692909-24692928 MS.gene000038:CDS 35.0%
AGATTAGAATAAGATTCGGG+AGG + chr3.1:24692320-24692339 None:intergenic 35.0%
AGGTATTTGTTTGTGATGTC+CGG + chr3.1:24692417-24692436 None:intergenic 35.0%
ATACAAAGTGCATTCGTCTT+GGG - chr3.1:24692910-24692929 MS.gene000038:CDS 35.0%
ATATGCTCTACAAAAAGTGG+TGG - chr3.1:24692986-24693005 MS.gene000038:CDS 35.0%
CAAATGATGATCCCAAAGTA+GGG - chr3.1:24692669-24692688 MS.gene000038:CDS 35.0%
CAATATCCATGTTGTCTGTA+TGG - chr3.1:24692461-24692480 MS.gene000038:CDS 35.0%
CCAAGTAATCATCTCTTGAA+TGG + chr3.1:24692499-24692518 None:intergenic 35.0%
CCAATATGCTCTACAAAAAG+TGG - chr3.1:24692983-24693002 MS.gene000038:CDS 35.0%
CCATTCAAGAGATGATTACT+TGG - chr3.1:24692496-24692515 MS.gene000038:CDS 35.0%
GGTGGTATTATTGGAACAAA+CGG - chr3.1:24693004-24693023 MS.gene000038:CDS 35.0%
TATCTATGGAATCCATCAAC+CGG - chr3.1:24692395-24692414 MS.gene000038:CDS 35.0%
TCTAAATTGGAAGGAGGAAA+AGG + chr3.1:24692437-24692456 None:intergenic 35.0%
TGATTATACTTCACCAATCC+AGG + chr3.1:24692629-24692648 None:intergenic 35.0%
TTCAAGAGATGATTACTTGG+TGG - chr3.1:24692499-24692518 MS.gene000038:CDS 35.0%
TTTCATCGAGAGCTAATACA+TGG - chr3.1:24692588-24692607 MS.gene000038:CDS 35.0%
! ATTTCTAAGTCTATGGGCTA+TGG - chr3.1:24692853-24692872 MS.gene000038:CDS 35.0%
! CATGTTGTCTGTATGGTTTT+GGG - chr3.1:24692468-24692487 MS.gene000038:CDS 35.0%
! CTTTTTGTAGAGCATATTGG+GGG + chr3.1:24692983-24693002 None:intergenic 35.0%
! GATGATTACTTGGTGGTATT+AGG - chr3.1:24692506-24692525 MS.gene000038:CDS 35.0%
!! TTGGCTTTGATTTAGTGGAA+AGG - chr3.1:24692756-24692775 MS.gene000038:CDS 35.0%
!!! CCACTTTTTGTAGAGCATAT+TGG + chr3.1:24692986-24693005 None:intergenic 35.0%
AAAGTAGGGACGCTCTTTAA+TGG - chr3.1:24692683-24692702 MS.gene000038:CDS 40.0%
AAGTAGGGACGCTCTTTAAT+GGG - chr3.1:24692684-24692703 MS.gene000038:CDS 40.0%
ACTCTGTATACATAGCCACT+AGG + chr3.1:24693108-24693127 None:intergenic 40.0%
AGATATTGAGTGTCCTGGAT+TGG - chr3.1:24692613-24692632 MS.gene000038:CDS 40.0%
CCAAAACCATACAGACAACA+TGG + chr3.1:24692470-24692489 None:intergenic 40.0%
CTCTGTATACATAGCCACTA+GGG + chr3.1:24693107-24693126 None:intergenic 40.0%
GCAAATGATGATCCCAAAGT+AGG - chr3.1:24692668-24692687 MS.gene000038:CDS 40.0%
GGTATTATTGGAACAAACGG+TGG - chr3.1:24693007-24693026 MS.gene000038:CDS 40.0%
TATTGGGGGATAGTACTCAA+TGG + chr3.1:24692969-24692988 None:intergenic 40.0%
TCTGTATACATAGCCACTAG+GGG + chr3.1:24693106-24693125 None:intergenic 40.0%
TGAGCATCTAAATTGGAAGG+AGG + chr3.1:24692443-24692462 None:intergenic 40.0%
TGGAAAGATATTGAGTGTCC+TGG - chr3.1:24692608-24692627 MS.gene000038:CDS 40.0%
TGGGTCTTTACTCGTATCAT+AGG + chr3.1:24692534-24692553 None:intergenic 40.0%
TGGTCAAAACCATCTGGAAA+AGG + chr3.1:24692797-24692816 None:intergenic 40.0%
TTGGTGAAGTATGATGGCAA+TGG - chr3.1:24693037-24693056 MS.gene000038:CDS 40.0%
! ATTCGGGAGGCATAAAATCA+AGG + chr3.1:24692307-24692326 None:intergenic 40.0%
! CCATGTTGTCTGTATGGTTT+TGG - chr3.1:24692467-24692486 MS.gene000038:CDS 40.0%
! TAAATTTGCTCACTGTCACC+AGG + chr3.1:24693145-24693164 None:intergenic 40.0%
!! ACTGGATTGGTGAAGTATGA+TGG - chr3.1:24693031-24693050 MS.gene000038:CDS 40.0%
!! ATTAAAGAGCGTCCCTACTT+TGG + chr3.1:24692684-24692703 None:intergenic 40.0%
!! TTAAAGAGCGTCCCTACTTT+GGG + chr3.1:24692683-24692702 None:intergenic 40.0%
GGTTCATGGTCAAAACCATC+TGG + chr3.1:24692803-24692822 None:intergenic 45.0%
TGTTTGTGATGTCCGGTTGA+TGG + chr3.1:24692410-24692429 None:intergenic 45.0%
! TCTAAGTCTATGGGCTATGG+AGG - chr3.1:24692856-24692875 MS.gene000038:CDS 45.0%
! TTCGAGATGCCTTTTCCAGA+TGG - chr3.1:24692785-24692804 MS.gene000038:CDS 45.0%
ATAGCCACTAGGGGTTCACA+TGG + chr3.1:24693097-24693116 None:intergenic 50.0%
ATTGGAACAAACGGTGGCAC+TGG - chr3.1:24693013-24693032 MS.gene000038:CDS 50.0%
GAGTCTCTGCTTTCACTTCC+TGG - chr3.1:24693124-24693143 MS.gene000038:CDS 50.0%
GGGGTTCACATGGTTCGTTA+TGG + chr3.1:24693087-24693106 None:intergenic 50.0%
! AACAAACGGTGGCACTGGAT+TGG - chr3.1:24693018-24693037 MS.gene000038:CDS 50.0%
! AGTCTATGGGCTATGGAGGA+TGG - chr3.1:24692860-24692879 MS.gene000038:CDS 50.0%
CGAACCATGTGAACCCCTAG+TGG - chr3.1:24693090-24693109 MS.gene000038:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 24692224 24693165 24692224 ID=MS.gene000038
chr3.1 mRNA 24692224 24693165 24692224 ID=MS.gene000038.t1;Parent=MS.gene000038
chr3.1 exon 24692224 24693165 24692224 ID=MS.gene000038.t1.exon1;Parent=MS.gene000038.t1
chr3.1 CDS 24692224 24693165 24692224 ID=cds.MS.gene000038.t1;Parent=MS.gene000038.t1
Gene Sequence

>MS.gene000038

ATGTCAACTCTAGCTCTTGAAACTCGATCCATAGATTTTGAAGCATCGCTTAACGATGATTCTGCTTCTACTTCACTGAACCTTGATTTTATGCCTCCCGAATCTTATTCTAATCTTGAAATTAAAAGTTCATGTAGAGGATTTATAGTTTTGACTTGTTCTTCAAGCATTTATCTATGGAATCCATCAACCGGACATCACAAACAAATACCTTTTCCTCCTTCCAATTTAGATGCTCAATATCCATGTTGTCTGTATGGTTTTGGGTACGACCATTCAAGAGATGATTACTTGGTGGTATTAGGGTCCTATGATACGAGTAAAGACCCAATAGAAGATAATATTTCATCACATTTGGAGTTTTTTTCATCGAGAGCTAATACATGGAAAGATATTGAGTGTCCTGGATTGGTGAAGTATAATCACTTCCCTTATTATATGAATGCAAATGATGATCCCAAAGTAGGGACGCTCTTTAATGGGACTATTCATTGGTTCTCTTTTCGCAATGATTTATCAATGGATGTTATTGTTGGCTTTGATTTAGTGGAAAGGAAACTTTTCGAGATGCCTTTTCCAGATGGTTTTGACCATGAACCTATAGATTGTGATTTGTGGATATTTGGAGAATTTCTAAGTCTATGGGCTATGGAGGATGGAACAATTGAAATATGGGTTATGAAAGAATACAAAGTGCATTCGTCTTGGGTTAAAACTCTTGTCCTATCTATTGATGACATTTCCATTGAGTACTATCCCCCAATATGCTCTACAAAAAGTGGTGGTATTATTGGAACAAACGGTGGCACTGGATTGGTGAAGTATGATGGCAATGGTCAGTTGCTAGAGAATCGCTCATACCATAACGAACCATGTGAACCCCTAGTGGCTATGTATACAGAGTCTCTGCTTTCACTTCCTGGTGACAGTGAGCAAATTTAA
Protein sequence

>MS.gene000038.t1

MSTLALETRSIDFEASLNDDSASTSLNLDFMPPESYSNLEIKSSCRGFIVLTCSSSIYLWNPSTGHHKQIPFPPSNLDAQYPCCLYGFGYDHSRDDYLVVLGSYDTSKDPIEDNISSHLEFFSSRANTWKDIECPGLVKYNHFPYYMNANDDPKVGTLFNGTIHWFSFRNDLSMDVIVGFDLVERKLFEMPFPDGFDHEPIDCDLWIFGEFLSLWAMEDGTIEIWVMKEYKVHSSWVKTLVLSIDDISIEYYPPICSTKSGGIIGTNGGTGLVKYDGNGQLLENRSYHNEPCEPLVAMYTESLLSLPGDSEQI