AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58319


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58319.t1 MTR_8g433030 64.815 162 53 2 32 189 178 339 8.13e-64 200
MS.gene58319.t1 MTR_8g032390 64.815 162 53 2 32 189 211 372 2.98e-63 199
MS.gene58319.t1 MTR_8g433040 62.805 164 47 2 27 187 207 359 6.24e-60 191
MS.gene58319.t1 MTR_8g032380 62.805 164 47 2 27 187 207 359 6.24e-60 191
MS.gene58319.t1 MTR_6g060650 52.096 167 68 5 26 186 207 367 7.26e-45 152
MS.gene58319.t1 MTR_4g023130 54.088 159 65 4 34 187 153 308 9.43e-45 150
MS.gene58319.t1 MTR_4g023080 52.830 159 67 4 34 187 194 349 5.65e-44 149
MS.gene58319.t1 MTR_4g116320 50.909 165 74 3 29 187 90 253 5.34e-43 144
MS.gene58319.t1 MTR_4g116330 50.909 165 72 4 31 187 212 375 6.22e-43 147
MS.gene58319.t1 MTR_3g103020 50.909 165 74 3 29 187 210 373 1.31e-42 146
MS.gene58319.t1 MTR_8g040935 47.674 172 81 3 25 187 32 203 1.47e-42 142
MS.gene58319.t1 MTR_4g117720 50.877 171 71 4 29 188 205 373 2.33e-42 146
MS.gene58319.t1 MTR_6g463430 51.923 156 70 4 35 187 44 197 1.75e-41 139
MS.gene58319.t1 MTR_4g109270 48.521 169 78 4 25 187 194 359 2.03e-41 143
MS.gene58319.t1 MTR_4g094365 51.572 159 69 4 35 186 201 358 2.79e-41 142
MS.gene58319.t1 MTR_4g021785 51.299 154 69 3 38 187 199 350 7.23e-41 141
MS.gene58319.t1 MTR_1g055175 49.351 154 70 4 40 187 45 196 1.45e-40 136
MS.gene58319.t1 MTR_4g122850 55.346 159 62 6 36 187 203 359 2.34e-40 140
MS.gene58319.t1 MTR_4g023090 48.256 172 70 4 34 187 209 379 3.15e-40 140
MS.gene58319.t1 MTR_1g055165 49.351 154 70 4 40 187 162 313 3.90e-39 136
MS.gene58319.t1 MTR_4g118380 52.667 150 55 4 29 165 209 355 5.03e-39 137
MS.gene58319.t1 MTR_4g023380 45.251 179 71 4 34 186 194 371 1.08e-38 136
MS.gene58319.t1 MTR_4g109280 51.899 158 64 5 36 185 199 352 1.49e-38 135
MS.gene58319.t1 MTR_4g023070 46.857 175 73 4 30 187 194 365 3.42e-38 135
MS.gene58319.t1 MTR_4g117850 45.882 170 83 3 25 185 204 373 1.98e-37 133
MS.gene58319.t1 MTR_3g034130 52.857 140 59 4 29 164 210 346 2.14e-37 132
MS.gene58319.t1 MTR_5g097210 50.980 153 67 4 40 187 209 358 7.41e-37 131
MS.gene58319.t1 MTR_5g070080 50.625 160 71 4 35 188 224 381 7.58e-37 131
MS.gene58319.t1 MTR_4g023120 46.108 167 72 3 38 187 200 365 3.42e-36 129
MS.gene58319.t1 MTR_3g034180 48.485 165 75 5 29 187 324 484 1.70e-35 129
MS.gene58319.t1 MTR_1g055135 48.408 157 70 5 40 187 216 370 2.09e-35 127
MS.gene58319.t1 MTR_1g055195 49.677 155 65 6 40 186 323 472 1.09e-33 125
MS.gene58319.t1 MTR_4g023190 50.000 170 67 7 31 187 190 354 1.37e-33 122
MS.gene58319.t1 MTR_4g023150 48.851 174 70 7 28 187 187 355 3.59e-33 121
MS.gene58319.t1 MTR_5g070130 48.387 155 72 4 41 188 219 372 9.32e-33 120
MS.gene58319.t1 MTR_3g034160 49.390 164 75 4 29 187 210 370 1.23e-32 120
MS.gene58319.t1 MTR_4g023200 46.988 166 71 6 34 187 195 355 7.18e-32 118
MS.gene58319.t1 MTR_4g118400 46.154 169 60 6 29 186 146 294 9.96e-32 116
MS.gene58319.t1 MTR_4g117870 51.515 132 53 4 61 187 137 262 6.72e-31 113
MS.gene58319.t1 MTR_4g117780 43.373 166 84 5 31 187 198 362 1.82e-29 112
MS.gene58319.t1 MTR_7g079400 45.122 164 76 6 36 188 173 333 2.40e-29 110
MS.gene58319.t1 MTR_4g117770 45.342 161 78 5 36 187 203 362 8.38e-29 110
MS.gene58319.t1 MTR_4g117810 45.342 161 78 5 36 187 203 362 8.38e-29 110
MS.gene58319.t1 MTR_5g070088 44.767 172 84 5 27 188 211 381 1.43e-28 109
MS.gene58319.t1 MTR_7g079370 41.899 179 92 7 16 188 180 352 1.50e-28 109
MS.gene58319.t1 MTR_4g118370 44.371 151 60 4 41 187 125 255 4.14e-28 106
MS.gene58319.t1 MTR_1g055250 47.656 128 62 4 40 165 115 239 7.16e-28 107
MS.gene58319.t1 MTR_4g117530 40.764 157 57 4 35 187 124 248 4.11e-26 100
MS.gene58319.t1 MTR_4g118360 53.333 105 41 3 88 186 18 120 1.11e-25 96.3
MS.gene58319.t1 MTR_4g117750 52.830 106 44 2 88 187 373 478 1.91e-25 102
MS.gene58319.t1 MTR_4g117750 45.625 160 71 6 40 187 199 354 1.04e-23 97.4
MS.gene58319.t1 MTR_8g102520 38.462 169 94 5 27 188 153 318 9.26e-25 98.6
MS.gene58319.t1 MTR_7g079420 39.024 164 85 7 35 188 982 1140 1.64e-24 100
MS.gene58319.t1 MTR_4g122860 39.873 158 58 4 39 186 184 314 3.44e-24 97.4
MS.gene58319.t1 MTR_5g023000 56.989 93 34 2 101 187 2 94 4.08e-24 91.3
MS.gene58319.t1 MTR_4g121580 47.287 129 41 4 35 162 217 319 5.58e-24 97.4
MS.gene58319.t1 MTR_7g079450 43.704 135 67 4 59 188 3 133 1.63e-22 88.6
MS.gene58319.t1 MTR_8g098830 41.611 149 67 7 27 165 58 196 1.76e-22 90.9
MS.gene58319.t1 MTR_4g023440 39.873 158 53 4 34 187 208 327 1.42e-21 90.1
MS.gene58319.t1 MTR_4g116330 38.788 165 53 4 31 187 212 336 8.39e-21 88.2
MS.gene58319.t1 MTR_4g117430 58.065 62 25 1 36 97 92 152 1.77e-16 73.2
MS.gene58319.t1 MTR_2g048580 33.333 171 84 7 31 188 185 338 9.02e-16 74.3
MS.gene58319.t1 MTR_7g117720 45.977 87 37 3 111 187 186 272 3.96e-12 63.5
MS.gene58319.t1 MTR_4g114700 53.968 63 25 1 106 164 132 194 3.20e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGTTGTCTTTGATTTAA+TGG 0.084690 8.2:-65726575 MS.gene58319:CDS
TATTATGATTTGTGGGTATT+TGG 0.191816 8.2:-65726499 MS.gene58319:CDS
ATAATTGGACAATTGAAATA+TGG 0.213992 8.2:-65726444 MS.gene58319:CDS
TTGGAAAATTTCTCAGTCTA+TGG 0.237424 8.2:-65726480 MS.gene58319:CDS
TAATCCTGCTTTGGAAGCTT+TGG 0.326447 8.2:+65726657 None:intergenic
TAATCCTGCTTTGGAAGCTT+TGG 0.330098 8.2:+65726748 None:intergenic
TCTTCAATGGGGCTATTCAT+TGG 0.385937 8.2:-65726724 MS.gene58319:CDS
TGTTCAATGGGGCTATTCAT+TGG 0.388420 8.2:-65726633 MS.gene58319:CDS
ATTGAACAATAATCCTGCTT+TGG 0.394314 8.2:+65726648 None:intergenic
ATTGAAGAATAATCCTGCTT+TGG 0.397965 8.2:+65726739 None:intergenic
AAAGCAGGATTATTCTTCAA+TGG 0.398001 8.2:-65726737 MS.gene58319:CDS
TAATTGGACAATTGAAATAT+GGG 0.403350 8.2:-65726443 MS.gene58319:CDS
ATCTTGGGTATTATGATTTG+TGG 0.405974 8.2:-65726507 MS.gene58319:CDS
AAGCAGGATTATTGTTCAAT+GGG 0.407485 8.2:-65726645 MS.gene58319:CDS
AAGCAGGATTATTCTTCAAT+GGG 0.407987 8.2:-65726736 MS.gene58319:CDS
CAATGGGGCTATTCATTGGT+TGG 0.409278 8.2:-65726629 MS.gene58319:CDS
CAATGGGGCTATTCATTGGT+TGG 0.409603 8.2:-65726720 MS.gene58319:CDS
AAAGCAGGATTATTGTTCAA+TGG 0.422321 8.2:-65726646 MS.gene58319:CDS
ACTAAAAGTGGTGATATTGT+TGG 0.440141 8.2:-65726337 MS.gene58319:CDS
AAACGGAGACTCTGTATTCA+TGG 0.450730 8.2:+65726225 None:intergenic
AGATTGTGGTCAAAATCAAC+TGG 0.461270 8.2:+65726526 None:intergenic
TCTTGGGTATTATGATTTGT+GGG 0.466968 8.2:-65726506 MS.gene58319:CDS
TATGCCAAAGCTTCCAAAGC+AGG 0.478827 8.2:-65726661 MS.gene58319:CDS
TATGCCAAAGCTTCCAAAGC+AGG 0.478827 8.2:-65726752 MS.gene58319:CDS
CTTATATACTCTACTAAAAG+TGG 0.484913 8.2:-65726349 MS.gene58319:CDS
TTTGTGAAGTATAATGATAA+AGG 0.492510 8.2:-65726295 MS.gene58319:CDS
GGTCCACCAGGGAGTGAAAA+CGG 0.498553 8.2:+65726208 None:intergenic
AATACAAACAACATTCGTCT+TGG 0.503398 8.2:-65726411 MS.gene58319:CDS
GGAAGCTTTGGCATAATCCA+TGG 0.505733 8.2:+65726669 None:intergenic
TTGTCTTTGATTTAATGGAA+AGG 0.526787 8.2:-65726570 MS.gene58319:CDS
TGGTCAAAATCAACTGGAAG+AGG 0.526874 8.2:+65726532 None:intergenic
AAATTTGATCATGGTCCACC+AGG 0.534299 8.2:+65726196 None:intergenic
GGTGATATTGTTGGAACTGA+TGG 0.589833 8.2:-65726328 MS.gene58319:CDS
AATCATAATACCCAAGATTG+TGG 0.590736 8.2:+65726512 None:intergenic
ATATAAGGGAAAAGCACAGA+AGG 0.594922 8.2:+65726365 None:intergenic
CATGATATGCGTGAATACCA+TGG 0.597731 8.2:-65726686 MS.gene58319:CDS
GTTGGAACTGATGGTGCTAG+TGG 0.597956 8.2:-65726319 MS.gene58319:CDS
GAATCTGAATGCATCTGAAG+TGG 0.614691 8.2:-65726248 MS.gene58319:CDS
AATTTGATCATGGTCCACCA+GGG 0.660460 8.2:+65726197 None:intergenic
AGCAGGATTATTCTTCAATG+GGG 0.673360 8.2:-65726735 MS.gene58319:CDS
AGCAGGATTATTGTTCAATG+GGG 0.682100 8.2:-65726644 MS.gene58319:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTGGACAATTGAAATA+TGG - chr8.2:65726511-65726530 MS.gene58319:CDS 20.0%
!! TAATTGGACAATTGAAATAT+GGG - chr8.2:65726512-65726531 MS.gene58319:CDS 20.0%
!! TTTGTGAAGTATAATGATAA+AGG - chr8.2:65726660-65726679 MS.gene58319:CDS 20.0%
!!! ACTTTTAGTAGAGTATATAA+GGG + chr8.2:65726607-65726626 None:intergenic 20.0%
!!! GGAAAAGAAATTTTCATAAT+TGG - chr8.2:65726496-65726515 MS.gene58319:CDS 20.0%
!!! TATTGTTGTCTTTGATTTAA+TGG - chr8.2:65726380-65726399 MS.gene58319:CDS 20.0%
! CTTATATACTCTACTAAAAG+TGG - chr8.2:65726606-65726625 MS.gene58319:CDS 25.0%
!! CACTTTTAGTAGAGTATATA+AGG + chr8.2:65726608-65726627 None:intergenic 25.0%
!! TATTATGATTTGTGGGTATT+TGG - chr8.2:65726456-65726475 MS.gene58319:CDS 25.0%
!! TTGTCTTTGATTTAATGGAA+AGG - chr8.2:65726385-65726404 MS.gene58319:CDS 25.0%
AAAGCAGGATTATTCTTCAA+TGG - chr8.2:65726218-65726237 MS.gene58319:CDS 30.0%
AAAGCAGGATTATTGTTCAA+TGG - chr8.2:65726309-65726328 MS.gene58319:CDS 30.0%
AAGCAGGATTATTCTTCAAT+GGG - chr8.2:65726219-65726238 MS.gene58319:CDS 30.0%
AAGCAGGATTATTGTTCAAT+GGG - chr8.2:65726310-65726329 MS.gene58319:CDS 30.0%
AATACAAACAACATTCGTCT+TGG - chr8.2:65726544-65726563 MS.gene58319:CDS 30.0%
AATCATAATACCCAAGATTG+TGG + chr8.2:65726446-65726465 None:intergenic 30.0%
ACTAAAAGTGGTGATATTGT+TGG - chr8.2:65726618-65726637 MS.gene58319:CDS 30.0%
ATCTTGGGTATTATGATTTG+TGG - chr8.2:65726448-65726467 MS.gene58319:CDS 30.0%
ATTGAACAATAATCCTGCTT+TGG + chr8.2:65726310-65726329 None:intergenic 30.0%
ATTGAAGAATAATCCTGCTT+TGG + chr8.2:65726219-65726238 None:intergenic 30.0%
TTGGAAAATTTCTCAGTCTA+TGG - chr8.2:65726475-65726494 MS.gene58319:CDS 30.0%
! TCTTGGGTATTATGATTTGT+GGG - chr8.2:65726449-65726468 MS.gene58319:CDS 30.0%
!! TTGATTTTGACCACAATCTT+GGG - chr8.2:65726433-65726452 MS.gene58319:CDS 30.0%
AGATTGTGGTCAAAATCAAC+TGG + chr8.2:65726432-65726451 None:intergenic 35.0%
AGCAGGATTATTCTTCAATG+GGG - chr8.2:65726220-65726239 MS.gene58319:CDS 35.0%
AGCAGGATTATTGTTCAATG+GGG - chr8.2:65726311-65726330 MS.gene58319:CDS 35.0%
! ATATAAGGGAAAAGCACAGA+AGG + chr8.2:65726593-65726612 None:intergenic 35.0%
!! GTTGATTTTGACCACAATCT+TGG - chr8.2:65726432-65726451 MS.gene58319:CDS 35.0%
AAACGGAGACTCTGTATTCA+TGG + chr8.2:65726733-65726752 None:intergenic 40.0%
CATGATATGCGTGAATACCA+TGG - chr8.2:65726269-65726288 MS.gene58319:CDS 40.0%
GAATCTGAATGCATCTGAAG+TGG - chr8.2:65726707-65726726 MS.gene58319:CDS 40.0%
GGTGATATTGTTGGAACTGA+TGG - chr8.2:65726627-65726646 MS.gene58319:CDS 40.0%
TCTTCAATGGGGCTATTCAT+TGG - chr8.2:65726231-65726250 MS.gene58319:CDS 40.0%
TGGTCAAAATCAACTGGAAG+AGG + chr8.2:65726426-65726445 None:intergenic 40.0%
TGTTCAATGGGGCTATTCAT+TGG - chr8.2:65726322-65726341 MS.gene58319:CDS 40.0%
!! TAATCCTGCTTTGGAAGCTT+TGG + chr8.2:65726210-65726229 None:intergenic 40.0%
CAATGGGGCTATTCATTGGT+TGG - chr8.2:65726235-65726254 MS.gene58319:CDS 45.0%
TATGCCAAAGCTTCCAAAGC+AGG - chr8.2:65726294-65726313 MS.gene58319:CDS 45.0%
! GGAAGCTTTGGCATAATCCA+TGG + chr8.2:65726289-65726308 None:intergenic 45.0%
! GTTGGAACTGATGGTGCTAG+TGG - chr8.2:65726636-65726655 MS.gene58319:CDS 50.0%
GGTCCACCAGGGAGTGAAAA+CGG + chr8.2:65726750-65726769 None:intergenic 55.0%
! GAGTCTCCGTTTTCACTCCC+TGG - chr8.2:65726741-65726760 MS.gene58319:CDS 55.0%
! TCTCCGTTTTCACTCCCTGG+TGG - chr8.2:65726744-65726763 MS.gene58319:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 65726204 65726773 65726204 ID=MS.gene58319
chr8.2 mRNA 65726204 65726773 65726204 ID=MS.gene58319.t1;Parent=MS.gene58319
chr8.2 exon 65726204 65726773 65726204 ID=MS.gene58319.t1.exon1;Parent=MS.gene58319.t1
chr8.2 CDS 65726204 65726773 65726204 ID=cds.MS.gene58319.t1;Parent=MS.gene58319.t1
Gene Sequence

>MS.gene58319

ATGCCAAAGCTTCCAAAGCAGGATTATTCTTCAATGGGGCTATTCATTGGTTGGTTTCTCATTATCATGATATGCGTGAATACCATGGATTATGCCAAAGCTTCCAAAGCAGGATTATTGTTCAATGGGGCTATTCATTGGTTGGCTTCTCATTATCATGATATGCGAGAGAAAGTTATTGTTGTCTTTGATTTAATGGAAAGGAAACTGTTACATATACCTCTTCCAGTTGATTTTGACCACAATCTTGGGTATTATGATTTGTGGGTATTTGGAAAATTTCTCAGTCTATGGAAAAGAAATTTTCATAATTGGACAATTGAAATATGGGTGATGAAGAAATACAAACAACATTCGTCTTGGACTAAGTCTCTTGTAATTTCTAGCCTTCTGTGCTTTTCCCTTATATACTCTACTAAAAGTGGTGATATTGTTGGAACTGATGGTGCTAGTGGATTTGTGAAGTATAATGATAAAGGACAGCAGATAGAGTATCTCGAAATGAATCTGAATGCATCTGAAGTGGCCATGAATACAGAGTCTCCGTTTTCACTCCCTGGTGGACCATGA

Protein sequence

>MS.gene58319.t1

MPKLPKQDYSSMGLFIGWFLIIMICVNTMDYAKASKAGLLFNGAIHWLASHYHDMREKVIVVFDLMERKLLHIPLPVDFDHNLGYYDLWVFGKFLSLWKRNFHNWTIEIWVMKKYKQHSSWTKSLVISSLLCFSLIYSTKSGDIVGTDGASGFVKYNDKGQQIEYLEMNLNASEVAMNTESPFSLPGGP