AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene045476

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045476.t1 MTR_4g080200 55.056 267 101 8 1 256 1 259 9.57e-86 261
MS.gene045476.t1 MTR_1g009110 46.953 279 120 12 2 256 13 287 8.50e-62 200
MS.gene045476.t1 MTR_1g023400 43.299 291 133 13 1 271 1 279 1.62e-59 193
MS.gene045476.t1 MTR_1g022200 43.015 272 136 9 1 256 13 281 3.42e-53 178
MS.gene045476.t1 MTR_1g037540 40.391 307 124 13 3 256 5 305 2.02e-49 169
MS.gene045476.t1 MTR_1g022105 35.165 273 116 9 2 256 20 249 2.12e-33 124
MS.gene045476.t1 MTR_8g093280 62.626 99 34 2 7 104 11 107 5.27e-31 112
MS.gene045476.t1 MTR_3g014540 27.419 248 159 7 2 234 28 269 1.91e-18 84.7
MS.gene045476.t1 MTR_2g021150 25.000 268 175 7 3 252 14 273 2.40e-18 84.3
MS.gene045476.t1 MTR_3g007410 28.629 248 150 10 6 234 55 294 4.53e-18 84.0
MS.gene045476.t1 MTR_2g034700 28.405 257 133 13 6 227 17 257 5.76e-18 83.2
MS.gene045476.t1 MTR_3g007360 28.226 248 151 10 6 234 55 294 6.45e-18 83.6
MS.gene045476.t1 MTR_6g007135 27.092 251 159 7 3 234 26 271 1.13e-17 82.8
MS.gene045476.t1 MTR_4g133170 24.701 251 161 7 2 235 3 242 2.55e-17 81.6
MS.gene045476.t1 MTR_3g103020 25.448 279 184 8 3 266 20 289 4.83e-17 80.9
MS.gene045476.t1 MTR_4g116330 28.185 259 157 11 3 245 23 268 7.82e-17 80.1
MS.gene045476.t1 MTR_8g063370 27.698 278 158 12 7 253 28 293 9.85e-17 80.1
MS.gene045476.t1 MTR_7g070440 28.514 249 151 9 5 234 9 249 1.84e-16 79.0
MS.gene045476.t1 MTR_7g016930 27.798 277 153 12 6 253 19 277 2.17e-16 78.6
MS.gene045476.t1 MTR_0667s0010 28.428 299 179 14 3 274 6 296 2.68e-16 79.0
MS.gene045476.t1 MTR_8g013010 27.477 222 138 6 1 206 52 266 4.10e-16 77.4
MS.gene045476.t1 MTR_3g014570 27.490 251 155 9 2 234 28 269 5.49e-16 77.8
MS.gene045476.t1 MTR_3g007270 26.820 261 154 9 3 234 47 299 6.23e-16 77.8
MS.gene045476.t1 MTR_3g013770 27.059 255 154 7 3 234 33 278 9.36e-16 77.0
MS.gene045476.t1 MTR_2g028140 25.796 314 175 13 3 271 5 305 1.22e-15 77.0
MS.gene045476.t1 MTR_4g023090 28.682 258 145 12 3 238 16 256 1.36e-15 76.6
MS.gene045476.t1 MTR_4g117850 27.132 258 160 12 2 243 22 267 2.26e-15 75.9
MS.gene045476.t1 MTR_4g116340 27.907 215 128 6 3 206 36 234 3.90e-15 73.6
MS.gene045476.t1 MTR_6g053260 28.346 254 148 12 2 234 336 576 5.15e-15 75.5
MS.gene045476.t1 MTR_4g028510 24.727 275 157 10 3 234 16 283 8.09e-15 74.7
MS.gene045476.t1 MTR_4g094365 27.626 257 153 10 3 240 8 250 8.32e-15 74.3
MS.gene045476.t1 MTR_8g073550 31.746 189 108 6 3 176 30 212 9.54e-15 72.4
MS.gene045476.t1 MTR_5g092850 25.620 242 131 9 6 206 19 252 9.66e-15 74.3
MS.gene045476.t1 MTR_3g034130 26.545 275 159 10 1 245 7 268 1.28e-14 73.6
MS.gene045476.t1 MTR_8g046310 28.782 271 125 14 6 235 19 262 1.60e-14 73.6
MS.gene045476.t1 MTR_3g007330 23.485 264 172 8 2 247 47 298 1.70e-14 73.6
MS.gene045476.t1 MTR_2g046920 26.452 310 184 12 3 289 24 312 1.74e-14 73.2
MS.gene045476.t1 MTR_4g116330 30.594 219 124 10 3 205 23 229 2.30e-14 72.8
MS.gene045476.t1 MTR_3g007430 27.126 247 156 7 6 235 46 285 2.66e-14 72.8
MS.gene045476.t1 MTR_5g097220 26.599 297 139 15 2 234 12 293 4.40e-14 72.4
MS.gene045476.t1 MTR_7g013590 27.984 243 155 9 6 234 39 275 5.62e-14 72.0
MS.gene045476.t1 MTR_2g028130 25.468 267 157 9 3 235 5 263 5.87e-14 72.0
MS.gene045476.t1 MTR_5g074850 27.037 270 140 12 6 227 38 298 7.30e-14 72.0
MS.gene045476.t1 MTR_4g061340 24.907 269 144 10 2 234 25 271 7.51e-14 71.2
MS.gene045476.t1 MTR_6g080680 27.376 263 151 11 6 235 22 277 7.70e-14 71.6
MS.gene045476.t1 MTR_3g030350 24.303 251 155 10 2 234 34 267 7.75e-14 71.6
MS.gene045476.t1 MTR_1g007430 29.353 201 100 8 14 176 7 203 8.65e-14 70.9
MS.gene045476.t1 MTR_4g023440 30.455 220 123 8 3 206 18 223 9.56e-14 70.9
MS.gene045476.t1 MTR_4g028660 23.954 263 151 11 6 227 19 273 1.01e-13 71.2
MS.gene045476.t1 MTR_4g027450 23.954 263 151 11 6 227 19 273 1.01e-13 71.2
MS.gene045476.t1 MTR_4g023080 28.916 249 141 13 3 235 9 237 1.07e-13 70.9
MS.gene045476.t1 MTR_2g048580 25.769 260 155 11 10 253 15 252 1.59e-13 70.1
MS.gene045476.t1 MTR_4g108820 28.571 245 119 13 3 234 42 243 2.12e-13 69.7
MS.gene045476.t1 MTR_5g027330 25.806 310 179 12 2 274 39 334 2.83e-13 70.1
MS.gene045476.t1 MTR_2g065860 26.667 240 130 9 6 208 15 245 3.07e-13 69.7
MS.gene045476.t1 MTR_3g013740 24.621 264 159 10 2 234 43 297 3.46e-13 69.7
MS.gene045476.t1 MTR_5g075370 26.374 273 141 12 6 227 11 274 4.37e-13 69.7
MS.gene045476.t1 MTR_5g027370 26.198 313 174 13 2 274 28 323 8.57e-13 68.6
MS.gene045476.t1 MTR_3g009310 25.781 256 148 9 3 233 42 280 9.35e-13 68.2
MS.gene045476.t1 MTR_1g094210 24.658 292 170 12 3 276 57 316 1.07e-12 68.2
MS.gene045476.t1 MTR_1g094210 24.658 292 170 12 3 276 57 316 1.14e-12 68.2
MS.gene045476.t1 MTR_3g011120 27.797 295 154 16 6 262 17 290 1.24e-12 67.8
MS.gene045476.t1 MTR_7g066840 30.075 266 135 13 3 233 23 272 1.35e-12 67.8
MS.gene045476.t1 MTR_7g066850 31.308 214 120 8 3 202 23 223 1.60e-12 67.8
MS.gene045476.t1 MTR_8g464770 27.586 261 143 10 6 233 15 262 1.73e-12 67.4
MS.gene045476.t1 MTR_7g017040 24.534 322 182 17 6 277 19 329 1.76e-12 67.4
MS.gene045476.t1 MTR_4g023120 30.317 221 129 10 1 206 1 211 1.89e-12 67.4
MS.gene045476.t1 MTR_0242s0060 27.027 296 158 14 3 252 9 292 1.92e-12 67.4
MS.gene045476.t1 MTR_0242s0040 27.027 296 158 14 3 252 9 292 1.92e-12 67.4
MS.gene045476.t1 MTR_2g021950 26.007 273 152 11 2 233 15 278 2.30e-12 67.0
MS.gene045476.t1 MTR_1g097330 28.163 245 149 10 3 234 16 246 2.33e-12 67.0
MS.gene045476.t1 MTR_1g055070 25.373 268 154 10 2 234 48 304 2.48e-12 67.0
MS.gene045476.t1 MTR_6g060650 24.042 287 184 8 17 278 22 299 2.65e-12 67.0
MS.gene045476.t1 MTR_3g011060 22.180 266 160 7 2 234 27 278 3.46e-12 66.6
MS.gene045476.t1 MTR_8g032390 25.301 249 151 9 3 241 40 263 3.83e-12 66.2
MS.gene045476.t1 MTR_5g096900 26.693 251 140 9 6 221 15 256 3.90e-12 65.9
MS.gene045476.t1 MTR_4g109270 25.614 285 178 10 1 266 6 275 4.74e-12 65.9
MS.gene045476.t1 MTR_2g028160 25.279 269 155 9 3 234 5 264 5.36e-12 66.2
MS.gene045476.t1 MTR_4g117770 29.956 227 117 9 2 206 10 216 5.62e-12 65.9
MS.gene045476.t1 MTR_4g117810 29.956 227 117 9 2 206 10 216 5.62e-12 65.9
MS.gene045476.t1 MTR_2g066170 27.778 252 134 12 6 221 15 254 6.52e-12 65.9
MS.gene045476.t1 MTR_8g063440 27.542 236 123 11 6 204 15 239 6.58e-12 65.9
MS.gene045476.t1 MTR_5g011200 25.591 254 149 13 6 234 20 258 6.64e-12 65.5
MS.gene045476.t1 MTR_4g109280 24.498 249 168 8 6 244 11 249 7.62e-12 65.5
MS.gene045476.t1 MTR_1g054725 26.562 256 147 10 6 234 23 264 7.87e-12 65.5
MS.gene045476.t1 MTR_5g024260 30.924 249 135 14 6 225 25 265 8.00e-12 65.5
MS.gene045476.t1 MTR_4g070550 25.461 271 152 10 3 235 5 263 1.08e-11 65.1
MS.gene045476.t1 MTR_3g007300 30.288 208 118 7 2 205 48 232 1.10e-11 65.1
MS.gene045476.t1 MTR_4g028680 24.436 266 153 10 3 227 16 274 1.14e-11 65.1
MS.gene045476.t1 MTR_5g096910 26.766 269 150 10 3 234 17 275 1.19e-11 65.1
MS.gene045476.t1 MTR_3g034180 25.556 270 164 9 3 245 123 382 1.25e-11 65.1
MS.gene045476.t1 MTR_8g012090 27.468 233 115 11 6 210 19 225 1.30e-11 64.7
MS.gene045476.t1 MTR_6g004660 24.409 254 166 6 3 234 16 265 1.36e-11 64.7
MS.gene045476.t1 MTR_1g050542 27.381 252 161 11 3 246 58 295 1.50e-11 64.7
MS.gene045476.t1 MTR_8g101580 24.221 289 170 12 10 276 53 314 2.19e-11 64.3
MS.gene045476.t1 MTR_6g461660 23.361 244 141 7 3 205 19 257 2.19e-11 64.3
MS.gene045476.t1 MTR_7g066100 25.279 269 166 8 6 240 14 281 2.59e-11 63.9
MS.gene045476.t1 MTR_2g426070 25.887 282 167 11 6 252 9 283 2.91e-11 63.9
MS.gene045476.t1 MTR_4g023070 27.490 251 154 12 1 235 1 239 3.25e-11 63.5
MS.gene045476.t1 MTR_5g022420 27.381 252 151 11 3 241 27 259 3.32e-11 63.5
MS.gene045476.t1 MTR_6g017270 27.885 208 124 6 3 188 9 212 3.77e-11 63.2
MS.gene045476.t1 MTR_4g030760 30.256 195 109 7 6 183 76 260 4.32e-11 63.5
MS.gene045476.t1 MTR_2g426020 25.283 265 151 9 3 233 6 257 5.23e-11 63.2
MS.gene045476.t1 MTR_6g027910 27.239 268 150 14 2 234 8 265 5.27e-11 62.8
MS.gene045476.t1 MTR_2g021155 26.587 252 158 8 2 237 35 275 5.39e-11 63.5
MS.gene045476.t1 MTR_4g024890 29.032 186 115 6 6 178 13 194 5.40e-11 62.8
MS.gene045476.t1 MTR_6g065980 25.000 268 150 12 2 247 15 253 5.60e-11 62.8
MS.gene045476.t1 MTR_6g090010 64.286 42 15 0 2 43 54 95 5.61e-11 60.5
MS.gene045476.t1 MTR_5g070130 25.694 288 179 9 3 266 11 287 6.23e-11 62.8
MS.gene045476.t1 MTR_4g107820 25.758 264 140 11 2 236 35 271 7.24e-11 62.8
MS.gene045476.t1 MTR_4g107820 25.758 264 140 11 2 236 35 271 7.57e-11 62.4
MS.gene045476.t1 MTR_4g107780 25.758 264 140 11 2 236 35 271 7.57e-11 62.4
MS.gene045476.t1 MTR_4g107780 25.758 264 140 11 2 236 35 271 7.58e-11 62.8
MS.gene045476.t1 MTR_8g433040 28.704 216 122 8 6 201 21 224 7.96e-11 62.4
MS.gene045476.t1 MTR_8g032380 28.704 216 122 8 6 201 21 224 7.96e-11 62.4
MS.gene045476.t1 MTR_2g066150 27.647 170 97 6 1 151 10 172 8.03e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045476.t1 AT3G16210 23.387 248 161 9 3 242 5 231 1.34e-12 67.8
MS.gene045476.t1 AT2G40925 26.259 278 155 11 6 253 30 287 1.07e-11 65.1
MS.gene045476.t1 AT3G07870 23.899 318 193 13 2 285 27 329 1.80e-11 64.7
MS.gene045476.t1 AT5G62510 27.273 264 163 10 6 250 47 300 3.15e-11 63.9
MS.gene045476.t1 AT3G23260 22.568 257 177 8 2 247 5 250 7.42e-11 62.4
MS.gene045476.t1 AT1G12170 28.177 181 120 4 3 176 6 183 7.75e-11 62.4
MS.gene045476.t1 AT3G23880 24.615 260 147 9 2 233 13 251 8.11e-11 62.4

Find 55 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAATTCTTTCTATGTATT+TGG 0.132248 4.2:-29818502 None:intergenic
AGATATAATGCTCTTGTTAA+TGG 0.160555 4.2:+29818837 MS.gene045476:CDS
TAGAGAAGATACAATGAATT+TGG 0.191531 4.2:+29818248 None:intergenic
CTCATTAGTTGTTGGGTTCC+AGG 0.262133 4.2:-29818601 None:intergenic
ACATGAGAGAAGTTTCAATT+TGG 0.270140 4.2:-29818439 None:intergenic
AATGAATTTGGGAGCAGATT+TGG 0.275076 4.2:+29818260 None:intergenic
AAGGGTTTCTTGTATGGTTT+TGG 0.278467 4.2:+29818660 MS.gene045476:CDS
CTACAATTGTCCTCTTACTA+TGG 0.290006 4.2:+29818794 MS.gene045476:CDS
TGTGATGTCATCAAGATAAA+AGG 0.312539 4.2:+29818987 MS.gene045476:CDS
TGCGGTAAGGGTTTCTTGTA+TGG 0.314365 4.2:+29818654 MS.gene045476:CDS
ATATGTGGTTGCTGGATATA+TGG 0.322647 4.2:+29819056 MS.gene045476:CDS
TTGTCGTGCCAAGTTTCGTC+TGG 0.334654 4.2:-29818570 None:intergenic
TTGTTAATGGTGTTTATCAT+TGG 0.359584 4.2:+29818850 MS.gene045476:CDS
AAATTAAAGCAGAGTATGTT+TGG 0.374287 4.2:-29818903 None:intergenic
GATGCATCTCATTAGTTGTT+GGG 0.377980 4.2:-29818608 None:intergenic
CATCTTCTATTCACAAAGTA+AGG 0.385800 4.2:-29818369 None:intergenic
TTTCAATGACTAGAAGAATA+GGG 0.388602 4.2:-29818413 None:intergenic
GGCATACTGTGATGTGAGTT+TGG 0.392314 4.2:-29818957 None:intergenic
AGAGAAGATACAATGAATTT+GGG 0.403841 4.2:+29818249 None:intergenic
ATATGATAAATCAACATATC+TGG 0.408133 4.2:-29819121 None:intergenic
GTTGCTGGATATATGGGTTA+TGG 0.419237 4.2:+29819063 MS.gene045476:CDS
TCTTTCTATGTATTTGGTAA+AGG 0.419673 4.2:-29818496 None:intergenic
CTGTTTGTATATGTGGTTGC+TGG 0.421883 4.2:+29819048 MS.gene045476:CDS
ATCAAAACTGTTTGTATATG+TGG 0.425769 4.2:+29819041 MS.gene045476:CDS
ACAAGAGCATTATATCTTGA+AGG 0.432503 4.2:-29818831 None:intergenic
GGCACGACAAATTGATTGCC+TGG 0.443235 4.2:+29818583 MS.gene045476:CDS
CAAGAAACCCTTACCGCAAA+AGG 0.446133 4.2:-29818649 None:intergenic
GTAAAGGACTTAATGAGGAT+TGG 0.451064 4.2:-29818480 None:intergenic
TTATTTACCCAGACGAAACT+TGG 0.463089 4.2:+29818562 MS.gene045476:CDS
TGATGCATCTCATTAGTTGT+TGG 0.474994 4.2:-29818609 None:intergenic
TATGGGTTATGGATCAAAAC+AGG 0.476151 4.2:+29819074 MS.gene045476:CDS
ATAATCTAAGCACTAAATCC+TGG 0.483289 4.2:+29818763 MS.gene045476:CDS
TTTGGGAGCAGATTTGGAGA+TGG 0.495294 4.2:+29818266 MS.gene045476:CDS
GGGAGCAGATTTGGAGATGG+AGG 0.495402 4.2:+29818269 MS.gene045476:CDS
ATCAAGATAAAAGGTTCCCT+TGG 0.506414 4.2:+29818996 MS.gene045476:CDS
CTTGATGACATCACAGGAAG+AGG 0.520514 4.2:-29818979 None:intergenic
ATTTGGTAAAGGACTTAATG+AGG 0.524164 4.2:-29818485 None:intergenic
TATGTGGTTGCTGGATATAT+GGG 0.532537 4.2:+29819057 MS.gene045476:CDS
TGATGTGGGACCATAGTAAG+AGG 0.535665 4.2:-29818804 None:intergenic
TATCTTGAAGGACGTTGATG+TGG 0.544261 4.2:-29818819 None:intergenic
ATAGAAAGAATTGAATCTTA+CGG 0.546959 4.2:+29818510 MS.gene045476:CDS
TGTCGTGCCAAGTTTCGTCT+GGG 0.558835 4.2:-29818569 None:intergenic
GAATCTTACGGTAGTTGCAA+CGG 0.580081 4.2:+29818522 MS.gene045476:CDS
ATCTTGAAGGACGTTGATGT+GGG 0.583095 4.2:-29818818 None:intergenic
TTGAAACTTCTCTCATGTGA+CGG 0.592666 4.2:+29818444 MS.gene045476:CDS
AGATTATAAACTTCAACCAA+AGG 0.599741 4.2:-29818747 None:intergenic
TACAAGTAAAATCATTGATG+AGG 0.601362 4.2:+29818304 MS.gene045476:CDS
GAAACCCTTACCGCAAAAGG+AGG 0.607631 4.2:-29818646 None:intergenic
TTGATGACATCACAGGAAGA+GGG 0.615702 4.2:-29818978 None:intergenic
CCGCAAAAGGAGGGAGCACG+AGG 0.618495 4.2:-29818636 None:intergenic
TGGAGATGGAGGTGTTGTTG+CGG 0.620474 4.2:+29818280 MS.gene045476:CDS
AAACCCTTACCGCAAAAGGA+GGG 0.634888 4.2:-29818645 None:intergenic
GGGTTATGGATCAAAACAGG+TGG 0.656850 4.2:+29819077 MS.gene045476:CDS
ATAGCATGATAGCATAACCA+AGG 0.674219 4.2:-29819013 None:intergenic
TAGCATGATAGCATAACCAA+GGG 0.676481 4.2:-29819012 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGAAAGAATTGAATCTTA+CGG + chr4.2:29818510-29818529 MS.gene045476:CDS 20.0%
!! TTCAATTCTTTCTATGTATT+TGG - chr4.2:29818505-29818524 None:intergenic 20.0%
! AAATTAAAGCAGAGTATGTT+TGG - chr4.2:29818906-29818925 None:intergenic 25.0%
! AGATATAATGCTCTTGTTAA+TGG + chr4.2:29818837-29818856 MS.gene045476:CDS 25.0%
! AGATTATAAACTTCAACCAA+AGG - chr4.2:29818750-29818769 None:intergenic 25.0%
! ATCAAAACTGTTTGTATATG+TGG + chr4.2:29819041-29819060 MS.gene045476:CDS 25.0%
! TACAAGTAAAATCATTGATG+AGG + chr4.2:29818304-29818323 MS.gene045476:CDS 25.0%
! TCAAATGTGTTCAAAAATCA+TGG + chr4.2:29818328-29818347 MS.gene045476:CDS 25.0%
! TCTTTCTATGTATTTGGTAA+AGG - chr4.2:29818499-29818518 None:intergenic 25.0%
! TTGTTAATGGTGTTTATCAT+TGG + chr4.2:29818850-29818869 MS.gene045476:CDS 25.0%
! TTTCAATGACTAGAAGAATA+GGG - chr4.2:29818416-29818435 None:intergenic 25.0%
!! ACAATTGTAGATAATTTTCC+AGG - chr4.2:29818784-29818803 None:intergenic 25.0%
!! TTTTCAATGACTAGAAGAAT+AGG - chr4.2:29818417-29818436 None:intergenic 25.0%
ACATGAGAGAAGTTTCAATT+TGG - chr4.2:29818442-29818461 None:intergenic 30.0%
ATAATCTAAGCACTAAATCC+TGG + chr4.2:29818763-29818782 MS.gene045476:CDS 30.0%
ATTTGGTAAAGGACTTAATG+AGG - chr4.2:29818488-29818507 None:intergenic 30.0%
CATCTTCTATTCACAAAGTA+AGG - chr4.2:29818372-29818391 None:intergenic 30.0%
GACAAAAATGCTTTATCCTT+TGG + chr4.2:29818731-29818750 MS.gene045476:CDS 30.0%
TGTGATGTCATCAAGATAAA+AGG + chr4.2:29818987-29819006 MS.gene045476:CDS 30.0%
!! ACAAGAGCATTATATCTTGA+AGG - chr4.2:29818834-29818853 None:intergenic 30.0%
!! TTTTATCTTGATGACATCAC+AGG - chr4.2:29818988-29819007 None:intergenic 30.0%
ATAGCATGATAGCATAACCA+AGG - chr4.2:29819016-29819035 None:intergenic 35.0%
ATATGTGGTTGCTGGATATA+TGG + chr4.2:29819056-29819075 MS.gene045476:CDS 35.0%
ATCAAGATAAAAGGTTCCCT+TGG + chr4.2:29818996-29819015 MS.gene045476:CDS 35.0%
CTACAATTGTCCTCTTACTA+TGG + chr4.2:29818794-29818813 MS.gene045476:CDS 35.0%
GATGCATCTCATTAGTTGTT+GGG - chr4.2:29818611-29818630 None:intergenic 35.0%
GTAAAGGACTTAATGAGGAT+TGG - chr4.2:29818483-29818502 None:intergenic 35.0%
TAGCATGATAGCATAACCAA+GGG - chr4.2:29819015-29819034 None:intergenic 35.0%
TATGTGGTTGCTGGATATAT+GGG + chr4.2:29819057-29819076 MS.gene045476:CDS 35.0%
TGATGCATCTCATTAGTTGT+TGG - chr4.2:29818612-29818631 None:intergenic 35.0%
TTATTTACCCAGACGAAACT+TGG + chr4.2:29818562-29818581 MS.gene045476:CDS 35.0%
TTGAAACTTCTCTCATGTGA+CGG + chr4.2:29818444-29818463 MS.gene045476:CDS 35.0%
! AAGGGTTTCTTGTATGGTTT+TGG + chr4.2:29818660-29818679 MS.gene045476:CDS 35.0%
! ACTAGAAGAATAGGGTTTTC+AGG - chr4.2:29818408-29818427 None:intergenic 35.0%
! CTAGAAGAATAGGGTTTTCA+GGG - chr4.2:29818407-29818426 None:intergenic 35.0%
! TATGGGTTATGGATCAAAAC+AGG + chr4.2:29819074-29819093 MS.gene045476:CDS 35.0%
!! ACAGGTGGACTAAAAAGTAT+AGG + chr4.2:29819092-29819111 MS.gene045476:CDS 35.0%
ATCTTGAAGGACGTTGATGT+GGG - chr4.2:29818821-29818840 None:intergenic 40.0%
CTGTTTGTATATGTGGTTGC+TGG + chr4.2:29819048-29819067 MS.gene045476:CDS 40.0%
GAATCTTACGGTAGTTGCAA+CGG + chr4.2:29818522-29818541 MS.gene045476:CDS 40.0%
TATCTTGAAGGACGTTGATG+TGG - chr4.2:29818822-29818841 None:intergenic 40.0%
! GTTGCTGGATATATGGGTTA+TGG + chr4.2:29819063-29819082 MS.gene045476:CDS 40.0%
! TTGATGACATCACAGGAAGA+GGG - chr4.2:29818981-29819000 None:intergenic 40.0%
AAACCCTTACCGCAAAAGGA+GGG - chr4.2:29818648-29818667 None:intergenic 45.0%
CAAGAAACCCTTACCGCAAA+AGG - chr4.2:29818652-29818671 None:intergenic 45.0%
CTCATTAGTTGTTGGGTTCC+AGG - chr4.2:29818604-29818623 None:intergenic 45.0%
GGCATACTGTGATGTGAGTT+TGG - chr4.2:29818960-29818979 None:intergenic 45.0%
GGGTTATGGATCAAAACAGG+TGG + chr4.2:29819077-29819096 MS.gene045476:CDS 45.0%
TGATGTGGGACCATAGTAAG+AGG - chr4.2:29818807-29818826 None:intergenic 45.0%
TGCGGTAAGGGTTTCTTGTA+TGG + chr4.2:29818654-29818673 MS.gene045476:CDS 45.0%
TTTGGGAGCAGATTTGGAGA+TGG + chr4.2:29818266-29818285 MS.gene045476:CDS 45.0%
! CTTGATGACATCACAGGAAG+AGG - chr4.2:29818982-29819001 None:intergenic 45.0%
GAAACCCTTACCGCAAAAGG+AGG - chr4.2:29818649-29818668 None:intergenic 50.0%
GGCACGACAAATTGATTGCC+TGG + chr4.2:29818583-29818602 MS.gene045476:CDS 50.0%
TGTCGTGCCAAGTTTCGTCT+GGG - chr4.2:29818572-29818591 None:intergenic 50.0%
TTGTCGTGCCAAGTTTCGTC+TGG - chr4.2:29818573-29818592 None:intergenic 50.0%
!! TGGAGATGGAGGTGTTGTTG+CGG + chr4.2:29818280-29818299 MS.gene045476:CDS 50.0%
GGGAGCAGATTTGGAGATGG+AGG + chr4.2:29818269-29818288 MS.gene045476:CDS 55.0%
! GCTCCCTCCTTTTGCGGTAA+GGG + chr4.2:29818642-29818661 MS.gene045476:CDS 55.0%
! TGCTCCCTCCTTTTGCGGTA+AGG + chr4.2:29818641-29818660 MS.gene045476:CDS 55.0%
! CCTCGTGCTCCCTCCTTTTG+CGG + chr4.2:29818636-29818655 MS.gene045476:CDS 60.0%
! CCGCAAAAGGAGGGAGCACG+AGG - chr4.2:29818639-29818658 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 29818261 29819133 29818261 ID=MS.gene045476
chr4.2 mRNA 29818261 29819133 29818261 ID=MS.gene045476.t1;Parent=MS.gene045476
chr4.2 exon 29818261 29819133 29818261 ID=MS.gene045476.t1.exon1;Parent=MS.gene045476.t1
chr4.2 CDS 29818261 29819133 29818261 ID=cds.MS.gene045476.t1;Parent=MS.gene045476.t1
Gene Sequence

>MS.gene045476

ATGAATTTGGGAGCAGATTTGGAGATGGAGGTGTTGTTGCGGCTACAAGTAAAATCATTGATGAGGTTCAAATGTGTTCAAAAATCATGGAACAATCTCATCAAAAGTCCTTACTTTGTGAATAGAAGATGCAAGTTGCTCATCCCTGAAAACCCTATTCTTCTAGTCATTGAAAAATCCAAATTGAAACTTCTCTCATGTGACGGTGACAATGAGAAACCAATCCTCATTAAGTCCTTTACCAAATACATAGAAAGAATTGAATCTTACGGTAGTTGCAACGGTGTTTTTTGTCTCAAAGTTATTTACCCAGACGAAACTTGGCACGACAAATTGATTGCCTGGAACCCAACAACTAATGAGATGCATCATATTCCTCGTGCTCCCTCCTTTTGCGGTAAGGGTTTCTTGTATGGTTTTGGTGCTGTTAACGACGACTTCAAAGTTGTCAAACTCAATATCAATGTGACGACAAAAATGCTTTATCCTTTGGTTGAAGTTTATAATCTAAGCACTAAATCCTGGAAAATTATCTACAATTGTCCTCTTACTATGGTCCCACATCAACGTCCTTCAAGATATAATGCTCTTGTTAATGGTGTTTATCATTGGATTACTCGCTCGACACAATATAATAATGCTCCAAACATACTCTGCTTTAATTTTCGCAATAACAAGTTTCAGCAACTTCAAGCTCCAAACTCACATCACAGTATGCCCTCTTCCTGTGATGTCATCAAGATAAAAGGTTCCCTTGGTTATGCTATCATGCTATGCAATATCAAAACTGTTTGTATATGTGGTTGCTGGATATATGGGTTATGGATCAAAACAGGTGGACTAAAAAGTATAGGATTAAACCAGATATGTTGA
Protein sequence

>MS.gene045476.t1

MNLGADLEMEVLLRLQVKSLMRFKCVQKSWNNLIKSPYFVNRRCKLLIPENPILLVIEKSKLKLLSCDGDNEKPILIKSFTKYIERIESYGSCNGVFCLKVIYPDETWHDKLIAWNPTTNEMHHIPRAPSFCGKGFLYGFGAVNDDFKVVKLNINVTTKMLYPLVEVYNLSTKSWKIIYNCPLTMVPHQRPSRYNALVNGVYHWITRSTQYNNAPNILCFNFRNNKFQQLQAPNSHHSMPSSCDVIKIKGSLGYAIMLCNIKTVCICGCWIYGLWIKTGGLKSIGLNQIC