AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035261


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035261.t1 MTR_4g118380 94.262 122 7 0 1 122 236 357 5.41e-81 243
MS.gene035261.t1 MTR_4g118400 73.427 143 17 1 1 143 173 294 2.33e-67 206
MS.gene035261.t1 MTR_6g060650 71.329 143 32 1 1 143 234 367 5.89e-66 204
MS.gene035261.t1 MTR_4g117720 67.347 147 34 2 2 143 234 371 2.97e-61 193
MS.gene035261.t1 MTR_3g103020 62.759 145 43 2 1 143 237 372 9.16e-55 176
MS.gene035261.t1 MTR_4g109280 62.676 142 41 3 1 140 219 350 4.72e-54 174
MS.gene035261.t1 MTR_4g109270 58.621 145 49 2 1 143 223 358 1.17e-49 162
MS.gene035261.t1 MTR_3g034180 59.441 143 48 2 1 143 351 483 1.38e-49 165
MS.gene035261.t1 MTR_3g034130 57.639 144 51 2 1 144 237 370 1.13e-48 160
MS.gene035261.t1 MTR_4g117850 59.859 142 45 3 2 140 239 371 1.53e-47 157
MS.gene035261.t1 MTR_4g122850 59.028 144 46 5 3 143 225 358 1.56e-46 155
MS.gene035261.t1 MTR_8g040935 55.479 146 52 3 2 143 66 202 5.14e-45 146
MS.gene035261.t1 MTR_4g094365 54.795 146 54 4 1 143 222 358 1.35e-44 149
MS.gene035261.t1 MTR_3g034160 58.741 143 49 2 1 143 237 369 3.20e-44 149
MS.gene035261.t1 MTR_5g097210 55.556 144 52 3 2 144 226 358 7.27e-44 148
MS.gene035261.t1 MTR_4g116330 53.793 145 54 3 3 143 239 374 7.39e-44 148
MS.gene035261.t1 MTR_4g116320 56.164 146 49 5 2 143 118 252 3.18e-43 143
MS.gene035261.t1 MTR_4g117870 61.789 123 45 2 22 143 140 261 3.16e-42 141
MS.gene035261.t1 MTR_1g055175 53.472 144 53 5 3 143 63 195 7.79e-42 138
MS.gene035261.t1 MTR_1g055195 53.147 143 58 3 1 143 339 472 3.63e-41 143
MS.gene035261.t1 MTR_4g118360 61.261 111 39 2 35 143 12 120 9.51e-41 132
MS.gene035261.t1 MTR_1g055165 53.472 144 53 5 3 143 180 312 1.94e-40 137
MS.gene035261.t1 MTR_4g023130 53.521 142 52 3 1 140 175 304 4.28e-40 136
MS.gene035261.t1 MTR_4g023090 48.408 157 56 4 2 143 232 378 1.22e-39 137
MS.gene035261.t1 MTR_4g118370 49.640 139 59 2 6 144 128 255 1.26e-39 134
MS.gene035261.t1 MTR_4g023380 46.951 164 54 3 3 143 218 371 1.49e-39 137
MS.gene035261.t1 MTR_1g055135 53.147 143 58 4 3 143 234 369 1.80e-39 136
MS.gene035261.t1 MTR_4g023080 52.817 142 53 4 1 140 216 345 3.70e-39 135
MS.gene035261.t1 MTR_8g433030 55.102 147 49 6 1 143 203 336 5.57e-39 134
MS.gene035261.t1 MTR_8g032390 55.102 147 49 6 1 143 236 369 1.06e-38 134
MS.gene035261.t1 MTR_8g433040 55.944 143 49 4 3 143 228 358 2.03e-38 133
MS.gene035261.t1 MTR_8g032380 55.944 143 49 4 3 143 228 358 2.03e-38 133
MS.gene035261.t1 MTR_4g117750 55.814 129 54 2 17 143 350 477 3.06e-38 135
MS.gene035261.t1 MTR_4g117750 52.113 142 64 2 3 144 217 354 5.44e-35 126
MS.gene035261.t1 MTR_5g070130 49.655 145 61 3 2 143 235 370 1.32e-36 129
MS.gene035261.t1 MTR_4g117770 49.306 144 65 2 2 143 224 361 1.25e-35 126
MS.gene035261.t1 MTR_4g117810 49.306 144 65 2 2 143 224 361 1.25e-35 126
MS.gene035261.t1 MTR_4g117780 48.611 144 66 2 2 143 224 361 2.05e-35 125
MS.gene035261.t1 MTR_4g021785 47.887 142 60 3 1 140 217 346 3.65e-35 125
MS.gene035261.t1 MTR_5g070080 51.034 145 59 3 1 143 245 379 6.95e-35 124
MS.gene035261.t1 MTR_4g023070 47.403 154 57 4 1 140 218 361 1.39e-34 124
MS.gene035261.t1 MTR_4g023190 50.000 148 59 6 1 143 216 353 5.95e-34 121
MS.gene035261.t1 MTR_4g023120 47.742 155 55 5 1 140 218 361 9.23e-34 121
MS.gene035261.t1 MTR_5g070088 46.897 145 67 2 1 143 243 379 8.69e-33 119
MS.gene035261.t1 MTR_1g055250 50.000 140 53 6 4 141 133 257 1.11e-32 117
MS.gene035261.t1 MTR_5g023000 61.798 89 34 0 55 143 5 93 1.18e-32 111
MS.gene035261.t1 MTR_4g023150 48.649 148 61 6 1 143 217 354 2.06e-32 117
MS.gene035261.t1 MTR_8g102520 45.139 144 69 4 1 143 182 316 2.43e-31 114
MS.gene035261.t1 MTR_6g463430 48.611 144 57 5 3 143 67 196 3.18e-31 110
MS.gene035261.t1 MTR_4g023440 48.760 121 49 2 23 143 219 326 6.39e-31 113
MS.gene035261.t1 MTR_4g023200 47.297 148 63 6 1 143 217 354 1.04e-30 113
MS.gene035261.t1 MTR_7g079450 45.833 144 64 4 1 143 1 131 1.32e-30 107
MS.gene035261.t1 MTR_4g122860 50.000 122 52 3 28 143 196 314 2.99e-30 111
MS.gene035261.t1 MTR_4g116330 54.206 107 45 2 41 143 229 335 9.58e-29 107
MS.gene035261.t1 MTR_7g079400 42.177 147 71 3 2 143 194 331 1.13e-28 107
MS.gene035261.t1 MTR_7g079370 45.833 144 67 3 1 143 217 350 3.78e-27 103
MS.gene035261.t1 MTR_4g117530 40.000 135 74 2 9 143 120 247 3.73e-26 99.4
MS.gene035261.t1 MTR_4g114700 54.167 96 37 2 51 139 122 217 2.36e-25 97.1
MS.gene035261.t1 MTR_7g079420 42.466 146 69 6 2 143 1004 1138 1.19e-24 99.0
MS.gene035261.t1 MTR_8g098830 43.333 120 58 3 1 120 87 196 8.00e-24 92.8
MS.gene035261.t1 MTR_7g117720 53.684 95 40 2 54 144 178 272 1.26e-22 90.5
MS.gene035261.t1 MTR_4g121580 42.105 133 56 5 10 140 227 340 2.56e-19 82.8
MS.gene035261.t1 MTR_2g048580 38.667 150 60 10 2 143 211 336 1.93e-15 72.0
MS.gene035261.t1 MTR_8g013005 62.264 53 20 0 91 143 1 53 4.32e-15 65.5
MS.gene035261.t1 MTR_4g023430 46.667 75 37 1 72 143 142 216 1.84e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 27 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGATGATATAGTTGAAATA+TGG 0.254965 8.1:+9323836 MS.gene035261:CDS
GGTTGTGATTTGTGGGTATT+TGG 0.288331 8.1:+9323787 MS.gene035261:CDS
GGCTATGTATACAGAGTCTT+TGG 0.303429 8.1:+9324071 MS.gene035261:CDS
TGTGCGATTCTCTTTATAAT+AGG 0.305240 8.1:-9324040 None:intergenic
TTGGTGAGTTTCTCAGTGTA+TGG 0.351491 8.1:+9323806 MS.gene035261:CDS
AACCTACAGGTTGTGATTTG+TGG 0.451176 8.1:+9323779 MS.gene035261:CDS
ATTGGAACTGATGGTGAAGT+TGG 0.456865 8.1:+9323973 MS.gene035261:CDS
TTTCCTTTCAATTAAATCAA+AGG 0.478785 8.1:-9323692 None:intergenic
GGATGATATAGTTGAAATAT+GGG 0.480581 8.1:+9323837 MS.gene035261:CDS
TTGCCTTTGATTTAATTGAA+AGG 0.515684 8.1:+9323689 MS.gene035261:CDS
ACCTACAGGTTGTGATTTGT+GGG 0.529599 8.1:+9323780 MS.gene035261:CDS
GCGATTCTCTTTATAATAGG+AGG 0.531668 8.1:-9324037 None:intergenic
TTGGTGAAGTATGATGAAAA+AGG 0.538205 8.1:+9323997 MS.gene035261:CDS
CTCTGTATACATAGCCACTT+GGG 0.543827 8.1:-9324064 None:intergenic
AACTGATGGTGAAGTTGGAT+TGG 0.547756 8.1:+9323978 MS.gene035261:CDS
TAAACTTGTTCGCTATCACC+GGG 0.548745 8.1:-9324103 None:intergenic
TCTCAGTGTATGGACTAAGC+AGG 0.562084 8.1:+9323816 MS.gene035261:CDS
ACTCTGTATACATAGCCACT+TGG 0.572904 8.1:-9324065 None:intergenic
GGTGATATTATTGGAACTGA+TGG 0.578976 8.1:+9323964 MS.gene035261:CDS
AGTACCAAGTGCATTCGTCA+TGG 0.586111 8.1:+9323869 MS.gene035261:CDS
TAGTCCATGACGAATGCACT+TGG 0.621108 8.1:-9323873 None:intergenic
ATTGGAGCGAAGTAATGAGT+GGG 0.631785 8.1:-9323925 None:intergenic
TATTGGAGCGAAGTAATGAG+TGG 0.644959 8.1:-9323926 None:intergenic
GGTTCATGTTCAAAATCACC+TGG 0.645701 8.1:-9323760 None:intergenic
AAACTTGTTCGCTATCACCG+GGG 0.668208 8.1:-9324102 None:intergenic
AGAGAATCGCACATCCCAAG+TGG 0.670773 8.1:+9324050 MS.gene035261:CDS
ACCCACAAATCACAACCTGT+AGG 0.682592 8.1:-9323781 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCCTTTCAATTAAATCAA+AGG - chr8.1:9323695-9323714 None:intergenic 20.0%
! ACAAAAAGTGGTGATATTAT+TGG + chr8.1:9323955-9323974 MS.gene035261:CDS 25.0%
! AGGATGATATAGTTGAAATA+TGG + chr8.1:9323836-9323855 MS.gene035261:CDS 25.0%
! GGATGATATAGTTGAAATAT+GGG + chr8.1:9323837-9323856 MS.gene035261:CDS 25.0%
!! TTGCCTTTGATTTAATTGAA+AGG + chr8.1:9323689-9323708 MS.gene035261:CDS 25.0%
CCAATATGTAGTACAAAAAG+TGG + chr8.1:9323943-9323962 MS.gene035261:CDS 30.0%
TGTGCGATTCTCTTTATAAT+AGG - chr8.1:9324043-9324062 None:intergenic 30.0%
TTGGTGAAGTATGATGAAAA+AGG + chr8.1:9323997-9324016 MS.gene035261:CDS 30.0%
!!! CCACTTTTTGTACTACATAT+TGG - chr8.1:9323946-9323965 None:intergenic 30.0%
GCGATTCTCTTTATAATAGG+AGG - chr8.1:9324040-9324059 None:intergenic 35.0%
GGTGATATTATTGGAACTGA+TGG + chr8.1:9323964-9323983 MS.gene035261:CDS 35.0%
! GATTTTGAACATGAACCTAC+AGG + chr8.1:9323766-9323785 MS.gene035261:CDS 35.0%
!!! CTTTGCCAGATGATCTTTTT+TGG + chr8.1:9323728-9323747 MS.gene035261:CDS 35.0%
AACCTACAGGTTGTGATTTG+TGG + chr8.1:9323779-9323798 MS.gene035261:CDS 40.0%
ACTCTGTATACATAGCCACT+TGG - chr8.1:9324068-9324087 None:intergenic 40.0%
ATTGGAGCGAAGTAATGAGT+GGG - chr8.1:9323928-9323947 None:intergenic 40.0%
CCTCTCCAAAAAAGATCATC+TGG - chr8.1:9323736-9323755 None:intergenic 40.0%
CTCTGTATACATAGCCACTT+GGG - chr8.1:9324067-9324086 None:intergenic 40.0%
GGCTATGTATACAGAGTCTT+TGG + chr8.1:9324071-9324090 MS.gene035261:CDS 40.0%
GGTTCATGTTCAAAATCACC+TGG - chr8.1:9323763-9323782 None:intergenic 40.0%
TATTGGAGCGAAGTAATGAG+TGG - chr8.1:9323929-9323948 None:intergenic 40.0%
TTGGTGAGTTTCTCAGTGTA+TGG + chr8.1:9323806-9323825 MS.gene035261:CDS 40.0%
! AACTGATGGTGAAGTTGGAT+TGG + chr8.1:9323978-9323997 MS.gene035261:CDS 40.0%
! ACCTACAGGTTGTGATTTGT+GGG + chr8.1:9323780-9323799 MS.gene035261:CDS 40.0%
! ATTGGAACTGATGGTGAAGT+TGG + chr8.1:9323973-9323992 MS.gene035261:CDS 40.0%
! GGTTGTGATTTGTGGGTATT+TGG + chr8.1:9323787-9323806 MS.gene035261:CDS 40.0%
!!! CCAGATGATCTTTTTTGGAG+AGG + chr8.1:9323733-9323752 MS.gene035261:CDS 40.0%
ACCCACAAATCACAACCTGT+AGG - chr8.1:9323784-9323803 None:intergenic 45.0%
AGTACCAAGTGCATTCGTCA+TGG + chr8.1:9323869-9323888 MS.gene035261:CDS 45.0%
TAGTCCATGACGAATGCACT+TGG - chr8.1:9323876-9323895 None:intergenic 45.0%
! TCTCAGTGTATGGACTAAGC+AGG + chr8.1:9323816-9323835 MS.gene035261:CDS 45.0%
!!! AGTCTTTGGTTTTCACTCCC+CGG + chr8.1:9324085-9324104 MS.gene035261:CDS 45.0%
!!! CTTTTTTGGAGAGGTTTGCC+AGG + chr8.1:9323742-9323761 MS.gene035261:CDS 45.0%
AGAGAATCGCACATCCCAAG+TGG + chr8.1:9324050-9324069 MS.gene035261:CDS 50.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 9323676 9324110 9323676 ID=MS.gene035261
chr8.1 mRNA 9323676 9324110 9323676 ID=MS.gene035261.t1;Parent=MS.gene035261
chr8.1 exon 9323676 9324110 9323676 ID=MS.gene035261.t1.exon1;Parent=MS.gene035261.t1
chr8.1 CDS 9323676 9324110 9323676 ID=cds.MS.gene035261.t1;Parent=MS.gene035261.t1
Gene Sequence

>MS.gene035261

ATGAATGTTATTGTTGCCTTTGATTTAATTGAAAGGAAACTTTTAGACATATCTTTGCCAGATGATCTTTTTTGGAGAGGTTTGCCAGGTGATTTTGAACATGAACCTACAGGTTGTGATTTGTGGGTATTTGGTGAGTTTCTCAGTGTATGGACTAAGCAGGATGATATAGTTGAAATATGGGTGATGAAAGAGTACCAAGTGCATTCGTCATGGACTAAGACTCTTGTTTTTTCAATTGATGTCATTCCCACTCATTACTTCGCTCCAATATGTAGTACAAAAAGTGGTGATATTATTGGAACTGATGGTGAAGTTGGATTGGTGAAGTATGATGAAAAAGGACAGTTTCTAGAGTATACCTCCTATTATAAAGAGAATCGCACATCCCAAGTGGCTATGTATACAGAGTCTTTGGTTTTCACTCCCCGGTGA

Protein sequence

>MS.gene035261.t1

MNVIVAFDLIERKLLDISLPDDLFWRGLPGDFEHEPTGCDLWVFGEFLSVWTKQDDIVEIWVMKEYQVHSSWTKTLVFSIDVIPTHYFAPICSTKSGDIIGTDGEVGLVKYDEKGQFLEYTSYYKENRTSQVAMYTESLVFTPR