AlfalfaGEDB | MS.gene27285

M. sativa cultivar XinJiangDaYe / MS.gene27285

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27285.t1	RHN77540.1	93.1	217	15	0	34	250	9	225	3.00E-119	438

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27285.t1	A0A396JH16	93.1	217	15	0	34	250	9	225	2.2e-119	438.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene27285	MS.gene90742	PPI
MS.gene041388	MS.gene27285	PPI
MS.gene27285	MS.gene24212	PPI
MS.gene27285	MS.gene50332	PPI
MS.gene062698	MS.gene27285	PPI
MS.gene57536	MS.gene27285	PPI
MS.gene96126	MS.gene27285	PPI
MS.gene019503	MS.gene27285	PPI
MS.gene016524	MS.gene27285	PPI
MS.gene50304	MS.gene27285	PPI
MS.gene27285	MS.gene58240	PPI
MS.gene27285	MS.gene063472	PPI
MS.gene27285	MS.gene43790	PPI
MS.gene27285	MS.gene57717	PPI
MS.gene27285	MS.gene034680	PPI
MS.gene31587	MS.gene27285	PPI
MS.gene27285	MS.gene044563	PPI
MS.gene036557	MS.gene27285	PPI
MS.gene041393	MS.gene27285	PPI
MS.gene033441	MS.gene27285	PPI
MS.gene27285	MS.gene50327	PPI
MS.gene27285	MS.gene066626	PPI
MS.gene27285	MS.gene40982	PPI
MS.gene27285	MS.gene063474	PPI
MS.gene063826	MS.gene27285	PPI
MS.gene27285	MS.gene88416	PPI
MS.gene27285	MS.gene57530	PPI
MS.gene27285	MS.gene034681	PPI
MS.gene84698	MS.gene27285	PPI
MS.gene44892	MS.gene27285	PPI
MS.gene27285	MS.gene57532	PPI
MS.gene27285	MS.gene003076	PPI
MS.gene58239	MS.gene27285	PPI
MS.gene066641	MS.gene27285	PPI
MS.gene000038	MS.gene27285	PPI
MS.gene27285	MS.gene009892	PPI
MS.gene57531	MS.gene27285	PPI
MS.gene27285	MS.gene061241	PPI
MS.gene27285	MS.gene058669	PPI
MS.gene39262	MS.gene27285	PPI
MS.gene27285	MS.gene51192	PPI
MS.gene57535	MS.gene27285	PPI
MS.gene025887	MS.gene27285	PPI
MS.gene27285	MS.gene51188	PPI
MS.gene27285	MS.gene033875	PPI
MS.gene041470	MS.gene27285	PPI
MS.gene058694	MS.gene27285	PPI
MS.gene27285	MS.gene041489	PPI
MS.gene27285	MS.gene38220	PPI
MS.gene27285	MS.gene40986	PPI
MS.gene27285	MS.gene034688	PPI
MS.gene015089	MS.gene27285	PPI
MS.gene006710	MS.gene27285	PPI
MS.gene27285	MS.gene03060	PPI
MS.gene27285	MS.gene24213	PPI
MS.gene27285	MS.gene033440	PPI
MS.gene27285	MS.gene024011	PPI
MS.gene44885	MS.gene27285	PPI
MS.gene27285	MS.gene034677	PPI
MS.gene011679	MS.gene27285	PPI
MS.gene27285	MS.gene064109	PPI
MS.gene060370	MS.gene27285	PPI
MS.gene27285	MS.gene035238	PPI
MS.gene64001	MS.gene27285	PPI
MS.gene27285	MS.gene85043	PPI
MS.gene27285	MS.gene064114	PPI
MS.gene57533	MS.gene27285	PPI
MS.gene27285	MS.gene87584	PPI
MS.gene032970	MS.gene27285	PPI
MS.gene27285	MS.gene062698	PPI
MS.gene27285	MS.gene80610	PPI
MS.gene77856	MS.gene27285	PPI
MS.gene40887	MS.gene27285	PPI
MS.gene27285	MS.gene90743	PPI
MS.gene27285	MS.gene40886	PPI
MS.gene060366	MS.gene27285	PPI
MS.gene27285	MS.gene85454	PPI
MS.gene27285	MS.gene041391	PPI
MS.gene27285	MS.gene033443	PPI
MS.gene57529	MS.gene27285	PPI
MS.gene27285	MS.gene57536	PPI
MS.gene80884	MS.gene27285	PPI
MS.gene27285	MS.gene058701	PPI
MS.gene40902	MS.gene27285	PPI
MS.gene27285	MS.gene87554	PPI
MS.gene27285	MS.gene009891	PPI
MS.gene27285	MS.gene064083	PPI
MS.gene27285	MS.gene27051	PPI
MS.gene27285	MS.gene048193	PPI
MS.gene033875	MS.gene27285	PPI
MS.gene041474	MS.gene27285	PPI
MS.gene27285	MS.gene024420	PPI
MS.gene27285	MS.gene016220	PPI
MS.gene27020	MS.gene27285	PPI
MS.gene27285	MS.gene024008	PPI
MS.gene27285	MS.gene50337	PPI
MS.gene066620	MS.gene27285	PPI
MS.gene035236	MS.gene27285	PPI
MS.gene27285	MS.gene54541	PPI
MS.gene27285	MS.gene39265	PPI
MS.gene27285	MS.gene060368	PPI
MS.gene27285	MS.gene053299	PPI
MS.gene51208	MS.gene27285	PPI
MS.gene27285	MS.gene28758	PPI
MS.gene27285	MS.gene044429	PPI
MS.gene011106	MS.gene27285	PPI
MS.gene62398	MS.gene27285	PPI
MS.gene60027	MS.gene27285	PPI
MS.gene27285	MS.gene27284	PPI
MS.gene045475	MS.gene27285	PPI
MS.gene88776	MS.gene27285	PPI
MS.gene35068	MS.gene27285	PPI
MS.gene041390	MS.gene27285	PPI
MS.gene90744	MS.gene27285	PPI
MS.gene51204	MS.gene27285	PPI
MS.gene92376	MS.gene27285	PPI
MS.gene27285	MS.gene48221	PPI
MS.gene000035	MS.gene27285	PPI
MS.gene50329	MS.gene27285	PPI
MS.gene31637	MS.gene27285	PPI
MS.gene87573	MS.gene27285	PPI
MS.gene57537	MS.gene27285	PPI
MS.gene27285	MS.gene51190	PPI
MS.gene019504	MS.gene27285	PPI
MS.gene27285	MS.gene034762	PPI
MS.gene27285	MS.gene58319	PPI
MS.gene27285	MS.gene59943	PPI
MS.gene27285	MS.gene044426	PPI
MS.gene059156	MS.gene27285	PPI
MS.gene27285	MS.gene033442	PPI
MS.gene82082	MS.gene27285	PPI
MS.gene033444	MS.gene27285	PPI
MS.gene50306	MS.gene27285	PPI
MS.gene27285	MS.gene44428	PPI
MS.gene27285	MS.gene066120	PPI
MS.gene26949	MS.gene27285	PPI
MS.gene27285	MS.gene035235	PPI
MS.gene27285	MS.gene058703	PPI
MS.gene87200	MS.gene27285	PPI
MS.gene58311	MS.gene27285	PPI
MS.gene27285	MS.gene024000	PPI
MS.gene27285	MS.gene012215	PPI
MS.gene033432	MS.gene27285	PPI
MS.gene27285	MS.gene033079	PPI
MS.gene59639	MS.gene27285	PPI
MS.gene27285	MS.gene055694	PPI
MS.gene27285	MS.gene059101	PPI
MS.gene066635	MS.gene27285	PPI
MS.gene27285	MS.gene58316	PPI
MS.gene27285	MS.gene033430	PPI
MS.gene51192	MS.gene27285	PPI
MS.gene27285	MS.gene58321	PPI
MS.gene010758	MS.gene27285	PPI
MS.gene27285	MS.gene87564	PPI
MS.gene27285	MS.gene58315	PPI
MS.gene87586	MS.gene27285	PPI
MS.gene50326	MS.gene27285	PPI
MS.gene51200	MS.gene27285	PPI
MS.gene50339	MS.gene27285	PPI
MS.gene27285	MS.gene041473	PPI
MS.gene27285	MS.gene50311	PPI
MS.gene88774	MS.gene27285	PPI
MS.gene27285	MS.gene064112	PPI
MS.gene27285	MS.gene058989	PPI
MS.gene27285	MS.gene036556	PPI
MS.gene27285	MS.gene57538	PPI
MS.gene27285	MS.gene060367	PPI
MS.gene27285	MS.gene058693	PPI
MS.gene27285	MS.gene27319	PPI
MS.gene42452	MS.gene27285	PPI
MS.gene27285	MS.gene041395	PPI
MS.gene063414	MS.gene27285	PPI
MS.gene27285	MS.gene51200	PPI
MS.gene27285	MS.gene51189	PPI
MS.gene27285	MS.gene046958	PPI
MS.gene27285	MS.gene24286	PPI
MS.gene87563	MS.gene27285	PPI
MS.gene57526	MS.gene27285	PPI
MS.gene021553	MS.gene27285	PPI
MS.gene27285	MS.gene58783	PPI
MS.gene051820	MS.gene27285	PPI
MS.gene26957	MS.gene27285	PPI
MS.gene97996	MS.gene27285	PPI
MS.gene036582	MS.gene27285	PPI
MS.gene27021	MS.gene27285	PPI
MS.gene27285	MS.gene40988	PPI
MS.gene58320	MS.gene27285	PPI
MS.gene26101	MS.gene27285	PPI
MS.gene27285	MS.gene24173	PPI
MS.gene27283	MS.gene27285	PPI
MS.gene27285	MS.gene036584	PPI
MS.gene27285	MS.gene036662	PPI
MS.gene58781	MS.gene27285	PPI
MS.gene27285	MS.gene42451	PPI
MS.gene44886	MS.gene27285	PPI
MS.gene27285	MS.gene035261	PPI
MS.gene87562	MS.gene27285	PPI
MS.gene27285	MS.gene023999	PPI
MS.gene038756	MS.gene27285	PPI
MS.gene40904	MS.gene27285	PPI
MS.gene27285	MS.gene69307	PPI
MS.gene87569	MS.gene27285	PPI
MS.gene27285	MS.gene036581	PPI
MS.gene27285	MS.gene019507	PPI
MS.gene27285	MS.gene033431	PPI
MS.gene041491	MS.gene27285	PPI
MS.gene27285	MS.gene50320	PPI
MS.gene059096	MS.gene27285	PPI
MS.gene57528	MS.gene27285	PPI
MS.gene27285	MS.gene58314	PPI
MS.gene27285	MS.gene87557	PPI
MS.gene50297	MS.gene27285	PPI
MS.gene27285	MS.gene51199	PPI
MS.gene27285	MS.gene035263	PPI
MS.gene27285	MS.gene67501	PPI
MS.gene006711	MS.gene27285	PPI
MS.gene27285	MS.gene96406	PPI
MS.gene58716	MS.gene27285	PPI
MS.gene27285	MS.gene035262	PPI
MS.gene019505	MS.gene27285	PPI
MS.gene045476	MS.gene27285	PPI
MS.gene58785	MS.gene27285	PPI
MS.gene27285	MS.gene028390	PPI
MS.gene24208	MS.gene27285	PPI
MS.gene50307	MS.gene27285	PPI
MS.gene27285	MS.gene40878	PPI
MS.gene034690	MS.gene27285	PPI
MS.gene27285	MS.gene96407	PPI
MS.gene066639	MS.gene27285	PPI
MS.gene96407	MS.gene27285	PPI
MS.gene036760	MS.gene27285	PPI
MS.gene77857	MS.gene27285	PPI
MS.gene27285	MS.gene047279	PPI
MS.gene27285	MS.gene060370	PPI
MS.gene27285	MS.gene50305	PPI
MS.gene57527	MS.gene27285	PPI
MS.gene51191	MS.gene27285	PPI
MS.gene27285	MS.gene024002	PPI
MS.gene27285	MS.gene032969	PPI
MS.gene27285	MS.gene68890	PPI
MS.gene033426	MS.gene27285	PPI
MS.gene27285	MS.gene50297	PPI
MS.gene27285	MS.gene42445	PPI
MS.gene50298	MS.gene27285	PPI
MS.gene27285	MS.gene86273	PPI
MS.gene67687	MS.gene27285	PPI
MS.gene27285	MS.gene26830	PPI
MS.gene27285	MS.gene40985	PPI
MS.gene27285	MS.gene22715	PPI
MS.gene27285	MS.gene038756	PPI
MS.gene27285	MS.gene51207	PPI
MS.gene27285	MS.gene63657	PPI
MS.gene27285	MS.gene059157	PPI
MS.gene063670	MS.gene27285	PPI
MS.gene27285	MS.gene36595	PPI
MS.gene27285	MS.gene024004	PPI
MS.gene71159	MS.gene27285	PPI
MS.gene27285	MS.gene045140	PPI
MS.gene019506	MS.gene27285	PPI
MS.gene27285	MS.gene070462	PPI
MS.gene058692	MS.gene27285	PPI
MS.gene27285	MS.gene061240	PPI
MS.gene69309	MS.gene27285	PPI
MS.gene63658	MS.gene27285	PPI
MS.gene024002	MS.gene27285	PPI
MS.gene033081	MS.gene27285	PPI
MS.gene27285	MS.gene064086	PPI
MS.gene27285	MS.gene035234	PPI
MS.gene27285	MS.gene041468	PPI
MS.gene034686	MS.gene27285	PPI
MS.gene40982	MS.gene27285	PPI
MS.gene070461	MS.gene27285	PPI
MS.gene51199	MS.gene27285	PPI
MS.gene27285	MS.gene066639	PPI
MS.gene27285	MS.gene42447	PPI
MS.gene27285	MS.gene024979	PPI
MS.gene87559	MS.gene27285	PPI
MS.gene27285	MS.gene24826	PPI
MS.gene27285	MS.gene044425	PPI
MS.gene041475	MS.gene27285	PPI
MS.gene81242	MS.gene27285	PPI
MS.gene27285	MS.gene59637	PPI
MS.gene64203	MS.gene27285	PPI
MS.gene27285	MS.gene27022	PPI
MS.gene060367	MS.gene27285	PPI
MS.gene42444	MS.gene27285	PPI
MS.gene51202	MS.gene27285	PPI
MS.gene27285	MS.gene87585	PPI
MS.gene27285	MS.gene024009	PPI
MS.gene27285	MS.gene036599	PPI
MS.gene27285	MS.gene40887	PPI
MS.gene064111	MS.gene27285	PPI
MS.gene27285	MS.gene006705	PPI
MS.gene26829	MS.gene27285	PPI
MS.gene064104	MS.gene27285	PPI
MS.gene053256	MS.gene27285	PPI
MS.gene27285	MS.gene035237	PPI
MS.gene75875	MS.gene27285	PPI
MS.gene27285	MS.gene000036	PPI
MS.gene54049	MS.gene27285	PPI
MS.gene43789	MS.gene27285	PPI
MS.gene27285	MS.gene50309	PPI
MS.gene27285	MS.gene041394	PPI
MS.gene27285	MS.gene036664	PPI
MS.gene072177	MS.gene27285	PPI
MS.gene58722	MS.gene27285	PPI
MS.gene27285	MS.gene053257	PPI
MS.gene27285	MS.gene58241	PPI
MS.gene064091	MS.gene27285	PPI
MS.gene27285	MS.gene77858	PPI
MS.gene064112	MS.gene27285	PPI
MS.gene27285	MS.gene51201	PPI
MS.gene27285	MS.gene058696	PPI
MS.gene27285	MS.gene009893	PPI
MS.gene27285	MS.gene50336	PPI
MS.gene43796	MS.gene27285	PPI
MS.gene27285	MS.gene77704	PPI
MS.gene58312	MS.gene27285	PPI
MS.gene36704	MS.gene27285	PPI
MS.gene27285	MS.gene033083	PPI
MS.gene58313	MS.gene27285	PPI
MS.gene066622	MS.gene27285	PPI
MS.gene44887	MS.gene27285	PPI
MS.gene63655	MS.gene27285	PPI
MS.gene033430	MS.gene27285	PPI
MS.gene44883	MS.gene27285	PPI
MS.gene27285	MS.gene036663	PPI
MS.gene27285	MS.gene68580	PPI
MS.gene036583	MS.gene27285	PPI
MS.gene88780	MS.gene27285	PPI
MS.gene27285	MS.gene57525	PPI
MS.gene41046	MS.gene27285	PPI
MS.gene87588	MS.gene27285	PPI
MS.gene017412	MS.gene27285	PPI
MS.gene27285	MS.gene023996	PPI
MS.gene27285	MS.gene030114	PPI
MS.gene27285	MS.gene045473	PPI
MS.gene27285	MS.gene50301	PPI
MS.gene96448	MS.gene27285	PPI
MS.gene27285	MS.gene88774	PPI
MS.gene009883	MS.gene27285	PPI
MS.gene27285	MS.gene045474	PPI
MS.gene041392	MS.gene27285	PPI
MS.gene27285	MS.gene031590	PPI
MS.gene27285	MS.gene036558	PPI
MS.gene044563	MS.gene27285	PPI
MS.gene069075	MS.gene27285	PPI
MS.gene033427	MS.gene27285	PPI
MS.gene27285	MS.gene40984	PPI
MS.gene27285	MS.gene066623	PPI
MS.gene27285	MS.gene064108	PPI
MS.gene40985	MS.gene27285	PPI
MS.gene031591	MS.gene27285	PPI
MS.gene27285	MS.gene064090	PPI
MS.gene27285	MS.gene24208	PPI
MS.gene27285	MS.gene58317	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27285.t1	MTR_1g023400	71.837	245	57	4	1	242	117	352	1.09e-121	350
MS.gene27285.t1	MTR_1g023430	92.105	152	12	0	99	250	29	180	2.12e-103	298
MS.gene27285.t1	MTR_1g022200	63.035	257	83	5	3	250	127	380	2.42e-103	305
MS.gene27285.t1	MTR_4g080200	47.791	249	115	7	1	242	109	349	7.83e-67	211
MS.gene27285.t1	MTR_1g009110	48.193	249	117	7	1	243	137	379	8.46e-65	206
MS.gene27285.t1	MTR_1g024125	69.492	118	32	2	115	228	38	155	1.15e-53	171
MS.gene27285.t1	MTR_1g037540	45.833	264	110	12	1	242	148	400	1.29e-51	173
MS.gene27285.t1	MTR_1g022105	48.408	157	76	2	68	224	170	321	5.17e-43	148
MS.gene27285.t1	MTR_1g008000	33.600	250	108	13	7	243	65	269	3.07e-24	98.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 54 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATCAACATCAATTTGAAAT+TGG	0.185453	1.1:-1334803	None:intergenic
AAATACAACACTCTTGTTAA+TGG	0.207720	1.1:+1334411	MS.gene27285:CDS
CTCCTGTCGTCTTGCACATT+TGG	0.210715	1.1:+1334625	MS.gene27285:CDS
ATAATCTAAGTACTAAATTA+TGG	0.229738	1.1:+1334334	MS.gene27285:CDS
TCCTGTCGTCTTGCACATTT+GGG	0.292237	1.1:+1334626	MS.gene27285:CDS
CAACTCCTTGGAGGAAAGTT+TGG	0.295354	1.1:+1334738	MS.gene27285:CDS
GTTTGGTGTCCTAGCTATCT+TGG	0.313901	1.1:-1334880	None:intergenic
ACAAGAGTGTTGTATTTAGA+AGG	0.319163	1.1:-1334405	None:intergenic
TCTTCTCTCGCAACTTTCAA+TGG	0.354491	1.1:+1334544	MS.gene27285:CDS
ATATATAATATATCCTAGTT+TGG	0.355918	1.1:-1334897	None:intergenic
TGACGTGTGATGATAGTTAA+AGG	0.366005	1.1:-1334378	None:intergenic
GGCAAAATGAGTTGCCTTGA+TGG	0.369019	1.1:-1334782	None:intergenic
TTGTTAATGGCGTTTATCAT+TGG	0.379442	1.1:+1334424	MS.gene27285:CDS
GACTCATGCATGTATGGTTT+TGG	0.395961	1.1:+1334213	MS.gene27285:CDS
ATGTTTGGCCTTTGGAAGAA+TGG	0.411360	1.1:+1334711	MS.gene27285:CDS
GACAACGACTCATGCATGTA+TGG	0.419933	1.1:+1334207	MS.gene27285:CDS
AAAGGAGGAGGATGTTGAGT+AGG	0.426239	1.1:-1334360	None:intergenic
TGTTTCGTATGTTTGGCCTT+TGG	0.429988	1.1:+1334703	MS.gene27285:CDS
AAGGAGGAGGATGTTGAGTA+GGG	0.432055	1.1:-1334359	None:intergenic
CTTCTCTCGCAACTTTCAAT+GGG	0.434082	1.1:+1334545	MS.gene27285:CDS
GCTTTGAAGTTGATGTTGTC+GGG	0.441312	1.1:-1334246	None:intergenic
GGAAAATGTTGCCGAGATCA+AGG	0.444536	1.1:+1334566	MS.gene27285:CDS
TGAAAGTTGCGAGAGAAGAT+TGG	0.450386	1.1:-1334540	None:intergenic
AGCTTTGAAGTTGATGTTGT+CGG	0.460508	1.1:-1334247	None:intergenic
CGGTGAACCTCTCTAGTTGT+TGG	0.473040	1.1:-1334162	None:intergenic
GGTGAACCTCTCTAGTTGTT+GGG	0.479668	1.1:-1334161	None:intergenic
CGTCTTGCACATTTGGGCAA+TGG	0.495898	1.1:+1334632	MS.gene27285:CDS
GCCCAAATGTGCAAGACGAC+AGG	0.509229	1.1:-1334627	None:intergenic
CTTTGTCAACCAAGATAGCT+AGG	0.511992	1.1:+1334871	MS.gene27285:CDS
AAGAATGGCGATCAACTCCT+TGG	0.512877	1.1:+1334726	MS.gene27285:CDS
AGCAAGGGAACCCTTGATCT+CGG	0.527450	1.1:-1334577	None:intergenic
GTTGACAAAGGAGCTATGCT+TGG	0.537962	1.1:-1334858	None:intergenic
GTGATGATAGTTAAAGGAGG+AGG	0.543019	1.1:-1334372	None:intergenic
AACAAGTTTCACACACTCAG+AGG	0.544907	1.1:+1334516	MS.gene27285:CDS
GATAGCTAGGACACCAAACT+AGG	0.547828	1.1:+1334884	MS.gene27285:CDS
AATGGCGATCAACTCCTTGG+AGG	0.570585	1.1:+1334729	MS.gene27285:CDS
GTTGATCGCCATTCTTCCAA+AGG	0.574741	1.1:-1334719	None:intergenic
TCAATTATTGTTCTGTGACA+AGG	0.579061	1.1:+1334283	MS.gene27285:CDS
GAAAATGTTGCCGAGATCAA+GGG	0.586335	1.1:+1334567	MS.gene27285:CDS
CTAGCTATCTTGGTTGACAA+AGG	0.595687	1.1:-1334870	None:intergenic
ACATGCATGAGTCGTTGTCA+AGG	0.608520	1.1:-1334203	None:intergenic
CTCACATCATATGACCATCA+AGG	0.608996	1.1:+1334768	MS.gene27285:CDS
GGGCAATGGATCAAAGTGGA+TGG	0.609401	1.1:+1334646	MS.gene27285:CDS
CGTGTGATGATAGTTAAAGG+AGG	0.609950	1.1:-1334375	None:intergenic
GATTCTGCACAACATAAGCA+AGG	0.613750	1.1:-1334593	None:intergenic
TCGTTGTCAAGGCAGAGACT+CGG	0.619869	1.1:-1334192	None:intergenic
TGATGGTCATATGATGTGAG+TGG	0.624286	1.1:-1334765	None:intergenic
ACAAGTTTCACACACTCAGA+GGG	0.630363	1.1:+1334517	MS.gene27285:CDS
GGCAGAGACTCGGAGGAACA+CGG	0.631574	1.1:-1334182	None:intergenic
GTGGAACCCAACAACTAGAG+AGG	0.665628	1.1:+1334155	MS.gene27285:CDS
ATTTGGGCAATGGATCAAAG+TGG	0.667392	1.1:+1334642	MS.gene27285:CDS
ATTCTGCACAACATAAGCAA+GGG	0.674457	1.1:-1334592	None:intergenic
TTGTCAAGGCAGAGACTCGG+AGG	0.701974	1.1:-1334189	None:intergenic
AGGAGGAGGATGTTGAGTAG+GGG	0.705269	1.1:-1334358	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAATCTAAGTACTAAATTA+TGG	+	chr1.1:1334334-1334353	MS.gene27285:CDS	15.0%
!!!	GAAACATTATATTTCATTTT+CGG	+	chr1.1:1334305-1334324	MS.gene27285:CDS	15.0%
!!!	TTGATGATAATGAATATTTT+TGG	+	chr1.1:1334820-1334839	MS.gene27285:CDS	15.0%
!	AAATACAACACTCTTGTTAA+TGG	+	chr1.1:1334411-1334430	MS.gene27285:CDS	25.0%
!	ATACGAAACATTGAAAAAAC+TGG	-	chr1.1:1334693-1334712	None:intergenic	25.0%
!	TACGAAACATTGAAAAAACT+GGG	-	chr1.1:1334692-1334711	None:intergenic	25.0%
!!	CATCAACATCAATTTGAAAT+TGG	-	chr1.1:1334806-1334825	None:intergenic	25.0%
!!	TTTTCAATGTTTCGTATGTT+TGG	+	chr1.1:1334696-1334715	MS.gene27285:CDS	25.0%
	TCAATTATTGTTCTGTGACA+AGG	+	chr1.1:1334283-1334302	MS.gene27285:CDS	30.0%
	TGGAGAAAAATGTACAACAT+TGG	+	chr1.1:1334666-1334685	MS.gene27285:CDS	30.0%
	TTGTTAATGGCGTTTATCAT+TGG	+	chr1.1:1334424-1334443	MS.gene27285:CDS	30.0%
!!	ACAAGAGTGTTGTATTTAGA+AGG	-	chr1.1:1334408-1334427	None:intergenic	30.0%
	ATTCTGCACAACATAAGCAA+GGG	-	chr1.1:1334595-1334614	None:intergenic	35.0%
	TGACGTGTGATGATAGTTAA+AGG	-	chr1.1:1334381-1334400	None:intergenic	35.0%
!!	AGCTTTGAAGTTGATGTTGT+CGG	-	chr1.1:1334250-1334269	None:intergenic	35.0%
	AACAAGTTTCACACACTCAG+AGG	+	chr1.1:1334516-1334535	MS.gene27285:CDS	40.0%
	ACAAGTTTCACACACTCAGA+GGG	+	chr1.1:1334517-1334536	MS.gene27285:CDS	40.0%
	ATTTGGGCAATGGATCAAAG+TGG	+	chr1.1:1334642-1334661	MS.gene27285:CDS	40.0%
	CGTGTGATGATAGTTAAAGG+AGG	-	chr1.1:1334378-1334397	None:intergenic	40.0%
	CTAGCTATCTTGGTTGACAA+AGG	-	chr1.1:1334873-1334892	None:intergenic	40.0%
	CTCACATCATATGACCATCA+AGG	+	chr1.1:1334768-1334787	MS.gene27285:CDS	40.0%
	CTTCTCTCGCAACTTTCAAT+GGG	+	chr1.1:1334545-1334564	MS.gene27285:CDS	40.0%
	CTTTGTCAACCAAGATAGCT+AGG	+	chr1.1:1334871-1334890	MS.gene27285:CDS	40.0%
	GAAAATGTTGCCGAGATCAA+GGG	+	chr1.1:1334567-1334586	MS.gene27285:CDS	40.0%
	GATTCTGCACAACATAAGCA+AGG	-	chr1.1:1334596-1334615	None:intergenic	40.0%
	GTGATGATAGTTAAAGGAGG+AGG	-	chr1.1:1334375-1334394	None:intergenic	40.0%
	TCTTCTCTCGCAACTTTCAA+TGG	+	chr1.1:1334544-1334563	MS.gene27285:CDS	40.0%
	TGAAAGTTGCGAGAGAAGAT+TGG	-	chr1.1:1334543-1334562	None:intergenic	40.0%
	TGATGGTCATATGATGTGAG+TGG	-	chr1.1:1334768-1334787	None:intergenic	40.0%
!	ATGTTTGGCCTTTGGAAGAA+TGG	+	chr1.1:1334711-1334730	MS.gene27285:CDS	40.0%
!	GACTCATGCATGTATGGTTT+TGG	+	chr1.1:1334213-1334232	MS.gene27285:CDS	40.0%
!	GTTTTCCAAACTTTCCTCCA+AGG	-	chr1.1:1334746-1334765	None:intergenic	40.0%
!	TGTTTCGTATGTTTGGCCTT+TGG	+	chr1.1:1334703-1334722	MS.gene27285:CDS	40.0%
!!	GCTTTGAAGTTGATGTTGTC+GGG	-	chr1.1:1334249-1334268	None:intergenic	40.0%
	AAAGGAGGAGGATGTTGAGT+AGG	-	chr1.1:1334363-1334382	None:intergenic	45.0%
	AAGAATGGCGATCAACTCCT+TGG	+	chr1.1:1334726-1334745	MS.gene27285:CDS	45.0%
	AAGGAGGAGGATGTTGAGTA+GGG	-	chr1.1:1334362-1334381	None:intergenic	45.0%
	ACATGCATGAGTCGTTGTCA+AGG	-	chr1.1:1334206-1334225	None:intergenic	45.0%
	CAACTCCTTGGAGGAAAGTT+TGG	+	chr1.1:1334738-1334757	MS.gene27285:CDS	45.0%
	GACAACGACTCATGCATGTA+TGG	+	chr1.1:1334207-1334226	MS.gene27285:CDS	45.0%
	GGAAAATGTTGCCGAGATCA+AGG	+	chr1.1:1334566-1334585	MS.gene27285:CDS	45.0%
	GGCAAAATGAGTTGCCTTGA+TGG	-	chr1.1:1334785-1334804	None:intergenic	45.0%
	GGTGAACCTCTCTAGTTGTT+GGG	-	chr1.1:1334164-1334183	None:intergenic	45.0%
	GTTGACAAAGGAGCTATGCT+TGG	-	chr1.1:1334861-1334880	None:intergenic	45.0%
	GTTGATCGCCATTCTTCCAA+AGG	-	chr1.1:1334722-1334741	None:intergenic	45.0%
	TCCTGTCGTCTTGCACATTT+GGG	+	chr1.1:1334626-1334645	MS.gene27285:CDS	45.0%
!!	GTTTGGTGTCCTAGCTATCT+TGG	-	chr1.1:1334883-1334902	None:intergenic	45.0%
	AATGGCGATCAACTCCTTGG+AGG	+	chr1.1:1334729-1334748	MS.gene27285:CDS	50.0%
	AGCAAGGGAACCCTTGATCT+CGG	-	chr1.1:1334580-1334599	None:intergenic	50.0%
	AGGAGGAGGATGTTGAGTAG+GGG	-	chr1.1:1334361-1334380	None:intergenic	50.0%
	CGGTGAACCTCTCTAGTTGT+TGG	-	chr1.1:1334165-1334184	None:intergenic	50.0%
	CGTCTTGCACATTTGGGCAA+TGG	+	chr1.1:1334632-1334651	MS.gene27285:CDS	50.0%
	CTCCTGTCGTCTTGCACATT+TGG	+	chr1.1:1334625-1334644	MS.gene27285:CDS	50.0%
	GGGCAATGGATCAAAGTGGA+TGG	+	chr1.1:1334646-1334665	MS.gene27285:CDS	50.0%
	GTGGAACCCAACAACTAGAG+AGG	+	chr1.1:1334155-1334174	MS.gene27285:CDS	50.0%
	TCGTTGTCAAGGCAGAGACT+CGG	-	chr1.1:1334195-1334214	None:intergenic	50.0%
	GCCCAAATGTGCAAGACGAC+AGG	-	chr1.1:1334630-1334649	None:intergenic	55.0%
	TTGTCAAGGCAGAGACTCGG+AGG	-	chr1.1:1334192-1334211	None:intergenic	55.0%
	GGCAGAGACTCGGAGGAACA+CGG	-	chr1.1:1334185-1334204	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	1334153	1334905	1334153	ID=MS.gene27285
chr1.1	mRNA	1334153	1334905	1334153	ID=MS.gene27285.t1;Parent=MS.gene27285
chr1.1	exon	1334153	1334905	1334153	ID=MS.gene27285.t1.exon1;Parent=MS.gene27285.t1
chr1.1	CDS	1334153	1334905	1334153	ID=cds.MS.gene27285.t1;Parent=MS.gene27285.t1

Gene Sequence

>MS.gene27285

ATGTGGAACCCAACAACTAGAGAGGTTCACCGTGTTCCTCCGAGTCTCTGCCTTGACAACGACTCATGCATGTATGGTTTTGGTGCTGATGATCCCGACAACATCAACTTCAAAGCTGTGAAACTTCATATCAATTATTGTTCTGTGACAAGGAAACATTATATTTCATTTTCGGAAGTTTATAATCTAAGTACTAAATTATGGACCCCTACTCAACATCCTCCTCCTTTAACTATCATCACACGTCAATGTCCTTCTAAATACAACACTCTTGTTAATGGCGTTTATCATTGGATTACAGAGAATACATACTACTATCACAATGATTATGCTGCAAATATTCTATGTTTTGACTTTCACAGCAACAAGTTTCACACACTCAGAGGGCCAATCTTCTCTCGCAACTTTCAATGGGAAAATGTTGCCGAGATCAAGGGTTCCCTTGCTTATGTTGTGCAGAATCACTTCAATGCTCCTGTCGTCTTGCACATTTGGGCAATGGATCAAAGTGGATGGAGAAAAATGTACAACATTGGCCCAGTTTTTTCAATGTTTCGTATGTTTGGCCTTTGGAAGAATGGCGATCAACTCCTTGGAGGAAAGTTTGGAAAACCACTCACATCATATGACCATCAAGGCAACTCATTTTGCCAATTTCAAATTGATGTTGATGATAATGAATATTTTTGGATTCATGAGTATGTGCCAAGCATAGCTCCTTTGTCAACCAAGATAGCTAGGACACCAAACTAG

Protein sequence

>MS.gene27285.t1

MWNPTTREVHRVPPSLCLDNDSCMYGFGADDPDNINFKAVKLHINYCSVTRKHYISFSEVYNLSTKLWTPTQHPPPLTIITRQCPSKYNTLVNGVYHWITENTYYYHNDYAANILCFDFHSNKFHTLRGPIFSRNFQWENVAEIKGSLAYVVQNHFNAPVVLHIWAMDQSGWRKMYNIGPVFSMFRMFGLWKNGDQLLGGKFGKPLTSYDHQGNSFCQFQIDVDDNEYFWIHEYVPSIAPLSTKIARTPN