AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69307


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69307.t1 MTR_4g109280 69.399 183 51 3 2 182 180 359 1.64e-84 253
MS.gene69307.t1 MTR_6g060650 67.778 180 55 2 2 181 196 372 4.55e-82 248
MS.gene69307.t1 MTR_3g103020 65.054 186 57 4 2 182 196 378 2.29e-77 236
MS.gene69307.t1 MTR_4g117720 65.608 189 53 7 2 182 194 378 2.29e-74 228
MS.gene69307.t1 MTR_4g109270 62.500 184 64 3 2 182 183 364 5.49e-71 219
MS.gene69307.t1 MTR_3g034180 61.290 186 63 3 2 181 306 488 4.10e-70 220
MS.gene69307.t1 MTR_3g034130 61.272 173 59 2 2 168 192 362 1.47e-67 210
MS.gene69307.t1 MTR_4g117850 63.784 185 58 6 1 179 195 376 5.95e-67 209
MS.gene69307.t1 MTR_4g118380 64.596 161 49 2 2 155 198 357 5.38e-65 203
MS.gene69307.t1 MTR_4g116320 60.326 184 62 6 1 179 78 255 2.65e-64 198
MS.gene69307.t1 MTR_3g034160 59.677 186 66 3 2 181 192 374 3.69e-63 199
MS.gene69307.t1 MTR_4g116330 58.919 185 69 4 2 182 199 380 1.45e-62 198
MS.gene69307.t1 MTR_4g094365 58.152 184 71 4 2 182 184 364 2.59e-62 197
MS.gene69307.t1 MTR_4g023130 58.470 183 66 5 2 181 137 312 2.86e-61 192
MS.gene69307.t1 MTR_8g040935 56.915 188 73 4 1 182 23 208 1.41e-60 187
MS.gene69307.t1 MTR_4g021785 55.978 184 74 4 1 182 177 355 5.25e-60 191
MS.gene69307.t1 MTR_4g122850 56.989 186 72 6 2 182 182 364 7.91e-60 191
MS.gene69307.t1 MTR_4g023080 56.831 183 69 5 2 181 178 353 5.05e-59 188
MS.gene69307.t1 MTR_4g118400 55.429 175 63 2 1 175 134 293 1.88e-58 185
MS.gene69307.t1 MTR_4g117780 55.435 184 79 2 2 182 184 367 1.95e-56 182
MS.gene69307.t1 MTR_8g433030 58.242 182 63 8 2 177 163 337 4.28e-56 180
MS.gene69307.t1 MTR_8g032390 58.242 182 63 8 2 177 196 370 1.51e-55 179
MS.gene69307.t1 MTR_4g117770 54.891 184 80 2 2 182 184 367 1.01e-54 177
MS.gene69307.t1 MTR_4g117810 54.891 184 80 2 2 182 184 367 1.01e-54 177
MS.gene69307.t1 MTR_4g023070 51.282 195 74 6 2 181 181 369 5.26e-54 176
MS.gene69307.t1 MTR_6g463430 51.913 183 80 3 2 182 26 202 1.83e-53 169
MS.gene69307.t1 MTR_4g023090 50.761 197 75 6 2 181 194 385 4.82e-53 173
MS.gene69307.t1 MTR_4g023380 50.242 207 71 6 2 182 179 379 1.84e-52 172
MS.gene69307.t1 MTR_1g055175 53.723 188 75 8 2 182 19 201 2.62e-52 166
MS.gene69307.t1 MTR_5g097210 56.044 182 73 5 1 179 183 360 2.76e-52 171
MS.gene69307.t1 MTR_4g023120 50.000 196 77 6 2 182 181 370 6.60e-52 170
MS.gene69307.t1 MTR_1g055195 56.442 163 69 1 20 182 318 478 7.34e-52 172
MS.gene69307.t1 MTR_4g023190 54.301 186 70 7 2 180 180 357 1.34e-51 169
MS.gene69307.t1 MTR_8g433040 54.054 185 66 7 2 182 195 364 2.24e-51 169
MS.gene69307.t1 MTR_8g032380 54.054 185 66 7 2 182 195 364 2.24e-51 169
MS.gene69307.t1 MTR_5g070130 51.832 191 81 5 2 182 188 377 8.07e-51 168
MS.gene69307.t1 MTR_1g055165 56.886 167 63 6 20 182 157 318 4.00e-49 161
MS.gene69307.t1 MTR_4g118360 64.286 126 41 2 59 182 3 126 2.76e-48 153
MS.gene69307.t1 MTR_5g070080 52.432 185 80 6 2 182 206 386 8.52e-48 160
MS.gene69307.t1 MTR_4g023150 51.613 186 76 7 2 180 180 358 1.56e-46 156
MS.gene69307.t1 MTR_4g023200 50.000 186 79 7 2 180 180 358 1.66e-46 156
MS.gene69307.t1 MTR_4g118370 48.352 182 70 5 1 179 97 257 5.15e-46 152
MS.gene69307.t1 MTR_4g117750 50.000 190 83 5 1 181 172 358 1.38e-45 156
MS.gene69307.t1 MTR_4g117750 61.468 109 40 1 74 180 373 481 8.61e-37 133
MS.gene69307.t1 MTR_8g102520 46.486 185 93 4 2 182 140 322 2.10e-45 152
MS.gene69307.t1 MTR_7g079400 43.915 189 96 4 2 182 151 337 1.50e-43 147
MS.gene69307.t1 MTR_5g070088 46.369 179 93 2 1 177 203 380 2.11e-43 148
MS.gene69307.t1 MTR_1g055135 51.064 188 76 7 5 182 194 375 2.32e-43 148
MS.gene69307.t1 MTR_4g117870 47.253 182 57 5 2 182 124 267 4.50e-43 144
MS.gene69307.t1 MTR_4g116330 48.108 185 50 4 2 182 199 341 1.88e-41 142
MS.gene69307.t1 MTR_4g023440 44.751 181 59 3 2 182 193 332 5.44e-41 141
MS.gene69307.t1 MTR_7g079370 44.944 178 91 5 2 175 175 349 8.98e-38 133
MS.gene69307.t1 MTR_1g055250 47.159 176 77 10 1 169 87 253 9.41e-38 132
MS.gene69307.t1 MTR_5g023000 65.591 93 32 0 90 182 7 99 1.34e-37 125
MS.gene69307.t1 MTR_4g117530 42.541 181 70 3 2 182 107 253 1.67e-37 130
MS.gene69307.t1 MTR_8g098830 44.805 154 79 4 2 152 45 195 2.66e-37 129
MS.gene69307.t1 MTR_7g079450 47.183 142 68 3 42 182 2 137 1.16e-33 116
MS.gene69307.t1 MTR_4g122860 43.085 188 71 7 2 182 162 320 4.37e-33 120
MS.gene69307.t1 MTR_7g079420 40.107 187 97 7 5 182 964 1144 1.83e-32 122
MS.gene69307.t1 MTR_2g048580 36.364 187 101 7 2 182 168 342 2.37e-30 113
MS.gene69307.t1 MTR_4g114700 36.667 180 62 4 1 176 90 221 1.66e-29 108
MS.gene69307.t1 MTR_4g121580 42.222 180 67 8 1 175 195 342 1.53e-28 109
MS.gene69307.t1 MTR_4g117430 57.647 85 32 2 2 86 75 155 3.39e-22 87.8
MS.gene69307.t1 MTR_8g013010 59.756 82 26 3 2 83 235 309 2.35e-21 89.0
MS.gene69307.t1 MTR_7g117720 51.515 99 43 3 82 175 172 270 2.81e-21 88.2
MS.gene69307.t1 MTR_4g023430 53.659 82 35 1 104 182 141 222 4.48e-21 86.7
MS.gene69307.t1 MTR_4g122840 41.304 138 41 5 48 182 164 264 1.55e-17 78.2
MS.gene69307.t1 MTR_8g013005 59.322 59 24 0 124 182 1 59 3.45e-17 72.0
MS.gene69307.t1 MTR_4g109290 57.895 57 24 0 126 182 132 188 1.84e-14 68.6
MS.gene69307.t1 MTR_1g055145 48.000 75 35 2 25 98 131 202 9.38e-12 62.4
MS.gene69307.t1 MTR_3g010970 27.072 181 110 6 2 174 225 391 1.85e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 47 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATGACTTGTGGGTATT+TGG 0.144797 8.2:+16565118 MS.gene69307:CDS
TATTCACTAGCGTTCAAATA+AGG 0.220938 8.2:-16564938 None:intergenic
GGAGCAGGGTTCCTCTTTAA+TGG 0.253120 8.2:+16564968 MS.gene69307:CDS
GAGCAGGGTTCCTCTTTAAT+GGG 0.265091 8.2:+16564969 MS.gene69307:CDS
TTGGAGAATTTCTCAGTCTA+TGG 0.267319 8.2:+16565137 MS.gene69307:CDS
ACGAAGCTACAGTTGAAATA+TGG 0.272308 8.2:+16565167 MS.gene69307:CDS
ACAAAATGTGGTGATATTAT+TGG 0.310598 8.2:+16565286 MS.gene69307:CDS
ACTCTTGTCCTTCCTATTGA+TGG 0.321860 8.2:+16565229 MS.gene69307:CDS
TCTTTAATGGGGCTATTCAT+TGG 0.331439 8.2:+16564981 MS.gene69307:CDS
ACCCACAAGTCATAATAATT+AGG 0.339487 8.2:-16565112 None:intergenic
ATTCACTAGCGTTCAAATAA+GGG 0.371657 8.2:-16564937 None:intergenic
TCCACATGGATTGTTGAAAT+AGG 0.371891 8.2:-16565371 None:intergenic
TTGGCGAAGTACGATGAAAA+TGG 0.379685 8.2:+16565328 MS.gene69307:CDS
TAATGGGGCTATTCATTGGT+TGG 0.427301 8.2:+16564985 MS.gene69307:CDS
AGATAATATGTGGAAAGAGC+TGG 0.433933 8.2:+16564901 None:intergenic
ATTAAAGAGGAACCCTGCTC+CGG 0.440956 8.2:-16564966 None:intergenic
TGAGTTGGAATGCCATCAAT+AGG 0.444967 8.2:-16565241 None:intergenic
ATTGGAACAGATGGTGGTAA+TGG 0.447081 8.2:+16565304 MS.gene69307:CDS
ATCCTAATTATTATGACTTG+TGG 0.450172 8.2:+16565110 MS.gene69307:CDS
TCCTAATTATTATGACTTGT+GGG 0.453742 8.2:+16565111 MS.gene69307:CDS
CTCAGTATACATGGCCGCTC+GGG 0.457520 8.2:-16565395 None:intergenic
CGAAGCTACAGTTGAAATAT+GGG 0.462892 8.2:+16565168 MS.gene69307:CDS
TGGAGAATTTCTCAGTCTAT+GGG 0.490268 8.2:+16565138 MS.gene69307:CDS
AGTCATAATAATTAGGATCA+TGG 0.492245 8.2:-16565105 None:intergenic
AATACAAAGTGCATTCGTCT+TGG 0.494278 8.2:+16565200 MS.gene69307:CDS
AGTGAATATAATCCCGGAGC+AGG 0.497997 8.2:+16564953 MS.gene69307:CDS
ATTTCTCAGTCTATGGGCTA+TGG 0.507502 8.2:+16565144 MS.gene69307:CDS
AATGAATAGCCCCATTAAAG+AGG 0.526680 8.2:-16564979 None:intergenic
AACGCTAGTGAATATAATCC+CGG 0.527662 8.2:+16564947 MS.gene69307:CDS
ACTCAGTATACATGGCCGCT+CGG 0.535525 8.2:-16565396 None:intergenic
TTAAAGAGGAACCCTGCTCC+GGG 0.544713 8.2:-16564965 None:intergenic
AATGGGTAGAAGTACTGAGT+TGG 0.583626 8.2:-16565256 None:intergenic
TAATATGTGGAAAGAGCTGG+AGG 0.586191 8.2:+16564904 None:intergenic
TAGCAGAGACTCAGTATACA+TGG 0.587628 8.2:-16565404 None:intergenic
GGTGATATTATTGGAACAGA+TGG 0.588464 8.2:+16565295 MS.gene69307:CDS
GAGTCTCTGCTATCACTCCT+CGG 0.588999 8.2:+16565415 MS.gene69307:CDS
GATATTATTGGAACAGATGG+TGG 0.593108 8.2:+16565298 MS.gene69307:CDS
AACAGATGGTGGTAATGGAT+TGG 0.598103 8.2:+16565309 MS.gene69307:CDS
AGCAGGGTTCCTCTTTAATG+GGG 0.611689 8.2:+16564970 MS.gene69307:CDS
TTGGAATGCCATCAATAGGA+AGG 0.614361 8.2:-16565237 None:intergenic
TCCTATTTCAACAATCCATG+TGG 0.619417 8.2:+16565370 MS.gene69307:CDS
GTGAATATAATCCCGGAGCA+GGG 0.646070 8.2:+16564954 MS.gene69307:CDS
ATGGCCGCTCGGGATCCACA+TGG 0.650158 8.2:-16565385 None:intergenic
CCATTGTGCTGTACAAAATG+TGG 0.678555 8.2:+16565274 MS.gene69307:CDS
CAATCCATGTGGATCCCGAG+CGG 0.712280 8.2:+16565381 MS.gene69307:CDS
AAGCTTGCTCATTGTCACCG+AGG 0.712518 8.2:-16565432 None:intergenic
AATATGTGGAAAGAGCTGGA+GGG 0.747768 8.2:+16564905 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCTTTTCTATTAAATCAA+AGG - chr8.2:16565047-16565066 None:intergenic 15.0%
! ACAAAATGTGGTGATATTAT+TGG + chr8.2:16565286-16565305 MS.gene69307:CDS 25.0%
! AGTCATAATAATTAGGATCA+TGG - chr8.2:16565108-16565127 None:intergenic 25.0%
! ATCCTAATTATTATGACTTG+TGG + chr8.2:16565110-16565129 MS.gene69307:CDS 25.0%
! TGGTCAAAAATAAAATCATC+CGG - chr8.2:16565088-16565107 None:intergenic 25.0%
!! ACTTTTAGATATACATTTGC+CGG + chr8.2:16565066-16565085 MS.gene69307:CDS 25.0%
!! TCCTAATTATTATGACTTGT+GGG + chr8.2:16565111-16565130 MS.gene69307:CDS 25.0%
ACCCACAAGTCATAATAATT+AGG - chr8.2:16565115-16565134 None:intergenic 30.0%
ATTCACTAGCGTTCAAATAA+GGG - chr8.2:16564940-16564959 None:intergenic 30.0%
TATTCACTAGCGTTCAAATA+AGG - chr8.2:16564941-16564960 None:intergenic 30.0%
! TATTATGACTTGTGGGTATT+TGG + chr8.2:16565118-16565137 MS.gene69307:CDS 30.0%
AACGCTAGTGAATATAATCC+CGG + chr8.2:16564947-16564966 MS.gene69307:CDS 35.0%
AATACAAAGTGCATTCGTCT+TGG + chr8.2:16565200-16565219 MS.gene69307:CDS 35.0%
AATGAATAGCCCCATTAAAG+AGG - chr8.2:16564982-16565001 None:intergenic 35.0%
ACGAAGCTACAGTTGAAATA+TGG + chr8.2:16565167-16565186 MS.gene69307:CDS 35.0%
CGAAGCTACAGTTGAAATAT+GGG + chr8.2:16565168-16565187 MS.gene69307:CDS 35.0%
GATATTATTGGAACAGATGG+TGG + chr8.2:16565298-16565317 MS.gene69307:CDS 35.0%
GGTGATATTATTGGAACAGA+TGG + chr8.2:16565295-16565314 MS.gene69307:CDS 35.0%
TCCTATTTCAACAATCCATG+TGG + chr8.2:16565370-16565389 MS.gene69307:CDS 35.0%
TCTTTAATGGGGCTATTCAT+TGG + chr8.2:16564981-16565000 MS.gene69307:CDS 35.0%
TGGAGAATTTCTCAGTCTAT+GGG + chr8.2:16565138-16565157 MS.gene69307:CDS 35.0%
TTGGAGAATTTCTCAGTCTA+TGG + chr8.2:16565137-16565156 MS.gene69307:CDS 35.0%
! CACATTTTGTACAGCACAAT+GGG - chr8.2:16565276-16565295 None:intergenic 35.0%
! TCCACATGGATTGTTGAAAT+AGG - chr8.2:16565374-16565393 None:intergenic 35.0%
ACTCTTGTCCTTCCTATTGA+TGG + chr8.2:16565229-16565248 MS.gene69307:CDS 40.0%
CCATTGTGCTGTACAAAATG+TGG + chr8.2:16565274-16565293 MS.gene69307:CDS 40.0%
TAATGGGGCTATTCATTGGT+TGG + chr8.2:16564985-16565004 MS.gene69307:CDS 40.0%
TAGCAGAGACTCAGTATACA+TGG - chr8.2:16565407-16565426 None:intergenic 40.0%
TTGGAATGCCATCAATAGGA+AGG - chr8.2:16565240-16565259 None:intergenic 40.0%
TTGGCGAAGTACGATGAAAA+TGG + chr8.2:16565328-16565347 MS.gene69307:CDS 40.0%
! ATTGGAACAGATGGTGGTAA+TGG + chr8.2:16565304-16565323 MS.gene69307:CDS 40.0%
! ATTTCTCAGTCTATGGGCTA+TGG + chr8.2:16565144-16565163 MS.gene69307:CDS 40.0%
! CCACATTTTGTACAGCACAA+TGG - chr8.2:16565277-16565296 None:intergenic 40.0%
! TGAGTTGGAATGCCATCAAT+AGG - chr8.2:16565244-16565263 None:intergenic 40.0%
!! AACAGATGGTGGTAATGGAT+TGG + chr8.2:16565309-16565328 MS.gene69307:CDS 40.0%
!! AATGGGTAGAAGTACTGAGT+TGG - chr8.2:16565259-16565278 None:intergenic 40.0%
AGCAGGGTTCCTCTTTAATG+GGG + chr8.2:16564970-16564989 MS.gene69307:CDS 45.0%
AGTGAATATAATCCCGGAGC+AGG + chr8.2:16564953-16564972 MS.gene69307:CDS 45.0%
ATTAAAGAGGAACCCTGCTC+CGG - chr8.2:16564969-16564988 None:intergenic 45.0%
GAGCAGGGTTCCTCTTTAAT+GGG + chr8.2:16564969-16564988 MS.gene69307:CDS 45.0%
GTGAATATAATCCCGGAGCA+GGG + chr8.2:16564954-16564973 MS.gene69307:CDS 45.0%
ACTCAGTATACATGGCCGCT+CGG - chr8.2:16565399-16565418 None:intergenic 50.0%
GAGTCTCTGCTATCACTCCT+CGG + chr8.2:16565415-16565434 MS.gene69307:CDS 50.0%
GGAGCAGGGTTCCTCTTTAA+TGG + chr8.2:16564968-16564987 MS.gene69307:CDS 50.0%
TTAAAGAGGAACCCTGCTCC+GGG - chr8.2:16564968-16564987 None:intergenic 50.0%
! AAGCTTGCTCATTGTCACCG+AGG - chr8.2:16565435-16565454 None:intergenic 50.0%
CAATCCATGTGGATCCCGAG+CGG + chr8.2:16565381-16565400 MS.gene69307:CDS 55.0%
CTCAGTATACATGGCCGCTC+GGG - chr8.2:16565398-16565417 None:intergenic 55.0%
ATGGCCGCTCGGGATCCACA+TGG - chr8.2:16565388-16565407 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 16564908 16565456 16564908 ID=MS.gene69307
chr8.2 mRNA 16564908 16565456 16564908 ID=MS.gene69307.t1;Parent=MS.gene69307
chr8.2 exon 16564908 16565456 16564908 ID=MS.gene69307.t1.exon1;Parent=MS.gene69307.t1
chr8.2 CDS 16564908 16565456 16564908 ID=cds.MS.gene69307.t1;Parent=MS.gene69307.t1
Gene Sequence

>MS.gene69307

ATGTGGAAAGAGCTGGAGGGTACTCACTTCCCTTATTTGAACGCTAGTGAATATAATCCCGGAGCAGGGTTCCTCTTTAATGGGGCTATTCATTGGTTGGCTTTTAGTTGTGATCTACTATATGATGTTATTGTTGCCTTTGATTTAATAGAAAAGAAACTTTTAGATATACATTTGCCGGATGATTTTATTTTTGACCATGATCCTAATTATTATGACTTGTGGGTATTTGGAGAATTTCTCAGTCTATGGGCTATGGACGAAGCTACAGTTGAAATATGGGTTATGAAAGAATACAAAGTGCATTCGTCTTGGACTAAGACTCTTGTCCTTCCTATTGATGGCATTCCAACTCAGTACTTCTACCCATTGTGCTGTACAAAATGTGGTGATATTATTGGAACAGATGGTGGTAATGGATTGGCGAAGTACGATGAAAATGGAGAATTACTAGACGTTCATTCCTATTTCAACAATCCATGTGGATCCCGAGCGGCCATGTATACTGAGTCTCTGCTATCACTCCTCGGTGACAATGAGCAAGCTTAA

Protein sequence

>MS.gene69307.t1

MWKELEGTHFPYLNASEYNPGAGFLFNGAIHWLAFSCDLLYDVIVAFDLIEKKLLDIHLPDDFIFDHDPNYYDLWVFGEFLSLWAMDEATVEIWVMKEYKVHSSWTKTLVLPIDGIPTQYFYPLCCTKCGDIIGTDGGNGLAKYDENGELLDVHSYFNNPCGSRAAMYTESLLSLLGDNEQA