AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69309

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69309.t1 MTR_4g109270 57.182 369 106 9 1 322 1 364 2.32e-120 350
MS.gene69309.t1 MTR_3g034180 50.133 377 129 10 1 321 115 488 5.16e-102 308
MS.gene69309.t1 MTR_3g103020 50.954 367 129 10 4 322 15 378 3.10e-100 300
MS.gene69309.t1 MTR_3g034130 50.000 364 124 9 1 308 1 362 8.41e-100 298
MS.gene69309.t1 MTR_4g117850 51.791 363 118 11 7 319 21 376 2.89e-98 295
MS.gene69309.t1 MTR_3g034160 48.670 376 136 8 1 321 1 374 4.81e-97 291
MS.gene69309.t1 MTR_4g117770 48.361 366 142 7 2 322 4 367 4.25e-92 279
MS.gene69309.t1 MTR_4g117810 48.361 366 142 7 2 322 4 367 4.25e-92 279
MS.gene69309.t1 MTR_4g023080 49.724 362 132 12 1 321 1 353 2.52e-91 276
MS.gene69309.t1 MTR_4g117780 46.721 366 148 7 2 322 4 367 2.37e-89 271
MS.gene69309.t1 MTR_4g021785 49.036 363 136 11 1 322 1 355 2.17e-88 269
MS.gene69309.t1 MTR_4g116330 47.043 372 139 13 2 322 16 380 8.90e-88 268
MS.gene69309.t1 MTR_4g023090 45.026 382 143 13 2 321 9 385 1.42e-86 265
MS.gene69309.t1 MTR_4g109280 47.123 365 139 10 4 322 3 359 6.45e-86 263
MS.gene69309.t1 MTR_4g023130 50.303 330 137 13 1 321 1 312 1.18e-84 258
MS.gene69309.t1 MTR_6g060650 46.486 370 138 10 8 321 7 372 1.45e-81 252
MS.gene69309.t1 MTR_5g070080 44.737 380 143 10 8 322 9 386 2.00e-81 252
MS.gene69309.t1 MTR_4g122850 45.070 355 141 11 20 322 12 364 8.73e-81 250
MS.gene69309.t1 MTR_5g070130 45.000 380 148 15 1 322 1 377 2.99e-80 249
MS.gene69309.t1 MTR_4g094365 44.173 369 154 11 1 322 1 364 7.02e-79 244
MS.gene69309.t1 MTR_8g433040 44.687 367 132 11 8 322 17 364 5.97e-78 242
MS.gene69309.t1 MTR_8g032380 44.687 367 132 11 8 322 17 364 5.97e-78 242
MS.gene69309.t1 MTR_8g433030 48.276 319 136 8 8 317 39 337 1.69e-77 240
MS.gene69309.t1 MTR_4g023070 44.920 374 138 12 9 321 3 369 3.60e-77 241
MS.gene69309.t1 MTR_5g097210 44.693 358 143 11 9 318 9 359 2.04e-76 239
MS.gene69309.t1 MTR_8g032390 45.509 334 156 9 8 317 39 370 7.26e-76 237
MS.gene69309.t1 MTR_5g070088 42.636 387 149 11 1 319 1 382 4.30e-75 236
MS.gene69309.t1 MTR_4g023120 43.200 375 145 11 9 322 3 370 1.15e-74 234
MS.gene69309.t1 MTR_1g055135 44.324 370 147 15 7 322 11 375 1.27e-73 231
MS.gene69309.t1 MTR_4g023380 43.077 390 134 13 2 322 9 379 1.54e-73 231
MS.gene69309.t1 MTR_4g023190 45.378 357 143 12 8 320 9 357 6.44e-73 229
MS.gene69309.t1 MTR_4g023440 43.490 361 125 10 2 322 11 332 4.57e-72 226
MS.gene69309.t1 MTR_4g023200 44.767 344 135 12 23 320 24 358 2.77e-71 225
MS.gene69309.t1 MTR_4g117750 44.658 365 141 11 9 321 3 358 1.12e-70 227
MS.gene69309.t1 MTR_4g117750 55.963 109 43 3 217 320 373 481 2.10e-25 106
MS.gene69309.t1 MTR_4g117720 46.628 341 157 12 1 322 44 378 1.55e-70 224
MS.gene69309.t1 MTR_4g023150 45.125 359 142 12 8 320 9 358 1.64e-70 223
MS.gene69309.t1 MTR_4g118380 43.646 362 122 11 7 296 6 357 5.62e-70 222
MS.gene69309.t1 MTR_4g116330 42.276 369 122 15 2 322 16 341 8.80e-67 213
MS.gene69309.t1 MTR_1g055165 41.840 337 159 11 2 322 3 318 1.13e-64 207
MS.gene69309.t1 MTR_8g013010 47.388 268 81 8 8 226 53 309 2.75e-63 203
MS.gene69309.t1 MTR_8g040935 50.739 203 86 7 131 322 9 208 4.40e-54 176
MS.gene69309.t1 MTR_4g116320 49.083 218 91 6 117 319 43 255 1.97e-53 176
MS.gene69309.t1 MTR_4g117870 39.590 293 100 6 72 322 10 267 1.09e-51 172
MS.gene69309.t1 MTR_1g055195 46.637 223 103 8 111 322 261 478 5.08e-49 171
MS.gene69309.t1 MTR_4g117530 47.196 214 78 6 111 322 73 253 1.63e-48 163
MS.gene69309.t1 MTR_6g463430 48.718 195 83 5 137 322 16 202 8.85e-47 157
MS.gene69309.t1 MTR_2g048580 34.770 348 182 13 8 322 7 342 8.50e-46 159
MS.gene69309.t1 MTR_8g102520 35.988 339 170 12 8 322 7 322 1.63e-44 155
MS.gene69309.t1 MTR_4g121580 38.040 347 129 17 1 290 1 318 3.93e-44 155
MS.gene69309.t1 MTR_4g118370 42.534 221 96 7 111 319 56 257 3.08e-43 149
MS.gene69309.t1 MTR_4g118400 46.766 201 91 5 119 316 107 294 8.33e-43 149
MS.gene69309.t1 MTR_1g055175 43.523 193 102 4 137 322 9 201 1.58e-40 140
MS.gene69309.t1 MTR_7g079400 35.473 296 141 10 76 322 43 337 5.51e-39 140
MS.gene69309.t1 MTR_4g122860 34.535 333 131 13 2 322 63 320 1.32e-37 137
MS.gene69309.t1 MTR_7g079370 35.072 345 148 14 38 316 16 350 1.35e-37 137
MS.gene69309.t1 MTR_4g116340 40.541 222 98 8 2 192 29 247 8.70e-37 132
MS.gene69309.t1 MTR_4g117430 55.645 124 51 2 105 228 35 154 8.79e-36 127
MS.gene69309.t1 MTR_4g109290 56.643 143 39 6 1 128 1 135 1.01e-35 128
MS.gene69309.t1 MTR_4g109290 64.151 53 18 1 271 322 136 188 1.24e-11 63.2
MS.gene69309.t1 MTR_1g055250 39.423 208 113 7 112 309 49 253 7.38e-34 127
MS.gene69309.t1 MTR_5g023000 62.366 93 33 2 232 322 7 99 1.90e-30 111
MS.gene69309.t1 MTR_4g118360 53.043 115 49 3 212 322 13 126 1.38e-29 110
MS.gene69309.t1 MTR_6g034720 52.252 111 45 3 99 203 61 169 6.37e-27 105
MS.gene69309.t1 MTR_1g094210 27.500 360 193 12 7 314 55 398 9.01e-26 106
MS.gene69309.t1 MTR_2g021190 29.650 371 183 15 6 314 18 372 5.54e-25 103
MS.gene69309.t1 MTR_1g094210 27.811 338 176 12 7 292 55 376 5.57e-25 104
MS.gene69309.t1 MTR_4g114700 35.814 215 82 7 110 313 49 218 5.88e-25 101
MS.gene69309.t1 MTR_7g079420 34.375 224 107 8 110 322 950 1144 1.79e-23 101
MS.gene69309.t1 MTR_4g108820 31.365 271 164 9 7 265 40 300 2.68e-23 98.2
MS.gene69309.t1 MTR_8g098830 40.361 166 83 7 137 292 35 194 4.52e-23 95.9
MS.gene69309.t1 MTR_1g097330 28.660 321 175 14 1 274 8 321 1.67e-21 94.7
MS.gene69309.t1 MTR_7g079450 41.549 142 71 5 187 322 2 137 1.68e-21 89.0
MS.gene69309.t1 MTR_3g083640 27.049 366 195 16 6 314 52 402 3.67e-21 93.6
MS.gene69309.t1 MTR_5g022420 25.613 367 213 14 2 314 17 377 9.82e-21 92.0
MS.gene69309.t1 MTR_4g023430 57.647 85 33 2 8 90 40 123 1.31e-20 89.0
MS.gene69309.t1 MTR_4g023430 50.617 81 35 3 247 322 142 222 2.05e-13 68.9
MS.gene69309.t1 MTR_2g078900 27.190 331 168 12 2 278 23 334 3.98e-20 90.1
MS.gene69309.t1 MTR_4g133170 26.786 336 189 16 6 290 1 330 1.25e-19 89.0
MS.gene69309.t1 MTR_1g097490 24.650 357 205 12 8 314 48 390 1.63e-19 88.6
MS.gene69309.t1 MTR_3g083660 28.846 260 112 8 3 255 19 212 8.15e-19 85.1
MS.gene69309.t1 MTR_3g036335 26.062 353 195 15 1 302 33 370 1.09e-18 86.3
MS.gene69309.t1 MTR_2g021150 27.052 329 169 15 6 274 11 328 1.58e-18 85.5
MS.gene69309.t1 MTR_7g117720 33.030 330 147 15 2 317 3 272 2.29e-18 84.0
MS.gene69309.t1 MTR_1g058190 26.667 315 196 10 4 291 30 336 2.52e-18 85.1
MS.gene69309.t1 MTR_2g426020 27.320 388 197 17 8 319 5 383 3.75e-18 84.7
MS.gene69309.t1 MTR_1g097560 24.438 356 203 13 8 314 35 373 5.63e-18 84.0
MS.gene69309.t1 MTR_1g055145 54.762 84 36 2 23 105 47 129 5.15e-17 80.1
MS.gene69309.t1 MTR_1g114700 25.469 373 203 11 6 314 26 387 6.12e-17 81.3
MS.gene69309.t1 MTR_3g006670 28.896 308 156 14 6 261 46 342 7.13e-17 81.3
MS.gene69309.t1 MTR_2g024110 25.330 379 203 18 9 319 4 370 7.90e-17 80.9
MS.gene69309.t1 MTR_1g115160 25.240 313 176 10 8 278 21 317 1.34e-16 80.1
MS.gene69309.t1 MTR_0128s0070 25.964 389 191 16 8 313 21 395 1.74e-16 79.7
MS.gene69309.t1 MTR_5g008530 69.492 59 15 2 8 66 17 72 2.20e-16 73.2
MS.gene69309.t1 MTR_3g014570 27.913 369 198 15 2 314 22 378 3.12e-16 79.0
MS.gene69309.t1 MTR_8g464770 24.339 378 206 15 8 316 11 377 7.79e-16 77.8
MS.gene69309.t1 MTR_1g068835 27.011 348 177 16 9 294 4 336 1.32e-15 77.0
MS.gene69309.t1 MTR_3g011020 27.304 293 162 9 6 256 25 308 2.79e-15 76.3
MS.gene69309.t1 MTR_3g023930 28.472 288 148 13 6 254 31 299 3.81e-15 75.9
MS.gene69309.t1 MTR_7g055830 27.855 359 197 16 9 317 13 359 4.30e-15 75.5
MS.gene69309.t1 MTR_2g021050 28.826 281 152 11 9 247 5 279 5.22e-15 75.5
MS.gene69309.t1 MTR_3g009240 27.178 287 157 8 6 248 33 311 7.81e-15 75.1
MS.gene69309.t1 MTR_0128s0020 24.227 388 198 15 9 313 18 392 2.93e-14 73.2
MS.gene69309.t1 MTR_1g054725 25.594 379 192 17 6 314 17 375 4.93e-14 72.4
MS.gene69309.t1 MTR_8g013005 55.932 59 25 1 265 322 1 59 5.30e-14 66.2
MS.gene69309.t1 MTR_5g022850 23.343 347 225 11 8 320 15 354 4.67e-13 69.3
MS.gene69309.t1 MTR_7g066030 22.727 396 204 11 9 315 21 403 1.26e-12 68.6
MS.gene69309.t1 MTR_3g010870 26.216 370 193 17 6 314 34 384 1.59e-12 68.2
MS.gene69309.t1 MTR_1g055055 25.368 272 151 10 9 237 26 288 2.14e-12 67.4
MS.gene69309.t1 MTR_3g030320 28.571 161 101 6 112 266 90 242 3.79e-12 66.2
MS.gene69309.t1 MTR_3g096250 26.727 333 189 14 6 314 44 345 4.49e-12 66.6
MS.gene69309.t1 MTR_5g023570 57.627 59 21 2 1 56 26 83 5.62e-12 63.2
MS.gene69309.t1 MTR_2g078870 26.941 219 133 8 112 314 100 307 1.50e-11 64.7
MS.gene69309.t1 MTR_6g013270 46.269 67 36 0 3 69 23 89 3.64e-11 63.9
MS.gene69309.t1 MTR_2g024100 30.570 193 108 9 110 284 153 337 8.84e-11 62.8
MS.gene69309.t1 MTR_7g022670 24.217 351 198 13 9 314 23 350 9.85e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69309.t1 AT3G23880 29.221 308 162 14 7 265 11 311 1.08e-17 83.2
MS.gene69309.t1 AT3G06240 29.717 212 134 5 112 314 214 419 4.07e-16 79.0
MS.gene69309.t1 AT2G43445 22.590 363 215 14 2 316 6 350 2.69e-14 73.6

Find 70 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGAATATTTCTATGGTTT+TGG 0.184892 8.2:+16542066 MS.gene69309:CDS
TATTCAGATCTTGAAATTAA+AGG 0.189024 8.2:+16541925 MS.gene69309:CDS
AAAGCTGGGTTGTTCTTTAA+TGG 0.210542 8.2:+16542240 MS.gene69309:CDS
GATTCTGGTTTGTGGGTATT+TGG 0.239819 8.2:+16542384 MS.gene69309:CDS
AAACATGAACCTTACGATTC+TGG 0.242156 8.2:+16542369 MS.gene69309:CDS
TGGGTATTTGGAGAATTTCT+TGG 0.272087 8.2:+16542396 MS.gene69309:CDS
CCGGTGACCTTAGTAGGATT+TGG 0.292066 8.2:-16541678 None:intergenic
GGTTCATGTTTAAGCTTATA+TGG 0.295734 8.2:-16542357 None:intergenic
AAGCTGGGTTGTTCTTTAAT+GGG 0.303358 8.2:+16542241 MS.gene69309:CDS
CTCGTCAACAGATGATTATT+TGG 0.303514 8.2:+16542095 MS.gene69309:CDS
AAGGAATGTGAATATTTCTA+TGG 0.304244 8.2:+16542060 MS.gene69309:CDS
GAGTCTCTGCTTTCACTTCC+CGG 0.311578 8.2:+16542672 MS.gene69309:CDS
ATCGTGGTGCAGCTGAAATA+TGG 0.311591 8.2:+16542430 MS.gene69309:CDS
TAATACATGGAAACAAATTC+AGG 0.328938 8.2:+16542176 MS.gene69309:CDS
AGGTCCATATGATTCGTTAT+AGG 0.333106 8.2:-16542631 None:intergenic
TTGGAGAATTTCTTGGTTTG+TGG 0.336914 8.2:+16542403 MS.gene69309:CDS
TCTTTAATGGGGCTATTCAT+TGG 0.349624 8.2:+16542253 MS.gene69309:CDS
TGGAGAATTTCTTGGTTTGT+GGG 0.354642 8.2:+16542404 MS.gene69309:CDS
ATTAAAGAACAACCCAGCTT+TGG 0.366565 8.2:-16542238 None:intergenic
TTATACTTCACCAATCTGTT+AGG 0.384638 8.2:-16542579 None:intergenic
TCTGTATACACGGCCACTTC+AGG 0.389246 8.2:-16542651 None:intergenic
TTAAAGAACAACCCAGCTTT+GGG 0.389953 8.2:-16542237 None:intergenic
ACTTCACCGGTGACCTTAGT+AGG 0.396149 8.2:-16541684 None:intergenic
ACCTTACGATTCTGGTTTGT+GGG 0.398636 8.2:+16542377 MS.gene69309:CDS
TCAAATCCGTTTGTAAGTCT+TGG 0.415643 8.2:+16541719 MS.gene69309:CDS
TGCAGAGCATAAAGGAGAAA+AGG 0.417056 8.2:-16542526 None:intergenic
ATTAGCAAAGTGGGAATCAT+TGG 0.418936 8.2:-16541755 None:intergenic
TAATGGGGCTATTCATTGGT+TGG 0.427301 8.2:+16542257 MS.gene69309:CDS
TCTGAACATGAGCCCAAAGC+TGG 0.430995 8.2:+16542225 MS.gene69309:CDS
AACCTTACGATTCTGGTTTG+TGG 0.435471 8.2:+16542376 MS.gene69309:CDS
TCATCTTCATACATGTCTCT+TGG 0.436970 8.2:+16542134 MS.gene69309:CDS
CGCTCCTATAACGAATCATA+TGG 0.447526 8.2:+16542627 MS.gene69309:CDS
ACTTCCCGGTGACAATCAAC+AGG 0.452216 8.2:+16542686 MS.gene69309:CDS
TTAAGCCTGTTGATTGTCAC+CGG 0.471883 8.2:-16542691 None:intergenic
GCAGAGCATAAAGGAGAAAA+GGG 0.474610 8.2:-16542525 None:intergenic
ATTCCTTTCAATTAAATCAA+AGG 0.474723 8.2:-16542316 None:intergenic
AGATCTGAATAAGAACCTAT+AGG 0.488438 8.2:-16541913 None:intergenic
TGTTTAAGCTTATATGGCAC+AGG 0.490002 8.2:-16542351 None:intergenic
TATACAAAGTGCATAAGTCT+TGG 0.491874 8.2:+16542463 MS.gene69309:CDS
GAAAATGTGAATTAGCAAAG+TGG 0.503455 8.2:-16541765 None:intergenic
TGATCATCCAAATCCTACTA+AGG 0.508287 8.2:+16541671 MS.gene69309:CDS
GGGTGTTGAATGCCATCAAA+AGG 0.508697 8.2:-16542504 None:intergenic
AAAATGTGAATTAGCAAAGT+GGG 0.509421 8.2:-16541764 None:intergenic
TTGATATGAGCAAAACTCTA+CGG 0.515326 8.2:-16541810 None:intergenic
TTGCCTTTGATTTAATTGAA+AGG 0.522505 8.2:+16542313 MS.gene69309:CDS
GTCAACAGATGATTATTTGG+TGG 0.525175 8.2:+16542098 MS.gene69309:CDS
AACTCTACGGGTGTGTGTTG+CGG 0.533131 8.2:-16541797 None:intergenic
TCTCATTGAGAACTAATACA+TGG 0.535970 8.2:+16542163 MS.gene69309:CDS
AACAGATGATCCTAACAGAT+TGG 0.540285 8.2:+16542569 MS.gene69309:CDS
AGCTGGGTTGTTCTTTAATG+GGG 0.543964 8.2:+16542242 MS.gene69309:CDS
TGATATGAGCAAAACTCTAC+GGG 0.546554 8.2:-16541809 None:intergenic
TCGTGGTGCAGCTGAAATAT+GGG 0.550093 8.2:+16542431 MS.gene69309:CDS
AATACATGGAAACAAATTCA+GGG 0.551639 8.2:+16542177 MS.gene69309:CDS
AGATTGGTGAAGTATAATCA+TGG 0.563280 8.2:+16542585 MS.gene69309:CDS
ATCAATTCATGAGGCAGATG+CGG 0.573527 8.2:-16541652 None:intergenic
AGAAAACCAAGACTTACAAA+CGG 0.574732 8.2:-16541725 None:intergenic
CGAATCATATGGACCTGAAG+TGG 0.577896 8.2:+16542638 MS.gene69309:CDS
CTGAACATGAGCCCAAAGCT+GGG 0.581867 8.2:+16542226 MS.gene69309:CDS
CCAAATCCTACTAAGGTCAC+CGG 0.589229 8.2:+16541678 MS.gene69309:CDS
ACCCACAAACCAGAATCGTA+AGG 0.614833 8.2:-16542378 None:intergenic
AAACGTACAAGAGACTTCAC+CGG 0.618682 8.2:-16541697 None:intergenic
TCTACGGGTGTGTGTTGCGG+CGG 0.636793 8.2:-16541794 None:intergenic
GAGACATGTATGAAGATGAA+CGG 0.643604 8.2:-16542130 None:intergenic
TAAGCCTGTTGATTGTCACC+GGG 0.651245 8.2:-16542690 None:intergenic
AAGCAGAGACTCTGTATACA+CGG 0.652046 8.2:-16542661 None:intergenic
ATTTGGATGATCAATTCATG+AGG 0.666883 8.2:-16541661 None:intergenic
CTTGGTTTGTGGGCTCATCG+TGG 0.695842 8.2:+16542414 MS.gene69309:CDS
GCAGATGCATACATGCAATG+AGG 0.696847 8.2:-16542201 None:intergenic
CAGAGCATAAAGGAGAAAAG+GGG 0.698195 8.2:-16542524 None:intergenic
TTGGTGAAGTATAATCATGG+AGG 0.729522 8.2:+16542588 MS.gene69309:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCCTTTCAATTAAATCAA+AGG - chr8.2:16542319-16542338 None:intergenic 20.0%
!! TATTCAGATCTTGAAATTAA+AGG + chr8.2:16541925-16541944 MS.gene69309:CDS 20.0%
!!! GCAAAAAAAGGTATTATTAT+TGG + chr8.2:16542546-16542565 MS.gene69309:CDS 20.0%
!!! GTTCTTTAAGCTTTTATATA+TGG + chr8.2:16541977-16541996 MS.gene69309:intron 20.0%
!!! TATTTTTTTATGAAATGCAG+TGG - chr8.2:16542010-16542029 None:intergenic 20.0%
! AAAATGTGAATTAGCAAAGT+GGG - chr8.2:16541767-16541786 None:intergenic 25.0%
! AATACATGGAAACAAATTCA+GGG + chr8.2:16542177-16542196 MS.gene69309:CDS 25.0%
! TAATACATGGAAACAAATTC+AGG + chr8.2:16542176-16542195 MS.gene69309:CDS 25.0%
!! AAGGAATGTGAATATTTCTA+TGG + chr8.2:16542060-16542079 MS.gene69309:CDS 25.0%
!! TTGCCTTTGATTTAATTGAA+AGG + chr8.2:16542313-16542332 MS.gene69309:CDS 25.0%
!!! GATTTTGATTTTCTACCTAT+AGG + chr8.2:16541898-16541917 MS.gene69309:CDS 25.0%
!!! GTGAATATTTCTATGGTTTT+GGG + chr8.2:16542067-16542086 MS.gene69309:CDS 25.0%
!!! TGTGAATATTTCTATGGTTT+TGG + chr8.2:16542066-16542085 MS.gene69309:CDS 25.0%
AAATTAAAGGTTCGTGTAGA+GGG + chr8.2:16541938-16541957 MS.gene69309:intron 30.0%
AGAAAACCAAGACTTACAAA+CGG - chr8.2:16541728-16541747 None:intergenic 30.0%
AGATCTGAATAAGAACCTAT+AGG - chr8.2:16541916-16541935 None:intergenic 30.0%
AGATTGGTGAAGTATAATCA+TGG + chr8.2:16542585-16542604 MS.gene69309:CDS 30.0%
ATTTGGATGATCAATTCATG+AGG - chr8.2:16541664-16541683 None:intergenic 30.0%
GAAAATGTGAATTAGCAAAG+TGG - chr8.2:16541768-16541787 None:intergenic 30.0%
GGTTCATGTTTAAGCTTATA+TGG - chr8.2:16542360-16542379 None:intergenic 30.0%
TATACAAAGTGCATAAGTCT+TGG + chr8.2:16542463-16542482 MS.gene69309:CDS 30.0%
TCTCATTGAGAACTAATACA+TGG + chr8.2:16542163-16542182 MS.gene69309:CDS 30.0%
TTATACTTCACCAATCTGTT+AGG - chr8.2:16542582-16542601 None:intergenic 30.0%
TTGATATGAGCAAAACTCTA+CGG - chr8.2:16541813-16541832 None:intergenic 30.0%
! ACTCATGTTTTTCCTTTTGA+TGG + chr8.2:16542492-16542511 MS.gene69309:CDS 30.0%
! TTTTATGAAATGCAGTGGAT+GGG - chr8.2:16542005-16542024 None:intergenic 30.0%
! TTTTTATGAAATGCAGTGGA+TGG - chr8.2:16542006-16542025 None:intergenic 30.0%
!!! TTTTCGTCTTGATTTGCTAA+AGG + chr8.2:16542281-16542300 MS.gene69309:CDS 30.0%
AAACATGAACCTTACGATTC+TGG + chr8.2:16542369-16542388 MS.gene69309:CDS 35.0%
AAAGCTGGGTTGTTCTTTAA+TGG + chr8.2:16542240-16542259 MS.gene69309:CDS 35.0%
AACAGATGATCCTAACAGAT+TGG + chr8.2:16542569-16542588 MS.gene69309:CDS 35.0%
AAGCTGGGTTGTTCTTTAAT+GGG + chr8.2:16542241-16542260 MS.gene69309:CDS 35.0%
AGGTCCATATGATTCGTTAT+AGG - chr8.2:16542634-16542653 None:intergenic 35.0%
ATTAAAGAACAACCCAGCTT+TGG - chr8.2:16542241-16542260 None:intergenic 35.0%
ATTAGCAAAGTGGGAATCAT+TGG - chr8.2:16541758-16541777 None:intergenic 35.0%
CCTTTATGCTCTGCAAAAAA+AGG + chr8.2:16542534-16542553 MS.gene69309:CDS 35.0%
CTCGTCAACAGATGATTATT+TGG + chr8.2:16542095-16542114 MS.gene69309:CDS 35.0%
GAAATTAAAGGTTCGTGTAG+AGG + chr8.2:16541937-16541956 MS.gene69309:intron 35.0%
GAGACATGTATGAAGATGAA+CGG - chr8.2:16542133-16542152 None:intergenic 35.0%
GTCAACAGATGATTATTTGG+TGG + chr8.2:16542098-16542117 MS.gene69309:CDS 35.0%
TCAAATCCGTTTGTAAGTCT+TGG + chr8.2:16541719-16541738 MS.gene69309:CDS 35.0%
TCATCTTCATACATGTCTCT+TGG + chr8.2:16542134-16542153 MS.gene69309:CDS 35.0%
TCTTTAATGGGGCTATTCAT+TGG + chr8.2:16542253-16542272 MS.gene69309:CDS 35.0%
TGATATGAGCAAAACTCTAC+GGG - chr8.2:16541812-16541831 None:intergenic 35.0%
TGATCATCCAAATCCTACTA+AGG + chr8.2:16541671-16541690 MS.gene69309:CDS 35.0%
TGGGTATTTGGAGAATTTCT+TGG + chr8.2:16542396-16542415 MS.gene69309:CDS 35.0%
TGTTGAGTGTGATTCAGATA+AGG + chr8.2:16542041-16542060 MS.gene69309:intron 35.0%
TGTTTAAGCTTATATGGCAC+AGG - chr8.2:16542354-16542373 None:intergenic 35.0%
TTAAAGAACAACCCAGCTTT+GGG - chr8.2:16542240-16542259 None:intergenic 35.0%
TTATCTGAATCACACTCAAC+AGG - chr8.2:16542042-16542061 None:intergenic 35.0%
TTGGTGAAGTATAATCATGG+AGG + chr8.2:16542588-16542607 MS.gene69309:CDS 35.0%
! TTGGAGAATTTCTTGGTTTG+TGG + chr8.2:16542403-16542422 MS.gene69309:CDS 35.0%
!! TGGAGAATTTCTTGGTTTGT+GGG + chr8.2:16542404-16542423 MS.gene69309:CDS 35.0%
!!! CCTTTTTTTGCAGAGCATAA+AGG - chr8.2:16542537-16542556 None:intergenic 35.0%
AAACGTACAAGAGACTTCAC+CGG - chr8.2:16541700-16541719 None:intergenic 40.0%
AAGCAGAGACTCTGTATACA+CGG - chr8.2:16542664-16542683 None:intergenic 40.0%
AGCTGGGTTGTTCTTTAATG+GGG + chr8.2:16542242-16542261 MS.gene69309:CDS 40.0%
ATCAATTCATGAGGCAGATG+CGG - chr8.2:16541655-16541674 None:intergenic 40.0%
CAGAGCATAAAGGAGAAAAG+GGG - chr8.2:16542527-16542546 None:intergenic 40.0%
CGCTCCTATAACGAATCATA+TGG + chr8.2:16542627-16542646 MS.gene69309:CDS 40.0%
GCAGAGCATAAAGGAGAAAA+GGG - chr8.2:16542528-16542547 None:intergenic 40.0%
TAATGGGGCTATTCATTGGT+TGG + chr8.2:16542257-16542276 MS.gene69309:CDS 40.0%
TGCAGAGCATAAAGGAGAAA+AGG - chr8.2:16542529-16542548 None:intergenic 40.0%
! TTAAGCCTGTTGATTGTCAC+CGG - chr8.2:16542694-16542713 None:intergenic 40.0%
!! AACCTTACGATTCTGGTTTG+TGG + chr8.2:16542376-16542395 MS.gene69309:CDS 40.0%
!! ACCTTACGATTCTGGTTTGT+GGG + chr8.2:16542377-16542396 MS.gene69309:CDS 40.0%
!! GATTCTGGTTTGTGGGTATT+TGG + chr8.2:16542384-16542403 MS.gene69309:CDS 40.0%
ACCCACAAACCAGAATCGTA+AGG - chr8.2:16542381-16542400 None:intergenic 45.0%
ATCGTGGTGCAGCTGAAATA+TGG + chr8.2:16542430-16542449 MS.gene69309:CDS 45.0%
CCAAATCCTACTAAGGTCAC+CGG + chr8.2:16541678-16541697 MS.gene69309:CDS 45.0%
CGAATCATATGGACCTGAAG+TGG + chr8.2:16542638-16542657 MS.gene69309:CDS 45.0%
GCAGATGCATACATGCAATG+AGG - chr8.2:16542204-16542223 None:intergenic 45.0%
TCACACTCAACAGGAGACAA+AGG - chr8.2:16542033-16542052 None:intergenic 45.0%
TCGTGGTGCAGCTGAAATAT+GGG + chr8.2:16542431-16542450 MS.gene69309:CDS 45.0%
! TAAGCCTGTTGATTGTCACC+GGG - chr8.2:16542693-16542712 None:intergenic 45.0%
!! GGGTGTTGAATGCCATCAAA+AGG - chr8.2:16542507-16542526 None:intergenic 45.0%
AACTCTACGGGTGTGTGTTG+CGG - chr8.2:16541800-16541819 None:intergenic 50.0%
ACTTCACCGGTGACCTTAGT+AGG - chr8.2:16541687-16541706 None:intergenic 50.0%
ACTTCCCGGTGACAATCAAC+AGG + chr8.2:16542686-16542705 MS.gene69309:CDS 50.0%
CCGGTGACCTTAGTAGGATT+TGG - chr8.2:16541681-16541700 None:intergenic 50.0%
CTGAACATGAGCCCAAAGCT+GGG + chr8.2:16542226-16542245 MS.gene69309:CDS 50.0%
GAGTCTCTGCTTTCACTTCC+CGG + chr8.2:16542672-16542691 MS.gene69309:CDS 50.0%
TCTGAACATGAGCCCAAAGC+TGG + chr8.2:16542225-16542244 MS.gene69309:CDS 50.0%
TCTGTATACACGGCCACTTC+AGG - chr8.2:16542654-16542673 None:intergenic 50.0%
!! CTTGGTTTGTGGGCTCATCG+TGG + chr8.2:16542414-16542433 MS.gene69309:CDS 55.0%
TCTACGGGTGTGTGTTGCGG+CGG - chr8.2:16541797-16541816 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 16541634 16542713 16541634 ID=MS.gene69309
chr8.2 mRNA 16541634 16542713 16541634 ID=MS.gene69309.t1;Parent=MS.gene69309
chr8.2 exon 16541634 16541946 16541634 ID=MS.gene69309.t1.exon1;Parent=MS.gene69309.t1
chr8.2 CDS 16541634 16541946 16541634 ID=cds.MS.gene69309.t1;Parent=MS.gene69309.t1
chr8.2 exon 16542058 16542713 16542058 ID=MS.gene69309.t1.exon2;Parent=MS.gene69309.t1
chr8.2 CDS 16542058 16542713 16542058 ID=cds.MS.gene69309.t1;Parent=MS.gene69309.t1
Gene Sequence

>MS.gene69309

ATGGAGACGACGACGCTGCCGCATCTGCCTCATGAATTGATCATCCAAATCCTACTAAGGTCACCGGTGAAGTCTCTTGTACGTTTCAAATCCGTTTGTAAGTCTTGGTTTTCTCTTATCTCCAATGATTCCCACTTTGCTAATTCACATTTTCAACTTTCCGCCGCAACACACACCCGTAGAGTTTTGCTCATATCAACTTCAGCTTTTGAATCTATAGATTTAGAAGCATCGCTTCATGACGATTCTGCTTTTGCTTCACTAGATTTTGATTTTCTACCTATAGGTTCTTATTCAGATCTTGAAATTAAAGGTTCGTGTAGAGGGTTTATACTTTTGCACTGTTCTTTAAGCTTTTATATATGGAACCCATCCACTGCATTTCATAAAAAAATACCTTTGTCTCCTGTTGAGTGTGATTCAGATAAGGAATGTGAATATTTCTATGGTTTTGGGTATGACTCGTCAACAGATGATTATTTGGTGGTTTCAATGTCCGTTCATCTTCATACATGTCTCTTGGAGATTTTCTCATTGAGAACTAATACATGGAAACAAATTCAGGGTCCTCATTGCATGTATGCATCTGCTTCTGAACATGAGCCCAAAGCTGGGTTGTTCTTTAATGGGGCTATTCATTGGTTGGCTTTTCGTCTTGATTTGCTAAAGGATGTTATTGTTGCCTTTGATTTAATTGAAAGGAATTTTTTTTGTATGCCTGTGCCATATAAGCTTAAACATGAACCTTACGATTCTGGTTTGTGGGTATTTGGAGAATTTCTTGGTTTGTGGGCTCATCGTGGTGCAGCTGAAATATGGGTGATGAAAGTATACAAAGTGCATAAGTCTTGGACAAACACTCATGTTTTTCCTTTTGATGGCATTCAACACCCCTTTTCTCCTTTATGCTCTGCAAAAAAAGGTATTATTATTGGAACAGATGATCCTAACAGATTGGTGAAGTATAATCATGGAGGAAAGTTGTTAGAGTGTCGCTCCTATAACGAATCATATGGACCTGAAGTGGCCGTGTATACAGAGTCTCTGCTTTCACTTCCCGGTGACAATCAACAGGCTTAA
Protein sequence

>MS.gene69309.t1

METTTLPHLPHELIIQILLRSPVKSLVRFKSVCKSWFSLISNDSHFANSHFQLSAATHTRRVLLISTSAFESIDLEASLHDDSAFASLDFDFLPIGSYSDLEIKDKECEYFYGFGYDSSTDDYLVVSMSVHLHTCLLEIFSLRTNTWKQIQGPHCMYASASEHEPKAGLFFNGAIHWLAFRLDLLKDVIVAFDLIERNFFCMPVPYKLKHEPYDSGLWVFGEFLGLWAHRGAAEIWVMKVYKVHKSWTNTHVFPFDGIQHPFSPLCSAKKGIIIGTDDPNRLVKYNHGGKLLECRSYNESYGPEVAVYTESLLSLPGDNQQA