AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070461

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070461.t1 MTR_3g034160 88.782 312 34 1 1 312 64 374 0.0 553
MS.gene070461.t1 MTR_3g034180 85.897 312 43 1 1 312 178 488 0.0 553
MS.gene070461.t1 MTR_3g034130 85.284 299 44 0 1 299 64 362 0.0 522
MS.gene070461.t1 MTR_3g103020 62.540 315 103 6 3 313 75 378 3.61e-130 375
MS.gene070461.t1 MTR_4g117720 61.728 324 98 11 1 313 70 378 9.01e-124 359
MS.gene070461.t1 MTR_6g060650 60.559 322 106 6 1 312 62 372 1.68e-119 348
MS.gene070461.t1 MTR_4g109270 56.113 319 116 7 1 313 64 364 1.09e-108 320
MS.gene070461.t1 MTR_4g109280 59.807 311 106 8 6 313 65 359 8.40e-108 318
MS.gene070461.t1 MTR_4g122850 54.777 314 125 9 6 313 62 364 5.59e-104 309
MS.gene070461.t1 MTR_4g117770 55.949 311 117 5 9 313 71 367 4.42e-103 306
MS.gene070461.t1 MTR_4g117810 55.949 311 117 5 9 313 71 367 4.42e-103 306
MS.gene070461.t1 MTR_4g117850 54.575 306 116 8 7 306 85 373 2.80e-99 297
MS.gene070461.t1 MTR_4g094365 51.411 319 132 7 1 313 63 364 1.56e-98 295
MS.gene070461.t1 MTR_4g117780 53.205 312 124 6 9 313 71 367 4.78e-98 293
MS.gene070461.t1 MTR_4g118380 53.642 302 108 6 6 286 67 357 1.22e-96 290
MS.gene070461.t1 MTR_4g023090 49.544 329 131 8 1 311 71 382 2.13e-95 287
MS.gene070461.t1 MTR_4g023080 52.548 314 123 8 1 312 64 353 2.51e-95 286
MS.gene070461.t1 MTR_4g021785 50.794 315 130 7 1 313 64 355 2.07e-93 281
MS.gene070461.t1 MTR_5g097210 49.508 305 138 6 7 310 71 360 2.33e-92 279
MS.gene070461.t1 MTR_5g070080 49.699 332 129 8 6 313 69 386 5.96e-91 276
MS.gene070461.t1 MTR_4g023380 47.801 341 118 9 1 313 71 379 1.55e-90 275
MS.gene070461.t1 MTR_4g116330 50.321 312 134 7 8 313 84 380 1.15e-89 273
MS.gene070461.t1 MTR_4g117870 49.363 314 111 7 1 313 1 267 1.55e-86 261
MS.gene070461.t1 MTR_5g070130 48.910 321 138 11 6 313 70 377 1.95e-86 265
MS.gene070461.t1 MTR_4g023070 47.720 329 138 10 1 312 58 369 3.36e-85 261
MS.gene070461.t1 MTR_1g055135 46.984 315 147 8 7 313 73 375 2.14e-83 256
MS.gene070461.t1 MTR_4g117750 48.718 312 131 7 7 309 64 355 3.57e-83 259
MS.gene070461.t1 MTR_4g117750 60.550 109 41 1 205 311 373 481 1.98e-34 131
MS.gene070461.t1 MTR_4g023130 53.875 271 101 7 44 312 64 312 9.61e-83 253
MS.gene070461.t1 MTR_4g023120 47.256 328 143 9 1 313 58 370 1.07e-82 254
MS.gene070461.t1 MTR_1g055195 45.625 320 143 7 2 313 182 478 1.11e-79 250
MS.gene070461.t1 MTR_8g433040 48.397 312 128 9 9 313 79 364 1.14e-79 246
MS.gene070461.t1 MTR_8g032380 48.397 312 128 9 9 313 79 364 1.14e-79 246
MS.gene070461.t1 MTR_4g023190 48.580 317 133 11 1 311 65 357 8.41e-79 244
MS.gene070461.t1 MTR_5g070088 45.181 332 147 9 6 313 66 386 1.98e-78 244
MS.gene070461.t1 MTR_4g023440 46.006 313 115 7 1 313 74 332 2.19e-77 239
MS.gene070461.t1 MTR_4g023200 47.319 317 138 11 1 311 65 358 2.58e-77 240
MS.gene070461.t1 MTR_4g023150 47.003 317 139 11 1 311 65 358 1.79e-75 235
MS.gene070461.t1 MTR_4g118400 47.000 300 113 7 16 307 33 294 8.09e-75 232
MS.gene070461.t1 MTR_8g032390 46.349 315 113 11 7 308 99 370 1.85e-74 233
MS.gene070461.t1 MTR_4g116320 56.250 224 83 6 91 310 43 255 2.02e-73 227
MS.gene070461.t1 MTR_8g040935 55.556 207 80 4 113 313 8 208 1.28e-69 215
MS.gene070461.t1 MTR_7g079400 42.395 309 155 8 14 313 43 337 1.83e-69 219
MS.gene070461.t1 MTR_8g013010 56.944 216 76 6 1 214 109 309 1.87e-67 213
MS.gene070461.t1 MTR_4g118370 44.643 280 123 6 33 310 8 257 2.63e-67 211
MS.gene070461.t1 MTR_8g433030 53.097 226 87 8 88 308 126 337 5.15e-63 203
MS.gene070461.t1 MTR_7g079370 41.195 318 155 9 7 307 48 350 8.49e-62 200
MS.gene070461.t1 MTR_4g121580 45.098 306 118 13 7 303 75 339 3.27e-61 200
MS.gene070461.t1 MTR_4g116330 42.628 312 119 8 8 313 84 341 1.16e-60 197
MS.gene070461.t1 MTR_6g463430 51.759 199 82 4 118 313 15 202 3.75e-54 176
MS.gene070461.t1 MTR_8g102520 39.048 315 144 10 7 313 48 322 8.31e-53 176
MS.gene070461.t1 MTR_1g055175 47.500 200 95 5 118 313 8 201 1.14e-52 172
MS.gene070461.t1 MTR_1g055250 41.558 231 120 8 74 300 34 253 1.49e-49 167
MS.gene070461.t1 MTR_4g117530 38.621 290 118 9 28 313 20 253 2.07e-49 165
MS.gene070461.t1 MTR_4g117430 50.575 174 63 4 46 217 3 155 1.08e-48 160
MS.gene070461.t1 MTR_1g055165 50.920 163 77 3 153 313 157 318 1.06e-45 157
MS.gene070461.t1 MTR_4g114700 39.405 269 102 6 42 304 5 218 3.00e-45 154
MS.gene070461.t1 MTR_4g116340 48.603 179 76 5 7 180 80 247 5.94e-42 145
MS.gene070461.t1 MTR_2g048580 34.615 312 154 13 10 313 73 342 6.18e-42 148
MS.gene070461.t1 MTR_4g118360 57.031 128 49 3 190 313 1 126 1.69e-40 138
MS.gene070461.t1 MTR_7g079420 32.982 285 130 8 45 313 905 1144 1.00e-36 140
MS.gene070461.t1 MTR_4g122860 41.206 199 75 8 123 313 156 320 1.35e-35 131
MS.gene070461.t1 MTR_8g098830 41.954 174 87 5 114 282 30 194 6.86e-35 127
MS.gene070461.t1 MTR_5g023000 65.591 93 32 0 221 313 7 99 2.14e-33 119
MS.gene070461.t1 MTR_6g034720 45.000 160 69 5 40 196 31 174 3.32e-30 114
MS.gene070461.t1 MTR_7g117720 51.049 143 56 6 7 145 52 184 4.18e-30 115
MS.gene070461.t1 MTR_7g117720 40.268 149 75 6 164 307 132 271 1.49e-17 81.3
MS.gene070461.t1 MTR_7g079450 41.060 151 64 5 174 313 1 137 5.37e-25 98.2
MS.gene070461.t1 MTR_1g068875 29.278 263 141 13 3 243 52 291 2.17e-21 93.6
MS.gene070461.t1 MTR_5g069120 43.411 129 64 2 6 127 51 177 2.42e-20 87.0
MS.gene070461.t1 MTR_4g122840 41.176 136 42 4 181 313 164 264 2.53e-20 89.0
MS.gene070461.t1 MTR_2g028130 27.273 253 146 10 37 264 100 339 2.72e-19 87.8
MS.gene070461.t1 MTR_4g133170 28.958 259 146 14 2 242 51 289 4.24e-19 87.4
MS.gene070461.t1 MTR_4g023430 53.659 82 35 1 235 313 141 222 7.10e-19 84.0
MS.gene070461.t1 MTR_0667s0010 25.597 293 175 11 4 268 57 334 7.29e-19 86.7
MS.gene070461.t1 MTR_2g028160 26.882 279 166 10 21 274 86 351 8.22e-19 86.7
MS.gene070461.t1 MTR_2g426020 25.524 286 172 11 18 276 70 341 9.46e-19 86.3
MS.gene070461.t1 MTR_2g024110 25.660 265 163 11 23 269 76 324 2.26e-18 85.1
MS.gene070461.t1 MTR_8g013005 67.797 59 19 0 255 313 1 59 4.89e-18 77.0
MS.gene070461.t1 MTR_2g025960 25.830 271 158 10 39 282 1 255 8.99e-18 82.4
MS.gene070461.t1 MTR_5g037680 25.962 312 191 11 12 305 92 381 1.33e-17 83.2
MS.gene070461.t1 MTR_2g026010 27.203 261 162 11 32 276 78 326 1.80e-17 82.4
MS.gene070461.t1 MTR_2g426070 29.596 223 128 9 57 264 129 337 3.84e-17 81.6
MS.gene070461.t1 MTR_2g078870 31.500 200 107 9 44 238 58 232 1.51e-16 79.0
MS.gene070461.t1 MTR_2g031640 25.333 225 143 6 33 242 81 295 1.87e-16 79.7
MS.gene070461.t1 MTR_2g025420 23.776 286 169 10 23 274 86 356 1.88e-16 79.7
MS.gene070461.t1 MTR_3g083640 26.838 272 163 11 44 305 157 402 1.96e-16 79.7
MS.gene070461.t1 MTR_4g070550 25.965 285 163 11 3 250 53 326 2.30e-16 79.3
MS.gene070461.t1 MTR_1g055200 48.039 102 44 4 42 139 5 101 3.20e-16 73.6
MS.gene070461.t1 MTR_2g426090 22.684 313 188 11 2 276 43 339 4.28e-16 78.6
MS.gene070461.t1 MTR_2g021770 27.469 324 192 14 17 308 93 405 9.90e-16 77.4
MS.gene070461.t1 MTR_4g030830 24.752 303 165 13 44 305 113 393 1.10e-15 77.4
MS.gene070461.t1 MTR_1g068835 25.926 243 147 11 19 243 65 292 1.10e-15 77.4
MS.gene070461.t1 MTR_1g068860 24.895 237 151 9 21 243 71 294 1.11e-15 77.0
MS.gene070461.t1 MTR_4g117550 27.302 315 175 14 17 311 94 374 2.00e-15 76.3
MS.gene070461.t1 MTR_2g461450 27.477 222 135 8 34 248 99 301 8.28e-15 74.3
MS.gene070461.t1 MTR_2g028140 25.882 255 146 10 25 250 91 331 1.39e-14 74.3
MS.gene070461.t1 MTR_1g054725 28.571 231 133 11 21 238 95 306 1.56e-14 73.9
MS.gene070461.t1 MTR_2g043410 29.386 228 124 8 40 251 133 339 2.11e-14 73.6
MS.gene070461.t1 MTR_4g031770 27.602 221 127 10 31 242 93 289 2.64e-14 73.2
MS.gene070461.t1 MTR_7g056390 27.305 282 133 11 41 279 130 382 2.77e-14 73.2
MS.gene070461.t1 MTR_2g031720 23.377 308 197 11 2 280 43 340 2.97e-14 73.2
MS.gene070461.t1 MTR_2g021155 28.571 224 145 7 22 242 110 321 4.09e-14 73.6
MS.gene070461.t1 MTR_2g021155 28.261 184 116 5 59 238 711 882 5.74e-14 72.8
MS.gene070461.t1 MTR_3g010970 27.372 274 156 11 41 305 152 391 5.07e-14 72.4
MS.gene070461.t1 MTR_0044s0300 28.780 205 109 8 44 238 138 315 6.40e-14 72.0
MS.gene070461.t1 MTR_7g028660 27.509 269 169 9 21 273 88 346 1.11e-13 71.2
MS.gene070461.t1 MTR_3g083010 24.345 267 164 8 37 276 102 357 1.17e-13 71.2
MS.gene070461.t1 MTR_3g010870 28.037 214 123 9 32 238 122 311 1.26e-13 71.2
MS.gene070461.t1 MTR_4g055060 27.823 248 147 12 9 243 94 322 1.29e-13 71.2
MS.gene070461.t1 MTR_2g425980 27.948 229 132 10 54 262 119 334 1.40e-13 71.2
MS.gene070461.t1 MTR_3g030250 26.071 280 157 10 46 305 130 379 1.44e-13 70.9
MS.gene070461.t1 MTR_7g077910 25.652 230 137 7 41 251 42 256 2.14e-13 70.1
MS.gene070461.t1 MTR_7g070830 25.523 239 139 9 56 264 146 375 2.23e-13 70.5
MS.gene070461.t1 MTR_2g078900 30.601 183 98 9 59 238 153 309 2.31e-13 70.1
MS.gene070461.t1 MTR_7g070880 25.523 239 139 9 56 264 146 375 2.35e-13 70.5
MS.gene070461.t1 MTR_2g031680 25.333 225 142 7 32 242 80 292 4.02e-13 69.7
MS.gene070461.t1 MTR_1g094210 26.848 257 147 11 40 284 151 378 4.12e-13 69.7
MS.gene070461.t1 MTR_6g053240 25.121 207 131 6 41 238 125 316 4.85e-13 69.7
MS.gene070461.t1 MTR_4g023100 63.158 57 19 1 257 313 65 119 5.49e-13 65.1
MS.gene070461.t1 MTR_7g057270 26.800 250 139 10 31 248 107 344 5.58e-13 69.3
MS.gene070461.t1 MTR_2g021150 26.255 259 165 10 15 264 87 328 5.60e-13 69.3
MS.gene070461.t1 MTR_1g055145 47.297 74 37 2 157 229 130 202 1.21e-12 67.4
MS.gene070461.t1 MTR_7g445890 25.957 235 141 9 41 251 87 312 1.21e-12 68.2
MS.gene070461.t1 MTR_3g023930 27.206 272 154 11 44 305 130 367 1.34e-12 68.2
MS.gene070461.t1 MTR_3g036430 25.248 202 116 9 44 238 96 269 2.34e-12 67.0
MS.gene070461.t1 MTR_2g426060 25.000 264 151 12 33 264 88 336 2.63e-12 67.4
MS.gene070461.t1 MTR_8g099460 23.432 303 179 11 4 283 56 328 2.64e-12 67.4
MS.gene070461.t1 MTR_8g467670 25.163 306 189 12 17 303 88 372 2.65e-12 67.4
MS.gene070461.t1 MTR_5g022040 25.984 254 142 11 18 247 161 392 3.81e-12 67.0
MS.gene070461.t1 MTR_6g021880 27.397 219 141 6 44 255 122 329 3.81e-12 66.6
MS.gene070461.t1 MTR_6g027910 26.168 214 132 6 38 238 106 306 4.11e-12 66.6
MS.gene070461.t1 MTR_8g464770 25.000 308 196 11 17 307 88 377 6.40e-12 65.9
MS.gene070461.t1 MTR_3g030320 26.800 250 144 13 1 238 1 223 7.67e-12 65.5
MS.gene070461.t1 MTR_6g007135 25.403 248 155 10 17 252 100 329 9.91e-12 65.5
MS.gene070461.t1 MTR_4g024890 23.954 263 159 11 7 247 71 314 1.07e-11 65.5
MS.gene070461.t1 MTR_4g109290 60.377 53 21 0 261 313 136 188 1.44e-11 62.8
MS.gene070461.t1 MTR_6g053260 26.794 209 132 7 34 238 429 620 1.53e-11 65.5
MS.gene070461.t1 MTR_1g112010 25.000 248 142 9 15 250 48 263 1.55e-11 64.7
MS.gene070461.t1 MTR_4g028510 27.338 278 162 11 59 310 139 402 1.66e-11 65.1
MS.gene070461.t1 MTR_7g060960 26.667 225 112 11 41 243 106 299 1.81e-11 64.7
MS.gene070461.t1 MTR_3g030310 25.676 222 130 7 43 254 127 323 2.10e-11 64.7
MS.gene070461.t1 MTR_3g010590 27.230 213 119 10 32 238 124 306 2.26e-11 64.3
MS.gene070461.t1 MTR_3g006670 27.273 220 136 9 31 242 133 336 2.40e-11 64.3
MS.gene070461.t1 MTR_3g006500 26.190 294 167 11 32 305 106 369 2.99e-11 63.9
MS.gene070461.t1 MTR_3g006570 24.653 288 162 12 44 305 118 376 3.27e-11 63.9
MS.gene070461.t1 MTR_7g017040 24.224 322 198 14 16 306 88 394 3.88e-11 63.9
MS.gene070461.t1 MTR_5g097680 24.735 283 164 11 59 308 159 425 4.98e-11 63.5
MS.gene070461.t1 MTR_3g464470 23.967 242 131 8 57 267 121 340 5.17e-11 63.2
MS.gene070461.t1 MTR_0054s0090 26.132 287 167 11 56 311 135 407 5.76e-11 63.2
MS.gene070461.t1 MTR_2g043400 27.826 230 127 10 40 251 132 340 5.86e-11 63.2
MS.gene070461.t1 MTR_5g086590 26.263 198 123 7 54 242 11 194 7.12e-11 61.6
MS.gene070461.t1 MTR_3g096280 26.165 279 155 18 37 305 105 342 8.04e-11 62.4
MS.gene070461.t1 MTR_6g004660 26.582 237 149 9 18 243 93 315 8.49e-11 62.8
MS.gene070461.t1 MTR_2g064960 64.286 42 15 0 272 313 18 59 8.80e-11 57.0
MS.gene070461.t1 MTR_6g090180 24.735 283 167 9 54 306 168 434 9.06e-11 62.8
MS.gene070461.t1 MTR_4g013185 24.896 241 136 8 41 251 121 346 9.84e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070461.t1 AT3G06240 28.205 234 132 10 39 260 163 372 1.51e-16 80.1
MS.gene070461.t1 AT4G12560 27.451 255 163 10 40 276 90 340 6.22e-14 72.4
MS.gene070461.t1 AT4G12560 27.451 255 163 10 40 276 90 340 6.22e-14 72.4
MS.gene070461.t1 AT4G12560 27.451 255 163 10 40 276 90 340 6.22e-14 72.4

Find 69 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCGATGCTTCAAAATTTA+TGG 0.085730 3.3:+24972320 None:intergenic
TCATTTGCATTCATATAATA+AGG 0.164755 3.3:+24971919 None:intergenic
TTGGAGAATTTCTAAGTCTA+TGG 0.196739 3.3:-24971726 MS.gene070461:CDS
GATTGTGATTTGTGGATATT+TGG 0.226311 3.3:-24971745 MS.gene070461:CDS
TGAATATTGGCCATCTAAAT+TGG 0.231444 3.3:+24972125 None:intergenic
AGGATGGAACAATTGAAATA+TGG 0.245076 3.3:-24971696 MS.gene070461:CDS
TCATGTTGTCTGTATAGTTT+TGG 0.248650 3.3:-24972105 MS.gene070461:CDS
GTTCTTCAAGCATTTATCTA+TGG 0.293765 3.3:-24972191 MS.gene070461:CDS
AAAGTAGGGACGCTCTTTAA+TGG 0.309047 3.3:-24971889 MS.gene070461:CDS
AGATAATATTTCATCACATT+TGG 0.320818 3.3:-24972013 MS.gene070461:CDS
TTTCCTCCTTCCAATTTAGA+TGG 0.347200 3.3:-24972135 MS.gene070461:CDS
ATTAAAGAGCGTCCCTACTT+TGG 0.358272 3.3:+24971891 None:intergenic
TATTGTTGGCTTTGATTTAG+TGG 0.370118 3.3:-24971821 MS.gene070461:CDS
TATACAGACAACATGAATAT+TGG 0.371093 3.3:+24972112 None:intergenic
TTCAAGATTAGAATAAGATT+CGG 0.374154 3.3:+24972251 None:intergenic
TTAAAGAGCGTCCCTACTTT+GGG 0.374951 3.3:+24971892 None:intergenic
TCAAGATTAGAATAAGATTC+GGG 0.381250 3.3:+24972252 None:intergenic
TCTTTAATGGGACTATTCAT+TGG 0.384035 3.3:-24971876 MS.gene070461:CDS
TGGAAATGTCATCAATAGAT+AGG 0.410119 3.3:+24971626 None:intergenic
TGAGCCTTTACTCGTATCAT+AGG 0.419178 3.3:+24972041 None:intergenic
AACCTATAGATTGTGATTTG+TGG 0.425796 3.3:-24971753 MS.gene070461:CDS
AAGTAGGGACGCTCTTTAAT+GGG 0.430028 3.3:-24971888 MS.gene070461:CDS
AGGTATTTGTTTGTGATGTC+CGG 0.430912 3.3:+24972158 None:intergenic
GGATGGAACAATTGAAATAT+GGG 0.445935 3.3:-24971695 MS.gene070461:CDS
CATTTGCATTCATATAATAA+GGG 0.448554 3.3:+24971920 None:intergenic
GGTTCATGGTCAAACCCATC+TGG 0.449525 3.3:+24971772 None:intergenic
GAGTCTCTGCTTTCACTCCC+TGG 0.452167 3.3:-24971448 MS.gene070461:CDS
AATACAAAGTGCATTCGTCT+TGG 0.453817 3.3:-24971663 MS.gene070461:CDS
ATACAAAGTGCATTCGTCTT+GGG 0.454030 3.3:-24971662 MS.gene070461:CDS
AATCACAATCTATAGGTTCA+TGG 0.456461 3.3:+24971758 None:intergenic
ATCCACAAATCACAATCTAT+AGG 0.460799 3.3:+24971751 None:intergenic
TCTAAATTGGAAGGAGGAAA+AGG 0.467688 3.3:+24972138 None:intergenic
ATTGGAACAAACGGTGGCAC+TGG 0.477184 3.3:-24971559 MS.gene070461:CDS
TTATCAATGGATGTTATTGT+TGG 0.479970 3.3:-24971835 MS.gene070461:CDS
CTCTGTATACATAGCCACTA+GGG 0.483092 3.3:+24971468 None:intergenic
TGGAGAATTTCTAAGTCTAT+GGG 0.490268 3.3:-24971725 MS.gene070461:CDS
TGGTCAAACCCATCTGGAAA+AGG 0.491312 3.3:+24971778 None:intergenic
TGATTATACTTCACCAATCC+AGG 0.492432 3.3:+24971946 None:intergenic
CCATTCAAGAGATGATTACT+TGG 0.492530 3.3:-24972076 MS.gene070461:CDS
ATTTCTAAGTCTATGGGCTA+TGG 0.507502 3.3:-24971719 MS.gene070461:CDS
GCAAATGATGATCCCAAAGT+AGG 0.511115 3.3:-24971904 MS.gene070461:CDS
TGGAAAGATATTGAGTGTCC+TGG 0.515552 3.3:-24971964 MS.gene070461:CDS
CCAAGTAATCATCTCTTGAA+TGG 0.518868 3.3:+24972076 None:intergenic
TTTCATTGAGAGCTAATACA+TGG 0.531728 3.3:-24971984 MS.gene070461:CDS
AGTCTATGGGCTATGGAGGA+TGG 0.532601 3.3:-24971712 MS.gene070461:CDS
GAAATTAAAAGTTCATGTAG+AGG 0.538733 3.3:-24972231 MS.gene070461:CDS
ATTCGGGAGGCATAAAATCA+AGG 0.540291 3.3:+24972268 None:intergenic
GGTGGTATTATTGGAACAAA+CGG 0.557309 3.3:-24971568 MS.gene070461:CDS
TTCAAGAGATGATTACTTGG+TGG 0.560339 3.3:-24972073 MS.gene070461:CDS
TTGGCTTTGATTTAGTGGAA+AGG 0.565737 3.3:-24971816 MS.gene070461:CDS
CAAATGATGATCCCAAAGTA+GGG 0.565983 3.3:-24971903 MS.gene070461:CDS
TATTGGCCATCTAAATTGGA+AGG 0.567363 3.3:+24972129 None:intergenic
AACAAACGGTGGCACTGGAT+TGG 0.570072 3.3:-24971554 MS.gene070461:CDS
AGATATTGAGTGTCCTGGAT+TGG 0.574538 3.3:-24971959 MS.gene070461:CDS
TCTAAGTCTATGGGCTATGG+AGG 0.577648 3.3:-24971716 MS.gene070461:CDS
TGTTTGTGATGTCCGGTCGA+TGG 0.582419 3.3:+24972165 None:intergenic
TAAGCTTGATCACTGTCACC+AGG 0.583359 3.3:+24971430 None:intergenic
ACTGGATTGGTGAAGTATGA+TGG 0.588559 3.3:-24971541 MS.gene070461:CDS
TATCTATGGAATCCATCGAC+CGG 0.592196 3.3:-24972177 MS.gene070461:CDS
AAGCTTGATCACTGTCACCA+GGG 0.598347 3.3:+24971431 None:intergenic
GTGTCCTATGATACGAGTAA+AGG 0.602933 3.3:-24972045 MS.gene070461:CDS
ACTCTGTATACATAGCCACT+AGG 0.618200 3.3:+24971467 None:intergenic
TGGCCATCTAAATTGGAAGG+AGG 0.651013 3.3:+24972132 None:intergenic
CGAACCATGTGAACCCCTAG+TGG 0.657467 3.3:-24971482 MS.gene070461:CDS
AGATTAGAATAAGATTCGGG+AGG 0.659056 3.3:+24972255 None:intergenic
TCTGTATACATAGCCACTAG+GGG 0.693139 3.3:+24971469 None:intergenic
ATAGCCACTAGGGGTTCACA+TGG 0.697094 3.3:+24971478 None:intergenic
GGTATTATTGGAACAAACGG+TGG 0.700731 3.3:-24971565 MS.gene070461:CDS
TTGGTGAAGTATGATGGCAA+TGG 0.739876 3.3:-24971535 MS.gene070461:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATAATATTTCATCACATT+TGG - chr3.3:24971764-24971783 MS.gene070461:CDS 20.0%
!! CATTTGCATTCATATAATAA+GGG + chr3.3:24971860-24971879 None:intergenic 20.0%
!! TCATTTGCATTCATATAATA+AGG + chr3.3:24971861-24971880 None:intergenic 20.0%
!! TTCAAGATTAGAATAAGATT+CGG + chr3.3:24971529-24971548 None:intergenic 20.0%
! GAAATTAAAAGTTCATGTAG+AGG - chr3.3:24971546-24971565 MS.gene070461:CDS 25.0%
! TATACAGACAACATGAATAT+TGG + chr3.3:24971668-24971687 None:intergenic 25.0%
! TCAAGATTAGAATAAGATTC+GGG + chr3.3:24971528-24971547 None:intergenic 25.0%
! TTATCAATGGATGTTATTGT+TGG - chr3.3:24971942-24971961 MS.gene070461:CDS 25.0%
!! TTTTCGCAATGATTTATCAA+TGG - chr3.3:24971929-24971948 MS.gene070461:CDS 25.0%
AACCTATAGATTGTGATTTG+TGG - chr3.3:24972024-24972043 MS.gene070461:CDS 30.0%
AAGCGATGCTTCAAAATTTA+TGG + chr3.3:24971460-24971479 None:intergenic 30.0%
AATCACAATCTATAGGTTCA+TGG + chr3.3:24972022-24972041 None:intergenic 30.0%
ACAAAAAGTGGTGGTATTAT+TGG - chr3.3:24972200-24972219 MS.gene070461:CDS 30.0%
AGGATGGAACAATTGAAATA+TGG - chr3.3:24972081-24972100 MS.gene070461:CDS 30.0%
ATCCACAAATCACAATCTAT+AGG + chr3.3:24972029-24972048 None:intergenic 30.0%
GGATGGAACAATTGAAATAT+GGG - chr3.3:24972082-24972101 MS.gene070461:CDS 30.0%
GTTCTTCAAGCATTTATCTA+TGG - chr3.3:24971586-24971605 MS.gene070461:CDS 30.0%
TCTTTAATGGGACTATTCAT+TGG - chr3.3:24971901-24971920 MS.gene070461:CDS 30.0%
TGAATATTGGCCATCTAAAT+TGG + chr3.3:24971655-24971674 None:intergenic 30.0%
TGGAAATGTCATCAATAGAT+AGG + chr3.3:24972154-24972173 None:intergenic 30.0%
TGGAGAATTTCTAAGTCTAT+GGG - chr3.3:24972052-24972071 MS.gene070461:CDS 30.0%
TTGGAGAATTTCTAAGTCTA+TGG - chr3.3:24972051-24972070 MS.gene070461:CDS 30.0%
TTTCATTGAGAGCTAATACA+TGG - chr3.3:24971793-24971812 MS.gene070461:CDS 30.0%
! ACTTTTTGTAGAGCATATTG+GGG + chr3.3:24972189-24972208 None:intergenic 30.0%
! CATGTTGTCTGTATAGTTTT+GGG - chr3.3:24971673-24971692 MS.gene070461:CDS 30.0%
! GATTGTGATTTGTGGATATT+TGG - chr3.3:24972032-24972051 MS.gene070461:CDS 30.0%
! TCATGTTGTCTGTATAGTTT+TGG - chr3.3:24971672-24971691 MS.gene070461:CDS 30.0%
!! CACTTTTTGTAGAGCATATT+GGG + chr3.3:24972190-24972209 None:intergenic 30.0%
!! TATTGTTGGCTTTGATTTAG+TGG - chr3.3:24971956-24971975 MS.gene070461:CDS 30.0%
AATACAAAGTGCATTCGTCT+TGG - chr3.3:24972114-24972133 MS.gene070461:CDS 35.0%
AGATTAGAATAAGATTCGGG+AGG + chr3.3:24971525-24971544 None:intergenic 35.0%
AGGTATTTGTTTGTGATGTC+CGG + chr3.3:24971622-24971641 None:intergenic 35.0%
ATACAAAGTGCATTCGTCTT+GGG - chr3.3:24972115-24972134 MS.gene070461:CDS 35.0%
ATATGCTCTACAAAAAGTGG+TGG - chr3.3:24972191-24972210 MS.gene070461:CDS 35.0%
CAAATGATGATCCCAAAGTA+GGG - chr3.3:24971874-24971893 MS.gene070461:CDS 35.0%
CCAAGTAATCATCTCTTGAA+TGG + chr3.3:24971704-24971723 None:intergenic 35.0%
CCAATATGCTCTACAAAAAG+TGG - chr3.3:24972188-24972207 MS.gene070461:CDS 35.0%
CCATTCAAGAGATGATTACT+TGG - chr3.3:24971701-24971720 MS.gene070461:CDS 35.0%
GGTGGTATTATTGGAACAAA+CGG - chr3.3:24972209-24972228 MS.gene070461:CDS 35.0%
TATTGGCCATCTAAATTGGA+AGG + chr3.3:24971651-24971670 None:intergenic 35.0%
TCTAAATTGGAAGGAGGAAA+AGG + chr3.3:24971642-24971661 None:intergenic 35.0%
TGATTATACTTCACCAATCC+AGG + chr3.3:24971834-24971853 None:intergenic 35.0%
TTCAAGAGATGATTACTTGG+TGG - chr3.3:24971704-24971723 MS.gene070461:CDS 35.0%
TTTCCTCCTTCCAATTTAGA+TGG - chr3.3:24971642-24971661 MS.gene070461:CDS 35.0%
! ATTTCTAAGTCTATGGGCTA+TGG - chr3.3:24972058-24972077 MS.gene070461:CDS 35.0%
! CTTTTTGTAGAGCATATTGG+GGG + chr3.3:24972188-24972207 None:intergenic 35.0%
!! TTGGCTTTGATTTAGTGGAA+AGG - chr3.3:24971961-24971980 MS.gene070461:CDS 35.0%
!!! CCACTTTTTGTAGAGCATAT+TGG + chr3.3:24972191-24972210 None:intergenic 35.0%
AAAGTAGGGACGCTCTTTAA+TGG - chr3.3:24971888-24971907 MS.gene070461:CDS 40.0%
AAGTAGGGACGCTCTTTAAT+GGG - chr3.3:24971889-24971908 MS.gene070461:CDS 40.0%
ACTCTGTATACATAGCCACT+AGG + chr3.3:24972313-24972332 None:intergenic 40.0%
AGATATTGAGTGTCCTGGAT+TGG - chr3.3:24971818-24971837 MS.gene070461:CDS 40.0%
CTCTGTATACATAGCCACTA+GGG + chr3.3:24972312-24972331 None:intergenic 40.0%
GCAAATGATGATCCCAAAGT+AGG - chr3.3:24971873-24971892 MS.gene070461:CDS 40.0%
GGTATTATTGGAACAAACGG+TGG - chr3.3:24972212-24972231 MS.gene070461:CDS 40.0%
GTGTCCTATGATACGAGTAA+AGG - chr3.3:24971732-24971751 MS.gene070461:CDS 40.0%
TATCTATGGAATCCATCGAC+CGG - chr3.3:24971600-24971619 MS.gene070461:CDS 40.0%
TATTGGGGGATAGTACTCAA+TGG + chr3.3:24972174-24972193 None:intergenic 40.0%
TCTGTATACATAGCCACTAG+GGG + chr3.3:24972311-24972330 None:intergenic 40.0%
TGAGCCTTTACTCGTATCAT+AGG + chr3.3:24971739-24971758 None:intergenic 40.0%
TGGAAAGATATTGAGTGTCC+TGG - chr3.3:24971813-24971832 MS.gene070461:CDS 40.0%
TTGGTGAAGTATGATGGCAA+TGG - chr3.3:24972242-24972261 MS.gene070461:CDS 40.0%
! ATTCGGGAGGCATAAAATCA+AGG + chr3.3:24971512-24971531 None:intergenic 40.0%
!! ACTGGATTGGTGAAGTATGA+TGG - chr3.3:24972236-24972255 MS.gene070461:CDS 40.0%
!! ATTAAAGAGCGTCCCTACTT+TGG + chr3.3:24971889-24971908 None:intergenic 40.0%
!! TTAAAGAGCGTCCCTACTTT+GGG + chr3.3:24971888-24971907 None:intergenic 40.0%
AAGCTTGATCACTGTCACCA+GGG + chr3.3:24972349-24972368 None:intergenic 45.0%
TAAGCTTGATCACTGTCACC+AGG + chr3.3:24972350-24972369 None:intergenic 45.0%
TGGCCATCTAAATTGGAAGG+AGG + chr3.3:24971648-24971667 None:intergenic 45.0%
TGGTCAAACCCATCTGGAAA+AGG + chr3.3:24972002-24972021 None:intergenic 45.0%
! TCGAGATGCCTTTTCCAGAT+GGG - chr3.3:24971991-24972010 MS.gene070461:CDS 45.0%
! TCTAAGTCTATGGGCTATGG+AGG - chr3.3:24972061-24972080 MS.gene070461:CDS 45.0%
! TTCGAGATGCCTTTTCCAGA+TGG - chr3.3:24971990-24972009 MS.gene070461:CDS 45.0%
ATAGCCACTAGGGGTTCACA+TGG + chr3.3:24972302-24972321 None:intergenic 50.0%
ATTGGAACAAACGGTGGCAC+TGG - chr3.3:24972218-24972237 MS.gene070461:CDS 50.0%
GGTTCATGGTCAAACCCATC+TGG + chr3.3:24972008-24972027 None:intergenic 50.0%
TGTTTGTGATGTCCGGTCGA+TGG + chr3.3:24971615-24971634 None:intergenic 50.0%
! AACAAACGGTGGCACTGGAT+TGG - chr3.3:24972223-24972242 MS.gene070461:CDS 50.0%
! AGTCTATGGGCTATGGAGGA+TGG - chr3.3:24972065-24972084 MS.gene070461:CDS 50.0%
CGAACCATGTGAACCCCTAG+TGG - chr3.3:24972295-24972314 MS.gene070461:CDS 55.0%
GAGTCTCTGCTTTCACTCCC+TGG - chr3.3:24972329-24972348 MS.gene070461:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 24971429 24972370 24971429 ID=MS.gene070461
chr3.3 mRNA 24971429 24972370 24971429 ID=MS.gene070461.t1;Parent=MS.gene070461
chr3.3 exon 24971429 24972370 24971429 ID=MS.gene070461.t1.exon1;Parent=MS.gene070461.t1
chr3.3 CDS 24971429 24972370 24971429 ID=cds.MS.gene070461.t1;Parent=MS.gene070461.t1
Gene Sequence

>MS.gene070461

ATGTCAACTCTAGCTCTCGAAACTCGATCCATAAATTTTGAAGCATCGCTTAACGATGATTCTGCTTCTACTTCACTGAACCTTGATTTTATGCCTCCCGAATCTTATTCTAATCTTGAAATTAAAAGTTCATGTAGAGGATTTATAGTTTTGACTTGTTCTTCAAGCATTTATCTATGGAATCCATCGACCGGACATCACAAACAAATACCTTTTCCTCCTTCCAATTTAGATGGCCAATATTCATGTTGTCTGTATAGTTTTGGGTACGACCATTCAAGAGATGATTACTTGGTGGTATCAGTGTCCTATGATACGAGTAAAGGCTCAATAGAAGATAATATTTCATCACATTTGGAGTTTTTTTCATTGAGAGCTAATACATGGAAAGATATTGAGTGTCCTGGATTGGTGAAGTATAATCACTTCCCTTATTATATGAATGCAAATGATGATCCCAAAGTAGGGACGCTCTTTAATGGGACTATTCATTGGTTCTCTTTTCGCAATGATTTATCAATGGATGTTATTGTTGGCTTTGATTTAGTGGAAAGGAAATTTTTCGAGATGCCTTTTCCAGATGGGTTTGACCATGAACCTATAGATTGTGATTTGTGGATATTTGGAGAATTTCTAAGTCTATGGGCTATGGAGGATGGAACAATTGAAATATGGGTTATGAAAGAATACAAAGTGCATTCGTCTTGGGTTGAAACTCTTGTCCTATCTATTGATGACATTTCCATTGAGTACTATCCCCCAATATGCTCTACAAAAAGTGGTGGTATTATTGGAACAAACGGTGGCACTGGATTGGTGAAGTATGATGGCAATGGTCAGTTGCTAGAGAATCGCTCATACTATAACGAACCATGTGAACCCCTAGTGGCTATGTATACAGAGTCTCTGCTTTCACTCCCTGGTGACAGTGATCAAGCTTAA
Protein sequence

>MS.gene070461.t1

MSTLALETRSINFEASLNDDSASTSLNLDFMPPESYSNLEIKSSCRGFIVLTCSSSIYLWNPSTGHHKQIPFPPSNLDGQYSCCLYSFGYDHSRDDYLVVSVSYDTSKGSIEDNISSHLEFFSLRANTWKDIECPGLVKYNHFPYYMNANDDPKVGTLFNGTIHWFSFRNDLSMDVIVGFDLVERKFFEMPFPDGFDHEPIDCDLWIFGEFLSLWAMEDGTIEIWVMKEYKVHSSWVETLVLSIDDISIEYYPPICSTKSGGIIGTNGGTGLVKYDGNGQLLENRSYYNEPCEPLVAMYTESLLSLPGDSDQA