AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26830


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26830.t1 MTR_3g103020 61.176 170 62 3 1 167 210 378 5.15e-65 203
MS.gene26830.t1 MTR_3g034180 60.355 169 62 3 1 167 324 489 4.00e-62 199
MS.gene26830.t1 MTR_4g116320 57.576 165 56 4 6 162 95 253 9.93e-58 181
MS.gene26830.t1 MTR_4g109270 60.248 161 60 3 10 167 205 364 1.02e-57 184
MS.gene26830.t1 MTR_6g060650 57.396 169 66 4 1 166 207 372 7.61e-57 182
MS.gene26830.t1 MTR_4g116330 56.805 169 67 4 4 167 213 380 6.13e-56 180
MS.gene26830.t1 MTR_3g034130 58.333 156 59 4 1 153 210 362 2.62e-54 176
MS.gene26830.t1 MTR_4g117720 56.977 172 62 6 1 163 205 373 2.65e-54 176
MS.gene26830.t1 MTR_4g122840 67.669 133 11 1 35 167 164 264 3.50e-54 172
MS.gene26830.t1 MTR_4g109280 58.025 162 61 4 10 167 201 359 1.62e-53 173
MS.gene26830.t1 MTR_4g023090 51.705 176 67 3 7 166 210 383 3.15e-53 173
MS.gene26830.t1 MTR_4g023130 60.000 160 57 5 10 166 157 312 4.85e-53 171
MS.gene26830.t1 MTR_8g040935 56.970 165 63 5 10 167 45 208 1.12e-52 167
MS.gene26830.t1 MTR_8g433030 56.548 168 65 6 1 163 174 338 2.78e-52 169
MS.gene26830.t1 MTR_3g034160 56.805 169 66 5 1 166 210 374 3.03e-52 171
MS.gene26830.t1 MTR_4g023080 58.750 160 59 5 10 166 198 353 3.27e-52 170
MS.gene26830.t1 MTR_8g032390 56.548 168 65 6 1 163 207 371 6.04e-52 169
MS.gene26830.t1 MTR_4g122850 58.182 165 63 6 7 167 202 364 2.47e-51 168
MS.gene26830.t1 MTR_6g463430 53.846 169 73 3 1 167 37 202 8.47e-51 162
MS.gene26830.t1 MTR_4g023070 54.913 173 59 5 10 166 200 369 1.59e-50 166
MS.gene26830.t1 MTR_4g117850 57.595 158 59 5 10 160 217 373 3.46e-50 165
MS.gene26830.t1 MTR_4g023380 50.802 187 64 5 7 167 195 379 3.18e-49 163
MS.gene26830.t1 MTR_5g097210 55.696 158 65 4 7 162 204 358 6.07e-48 159
MS.gene26830.t1 MTR_5g070080 55.844 154 65 2 12 163 229 381 6.24e-48 159
MS.gene26830.t1 MTR_4g094365 54.938 162 70 3 9 167 203 364 6.83e-48 159
MS.gene26830.t1 MTR_4g023120 54.023 174 61 6 10 167 200 370 1.78e-47 158
MS.gene26830.t1 MTR_5g070130 53.846 156 67 4 12 163 218 372 2.02e-46 155
MS.gene26830.t1 MTR_4g021785 55.625 160 64 5 10 166 199 354 2.08e-46 155
MS.gene26830.t1 MTR_4g023190 53.374 163 66 5 10 165 198 357 1.93e-45 152
MS.gene26830.t1 MTR_4g117780 54.268 164 70 3 9 167 204 367 4.80e-45 152
MS.gene26830.t1 MTR_4g117770 53.939 165 71 3 8 167 203 367 9.19e-45 151
MS.gene26830.t1 MTR_4g117810 53.939 165 71 3 8 167 203 367 9.19e-45 151
MS.gene26830.t1 MTR_4g023150 52.761 163 67 5 10 165 199 358 9.57e-45 150
MS.gene26830.t1 MTR_4g023200 50.920 163 70 5 10 165 199 358 7.76e-44 148
MS.gene26830.t1 MTR_4g118380 56.643 143 49 4 9 140 217 357 2.62e-43 147
MS.gene26830.t1 MTR_8g433040 52.976 168 64 5 2 167 210 364 2.82e-43 147
MS.gene26830.t1 MTR_8g032380 52.976 168 64 5 2 167 210 364 2.82e-43 147
MS.gene26830.t1 MTR_1g055175 49.367 158 73 6 13 166 46 200 1.32e-40 135
MS.gene26830.t1 MTR_1g055135 49.057 159 75 4 13 166 217 374 2.64e-40 139
MS.gene26830.t1 MTR_5g070088 53.595 153 66 5 15 163 230 381 6.15e-40 139
MS.gene26830.t1 MTR_1g055165 49.367 158 73 6 13 166 163 317 4.97e-39 135
MS.gene26830.t1 MTR_1g055195 50.327 153 70 4 12 161 323 472 1.03e-38 137
MS.gene26830.t1 MTR_4g118400 48.780 164 66 4 1 161 146 294 3.30e-38 132
MS.gene26830.t1 MTR_4g117870 55.814 129 53 4 41 167 141 267 5.30e-37 128
MS.gene26830.t1 MTR_7g079400 45.783 166 81 5 9 167 174 337 9.29e-37 129
MS.gene26830.t1 MTR_7g079370 47.205 161 81 3 9 167 198 356 1.04e-36 130
MS.gene26830.t1 MTR_4g117530 44.099 161 53 4 10 167 127 253 1.36e-34 122
MS.gene26830.t1 MTR_4g117750 48.171 164 72 4 12 166 199 358 1.92e-34 126
MS.gene26830.t1 MTR_4g117750 55.660 106 44 2 60 162 373 478 2.17e-27 107
MS.gene26830.t1 MTR_4g118370 46.154 156 59 5 12 164 124 257 3.57e-33 118
MS.gene26830.t1 MTR_4g023440 44.720 161 46 4 10 167 212 332 1.81e-32 118
MS.gene26830.t1 MTR_4g118360 53.636 110 46 3 55 161 13 120 1.11e-31 110
MS.gene26830.t1 MTR_8g102520 42.593 162 88 4 9 167 163 322 1.13e-31 115
MS.gene26830.t1 MTR_5g023000 56.566 99 40 2 72 167 1 99 1.70e-31 109
MS.gene26830.t1 MTR_1g055250 46.565 131 66 4 13 142 116 243 2.87e-31 115
MS.gene26830.t1 MTR_4g116330 41.420 169 54 4 4 167 213 341 8.36e-30 111
MS.gene26830.t1 MTR_7g079420 42.073 164 85 7 9 166 984 1143 4.04e-28 109
MS.gene26830.t1 MTR_4g122860 41.040 173 70 5 1 167 174 320 3.07e-27 104
MS.gene26830.t1 MTR_8g098830 43.077 130 71 2 9 137 68 195 8.17e-27 101
MS.gene26830.t1 MTR_4g121580 43.671 158 57 6 1 157 213 339 6.94e-26 101
MS.gene26830.t1 MTR_7g079450 41.497 147 69 5 28 167 1 137 1.87e-24 92.8
MS.gene26830.t1 MTR_7g117720 59.770 87 28 3 83 162 186 272 6.16e-22 89.4
MS.gene26830.t1 MTR_2g048580 36.196 163 89 5 9 167 191 342 2.42e-21 89.0
MS.gene26830.t1 MTR_4g023430 57.317 82 29 3 92 167 141 222 1.04e-20 85.1
MS.gene26830.t1 MTR_4g114700 50.526 95 41 3 70 158 124 218 2.40e-18 79.3
MS.gene26830.t1 MTR_4g117430 61.905 63 23 1 10 72 94 155 1.23e-17 75.5
MS.gene26830.t1 MTR_8g013010 57.971 69 24 1 1 69 246 309 1.46e-17 78.2
MS.gene26830.t1 MTR_8g013005 68.519 54 16 1 115 167 6 59 1.45e-16 70.1
MS.gene26830.t1 MTR_2g064960 64.286 42 15 0 126 167 18 59 4.55e-11 55.8
MS.gene26830.t1 MTR_4g109290 62.264 53 19 1 116 167 136 188 5.80e-11 58.5
MS.gene26830.t1 MTR_2g046830 27.206 136 90 2 10 137 220 354 9.13e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGTTGCCTTTGATTTAA+CGG 0.303490 8.1:-8059556 MS.gene26830:CDS
TGGAGAATTTCTTAGTTTAT+CGG 0.313818 8.1:-8059457 MS.gene26830:CDS
GAGTCTCTACTTTCACTTCC+TGG 0.326582 8.1:-8059183 MS.gene26830:CDS
TCTTTACCGGGGCTATTCAT+TGG 0.344445 8.1:-8059611 MS.gene26830:CDS
TGAGTGATACAATTGAATTA+TGG 0.355506 8.1:-8059422 MS.gene26830:CDS
GGTGATATCATTGGAACAAA+TGG 0.406561 8.1:-8059300 MS.gene26830:CDS
GGCTTATCATCATGATTTAT+CGG 0.407804 8.1:-8059586 MS.gene26830:CDS
AATTGTGATTTGTGGGTGTC+TGG 0.408395 8.1:-8059477 MS.gene26830:CDS
GTGGTGAAGTACAATTATAA+AGG 0.417869 8.1:-8059270 MS.gene26830:CDS
TACCGGGGCTATTCATTGGT+TGG 0.429346 8.1:-8059607 MS.gene26830:CDS
ACCCACAAATCACAATTTAT+AGG 0.436272 8.1:+8059483 None:intergenic
GCTTCGAGTGATCGCACTCT+AGG 0.437226 8.1:-8059639 MS.gene26830:CDS
GAGTGATACAATTGAATTAT+GGG 0.441328 8.1:-8059421 MS.gene26830:CDS
TGGTGAAGTACAATTATAAA+GGG 0.476779 8.1:-8059269 MS.gene26830:CDS
TTTCCTTTCCGTTAAATCAA+AGG 0.478785 8.1:+8059548 None:intergenic
TACCTATAAATTGTGATTTG+TGG 0.478856 8.1:-8059485 MS.gene26830:CDS
ACCTATAAATTGTGATTTGT+GGG 0.488234 8.1:-8059484 MS.gene26830:CDS
ATGACCACTTGGGATCCATC+TGG 0.498070 8.1:+8059213 None:intergenic
AATACAAAGTCAATTCGTCT+TGG 0.507033 8.1:-8059389 MS.gene26830:CDS
ACATGGACAAAATCACCATC+TGG 0.527594 8.1:+8059507 None:intergenic
TAAACTTGCTCAGCGCCACC+AGG 0.536422 8.1:+8059165 None:intergenic
CTTCGAGTGATCGCACTCTA+GGG 0.546915 8.1:-8059638 MS.gene26830:CDS
TTAGAGATGCCTCTTCCAGA+TGG 0.562110 8.1:-8059522 MS.gene26830:CDS
TTGCCTTTGATTTAACGGAA+AGG 0.569675 8.1:-8059551 MS.gene26830:CDS
AGCCAACCAATGAATAGCCC+CGG 0.580401 8.1:+8059605 None:intergenic
ACTCTGTATACATGACCACT+TGG 0.585275 8.1:+8059202 None:intergenic
GATATCATTGGAACAAATGG+AGG 0.586188 8.1:-8059297 MS.gene26830:CDS
ACAAAATCACCATCTGGAAG+AGG 0.587693 8.1:+8059513 None:intergenic
AATCACAATTTATAGGTACA+TGG 0.595399 8.1:+8059490 None:intergenic
TCTTATTCTGATGATCCAGA+TGG 0.622390 8.1:-8059228 MS.gene26830:CDS
CTCTGTATACATGACCACTT+GGG 0.622804 8.1:+8059203 None:intergenic
TTATCATCATGATTTATCGG+TGG 0.672751 8.1:-8059583 MS.gene26830:CDS
TGGAACAAATGGAGGTAATG+TGG 0.673690 8.1:-8059289 MS.gene26830:CDS
TCTCTACTTTCACTTCCTGG+TGG 0.686381 8.1:-8059180 MS.gene26830:CDS
TGATCCAGATGGATCCCAAG+TGG 0.718470 8.1:-8059217 MS.gene26830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATCACAATTTATAGGTACA+TGG + chr8.1:8059322-8059341 None:intergenic 25.0%
! GAGTGATACAATTGAATTAT+GGG - chr8.1:8059388-8059407 MS.gene26830:CDS 25.0%
! TACCTATAAATTGTGATTTG+TGG - chr8.1:8059324-8059343 MS.gene26830:CDS 25.0%
! TGAGTGATACAATTGAATTA+TGG - chr8.1:8059387-8059406 MS.gene26830:CDS 25.0%
! TGGAGAATTTCTTAGTTTAT+CGG - chr8.1:8059352-8059371 MS.gene26830:CDS 25.0%
! TGGTGAAGTACAATTATAAA+GGG - chr8.1:8059540-8059559 MS.gene26830:CDS 25.0%
!! ACCTATAAATTGTGATTTGT+GGG - chr8.1:8059325-8059344 MS.gene26830:CDS 25.0%
!! TATTGTTGCCTTTGATTTAA+CGG - chr8.1:8059253-8059272 MS.gene26830:CDS 25.0%
AATACAAAGTCAATTCGTCT+TGG - chr8.1:8059420-8059439 MS.gene26830:CDS 30.0%
ACCCACAAATCACAATTTAT+AGG + chr8.1:8059329-8059348 None:intergenic 30.0%
CCTATGTACTTTGCAAAAAA+CGG - chr8.1:8059488-8059507 MS.gene26830:CDS 30.0%
GGCTTATCATCATGATTTAT+CGG - chr8.1:8059223-8059242 MS.gene26830:CDS 30.0%
GTGGTGAAGTACAATTATAA+AGG - chr8.1:8059539-8059558 MS.gene26830:CDS 30.0%
TTATCATCATGATTTATCGG+TGG - chr8.1:8059226-8059245 MS.gene26830:CDS 30.0%
TTTCCTTTCCGTTAAATCAA+AGG + chr8.1:8059264-8059283 None:intergenic 30.0%
!!! CGTTTTTTGCAAAGTACATA+GGG + chr8.1:8059490-8059509 None:intergenic 30.0%
!!! GTTTTTTGCAAAGTACATAG+GGG + chr8.1:8059489-8059508 None:intergenic 30.0%
GATATCATTGGAACAAATGG+AGG - chr8.1:8059512-8059531 MS.gene26830:CDS 35.0%
GCAAAAAACGGTGATATCAT+TGG - chr8.1:8059500-8059519 MS.gene26830:CDS 35.0%
GGTGATATCATTGGAACAAA+TGG - chr8.1:8059509-8059528 MS.gene26830:CDS 35.0%
TCTTATTCTGATGATCCAGA+TGG - chr8.1:8059581-8059600 MS.gene26830:CDS 35.0%
! TTGCCTTTGATTTAACGGAA+AGG - chr8.1:8059258-8059277 MS.gene26830:CDS 35.0%
!!! CCGTTTTTTGCAAAGTACAT+AGG + chr8.1:8059491-8059510 None:intergenic 35.0%
ACAAAATCACCATCTGGAAG+AGG + chr8.1:8059299-8059318 None:intergenic 40.0%
ACATGGACAAAATCACCATC+TGG + chr8.1:8059305-8059324 None:intergenic 40.0%
ACTCTGTATACATGACCACT+TGG + chr8.1:8059610-8059629 None:intergenic 40.0%
CTCTGTATACATGACCACTT+GGG + chr8.1:8059609-8059628 None:intergenic 40.0%
TGGAACAAATGGAGGTAATG+TGG - chr8.1:8059520-8059539 MS.gene26830:CDS 40.0%
! AATTGTGATTTGTGGGTGTC+TGG - chr8.1:8059332-8059351 MS.gene26830:CDS 40.0%
!!! ACTCTAGGGCTTTTCTTTAC+CGG - chr8.1:8059185-8059204 MS.gene26830:CDS 40.0%
GAGTCTCTACTTTCACTTCC+TGG - chr8.1:8059626-8059645 MS.gene26830:CDS 45.0%
TCTCTACTTTCACTTCCTGG+TGG - chr8.1:8059629-8059648 MS.gene26830:CDS 45.0%
TCTTTACCGGGGCTATTCAT+TGG - chr8.1:8059198-8059217 MS.gene26830:CDS 45.0%
TTAGAGATGCCTCTTCCAGA+TGG - chr8.1:8059287-8059306 MS.gene26830:CDS 45.0%
!! TCTAGGGCTTTTCTTTACCG+GGG - chr8.1:8059187-8059206 MS.gene26830:CDS 45.0%
!!! CTCTAGGGCTTTTCTTTACC+GGG - chr8.1:8059186-8059205 MS.gene26830:CDS 45.0%
AGCCAACCAATGAATAGCCC+CGG + chr8.1:8059207-8059226 None:intergenic 50.0%
ATGACCACTTGGGATCCATC+TGG + chr8.1:8059599-8059618 None:intergenic 50.0%
CTTCGAGTGATCGCACTCTA+GGG - chr8.1:8059171-8059190 MS.gene26830:CDS 50.0%
TACCGGGGCTATTCATTGGT+TGG - chr8.1:8059202-8059221 MS.gene26830:CDS 50.0%
TGATCCAGATGGATCCCAAG+TGG - chr8.1:8059592-8059611 MS.gene26830:CDS 50.0%
GCTTCGAGTGATCGCACTCT+AGG - chr8.1:8059170-8059189 MS.gene26830:CDS 55.0%
! TAAACTTGCTCAGCGCCACC+AGG + chr8.1:8059647-8059666 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 8059164 8059667 8059164 ID=MS.gene26830
chr8.1 mRNA 8059164 8059667 8059164 ID=MS.gene26830.t1;Parent=MS.gene26830
chr8.1 exon 8059164 8059667 8059164 ID=MS.gene26830.t1.exon1;Parent=MS.gene26830.t1
chr8.1 CDS 8059164 8059667 8059164 ID=cds.MS.gene26830.t1;Parent=MS.gene26830.t1
Gene Sequence

>MS.gene26830

ATGGATGCTTCGAGTGATCGCACTCTAGGGCTTTTCTTTACCGGGGCTATTCATTGGTTGGCTTATCATCATGATTTATCGGTGGATGTTATTGTTGCCTTTGATTTAACGGAAAGGAAACTTTTAGAGATGCCTCTTCCAGATGGTGATTTTGTCCATGTACCTATAAATTGTGATTTGTGGGTGTCTGGAGAATTTCTTAGTTTATCGGCTATTGATTATGTGAGTGATACAATTGAATTATGGGTGATGAAAGAATACAAAGTCAATTCGTCTTGGACTAAAACTCTTGTTATTCACATTGATAATGTCGCTTACTTTTCCCCTATGTACTTTGCAAAAAACGGTGATATCATTGGAACAAATGGAGGTAATGTGGTGAAGTACAATTATAAAGGGCAGTTGTTAGATCATCGCTCTTATTCTGATGATCCAGATGGATCCCAAGTGGTCATGTATACAGAGTCTCTACTTTCACTTCCTGGTGGCGCTGAGCAAGTTTAA

Protein sequence

>MS.gene26830.t1

MDASSDRTLGLFFTGAIHWLAYHHDLSVDVIVAFDLTERKLLEMPLPDGDFVHVPINCDLWVSGEFLSLSAIDYVSDTIELWVMKEYKVNSSWTKTLVIHIDNVAYFSPMYFAKNGDIIGTNGGNVVKYNYKGQLLDHRSYSDDPDGSQVVMYTESLLSLPGGAEQV