AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035236


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035236.t1 MTR_3g103020 60.804 199 75 1 8 203 180 378 8.09e-80 243
MS.gene035236.t1 MTR_6g060650 59.794 194 76 1 9 202 181 372 1.74e-79 242
MS.gene035236.t1 MTR_4g117720 60.294 204 72 4 7 203 177 378 1.76e-78 239
MS.gene035236.t1 MTR_4g109280 61.616 198 70 4 9 203 165 359 1.05e-76 234
MS.gene035236.t1 MTR_4g116320 60.209 191 72 2 10 198 65 253 2.75e-71 217
MS.gene035236.t1 MTR_4g116330 56.771 192 81 1 14 203 189 380 1.87e-69 216
MS.gene035236.t1 MTR_4g109270 57.732 194 80 2 12 203 171 364 4.19e-69 215
MS.gene035236.t1 MTR_8g040935 56.436 202 83 3 7 203 7 208 1.77e-68 208
MS.gene035236.t1 MTR_4g094365 56.410 195 82 2 11 203 171 364 8.05e-67 209
MS.gene035236.t1 MTR_3g034180 56.219 201 78 4 9 202 291 488 7.64e-66 210
MS.gene035236.t1 MTR_4g023130 58.081 198 78 4 6 202 119 312 1.53e-64 202
MS.gene035236.t1 MTR_4g122850 54.455 202 85 6 7 203 165 364 1.71e-64 204
MS.gene035236.t1 MTR_4g117850 55.779 199 83 3 3 196 175 373 1.45e-63 201
MS.gene035236.t1 MTR_4g023080 56.566 198 81 4 6 202 160 353 1.22e-62 198
MS.gene035236.t1 MTR_3g034130 54.787 188 76 3 9 189 177 362 4.68e-62 197
MS.gene035236.t1 MTR_4g118380 56.497 177 66 2 9 176 183 357 1.28e-61 196
MS.gene035236.t1 MTR_6g463430 54.688 192 80 3 14 203 16 202 2.56e-61 190
MS.gene035236.t1 MTR_3g034160 54.726 201 81 4 9 202 177 374 2.99e-61 195
MS.gene035236.t1 MTR_8g433030 57.360 197 76 6 7 199 146 338 3.40e-61 194
MS.gene035236.t1 MTR_8g032390 57.360 197 76 6 7 199 179 371 1.14e-60 194
MS.gene035236.t1 MTR_4g021785 54.822 197 85 3 8 203 162 355 1.57e-60 192
MS.gene035236.t1 MTR_4g023090 50.242 207 86 4 10 202 180 383 1.75e-59 191
MS.gene035236.t1 MTR_8g433040 54.500 200 75 4 7 203 178 364 8.62e-59 189
MS.gene035236.t1 MTR_8g032380 54.500 200 75 4 7 203 178 364 8.62e-59 189
MS.gene035236.t1 MTR_4g118400 53.968 189 73 3 9 197 120 294 1.76e-58 186
MS.gene035236.t1 MTR_4g023380 49.115 226 86 6 2 203 159 379 8.10e-58 186
MS.gene035236.t1 MTR_4g117780 51.613 217 90 3 2 203 151 367 2.85e-57 185
MS.gene035236.t1 MTR_4g117770 52.074 217 89 3 2 203 151 367 7.76e-57 184
MS.gene035236.t1 MTR_4g117810 52.074 217 89 3 2 203 151 367 7.76e-57 184
MS.gene035236.t1 MTR_4g023070 51.220 205 84 4 11 202 168 369 8.82e-57 184
MS.gene035236.t1 MTR_4g023120 50.971 206 85 4 11 203 168 370 2.37e-56 182
MS.gene035236.t1 MTR_5g097210 53.608 194 82 5 9 198 169 358 6.74e-56 181
MS.gene035236.t1 MTR_4g023190 49.296 213 91 6 2 201 149 357 4.55e-52 171
MS.gene035236.t1 MTR_4g117870 48.000 200 68 4 4 203 104 267 1.79e-51 167
MS.gene035236.t1 MTR_5g070080 50.515 194 93 3 12 203 194 386 2.61e-51 170
MS.gene035236.t1 MTR_1g055175 48.205 195 94 5 14 203 9 201 1.43e-50 162
MS.gene035236.t1 MTR_5g070130 47.805 205 97 4 9 203 173 377 1.21e-49 166
MS.gene035236.t1 MTR_4g023150 47.418 213 96 6 2 201 149 358 5.81e-49 163
MS.gene035236.t1 MTR_4g118370 46.429 196 80 5 9 200 83 257 2.90e-48 158
MS.gene035236.t1 MTR_4g023200 46.009 213 99 6 2 201 149 358 4.44e-48 160
MS.gene035236.t1 MTR_7g079400 47.030 202 90 8 14 203 141 337 1.92e-46 156
MS.gene035236.t1 MTR_5g070088 46.316 190 100 2 12 199 192 381 7.12e-46 155
MS.gene035236.t1 MTR_1g055195 47.000 200 92 5 14 203 283 478 7.37e-46 158
MS.gene035236.t1 MTR_4g117750 46.269 201 93 5 9 198 158 354 2.98e-45 156
MS.gene035236.t1 MTR_4g117750 56.061 132 50 1 78 201 350 481 3.32e-40 143
MS.gene035236.t1 MTR_4g116330 44.792 192 65 2 14 203 189 341 3.28e-44 150
MS.gene035236.t1 MTR_8g102520 42.381 210 108 7 2 203 118 322 1.58e-43 148
MS.gene035236.t1 MTR_1g055135 43.478 207 99 7 9 203 175 375 5.13e-43 148
MS.gene035236.t1 MTR_1g055165 50.000 164 79 3 41 203 157 318 6.10e-43 146
MS.gene035236.t1 MTR_4g117530 40.758 211 83 4 2 203 76 253 1.27e-42 144
MS.gene035236.t1 MTR_7g079370 45.146 206 90 11 4 197 156 350 2.10e-41 143
MS.gene035236.t1 MTR_4g122860 41.579 190 80 5 19 203 157 320 1.62e-40 140
MS.gene035236.t1 MTR_4g023440 43.523 193 69 4 11 203 180 332 7.17e-40 139
MS.gene035236.t1 MTR_1g055250 42.632 190 97 6 9 193 73 255 1.07e-37 133
MS.gene035236.t1 MTR_4g118360 56.800 125 44 5 78 197 1 120 1.15e-37 127
MS.gene035236.t1 MTR_8g098830 44.509 173 85 6 6 172 27 194 3.14e-37 129
MS.gene035236.t1 MTR_7g079420 41.081 185 93 7 29 203 966 1144 1.38e-36 135
MS.gene035236.t1 MTR_2g048580 40.201 199 96 8 14 203 158 342 1.64e-36 130
MS.gene035236.t1 MTR_5g023000 60.606 99 39 0 105 203 1 99 1.37e-34 118
MS.gene035236.t1 MTR_4g114700 40.426 188 59 3 12 194 79 218 2.68e-32 117
MS.gene035236.t1 MTR_4g121580 45.455 165 56 6 11 170 183 318 7.82e-31 116
MS.gene035236.t1 MTR_7g079450 46.479 142 67 5 64 203 3 137 1.06e-30 109
MS.gene035236.t1 MTR_8g013010 58.333 96 36 1 7 102 218 309 3.18e-30 113
MS.gene035236.t1 MTR_4g117430 55.789 95 39 2 12 105 63 155 2.27e-25 96.7
MS.gene035236.t1 MTR_4g122840 44.118 136 40 3 69 203 164 264 4.47e-23 93.6
MS.gene035236.t1 MTR_7g117720 59.770 87 31 2 116 198 186 272 3.45e-22 91.3
MS.gene035236.t1 MTR_8g013005 69.492 59 18 0 145 203 1 59 1.77e-21 84.0
MS.gene035236.t1 MTR_4g023430 52.439 82 36 1 125 203 141 222 1.97e-18 80.1
MS.gene035236.t1 MTR_4g116340 53.226 62 29 0 7 68 186 247 2.23e-15 72.4
MS.gene035236.t1 MTR_6g034720 41.333 75 44 0 4 78 94 168 6.80e-13 65.1
MS.gene035236.t1 MTR_2g064960 66.667 42 14 0 162 203 18 59 4.06e-12 59.3
MS.gene035236.t1 MTR_4g109290 62.264 53 20 0 151 203 136 188 7.80e-12 62.0
MS.gene035236.t1 MTR_4g023100 58.929 56 21 1 147 202 65 118 4.15e-11 58.5
MS.gene035236.t1 MTR_2g426020 24.868 189 129 5 13 199 190 367 7.12e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCCTACATATTGTGATTTA+TGG 0.083398 8.1:-9571249 MS.gene035236:CDS
TATTGTTGCCTTTGATTTAA+TGG 0.144282 8.1:-9571317 MS.gene035236:CDS
TATTGTGATTTATGGGTATT+TGG 0.179333 8.1:-9571241 MS.gene035236:CDS
ATAATACTACAATTGAAATA+TGG 0.246667 8.1:-9571186 MS.gene035236:CDS
TTATCAGAGTCTTTCATATA+AGG 0.262208 8.1:+9571412 None:intergenic
TCTCATTGAGAGATAATAAA+TGG 0.282861 8.1:-9571459 MS.gene035236:CDS
CATAACTCCTTCTATTACTA+TGG 0.288204 8.1:-9570989 MS.gene035236:CDS
TACTCGGTAGTTTCTATGTT+TGG 0.315315 8.1:-9571523 None:intergenic
TCCTACATATTGTGATTTAT+GGG 0.345705 8.1:-9571248 MS.gene035236:CDS
CTCTGTATACATAGCAAATT+CGG 0.366110 8.1:+9570958 None:intergenic
TTGCTCAAGTATAATGATAA+AGG 0.368445 8.1:-9571025 MS.gene035236:CDS
ATTATAGAACAACCCTGCTC+TGG 0.379741 8.1:+9571387 None:intergenic
TATCAGAGTCTTTCATATAA+GGG 0.414042 8.1:+9571413 None:intergenic
TAATACTACAATTGAAATAT+GGG 0.415497 8.1:-9571185 MS.gene035236:CDS
TCCATGGGAATGTCATTAAT+AGG 0.437110 8.1:+9571112 None:intergenic
GACTCTGATAAGCCCAGAGC+AGG 0.444411 8.1:-9571400 MS.gene035236:CDS
TTAAGCTTGCTCACTGGCAC+CGG 0.451608 8.1:+9570919 None:intergenic
TCCACGTCCATAGTAATAGA+AGG 0.458037 8.1:+9570982 None:intergenic
TAATACCGCTGTTCATTGGT+TGG 0.464430 8.1:-9571368 MS.gene035236:CDS
GTGATGAAATATCAGCTAAT+CGG 0.476782 8.1:+9571494 None:intergenic
TCTATAATACCGCTGTTCAT+TGG 0.478155 8.1:-9571372 MS.gene035236:CDS
TTTCATTTCCATTAAATCAA+AGG 0.478785 8.1:+9571309 None:intergenic
TAAAGGGATAAAGTATTCCA+TGG 0.494497 8.1:+9571096 None:intergenic
TAAGCTTGCTCACTGGCACC+GGG 0.497952 8.1:+9570920 None:intergenic
GTGATATTATTGTAACGGAC+GGG 0.507765 8.1:-9571057 MS.gene035236:CDS
AATCACAATATGTAGGATCA+TGG 0.508999 8.1:+9571254 None:intergenic
GAGTCTCTGCTTTCACTCCC+CGG 0.510671 8.1:-9570938 MS.gene035236:CDS
AATACACAGTGCATTCGTCT+TGG 0.518219 8.1:-9571153 MS.gene035236:CDS
TCCTATTAATGACATTCCCA+TGG 0.530486 8.1:-9571113 MS.gene035236:CDS
ATCCTCTTAAGCTTGCTCAC+TGG 0.558265 8.1:+9570913 None:intergenic
TTATAGAACAACCCTGCTCT+GGG 0.560523 8.1:+9571388 None:intergenic
TCCTTCTATTACTATGGACG+TGG 0.563067 8.1:-9570983 MS.gene035236:CDS
ACCCATAAATCACAATATGT+AGG 0.563770 8.1:+9571247 None:intergenic
AAGTGGTGATATTATTGTAA+CGG 0.572882 8.1:-9571062 MS.gene035236:CDS
TGATATTATTGTAACGGACG+GGG 0.588308 8.1:-9571056 MS.gene035236:CDS
AAAGGGATAAAGTATTCCAT+GGG 0.621594 8.1:+9571097 None:intergenic
GGTGATATTATTGTAACGGA+CGG 0.635845 8.1:-9571058 MS.gene035236:CDS
AAGCTTGCTCACTGGCACCG+GGG 0.669724 8.1:+9570921 None:intergenic
GATATTATTGTAACGGACGG+GGG 0.685777 8.1:-9571055 MS.gene035236:CDS
ACTCTGATAAGCCCAGAGCA+GGG 0.688677 8.1:-9571399 MS.gene035236:CDS
AAAAGCCAACCAATGAACAG+CGG 0.744255 8.1:+9571363 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATACTACAATTGAAATA+TGG - chr8.1:9571241-9571260 MS.gene035236:CDS 15.0%
!! TAATACTACAATTGAAATAT+GGG - chr8.1:9571242-9571261 MS.gene035236:CDS 15.0%
!!! TTTTAATGATGATTTAACAT+TGG - chr8.1:9571083-9571102 MS.gene035236:CDS 15.0%
!! AATAAATGGAGAAAAATTGA+GGG - chr8.1:9570982-9571001 MS.gene035236:CDS 20.0%
!! TAATAAATGGAGAAAAATTG+AGG - chr8.1:9570981-9571000 MS.gene035236:CDS 20.0%
!! TTTCATTTCCATTAAATCAA+AGG + chr8.1:9571121-9571140 None:intergenic 20.0%
! AAGTGGTGATATTATTGTAA+CGG - chr8.1:9571365-9571384 MS.gene035236:CDS 25.0%
! ATCCTACATATTGTGATTTA+TGG - chr8.1:9571178-9571197 MS.gene035236:CDS 25.0%
! TATCAGAGTCTTTCATATAA+GGG + chr8.1:9571017-9571036 None:intergenic 25.0%
! TATTGTGATTTATGGGTATT+TGG - chr8.1:9571186-9571205 MS.gene035236:CDS 25.0%
! TCCTACATATTGTGATTTAT+GGG - chr8.1:9571179-9571198 MS.gene035236:CDS 25.0%
! TCTCATTGAGAGATAATAAA+TGG - chr8.1:9570968-9570987 MS.gene035236:CDS 25.0%
! TTATCAGAGTCTTTCATATA+AGG + chr8.1:9571018-9571037 None:intergenic 25.0%
! TTGCTCAAGTATAATGATAA+AGG - chr8.1:9571402-9571421 MS.gene035236:CDS 25.0%
!! TATTGTTGCCTTTGATTTAA+TGG - chr8.1:9571110-9571129 MS.gene035236:CDS 25.0%
AATCACAATATGTAGGATCA+TGG + chr8.1:9571176-9571195 None:intergenic 30.0%
ACCCATAAATCACAATATGT+AGG + chr8.1:9571183-9571202 None:intergenic 30.0%
CATAACTCCTTCTATTACTA+TGG - chr8.1:9571438-9571457 MS.gene035236:CDS 30.0%
CTCTGTATACATAGCAAATT+CGG + chr8.1:9571472-9571491 None:intergenic 30.0%
GTGATGAAATATCAGCTAAT+CGG + chr8.1:9570936-9570955 None:intergenic 30.0%
! AAAGGGATAAAGTATTCCAT+GGG + chr8.1:9571333-9571352 None:intergenic 30.0%
! TAAAGGGATAAAGTATTCCA+TGG + chr8.1:9571334-9571353 None:intergenic 30.0%
! TGGAGTTTTTCTTAGTCTAT+GGG - chr8.1:9571206-9571225 MS.gene035236:CDS 30.0%
! TTGGAGTTTTTCTTAGTCTA+TGG - chr8.1:9571205-9571224 MS.gene035236:CDS 30.0%
!! CACTTTTTGTTGAGCATAAA+GGG + chr8.1:9571350-9571369 None:intergenic 30.0%
CCTTTATGCTCAACAAAAAG+TGG - chr8.1:9571348-9571367 MS.gene035236:CDS 35.0%
GGATCATGGTAAAAATCATC+CGG + chr8.1:9571162-9571181 None:intergenic 35.0%
TCCATGGGAATGTCATTAAT+AGG + chr8.1:9571318-9571337 None:intergenic 35.0%
TCCTATTAATGACATTCCCA+TGG - chr8.1:9571314-9571333 MS.gene035236:CDS 35.0%
TCTATAATACCGCTGTTCAT+TGG - chr8.1:9571055-9571074 MS.gene035236:CDS 35.0%
! GGTGATATTATTGTAACGGA+CGG - chr8.1:9571369-9571388 MS.gene035236:CDS 35.0%
!! GTGATATTATTGTAACGGAC+GGG - chr8.1:9571370-9571389 MS.gene035236:CDS 35.0%
!! TGATATTATTGTAACGGACG+GGG - chr8.1:9571371-9571390 MS.gene035236:CDS 35.0%
!!! ACTTTTTGAGATGTCTCTTC+CGG - chr8.1:9571140-9571159 MS.gene035236:CDS 35.0%
!!! CCACTTTTTGTTGAGCATAA+AGG + chr8.1:9571351-9571370 None:intergenic 35.0%
AAAAGCCAACCAATGAACAG+CGG + chr8.1:9571067-9571086 None:intergenic 40.0%
AATACACAGTGCATTCGTCT+TGG - chr8.1:9571274-9571293 MS.gene035236:CDS 40.0%
ATTATAGAACAACCCTGCTC+TGG + chr8.1:9571043-9571062 None:intergenic 40.0%
TAATACCGCTGTTCATTGGT+TGG - chr8.1:9571059-9571078 MS.gene035236:CDS 40.0%
TCCACGTCCATAGTAATAGA+AGG + chr8.1:9571448-9571467 None:intergenic 40.0%
TCCTTCTATTACTATGGACG+TGG - chr8.1:9571444-9571463 MS.gene035236:CDS 40.0%
TTATAGAACAACCCTGCTCT+GGG + chr8.1:9571042-9571061 None:intergenic 40.0%
!! GATATTATTGTAACGGACGG+GGG - chr8.1:9571372-9571391 MS.gene035236:CDS 40.0%
ACTCTGATAAGCCCAGAGCA+GGG - chr8.1:9571028-9571047 MS.gene035236:CDS 50.0%
! TTAAGCTTGCTCACTGGCAC+CGG + chr8.1:9571511-9571530 None:intergenic 50.0%
GACTCTGATAAGCCCAGAGC+AGG - chr8.1:9571027-9571046 MS.gene035236:CDS 55.0%
GAGTCTCTGCTTTCACTCCC+CGG - chr8.1:9571489-9571508 MS.gene035236:CDS 55.0%
! TAAGCTTGCTCACTGGCACC+GGG + chr8.1:9571510-9571529 None:intergenic 55.0%
!! ATTGTAACGGACGGGGGTAC+TGG - chr8.1:9571378-9571397 MS.gene035236:CDS 55.0%
! AAGCTTGCTCACTGGCACCG+GGG + chr8.1:9571509-9571528 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 9570919 9571530 9570919 ID=MS.gene035236
chr8.1 mRNA 9570919 9571530 9570919 ID=MS.gene035236.t1;Parent=MS.gene035236
chr8.1 exon 9570919 9571530 9570919 ID=MS.gene035236.t1.exon1;Parent=MS.gene035236.t1
chr8.1 CDS 9570919 9571530 9570919 ID=cds.MS.gene035236.t1;Parent=MS.gene035236.t1
Gene Sequence

>MS.gene035236

ATGTTTGGTTATCGCCGATTAGCTGATATTTCATCACGTTTGTATGTTTTCTCATTGAGAGATAATAAATGGAGAAAAATTGAGGGTACTCACTTCCCTTATATGAAAGACTCTGATAAGCCCAGAGCAGGGTTGTTCTATAATACCGCTGTTCATTGGTTGGCTTTTAATGATGATTTAACATTGGTTGTTATTGTTGCCTTTGATTTAATGGAAATGAAACTTTTTGAGATGTCTCTTCCGGATGATTTTTACCATGATCCTACATATTGTGATTTATGGGTATTTGGAGTTTTTCTTAGTCTATGGGCTATTGATAAATATAATACTACAATTGAAATATGGGTGATGAATGAATACACAGTGCATTCGTCTTGGACTAAGTTTCTTGTTCTTCCTATTAATGACATTCCCATGGAATACTTTATCCCTTTATGCTCAACAAAAAGTGGTGATATTATTGTAACGGACGGGGGTACTGGATTGCTCAAGTATAATGATAAAGGACAACTGCTAGAGCATAACTCCTTCTATTACTATGGACGTGGATCCGAATTTGCTATGTATACAGAGTCTCTGCTTTCACTCCCCGGTGCCAGTGAGCAAGCTTAA

Protein sequence

>MS.gene035236.t1

MFGYRRLADISSRLYVFSLRDNKWRKIEGTHFPYMKDSDKPRAGLFYNTAVHWLAFNDDLTLVVIVAFDLMEMKLFEMSLPDDFYHDPTYCDLWVFGVFLSLWAIDKYNTTIEIWVMNEYTVHSSWTKFLVLPINDIPMEYFIPLCSTKSGDIIVTDGGTGLLKYNDKGQLLEHNSFYYYGRGSEFAMYTESLLSLPGASEQA