AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene019505


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene019505.t1 MTR_8g433030 72.222 180 41 5 2 177 163 337 2.09e-78 237
MS.gene019505.t1 MTR_8g032390 72.222 180 41 5 2 177 196 370 4.23e-78 237
MS.gene019505.t1 MTR_8g433040 68.478 184 41 3 2 182 195 364 4.96e-78 237
MS.gene019505.t1 MTR_8g032380 68.478 184 41 3 2 182 195 364 4.96e-78 237
MS.gene019505.t1 MTR_3g103020 55.263 190 69 6 2 182 196 378 7.65e-60 191
MS.gene019505.t1 MTR_6g060650 54.011 187 69 6 2 181 196 372 3.63e-57 184
MS.gene019505.t1 MTR_4g117720 55.385 195 63 9 2 182 194 378 6.49e-55 178
MS.gene019505.t1 MTR_4g109280 50.794 189 76 6 2 182 180 359 9.40e-53 172
MS.gene019505.t1 MTR_4g116330 51.323 189 77 5 2 182 199 380 1.64e-51 169
MS.gene019505.t1 MTR_4g122850 55.801 181 66 7 9 182 191 364 4.79e-51 168
MS.gene019505.t1 MTR_4g109270 54.491 167 64 5 23 182 203 364 5.00e-51 167
MS.gene019505.t1 MTR_6g463430 52.174 184 78 6 2 182 26 202 6.99e-51 162
MS.gene019505.t1 MTR_8g040935 48.969 194 79 6 1 182 23 208 1.01e-50 162
MS.gene019505.t1 MTR_4g094365 53.191 188 74 6 2 182 184 364 1.30e-50 167
MS.gene019505.t1 MTR_3g034180 49.231 195 74 8 2 182 306 489 5.31e-50 168
MS.gene019505.t1 MTR_4g116320 51.087 184 77 5 1 178 78 254 2.74e-49 160
MS.gene019505.t1 MTR_4g021785 50.538 186 81 6 1 182 177 355 6.05e-49 162
MS.gene019505.t1 MTR_4g023070 48.731 197 76 6 2 181 181 369 4.75e-48 160
MS.gene019505.t1 MTR_4g023130 49.730 185 79 6 2 181 137 312 6.11e-48 158
MS.gene019505.t1 MTR_4g023080 48.649 185 81 6 2 181 178 353 6.58e-47 157
MS.gene019505.t1 MTR_4g118380 53.571 168 54 6 2 155 198 355 1.02e-46 156
MS.gene019505.t1 MTR_4g117850 50.000 186 80 6 1 178 195 375 1.21e-46 157
MS.gene019505.t1 MTR_4g023120 47.475 198 79 6 2 182 181 370 3.15e-46 155
MS.gene019505.t1 MTR_1g055175 48.969 194 73 6 2 181 19 200 4.40e-46 150
MS.gene019505.t1 MTR_3g034160 50.521 192 74 6 2 181 192 374 1.20e-45 154
MS.gene019505.t1 MTR_4g023090 47.525 202 76 8 2 182 194 386 8.73e-45 152
MS.gene019505.t1 MTR_5g097210 55.901 161 54 5 27 179 209 360 2.03e-44 151
MS.gene019505.t1 MTR_4g023380 48.000 200 68 8 15 182 184 379 1.17e-43 149
MS.gene019505.t1 MTR_1g055135 49.479 192 82 6 1 182 189 375 2.05e-43 148
MS.gene019505.t1 MTR_3g034130 53.061 147 56 4 12 154 209 346 7.51e-43 147
MS.gene019505.t1 MTR_4g023200 47.872 188 80 7 2 180 180 358 1.28e-41 143
MS.gene019505.t1 MTR_1g055165 52.174 161 66 4 28 182 163 318 3.08e-41 141
MS.gene019505.t1 MTR_4g023190 47.872 188 79 7 2 180 180 357 6.72e-41 141
MS.gene019505.t1 MTR_1g055195 48.454 194 71 10 2 178 293 474 1.38e-39 140
MS.gene019505.t1 MTR_4g023150 47.872 188 80 7 2 180 180 358 1.46e-39 138
MS.gene019505.t1 MTR_1g055250 47.727 176 76 6 1 169 87 253 2.08e-38 134
MS.gene019505.t1 MTR_4g118400 48.087 183 66 8 1 176 134 294 7.89e-38 132
MS.gene019505.t1 MTR_4g118370 45.263 190 64 7 1 179 97 257 1.67e-37 130
MS.gene019505.t1 MTR_5g070130 46.667 195 85 9 2 182 188 377 2.18e-36 130
MS.gene019505.t1 MTR_7g079400 44.560 193 89 6 2 182 151 337 5.21e-36 128
MS.gene019505.t1 MTR_4g117780 45.789 190 88 7 2 182 184 367 6.53e-36 128
MS.gene019505.t1 MTR_5g070080 48.810 168 74 6 22 182 224 386 7.92e-36 129
MS.gene019505.t1 MTR_4g117770 46.486 185 80 7 13 182 187 367 1.73e-33 122
MS.gene019505.t1 MTR_4g117810 46.486 185 80 7 13 182 187 367 1.73e-33 122
MS.gene019505.t1 MTR_4g117870 51.538 130 55 3 58 182 141 267 2.34e-33 119
MS.gene019505.t1 MTR_8g102520 41.667 180 92 5 10 182 149 322 2.07e-32 118
MS.gene019505.t1 MTR_4g118360 51.163 129 51 4 61 181 1 125 2.11e-31 110
MS.gene019505.t1 MTR_4g117750 45.226 199 79 8 1 181 172 358 3.00e-31 118
MS.gene019505.t1 MTR_4g117750 54.386 114 38 4 76 180 373 481 1.45e-29 113
MS.gene019505.t1 MTR_7g079370 42.077 183 91 7 2 176 175 350 5.92e-31 115
MS.gene019505.t1 MTR_5g070088 46.626 163 73 7 29 182 229 386 6.09e-30 113
MS.gene019505.t1 MTR_7g079420 40.107 187 97 7 5 182 964 1144 2.43e-29 114
MS.gene019505.t1 MTR_4g117530 40.107 187 66 7 2 182 107 253 6.70e-29 107
MS.gene019505.t1 MTR_4g121580 46.939 147 47 6 4 149 200 316 1.76e-28 109
MS.gene019505.t1 MTR_5g023000 59.140 93 32 2 96 182 7 99 3.05e-27 99.0
MS.gene019505.t1 MTR_4g023440 38.919 185 64 4 2 182 193 332 3.35e-27 105
MS.gene019505.t1 MTR_7g079450 43.357 143 70 4 45 182 1 137 2.63e-26 97.8
MS.gene019505.t1 MTR_4g116330 38.095 189 63 5 2 182 199 341 2.87e-26 102
MS.gene019505.t1 MTR_4g122860 38.462 182 67 6 16 182 169 320 3.08e-26 102
MS.gene019505.t1 MTR_8g098830 41.611 149 70 6 10 151 54 192 1.38e-23 93.6
MS.gene019505.t1 MTR_2g048580 32.620 187 108 7 2 182 168 342 3.25e-19 83.6
MS.gene019505.t1 MTR_4g117430 50.588 85 34 5 2 85 75 152 4.33e-16 72.0
MS.gene019505.t1 MTR_8g013010 50.000 84 33 3 2 85 235 309 9.21e-16 73.9
MS.gene019505.t1 MTR_2g426020 29.762 168 101 6 25 178 218 382 3.42e-14 69.7
MS.gene019505.t1 MTR_4g023430 39.623 106 53 3 86 182 119 222 1.52e-13 66.6
MS.gene019505.t1 MTR_7g117720 51.724 87 30 4 101 176 186 271 2.70e-13 66.6
MS.gene019505.t1 MTR_4g114700 42.268 97 47 3 86 173 122 218 3.35e-13 65.9
MS.gene019505.t1 MTR_2g028110 28.729 181 111 7 8 181 197 366 4.43e-11 60.8
MS.gene019505.t1 MTR_5g057790 32.143 140 81 5 23 155 243 375 8.17e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene019505.t1 AT3G06240 27.647 170 117 2 9 174 252 419 4.09e-12 63.9

Find 46 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGTTGTCTTTGATTTAA+TGG 0.084690 8.1:-69773415 MS.gene019505:CDS
GGAATAGGAACTTTCATAAT+TGG 0.168463 8.1:-69773299 MS.gene019505:CDS
GATTATGGTTTGTGGGTATT+TGG 0.208731 8.1:-69773339 MS.gene019505:CDS
GGAAATCGACAGTTGAAATA+TGG 0.217300 8.1:-69773278 MS.gene019505:CDS
AAAGCAGGATTATTCTTTAA+TGG 0.228597 8.1:-69773489 MS.gene019505:CDS
AAGCAGGATTATTCTTTAAT+GGG 0.287791 8.1:-69773488 MS.gene019505:CDS
TTGGAGAATTTCTCAGTCTA+TGG 0.288501 8.1:-69773320 MS.gene019505:CDS
TAATCCTGCTTTGGAAGCTT+TGG 0.296435 8.1:+69773500 None:intergenic
GACCATGATCCTGTGGATTA+TGG 0.306412 8.1:-69773354 MS.gene019505:CDS
ATCTGAAGTGGCCATTTATA+CGG 0.348367 8.1:-69773070 MS.gene019505:CDS
AATCCTGCTTTGGAAGCTTT+GGG 0.358664 8.1:+69773501 None:intergenic
TCTTTAATGGGGATATTCAT+TGG 0.368049 8.1:-69773476 MS.gene019505:CDS
ATTAAAGAATAATCCTGCTT+TGG 0.391144 8.1:+69773491 None:intergenic
AAGCAGAGATTCCGTATAAA+TGG 0.404097 8.1:+69773059 None:intergenic
AATTTCTCAGTCTATGGAAT+AGG 0.433887 8.1:-69773314 MS.gene019505:CDS
GGATCATGGTCAAAATCATC+TGG 0.436812 8.1:+69773366 None:intergenic
CATCTTGTTAAACTTGTTCA+CGG 0.438416 8.1:+69773022 None:intergenic
TCCTGTGGATTATGGTTTGT+GGG 0.455259 8.1:-69773346 MS.gene019505:CDS
ATCCTGTGGATTATGGTTTG+TGG 0.458998 8.1:-69773347 MS.gene019505:CDS
GAAATCGACAGTTGAAATAT+GGG 0.469274 8.1:-69773277 MS.gene019505:CDS
TATAGGGGAAAAGAACATAA+TGG 0.470496 8.1:+69773200 None:intergenic
TATCCCAAAGCTTCCAAAGC+AGG 0.472312 8.1:-69773504 MS.gene019505:CDS
TAATGGGGATATTCATTGGT+TGG 0.487283 8.1:-69773472 MS.gene019505:CDS
ATGGCCACTTCAGATGCATT+CGG 0.499630 8.1:+69773078 None:intergenic
TAAACTTGTTCACGGTCACC+AGG 0.501824 8.1:+69773030 None:intergenic
AATACAAACAACATTCGTCT+TGG 0.505881 8.1:-69773245 MS.gene019505:CDS
AGTTGAAATATGGGTGATGA+AGG 0.512041 8.1:-69773268 MS.gene019505:CDS
GAATCTCTGCTTTCACTCCC+TGG 0.518981 8.1:-69773048 MS.gene019505:CDS
GGGGAAAAGAACATAATGGT+AGG 0.526109 8.1:+69773204 None:intergenic
TTGTCTTTGATTTAATGGAA+AGG 0.526787 8.1:-69773410 MS.gene019505:CDS
AACCATAATCCACAGGATCA+TGG 0.536939 8.1:+69773352 None:intergenic
AGCAGGATTATTCTTTAATG+GGG 0.562885 8.1:-69773487 MS.gene019505:CDS
TGGTCAAAATCATCTGGAAG+AGG 0.566442 8.1:+69773372 None:intergenic
AATATGTGGAATGAAATTGT+AGG 0.570086 8.1:-69773558 MS.gene019505:CDS
CATCACTTATTTGAATACCA+CGG 0.570605 8.1:-69773529 MS.gene019505:CDS
GGGAAAAGAACATAATGGTA+GGG 0.570690 8.1:+69773205 None:intergenic
AACTGACGGTGATCGTGGAT+TGG 0.582084 8.1:-69773148 MS.gene019505:CDS
GGAAGCTTTGGGATAATCCG+TGG 0.585102 8.1:+69773512 None:intergenic
ACCCACAAACCATAATCCAC+AGG 0.594022 8.1:+69773345 None:intergenic
GAATCCGAATGCATCTGAAG+TGG 0.598471 8.1:-69773082 MS.gene019505:CDS
GGTAGGGATTACAAGAGACT+TGG 0.600714 8.1:+69773221 None:intergenic
GTTGGAACTGACGGTGATCG+TGG 0.645956 8.1:-69773153 MS.gene019505:CDS
GGTGATATTGTTGGAACTGA+CGG 0.665403 8.1:-69773162 MS.gene019505:CDS
AAACTTGTTCACGGTCACCA+GGG 0.678032 8.1:+69773031 None:intergenic
TTATTATCATGATATACGAG+TGG 0.685713 8.1:-69773442 MS.gene019505:CDS
GTATTCAAATAAGTGATGTG+AGG 0.692775 8.1:+69773534 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTGTTGTCTTTGATTTAA+TGG - chr8.1:69773169-69773188 MS.gene019505:CDS 20.0%
! AAAGCAGGATTATTCTTTAA+TGG - chr8.1:69773095-69773114 MS.gene019505:CDS 25.0%
! AAGCAGGATTATTCTTTAAT+GGG - chr8.1:69773096-69773115 MS.gene019505:CDS 25.0%
! ATTAAAGAATAATCCTGCTT+TGG + chr8.1:69773096-69773115 None:intergenic 25.0%
! TTATTATCATGATATACGAG+TGG - chr8.1:69773142-69773161 MS.gene019505:CDS 25.0%
!! ACTTTTTGTAGAGTATATAG+GGG + chr8.1:69773402-69773421 None:intergenic 25.0%
!! TTGTCTTTGATTTAATGGAA+AGG - chr8.1:69773174-69773193 MS.gene019505:CDS 25.0%
!!! CACTTTTTGTAGAGTATATA+GGG + chr8.1:69773403-69773422 None:intergenic 25.0%
AATACAAACAACATTCGTCT+TGG - chr8.1:69773339-69773358 MS.gene019505:CDS 30.0%
AATTTCTCAGTCTATGGAAT+AGG - chr8.1:69773270-69773289 MS.gene019505:CDS 30.0%
ACAAAAAGTGGTGATATTGT+TGG - chr8.1:69773413-69773432 MS.gene019505:CDS 30.0%
AGCAGGATTATTCTTTAATG+GGG - chr8.1:69773097-69773116 MS.gene019505:CDS 30.0%
CATCACTTATTTGAATACCA+CGG - chr8.1:69773055-69773074 MS.gene019505:CDS 30.0%
CCTATATACTCTACAAAAAG+TGG - chr8.1:69773401-69773420 MS.gene019505:CDS 30.0%
GAAATCGACAGTTGAAATAT+GGG - chr8.1:69773307-69773326 MS.gene019505:CDS 30.0%
GGAATAGGAACTTTCATAAT+TGG - chr8.1:69773285-69773304 MS.gene019505:CDS 30.0%
GTATTCAAATAAGTGATGTG+AGG + chr8.1:69773053-69773072 None:intergenic 30.0%
TATAGGGGAAAAGAACATAA+TGG + chr8.1:69773387-69773406 None:intergenic 30.0%
TCTTTAATGGGGATATTCAT+TGG - chr8.1:69773108-69773127 MS.gene019505:CDS 30.0%
!!! CCACTTTTTGTAGAGTATAT+AGG + chr8.1:69773404-69773423 None:intergenic 30.0%
AAGCAGAGATTCCGTATAAA+TGG + chr8.1:69773528-69773547 None:intergenic 35.0%
AGTTGAAATATGGGTGATGA+AGG - chr8.1:69773316-69773335 MS.gene019505:CDS 35.0%
GGAAATCGACAGTTGAAATA+TGG - chr8.1:69773306-69773325 MS.gene019505:CDS 35.0%
GGGAAAAGAACATAATGGTA+GGG + chr8.1:69773382-69773401 None:intergenic 35.0%
TAATGGGGATATTCATTGGT+TGG - chr8.1:69773112-69773131 MS.gene019505:CDS 35.0%
TTGGAGAATTTCTCAGTCTA+TGG - chr8.1:69773264-69773283 MS.gene019505:CDS 35.0%
! ATCTGAAGTGGCCATTTATA+CGG - chr8.1:69773514-69773533 MS.gene019505:CDS 35.0%
! GATTATGGTTTGTGGGTATT+TGG - chr8.1:69773245-69773264 MS.gene019505:CDS 35.0%
AACCATAATCCACAGGATCA+TGG + chr8.1:69773235-69773254 None:intergenic 40.0%
GGATCATGGTCAAAATCATC+TGG + chr8.1:69773221-69773240 None:intergenic 40.0%
GGGGAAAAGAACATAATGGT+AGG + chr8.1:69773383-69773402 None:intergenic 40.0%
GGTGATATTGTTGGAACTGA+CGG - chr8.1:69773422-69773441 MS.gene019505:CDS 40.0%
TGGTCAAAATCATCTGGAAG+AGG + chr8.1:69773215-69773234 None:intergenic 40.0%
! ATCCTGTGGATTATGGTTTG+TGG - chr8.1:69773237-69773256 MS.gene019505:CDS 40.0%
! TCCTGTGGATTATGGTTTGT+GGG - chr8.1:69773238-69773257 MS.gene019505:CDS 40.0%
!! AATCCTGCTTTGGAAGCTTT+GGG + chr8.1:69773086-69773105 None:intergenic 40.0%
!! TAATCCTGCTTTGGAAGCTT+TGG + chr8.1:69773087-69773106 None:intergenic 40.0%
!! TGATTTTGACCATGATCCTG+TGG - chr8.1:69773223-69773242 MS.gene019505:CDS 40.0%
ACCCACAAACCATAATCCAC+AGG + chr8.1:69773242-69773261 None:intergenic 45.0%
ATGGCCACTTCAGATGCATT+CGG + chr8.1:69773509-69773528 None:intergenic 45.0%
GAATCCGAATGCATCTGAAG+TGG - chr8.1:69773502-69773521 MS.gene019505:CDS 45.0%
GGTAGGGATTACAAGAGACT+TGG + chr8.1:69773366-69773385 None:intergenic 45.0%
TATCCCAAAGCTTCCAAAGC+AGG - chr8.1:69773080-69773099 MS.gene019505:CDS 45.0%
! AAACTTGTTCACGGTCACCA+GGG + chr8.1:69773556-69773575 None:intergenic 45.0%
! GACCATGATCCTGTGGATTA+TGG - chr8.1:69773230-69773249 MS.gene019505:CDS 45.0%
! TAAACTTGTTCACGGTCACC+AGG + chr8.1:69773557-69773576 None:intergenic 45.0%
AACTGACGGTGATCGTGGAT+TGG - chr8.1:69773436-69773455 MS.gene019505:CDS 50.0%
GAATCTCTGCTTTCACTCCC+TGG - chr8.1:69773536-69773555 MS.gene019505:CDS 50.0%
!! GGAAGCTTTGGGATAATCCG+TGG + chr8.1:69773075-69773094 None:intergenic 50.0%
GTTGGAACTGACGGTGATCG+TGG - chr8.1:69773431-69773450 MS.gene019505:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 69773029 69773577 69773029 ID=MS.gene019505
chr8.1 mRNA 69773029 69773577 69773029 ID=MS.gene019505.t1;Parent=MS.gene019505
chr8.1 exon 69773029 69773577 69773029 ID=MS.gene019505.t1.exon1;Parent=MS.gene019505.t1
chr8.1 CDS 69773029 69773577 69773029 ID=cds.MS.gene019505.t1;Parent=MS.gene019505.t1
Gene Sequence

>MS.gene019505

ATGTGGAATGAAATTGTAGGTCCTCACATCACTTATTTGAATACCACGGATTATCCCAAAGCTTCCAAAGCAGGATTATTCTTTAATGGGGATATTCATTGGTTGGCATCTCGTTATTATCATGATATACGAGTGGAAGTTATTGTTGTCTTTGATTTAATGGAAAGGAAACTGTTAGAGATACCTCTTCCAGATGATTTTGACCATGATCCTGTGGATTATGGTTTGTGGGTATTTGGAGAATTTCTCAGTCTATGGAATAGGAACTTTCATAATTGGAAATCGACAGTTGAAATATGGGTGATGAAGGAATACAAACAACATTCGTCTTGGACCAAGTCTCTTGTAATCCCTACCATTATGTTCTTTTCCCCTATATACTCTACAAAAAGTGGTGATATTGTTGGAACTGACGGTGATCGTGGATTGGTGAAGTATAATAATAAACGAAAGTCGAAAGAGTATCTCGAAATGAATCCGAATGCATCTGAAGTGGCCATTTATACGGAATCTCTGCTTTCACTCCCTGGTGACCGTGAACAAGTTTAA

Protein sequence

>MS.gene019505.t1

MWNEIVGPHITYLNTTDYPKASKAGLFFNGDIHWLASRYYHDIRVEVIVVFDLMERKLLEIPLPDDFDHDPVDYGLWVFGEFLSLWNRNFHNWKSTVEIWVMKEYKQHSSWTKSLVIPTIMFFSPIYSTKSGDIVGTDGDRGLVKYNNKRKSKEYLEMNPNASEVAIYTESLLSLPGDREQV