AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57537

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57537.t1 MTR_8g032390 57.258 372 95 6 37 346 1 370 1.34e-127 370
MS.gene57537.t1 MTR_8g433040 66.795 259 68 3 95 346 112 359 5.19e-112 330
MS.gene57537.t1 MTR_8g032380 66.795 259 68 3 95 346 112 359 5.19e-112 330
MS.gene57537.t1 MTR_8g433030 57.227 339 114 8 37 346 1 337 7.56e-110 324
MS.gene57537.t1 MTR_4g023130 48.562 313 112 9 70 346 9 308 2.53e-82 253
MS.gene57537.t1 MTR_6g060650 53.817 262 105 6 94 345 112 367 6.34e-82 254
MS.gene57537.t1 MTR_4g117720 46.784 342 123 10 56 346 39 372 1.24e-80 251
MS.gene57537.t1 MTR_4g094365 52.030 271 118 6 83 345 92 358 4.93e-79 246
MS.gene57537.t1 MTR_4g122850 55.263 266 100 11 95 346 99 359 9.07e-78 243
MS.gene57537.t1 MTR_4g118380 52.344 256 99 6 86 324 106 355 4.66e-76 238
MS.gene57537.t1 MTR_3g103020 51.908 262 112 6 95 346 116 373 5.00e-76 239
MS.gene57537.t1 MTR_4g021785 51.172 256 111 6 95 346 105 350 6.59e-75 235
MS.gene57537.t1 MTR_4g023080 51.163 258 107 8 95 346 105 349 4.58e-72 228
MS.gene57537.t1 MTR_4g023090 48.352 273 116 6 95 347 113 380 9.24e-72 228
MS.gene57537.t1 MTR_4g116330 50.000 262 113 7 95 346 122 375 2.09e-70 224
MS.gene57537.t1 MTR_4g023380 44.554 303 121 7 71 347 92 373 3.15e-69 221
MS.gene57537.t1 MTR_4g023070 47.405 289 118 9 82 346 87 365 8.51e-69 220
MS.gene57537.t1 MTR_4g117850 49.624 266 111 7 94 344 116 373 1.25e-68 220
MS.gene57537.t1 MTR_3g034180 45.000 320 139 12 48 346 181 484 5.89e-68 221
MS.gene57537.t1 MTR_4g109270 49.624 266 109 9 95 346 105 359 6.59e-66 213
MS.gene57537.t1 MTR_4g023120 44.983 289 125 8 82 346 87 365 8.38e-66 213
MS.gene57537.t1 MTR_3g034160 45.110 317 143 9 48 346 67 370 1.95e-65 211
MS.gene57537.t1 MTR_1g055135 44.518 301 131 9 62 346 90 370 2.71e-64 209
MS.gene57537.t1 MTR_4g023190 47.653 277 117 10 82 346 94 354 4.32e-64 207
MS.gene57537.t1 MTR_4g117780 46.617 266 129 7 90 346 101 362 1.14e-63 207
MS.gene57537.t1 MTR_3g034130 49.600 250 95 9 95 323 107 346 1.16e-62 204
MS.gene57537.t1 MTR_4g109280 49.049 263 111 8 95 344 100 352 6.03e-62 202
MS.gene57537.t1 MTR_4g023150 47.292 277 119 11 82 346 94 355 7.05e-62 202
MS.gene57537.t1 MTR_4g117770 46.350 274 133 8 82 346 94 362 1.48e-60 199
MS.gene57537.t1 MTR_4g117810 46.350 274 133 8 82 346 94 362 1.48e-60 199
MS.gene57537.t1 MTR_4g023200 44.765 277 126 10 82 346 94 355 2.38e-60 198
MS.gene57537.t1 MTR_5g097210 45.956 272 123 7 95 353 105 365 9.68e-60 197
MS.gene57537.t1 MTR_4g117870 43.321 277 101 8 84 346 28 262 2.09e-59 192
MS.gene57537.t1 MTR_5g070130 45.725 269 122 9 95 345 108 370 4.06e-58 193
MS.gene57537.t1 MTR_4g116320 44.097 288 105 6 70 345 9 252 1.22e-57 188
MS.gene57537.t1 MTR_1g055195 42.765 311 142 13 52 345 181 472 1.42e-57 194
MS.gene57537.t1 MTR_4g121580 46.457 254 100 8 95 340 113 338 7.31e-57 190
MS.gene57537.t1 MTR_4g118400 44.118 272 105 8 85 345 59 294 9.98e-57 187
MS.gene57537.t1 MTR_5g070080 43.820 267 124 9 95 346 125 380 2.55e-56 188
MS.gene57537.t1 MTR_7g079400 41.905 315 157 10 49 346 27 332 1.06e-54 182
MS.gene57537.t1 MTR_7g079370 44.649 271 127 10 95 345 83 350 1.41e-54 183
MS.gene57537.t1 MTR_4g117530 41.343 283 103 8 70 346 23 248 1.06e-51 172
MS.gene57537.t1 MTR_6g463430 52.660 188 80 6 163 346 15 197 4.86e-51 169
MS.gene57537.t1 MTR_5g070088 42.238 277 133 9 86 346 115 380 3.00e-50 172
MS.gene57537.t1 MTR_8g040935 48.500 200 92 4 156 346 6 203 2.48e-49 164
MS.gene57537.t1 MTR_4g023440 43.798 258 93 7 95 346 116 327 2.97e-49 168
MS.gene57537.t1 MTR_4g116330 42.366 262 94 7 95 346 122 336 3.23e-48 166
MS.gene57537.t1 MTR_4g117750 40.000 315 160 11 49 345 50 353 1.47e-47 167
MS.gene57537.t1 MTR_4g117750 53.774 106 43 2 247 346 373 478 8.54e-25 105
MS.gene57537.t1 MTR_8g102520 35.962 317 156 10 69 345 7 316 3.89e-46 160
MS.gene57537.t1 MTR_4g118370 41.767 249 108 7 109 346 33 255 1.28e-43 151
MS.gene57537.t1 MTR_1g055175 46.392 194 85 6 164 345 9 195 1.29e-42 147
MS.gene57537.t1 MTR_1g055165 34.062 320 139 9 34 346 59 313 7.70e-42 148
MS.gene57537.t1 MTR_4g117430 51.250 160 68 5 97 256 3 152 3.39e-40 139
MS.gene57537.t1 MTR_1g055250 37.943 282 113 10 70 338 21 253 3.93e-38 139
MS.gene57537.t1 MTR_8g013010 48.193 166 74 4 95 256 152 309 3.59e-35 130
MS.gene57537.t1 MTR_7g079420 37.879 264 120 10 96 345 905 1138 1.88e-34 134
MS.gene57537.t1 MTR_4g116340 51.799 139 56 4 91 222 113 247 1.20e-33 125
MS.gene57537.t1 MTR_4g114700 34.884 258 104 6 97 340 9 216 8.45e-32 120
MS.gene57537.t1 MTR_8g098830 41.176 187 89 8 154 329 25 201 8.90e-29 111
MS.gene57537.t1 MTR_2g048580 34.815 270 123 13 95 346 103 337 9.44e-29 114
MS.gene57537.t1 MTR_4g122860 31.834 289 140 10 70 345 70 314 1.73e-26 108
MS.gene57537.t1 MTR_7g117720 50.000 106 48 1 90 190 85 190 2.83e-25 103
MS.gene57537.t1 MTR_7g117720 45.977 87 37 3 270 346 186 272 2.88e-11 63.5
MS.gene57537.t1 MTR_5g023000 60.638 94 29 4 260 346 2 94 1.63e-24 96.3
MS.gene57537.t1 MTR_4g118360 53.333 105 41 3 247 345 18 120 1.70e-24 97.1
MS.gene57537.t1 MTR_7g079450 45.926 135 64 4 216 345 1 131 1.45e-22 92.4
MS.gene57537.t1 MTR_6g034720 47.748 111 48 3 139 243 73 179 7.16e-21 90.1
MS.gene57537.t1 MTR_3g083660 31.532 222 103 9 70 282 25 206 1.20e-16 79.3
MS.gene57537.t1 MTR_8g054320 28.956 297 167 13 70 348 32 302 6.89e-16 77.8
MS.gene57537.t1 MTR_2g021155 29.075 227 132 8 70 282 671 882 8.81e-16 79.3
MS.gene57537.t1 MTR_2g078870 28.205 195 117 6 93 282 56 232 1.24e-15 77.0
MS.gene57537.t1 MTR_4g133170 29.114 237 129 11 86 299 82 302 1.46e-15 77.4
MS.gene57537.t1 MTR_2g028130 24.545 330 197 13 60 345 70 391 1.32e-14 74.7
MS.gene57537.t1 MTR_4g055060 31.481 216 112 10 93 289 126 324 1.34e-14 74.7
MS.gene57537.t1 MTR_3g030320 27.225 191 127 5 93 282 44 223 1.85e-14 73.6
MS.gene57537.t1 MTR_7g009265 29.224 219 133 7 91 289 30 246 2.41e-14 73.6
MS.gene57537.t1 MTR_6g090180 27.692 260 150 8 97 325 161 413 3.38e-14 73.6
MS.gene57537.t1 MTR_2g426020 26.644 289 169 13 93 345 99 380 3.53e-14 73.6
MS.gene57537.t1 MTR_1g068835 27.941 204 127 8 93 282 90 287 5.78e-14 72.8
MS.gene57537.t1 MTR_0667s0010 25.497 302 162 13 93 345 93 380 6.15e-14 72.8
MS.gene57537.t1 MTR_1g097560 27.341 267 161 12 90 343 127 373 8.78e-14 72.0
MS.gene57537.t1 MTR_5g007610 31.250 224 119 11 88 287 125 337 9.32e-14 72.4
MS.gene57537.t1 MTR_3g009310 32.258 186 104 8 110 289 160 329 1.11e-13 72.0
MS.gene57537.t1 MTR_6g017295 26.606 218 135 7 91 287 40 253 1.15e-13 72.4
MS.gene57537.t1 MTR_3g013740 31.905 210 112 9 93 286 148 342 4.30e-13 70.1
MS.gene57537.t1 MTR_5g076490 25.207 242 149 6 133 346 97 334 4.69e-13 69.7
MS.gene57537.t1 MTR_3g036430 22.500 280 140 9 60 282 10 269 1.11e-12 68.6
MS.gene57537.t1 MTR_7g445890 25.503 298 133 11 70 287 16 304 1.12e-12 68.9
MS.gene57537.t1 MTR_0129s0050 25.795 283 163 7 106 346 129 406 1.38e-12 68.6
MS.gene57537.t1 MTR_3g009260 29.000 200 124 8 89 282 127 314 1.46e-12 68.6
MS.gene57537.t1 MTR_3g083010 25.263 285 169 14 98 347 122 397 1.77e-12 68.6
MS.gene57537.t1 MTR_2g021800 24.920 313 192 10 60 340 107 408 1.98e-12 68.2
MS.gene57537.t1 MTR_2g025420 25.333 300 169 16 90 343 102 392 2.01e-12 68.2
MS.gene57537.t1 MTR_5g057710 30.000 210 111 10 93 286 141 330 2.41e-12 67.8
MS.gene57537.t1 MTR_3g007330 30.097 206 116 7 95 287 138 328 2.49e-12 67.8
MS.gene57537.t1 MTR_6g065510 27.899 276 148 13 43 287 2 257 3.71e-12 67.0
MS.gene57537.t1 MTR_7g062100 31.220 205 117 9 95 282 122 319 3.93e-12 67.4
MS.gene57537.t1 MTR_5g057790 29.952 207 115 10 93 286 141 330 4.47e-12 67.0
MS.gene57537.t1 MTR_2g024100 26.087 276 162 12 105 345 117 385 4.60e-12 67.0
MS.gene57537.t1 MTR_3g436150 27.014 211 133 7 90 287 130 332 5.11e-12 67.0
MS.gene57537.t1 MTR_3g036290 29.104 268 151 14 93 348 118 358 5.71e-12 66.6
MS.gene57537.t1 MTR_1g068860 26.190 210 133 8 89 282 86 289 6.64e-12 66.6
MS.gene57537.t1 MTR_3g083640 27.273 198 112 11 94 282 156 330 7.25e-12 66.6
MS.gene57537.t1 MTR_2g104340 28.369 282 165 13 98 346 117 394 8.15e-12 66.2
MS.gene57537.t1 MTR_1g055200 46.667 75 33 3 95 168 7 75 9.05e-12 61.6
MS.gene57537.t1 MTR_3g024110 28.205 195 114 7 93 282 200 373 1.00e-11 66.2
MS.gene57537.t1 MTR_2g426060 24.342 304 178 14 83 345 88 380 1.08e-11 65.9
MS.gene57537.t1 MTR_3g014540 28.708 209 125 6 90 286 119 315 1.18e-11 65.9
MS.gene57537.t1 MTR_3g011060 31.308 214 111 9 95 290 133 328 1.80e-11 65.1
MS.gene57537.t1 MTR_2g031720 26.761 284 164 13 95 346 97 368 2.17e-11 65.1
MS.gene57537.t1 MTR_1004s0010 29.381 194 115 8 92 283 116 289 2.64e-11 64.7
MS.gene57537.t1 MTR_3g023930 24.609 256 156 9 93 347 128 347 3.13e-11 64.3
MS.gene57537.t1 MTR_0760s0010 29.381 194 115 8 92 283 146 319 3.44e-11 64.3
MS.gene57537.t1 MTR_2g031680 24.104 307 195 12 74 353 71 366 3.66e-11 64.3
MS.gene57537.t1 MTR_5g011200 27.830 212 137 8 91 296 114 315 3.93e-11 63.9
MS.gene57537.t1 MTR_5g046300 26.733 202 126 8 93 287 98 284 5.08e-11 63.5
MS.gene57537.t1 MTR_5g046290 26.733 202 126 8 93 287 98 284 5.08e-11 63.5
MS.gene57537.t1 MTR_3g030240 25.217 230 132 8 109 337 157 347 6.37e-11 63.5
MS.gene57537.t1 MTR_5g046280 26.733 202 126 8 93 287 1 187 6.48e-11 62.4
MS.gene57537.t1 MTR_2g028110 27.092 251 159 11 112 345 118 361 8.17e-11 63.2
MS.gene57537.t1 MTR_7g055830 26.568 271 138 10 73 289 16 279 8.63e-11 63.2
MS.gene57537.t1 MTR_2g426090 25.991 227 132 10 83 282 89 306 8.69e-11 63.2
MS.gene57537.t1 MTR_2g078900 28.736 174 106 6 110 282 153 309 8.96e-11 62.8
MS.gene57537.t1 MTR_3g030350 27.461 193 127 6 93 282 130 312 9.55e-11 63.2
MS.gene57537.t1 MTR_2g104280 26.644 289 173 13 94 346 118 403 9.70e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57537.t1 AT3G23880 26.471 204 137 6 90 289 110 304 2.03e-11 65.1

Find 80 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGTTGTCTTTGATTTAA+TGG 0.084690 8.3:-62187007 MS.gene57537:CDS
TATTATGATTTGTGGGTATT+TGG 0.191816 8.3:-62186931 MS.gene57537:CDS
ATAATTGGACAATTGAAATA+TGG 0.213992 8.3:-62186876 MS.gene57537:CDS
TTGGAAAATTTCTCAGTCTA+TGG 0.237424 8.3:-62186912 MS.gene57537:CDS
AAACGTAAAAGATACTTTAC+TGG 0.283571 8.3:+62187624 None:intergenic
CGACGTCAACCATATGGTTT+TGG 0.296265 8.3:-62187258 MS.gene57537:CDS
TAATCCTGCTTTGGAAGCTT+TGG 0.330098 8.3:+62187089 None:intergenic
GGTATTCAAATAAGTGAATT+GGG 0.335829 8.3:+62187122 None:intergenic
TGGTATTCAAATAAGTGAAT+TGG 0.351160 8.3:+62187121 None:intergenic
CACTAAAGCACCTGGATGTA+TGG 0.357662 8.3:+62187835 None:intergenic
TCTTCAATGGGGCTATTCAT+TGG 0.385937 8.3:-62187065 MS.gene57537:CDS
GAATCTGAATGCTTCTGAAT+TGG 0.391506 8.3:-62186680 MS.gene57537:CDS
ATTGAAGAATAATCCTGCTT+TGG 0.397965 8.3:+62187080 None:intergenic
AAAGCAGGATTATTCTTCAA+TGG 0.398001 8.3:-62187078 MS.gene57537:CDS
TAATTGGACAATTGAAATAT+GGG 0.403350 8.3:-62186875 MS.gene57537:CDS
TTGGTGAAGTTTAATGATAA+AGG 0.404506 8.3:-62186727 MS.gene57537:CDS
GGTGGCATGGCACATAAAAC+AGG 0.405514 8.3:-62187867 MS.gene57537:CDS
ATCTTGGGTATTATGATTTG+TGG 0.405974 8.3:-62186939 MS.gene57537:CDS
CAATGGGGCTATTCATTGGT+TGG 0.407106 8.3:-62187061 MS.gene57537:CDS
AAGCAGGATTATTCTTCAAT+GGG 0.407987 8.3:-62187077 MS.gene57537:CDS
GATTCTGTTGCGAGTAACCC+TGG 0.417016 8.3:+62187738 None:intergenic
CTTTAGTGAAAATCAGCTTC+GGG 0.420529 8.3:-62187820 MS.gene57537:CDS
TTCCTATATATTTCATCACT+TGG 0.421041 8.3:-62187767 MS.gene57537:CDS
GTTAAACTTGCACACGGTCC+AGG 0.424041 8.3:+62186628 None:intergenic
TCGATGATATGCAAGTGATT+TGG 0.442530 8.3:+62187278 None:intergenic
TCTCATTGAGAGATAATAAG+TGG 0.452326 8.3:-62187161 MS.gene57537:CDS
CCAAGTAATCATCTCTTGAT+TGG 0.454916 8.3:+62187229 None:intergenic
TCTTTGTGAAATCTAGAAGA+TGG 0.459093 8.3:+62187324 None:intergenic
AGATTGTGGTCAAAATCAAC+TGG 0.461270 8.3:+62186958 None:intergenic
TTCCAAGTGATGAAATATAT+AGG 0.463956 8.3:+62187765 None:intergenic
GAGTCTCCGCTTTCACTCCC+TGG 0.464258 8.3:-62186646 MS.gene57537:CDS
ATTCTGTTGCGAGTAACCCT+GGG 0.465001 8.3:+62187739 None:intergenic
GCTTTAGTGAAAATCAGCTT+CGG 0.465452 8.3:-62187821 MS.gene57537:CDS
TCTTGGGTATTATGATTTGT+GGG 0.466968 8.3:-62186938 MS.gene57537:CDS
ACTTTACTGGCAACCTCAGC+AGG 0.467390 8.3:+62187637 None:intergenic
AATTGAAATATGGGTGATGA+AGG 0.467515 8.3:-62186866 MS.gene57537:CDS
TATGCCAAAGCTTCCAAAGC+AGG 0.478827 8.3:-62187093 MS.gene57537:CDS
TATCATCGACGTCAACCATA+TGG 0.496619 8.3:-62187264 MS.gene57537:CDS
CCAATCAAGAGATGATTACT+TGG 0.499996 8.3:-62187229 MS.gene57537:CDS
AATACAAACAACATTCGTCT+TGG 0.503398 8.3:-62186843 MS.gene57537:CDS
GGAAGCTTTGGCATAATCCA+TGG 0.505733 8.3:+62187101 None:intergenic
AGCGAGAACTTCATCACGGT+TGG 0.511457 8.3:-62187190 MS.gene57537:CDS
GGCCCACTTGCCATACATCC+AGG 0.512532 8.3:-62187845 MS.gene57537:CDS
TGGTCATAACCAAAACCATA+TGG 0.514260 8.3:+62187249 None:intergenic
AACTGATGGTTGGAGTGGAT+TGG 0.514369 8.3:-62186746 MS.gene57537:CDS
AAATTAAAGGTTCATGCAGA+GGG 0.521334 8.3:-62187389 MS.gene57537:intron
TTGTCTTTGATTTAATGGAA+AGG 0.526787 8.3:-62187002 MS.gene57537:CDS
TGGTCAAAATCAACTGGAAG+AGG 0.526874 8.3:+62186964 None:intergenic
AATCAGCTTCGGGATAGCTT+CGG 0.527191 8.3:-62187810 MS.gene57537:CDS
GTTCAACAAACCTCTACGTA+TGG 0.527840 8.3:-62187350 MS.gene57537:CDS
ATCAAGAGATGATTACTTGG+TGG 0.531322 8.3:-62187226 MS.gene57537:CDS
ATTCACTTATTTGAATACCA+TGG 0.544429 8.3:-62187118 MS.gene57537:CDS
ATATAAGGGAAGAGCACAGA+AGG 0.545080 8.3:+62186797 None:intergenic
TCCGGAAAACATGGGTGGCA+TGG 0.546936 8.3:-62187880 None:intergenic
ATGATATGCAAGTGATTTGG+CGG 0.547475 8.3:+62187281 None:intergenic
GGTGATATTGTTGGAACTGA+TGG 0.549980 8.3:-62186760 MS.gene57537:CDS
TTAAACTTGCACACGGTCCA+GGG 0.552307 8.3:+62186629 None:intergenic
CACGGTCCAGGGAGTGAAAG+CGG 0.561772 8.3:+62186640 None:intergenic
GAAATTAAAGGTTCATGCAG+AGG 0.568478 8.3:-62187390 MS.gene57537:intron
GTTGGAACTGATGGTTGGAG+TGG 0.575710 8.3:-62186751 MS.gene57537:CDS
ATATTGTTGGAACTGATGGT+TGG 0.578132 8.3:-62186756 MS.gene57537:CDS
CAGAATCAAGAACGTGCAAG+AGG 0.579980 8.3:-62187722 MS.gene57537:CDS
ATTTGAATGAGTAATTCAGC+AGG 0.581559 8.3:+62187660 None:intergenic
TACTCATTCAAATCCTGCTG+AGG 0.585371 8.3:-62187650 MS.gene57537:CDS
AATCATAATACCCAAGATTG+TGG 0.590736 8.3:+62186944 None:intergenic
CCGTGATGAAGTTCTCGCTA+AGG 0.599812 8.3:+62187194 None:intergenic
GCACCTGGATGTATGGCAAG+TGG 0.602010 8.3:+62187842 None:intergenic
CACCTGGATGTATGGCAAGT+GGG 0.604062 8.3:+62187843 None:intergenic
TTCTGTTGCGAGTAACCCTG+GGG 0.626595 8.3:+62187740 None:intergenic
GTGCAAGAGGAGAATAAACA+TGG 0.633990 8.3:-62187709 MS.gene57537:CDS
CATCTTGTTAAACTTGCACA+CGG 0.639243 8.3:+62186622 None:intergenic
CCTTAGCGAGAACTTCATCA+CGG 0.641225 8.3:-62187194 MS.gene57537:CDS
GTATTCAAATAAGTGAATTG+GGG 0.643134 8.3:+62187123 None:intergenic
AAGCGGAGACTCTGTATACA+CGG 0.652046 8.3:+62186657 None:intergenic
AAGATGGATTCCATACGTAG+AGG 0.661639 8.3:+62187340 None:intergenic
AGCAGGATTATTCTTCAATG+GGG 0.673360 8.3:-62187076 MS.gene57537:CDS
TTGTTTCCGGAAAACATGGG+TGG 0.694578 8.3:-62187885 None:intergenic
TTGGCGGCAAAAGAAGACAA+AGG 0.698191 8.3:+62187297 None:intergenic
GTGGCATGGCACATAAAACA+GGG 0.708369 8.3:-62187866 MS.gene57537:CDS
TCTGTTGCGAGTAACCCTGG+GGG 0.755892 8.3:+62187741 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTCTTCTTATGAAATTAA+AGG - chr8.3:62187089-62187108 MS.gene57537:CDS 15.0%
!! ATAATTGGACAATTGAAATA+TGG - chr8.3:62187615-62187634 MS.gene57537:CDS 20.0%
!! TAATTGGACAATTGAAATAT+GGG - chr8.3:62187616-62187635 MS.gene57537:CDS 20.0%
!!! ACTTTTTGTAGAGTATATAA+GGG + chr8.3:62187711-62187730 None:intergenic 20.0%
!!! GGAAAAGAAATTTTCATAAT+TGG - chr8.3:62187600-62187619 MS.gene57537:intron 20.0%
!!! TATTGTTGTCTTTGATTTAA+TGG - chr8.3:62187484-62187503 MS.gene57537:intron 20.0%
! AAACGTAAAAGATACTTTAC+TGG + chr8.3:62186870-62186889 None:intergenic 25.0%
! ATTCACTTATTTGAATACCA+TGG - chr8.3:62187373-62187392 MS.gene57537:CDS 25.0%
! CTTATATACTCTACAAAAAG+TGG - chr8.3:62187710-62187729 MS.gene57537:CDS 25.0%
! GGTATTCAAATAAGTGAATT+GGG + chr8.3:62187372-62187391 None:intergenic 25.0%
! GTATTCAAATAAGTGAATTG+GGG + chr8.3:62187371-62187390 None:intergenic 25.0%
! TGGTATTCAAATAAGTGAAT+TGG + chr8.3:62187373-62187392 None:intergenic 25.0%
! TTCCAAGTGATGAAATATAT+AGG + chr8.3:62186729-62186748 None:intergenic 25.0%
! TTCCTATATATTTCATCACT+TGG - chr8.3:62186724-62186743 MS.gene57537:CDS 25.0%
! TTGGTGAAGTTTAATGATAA+AGG - chr8.3:62187764-62187783 MS.gene57537:CDS 25.0%
!! TATTATGATTTGTGGGTATT+TGG - chr8.3:62187560-62187579 MS.gene57537:intron 25.0%
!! TTGTCTTTGATTTAATGGAA+AGG - chr8.3:62187489-62187508 MS.gene57537:intron 25.0%
!!! CACTTTTTGTAGAGTATATA+AGG + chr8.3:62187712-62187731 None:intergenic 25.0%
AAAGCAGGATTATTCTTCAA+TGG - chr8.3:62187413-62187432 MS.gene57537:intron 30.0%
AAATTAAAGGTTCATGCAGA+GGG - chr8.3:62187102-62187121 MS.gene57537:CDS 30.0%
AAGCAGGATTATTCTTCAAT+GGG - chr8.3:62187414-62187433 MS.gene57537:intron 30.0%
AATACAAACAACATTCGTCT+TGG - chr8.3:62187648-62187667 MS.gene57537:CDS 30.0%
AATCATAATACCCAAGATTG+TGG + chr8.3:62187550-62187569 None:intergenic 30.0%
AATTGAAATATGGGTGATGA+AGG - chr8.3:62187625-62187644 MS.gene57537:CDS 30.0%
ACAAAAAGTGGTGATATTGT+TGG - chr8.3:62187722-62187741 MS.gene57537:CDS 30.0%
ATCTTGGGTATTATGATTTG+TGG - chr8.3:62187552-62187571 MS.gene57537:intron 30.0%
ATTGAAGAATAATCCTGCTT+TGG + chr8.3:62187414-62187433 None:intergenic 30.0%
ATTTGAATGAGTAATTCAGC+AGG + chr8.3:62186834-62186853 None:intergenic 30.0%
TATTAACACAATTCTTTCCG+CGG + chr8.3:62186967-62186986 None:intergenic 30.0%
TCTCATTGAGAGATAATAAG+TGG - chr8.3:62187330-62187349 MS.gene57537:CDS 30.0%
TCTTTGTGAAATCTAGAAGA+TGG + chr8.3:62187170-62187189 None:intergenic 30.0%
TGAAACAGAATCATGGTTAA+TGG + chr8.3:62187039-62187058 None:intergenic 30.0%
TTCATAAGAAGAATAACGTC+AGG + chr8.3:62187084-62187103 None:intergenic 30.0%
TTGGAAAATTTCTCAGTCTA+TGG - chr8.3:62187579-62187598 MS.gene57537:intron 30.0%
! ATGCAGAGGGTTTATATTTT+TGG - chr8.3:62187115-62187134 MS.gene57537:CDS 30.0%
! TCTTGGGTATTATGATTTGT+GGG - chr8.3:62187553-62187572 MS.gene57537:intron 30.0%
!! TTGATTTTGACCACAATCTT+GGG - chr8.3:62187537-62187556 MS.gene57537:intron 30.0%
AGATTGTGGTCAAAATCAAC+TGG + chr8.3:62187536-62187555 None:intergenic 35.0%
AGCAGGATTATTCTTCAATG+GGG - chr8.3:62187415-62187434 MS.gene57537:intron 35.0%
ATATAGGAATTCGCAAAGTG+AGG + chr8.3:62186936-62186955 None:intergenic 35.0%
ATATTGTTGGAACTGATGGT+TGG - chr8.3:62187735-62187754 MS.gene57537:CDS 35.0%
ATCAAGAGATGATTACTTGG+TGG - chr8.3:62187265-62187284 MS.gene57537:CDS 35.0%
CATCTTGTTAAACTTGCACA+CGG + chr8.3:62187872-62187891 None:intergenic 35.0%
CCAAGTAATCATCTCTTGAT+TGG + chr8.3:62187265-62187284 None:intergenic 35.0%
CCAATCAAGAGATGATTACT+TGG - chr8.3:62187262-62187281 MS.gene57537:CDS 35.0%
CTTTAGTGAAAATCAGCTTC+GGG - chr8.3:62186671-62186690 MS.gene57537:CDS 35.0%
GAAATTAAAGGTTCATGCAG+AGG - chr8.3:62187101-62187120 MS.gene57537:CDS 35.0%
GAATCTGAATGCTTCTGAAT+TGG - chr8.3:62187811-62187830 MS.gene57537:CDS 35.0%
GCTTTAGTGAAAATCAGCTT+CGG - chr8.3:62186670-62186689 MS.gene57537:CDS 35.0%
GTCAGGAAAGTAGAAAAGTA+CGG + chr8.3:62187067-62187086 None:intergenic 35.0%
TAACCATGATTCTGTTTCAC+TGG - chr8.3:62187040-62187059 MS.gene57537:CDS 35.0%
TCAAATGCGTTTGTAAGTCA+TGG - chr8.3:62186889-62186908 MS.gene57537:CDS 35.0%
TCGATGATATGCAAGTGATT+TGG + chr8.3:62187216-62187235 None:intergenic 35.0%
TGGTCATAACCAAAACCATA+TGG + chr8.3:62187245-62187264 None:intergenic 35.0%
TTCCTATATTGATGTTTCCG+CGG - chr8.3:62186947-62186966 MS.gene57537:CDS 35.0%
! ATGATATGCAAGTGATTTGG+CGG + chr8.3:62187213-62187232 None:intergenic 35.0%
!! GTTGATTTTGACCACAATCT+TGG - chr8.3:62187536-62187555 MS.gene57537:intron 35.0%
GGTGATATTGTTGGAACTGA+TGG - chr8.3:62187731-62187750 MS.gene57537:CDS 40.0%
GTGCAAGAGGAGAATAAACA+TGG - chr8.3:62186782-62186801 MS.gene57537:CDS 40.0%
GTTCAACAAACCTCTACGTA+TGG - chr8.3:62187141-62187160 MS.gene57537:CDS 40.0%
TACTCATTCAAATCCTGCTG+AGG - chr8.3:62186841-62186860 MS.gene57537:CDS 40.0%
TATCATCGACGTCAACCATA+TGG - chr8.3:62187227-62187246 MS.gene57537:CDS 40.0%
TCTATAGAGCGAATTCGAAC+AGG + chr8.3:62187005-62187024 None:intergenic 40.0%
TCTTCAATGGGGCTATTCAT+TGG - chr8.3:62187426-62187445 MS.gene57537:intron 40.0%
TGGTCAAAATCAACTGGAAG+AGG + chr8.3:62187530-62187549 None:intergenic 40.0%
TTCCGCGGAAACATCAATAT+AGG + chr8.3:62186952-62186971 None:intergenic 40.0%
! AAGATGGATTCCATACGTAG+AGG + chr8.3:62187154-62187173 None:intergenic 40.0%
! AGCTTCGGAATGATGCTTTT+CGG - chr8.3:62186696-62186715 MS.gene57537:CDS 40.0%
! ATATAAGGGAAGAGCACAGA+AGG + chr8.3:62187697-62187716 None:intergenic 40.0%
!! CTGATTTTCACTAAAGCACC+TGG + chr8.3:62186667-62186686 None:intergenic 40.0%
!! TAATCCTGCTTTGGAAGCTT+TGG + chr8.3:62187405-62187424 None:intergenic 40.0%
AACTGATGGTTGGAGTGGAT+TGG - chr8.3:62187745-62187764 MS.gene57537:CDS 45.0%
AAGCGGAGACTCTGTATACA+CGG + chr8.3:62187837-62187856 None:intergenic 45.0%
AATCAGCTTCGGGATAGCTT+CGG - chr8.3:62186681-62186700 MS.gene57537:CDS 45.0%
ATTCTGTTGCGAGTAACCCT+GGG + chr8.3:62186755-62186774 None:intergenic 45.0%
CAATGGGGCTATTCATTGGT+TGG - chr8.3:62187430-62187449 MS.gene57537:intron 45.0%
CAGAATCAAGAACGTGCAAG+AGG - chr8.3:62186769-62186788 MS.gene57537:CDS 45.0%
CCTTAGCGAGAACTTCATCA+CGG - chr8.3:62187297-62187316 MS.gene57537:CDS 45.0%
GGTCCAGTGAAACAGAATCA+TGG + chr8.3:62187046-62187065 None:intergenic 45.0%
GTGGCATGGCACATAAAACA+GGG - chr8.3:62186625-62186644 MS.gene57537:CDS 45.0%
TATGCCAAAGCTTCCAAAGC+AGG - chr8.3:62187398-62187417 MS.gene57537:intron 45.0%
TTAAACTTGCACACGGTCCA+GGG + chr8.3:62187865-62187884 None:intergenic 45.0%
TTGGCGGCAAAAGAAGACAA+AGG + chr8.3:62187197-62187216 None:intergenic 45.0%
! CACTAAAGCACCTGGATGTA+TGG + chr8.3:62186659-62186678 None:intergenic 45.0%
! CGACGTCAACCATATGGTTT+TGG - chr8.3:62187233-62187252 MS.gene57537:CDS 45.0%
! GCTTCGGAATGATGCTTTTC+GGG - chr8.3:62186697-62186716 MS.gene57537:CDS 45.0%
! GGAAGCTTTGGCATAATCCA+TGG + chr8.3:62187393-62187412 None:intergenic 45.0%
ACTTTACTGGCAACCTCAGC+AGG + chr8.3:62186857-62186876 None:intergenic 50.0%
AGCGAGAACTTCATCACGGT+TGG - chr8.3:62187301-62187320 MS.gene57537:CDS 50.0%
CACCTGGATGTATGGCAAGT+GGG + chr8.3:62186651-62186670 None:intergenic 50.0%
CCGTGATGAAGTTCTCGCTA+AGG + chr8.3:62187300-62187319 None:intergenic 50.0%
GATTCTGTTGCGAGTAACCC+TGG + chr8.3:62186756-62186775 None:intergenic 50.0%
GGTGGCATGGCACATAAAAC+AGG - chr8.3:62186624-62186643 MS.gene57537:CDS 50.0%
GTTAAACTTGCACACGGTCC+AGG + chr8.3:62187866-62187885 None:intergenic 50.0%
GTTGGAACTGATGGTTGGAG+TGG - chr8.3:62187740-62187759 MS.gene57537:CDS 50.0%
TCATCACTTGGAATCCCCCA+GGG - chr8.3:62186736-62186755 MS.gene57537:CDS 50.0%
TTCATCACTTGGAATCCCCC+AGG - chr8.3:62186735-62186754 MS.gene57537:CDS 50.0%
TTCTGTTGCGAGTAACCCTG+GGG + chr8.3:62186754-62186773 None:intergenic 50.0%
GCACCTGGATGTATGGCAAG+TGG + chr8.3:62186652-62186671 None:intergenic 55.0%
TCTGTTGCGAGTAACCCTGG+GGG + chr8.3:62186753-62186772 None:intergenic 55.0%
CACGGTCCAGGGAGTGAAAG+CGG + chr8.3:62187854-62187873 None:intergenic 60.0%
GAGTCTCCGCTTTCACTCCC+TGG - chr8.3:62187845-62187864 MS.gene57537:CDS 60.0%
GGCCCACTTGCCATACATCC+AGG - chr8.3:62186646-62186665 MS.gene57537:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 62186621 62187892 62186621 ID=MS.gene57537
chr8.3 mRNA 62186621 62187892 62186621 ID=MS.gene57537.t1;Parent=MS.gene57537
chr8.3 exon 62187613 62187892 62187613 ID=MS.gene57537.t1.exon1;Parent=MS.gene57537.t1
chr8.3 CDS 62187613 62187892 62187613 ID=cds.MS.gene57537.t1;Parent=MS.gene57537.t1
chr8.3 exon 62186621 62187402 62186621 ID=MS.gene57537.t1.exon2;Parent=MS.gene57537.t1
chr8.3 CDS 62186621 62187402 62186621 ID=cds.MS.gene57537.t1;Parent=MS.gene57537.t1
Gene Sequence

>MS.gene57537

ATGGGTGGCATGGCACATAAAACAGGGCCCACTTGCCATACATCCAGGTGCTTTAGTGAAAATCAGCTTCGGGATAGCTTCGGAATGATGCTTTTCGGGACATTTCCTATATATTTCATCACTTGGAATCCCCCAGGGTTACTCGCAACAGAATCAAGAACGTGCAAGAGGAGAATAAACATGGCGAAGATGAATGCTACTTTTAATCTTCCTGCTGAATTACTCATTCAAATCCTGCTGAGGTTGCCAGTAAAGTATCTTTTACGTTTCAAATGCGTTTGTAAGTCATGGTTTTCTCTGATCTCTTTTGATCCTCACTTTGCGAATTCCTATATTGATGTTTCCGCGGAAAGAATTGTGTTAATATCAAGAACTCTATCTCCTGTTCGAATTCGCTCTATAGATTTTGAAACATCCATTAACCATGATTCTGTTTCACTGGACCGTACTTTTCTACTTTCCTGACGTTATTCTTCTTATGAAATTAAAGGTTCATGCAGAGGGTTTATATTTTTGGACTGTTCAACAAACCTCTACGTATGGAATCCATCTTCTAGATTTCACAAAGAAATACCTTTGTCTTCTTTTGCCGCCAAATCACTTGCATATCATCGACGTCAACCATATGGTTTTGGTTATGACCAATCAAGAGATGATTACTTGGTGGTTTTATTGTCCTTAGCGAGAACTTCATCACGGTTGGAGTTTTTCTCATTGAGAGATAATAAGTGGAATGAAATTGAATGTCCCCAATTCACTTATTTGAATACCATGGATTATGCCAAAGCTTCCAAAGCAGGATTATTCTTCAATGGGGCTATTCATTGGTTGGCTTCTCATTATCATGATATGCGAGTGAAAGTTATTGTTGTCTTTGATTTAATGGAAAGGAAACTGTTACAGATACCTCTTCCAGTTGATTTTGACCACAATCTTGGGTATTATGATTTGTGGGTATTTGGAAAATTTCTCAGTCTATGGAAAAGAAATTTTCATAATTGGACAATTGAAATATGGGTGATGAAGGAATACAAACAACATTCGTCTTGGACTAAGTCTCTTGTAATTTCTAGCCTTCTGTGCTCTTCCCTTATATACTCTACAAAAAGTGGTGATATTGTTGGAACTGATGGTTGGAGTGGATTGGTGAAGTTTAATGATAAAGGACAGCAGATAGAGTATCTCGAAATGAATCTGAATGCTTCTGAATTGGCCGTGTATACAGAGTCTCCGCTTTCACTCCCTGGACCGTGTGCAAGTTTAACAAGATGA
Protein sequence

>MS.gene57537.t1

MGGMAHKTGPTCHTSRCFSENQLRDSFGMMLFGTFPIYFITWNPPGLLATESRTCKRRINMAKMNATFNLPAELLIQILLRLPVKYLLRFKCVCSCRGFIFLDCSTNLYVWNPSSRFHKEIPLSSFAAKSLAYHRRQPYGFGYDQSRDDYLVVLLSLARTSSRLEFFSLRDNKWNEIECPQFTYLNTMDYAKASKAGLFFNGAIHWLASHYHDMRVKVIVVFDLMERKLLQIPLPVDFDHNLGYYDLWVFGKFLSLWKRNFHNWTIEIWVMKEYKQHSSWTKSLVISSLLCSSLIYSTKSGDIVGTDGWSGLVKFNDKGQQIEYLEMNLNASELAVYTESPLSLPGPCASLTR