AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035238


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035238.t1 MTR_4g117780 57.658 222 83 6 3 216 149 367 6.33e-70 218
MS.gene035238.t1 MTR_4g117770 57.658 222 83 6 3 216 149 367 1.59e-67 211
MS.gene035238.t1 MTR_4g117810 57.658 222 83 6 3 216 149 367 1.59e-67 211
MS.gene035238.t1 MTR_6g060650 55.357 224 81 6 3 215 157 372 3.14e-66 208
MS.gene035238.t1 MTR_4g023130 55.505 218 83 6 3 215 104 312 4.78e-65 204
MS.gene035238.t1 MTR_4g117850 55.556 225 76 7 3 209 155 373 1.65e-64 204
MS.gene035238.t1 MTR_4g117720 54.741 232 79 9 3 216 155 378 7.09e-64 202
MS.gene035238.t1 MTR_3g034180 54.077 233 80 6 3 215 263 488 9.43e-64 205
MS.gene035238.t1 MTR_4g023080 54.587 218 85 6 3 215 145 353 1.57e-63 201
MS.gene035238.t1 MTR_3g103020 53.947 228 85 7 3 216 157 378 9.22e-63 199
MS.gene035238.t1 MTR_8g040935 56.585 205 77 6 18 216 10 208 2.03e-62 193
MS.gene035238.t1 MTR_4g109280 57.500 200 74 6 20 216 168 359 7.85e-62 197
MS.gene035238.t1 MTR_4g109270 54.545 220 89 5 3 216 150 364 9.87e-62 197
MS.gene035238.t1 MTR_3g034160 53.448 232 83 5 3 215 149 374 4.35e-59 190
MS.gene035238.t1 MTR_4g116330 52.212 226 89 7 3 216 162 380 7.35e-59 190
MS.gene035238.t1 MTR_4g118380 55.172 203 72 5 3 190 159 357 1.12e-58 189
MS.gene035238.t1 MTR_4g116320 55.941 202 74 7 17 213 64 255 7.41e-58 183
MS.gene035238.t1 MTR_4g122850 50.877 228 92 8 3 216 143 364 2.65e-56 183
MS.gene035238.t1 MTR_4g021785 50.685 219 95 6 3 216 145 355 3.94e-56 182
MS.gene035238.t1 MTR_1g055135 49.565 230 97 6 3 216 149 375 8.86e-56 182
MS.gene035238.t1 MTR_3g034130 54.106 207 70 5 3 190 149 349 2.20e-55 181
MS.gene035238.t1 MTR_1g055175 51.244 201 84 7 22 216 9 201 9.76e-55 174
MS.gene035238.t1 MTR_4g023090 46.957 230 94 6 3 211 157 379 2.18e-54 178
MS.gene035238.t1 MTR_4g023070 47.917 240 89 9 3 215 139 369 5.19e-54 177
MS.gene035238.t1 MTR_4g118400 53.886 193 66 6 20 210 123 294 1.08e-53 174
MS.gene035238.t1 MTR_4g023380 47.059 238 87 8 3 216 157 379 8.34e-52 171
MS.gene035238.t1 MTR_4g094365 50.222 225 93 9 3 216 148 364 1.50e-51 170
MS.gene035238.t1 MTR_5g097210 50.885 226 84 10 3 211 143 358 4.29e-51 169
MS.gene035238.t1 MTR_4g023120 45.643 241 95 9 3 216 139 370 1.40e-48 163
MS.gene035238.t1 MTR_8g433030 52.308 195 84 6 20 212 151 338 2.10e-48 162
MS.gene035238.t1 MTR_8g032390 52.308 195 84 6 20 212 184 371 6.04e-48 161
MS.gene035238.t1 MTR_1g055195 49.778 225 94 9 3 216 262 478 7.68e-48 164
MS.gene035238.t1 MTR_6g463430 48.357 213 87 10 14 216 3 202 2.22e-47 155
MS.gene035238.t1 MTR_1g055165 51.765 170 72 5 50 216 156 318 1.18e-46 156
MS.gene035238.t1 MTR_4g023190 47.727 220 98 7 3 214 147 357 1.35e-46 157
MS.gene035238.t1 MTR_4g023200 47.273 220 100 7 3 214 147 358 2.26e-46 157
MS.gene035238.t1 MTR_5g070080 49.776 223 93 8 3 213 167 382 4.45e-46 157
MS.gene035238.t1 MTR_4g023150 47.273 220 100 7 3 214 147 358 2.68e-45 154
MS.gene035238.t1 MTR_8g433040 46.087 230 87 10 3 216 156 364 4.44e-43 148
MS.gene035238.t1 MTR_8g032380 46.087 230 87 10 3 216 156 364 4.44e-43 148
MS.gene035238.t1 MTR_4g117530 45.413 218 77 7 3 216 74 253 4.51e-43 145
MS.gene035238.t1 MTR_4g118370 46.429 224 80 10 3 211 57 255 1.75e-42 144
MS.gene035238.t1 MTR_1g055250 45.098 204 93 7 1 193 49 244 4.09e-42 145
MS.gene035238.t1 MTR_4g117750 45.778 225 100 9 4 210 133 353 4.90e-40 143
MS.gene035238.t1 MTR_4g117750 60.185 108 38 4 107 211 373 478 1.41e-30 117
MS.gene035238.t1 MTR_5g070088 44.493 227 105 8 3 214 163 383 2.11e-39 139
MS.gene035238.t1 MTR_7g079400 40.928 237 111 9 1 216 109 337 4.06e-39 137
MS.gene035238.t1 MTR_8g102520 41.631 233 111 9 1 216 98 322 6.58e-39 136
MS.gene035238.t1 MTR_4g117870 43.363 226 73 8 3 216 85 267 3.31e-38 133
MS.gene035238.t1 MTR_5g070130 42.358 229 110 9 3 213 149 373 9.75e-38 135
MS.gene035238.t1 MTR_4g023440 42.986 221 73 7 4 216 157 332 7.39e-37 131
MS.gene035238.t1 MTR_4g116330 42.478 226 72 7 3 216 162 341 1.35e-35 128
MS.gene035238.t1 MTR_4g118360 56.818 132 48 5 88 216 1 126 1.03e-34 120
MS.gene035238.t1 MTR_2g048580 35.747 221 119 8 3 216 138 342 5.78e-31 116
MS.gene035238.t1 MTR_7g079370 36.325 234 112 9 3 212 132 352 4.98e-30 114
MS.gene035238.t1 MTR_4g117430 51.240 121 46 4 3 118 42 154 2.12e-29 107
MS.gene035238.t1 MTR_4g121580 44.103 195 63 9 3 184 157 318 4.28e-29 112
MS.gene035238.t1 MTR_8g098830 41.765 170 86 6 21 186 34 194 9.81e-29 107
MS.gene035238.t1 MTR_5g023000 60.000 95 36 2 123 216 6 99 9.92e-28 101
MS.gene035238.t1 MTR_4g122860 39.594 197 79 8 27 216 157 320 4.38e-26 103
MS.gene035238.t1 MTR_8g013010 53.226 124 38 5 3 116 196 309 6.85e-26 102
MS.gene035238.t1 MTR_4g114700 36.199 221 72 6 3 207 51 218 2.04e-23 94.4
MS.gene035238.t1 MTR_7g079420 36.527 167 97 5 53 216 984 1144 1.66e-22 95.5
MS.gene035238.t1 MTR_7g079450 42.466 146 72 5 73 216 2 137 2.73e-21 85.9
MS.gene035238.t1 MTR_7g117720 55.172 87 35 1 129 211 186 272 4.75e-18 80.5
MS.gene035238.t1 MTR_4g023430 50.602 83 36 2 138 216 141 222 1.09e-15 73.2
MS.gene035238.t1 MTR_8g013005 68.333 60 17 2 158 216 1 59 2.34e-15 68.2
MS.gene035238.t1 MTR_6g034720 44.000 100 42 3 3 90 73 170 8.73e-15 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 50 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAAGTTTGATGGAATATA+AGG 0.192370 8.1:+9555107 None:intergenic
GATTGTGGTTTGTGGGTATT+TGG 0.221112 8.1:-9554843 MS.gene035238:CDS
ATCGTAATACAACTGAAATA+TGG 0.303594 8.1:-9554791 MS.gene035238:CDS
GAGTAGGGTCCCTTTGTAAT+GGG 0.322096 8.1:-9554998 MS.gene035238:CDS
TTTGTAATGGGGCTATTCAT+TGG 0.336970 8.1:-9554986 MS.gene035238:CDS
CTAATGATGTCCTTTACATT+TGG 0.354400 8.1:-9554716 MS.gene035238:CDS
CCCTTATATTCCATCAAACT+TGG 0.358101 8.1:-9555108 MS.gene035238:CDS
GACTCTTTGCTTTCACTCCC+TGG 0.364558 8.1:-9554543 MS.gene035238:CDS
GGAGTAGGGTCCCTTTGTAA+TGG 0.396390 8.1:-9554999 MS.gene035238:CDS
TTTCCAGATGATTATTATAA+CGG 0.407070 8.1:-9554885 MS.gene035238:CDS
AGTCCCGTTGATGATTATTC+CGG 0.422801 8.1:-9555020 MS.gene035238:CDS
AGAGCATTGCTCCAATTGTA+AGG 0.429098 8.1:-9554595 MS.gene035238:CDS
TAATGGGGCTATTCATTGGT+TGG 0.429474 8.1:-9554982 MS.gene035238:CDS
TCGTAATACAACTGAAATAT+GGG 0.435341 8.1:-9554790 MS.gene035238:CDS
ACCACGTGAATCCTTACAAT+TGG 0.439030 8.1:+9554584 None:intergenic
AAACCGTTATAATAATCATC+TGG 0.454919 8.1:+9554882 None:intergenic
CCAAGTTTGATGGAATATAA+GGG 0.458503 8.1:+9555108 None:intergenic
ACGTATGGATTGTGGTTTGT+GGG 0.472010 8.1:-9554850 MS.gene035238:CDS
AGATGGTGGTACAGAAAGGT+TGG 0.484999 8.1:-9554646 MS.gene035238:CDS
GTTGATGATTATTCCGGAGT+AGG 0.486016 8.1:-9555014 MS.gene035238:CDS
ACTCCGGAATAATCATCAAC+GGG 0.493191 8.1:+9555017 None:intergenic
CTACTACAAGGGGAGTGATC+AGG 0.499364 8.1:+9555041 None:intergenic
TTTCCGTTGATTTAACAGAA+AGG 0.499674 8.1:-9554926 MS.gene035238:CDS
TTTCCTTTCTGTTAAATCAA+CGG 0.503324 8.1:+9554923 None:intergenic
AACGTATGGATTGTGGTTTG+TGG 0.511387 8.1:-9554851 MS.gene035238:CDS
AATACAAAGTGAATTCGTCT+TGG 0.512787 8.1:-9554758 MS.gene035238:CDS
TTGATGATTATTCCGGAGTA+GGG 0.518500 8.1:-9555013 MS.gene035238:CDS
TAAGCTTGCTCATTGCCACC+AGG 0.520225 8.1:+9554525 None:intergenic
ATTACAAAGGGACCCTACTC+CGG 0.531948 8.1:+9555001 None:intergenic
GGGAAAAGTACCAAATGTAA+AGG 0.532919 8.1:+9554706 None:intergenic
ATTTCTCAGTCTATGCGCTA+AGG 0.533303 8.1:-9554817 MS.gene035238:CDS
TCTCATTGAGAGACAATACA+TGG 0.536974 8.1:-9555079 MS.gene035238:CDS
TTTCTCAGTCTATGCGCTAA+GGG 0.541350 8.1:-9554816 MS.gene035238:CDS
ATTTGTGAACGTATGGATTG+TGG 0.541400 8.1:-9554858 MS.gene035238:CDS
GGTGATATTATTGGAAGAGA+TGG 0.541574 8.1:-9554663 MS.gene035238:CDS
TCATCAACGGGACTACTACA+AGG 0.551058 8.1:+9555029 None:intergenic
ATGAATAGCCCCATTACAAA+GGG 0.555543 8.1:+9554989 None:intergenic
GAAGAGATGGTGGTACAGAA+AGG 0.566869 8.1:-9554650 MS.gene035238:CDS
CTGATCATCTCCAAAACCAT+AGG 0.587717 8.1:+9555148 None:intergenic
TACTCCGGAATAATCATCAA+CGG 0.590143 8.1:+9555016 None:intergenic
AATGAATAGCCCCATTACAA+AGG 0.597680 8.1:+9554988 None:intergenic
CATCAACGGGACTACTACAA+GGG 0.609440 8.1:+9555030 None:intergenic
GATATTATTGGAAGAGATGG+TGG 0.612062 8.1:-9554660 MS.gene035238:CDS
AAGCTTGCTCATTGCCACCA+GGG 0.612937 8.1:+9554526 None:intergenic
TCTTTGCTTTCACTCCCTGG+TGG 0.621264 8.1:-9554540 MS.gene035238:CDS
AATTGTAAGGATTCACGTGG+TGG 0.626284 8.1:-9554582 MS.gene035238:CDS
AGTAGGGTCCCTTTGTAATG+GGG 0.631345 8.1:-9554997 MS.gene035238:CDS
ATCAACGGGACTACTACAAG+GGG 0.651675 8.1:+9555031 None:intergenic
TCCAATTGTAAGGATTCACG+TGG 0.697098 8.1:-9554585 MS.gene035238:CDS
TTGGTGAAGTATGATGACAA+TGG 0.748686 8.1:-9554627 MS.gene035238:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCCAGATGATTATTATAA+CGG - chr8.1:9554791-9554810 MS.gene035238:CDS 20.0%
!!! TTTTCGTGATGATTTAAAAA+AGG - chr8.1:9554718-9554737 MS.gene035238:CDS 20.0%
! AAACCGTTATAATAATCATC+TGG + chr8.1:9554797-9554816 None:intergenic 25.0%
! ACAAAAAGTGGTGATATTAT+TGG - chr8.1:9555004-9555023 MS.gene035238:CDS 25.0%
! ATCGTAATACAACTGAAATA+TGG - chr8.1:9554885-9554904 MS.gene035238:CDS 25.0%
! TCGTAATACAACTGAAATAT+GGG - chr8.1:9554886-9554905 MS.gene035238:CDS 25.0%
! TTTCCTTTCTGTTAAATCAA+CGG + chr8.1:9554756-9554775 None:intergenic 25.0%
!! ACTTTTTGTACAACAAATTG+GGG + chr8.1:9554993-9555012 None:intergenic 25.0%
!!! CACTTTTTGTACAACAAATT+GGG + chr8.1:9554994-9555013 None:intergenic 25.0%
AATACAAAGTGAATTCGTCT+TGG - chr8.1:9554918-9554937 MS.gene035238:CDS 30.0%
CCAAGTTTGATGGAATATAA+GGG + chr8.1:9554571-9554590 None:intergenic 30.0%
CTAATGATGTCCTTTACATT+TGG - chr8.1:9554960-9554979 MS.gene035238:CDS 30.0%
TCCAAGTTTGATGGAATATA+AGG + chr8.1:9554572-9554591 None:intergenic 30.0%
!! CCAATTTGTTGTACAAAAAG+TGG - chr8.1:9554992-9555011 MS.gene035238:CDS 30.0%
!! TTTCCGTTGATTTAACAGAA+AGG - chr8.1:9554750-9554769 MS.gene035238:CDS 30.0%
!!! CCACTTTTTGTACAACAAAT+TGG + chr8.1:9554995-9555014 None:intergenic 30.0%
AATGAATAGCCCCATTACAA+AGG + chr8.1:9554691-9554710 None:intergenic 35.0%
ATGAATAGCCCCATTACAAA+GGG + chr8.1:9554690-9554709 None:intergenic 35.0%
CCCTTATATTCCATCAAACT+TGG - chr8.1:9554568-9554587 MS.gene035238:CDS 35.0%
GAGAAAAACTCCAAGTTTGA+TGG + chr8.1:9554581-9554600 None:intergenic 35.0%
GATATTATTGGAAGAGATGG+TGG - chr8.1:9555016-9555035 MS.gene035238:CDS 35.0%
GGTGATATTATTGGAAGAGA+TGG - chr8.1:9555013-9555032 MS.gene035238:CDS 35.0%
TACTCCGGAATAATCATCAA+CGG + chr8.1:9554663-9554682 None:intergenic 35.0%
TCTCATTGAGAGACAATACA+TGG - chr8.1:9554597-9554616 MS.gene035238:CDS 35.0%
TTGGTGAAGTATGATGACAA+TGG - chr8.1:9555049-9555068 MS.gene035238:CDS 35.0%
! ATTTGTGAACGTATGGATTG+TGG - chr8.1:9554818-9554837 MS.gene035238:CDS 35.0%
! TTGATGATTATTCCGGAGTA+GGG - chr8.1:9554663-9554682 MS.gene035238:CDS 35.0%
! TTTGTAATGGGGCTATTCAT+TGG - chr8.1:9554690-9554709 MS.gene035238:CDS 35.0%
!! GGGAAAAGTACCAAATGTAA+AGG + chr8.1:9554973-9554992 None:intergenic 35.0%
!!! CGGTTTTATTTGTGAACGTA+TGG - chr8.1:9554811-9554830 MS.gene035238:CDS 35.0%
AATTGTAAGGATTCACGTGG+TGG - chr8.1:9555094-9555113 MS.gene035238:CDS 40.0%
ACCACGTGAATCCTTACAAT+TGG + chr8.1:9555095-9555114 None:intergenic 40.0%
ACTCCGGAATAATCATCAAC+GGG + chr8.1:9554662-9554681 None:intergenic 40.0%
ATTTCTCAGTCTATGCGCTA+AGG - chr8.1:9554859-9554878 MS.gene035238:CDS 40.0%
CTGATCATCTCCAAAACCAT+AGG + chr8.1:9554531-9554550 None:intergenic 40.0%
TAATGGGGCTATTCATTGGT+TGG - chr8.1:9554694-9554713 MS.gene035238:CDS 40.0%
TCCAATTGTAAGGATTCACG+TGG - chr8.1:9555091-9555110 MS.gene035238:CDS 40.0%
TTTCTCAGTCTATGCGCTAA+GGG - chr8.1:9554860-9554879 MS.gene035238:CDS 40.0%
! AACGTATGGATTGTGGTTTG+TGG - chr8.1:9554825-9554844 MS.gene035238:CDS 40.0%
! ACGTATGGATTGTGGTTTGT+GGG - chr8.1:9554826-9554845 MS.gene035238:CDS 40.0%
! GATTGTGGTTTGTGGGTATT+TGG - chr8.1:9554833-9554852 MS.gene035238:CDS 40.0%
!! AGAGCATTGCTCCAATTGTA+AGG - chr8.1:9555081-9555100 MS.gene035238:CDS 40.0%
!! AGTCCCGTTGATGATTATTC+CGG - chr8.1:9554656-9554675 MS.gene035238:CDS 40.0%
!! GTTGATGATTATTCCGGAGT+AGG - chr8.1:9554662-9554681 MS.gene035238:CDS 40.0%
AGTAGGGTCCCTTTGTAATG+GGG - chr8.1:9554679-9554698 MS.gene035238:CDS 45.0%
ATCAACGGGACTACTACAAG+GGG + chr8.1:9554648-9554667 None:intergenic 45.0%
ATTACAAAGGGACCCTACTC+CGG + chr8.1:9554678-9554697 None:intergenic 45.0%
CATCAACGGGACTACTACAA+GGG + chr8.1:9554649-9554668 None:intergenic 45.0%
GAGTAGGGTCCCTTTGTAAT+GGG - chr8.1:9554678-9554697 MS.gene035238:CDS 45.0%
TCATCAACGGGACTACTACA+AGG + chr8.1:9554650-9554669 None:intergenic 45.0%
! AGATGGTGGTACAGAAAGGT+TGG - chr8.1:9555030-9555049 MS.gene035238:CDS 45.0%
! GAAGAGATGGTGGTACAGAA+AGG - chr8.1:9555026-9555045 MS.gene035238:CDS 45.0%
CTACTACAAGGGGAGTGATC+AGG + chr8.1:9554638-9554657 None:intergenic 50.0%
GACTCTTTGCTTTCACTCCC+TGG - chr8.1:9555133-9555152 MS.gene035238:CDS 50.0%
GGAGTAGGGTCCCTTTGTAA+TGG - chr8.1:9554677-9554696 MS.gene035238:CDS 50.0%
TCTTTGCTTTCACTCCCTGG+TGG - chr8.1:9555136-9555155 MS.gene035238:CDS 50.0%
! AAGCTTGCTCATTGCCACCA+GGG + chr8.1:9555153-9555172 None:intergenic 50.0%
! TAAGCTTGCTCATTGCCACC+AGG + chr8.1:9555154-9555173 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 9554524 9555174 9554524 ID=MS.gene035238
chr8.1 mRNA 9554524 9555174 9554524 ID=MS.gene035238.t1;Parent=MS.gene035238
chr8.1 exon 9554524 9555174 9554524 ID=MS.gene035238.t1.exon1;Parent=MS.gene035238.t1
chr8.1 CDS 9554524 9555174 9554524 ID=cds.MS.gene035238.t1;Parent=MS.gene035238.t1
Gene Sequence

>MS.gene035238

ATGTCCTATGGTTTTGGAGATGATCAGTCAACAAATCATTACATCCCTTATATTCCATCAAACTTGGAGTTTTTCTCATTGAGAGACAATACATGGAAAGAAATTGAGTGTCCTGATCACTCCCCTTGTAGTAGTCCCGTTGATGATTATTCCGGAGTAGGGTCCCTTTGTAATGGGGCTATTCATTGGTTGGCTTTTCGTGATGATTTAAAAAAGGATGTTATTGTTTCCGTTGATTTAACAGAAAGGAAACTTTTAGATATAGATTTTCCAGATGATTATTATAACGGTTTTATTTGTGAACGTATGGATTGTGGTTTGTGGGTATTTGGAGAATTTCTCAGTCTATGCGCTAAGGGTTATCGTAATACAACTGAAATATGGGTGATGAAAGAATACAAAGTGAATTCGTCTTGGACTAAGACTCATGTTTTTTCTAATGATGTCCTTTACATTTGGTACTTTTCCCCAATTTGTTGTACAAAAAGTGGTGATATTATTGGAAGAGATGGTGGTACAGAAAGGTTGGTGAAGTATGATGACAATGGACAGTTGCTAGAGCATTGCTCCAATTGTAAGGATTCACGTGGTGGATATACTATGTATACAGACTCTTTGCTTTCACTCCCTGGTGGCAATGAGCAAGCTTAA

Protein sequence

>MS.gene035238.t1

MSYGFGDDQSTNHYIPYIPSNLEFFSLRDNTWKEIECPDHSPCSSPVDDYSGVGSLCNGAIHWLAFRDDLKKDVIVSVDLTERKLLDIDFPDDYYNGFICERMDCGLWVFGEFLSLCAKGYRNTTEIWVMKEYKVNSSWTKTHVFSNDVLYIWYFSPICCTKSGDIIGRDGGTERLVKYDDNGQLLEHCSNCKDSRGGYTMYTDSLLSLPGGNEQA