AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene046958


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene046958.t1 MTR_5g023000 92.929 99 7 0 44 142 1 99 1.74e-64 192
MS.gene046958.t1 MTR_4g109270 65.493 142 49 0 1 142 223 364 8.70e-63 196
MS.gene046958.t1 MTR_4g117870 68.421 133 40 2 10 142 137 267 4.23e-61 189
MS.gene046958.t1 MTR_3g103020 61.972 142 54 0 1 142 237 378 2.94e-58 185
MS.gene046958.t1 MTR_6g060650 62.411 141 51 1 1 141 234 372 8.91e-57 181
MS.gene046958.t1 MTR_4g116330 60.563 142 54 1 3 142 239 380 2.08e-54 175
MS.gene046958.t1 MTR_4g109280 62.500 144 49 3 1 142 219 359 1.18e-53 172
MS.gene046958.t1 MTR_8g040935 58.741 143 57 1 2 142 66 208 1.64e-53 167
MS.gene046958.t1 MTR_3g034180 62.411 141 50 2 1 141 351 488 7.11e-52 171
MS.gene046958.t1 MTR_3g034160 61.972 142 49 3 1 141 237 374 3.45e-48 159
MS.gene046958.t1 MTR_4g117720 57.823 147 54 3 2 142 234 378 3.53e-48 159
MS.gene046958.t1 MTR_4g116320 59.286 140 53 2 2 139 118 255 3.79e-48 155
MS.gene046958.t1 MTR_8g433040 59.286 140 54 2 3 142 228 364 6.23e-48 158
MS.gene046958.t1 MTR_8g032380 59.286 140 54 2 3 142 228 364 6.23e-48 158
MS.gene046958.t1 MTR_4g023130 57.447 141 57 2 1 141 175 312 1.44e-47 155
MS.gene046958.t1 MTR_4g117780 57.639 144 58 1 2 142 224 367 1.59e-47 157
MS.gene046958.t1 MTR_4g023090 51.948 154 58 3 2 141 232 383 4.26e-47 156
MS.gene046958.t1 MTR_4g023380 51.829 164 53 4 3 142 218 379 1.10e-46 155
MS.gene046958.t1 MTR_4g117770 57.639 144 58 1 2 142 224 367 3.31e-46 154
MS.gene046958.t1 MTR_4g117810 57.639 144 58 1 2 142 224 367 3.31e-46 154
MS.gene046958.t1 MTR_4g117850 56.835 139 58 2 2 139 239 376 3.69e-46 154
MS.gene046958.t1 MTR_4g094365 53.472 144 64 2 1 142 222 364 2.34e-45 151
MS.gene046958.t1 MTR_8g433030 57.246 138 55 3 1 137 203 337 2.78e-45 150
MS.gene046958.t1 MTR_4g023080 54.610 141 61 2 1 141 216 353 4.11e-45 150
MS.gene046958.t1 MTR_4g021785 54.930 142 61 2 1 142 217 355 4.34e-45 150
MS.gene046958.t1 MTR_8g032390 57.246 138 55 3 1 137 236 370 4.84e-45 150
MS.gene046958.t1 MTR_4g122850 54.225 142 61 4 3 142 225 364 1.05e-44 150
MS.gene046958.t1 MTR_3g034130 56.250 128 54 1 1 128 237 362 1.07e-44 150
MS.gene046958.t1 MTR_6g463430 55.319 141 57 2 3 142 67 202 3.24e-44 144
MS.gene046958.t1 MTR_5g097210 56.934 137 54 3 3 138 227 359 3.44e-44 149
MS.gene046958.t1 MTR_5g070130 54.545 143 63 2 2 142 235 377 9.24e-44 148
MS.gene046958.t1 MTR_4g023070 52.597 154 58 3 1 141 218 369 2.20e-43 146
MS.gene046958.t1 MTR_5g070080 55.944 143 61 2 1 142 245 386 7.52e-43 145
MS.gene046958.t1 MTR_4g118360 63.793 116 36 4 29 142 15 126 1.73e-42 137
MS.gene046958.t1 MTR_4g118380 58.197 122 40 2 1 113 236 355 4.17e-41 140
MS.gene046958.t1 MTR_4g023120 50.968 155 61 3 1 142 218 370 8.28e-41 140
MS.gene046958.t1 MTR_1g055195 52.778 144 62 5 1 142 339 478 1.26e-40 141
MS.gene046958.t1 MTR_1g055175 52.113 142 63 4 3 142 63 201 2.17e-40 134
MS.gene046958.t1 MTR_4g116330 63.063 111 39 1 34 142 231 341 3.13e-40 137
MS.gene046958.t1 MTR_1g055165 52.113 142 63 4 3 142 180 318 2.43e-39 135
MS.gene046958.t1 MTR_4g117750 56.923 130 48 1 19 140 352 481 3.51e-39 137
MS.gene046958.t1 MTR_4g117750 47.917 144 62 5 3 138 217 355 3.95e-29 110
MS.gene046958.t1 MTR_4g023190 53.425 146 58 5 1 140 216 357 1.65e-38 133
MS.gene046958.t1 MTR_4g118370 51.538 130 59 2 10 139 132 257 7.77e-37 127
MS.gene046958.t1 MTR_4g023200 52.740 146 59 5 1 140 217 358 9.63e-37 129
MS.gene046958.t1 MTR_1g055135 50.345 145 64 4 3 142 234 375 1.18e-36 129
MS.gene046958.t1 MTR_5g070088 50.000 144 70 2 1 142 243 386 1.65e-36 129
MS.gene046958.t1 MTR_4g023150 51.370 146 61 5 1 140 217 358 5.76e-36 127
MS.gene046958.t1 MTR_4g118400 50.000 136 54 2 1 136 173 294 3.21e-35 123
MS.gene046958.t1 MTR_4g023440 60.377 106 40 1 37 142 229 332 2.79e-34 122
MS.gene046958.t1 MTR_4g117530 58.000 100 39 1 43 142 157 253 3.22e-34 120
MS.gene046958.t1 MTR_4g122860 50.000 128 57 2 19 142 196 320 3.64e-34 122
MS.gene046958.t1 MTR_7g079450 46.853 143 69 4 1 142 1 137 2.68e-32 111
MS.gene046958.t1 MTR_1g055250 48.031 127 61 3 3 129 132 253 6.24e-32 115
MS.gene046958.t1 MTR_8g102520 44.138 145 74 4 1 142 182 322 1.15e-30 112
MS.gene046958.t1 MTR_7g079400 45.270 148 70 4 2 142 194 337 4.44e-30 111
MS.gene046958.t1 MTR_7g117720 63.218 87 28 2 55 137 186 272 4.96e-27 102
MS.gene046958.t1 MTR_8g098830 46.957 115 54 4 1 113 87 196 9.56e-24 92.4
MS.gene046958.t1 MTR_7g079370 44.203 138 71 4 1 136 217 350 4.07e-23 92.8
MS.gene046958.t1 MTR_4g121580 47.107 121 52 4 13 132 230 339 6.24e-23 92.8
MS.gene046958.t1 MTR_4g122840 40.441 136 45 3 8 142 164 264 8.88e-23 90.5
MS.gene046958.t1 MTR_4g114700 53.608 97 40 2 40 133 124 218 1.73e-22 89.4
MS.gene046958.t1 MTR_7g079420 39.041 146 79 5 2 142 1004 1144 2.31e-21 89.4
MS.gene046958.t1 MTR_4g023430 50.617 81 37 1 65 142 142 222 2.11e-19 80.9
MS.gene046958.t1 MTR_8g013005 61.017 59 23 0 84 142 1 59 5.73e-19 75.5
MS.gene046958.t1 MTR_2g048580 38.194 144 74 5 2 142 211 342 3.40e-18 79.3
MS.gene046958.t1 MTR_4g023100 57.143 56 22 1 86 141 65 118 1.10e-13 63.5
MS.gene046958.t1 MTR_4g109290 58.491 53 22 0 90 142 136 188 4.62e-13 63.5
MS.gene046958.t1 MTR_2g064960 58.140 43 18 0 100 142 17 59 6.38e-11 54.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 27 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGTGATGTGTGGGTATT+TGG 0.202909 5.4:+258335 MS.gene046958:CDS
TTGGAGAATTTCTCAGTATA+TGG 0.237144 5.4:+258354 MS.gene046958:CDS
CTAATGATACACTTGAAATA+TGG 0.280671 5.4:+258390 MS.gene046958:CDS
GCTTCATAGTCAATATCAAT+TGG 0.392804 5.4:-258308 None:intergenic
TGGAGAATTTCTCAGTATAT+GGG 0.404173 5.4:+258355 MS.gene046958:CDS
TGCCTTTGATTTAATCAAAA+GGG 0.415367 5.4:+258265 MS.gene046958:CDS
TTGCCTTTGATTTAATCAAA+AGG 0.422457 5.4:+258264 MS.gene046958:CDS
AACAGAAGATGGATGGAAAT+TGG 0.423122 5.4:+258532 MS.gene046958:CDS
GGTGACAGTGACCAAGCTTA+AGG 0.452041 5.4:+258659 MS.gene046958:CDS
GAGTCTCTGCTTTCACTCCC+TGG 0.452167 5.4:+258638 MS.gene046958:CDS
AATACAAAGTGCATTCCTCT+TGG 0.461402 5.4:+258423 MS.gene046958:CDS
ATTTCTCAGTATATGGGCTA+TGG 0.479112 5.4:+258361 MS.gene046958:CDS
GATATCATTGGAACAGAAGA+TGG 0.479823 5.4:+258521 MS.gene046958:CDS
TATTGGGGAAAACAGCTTAG+TGG 0.491914 5.4:-258480 None:intergenic
TTGTGTCATCTCCTTAAGCT+TGG 0.494818 5.4:-258670 None:intergenic
TAATGATACACTTGAAATAT+GGG 0.496908 5.4:+258391 MS.gene046958:CDS
CTTCATAGTCAATATCAATT+GGG 0.498545 5.4:-258307 None:intergenic
TAAGCTTGGTCACTGTCACC+AGG 0.500905 5.4:-258656 None:intergenic
CTCTGTATAAAGAGCCACTT+CGG 0.548132 5.4:-258618 None:intergenic
TCATTGGAACAGAAGATGGA+TGG 0.571754 5.4:+258525 MS.gene046958:CDS
TACTATTCTGACAGTACACA+TGG 0.588984 5.4:+258593 MS.gene046958:CDS
AAGCTTGGTCACTGTCACCA+GGG 0.590911 5.4:-258655 None:intergenic
AGCTACAAATTGTGATGTGT+GGG 0.604681 5.4:+258328 MS.gene046958:CDS
AAGCTACAAATTGTGATGTG+TGG 0.622283 5.4:+258327 MS.gene046958:CDS
TTGGTGAAGTATAATGACAA+AGG 0.632256 5.4:+258551 MS.gene046958:CDS
ACAAAGGACAGTTGATAGAG+TGG 0.633105 5.4:+258567 MS.gene046958:CDS
CAGTACACATGGAACCGAAG+TGG 0.667775 5.4:+258604 MS.gene046958:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGATACACTTGAAATAT+GGG + chr5.4:258391-258410 MS.gene046958:CDS 20.0%
! CTAATGATACACTTGAAATA+TGG + chr5.4:258390-258409 MS.gene046958:CDS 25.0%
! CTTCATAGTCAATATCAATT+GGG - chr5.4:258310-258329 None:intergenic 25.0%
!! ACTTTTTGTAGAACATATTG+GGG - chr5.4:258498-258517 None:intergenic 25.0%
!! TGCCTTTGATTTAATCAAAA+GGG + chr5.4:258265-258284 MS.gene046958:CDS 25.0%
!! TTGCCTTTGATTTAATCAAA+AGG + chr5.4:258264-258283 MS.gene046958:CDS 25.0%
!!! CACTTTTTGTAGAACATATT+GGG - chr5.4:258499-258518 None:intergenic 25.0%
!!! TTCCCTTTTGATTAAATCAA+AGG - chr5.4:258270-258289 None:intergenic 25.0%
ACAAAAAGTGGTGATATCAT+TGG + chr5.4:258509-258528 MS.gene046958:CDS 30.0%
CCAATATGTTCTACAAAAAG+TGG + chr5.4:258497-258516 MS.gene046958:CDS 30.0%
GCTTCATAGTCAATATCAAT+TGG - chr5.4:258311-258330 None:intergenic 30.0%
TGGAGAATTTCTCAGTATAT+GGG + chr5.4:258355-258374 MS.gene046958:CDS 30.0%
TTGGAGAATTTCTCAGTATA+TGG + chr5.4:258354-258373 MS.gene046958:CDS 30.0%
TTGGTGAAGTATAATGACAA+AGG + chr5.4:258551-258570 MS.gene046958:CDS 30.0%
!!! ACTCTTGTTTTGTCTATTGA+CGG + chr5.4:258452-258471 MS.gene046958:CDS 30.0%
!!! CCACTTTTTGTAGAACATAT+TGG - chr5.4:258500-258519 None:intergenic 30.0%
AACAGAAGATGGATGGAAAT+TGG + chr5.4:258532-258551 MS.gene046958:CDS 35.0%
AAGCTACAAATTGTGATGTG+TGG + chr5.4:258327-258346 MS.gene046958:CDS 35.0%
AATACAAAGTGCATTCCTCT+TGG + chr5.4:258423-258442 MS.gene046958:CDS 35.0%
AATTGTGATGTGTGGGTATT+TGG + chr5.4:258335-258354 MS.gene046958:CDS 35.0%
AGCTACAAATTGTGATGTGT+GGG + chr5.4:258328-258347 MS.gene046958:CDS 35.0%
GATATCATTGGAACAGAAGA+TGG + chr5.4:258521-258540 MS.gene046958:CDS 35.0%
TACTATTCTGACAGTACACA+TGG + chr5.4:258593-258612 MS.gene046958:CDS 35.0%
! AACAAGAGTTTTTGTCCAAG+AGG - chr5.4:258441-258460 None:intergenic 35.0%
! ATTTCTCAGTATATGGGCTA+TGG + chr5.4:258361-258380 MS.gene046958:CDS 35.0%
CTCTGTATAAAGAGCCACTT+CGG - chr5.4:258621-258640 None:intergenic 40.0%
TATTGGGGAAAACAGCTTAG+TGG - chr5.4:258483-258502 None:intergenic 40.0%
TCATTGGAACAGAAGATGGA+TGG + chr5.4:258525-258544 MS.gene046958:CDS 40.0%
!! ACAAAGGACAGTTGATAGAG+TGG + chr5.4:258567-258586 MS.gene046958:CDS 40.0%
AAGCTTGGTCACTGTCACCA+GGG - chr5.4:258658-258677 None:intergenic 50.0%
CAGTACACATGGAACCGAAG+TGG + chr5.4:258604-258623 MS.gene046958:CDS 50.0%
TAAGCTTGGTCACTGTCACC+AGG - chr5.4:258659-258678 None:intergenic 50.0%
GAGTCTCTGCTTTCACTCCC+TGG + chr5.4:258638-258657 MS.gene046958:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 258251 258679 258251 ID=MS.gene046958
chr5.4 mRNA 258251 258679 258251 ID=MS.gene046958.t1;Parent=MS.gene046958
chr5.4 exon 258251 258679 258251 ID=MS.gene046958.t1.exon1;Parent=MS.gene046958.t1
chr5.4 CDS 258251 258679 258251 ID=cds.MS.gene046958.t1;Parent=MS.gene046958.t1
Gene Sequence

>MS.gene046958

ATGGATCTTATTCTTGCCTTTGATTTAATCAAAAGGGAACTTTTTGAGATTCATCTCCCAATTGATATTGACTATGAAGCTACAAATTGTGATGTGTGGGTATTTGGAGAATTTCTCAGTATATGGGCTATGGATTATGCTAATGATACACTTGAAATATGGGTGATGAAAGAATACAAAGTGCATTCCTCTTGGACAAAAACTCTTGTTTTGTCTATTGACGGCATTTCCACTAAGCTGTTTTCCCCAATATGTTCTACAAAAAGTGGTGATATCATTGGAACAGAAGATGGATGGAAATTGGTGAAGTATAATGACAAAGGACAGTTGATAGAGTGGCACTACTATTCTGACAGTACACATGGAACCGAAGTGGCTCTTTATACAGAGTCTCTGCTTTCACTCCCTGGTGACAGTGACCAAGCTTAA

Protein sequence

>MS.gene046958.t1

MDLILAFDLIKRELFEIHLPIDIDYEATNCDVWVFGEFLSIWAMDYANDTLEIWVMKEYKVHSSWTKTLVLSIDGISTKLFSPICSTKSGDIIGTEDGWKLVKYNDKGQLIEWHYYSDSTHGTEVALYTESLLSLPGDSDQA