AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57538


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57538.t1 MTR_8g433030 72.316 177 40 3 2 171 163 337 1.67e-83 250
MS.gene57538.t1 MTR_8g032390 72.316 177 40 3 2 171 196 370 4.03e-83 250
MS.gene57538.t1 MTR_8g433040 67.956 181 41 3 2 176 195 364 8.89e-78 236
MS.gene57538.t1 MTR_8g032380 67.956 181 41 3 2 176 195 364 8.89e-78 236
MS.gene57538.t1 MTR_6g060650 56.522 184 63 8 2 175 196 372 1.64e-56 182
MS.gene57538.t1 MTR_3g103020 55.851 188 65 8 2 176 196 378 2.90e-54 176
MS.gene57538.t1 MTR_4g094365 54.595 185 70 6 2 176 184 364 3.43e-52 171
MS.gene57538.t1 MTR_4g023130 53.226 186 65 8 2 175 137 312 1.12e-51 168
MS.gene57538.t1 MTR_4g109280 52.151 186 72 8 2 176 180 359 1.97e-51 169
MS.gene57538.t1 MTR_4g116320 54.396 182 68 7 1 172 78 254 8.76e-51 164
MS.gene57538.t1 MTR_4g023080 52.151 186 67 8 2 175 178 353 1.25e-49 164
MS.gene57538.t1 MTR_4g117720 54.688 192 63 10 2 176 194 378 4.85e-49 163
MS.gene57538.t1 MTR_4g122850 54.255 188 72 8 5 182 187 370 1.17e-48 162
MS.gene57538.t1 MTR_4g023070 49.246 199 66 8 2 175 181 369 3.90e-47 158
MS.gene57538.t1 MTR_4g021785 51.630 184 76 9 1 176 177 355 5.86e-47 157
MS.gene57538.t1 MTR_4g023090 50.251 199 69 9 2 176 194 386 8.44e-47 157
MS.gene57538.t1 MTR_4g109270 50.000 184 76 8 4 176 186 364 4.19e-46 155
MS.gene57538.t1 MTR_8g040935 48.936 188 82 6 1 176 23 208 1.20e-45 149
MS.gene57538.t1 MTR_3g034180 52.601 173 68 7 12 176 323 489 1.92e-45 156
MS.gene57538.t1 MTR_4g023380 49.275 207 67 10 2 176 179 379 1.11e-44 152
MS.gene57538.t1 MTR_6g463430 50.000 184 76 7 2 176 26 202 1.20e-44 147
MS.gene57538.t1 MTR_5g097210 50.267 187 73 7 15 185 190 372 1.68e-44 151
MS.gene57538.t1 MTR_4g116330 48.925 186 80 6 2 176 199 380 4.75e-44 150
MS.gene57538.t1 MTR_4g023190 50.267 187 70 7 2 174 180 357 1.31e-43 148
MS.gene57538.t1 MTR_4g023120 47.449 196 76 7 2 176 181 370 1.55e-43 149
MS.gene57538.t1 MTR_3g034160 50.526 190 71 9 2 175 192 374 2.16e-43 148
MS.gene57538.t1 MTR_4g117850 48.925 186 78 8 1 173 195 376 1.18e-42 146
MS.gene57538.t1 MTR_4g023150 50.000 188 70 8 2 174 180 358 5.84e-41 141
MS.gene57538.t1 MTR_4g023200 47.594 187 76 7 2 174 180 358 5.91e-40 139
MS.gene57538.t1 MTR_4g118380 53.614 166 51 9 2 149 198 355 8.58e-40 138
MS.gene57538.t1 MTR_3g034130 50.932 161 64 7 12 163 209 363 3.99e-39 137
MS.gene57538.t1 MTR_1g055175 45.026 191 79 7 2 175 19 200 3.80e-38 130
MS.gene57538.t1 MTR_5g070130 47.716 197 84 10 2 181 188 382 1.19e-37 134
MS.gene57538.t1 MTR_5g070080 47.312 186 82 9 2 176 206 386 2.48e-35 127
MS.gene57538.t1 MTR_1g055165 49.045 157 69 5 28 175 163 317 7.67e-35 125
MS.gene57538.t1 MTR_1g055195 49.398 166 70 8 27 182 323 484 5.34e-34 125
MS.gene57538.t1 MTR_4g117780 46.032 189 83 10 2 176 184 367 2.54e-33 122
MS.gene57538.t1 MTR_1g055135 49.689 161 65 7 28 175 217 374 2.68e-32 119
MS.gene57538.t1 MTR_4g118370 43.548 186 67 9 1 173 97 257 8.62e-32 115
MS.gene57538.t1 MTR_4g118400 47.222 180 64 11 1 169 134 293 5.36e-31 114
MS.gene57538.t1 MTR_4g117770 46.032 189 83 10 2 176 184 367 6.52e-31 115
MS.gene57538.t1 MTR_4g117810 46.032 189 83 10 2 176 184 367 6.52e-31 115
MS.gene57538.t1 MTR_4g117870 51.562 128 53 4 57 176 141 267 5.81e-30 110
MS.gene57538.t1 MTR_1g055250 41.206 199 96 11 1 185 87 278 1.61e-29 111
MS.gene57538.t1 MTR_4g121580 43.787 169 60 6 4 166 200 339 3.20e-29 111
MS.gene57538.t1 MTR_7g079400 42.408 191 90 8 2 176 151 337 7.66e-29 109
MS.gene57538.t1 MTR_5g070088 44.211 190 86 10 1 176 203 386 8.39e-28 107
MS.gene57538.t1 MTR_8g102520 39.572 187 97 8 2 176 140 322 1.83e-27 105
MS.gene57538.t1 MTR_4g117750 42.408 191 83 8 13 180 177 363 7.47e-26 103
MS.gene57538.t1 MTR_4g117750 51.376 109 44 4 75 174 373 481 3.81e-22 92.8
MS.gene57538.t1 MTR_4g023440 42.077 183 55 8 2 176 193 332 3.16e-25 99.8
MS.gene57538.t1 MTR_4g118360 47.656 128 52 6 60 175 1 125 7.15e-25 94.0
MS.gene57538.t1 MTR_7g079370 40.223 179 92 8 2 169 175 349 8.10e-25 99.4
MS.gene57538.t1 MTR_8g098830 42.675 157 76 8 2 149 45 196 2.09e-24 95.5
MS.gene57538.t1 MTR_4g122860 39.362 188 72 9 2 176 162 320 3.38e-24 97.4
MS.gene57538.t1 MTR_7g079420 41.899 179 82 10 12 174 971 1143 1.77e-23 97.1
MS.gene57538.t1 MTR_5g023000 55.102 98 35 4 88 176 2 99 3.16e-22 86.3
MS.gene57538.t1 MTR_4g117530 38.043 184 68 8 2 176 107 253 5.97e-22 89.7
MS.gene57538.t1 MTR_4g116330 36.022 186 65 6 2 176 199 341 3.91e-20 86.3
MS.gene57538.t1 MTR_2g048580 37.363 182 100 9 2 176 168 342 7.10e-20 85.5
MS.gene57538.t1 MTR_8g013010 54.217 83 30 3 2 84 235 309 2.08e-18 81.3
MS.gene57538.t1 MTR_4g117430 53.012 83 34 3 2 84 75 152 5.10e-18 77.0
MS.gene57538.t1 MTR_4g122840 40.310 129 46 4 51 176 164 264 1.60e-15 72.8
MS.gene57538.t1 MTR_4g023430 42.857 105 46 5 85 176 119 222 8.01e-13 64.7
MS.gene57538.t1 MTR_1g055145 54.930 71 29 3 28 98 132 199 2.23e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGTTGTCTTTGATTTAA+TGG 0.084690 8.3:-62193677 MS.gene57538:CDS
GACCATGATCCTATGTATTA+TGG 0.204606 8.3:-62193616 MS.gene57538:CDS
TATTATGGTTTGTGGGTATT+TGG 0.218240 8.3:-62193601 MS.gene57538:CDS
ATAATTGGACGGTTGAAATA+TGG 0.285135 8.3:-62193546 MS.gene57538:CDS
TTGGAGAATTTCTCAGTCTA+TGG 0.288501 8.3:-62193582 MS.gene57538:CDS
TAATCCTGCTCTAGAAGCTT+TGG 0.343636 8.3:+62193759 None:intergenic
TCTTCAATGGGGCTATTCAT+TGG 0.385937 8.3:-62193735 MS.gene57538:CDS
CAATGGGGCTATTCATTGGT+TGG 0.407106 8.3:-62193731 MS.gene57538:CDS
AATCCTGCTCTAGAAGCTTT+GGG 0.420953 8.3:+62193760 None:intergenic
AGAGCAGGATTATTCTTCAA+TGG 0.421802 8.3:-62193748 MS.gene57538:CDS
GAGCAGGATTATTCTTCAAT+GGG 0.421969 8.3:-62193747 MS.gene57538:CDS
ATGGCAACTTCAGATGCCTT+TGG 0.428120 8.3:+62193355 None:intergenic
AATTTCTCAGTCTATGGAAT+AGG 0.433887 8.3:-62193576 MS.gene57538:CDS
GGATCATGGTCGAAATCATC+TGG 0.438205 8.3:+62193628 None:intergenic
GAGTCTATGCTTTCACTCTC+CGG 0.448136 8.3:-62193325 MS.gene57538:CDS
ATCCTATGTATTATGGTTTG+TGG 0.452973 8.3:-62193609 MS.gene57538:CDS
TAATTGGACGGTTGAAATAT+GGG 0.484830 8.3:-62193545 MS.gene57538:CDS
AATACAAACAACATTCGTCT+TGG 0.508117 8.3:-62193516 MS.gene57538:CDS
GGTATTCATATAAGTGAAGC+GGG 0.509050 8.3:+62193792 None:intergenic
TCCTATGTATTATGGTTTGT+GGG 0.509991 8.3:-62193608 MS.gene57538:CDS
TATCCCAAAGCTTCTAGAGC+AGG 0.512637 8.3:-62193763 MS.gene57538:CDS
TTGTCTTTGATTTAATGGAA+AGG 0.526787 8.3:-62193672 MS.gene57538:CDS
AACCATAATACATAGGATCA+TGG 0.533187 8.3:+62193614 None:intergenic
GTAAACTTGCTCACGGTCAC+CGG 0.538352 8.3:+62193306 None:intergenic
AAAAGTGGTGATGGTGGTCA+TGG 0.546744 8.3:-62193430 MS.gene57538:CDS
AATATGTGGTATGAAATTGA+AGG 0.563922 8.3:-62193817 MS.gene57538:CDS
CTGTGGATAATGACATACCT+TGG 0.573000 8.3:-62193474 MS.gene57538:CDS
TGGTGATGGTGGTCATGGAT+TGG 0.576786 8.3:-62193425 MS.gene57538:CDS
CAAGGTATGTCATTATCCAC+AGG 0.577768 8.3:+62193475 None:intergenic
TGGTCGAAATCATCTGGAAG+AGG 0.577796 8.3:+62193634 None:intergenic
CCATCTTGTAAACTTGCTCA+CGG 0.588651 8.3:+62193299 None:intergenic
GTATCTTGAAATAGATCCAA+AGG 0.594270 8.3:-62193371 MS.gene57538:CDS
TAAATCTCTTGTAATTCCTG+TGG 0.594688 8.3:-62193491 MS.gene57538:CDS
AAGCATAGACTCTTTATACA+TGG 0.617292 8.3:+62193336 None:intergenic
TGGTATTCATATAAGTGAAG+CGG 0.623775 8.3:+62193791 None:intergenic
ACCCACAAACCATAATACAT+AGG 0.624621 8.3:+62193607 None:intergenic
AGAAGCTTTGGGATAATGCG+TGG 0.645032 8.3:+62193771 None:intergenic
CCGTGAGCAAGTTTACAAGA+TGG 0.648413 8.3:-62193299 MS.gene57538:CDS
AGCAGGATTATTCTTCAATG+GGG 0.698376 8.3:-62193746 MS.gene57538:CDS
GAGTATATAGGGGAAAACCA+AGG 0.732405 8.3:+62193457 None:intergenic
GTATTCATATAAGTGAAGCG+GGG 0.742587 8.3:+62193793 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTGTTGTCTTTGATTTAA+TGG - chr8.3:62193416-62193435 MS.gene57538:CDS 20.0%
!! ACTTTTTGTAGAGTATATAG+GGG + chr8.3:62193649-62193668 None:intergenic 25.0%
!! TTGTCTTTGATTTAATGGAA+AGG - chr8.3:62193421-62193440 MS.gene57538:CDS 25.0%
!!! CACTTTTTGTAGAGTATATA+GGG + chr8.3:62193650-62193669 None:intergenic 25.0%
!!! GGAATAGGAATTTTGATAAT+TGG - chr8.3:62193532-62193551 MS.gene57538:CDS 25.0%
!!! TAGGAATTTTGATAATTGGA+CGG - chr8.3:62193536-62193555 MS.gene57538:CDS 25.0%
AACCATAATACATAGGATCA+TGG + chr8.3:62193482-62193501 None:intergenic 30.0%
AAGCATAGACTCTTTATACA+TGG + chr8.3:62193760-62193779 None:intergenic 30.0%
AATACAAACAACATTCGTCT+TGG - chr8.3:62193577-62193596 MS.gene57538:CDS 30.0%
AATTTCTCAGTCTATGGAAT+AGG - chr8.3:62193517-62193536 MS.gene57538:CDS 30.0%
ATAATTGGACGGTTGAAATA+TGG - chr8.3:62193547-62193566 MS.gene57538:CDS 30.0%
ATCCTATGTATTATGGTTTG+TGG - chr8.3:62193484-62193503 MS.gene57538:CDS 30.0%
CCTATATACTCTACAAAAAG+TGG - chr8.3:62193648-62193667 MS.gene57538:CDS 30.0%
GTATCTTGAAATAGATCCAA+AGG - chr8.3:62193722-62193741 MS.gene57538:CDS 30.0%
TAAATCTCTTGTAATTCCTG+TGG - chr8.3:62193602-62193621 MS.gene57538:CDS 30.0%
TAATTGGACGGTTGAAATAT+GGG - chr8.3:62193548-62193567 MS.gene57538:CDS 30.0%
TGGTATTCATATAAGTGAAG+CGG + chr8.3:62193305-62193324 None:intergenic 30.0%
! TATTATGGTTTGTGGGTATT+TGG - chr8.3:62193492-62193511 MS.gene57538:CDS 30.0%
! TCCTATGTATTATGGTTTGT+GGG - chr8.3:62193485-62193504 MS.gene57538:CDS 30.0%
!!! CCACTTTTTGTAGAGTATAT+AGG + chr8.3:62193651-62193670 None:intergenic 30.0%
ACCCACAAACCATAATACAT+AGG + chr8.3:62193489-62193508 None:intergenic 35.0%
AGAGCAGGATTATTCTTCAA+TGG - chr8.3:62193345-62193364 MS.gene57538:CDS 35.0%
AGCAGGATTATTCTTCAATG+GGG - chr8.3:62193347-62193366 MS.gene57538:CDS 35.0%
GACCATGATCCTATGTATTA+TGG - chr8.3:62193477-62193496 MS.gene57538:CDS 35.0%
GAGCAGGATTATTCTTCAAT+GGG - chr8.3:62193346-62193365 MS.gene57538:CDS 35.0%
GGTATTCATATAAGTGAAGC+GGG + chr8.3:62193304-62193323 None:intergenic 35.0%
GTATTCATATAAGTGAAGCG+GGG + chr8.3:62193303-62193322 None:intergenic 35.0%
TACTCTACAAAAAGTGGTGA+TGG - chr8.3:62193654-62193673 MS.gene57538:CDS 35.0%
TTGGAGAATTTCTCAGTCTA+TGG - chr8.3:62193511-62193530 MS.gene57538:CDS 35.0%
CAAGGTATGTCATTATCCAC+AGG + chr8.3:62193621-62193640 None:intergenic 40.0%
CTGTGGATAATGACATACCT+TGG - chr8.3:62193619-62193638 MS.gene57538:CDS 40.0%
GAGTATATAGGGGAAAACCA+AGG + chr8.3:62193639-62193658 None:intergenic 40.0%
TCTTCAATGGGGCTATTCAT+TGG - chr8.3:62193358-62193377 MS.gene57538:CDS 40.0%
! CCATCTTGTAAACTTGCTCA+CGG + chr8.3:62193797-62193816 None:intergenic 40.0%
!! AATCCTGCTCTAGAAGCTTT+GGG + chr8.3:62193336-62193355 None:intergenic 40.0%
!! TAATCCTGCTCTAGAAGCTT+TGG + chr8.3:62193337-62193356 None:intergenic 40.0%
!! TCTACAAAAAGTGGTGATGG+TGG - chr8.3:62193657-62193676 MS.gene57538:CDS 40.0%
ATGGCAACTTCAGATGCCTT+TGG + chr8.3:62193741-62193760 None:intergenic 45.0%
CAATGGGGCTATTCATTGGT+TGG - chr8.3:62193362-62193381 MS.gene57538:CDS 45.0%
CCGTGAGCAAGTTTACAAGA+TGG - chr8.3:62193794-62193813 MS.gene57538:CDS 45.0%
GAGTCTATGCTTTCACTCTC+CGG - chr8.3:62193768-62193787 MS.gene57538:CDS 45.0%
GGATCATGGTCGAAATCATC+TGG + chr8.3:62193468-62193487 None:intergenic 45.0%
TGGTCGAAATCATCTGGAAG+AGG + chr8.3:62193462-62193481 None:intergenic 45.0%
!! AAAAGTGGTGATGGTGGTCA+TGG - chr8.3:62193663-62193682 MS.gene57538:CDS 45.0%
!! AGAAGCTTTGGGATAATGCG+TGG + chr8.3:62193325-62193344 None:intergenic 45.0%
!! TATCCCAAAGCTTCTAGAGC+AGG - chr8.3:62193330-62193349 MS.gene57538:CDS 45.0%
! GTAAACTTGCTCACGGTCAC+CGG + chr8.3:62193790-62193809 None:intergenic 50.0%
!! TGGTGATGGTGGTCATGGAT+TGG - chr8.3:62193668-62193687 MS.gene57538:CDS 50.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 62193279 62193836 62193279 ID=MS.gene57538
chr8.3 mRNA 62193279 62193836 62193279 ID=MS.gene57538.t1;Parent=MS.gene57538
chr8.3 exon 62193279 62193836 62193279 ID=MS.gene57538.t1.exon1;Parent=MS.gene57538.t1
chr8.3 CDS 62193279 62193836 62193279 ID=cds.MS.gene57538.t1;Parent=MS.gene57538.t1
Gene Sequence

>MS.gene57538

ATGTGGTATGAAATTGAAGGTCCCCGCTTCACTTATATGAATACCACGCATTATCCCAAAGCTTCTAGAGCAGGATTATTCTTCAATGGGGCTATTCATTGGTTGGCTTCTCGTTATCATGATATGCGAGTGAAAGTTATTGTTGTCTTTGATTTAATGGAAAGGAAACTGTTAGAGATACCTCTTCCAGATGATTTCGACCATGATCCTATGTATTATGGTTTGTGGGTATTTGGAGAATTTCTCAGTCTATGGAATAGGAATTTTGATAATTGGACGGTTGAAATATGGGTGATGAAATACAAACAACATTCGTCTTGGACTAAATCTCTTGTAATTCCTGTGGATAATGACATACCTTGGTTTTCCCCTATATACTCTACAAAAAGTGGTGATGGTGGTCATGGATTGGTGAAGTATAATAACAAAAGTCAACTGTTAGAGTATCTTGAAATAGATCCAAAGGCATCTGAAGTTGCCATGTATAAAGAGTCTATGCTTTCACTCTCCGGTGACCGTGAGCAAGTTTACAAGATGGCAAAAAATCACGAAGAATGA

Protein sequence

>MS.gene57538.t1

MWYEIEGPRFTYMNTTHYPKASRAGLFFNGAIHWLASRYHDMRVKVIVVFDLMERKLLEIPLPDDFDHDPMYYGLWVFGEFLSLWNRNFDNWTVEIWVMKYKQHSSWTKSLVIPVDNDIPWFSPIYSTKSGDGGHGLVKYNNKSQLLEYLEIDPKASEVAMYKESMLSLSGDREQVYKMAKNHEE