AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033079


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033079.t1 MTR_4g109270 61.458 192 68 4 10 195 173 364 2.57e-70 218
MS.gene033079.t1 MTR_3g103020 56.186 194 76 5 10 195 186 378 1.89e-62 198
MS.gene033079.t1 MTR_6g060650 55.155 194 83 4 4 194 180 372 9.78e-61 193
MS.gene033079.t1 MTR_4g117720 54.592 196 78 6 10 195 184 378 1.47e-58 188
MS.gene033079.t1 MTR_4g109280 53.403 191 83 4 10 195 170 359 1.07e-57 185
MS.gene033079.t1 MTR_8g040935 50.739 203 86 6 4 195 9 208 2.44e-56 177
MS.gene033079.t1 MTR_3g034180 53.093 194 81 5 10 194 296 488 4.10e-56 184
MS.gene033079.t1 MTR_4g116330 52.041 196 80 5 10 195 189 380 4.98e-56 181
MS.gene033079.t1 MTR_3g034160 51.813 193 85 3 10 194 182 374 9.45e-54 176
MS.gene033079.t1 MTR_4g117850 54.167 192 78 5 10 192 186 376 1.45e-53 175
MS.gene033079.t1 MTR_4g122850 50.777 193 88 5 10 195 172 364 4.84e-53 174
MS.gene033079.t1 MTR_3g034130 52.486 181 77 4 10 181 182 362 1.05e-52 172
MS.gene033079.t1 MTR_4g116320 50.000 192 82 5 10 192 69 255 1.62e-51 166
MS.gene033079.t1 MTR_4g023130 53.158 190 80 6 10 194 127 312 3.36e-51 167
MS.gene033079.t1 MTR_4g023080 52.128 188 83 5 10 193 168 352 6.93e-51 167
MS.gene033079.t1 MTR_8g433030 50.794 189 87 4 7 190 150 337 2.26e-50 166
MS.gene033079.t1 MTR_8g032390 51.613 186 84 4 10 190 186 370 7.13e-50 166
MS.gene033079.t1 MTR_4g021785 50.526 190 88 4 10 195 168 355 1.64e-49 164
MS.gene033079.t1 MTR_6g463430 49.231 195 82 5 10 195 16 202 3.57e-49 159
MS.gene033079.t1 MTR_8g433040 49.738 191 80 4 10 195 185 364 1.28e-48 162
MS.gene033079.t1 MTR_8g032380 49.738 191 80 4 10 195 185 364 1.28e-48 162
MS.gene033079.t1 MTR_4g117780 48.969 194 91 4 10 195 174 367 1.54e-48 162
MS.gene033079.t1 MTR_4g117770 48.969 194 91 4 10 195 174 367 6.52e-48 160
MS.gene033079.t1 MTR_4g117810 48.969 194 91 4 10 195 174 367 6.52e-48 160
MS.gene033079.t1 MTR_4g023090 47.317 205 85 7 10 194 184 385 1.77e-47 159
MS.gene033079.t1 MTR_4g094365 47.396 192 94 4 10 195 174 364 1.13e-46 157
MS.gene033079.t1 MTR_4g023380 46.262 214 84 7 10 195 169 379 1.19e-45 155
MS.gene033079.t1 MTR_4g023200 49.215 191 86 6 11 193 171 358 2.56e-45 153
MS.gene033079.t1 MTR_4g118380 50.877 171 72 4 10 169 188 357 6.06e-45 152
MS.gene033079.t1 MTR_4g023070 45.498 211 95 6 1 194 162 369 7.31e-45 152
MS.gene033079.t1 MTR_4g023120 44.828 203 92 5 10 195 171 370 2.33e-44 151
MS.gene033079.t1 MTR_4g023190 48.438 192 87 6 10 193 170 357 6.26e-44 149
MS.gene033079.t1 MTR_1g055175 44.041 193 101 4 10 195 9 201 4.65e-43 143
MS.gene033079.t1 MTR_4g023150 48.438 192 88 6 10 193 170 358 2.15e-42 145
MS.gene033079.t1 MTR_5g070080 46.354 192 96 5 10 195 196 386 4.15e-42 145
MS.gene033079.t1 MTR_4g118400 47.120 191 87 5 1 189 116 294 4.49e-42 143
MS.gene033079.t1 MTR_5g097210 47.594 187 92 5 10 191 174 359 7.95e-42 144
MS.gene033079.t1 MTR_5g070130 45.050 202 93 9 10 195 178 377 4.61e-41 143
MS.gene033079.t1 MTR_1g055195 43.689 206 104 7 1 195 274 478 8.67e-41 144
MS.gene033079.t1 MTR_4g117530 42.347 196 82 4 1 195 88 253 1.04e-39 136
MS.gene033079.t1 MTR_4g117870 43.386 189 69 4 10 195 114 267 1.65e-39 136
MS.gene033079.t1 MTR_4g118370 41.270 189 86 6 10 192 88 257 8.77e-37 129
MS.gene033079.t1 MTR_1g055135 42.929 198 95 7 12 195 182 375 2.07e-36 130
MS.gene033079.t1 MTR_5g070088 43.750 192 98 5 10 193 194 383 6.00e-36 129
MS.gene033079.t1 MTR_1g055165 44.444 162 86 2 38 195 157 318 1.00e-35 127
MS.gene033079.t1 MTR_4g117750 41.919 198 100 6 10 194 163 358 6.93e-35 128
MS.gene033079.t1 MTR_4g117750 55.963 109 43 3 90 193 373 481 1.57e-26 105
MS.gene033079.t1 MTR_4g116330 42.487 193 64 7 10 195 189 341 1.65e-34 125
MS.gene033079.t1 MTR_7g079400 41.919 198 102 7 10 195 141 337 2.92e-34 124
MS.gene033079.t1 MTR_4g023440 41.711 187 71 4 10 195 183 332 6.93e-34 123
MS.gene033079.t1 MTR_8g102520 38.144 194 111 5 10 195 130 322 5.23e-32 117
MS.gene033079.t1 MTR_5g023000 62.366 93 33 2 105 195 7 99 1.47e-31 110
MS.gene033079.t1 MTR_1g055250 40.782 179 97 6 10 182 78 253 1.66e-31 116
MS.gene033079.t1 MTR_4g118360 53.043 115 49 3 85 195 13 126 1.02e-30 109
MS.gene033079.t1 MTR_7g079370 41.237 194 92 8 10 189 165 350 3.20e-30 114
MS.gene033079.t1 MTR_2g048580 37.306 193 106 7 10 195 158 342 4.67e-27 105
MS.gene033079.t1 MTR_4g117430 52.174 92 42 1 10 101 65 154 2.18e-24 94.0
MS.gene033079.t1 MTR_8g098830 38.788 165 87 6 10 165 35 194 3.20e-24 95.5
MS.gene033079.t1 MTR_4g122860 36.126 191 85 8 15 195 157 320 4.95e-23 94.4
MS.gene033079.t1 MTR_7g079450 41.549 142 71 5 60 195 2 137 1.11e-22 89.0
MS.gene033079.t1 MTR_7g079420 36.667 180 100 7 27 195 968 1144 1.79e-21 91.7
MS.gene033079.t1 MTR_8g013010 51.579 95 31 3 10 99 225 309 2.57e-21 89.4
MS.gene033079.t1 MTR_4g114700 33.333 183 71 5 10 186 81 218 4.51e-19 82.0
MS.gene033079.t1 MTR_6g034720 56.716 67 28 1 10 76 104 169 8.31e-18 78.2
MS.gene033079.t1 MTR_4g121580 36.646 161 67 7 10 163 186 318 1.70e-17 79.7
MS.gene033079.t1 MTR_8g013005 55.932 59 25 1 138 195 1 59 9.25e-15 66.2
MS.gene033079.t1 MTR_4g023430 50.617 81 35 3 120 195 142 222 4.52e-14 68.2
MS.gene033079.t1 MTR_7g117720 50.575 87 37 3 110 190 186 272 9.34e-13 65.5
MS.gene033079.t1 MTR_4g109290 64.151 53 18 1 144 195 136 188 2.08e-12 63.2
MS.gene033079.t1 MTR_1g094210 26.842 190 114 7 11 187 221 398 7.13e-11 60.8
MS.gene033079.t1 MTR_4g116340 50.000 56 27 1 10 65 193 247 7.95e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033079.t1 AT3G06240 28.261 184 118 4 12 187 242 419 6.26e-12 63.9

Find 47 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGCTGGGTTGTTCTTTAA+TGG 0.210542 8.3:+15331963 MS.gene033079:CDS
GATTCTGGTTTGTGGGTATT+TGG 0.239819 8.3:+15332107 MS.gene033079:CDS
AAACATGAACCTTACGATTC+TGG 0.242156 8.3:+15332092 MS.gene033079:CDS
TGGGTATTTGGAGAATTTCT+TGG 0.272087 8.3:+15332119 MS.gene033079:CDS
TTATACTTCACCAATCTATT+AGG 0.281531 8.3:-15332302 None:intergenic
GGTTCATGTTTAAGCTTATA+TGG 0.295734 8.3:-15332080 None:intergenic
AAGCTGGGTTGTTCTTTAAT+GGG 0.303358 8.3:+15331964 MS.gene033079:CDS
GAGTCTCTGCTTTCACTTCC+CGG 0.311578 8.3:+15332395 MS.gene033079:CDS
ATCGTGGTGCAGCTGAAATA+TGG 0.311591 8.3:+15332153 MS.gene033079:CDS
TAATACATGGAAACAAATTC+AGG 0.328938 8.3:+15331899 MS.gene033079:CDS
AGGTCCATATGATTCGTTAT+AGG 0.333106 8.3:-15332354 None:intergenic
TTGGAGAATTTCTTGGTTTG+TGG 0.336914 8.3:+15332126 MS.gene033079:CDS
TCTTTAATGGGGCTATTCAT+TGG 0.349624 8.3:+15331976 MS.gene033079:CDS
TGGAGAATTTCTTGGTTTGT+GGG 0.354642 8.3:+15332127 MS.gene033079:CDS
ATTAAAGAACAACCCAGCTT+TGG 0.366565 8.3:-15331961 None:intergenic
TCTGTATACACGGCCACTTC+AGG 0.389246 8.3:-15332374 None:intergenic
TTAAAGAACAACCCAGCTTT+GGG 0.389953 8.3:-15331960 None:intergenic
ACCTTACGATTCTGGTTTGT+GGG 0.398636 8.3:+15332100 MS.gene033079:CDS
TGCAGAGCATAAAGGAGAAA+AGG 0.417056 8.3:-15332249 None:intergenic
TAATGGGGCTATTCATTGGT+TGG 0.427301 8.3:+15331980 MS.gene033079:CDS
TCTGAACATGAGCCCAAAGC+TGG 0.430995 8.3:+15331948 MS.gene033079:CDS
AACCTTACGATTCTGGTTTG+TGG 0.435471 8.3:+15332099 MS.gene033079:CDS
TCATCTTCATACATGTCTCT+TGG 0.436970 8.3:+15331857 MS.gene033079:CDS
CGCTCCTATAACGAATCATA+TGG 0.447526 8.3:+15332350 MS.gene033079:CDS
TTAAGCTTGTTGATTGTCAC+CGG 0.464950 8.3:-15332414 None:intergenic
GCAGAGCATAAAGGAGAAAA+GGG 0.474610 8.3:-15332248 None:intergenic
TTTCCTTTCAATTAAATCAA+AGG 0.474723 8.3:-15332039 None:intergenic
AACAGATGATCCTAATAGAT+TGG 0.485016 8.3:+15332292 MS.gene033079:CDS
TGTTTAAGCTTATATGGCAC+AGG 0.490002 8.3:-15332074 None:intergenic
TATACAAAGTGCATAAGTCT+TGG 0.491874 8.3:+15332186 MS.gene033079:CDS
GGGTGTTGAATGCCATCAAA+AGG 0.508697 8.3:-15332227 None:intergenic
TTGCCTTTGATTTAATTGAA+AGG 0.515684 8.3:+15332036 MS.gene033079:CDS
TCTCATTGAGAACTAATACA+TGG 0.535970 8.3:+15331886 MS.gene033079:CDS
AGCTGGGTTGTTCTTTAATG+GGG 0.543964 8.3:+15331965 MS.gene033079:CDS
TCGTGGTGCAGCTGAAATAT+GGG 0.550093 8.3:+15332154 MS.gene033079:CDS
AATACATGGAAACAAATTCA+GGG 0.551639 8.3:+15331900 MS.gene033079:CDS
AGATTGGTGAAGTATAATCA+TGG 0.553410 8.3:+15332308 MS.gene033079:CDS
CGAATCATATGGACCTGAAG+TGG 0.577896 8.3:+15332361 MS.gene033079:CDS
CTGAACATGAGCCCAAAGCT+GGG 0.581867 8.3:+15331949 MS.gene033079:CDS
ACCCACAAACCAGAATCGTA+AGG 0.614833 8.3:-15332101 None:intergenic
GAGACATGTATGAAGATGAA+CGG 0.643604 8.3:-15331853 None:intergenic
AAGCAGAGACTCTGTATACA+CGG 0.652046 8.3:-15332384 None:intergenic
TAAGCTTGTTGATTGTCACC+GGG 0.663785 8.3:-15332413 None:intergenic
TTGGTGAAGTATAATCATGG+AGG 0.690121 8.3:+15332311 MS.gene033079:CDS
CTTGGTTTGTGGGCTCATCG+TGG 0.695842 8.3:+15332137 MS.gene033079:CDS
GCAGATGCATACATGCAATG+AGG 0.696847 8.3:-15331924 None:intergenic
CAGAGCATAAAGGAGAAAAG+GGG 0.698195 8.3:-15332247 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCCTTTCAATTAAATCAA+AGG - chr8.3:15332042-15332061 None:intergenic 20.0%
!!! GCAAAAAAAGGTATTATTAT+TGG + chr8.3:15332269-15332288 MS.gene033079:CDS 20.0%
! AATACATGGAAACAAATTCA+GGG + chr8.3:15331900-15331919 MS.gene033079:CDS 25.0%
! TAATACATGGAAACAAATTC+AGG + chr8.3:15331899-15331918 MS.gene033079:CDS 25.0%
! TTATACTTCACCAATCTATT+AGG - chr8.3:15332305-15332324 None:intergenic 25.0%
!! TTGCCTTTGATTTAATTGAA+AGG + chr8.3:15332036-15332055 MS.gene033079:CDS 25.0%
AACAGATGATCCTAATAGAT+TGG + chr8.3:15332292-15332311 MS.gene033079:CDS 30.0%
AGATTGGTGAAGTATAATCA+TGG + chr8.3:15332308-15332327 MS.gene033079:CDS 30.0%
GGTTCATGTTTAAGCTTATA+TGG - chr8.3:15332083-15332102 None:intergenic 30.0%
TATACAAAGTGCATAAGTCT+TGG + chr8.3:15332186-15332205 MS.gene033079:CDS 30.0%
TCTCATTGAGAACTAATACA+TGG + chr8.3:15331886-15331905 MS.gene033079:CDS 30.0%
! ACTCATGTTTTTCCTTTTGA+TGG + chr8.3:15332215-15332234 MS.gene033079:CDS 30.0%
!!! TTTTCGTCTTGATTTGCTAA+AGG + chr8.3:15332004-15332023 MS.gene033079:CDS 30.0%
AAACATGAACCTTACGATTC+TGG + chr8.3:15332092-15332111 MS.gene033079:CDS 35.0%
AAAGCTGGGTTGTTCTTTAA+TGG + chr8.3:15331963-15331982 MS.gene033079:CDS 35.0%
AAGCTGGGTTGTTCTTTAAT+GGG + chr8.3:15331964-15331983 MS.gene033079:CDS 35.0%
AGGTCCATATGATTCGTTAT+AGG - chr8.3:15332357-15332376 None:intergenic 35.0%
ATTAAAGAACAACCCAGCTT+TGG - chr8.3:15331964-15331983 None:intergenic 35.0%
CCTTTATGCTCTGCAAAAAA+AGG + chr8.3:15332257-15332276 MS.gene033079:CDS 35.0%
GAGACATGTATGAAGATGAA+CGG - chr8.3:15331856-15331875 None:intergenic 35.0%
TCATCTTCATACATGTCTCT+TGG + chr8.3:15331857-15331876 MS.gene033079:CDS 35.0%
TCTTTAATGGGGCTATTCAT+TGG + chr8.3:15331976-15331995 MS.gene033079:CDS 35.0%
TGGGTATTTGGAGAATTTCT+TGG + chr8.3:15332119-15332138 MS.gene033079:CDS 35.0%
TGTTTAAGCTTATATGGCAC+AGG - chr8.3:15332077-15332096 None:intergenic 35.0%
TTAAAGAACAACCCAGCTTT+GGG - chr8.3:15331963-15331982 None:intergenic 35.0%
TTGGTGAAGTATAATCATGG+AGG + chr8.3:15332311-15332330 MS.gene033079:CDS 35.0%
! TTGGAGAATTTCTTGGTTTG+TGG + chr8.3:15332126-15332145 MS.gene033079:CDS 35.0%
!! TGGAGAATTTCTTGGTTTGT+GGG + chr8.3:15332127-15332146 MS.gene033079:CDS 35.0%
!! TTAAGCTTGTTGATTGTCAC+CGG - chr8.3:15332417-15332436 None:intergenic 35.0%
!!! CCTTTTTTTGCAGAGCATAA+AGG - chr8.3:15332260-15332279 None:intergenic 35.0%
AAGCAGAGACTCTGTATACA+CGG - chr8.3:15332387-15332406 None:intergenic 40.0%
AGCTGGGTTGTTCTTTAATG+GGG + chr8.3:15331965-15331984 MS.gene033079:CDS 40.0%
CAGAGCATAAAGGAGAAAAG+GGG - chr8.3:15332250-15332269 None:intergenic 40.0%
CGCTCCTATAACGAATCATA+TGG + chr8.3:15332350-15332369 MS.gene033079:CDS 40.0%
GCAGAGCATAAAGGAGAAAA+GGG - chr8.3:15332251-15332270 None:intergenic 40.0%
TAATGGGGCTATTCATTGGT+TGG + chr8.3:15331980-15331999 MS.gene033079:CDS 40.0%
TGCAGAGCATAAAGGAGAAA+AGG - chr8.3:15332252-15332271 None:intergenic 40.0%
!! AACCTTACGATTCTGGTTTG+TGG + chr8.3:15332099-15332118 MS.gene033079:CDS 40.0%
!! ACCTTACGATTCTGGTTTGT+GGG + chr8.3:15332100-15332119 MS.gene033079:CDS 40.0%
!! GATTCTGGTTTGTGGGTATT+TGG + chr8.3:15332107-15332126 MS.gene033079:CDS 40.0%
!! TAAGCTTGTTGATTGTCACC+GGG - chr8.3:15332416-15332435 None:intergenic 40.0%
ACCCACAAACCAGAATCGTA+AGG - chr8.3:15332104-15332123 None:intergenic 45.0%
ATCGTGGTGCAGCTGAAATA+TGG + chr8.3:15332153-15332172 MS.gene033079:CDS 45.0%
CGAATCATATGGACCTGAAG+TGG + chr8.3:15332361-15332380 MS.gene033079:CDS 45.0%
GCAGATGCATACATGCAATG+AGG - chr8.3:15331927-15331946 None:intergenic 45.0%
TCGTGGTGCAGCTGAAATAT+GGG + chr8.3:15332154-15332173 MS.gene033079:CDS 45.0%
!! GGGTGTTGAATGCCATCAAA+AGG - chr8.3:15332230-15332249 None:intergenic 45.0%
CTGAACATGAGCCCAAAGCT+GGG + chr8.3:15331949-15331968 MS.gene033079:CDS 50.0%
GAGTCTCTGCTTTCACTTCC+CGG + chr8.3:15332395-15332414 MS.gene033079:CDS 50.0%
TCTGAACATGAGCCCAAAGC+TGG + chr8.3:15331948-15331967 MS.gene033079:CDS 50.0%
TCTGTATACACGGCCACTTC+AGG - chr8.3:15332377-15332396 None:intergenic 50.0%
!! CTTGGTTTGTGGGCTCATCG+TGG + chr8.3:15332137-15332156 MS.gene033079:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 15331849 15332436 15331849 ID=MS.gene033079
chr8.3 mRNA 15331849 15332436 15331849 ID=MS.gene033079.t1;Parent=MS.gene033079
chr8.3 exon 15331849 15332436 15331849 ID=MS.gene033079.t1.exon1;Parent=MS.gene033079.t1
chr8.3 CDS 15331849 15332436 15331849 ID=cds.MS.gene033079.t1;Parent=MS.gene033079.t1
Gene Sequence

>MS.gene033079

ATGTCCGTTCATCTTCATACATGTCTCTTGGAGATTTTCTCATTGAGAACTAATACATGGAAACAAATTCAGGGTCCTCATTGCATGTATGCATCTGCTTCTGAACATGAGCCCAAAGCTGGGTTGTTCTTTAATGGGGCTATTCATTGGTTGGCTTTTCGTCTTGATTTGCTAAAGGATGTTATTGTTGCCTTTGATTTAATTGAAAGGAAATTTTTTTGTATGCCTGTGCCATATAAGCTTAAACATGAACCTTACGATTCTGGTTTGTGGGTATTTGGAGAATTTCTTGGTTTGTGGGCTCATCGTGGTGCAGCTGAAATATGGGTGATGAAAGTATACAAAGTGCATAAGTCTTGGACAAACACTCATGTTTTTCCTTTTGATGGCATTCAACACCCCTTTTCTCCTTTATGCTCTGCAAAAAAAGGTATTATTATTGGAACAGATGATCCTAATAGATTGGTGAAGTATAATCATGGAGGAAAGTTGTTAGAGTGTCGCTCCTATAACGAATCATATGGACCTGAAGTGGCCGTGTATACAGAGTCTCTGCTTTCACTTCCCGGTGACAATCAACAAGCTTAA

Protein sequence

>MS.gene033079.t1

MSVHLHTCLLEIFSLRTNTWKQIQGPHCMYASASEHEPKAGLFFNGAIHWLAFRLDLLKDVIVAFDLIERKFFCMPVPYKLKHEPYDSGLWVFGEFLGLWAHRGAAEIWVMKVYKVHKSWTNTHVFPFDGIQHPFSPLCSAKKGIIIGTDDPNRLVKYNHGGKLLECRSYNESYGPEVAVYTESLLSLPGDNQQA