AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28758


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28758.t1 MTR_4g094365 79.104 134 26 1 1 132 231 364 2.37e-69 213
MS.gene28758.t1 MTR_8g433040 61.364 132 49 1 1 132 235 364 1.39e-47 157
MS.gene28758.t1 MTR_8g032380 61.364 132 49 1 1 132 235 364 1.39e-47 157
MS.gene28758.t1 MTR_8g433030 62.992 127 44 2 1 126 212 336 4.90e-46 152
MS.gene28758.t1 MTR_8g032390 62.992 127 44 2 1 126 245 369 1.26e-45 152
MS.gene28758.t1 MTR_4g122850 57.463 134 54 3 1 132 232 364 8.49e-45 150
MS.gene28758.t1 MTR_4g116330 56.296 135 56 2 1 132 246 380 5.73e-44 147
MS.gene28758.t1 MTR_6g060650 56.061 132 55 2 1 131 243 372 1.82e-42 144
MS.gene28758.t1 MTR_8g040935 54.478 134 58 2 2 132 75 208 1.06e-41 137
MS.gene28758.t1 MTR_4g109270 56.391 133 57 1 1 132 232 364 2.15e-41 140
MS.gene28758.t1 MTR_3g103020 54.887 133 59 1 1 132 246 378 3.70e-41 140
MS.gene28758.t1 MTR_4g116320 55.725 131 55 2 1 129 126 255 4.27e-41 137
MS.gene28758.t1 MTR_4g109280 57.037 135 52 4 1 132 228 359 5.58e-41 139
MS.gene28758.t1 MTR_4g117870 51.562 128 61 1 5 132 141 267 6.66e-36 124
MS.gene28758.t1 MTR_4g117780 57.143 112 47 1 22 132 256 367 2.03e-35 125
MS.gene28758.t1 MTR_4g117770 57.143 112 47 1 22 132 256 367 2.48e-35 125
MS.gene28758.t1 MTR_4g117810 57.143 112 47 1 22 132 256 367 2.48e-35 125
MS.gene28758.t1 MTR_4g117720 51.515 132 56 4 1 126 242 371 3.28e-35 125
MS.gene28758.t1 MTR_4g023130 53.383 133 56 4 1 131 184 312 4.01e-35 123
MS.gene28758.t1 MTR_3g034180 52.632 133 59 3 1 132 360 489 4.20e-35 126
MS.gene28758.t1 MTR_4g117850 53.968 126 56 2 2 125 248 373 4.68e-35 124
MS.gene28758.t1 MTR_4g023080 51.880 133 58 4 1 131 225 353 1.05e-33 120
MS.gene28758.t1 MTR_4g118360 50.781 128 56 5 8 131 1 125 1.61e-33 114
MS.gene28758.t1 MTR_4g122860 52.344 128 53 3 10 132 196 320 1.79e-33 119
MS.gene28758.t1 MTR_5g097210 52.344 128 59 1 2 129 235 360 2.65e-33 120
MS.gene28758.t1 MTR_1g055175 47.727 132 67 2 1 131 70 200 2.78e-33 115
MS.gene28758.t1 MTR_4g023380 45.223 157 59 5 1 132 225 379 3.53e-33 119
MS.gene28758.t1 MTR_4g118370 50.769 130 59 3 1 129 132 257 1.40e-32 115
MS.gene28758.t1 MTR_4g023090 46.479 142 57 5 1 126 240 378 2.21e-32 117
MS.gene28758.t1 MTR_5g023000 55.556 99 43 1 35 132 1 99 2.47e-32 110
MS.gene28758.t1 MTR_4g116330 55.856 111 46 2 25 132 231 341 5.45e-32 115
MS.gene28758.t1 MTR_1g055165 47.727 132 67 2 1 131 187 317 6.90e-32 115
MS.gene28758.t1 MTR_4g117750 55.556 108 47 1 24 130 374 481 3.20e-31 115
MS.gene28758.t1 MTR_4g117750 46.281 121 60 3 12 131 242 358 1.27e-24 97.8
MS.gene28758.t1 MTR_4g021785 48.529 136 60 4 1 132 226 355 3.93e-31 114
MS.gene28758.t1 MTR_6g463430 50.000 134 60 4 1 132 74 202 4.08e-31 110
MS.gene28758.t1 MTR_1g055195 50.781 128 58 3 1 126 348 472 7.01e-31 115
MS.gene28758.t1 MTR_4g023070 45.833 144 62 4 2 131 228 369 1.77e-30 112
MS.gene28758.t1 MTR_3g034160 53.030 132 58 3 1 131 246 374 8.09e-30 110
MS.gene28758.t1 MTR_4g023120 44.595 148 60 4 2 132 228 370 2.18e-29 109
MS.gene28758.t1 MTR_1g055135 47.794 136 64 4 1 131 241 374 2.22e-29 109
MS.gene28758.t1 MTR_5g070080 48.413 126 63 2 2 126 255 379 5.24e-29 108
MS.gene28758.t1 MTR_3g034130 51.351 111 51 2 1 110 246 354 5.46e-29 108
MS.gene28758.t1 MTR_5g070130 46.875 128 66 2 1 126 243 370 1.64e-28 107
MS.gene28758.t1 MTR_4g023190 45.985 137 63 6 1 130 225 357 5.98e-28 105
MS.gene28758.t1 MTR_4g023200 45.985 137 63 6 1 130 226 358 1.34e-27 104
MS.gene28758.t1 MTR_4g118380 47.826 115 48 3 1 105 245 357 7.25e-27 102
MS.gene28758.t1 MTR_7g079450 46.212 132 65 4 2 132 11 137 1.09e-26 97.1
MS.gene28758.t1 MTR_4g023150 45.255 137 64 6 1 130 226 358 1.62e-26 101
MS.gene28758.t1 MTR_4g117530 51.376 109 47 3 25 132 150 253 1.75e-26 99.8
MS.gene28758.t1 MTR_8g102520 46.269 134 66 4 2 132 192 322 8.71e-26 99.4
MS.gene28758.t1 MTR_4g118400 45.238 126 54 3 2 126 183 294 1.57e-25 98.2
MS.gene28758.t1 MTR_5g070088 43.750 128 70 2 1 126 252 379 2.33e-24 96.3
MS.gene28758.t1 MTR_4g023440 56.383 94 36 3 41 132 242 332 2.86e-24 95.5
MS.gene28758.t1 MTR_1g055250 46.667 105 54 2 2 106 140 242 2.10e-23 92.8
MS.gene28758.t1 MTR_7g079400 43.478 138 68 5 2 132 203 337 9.55e-22 88.6
MS.gene28758.t1 MTR_4g114700 54.023 87 36 2 41 123 132 218 3.92e-20 82.8
MS.gene28758.t1 MTR_7g117720 55.814 86 33 3 46 126 186 271 6.85e-19 80.1
MS.gene28758.t1 MTR_4g122840 38.931 131 45 3 3 132 168 264 1.04e-18 79.3
MS.gene28758.t1 MTR_4g121580 41.221 131 62 5 4 131 230 348 1.08e-18 80.9
MS.gene28758.t1 MTR_8g013005 61.017 59 23 0 74 132 1 59 7.48e-17 69.7
MS.gene28758.t1 MTR_4g023430 53.659 82 32 3 56 132 142 222 2.00e-16 72.8
MS.gene28758.t1 MTR_7g079420 39.231 130 70 5 2 126 1013 1138 4.41e-16 73.9
MS.gene28758.t1 MTR_7g079370 41.085 129 67 5 2 126 227 350 4.63e-16 73.2
MS.gene28758.t1 MTR_8g098830 43.396 106 54 4 2 105 97 198 5.41e-16 71.6
MS.gene28758.t1 MTR_4g023100 56.140 57 23 1 76 132 65 119 1.30e-13 63.2
MS.gene28758.t1 MTR_4g109290 62.264 53 20 0 80 132 136 188 4.93e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 27 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTTGGTTCGTGGGTATT+TGG 0.245938 4.3:+19842716 MS.gene28758:CDS
ATAGCCGTACACTTAAAATA+TGG 0.266688 4.3:+19842771 MS.gene28758:CDS
TAAACTTGCTCTCTATCAAC+AGG 0.362152 4.3:-19843034 None:intergenic
CACGATGGTCCTGCATATCT+TGG 0.377345 4.3:+19842701 MS.gene28758:CDS
ATTGGATCAGACGGTGGTCT+TGG 0.412575 4.3:+19842905 MS.gene28758:CDS
ACTGTAAACTGCATCCATCT+TGG 0.469118 4.3:+19842804 MS.gene28758:CDS
TCGTGATAATCATCTGGAAC+AGG 0.478738 4.3:-19842683 None:intergenic
TTGCCTTTAATTTAATGGAA+AGG 0.482467 4.3:+19842645 None:intergenic
GTCCTGCATATCTTGGTTCG+TGG 0.485547 4.3:+19842708 MS.gene28758:CDS
GGACCATCGTGATAATCATC+TGG 0.487786 4.3:-19842689 None:intergenic
TTGGTGAAGTATAATGATAA+AGG 0.492510 4.3:+19842929 MS.gene28758:CDS
TAGCCGTACACTTAAAATAT+GGG 0.498718 4.3:+19842772 MS.gene28758:CDS
CTCTGTATACACGATCGCTT+GGG 0.514295 4.3:-19842996 None:intergenic
ACTCTGTATACACGATCGCT+TGG 0.527545 4.3:-19842997 None:intergenic
ACAAGAGTCTTAGTCCAAGA+TGG 0.541795 4.3:-19842818 None:intergenic
ATCAGACGGTGGTCTTGGAT+TGG 0.544899 4.3:+19842910 MS.gene28758:CDS
ACCCACGAACCAAGATATGC+AGG 0.553874 4.3:-19842710 None:intergenic
TCCTGCATATCTTGGTTCGT+GGG 0.554694 4.3:+19842709 MS.gene28758:CDS
GGTGATATTATTGGATCAGA+CGG 0.605256 4.3:+19842896 MS.gene28758:CDS
TAGAGGGGAAAATATGCGAA+TGG 0.624574 4.3:-19842858 None:intergenic
ACGATCGCTTGGGACCTAAG+TGG 0.637531 4.3:-19842986 None:intergenic
CCTACCGTGACTATCCACTT+AGG 0.665619 4.3:+19842972 MS.gene28758:CDS
GGGACCTAAGTGGATAGTCA+CGG 0.675794 4.3:-19842976 None:intergenic
GTTCCAGATGATTATCACGA+TGG 0.682457 4.3:+19842686 MS.gene28758:CDS
GATATTATTGGATCAGACGG+TGG 0.700053 4.3:+19842899 MS.gene28758:CDS
AAACTTGCTCTCTATCAACA+GGG 0.707501 4.3:-19843033 None:intergenic
CCTAAGTGGATAGTCACGGT+AGG 0.708448 4.3:-19842972 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACAAAAAGTGGTGATATTAT+TGG + chr4.3:19842887-19842906 MS.gene28758:CDS 25.0%
! TTGGTGAAGTATAATGATAA+AGG + chr4.3:19842929-19842948 MS.gene28758:CDS 25.0%
ATAGCCGTACACTTAAAATA+TGG + chr4.3:19842771-19842790 MS.gene28758:CDS 30.0%
TAGCCGTACACTTAAAATAT+GGG + chr4.3:19842772-19842791 MS.gene28758:CDS 30.0%
! ACTTTTTGTAGAACATAGAG+GGG - chr4.3:19842876-19842895 None:intergenic 30.0%
!! CACTTTTTGTAGAACATAGA+GGG - chr4.3:19842877-19842896 None:intergenic 30.0%
!! TCACCCATATTTTAAGTGTA+CGG - chr4.3:19842778-19842797 None:intergenic 30.0%
!! TGGAGAATTTTTCAGTCTAT+GGG + chr4.3:19842736-19842755 MS.gene28758:CDS 30.0%
!! TTGGAGAATTTTTCAGTCTA+TGG + chr4.3:19842735-19842754 MS.gene28758:CDS 30.0%
CCTCTATGTTCTACAAAAAG+TGG + chr4.3:19842875-19842894 MS.gene28758:CDS 35.0%
GGTGATATTATTGGATCAGA+CGG + chr4.3:19842896-19842915 MS.gene28758:CDS 35.0%
! AAACTTGCTCTCTATCAACA+GGG - chr4.3:19843036-19843055 None:intergenic 35.0%
! TAAACTTGCTCTCTATCAAC+AGG - chr4.3:19843037-19843056 None:intergenic 35.0%
!! ATTTTTCAGTCTATGGGCTA+TGG + chr4.3:19842742-19842761 MS.gene28758:CDS 35.0%
!!! CCACTTTTTGTAGAACATAG+AGG - chr4.3:19842878-19842897 None:intergenic 35.0%
ACAAGAGTCTTAGTCCAAGA+TGG - chr4.3:19842821-19842840 None:intergenic 40.0%
ACTGTAAACTGCATCCATCT+TGG + chr4.3:19842804-19842823 MS.gene28758:CDS 40.0%
GATATTATTGGATCAGACGG+TGG + chr4.3:19842899-19842918 MS.gene28758:CDS 40.0%
GTTCCAGATGATTATCACGA+TGG + chr4.3:19842686-19842705 MS.gene28758:CDS 40.0%
TAGAGGGGAAAATATGCGAA+TGG - chr4.3:19842861-19842880 None:intergenic 40.0%
! TATCTTGGTTCGTGGGTATT+TGG + chr4.3:19842716-19842735 MS.gene28758:CDS 40.0%
! TCGTGATAATCATCTGGAAC+AGG - chr4.3:19842686-19842705 None:intergenic 40.0%
ACTCTGTATACACGATCGCT+TGG - chr4.3:19843000-19843019 None:intergenic 45.0%
CTCTGTATACACGATCGCTT+GGG - chr4.3:19842999-19843018 None:intergenic 45.0%
GGACCATCGTGATAATCATC+TGG - chr4.3:19842692-19842711 None:intergenic 45.0%
! TCCTGCATATCTTGGTTCGT+GGG + chr4.3:19842709-19842728 MS.gene28758:CDS 45.0%
ACCCACGAACCAAGATATGC+AGG - chr4.3:19842713-19842732 None:intergenic 50.0%
ATTGGATCAGACGGTGGTCT+TGG + chr4.3:19842905-19842924 MS.gene28758:CDS 50.0%
CACGATGGTCCTGCATATCT+TGG + chr4.3:19842701-19842720 MS.gene28758:CDS 50.0%
CCTAAGTGGATAGTCACGGT+AGG - chr4.3:19842975-19842994 None:intergenic 50.0%
CCTACCGTGACTATCCACTT+AGG + chr4.3:19842972-19842991 MS.gene28758:CDS 50.0%
GGGACCTAAGTGGATAGTCA+CGG - chr4.3:19842979-19842998 None:intergenic 50.0%
! ATCAGACGGTGGTCTTGGAT+TGG + chr4.3:19842910-19842929 MS.gene28758:CDS 50.0%
! GTCCTGCATATCTTGGTTCG+TGG + chr4.3:19842708-19842727 MS.gene28758:CDS 50.0%
ACGATCGCTTGGGACCTAAG+TGG - chr4.3:19842989-19843008 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 19842659 19843057 19842659 ID=MS.gene28758
chr4.3 mRNA 19842659 19843057 19842659 ID=MS.gene28758.t1;Parent=MS.gene28758
chr4.3 exon 19842659 19843057 19842659 ID=MS.gene28758.t1.exon1;Parent=MS.gene28758.t1
chr4.3 CDS 19842659 19843057 19842659 ID=cds.MS.gene28758.t1;Parent=MS.gene28758.t1
Gene Sequence

>MS.gene28758

ATGGAAAGGAAACTGTTAGAGATACCTGTTCCAGATGATTATCACGATGGTCCTGCATATCTTGGTTCGTGGGTATTTGGAGAATTTTTCAGTCTATGGGCTATGGATCATTATAGCCGTACACTTAAAATATGGGTGATGAATGACTGTAAACTGCATCCATCTTGGACTAAGACTCTTGTTGTTCGTATTGATGATGCCATTCGCATATTTTCCCCTCTATGTTCTACAAAAAGTGGTGATATTATTGGATCAGACGGTGGTCTTGGATTGGTGAAGTATAATGATAAAGGACAACTCTTAGAGTATTGCGCCTACCGTGACTATCCACTTAGGTCCCAAGCGATCGTGTATACAGAGTCTCTGCTTTCACTCCCTGTTGATAGAGAGCAAGTTTAA

Protein sequence

>MS.gene28758.t1

MERKLLEIPVPDDYHDGPAYLGSWVFGEFFSLWAMDHYSRTLKIWVMNDCKLHPSWTKTLVVRIDDAIRIFSPLCSTKSGDIIGSDGGLGLVKYNDKGQLLEYCAYRDYPLRSQAIVYTESLLSLPVDREQV