AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070462

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070462.t1 MTR_3g034130 91.639 299 25 0 1 299 64 362 0.0 572
MS.gene070462.t1 MTR_3g034180 86.817 311 40 1 1 311 178 487 0.0 551
MS.gene070462.t1 MTR_3g034160 86.452 310 41 1 1 310 64 372 0.0 530
MS.gene070462.t1 MTR_3g103020 62.620 313 102 6 3 311 75 376 1.33e-128 372
MS.gene070462.t1 MTR_4g117720 60.312 320 103 9 1 310 70 375 8.23e-121 352
MS.gene070462.t1 MTR_6g060650 59.190 321 110 6 1 311 62 371 1.35e-117 343
MS.gene070462.t1 MTR_4g109270 57.729 317 110 8 1 311 64 362 9.07e-110 323
MS.gene070462.t1 MTR_4g109280 59.554 314 108 8 1 311 60 357 5.79e-105 311
MS.gene070462.t1 MTR_4g122850 53.968 315 130 8 2 311 58 362 4.09e-104 309
MS.gene070462.t1 MTR_4g117850 54.662 311 115 10 7 310 85 376 1.07e-97 293
MS.gene070462.t1 MTR_4g118380 53.746 307 110 6 1 286 62 357 7.94e-96 287
MS.gene070462.t1 MTR_4g117770 54.952 313 113 7 9 311 71 365 8.94e-96 288
MS.gene070462.t1 MTR_4g117810 54.952 313 113 7 9 311 71 365 8.94e-96 288
MS.gene070462.t1 MTR_4g094365 51.735 317 130 8 1 311 63 362 3.93e-94 283
MS.gene070462.t1 MTR_4g023080 52.396 313 123 8 1 311 64 352 2.29e-93 281
MS.gene070462.t1 MTR_5g097210 49.838 309 139 6 3 310 67 360 1.09e-90 275
MS.gene070462.t1 MTR_4g117780 50.779 321 127 8 1 311 66 365 6.56e-90 273
MS.gene070462.t1 MTR_4g023090 48.466 326 133 8 1 308 71 379 2.72e-89 271
MS.gene070462.t1 MTR_4g116330 51.623 308 126 8 10 310 86 377 6.36e-89 271
MS.gene070462.t1 MTR_4g021785 50.489 307 127 7 7 311 70 353 2.23e-87 266
MS.gene070462.t1 MTR_5g070080 49.085 328 130 7 4 308 67 380 1.79e-86 265
MS.gene070462.t1 MTR_5g070130 49.533 321 136 10 3 310 67 374 5.11e-85 261
MS.gene070462.t1 MTR_4g117750 49.527 317 129 8 3 309 60 355 3.19e-84 262
MS.gene070462.t1 MTR_4g117750 58.716 109 43 1 205 311 373 481 1.04e-32 126
MS.gene070462.t1 MTR_4g023070 48.012 327 138 10 1 311 58 368 3.43e-84 258
MS.gene070462.t1 MTR_4g023380 47.447 333 115 9 7 311 77 377 5.35e-82 253
MS.gene070462.t1 MTR_4g117870 48.710 310 111 7 1 309 1 263 3.05e-81 247
MS.gene070462.t1 MTR_1g055135 46.645 313 147 8 7 311 73 373 4.97e-81 250
MS.gene070462.t1 MTR_1g055195 49.498 299 120 7 20 310 200 475 1.08e-80 253
MS.gene070462.t1 MTR_4g023120 47.706 327 139 11 1 311 58 368 2.98e-80 248
MS.gene070462.t1 MTR_4g023190 49.371 318 129 11 1 311 65 357 1.90e-79 246
MS.gene070462.t1 MTR_4g023130 50.523 287 118 7 27 311 47 311 2.91e-79 244
MS.gene070462.t1 MTR_8g433040 49.206 315 127 9 4 311 74 362 6.40e-79 244
MS.gene070462.t1 MTR_8g032380 49.206 315 127 9 4 311 74 362 6.40e-79 244
MS.gene070462.t1 MTR_4g023200 47.799 318 135 11 1 311 65 358 9.85e-77 239
MS.gene070462.t1 MTR_5g070088 44.985 329 147 7 3 308 63 380 8.06e-76 237
MS.gene070462.t1 MTR_4g023150 46.688 317 140 10 1 311 65 358 1.19e-74 233
MS.gene070462.t1 MTR_4g118400 46.844 301 112 8 16 307 33 294 1.34e-73 228
MS.gene070462.t1 MTR_8g032390 47.152 316 119 11 2 308 94 370 4.00e-73 230
MS.gene070462.t1 MTR_4g116320 57.143 224 81 6 91 310 43 255 4.07e-73 226
MS.gene070462.t1 MTR_4g023440 44.695 311 118 7 1 311 74 330 5.76e-72 226
MS.gene070462.t1 MTR_8g013010 57.407 216 75 6 1 214 109 309 2.31e-69 218
MS.gene070462.t1 MTR_4g118370 44.912 285 125 7 28 310 3 257 6.42e-66 207
MS.gene070462.t1 MTR_8g040935 53.081 211 87 4 107 311 2 206 4.76e-64 201
MS.gene070462.t1 MTR_7g079400 41.042 307 158 8 14 311 43 335 3.60e-62 201
MS.gene070462.t1 MTR_8g433030 53.304 227 87 8 87 308 125 337 5.67e-62 200
MS.gene070462.t1 MTR_4g116330 43.506 308 112 9 10 310 86 338 1.60e-60 196
MS.gene070462.t1 MTR_7g079370 42.188 320 149 11 7 307 48 350 4.69e-60 196
MS.gene070462.t1 MTR_4g121580 43.791 306 122 13 7 303 75 339 2.45e-55 184
MS.gene070462.t1 MTR_1g055165 39.744 312 124 8 3 311 66 316 1.66e-54 180
MS.gene070462.t1 MTR_8g102520 39.365 315 139 11 7 311 48 320 3.73e-52 174
MS.gene070462.t1 MTR_4g117530 43.191 257 86 8 63 311 47 251 7.65e-52 171
MS.gene070462.t1 MTR_1g055175 48.485 198 92 5 118 311 8 199 3.20e-51 168
MS.gene070462.t1 MTR_6g463430 48.077 208 92 5 109 311 4 200 2.13e-50 166
MS.gene070462.t1 MTR_1g055250 42.424 231 118 8 74 300 34 253 1.63e-49 167
MS.gene070462.t1 MTR_4g117430 52.761 163 54 5 57 217 14 155 1.21e-46 154
MS.gene070462.t1 MTR_4g114700 39.405 269 102 8 42 304 5 218 1.71e-41 144
MS.gene070462.t1 MTR_4g116340 48.295 176 75 5 10 180 83 247 4.57e-40 140
MS.gene070462.t1 MTR_4g118360 59.200 125 45 3 190 310 1 123 9.74e-40 136
MS.gene070462.t1 MTR_2g048580 32.131 305 161 11 10 308 73 337 7.89e-38 137
MS.gene070462.t1 MTR_7g079420 35.587 281 124 11 45 311 905 1142 8.63e-37 140
MS.gene070462.t1 MTR_8g098830 42.529 174 86 5 114 282 30 194 3.13e-36 130
MS.gene070462.t1 MTR_5g023000 63.441 93 34 0 219 311 5 97 9.34e-32 114
MS.gene070462.t1 MTR_4g122860 39.698 199 74 7 123 311 156 318 3.24e-30 117
MS.gene070462.t1 MTR_6g034720 45.860 157 64 6 40 193 31 169 8.28e-29 110
MS.gene070462.t1 MTR_7g117720 50.000 144 56 7 7 145 52 184 1.97e-28 110
MS.gene070462.t1 MTR_7g117720 40.000 150 76 6 164 308 132 272 5.19e-16 77.0
MS.gene070462.t1 MTR_7g079450 41.611 149 62 5 174 311 1 135 7.02e-25 97.8
MS.gene070462.t1 MTR_1g068875 27.350 234 144 8 23 243 71 291 7.93e-22 94.7
MS.gene070462.t1 MTR_4g122840 42.537 134 39 4 181 311 164 262 5.10e-20 87.8
MS.gene070462.t1 MTR_5g069120 44.186 129 63 2 6 127 51 177 1.23e-18 82.0
MS.gene070462.t1 MTR_0667s0010 24.399 291 179 10 6 268 57 334 1.56e-18 85.9
MS.gene070462.t1 MTR_4g133170 30.275 218 123 12 36 238 82 285 5.03e-18 84.0
MS.gene070462.t1 MTR_2g026010 28.444 225 136 9 35 242 81 297 1.55e-17 82.8
MS.gene070462.t1 MTR_4g023430 56.250 80 32 1 235 311 141 220 2.33e-17 79.7
MS.gene070462.t1 MTR_2g024110 24.719 267 167 11 7 259 60 306 2.58e-17 82.0
MS.gene070462.t1 MTR_1g068835 27.119 236 143 10 23 243 71 292 3.84e-17 81.6
MS.gene070462.t1 MTR_2g028130 26.800 250 145 10 37 261 100 336 6.51e-17 80.9
MS.gene070462.t1 MTR_8g013005 66.667 57 19 0 255 311 1 57 6.58e-17 73.9
MS.gene070462.t1 MTR_2g028160 27.193 228 138 9 57 268 130 345 8.22e-17 80.9
MS.gene070462.t1 MTR_2g426020 23.860 285 175 10 19 276 72 341 1.88e-16 79.7
MS.gene070462.t1 MTR_2g426070 26.336 262 151 12 39 277 101 343 1.99e-16 79.3
MS.gene070462.t1 MTR_1g055200 50.000 86 38 2 42 127 5 85 2.35e-16 73.9
MS.gene070462.t1 MTR_4g070550 25.974 231 134 8 34 238 92 311 3.54e-16 79.0
MS.gene070462.t1 MTR_2g031640 25.214 234 150 7 24 242 72 295 5.25e-16 78.2
MS.gene070462.t1 MTR_2g025960 26.201 229 130 9 39 242 1 215 5.84e-16 77.4
MS.gene070462.t1 MTR_2g021155 28.804 184 115 5 59 238 711 882 9.21e-16 78.2
MS.gene070462.t1 MTR_2g021155 30.732 205 114 10 44 238 131 317 1.36e-12 68.9
MS.gene070462.t1 MTR_4g055060 29.583 240 140 10 16 243 100 322 1.66e-15 76.6
MS.gene070462.t1 MTR_2g078870 30.220 182 102 7 59 240 78 234 2.27e-15 75.5
MS.gene070462.t1 MTR_1g068860 25.106 235 151 7 23 243 71 294 4.24e-15 75.5
MS.gene070462.t1 MTR_2g028140 25.339 221 128 8 44 238 108 317 1.59e-14 73.9
MS.gene070462.t1 MTR_1g054725 27.857 280 164 15 43 305 117 375 7.59e-14 71.6
MS.gene070462.t1 MTR_3g010970 29.189 185 97 7 58 242 170 320 9.76e-14 71.6
MS.gene070462.t1 MTR_8g464770 26.367 311 188 14 17 307 88 377 1.01e-13 71.2
MS.gene070462.t1 MTR_2g031680 25.833 240 151 8 17 242 66 292 1.22e-13 71.2
MS.gene070462.t1 MTR_6g053260 28.302 212 125 8 34 238 429 620 1.57e-13 71.6
MS.gene070462.t1 MTR_3g007330 27.488 211 122 7 44 243 138 328 1.91e-13 70.5
MS.gene070462.t1 MTR_2g078900 29.348 184 103 8 59 240 153 311 2.60e-13 70.1
MS.gene070462.t1 MTR_3g083640 27.477 222 128 9 23 238 136 330 2.94e-13 70.1
MS.gene070462.t1 MTR_2g425980 28.431 204 117 8 56 242 121 312 3.74e-13 69.7
MS.gene070462.t1 MTR_7g070830 25.424 236 141 11 57 264 147 375 3.85e-13 69.7
MS.gene070462.t1 MTR_2g021150 27.132 258 164 9 15 264 87 328 6.42e-13 68.9
MS.gene070462.t1 MTR_1g094210 29.457 258 137 13 41 284 152 378 6.50e-13 69.3
MS.gene070462.t1 MTR_2g461450 26.941 219 134 8 32 243 97 296 7.23e-13 68.6
MS.gene070462.t1 MTR_3g009310 27.461 193 111 6 57 243 158 327 8.27e-13 68.9
MS.gene070462.t1 MTR_3g007430 27.982 218 130 8 32 238 123 324 9.82e-13 68.6
MS.gene070462.t1 MTR_3g010920 25.385 260 146 9 58 305 158 381 9.85e-13 68.6
MS.gene070462.t1 MTR_2g021190 26.522 230 148 8 15 238 90 304 1.14e-12 68.2
MS.gene070462.t1 MTR_2g024100 25.806 248 141 10 20 238 73 306 1.28e-12 68.2
MS.gene070462.t1 MTR_4g030830 24.092 303 167 13 44 305 113 393 1.59e-12 67.8
MS.gene070462.t1 MTR_2g025420 24.272 309 176 13 6 278 67 353 1.64e-12 67.8
MS.gene070462.t1 MTR_0044s0300 27.885 208 111 11 44 240 138 317 1.95e-12 67.8
MS.gene070462.t1 MTR_6g065980 28.000 200 115 9 44 240 111 284 3.14e-12 67.0
MS.gene070462.t1 MTR_2g031720 23.394 218 143 6 39 242 92 299 4.32e-12 66.6
MS.gene070462.t1 MTR_3g007410 26.794 209 129 7 44 243 146 339 4.81e-12 66.6
MS.gene070462.t1 MTR_3g006570 24.912 285 165 12 44 305 118 376 6.29e-12 66.2
MS.gene070462.t1 MTR_3g007360 26.540 211 127 7 44 243 146 339 6.51e-12 66.2
MS.gene070462.t1 MTR_2g078850 29.126 206 113 10 44 240 130 311 6.70e-12 65.9
MS.gene070462.t1 MTR_1g094210 29.365 252 133 13 41 278 152 372 1.04e-11 65.5
MS.gene070462.t1 MTR_2g021252 25.726 241 145 8 3 238 105 316 1.26e-11 65.1
MS.gene070462.t1 MTR_3g024130 28.169 213 124 10 41 243 58 251 1.41e-11 64.7
MS.gene070462.t1 MTR_8g099460 22.634 243 151 8 23 242 73 301 1.62e-11 65.1
MS.gene070462.t1 MTR_3g013740 27.586 203 125 6 44 238 150 338 1.81e-11 64.7
MS.gene070462.t1 MTR_4g031770 26.549 226 123 10 31 238 85 285 2.11e-11 64.3
MS.gene070462.t1 MTR_3g010870 26.829 205 119 8 41 238 131 311 2.46e-11 64.3
MS.gene070462.t1 MTR_3g030250 27.807 187 103 7 58 242 154 310 2.78e-11 63.9
MS.gene070462.t1 MTR_4g023100 62.963 54 18 1 257 310 65 116 3.52e-11 60.1
MS.gene070462.t1 MTR_6g027890 25.833 240 145 7 19 238 30 256 4.05e-11 63.2
MS.gene070462.t1 MTR_4g109290 59.615 52 21 0 260 311 135 186 5.21e-11 61.2
MS.gene070462.t1 MTR_2g040050 23.305 236 138 10 35 256 34 240 6.10e-11 62.0
MS.gene070462.t1 MTR_1g097560 24.555 281 179 11 7 281 99 352 6.28e-11 63.2
MS.gene070462.t1 MTR_6g007135 28.571 217 126 10 38 242 121 320 7.17e-11 62.8
MS.gene070462.t1 MTR_4g117550 27.239 268 144 12 57 308 139 371 8.45e-11 62.8
MS.gene070462.t1 MTR_4g028510 26.804 291 159 12 53 310 133 402 9.41e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070462.t1 AT4G12560 30.374 214 132 9 40 239 90 300 8.14e-16 77.8
MS.gene070462.t1 AT4G12560 30.374 214 132 9 40 239 90 300 8.14e-16 77.8
MS.gene070462.t1 AT4G12560 30.374 214 132 9 40 239 90 300 8.14e-16 77.8

Find 68 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAGAATTTCTCAGTCTA+TGG 0.212417 3.3:-24954111 MS.gene070462:CDS
ACAAAATGTGGTCATATTAT+TGG 0.222453 3.3:-24953962 MS.gene070462:CDS
GATTGTGATTTGTGGATATT+TGG 0.226311 3.3:-24954130 MS.gene070462:CDS
TACTCACTTCCCTTATTATA+TGG 0.240722 3.3:-24954314 MS.gene070462:CDS
GAGTCTCTGCTTTCACTTCC+TGG 0.246781 3.3:-24953833 MS.gene070462:CDS
AAATATTTATGTTGTCTATA+TGG 0.249954 3.3:-24954496 MS.gene070462:CDS
AGGATGAAACAATTGAAATA+TGG 0.254108 3.3:-24954081 MS.gene070462:CDS
TCATTCGCGTCCATATAATA+AGG 0.269202 3.3:+24954304 None:intergenic
GATGAAATATTATCTTCTAT+TGG 0.305333 3.3:+24954406 None:intergenic
TTATGTTGTCTATATGGTTT+TGG 0.316449 3.3:-24954490 MS.gene070462:CDS
ATGAAATATTATCTTCTATT+GGG 0.319010 3.3:+24954407 None:intergenic
AAGCGATGCTTCAAAATCTA+TGG 0.346335 3.3:+24954705 None:intergenic
ATTAAAGAGCGTCCCTACTT+TGG 0.356100 3.3:+24954276 None:intergenic
TTCAAGATTAGAATAAGATT+CGG 0.367334 3.3:+24954636 None:intergenic
TATTGTTGGCTTTGATTTAG+TGG 0.370118 3.3:-24954206 MS.gene070462:CDS
TTAAAGAGCGTCCCTACTTT+GGG 0.372779 3.3:+24954277 None:intergenic
CAACTCCAGCTCTTGAAATT+CGG 0.394717 3.3:-24954729 MS.gene070462:CDS
TCAAGATTAGAATAAGATTC+GGG 0.401464 3.3:+24954637 None:intergenic
CCATTCAACAGATGATTACT+TGG 0.402778 3.3:-24954461 MS.gene070462:CDS
ACTCATGTTCTATCTATTGA+TGG 0.411572 3.3:-24954019 MS.gene070462:CDS
AACCTATAGATTGTGATTTG+TGG 0.425796 3.3:-24954138 MS.gene070462:CDS
ATTCTTTACATGTCTACCTA+TGG 0.432569 3.3:-24954576 MS.gene070462:CDS
GGATGAAACAATTGAAATAT+GGG 0.439115 3.3:-24954080 MS.gene070462:CDS
ATTGGAACAAATGGTGGTAC+TGG 0.446274 3.3:-24953944 MS.gene070462:CDS
AATCACAATCTATAGGTTCA+TGG 0.456461 3.3:+24954143 None:intergenic
GGTTCATGGTCAAAACCATC+TGG 0.456773 3.3:+24954157 None:intergenic
TCTTTAATGAGACTATTCAT+TGG 0.456901 3.3:-24954261 MS.gene070462:CDS
ATCCACAAATCACAATCTAT+AGG 0.460799 3.3:+24954136 None:intergenic
TCTAAATTGGAAGGAGGAAA+AGG 0.467688 3.3:+24954523 None:intergenic
TTATCAATGGATGTTATTGT+TGG 0.479970 3.3:-24954220 MS.gene070462:CDS
TGGGTCTATACTTGTATCAT+AGG 0.480906 3.3:+24954426 None:intergenic
CATTCGCGTCCATATAATAA+GGG 0.483397 3.3:+24954305 None:intergenic
TAACATCCATTGATAAATCA+TGG 0.489522 3.3:+24954227 None:intergenic
TGGAGAATTTCTCAGTCTAT+GGG 0.490268 3.3:-24954110 MS.gene070462:CDS
CTCTGTATACATAGCCACTA+GGG 0.491428 3.3:+24953853 None:intergenic
TCTCGTTGAGAGCTAATACA+TGG 0.498785 3.3:-24954369 MS.gene070462:CDS
GGTCATATTATTGGAACAAA+TGG 0.505789 3.3:-24953953 MS.gene070462:CDS
ATTTCTCAGTCTATGGGCTA+TGG 0.507502 3.3:-24954104 MS.gene070462:CDS
CTCATGTTCTATCTATTGAT+GGG 0.515925 3.3:-24954018 MS.gene070462:CDS
ATGGACCGAATTTCAAGAGC+TGG 0.519399 3.3:+24954724 None:intergenic
AGTACAAAGTGCATTCGTCT+TGG 0.521606 3.3:-24954048 MS.gene070462:CDS
CCAAGTAATCATCTGTTGAA+TGG 0.522116 3.3:+24954461 None:intergenic
GGAACGAAGTTGAGTGTCCC+GGG 0.527311 3.3:-24954348 MS.gene070462:CDS
GAAATTAAAAGTTCATGTAG+AGG 0.538733 3.3:-24954616 MS.gene070462:CDS
TGGAACGAAGTTGAGTGTCC+CGG 0.545977 3.3:-24954349 MS.gene070462:CDS
GCGAATGATGATCCCAAAGT+AGG 0.546958 3.3:-24954289 MS.gene070462:CDS
TAAATTTACTCACTGTCACC+AGG 0.559531 3.3:+24953815 None:intergenic
TACCTATGGAATCCATCCAC+TGG 0.560035 3.3:-24954562 MS.gene070462:CDS
TTGGCTTTGATTTAGTGGAA+AGG 0.565737 3.3:-24954201 MS.gene070462:CDS
TTCAACAGATGATTACTTGG+TGG 0.569107 3.3:-24954458 MS.gene070462:CDS
GTGAGTATACTTCACCAACC+CGG 0.571090 3.3:+24954330 None:intergenic
CGAATGATGATCCCAAAGTA+GGG 0.573102 3.3:-24954288 MS.gene070462:CDS
CCAATATGCTCTACAAAATG+TGG 0.574609 3.3:-24953974 MS.gene070462:CDS
TCTCAGTCTATGGGCTATGG+AGG 0.578722 3.3:-24954101 MS.gene070462:CDS
TGTTTGTGACGTCCAGTGGA+TGG 0.578837 3.3:+24954550 None:intergenic
TGAGTATACTTCACCAACCC+GGG 0.588228 3.3:+24954331 None:intergenic
CATATTATTGGAACAAATGG+TGG 0.593852 3.3:-24953950 MS.gene070462:CDS
TTTGCATCTAAATTGGAAGG+AGG 0.595652 3.3:+24954517 None:intergenic
TATTTGTTTGTGACGTCCAG+TGG 0.604020 3.3:+24954546 None:intergenic
CGAAGTTGAGTGTCCCGGGT+TGG 0.604087 3.3:-24954344 MS.gene070462:CDS
AACAAATGGTGGTACTGGAT+TGG 0.613049 3.3:-24953939 MS.gene070462:CDS
ACTCTGTATACATAGCCACT+AGG 0.618200 3.3:+24953852 None:intergenic
TGAACCATGTGCACCCCTAG+TGG 0.636728 3.3:-24953867 MS.gene070462:CDS
ATAGCCACTAGGGGTGCACA+TGG 0.643218 3.3:+24953863 None:intergenic
GTCCAGTGGATGGATTCCAT+AGG 0.672791 3.3:+24954560 None:intergenic
TCTGTATACATAGCCACTAG+GGG 0.698983 3.3:+24953854 None:intergenic
TTGGTGAAGTACGATGGCAA+TGG 0.721153 3.3:-24953920 MS.gene070462:CDS
ACTGGATTGGTGAAGTACGA+TGG 0.727911 3.3:-24953926 MS.gene070462:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATTTATGTTGTCTATA+TGG - chr3.3:24954057-24954076 MS.gene070462:CDS 15.0%
!! ATGAAATATTATCTTCTATT+GGG + chr3.3:24954149-24954168 None:intergenic 15.0%
!!! TAAATATTTTGCATCTAAAT+TGG + chr3.3:24954046-24954065 None:intergenic 15.0%
!! GATGAAATATTATCTTCTAT+TGG + chr3.3:24954150-24954169 None:intergenic 20.0%
!! TTCAAGATTAGAATAAGATT+CGG + chr3.3:24953920-24953939 None:intergenic 20.0%
! ACAAAATGTGGTCATATTAT+TGG - chr3.3:24954591-24954610 MS.gene070462:CDS 25.0%
! AGGATGAAACAATTGAAATA+TGG - chr3.3:24954472-24954491 MS.gene070462:CDS 25.0%
! GAAATTAAAAGTTCATGTAG+AGG - chr3.3:24953937-24953956 MS.gene070462:CDS 25.0%
! GGATGAAACAATTGAAATAT+GGG - chr3.3:24954473-24954492 MS.gene070462:CDS 25.0%
! TAACATCCATTGATAAATCA+TGG + chr3.3:24954329-24954348 None:intergenic 25.0%
! TCAAGATTAGAATAAGATTC+GGG + chr3.3:24953919-24953938 None:intergenic 25.0%
! TCTTTAATGAGACTATTCAT+TGG - chr3.3:24954292-24954311 MS.gene070462:CDS 25.0%
! TTATCAATGGATGTTATTGT+TGG - chr3.3:24954333-24954352 MS.gene070462:CDS 25.0%
!! TATTTTGCATCTAAATTGGA+AGG + chr3.3:24954042-24954061 None:intergenic 25.0%
!! TTATGTTGTCTATATGGTTT+TGG - chr3.3:24954063-24954082 MS.gene070462:CDS 25.0%
AACCTATAGATTGTGATTTG+TGG - chr3.3:24954415-24954434 MS.gene070462:CDS 30.0%
AATCACAATCTATAGGTTCA+TGG + chr3.3:24954413-24954432 None:intergenic 30.0%
ACTCATGTTCTATCTATTGA+TGG - chr3.3:24954534-24954553 MS.gene070462:CDS 30.0%
ATCCACAAATCACAATCTAT+AGG + chr3.3:24954420-24954439 None:intergenic 30.0%
ATTCTTTACATGTCTACCTA+TGG - chr3.3:24953977-24953996 MS.gene070462:CDS 30.0%
CATATTATTGGAACAAATGG+TGG - chr3.3:24954603-24954622 MS.gene070462:CDS 30.0%
CTCATGTTCTATCTATTGAT+GGG - chr3.3:24954535-24954554 MS.gene070462:CDS 30.0%
GGTCATATTATTGGAACAAA+TGG - chr3.3:24954600-24954619 MS.gene070462:CDS 30.0%
TACTCACTTCCCTTATTATA+TGG - chr3.3:24954239-24954258 MS.gene070462:CDS 30.0%
! ACATTTTGTAGAGCATATTG+GGG + chr3.3:24954580-24954599 None:intergenic 30.0%
! CACATTTTGTAGAGCATATT+GGG + chr3.3:24954581-24954600 None:intergenic 30.0%
! GATTGTGATTTGTGGATATT+TGG - chr3.3:24954423-24954442 MS.gene070462:CDS 30.0%
! TTTTCGCCATGATTTATCAA+TGG - chr3.3:24954320-24954339 MS.gene070462:CDS 30.0%
!! TATTGTTGGCTTTGATTTAG+TGG - chr3.3:24954347-24954366 MS.gene070462:CDS 30.0%
AAGCGATGCTTCAAAATCTA+TGG + chr3.3:24953851-24953870 None:intergenic 35.0%
CATTCGCGTCCATATAATAA+GGG + chr3.3:24954251-24954270 None:intergenic 35.0%
CCAAGTAATCATCTGTTGAA+TGG + chr3.3:24954095-24954114 None:intergenic 35.0%
CCAATATGCTCTACAAAATG+TGG - chr3.3:24954579-24954598 MS.gene070462:CDS 35.0%
CCATTCAACAGATGATTACT+TGG - chr3.3:24954092-24954111 MS.gene070462:CDS 35.0%
TCATTCGCGTCCATATAATA+AGG + chr3.3:24954252-24954271 None:intergenic 35.0%
TCTAAATTGGAAGGAGGAAA+AGG + chr3.3:24954033-24954052 None:intergenic 35.0%
TGGAGAATTTCTCAGTCTAT+GGG - chr3.3:24954443-24954462 MS.gene070462:CDS 35.0%
TGGGTCTATACTTGTATCAT+AGG + chr3.3:24954130-24954149 None:intergenic 35.0%
TTCAACAGATGATTACTTGG+TGG - chr3.3:24954095-24954114 MS.gene070462:CDS 35.0%
TTGGAGAATTTCTCAGTCTA+TGG - chr3.3:24954442-24954461 MS.gene070462:CDS 35.0%
TTTGCATCTAAATTGGAAGG+AGG + chr3.3:24954039-24954058 None:intergenic 35.0%
! CATTTTGTAGAGCATATTGG+GGG + chr3.3:24954579-24954598 None:intergenic 35.0%
! CCACATTTTGTAGAGCATAT+TGG + chr3.3:24954582-24954601 None:intergenic 35.0%
! TTAGAGATGCATTTTCCAGA+TGG - chr3.3:24954381-24954400 MS.gene070462:CDS 35.0%
!! TTGGCTTTGATTTAGTGGAA+AGG - chr3.3:24954352-24954371 MS.gene070462:CDS 35.0%
ACTCTGTATACATAGCCACT+AGG + chr3.3:24954704-24954723 None:intergenic 40.0%
AGTACAAAGTGCATTCGTCT+TGG - chr3.3:24954505-24954524 MS.gene070462:CDS 40.0%
ATTGGAACAAATGGTGGTAC+TGG - chr3.3:24954609-24954628 MS.gene070462:CDS 40.0%
CAACTCCAGCTCTTGAAATT+CGG - chr3.3:24953824-24953843 MS.gene070462:CDS 40.0%
CGAATGATGATCCCAAAGTA+GGG - chr3.3:24954265-24954284 MS.gene070462:CDS 40.0%
CTCTGTATACATAGCCACTA+GGG + chr3.3:24954703-24954722 None:intergenic 40.0%
TATTTGTTTGTGACGTCCAG+TGG + chr3.3:24954010-24954029 None:intergenic 40.0%
TCTCGTTGAGAGCTAATACA+TGG - chr3.3:24954184-24954203 MS.gene070462:CDS 40.0%
TCTGTATACATAGCCACTAG+GGG + chr3.3:24954702-24954721 None:intergenic 40.0%
! AACAAATGGTGGTACTGGAT+TGG - chr3.3:24954614-24954633 MS.gene070462:CDS 40.0%
! ATTTCTCAGTCTATGGGCTA+TGG - chr3.3:24954449-24954468 MS.gene070462:CDS 40.0%
!! ATTAAAGAGCGTCCCTACTT+TGG + chr3.3:24954280-24954299 None:intergenic 40.0%
!! TTAAAGAGCGTCCCTACTTT+GGG + chr3.3:24954279-24954298 None:intergenic 40.0%
ATGGACCGAATTTCAAGAGC+TGG + chr3.3:24953832-24953851 None:intergenic 45.0%
GCGAATGATGATCCCAAAGT+AGG - chr3.3:24954264-24954283 MS.gene070462:CDS 45.0%
GGTTCATGGTCAAAACCATC+TGG + chr3.3:24954399-24954418 None:intergenic 45.0%
GTGAGTATACTTCACCAACC+CGG + chr3.3:24954226-24954245 None:intergenic 45.0%
TACCTATGGAATCCATCCAC+TGG - chr3.3:24953991-24954010 MS.gene070462:CDS 45.0%
TGAGTATACTTCACCAACCC+GGG + chr3.3:24954225-24954244 None:intergenic 45.0%
TTGGTGAAGTACGATGGCAA+TGG - chr3.3:24954633-24954652 MS.gene070462:CDS 45.0%
!! ACTGGATTGGTGAAGTACGA+TGG - chr3.3:24954627-24954646 MS.gene070462:CDS 45.0%
GAGTCTCTGCTTTCACTTCC+TGG - chr3.3:24954720-24954739 MS.gene070462:CDS 50.0%
TGGAACGAAGTTGAGTGTCC+CGG - chr3.3:24954204-24954223 MS.gene070462:CDS 50.0%
TGTTTGTGACGTCCAGTGGA+TGG + chr3.3:24954006-24954025 None:intergenic 50.0%
! GTCCAGTGGATGGATTCCAT+AGG + chr3.3:24953996-24954015 None:intergenic 50.0%
! TCTCAGTCTATGGGCTATGG+AGG - chr3.3:24954452-24954471 MS.gene070462:CDS 50.0%
GGAACGAAGTTGAGTGTCCC+GGG - chr3.3:24954205-24954224 MS.gene070462:CDS 55.0%
TGAACCATGTGCACCCCTAG+TGG - chr3.3:24954686-24954705 MS.gene070462:CDS 55.0%
! ATAGCCACTAGGGGTGCACA+TGG + chr3.3:24954693-24954712 None:intergenic 55.0%
CGAAGTTGAGTGTCCCGGGT+TGG - chr3.3:24954209-24954228 MS.gene070462:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 24953820 24954755 24953820 ID=MS.gene070462
chr3.3 mRNA 24953820 24954755 24953820 ID=MS.gene070462.t1;Parent=MS.gene070462
chr3.3 exon 24953820 24954755 24953820 ID=MS.gene070462.t1.exon1;Parent=MS.gene070462.t1
chr3.3 CDS 24953820 24954755 24953820 ID=cds.MS.gene070462.t1;Parent=MS.gene070462.t1
Gene Sequence

>MS.gene070462

ATGTCAACTCCAGCTCTTGAAATTCGGTCCATAGATTTTGAAGCATCGCTTCACGATGATTCTGCTTCTACTTCACTGAACTTGAATCTTACGCTTCCCGAATCTTATTCTAATCTTGAAATTAAAAGTTCATGTAGAGGATTTATAGTTTTGACTTATTCTTTACATGTCTACCTATGGAATCCATCCACTGGACGTCACAAACAAATACCTTTTCCTCCTTCCAATTTAGATGCAAAATATTTATGTTGTCTATATGGTTTTGGTTATGACCATTCAACAGATGATTACTTGGTGGTATCAGTGTCCTATGATACAAGTATAGACCCAATAGAAGATAATATTTCATCACATTTGAAGTTTTTCTCGTTGAGAGCTAATACATGGAACGAAGTTGAGTGTCCCGGGTTGGTGAAGTATACTCACTTCCCTTATTATATGGACGCGAATGATGATCCCAAAGTAGGGACGCTCTTTAATGAGACTATTCATTGGTTCTCTTTTCGCCATGATTTATCAATGGATGTTATTGTTGGCTTTGATTTAGTGGAAAGGAAACTTTTAGAGATGCATTTTCCAGATGGTTTTGACCATGAACCTATAGATTGTGATTTGTGGATATTTGGAGAATTTCTCAGTCTATGGGCTATGGAGGATGAAACAATTGAAATATGGGTTATGAAAGAGTACAAAGTGCATTCGTCTTGGACGAAGACTCATGTTCTATCTATTGATGGGATTTGCATTCAGTATGATCCCCCAATATGCTCTACAAAATGTGGTCATATTATTGGAACAAATGGTGGTACTGGATTGGTGAAGTACGATGGCAATGGTCAGTTGCTAGAGAATCGCTCATACTATAATGAACCATGTGCACCCCTAGTGGCTATGTATACAGAGTCTCTGCTTTCACTTCCTGGTGACAGTGAGTAA
Protein sequence

>MS.gene070462.t1

MSTPALEIRSIDFEASLHDDSASTSLNLNLTLPESYSNLEIKSSCRGFIVLTYSLHVYLWNPSTGRHKQIPFPPSNLDAKYLCCLYGFGYDHSTDDYLVVSVSYDTSIDPIEDNISSHLKFFSLRANTWNEVECPGLVKYTHFPYYMDANDDPKVGTLFNETIHWFSFRHDLSMDVIVGFDLVERKLLEMHFPDGFDHEPIDCDLWIFGEFLSLWAMEDETIEIWVMKEYKVHSSWTKTHVLSIDGICIQYDPPICSTKCGHIIGTNGGTGLVKYDGNGQLLENRSYYNEPCAPLVAMYTESLLSLPGDSE