AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064111

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064111.t1 MTR_4g023120 91.007 278 25 0 28 305 90 367 0.0 531
MS.gene064111.t1 MTR_4g023070 90.288 278 27 0 28 305 90 367 0.0 524
MS.gene064111.t1 MTR_4g023130 60.308 325 84 6 3 305 9 310 4.44e-122 352
MS.gene064111.t1 MTR_4g023090 63.345 281 93 3 28 303 104 379 1.30e-121 354
MS.gene064111.t1 MTR_4g021785 62.500 280 80 4 28 305 96 352 1.44e-116 340
MS.gene064111.t1 MTR_4g023080 62.724 279 80 4 28 305 96 351 9.45e-114 333
MS.gene064111.t1 MTR_4g023380 56.193 331 74 7 2 305 90 376 1.53e-111 328
MS.gene064111.t1 MTR_4g023150 56.940 281 98 5 28 305 97 357 1.48e-98 294
MS.gene064111.t1 MTR_4g023200 56.584 281 99 5 28 305 97 357 3.32e-98 293
MS.gene064111.t1 MTR_4g023190 57.295 281 96 6 28 305 97 356 1.34e-97 291
MS.gene064111.t1 MTR_4g117720 49.568 347 113 12 1 305 49 375 1.09e-92 280
MS.gene064111.t1 MTR_3g103020 55.363 289 98 9 28 305 107 375 1.90e-91 276
MS.gene064111.t1 MTR_4g023440 51.724 290 85 4 16 305 95 329 5.91e-90 271
MS.gene064111.t1 MTR_6g060650 52.669 281 106 8 33 305 109 370 2.88e-86 263
MS.gene064111.t1 MTR_4g116330 53.091 275 106 7 36 305 121 377 2.03e-85 261
MS.gene064111.t1 MTR_4g109280 53.521 284 108 7 28 305 91 356 3.30e-85 260
MS.gene064111.t1 MTR_4g094365 53.169 284 104 8 30 304 97 360 5.14e-85 260
MS.gene064111.t1 MTR_4g117850 51.042 288 110 9 29 305 109 376 1.09e-81 252
MS.gene064111.t1 MTR_8g032390 46.991 349 119 12 3 303 40 370 2.33e-80 248
MS.gene064111.t1 MTR_3g034160 52.218 293 107 10 28 305 98 372 4.10e-78 243
MS.gene064111.t1 MTR_3g034180 50.859 291 114 9 28 305 212 486 9.06e-77 243
MS.gene064111.t1 MTR_4g117780 49.825 285 115 7 30 305 99 364 1.23e-76 238
MS.gene064111.t1 MTR_3g034130 52.174 276 100 9 28 289 98 355 3.25e-76 238
MS.gene064111.t1 MTR_4g117770 49.826 287 116 7 28 305 97 364 4.73e-75 234
MS.gene064111.t1 MTR_4g117810 49.826 287 116 7 28 305 97 364 4.73e-75 234
MS.gene064111.t1 MTR_4g122850 50.512 293 111 13 23 301 85 357 1.49e-74 233
MS.gene064111.t1 MTR_8g433030 46.177 327 121 10 3 303 40 337 2.09e-74 232
MS.gene064111.t1 MTR_8g433040 49.296 284 109 7 29 304 104 360 2.39e-74 233
MS.gene064111.t1 MTR_8g032380 49.296 284 109 7 29 304 104 360 2.39e-74 233
MS.gene064111.t1 MTR_4g118380 50.186 269 102 7 29 283 107 357 2.95e-74 232
MS.gene064111.t1 MTR_5g097210 49.485 291 116 11 23 304 91 359 1.00e-73 231
MS.gene064111.t1 MTR_4g109270 49.135 289 113 9 28 305 96 361 1.24e-73 231
MS.gene064111.t1 MTR_4g117870 47.857 280 92 9 28 304 35 263 1.90e-73 227
MS.gene064111.t1 MTR_5g070080 48.264 288 114 10 28 303 116 380 2.59e-70 223
MS.gene064111.t1 MTR_5g070088 45.552 281 135 6 28 303 113 380 6.39e-68 217
MS.gene064111.t1 MTR_5g070130 44.599 287 130 7 28 301 99 369 5.37e-67 214
MS.gene064111.t1 MTR_1g055135 46.367 289 125 9 28 304 101 371 5.45e-67 214
MS.gene064111.t1 MTR_1g055195 43.793 290 122 8 28 305 215 475 5.01e-64 209
MS.gene064111.t1 MTR_4g118400 44.765 277 105 8 29 301 61 293 1.52e-63 202
MS.gene064111.t1 MTR_4g116330 45.455 275 88 7 36 305 121 338 2.23e-63 204
MS.gene064111.t1 MTR_4g116320 45.806 310 98 7 3 305 9 255 3.00e-63 201
MS.gene064111.t1 MTR_4g121580 47.826 276 89 11 33 298 109 339 1.40e-62 203
MS.gene064111.t1 MTR_6g463430 55.446 202 69 4 108 305 15 199 1.96e-60 191
MS.gene064111.t1 MTR_4g117750 44.637 289 128 9 29 304 86 355 7.95e-58 193
MS.gene064111.t1 MTR_4g117750 53.333 120 42 2 188 305 373 480 4.78e-27 110
MS.gene064111.t1 MTR_4g118370 43.130 262 105 10 51 305 33 257 7.07e-56 181
MS.gene064111.t1 MTR_8g040935 51.208 207 80 5 107 305 12 205 1.04e-55 179
MS.gene064111.t1 MTR_7g079400 41.754 285 133 10 33 303 67 332 2.93e-55 182
MS.gene064111.t1 MTR_7g079370 41.463 287 134 8 33 301 79 349 3.83e-55 183
MS.gene064111.t1 MTR_4g117530 39.803 304 106 8 3 305 23 250 3.38e-50 167
MS.gene064111.t1 MTR_1g055165 39.007 282 99 8 30 305 101 315 1.05e-46 160
MS.gene064111.t1 MTR_4g114700 40.441 272 87 7 39 298 9 217 2.17e-46 157
MS.gene064111.t1 MTR_8g102520 35.629 334 154 12 3 301 8 315 3.85e-46 158
MS.gene064111.t1 MTR_8g013010 50.857 175 73 5 28 197 143 309 1.18e-45 157
MS.gene064111.t1 MTR_1g055250 36.977 311 118 12 3 302 21 264 6.85e-43 150
MS.gene064111.t1 MTR_1g055175 43.318 217 92 8 101 305 1 198 1.02e-40 140
MS.gene064111.t1 MTR_4g117430 51.656 151 58 6 50 197 14 152 7.71e-39 134
MS.gene064111.t1 MTR_4g116340 41.395 215 68 6 3 162 36 247 2.98e-38 135
MS.gene064111.t1 MTR_2g048580 36.170 282 128 12 30 301 96 335 3.32e-37 135
MS.gene064111.t1 MTR_7g079420 34.657 277 122 11 38 300 905 1136 6.18e-33 129
MS.gene064111.t1 MTR_7g117720 44.068 177 47 4 3 129 10 184 1.53e-32 121
MS.gene064111.t1 MTR_4g122860 35.161 310 124 14 3 301 70 313 1.99e-32 123
MS.gene064111.t1 MTR_5g023000 55.140 107 34 2 201 305 2 96 2.31e-27 102
MS.gene064111.t1 MTR_4g118360 49.600 125 43 3 185 305 15 123 2.62e-27 103
MS.gene064111.t1 MTR_8g098830 39.326 178 83 8 109 279 35 194 2.84e-25 101
MS.gene064111.t1 MTR_4g023430 46.970 132 39 4 179 305 114 219 4.86e-25 100
MS.gene064111.t1 MTR_7g079450 41.611 149 65 6 156 301 1 130 1.46e-23 94.0
MS.gene064111.t1 MTR_4g133170 30.472 233 138 9 32 247 86 311 3.35e-22 95.9
MS.gene064111.t1 MTR_6g034720 43.902 123 62 3 57 173 48 169 5.66e-22 92.0
MS.gene064111.t1 MTR_4g108820 30.337 267 126 12 3 228 42 289 4.70e-21 91.7
MS.gene064111.t1 MTR_2g028130 28.936 235 133 10 36 243 106 333 6.00e-21 92.4
MS.gene064111.t1 MTR_0667s0010 29.412 238 131 10 36 243 94 324 7.71e-21 92.0
MS.gene064111.t1 MTR_2g028140 30.417 240 128 11 36 243 107 339 2.62e-20 90.9
MS.gene064111.t1 MTR_1g068875 29.018 224 137 7 36 243 91 308 3.79e-20 90.1
MS.gene064111.t1 MTR_1g094210 25.296 253 160 7 32 280 150 377 9.29e-20 89.4
MS.gene064111.t1 MTR_2g426070 30.603 232 132 9 36 243 105 331 3.91e-19 87.4
MS.gene064111.t1 MTR_1g094210 25.610 246 154 7 32 273 150 370 5.27e-19 87.0
MS.gene064111.t1 MTR_1g068835 27.626 257 156 8 36 277 91 332 7.88e-19 86.3
MS.gene064111.t1 MTR_1g097560 25.362 276 170 9 32 300 127 373 7.88e-19 86.3
MS.gene064111.t1 MTR_2g024100 29.218 243 127 11 36 243 96 328 1.05e-18 85.9
MS.gene064111.t1 MTR_4g023100 44.643 112 55 2 200 305 6 116 1.79e-18 80.1
MS.gene064111.t1 MTR_2g025420 28.112 249 132 11 32 243 102 340 2.69e-18 85.1
MS.gene064111.t1 MTR_2g021050 25.474 369 178 15 2 304 4 341 2.88e-18 84.7
MS.gene064111.t1 MTR_4g070550 30.275 218 115 10 36 223 101 311 2.89e-18 84.7
MS.gene064111.t1 MTR_2g028160 29.200 250 133 12 31 244 95 336 3.41e-18 84.7
MS.gene064111.t1 MTR_1g097490 26.007 273 176 8 32 300 140 390 3.51e-18 84.3
MS.gene064111.t1 MTR_3g083010 28.571 238 134 10 36 243 108 339 1.68e-17 82.8
MS.gene064111.t1 MTR_2g426020 29.870 231 134 9 36 244 100 324 1.75e-17 82.4
MS.gene064111.t1 MTR_2g021155 31.364 220 132 10 36 244 130 341 2.42e-17 82.8
MS.gene064111.t1 MTR_2g021155 28.516 256 153 12 3 246 671 908 1.34e-15 77.8
MS.gene064111.t1 MTR_2g426090 28.139 231 139 8 36 242 100 327 2.51e-17 82.0
MS.gene064111.t1 MTR_5g086590 30.348 201 125 8 45 236 9 203 3.06e-17 79.7
MS.gene064111.t1 MTR_2g031640 30.222 225 137 6 36 243 92 313 3.08e-17 81.6
MS.gene064111.t1 MTR_1g068860 30.000 230 130 11 36 243 91 311 3.45e-17 81.6
MS.gene064111.t1 MTR_3g023930 27.306 271 157 11 37 300 130 367 6.41e-17 80.5
MS.gene064111.t1 MTR_2g046920 30.085 236 140 10 32 254 117 340 8.37e-17 80.5
MS.gene064111.t1 MTR_2g055970 26.531 294 183 13 16 300 291 560 2.25e-16 79.7
MS.gene064111.t1 MTR_7g017170 30.047 213 124 11 36 227 124 332 4.79e-16 78.2
MS.gene064111.t1 MTR_7g017130 30.047 213 124 11 36 227 124 332 5.14e-16 78.2
MS.gene064111.t1 MTR_3g009310 27.237 257 159 9 49 300 157 390 5.90e-16 77.8
MS.gene064111.t1 MTR_8g467140 28.829 222 138 9 16 230 112 320 5.93e-16 77.4
MS.gene064111.t1 MTR_5g097350 24.427 262 164 8 50 301 146 383 8.39e-16 77.4
MS.gene064111.t1 MTR_2g031720 28.509 228 147 6 31 243 91 317 8.40e-16 77.4
MS.gene064111.t1 MTR_2g046830 28.270 237 145 10 32 261 118 336 8.59e-16 77.4
MS.gene064111.t1 MTR_8g101580 24.324 370 190 14 2 300 44 394 1.04e-15 77.4
MS.gene064111.t1 MTR_4g107820 24.668 377 180 15 2 300 35 385 1.58e-15 76.6
MS.gene064111.t1 MTR_4g107780 24.668 377 180 15 2 300 35 385 1.58e-15 76.6
MS.gene064111.t1 MTR_5g097330 26.333 300 184 9 25 305 122 403 1.65e-15 76.6
MS.gene064111.t1 MTR_4g107820 24.668 377 180 15 2 300 35 385 2.29e-15 76.3
MS.gene064111.t1 MTR_4g107780 24.668 377 180 15 2 300 35 385 2.37e-15 76.3
MS.gene064111.t1 MTR_1g055200 50.617 81 38 2 35 115 5 83 2.63e-15 70.9
MS.gene064111.t1 MTR_2g021150 29.500 200 129 8 34 228 113 305 2.79e-15 75.9
MS.gene064111.t1 MTR_2g047832 25.882 255 148 10 32 261 122 360 3.01e-15 75.9
MS.gene064111.t1 MTR_8g063440 28.713 202 127 8 40 227 120 318 3.76e-15 75.5
MS.gene064111.t1 MTR_6g021880 28.111 217 138 6 37 242 122 331 4.29e-15 75.5
MS.gene064111.t1 MTR_5g057790 31.604 212 120 11 36 237 142 338 4.97e-15 75.1
MS.gene064111.t1 MTR_3g036290 30.097 206 117 9 34 234 117 300 7.35e-15 74.7
MS.gene064111.t1 MTR_8g013005 62.500 56 19 1 252 305 1 56 8.93e-15 68.2
MS.gene064111.t1 MTR_2g028110 23.885 314 162 8 2 243 3 311 1.79e-14 73.6
MS.gene064111.t1 MTR_4g117550 25.763 295 144 12 39 305 126 373 1.88e-14 73.6
MS.gene064111.t1 MTR_1g097330 28.571 203 130 7 33 229 106 299 2.13e-14 73.6
MS.gene064111.t1 MTR_1g097370 25.088 283 149 12 3 261 68 311 2.58e-14 73.6
MS.gene064111.t1 MTR_2g026010 30.172 232 132 11 36 243 90 315 3.61e-14 72.8
MS.gene064111.t1 MTR_6g079810 26.201 229 131 8 36 227 171 398 3.72e-14 72.8
MS.gene064111.t1 MTR_2g022000 26.282 312 168 14 30 297 114 407 4.21e-14 72.8
MS.gene064111.t1 MTR_2g104340 25.743 303 165 11 40 305 117 396 5.73e-14 72.0
MS.gene064111.t1 MTR_4g030830 24.739 287 177 10 1 261 76 349 5.97e-14 72.0
MS.gene064111.t1 MTR_7g070830 28.916 249 123 12 34 234 114 356 6.39e-14 72.0
MS.gene064111.t1 MTR_3g014540 29.643 280 163 15 36 303 123 380 7.03e-14 71.6
MS.gene064111.t1 MTR_3g010920 26.333 300 179 13 7 300 118 381 1.42e-13 70.9
MS.gene064111.t1 MTR_1g050542 23.741 278 186 11 32 300 144 404 1.51e-13 70.9
MS.gene064111.t1 MTR_3g036430 23.580 352 186 12 3 300 20 342 1.61e-13 70.5
MS.gene064111.t1 MTR_3g030240 26.378 254 150 10 51 300 157 377 5.33e-13 69.3
MS.gene064111.t1 MTR_1g053510 27.004 237 126 10 30 227 113 341 5.80e-13 69.3
MS.gene064111.t1 MTR_0129s0070 24.746 295 171 11 40 305 124 396 6.92e-13 68.9
MS.gene064111.t1 MTR_3g013770 27.402 281 171 10 34 303 132 390 1.01e-12 68.6
MS.gene064111.t1 MTR_5g069120 45.946 74 31 1 33 97 83 156 1.07e-12 65.9
MS.gene064111.t1 MTR_2g031680 27.232 224 143 7 36 243 91 310 1.13e-12 68.2
MS.gene064111.t1 MTR_7g070880 29.412 238 114 12 34 223 114 345 1.16e-12 68.2
MS.gene064111.t1 MTR_4g130330 24.667 300 178 11 36 303 106 389 1.18e-12 68.2
MS.gene064111.t1 MTR_7g017040 25.574 305 177 16 32 305 113 398 1.53e-12 67.8
MS.gene064111.t1 MTR_6g065980 25.271 277 165 12 37 300 111 358 1.65e-12 67.8
MS.gene064111.t1 MTR_7g009265 26.641 259 152 10 33 261 30 280 1.83e-12 67.4
MS.gene064111.t1 MTR_3g007360 28.772 285 158 17 36 301 145 403 2.07e-12 67.4
MS.gene064111.t1 MTR_5g072060 29.688 256 132 14 35 270 109 336 2.55e-12 67.8
MS.gene064111.t1 MTR_0054s0100 25.926 243 144 7 13 223 101 339 2.76e-12 67.0
MS.gene064111.t1 MTR_3g013740 28.218 202 129 9 36 228 149 343 3.32e-12 67.0
MS.gene064111.t1 MTR_3g009300 25.588 340 184 16 6 300 37 352 4.04e-12 66.2
MS.gene064111.t1 MTR_2g078870 26.148 283 168 16 31 300 53 307 4.17e-12 66.2
MS.gene064111.t1 MTR_5g022420 25.000 296 135 12 3 230 27 303 4.21e-12 66.6
MS.gene064111.t1 MTR_2g066170 26.415 212 140 6 27 223 107 317 4.59e-12 66.2
MS.gene064111.t1 MTR_2g021800 24.444 315 178 11 30 301 115 412 4.76e-12 66.6
MS.gene064111.t1 MTR_7g445890 23.698 384 194 14 3 302 16 384 4.90e-12 66.2
MS.gene064111.t1 MTR_7g057270 26.538 260 147 11 34 261 123 370 4.94e-12 66.2
MS.gene064111.t1 MTR_8g063370 26.455 189 126 3 44 222 134 319 9.04e-12 65.5
MS.gene064111.t1 MTR_7g069550 29.057 265 135 13 36 270 123 364 9.16e-12 65.5
MS.gene064111.t1 MTR_2g021770 26.855 283 160 10 53 305 142 407 1.07e-11 65.5
MS.gene064111.t1 MTR_3g006570 26.259 278 172 11 37 300 118 376 1.22e-11 65.1
MS.gene064111.t1 MTR_6g017305 27.136 199 124 7 40 223 5 197 1.35e-11 64.3
MS.gene064111.t1 MTR_3g011060 26.277 274 166 10 34 299 140 385 1.44e-11 65.1
MS.gene064111.t1 MTR_2g021190 26.210 248 159 10 34 275 116 345 1.67e-11 64.7
MS.gene064111.t1 MTR_4g028750 26.941 219 120 7 36 223 120 329 1.75e-11 64.7
MS.gene064111.t1 MTR_5g011200 27.586 203 124 8 36 228 117 306 1.85e-11 64.7
MS.gene064111.t1 MTR_5g037680 23.684 228 141 7 42 261 134 336 2.20e-11 64.7
MS.gene064111.t1 MTR_3g083660 26.549 226 127 9 3 228 25 211 2.21e-11 63.5
MS.gene064111.t1 MTR_7g078900 25.086 291 172 10 33 295 122 394 2.22e-11 64.3
MS.gene064111.t1 MTR_4g028660 23.810 294 179 9 36 301 119 395 2.26e-11 64.3
MS.gene064111.t1 MTR_4g027450 23.810 294 179 9 36 301 119 395 2.26e-11 64.3
MS.gene064111.t1 MTR_7g062060 25.085 295 179 13 33 302 124 401 2.70e-11 64.3
MS.gene064111.t1 MTR_3g007430 26.852 216 131 9 27 227 125 328 3.03e-11 63.9
MS.gene064111.t1 MTR_3g007410 28.421 285 159 17 36 301 145 403 3.13e-11 63.9
MS.gene064111.t1 MTR_4g013180 25.284 352 154 12 4 261 12 348 3.56e-11 63.9
MS.gene064111.t1 MTR_4g028680 25.168 298 174 12 36 303 120 398 3.62e-11 63.9
MS.gene064111.t1 MTR_7g034310 26.639 244 130 12 77 302 17 229 3.63e-11 62.4
MS.gene064111.t1 MTR_4g031770 25.116 215 143 7 22 228 86 290 3.79e-11 63.5
MS.gene064111.t1 MTR_3g083640 24.839 310 188 16 5 300 124 402 4.00e-11 63.5
MS.gene064111.t1 MTR_2g046900 26.639 244 148 12 32 261 121 347 4.07e-11 63.5
MS.gene064111.t1 MTR_3g010390 25.234 214 138 8 36 234 136 342 4.10e-11 63.5
MS.gene064111.t1 MTR_4g013175 25.564 266 145 7 34 261 121 371 4.69e-11 63.5
MS.gene064111.t1 MTR_4g030760 25.620 242 145 10 30 261 170 386 5.43e-11 63.2
MS.gene064111.t1 MTR_5g057690 29.646 226 119 12 34 250 140 334 5.76e-11 63.2
MS.gene064111.t1 MTR_3g007330 23.948 309 192 13 11 300 108 392 5.90e-11 63.2
MS.gene064111.t1 MTR_2g066200 26.786 224 130 8 52 260 137 341 6.79e-11 62.8
MS.gene064111.t1 MTR_4g019990 23.664 262 165 9 22 261 96 344 6.81e-11 62.8
MS.gene064111.t1 MTR_7g055830 26.171 363 190 17 3 303 13 359 7.25e-11 62.8
MS.gene064111.t1 MTR_6g078010 26.316 209 121 8 36 222 68 265 8.90e-11 62.4
MS.gene064111.t1 MTR_5g069390 23.810 294 179 11 36 305 145 417 9.15e-11 62.8
MS.gene064111.t1 MTR_1g055065 26.484 219 133 10 28 229 13 220 9.22e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064111.t1 AT4G12560 27.243 301 178 12 31 304 88 374 3.04e-20 90.5
MS.gene064111.t1 AT4G12560 27.243 301 178 12 31 304 88 374 3.04e-20 90.5
MS.gene064111.t1 AT4G12560 27.243 301 178 12 31 304 88 374 3.04e-20 90.5
MS.gene064111.t1 AT3G06240 26.490 302 181 9 10 300 148 419 3.11e-16 79.0
MS.gene064111.t1 AT4G22390 28.326 233 142 11 33 244 90 318 6.71e-13 68.9
MS.gene064111.t1 AT4G22390 28.326 233 142 11 33 244 90 318 6.75e-13 68.9

Find 63 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGAATGGATTAACTTTAT+TGG 0.141488 4.3:+75396317 None:intergenic
CATTCTGATTTGTGGGTATT+TGG 0.164291 4.3:-75396045 MS.gene064111:CDS
GAAGCGTATATTAATCTATA+TGG 0.223056 4.3:-75396387 MS.gene064111:CDS
TTGGAGAATTTCTCAGTCTA+TGG 0.269491 4.3:-75396026 MS.gene064111:CDS
GGTGATATTATTGGAAAATA+TGG 0.274829 4.3:-75395826 MS.gene064111:CDS
CCTATGTATTCTACAGAAAA+AGG 0.278205 4.3:-75395847 MS.gene064111:CDS
CTTCCTCAGCATGTCATTCC+TGG 0.289129 4.3:-75395919 MS.gene064111:CDS
TCAAATTCTGTTGAGGTTAC+CGG 0.316216 4.3:-75396757 MS.gene064111:CDS
GTAATGATACAATTGAAATA+TGG 0.328111 4.3:-75395990 MS.gene064111:CDS
TATTGTTGGCTTTGATTTAA+CGG 0.336906 4.3:-75396124 MS.gene064111:CDS
TTCGCGTCGTGATTTAGTTT+TGG 0.338685 4.3:-75396151 MS.gene064111:CDS
GTGAAAGTAGTCCGCAAATC+TGG 0.357133 4.3:+75395870 None:intergenic
GTCATCCAAACATTTACATT+TGG 0.370263 4.3:-75396461 MS.gene064111:CDS
GAGGGTAATCACTTACCTTA+TGG 0.385311 4.3:-75396231 MS.gene064111:CDS
TATATTAATCTATATGGGTT+TGG 0.387173 4.3:-75396381 MS.gene064111:CDS
AGTCTATGGGTTAAGGATAC+TGG 0.398340 4.3:-75396012 MS.gene064111:CDS
TGGTGTTCCTCAATCACATT+TGG 0.406622 4.3:-75396292 MS.gene064111:CDS
ATTTCTCAGTCTATGGGTTA+AGG 0.409114 4.3:-75396019 MS.gene064111:CDS
GCTCTGTGATCAACATCATT+TGG 0.411538 4.3:+75396075 None:intergenic
GGATTCCAAATGTAAATGTT+TGG 0.413894 4.3:+75396456 None:intergenic
AAAGTTAATCCATTCTTAGT+TGG 0.417793 4.3:-75396312 MS.gene064111:CDS
ATTAGTACATTCTGATTTGT+GGG 0.419840 4.3:-75396052 MS.gene064111:CDS
AAGCGTATATTAATCTATAT+GGG 0.430207 4.3:-75396386 MS.gene064111:CDS
CATTAGTACATTCTGATTTG+TGG 0.439657 4.3:-75396053 MS.gene064111:CDS
ACATGACCCTTTAATTTCAA+AGG 0.452023 4.3:+75396506 None:intergenic
CATGACCCTTTAATTTCAAA+GGG 0.458275 4.3:+75396507 None:intergenic
GAGTCTCTACTTTCACTCTC+TGG 0.466024 4.3:-75395712 MS.gene064111:CDS
TCTCGTTTAGAGACAATACT+TGG 0.472530 4.3:-75396263 MS.gene064111:CDS
TGATCAACATCATTTGGAAC+AGG 0.489346 4.3:+75396081 None:intergenic
TCTCTATAATCGTCGCCATA+AGG 0.490230 4.3:+75396216 None:intergenic
AATACAAAGTGCATTCGTCT+TGG 0.494278 4.3:-75395957 MS.gene064111:CDS
TGGAGAATTTCTCAGTCTAT+GGG 0.497173 4.3:-75396025 MS.gene064111:CDS
TGAGGAACACCAACTAAGAA+TGG 0.498401 4.3:+75396303 None:intergenic
TACATTTGGAATCCATCTAC+CGG 0.501062 4.3:-75396447 MS.gene064111:CDS
TAATGATACAATTGAAATAT+GGG 0.505203 4.3:-75395989 MS.gene064111:CDS
ATATAGATTAATATACGCTT+CGG 0.513373 4.3:+75396389 None:intergenic
GAAATTAAAGGGTCATGTAG+AGG 0.538383 4.3:-75396501 MS.gene064111:CDS
AAAATATGGTGGTACTAGAT+TGG 0.540379 4.3:-75395812 MS.gene064111:CDS
TGATCATTCAAATTCTGTTG+AGG 0.546882 4.3:-75396764 MS.gene064111:CDS
TCTGTACTATTCCAGATTTG+CGG 0.550677 4.3:-75395881 MS.gene064111:CDS
AAATTAAAGGGTCATGTAGA+GGG 0.550758 4.3:-75396500 MS.gene064111:CDS
ACGATTATAGAGAAGGAGAA+GGG 0.565105 4.3:-75396206 MS.gene064111:CDS
AAATGTAAATGTTTGGATGA+CGG 0.569173 4.3:+75396463 None:intergenic
TTATAGAGAAGGAGAAGGGG+TGG 0.585026 4.3:-75396202 MS.gene064111:CDS
TTGGCTTTGATTTAACGGAA+AGG 0.585773 4.3:-75396119 MS.gene064111:CDS
GACGATTATAGAGAAGGAGA+AGG 0.587454 4.3:-75396207 MS.gene064111:CDS
GATATTATTGGAAAATATGG+TGG 0.590332 4.3:-75395823 MS.gene064111:CDS
TTCGGAAGGACTGTTGCAGA+AGG 0.591659 4.3:+75395750 None:intergenic
ATTTGAATGATCAGTTCTTG+AGG 0.594660 4.3:+75396774 None:intergenic
TATGGCGACGATTATAGAGA+AGG 0.601035 4.3:-75396213 MS.gene064111:CDS
TCGGAATTAAAAGCAGACAT+TGG 0.601452 4.3:+75396408 None:intergenic
CTCTGTATACACGACAACTT+CGG 0.602313 4.3:+75395732 None:intergenic
GTCTAGAGATGATTATGTGG+TGG 0.603968 4.3:-75396349 MS.gene064111:CDS
CAAATCTGGAATAGTACAGA+GGG 0.610389 4.3:+75395884 None:intergenic
GCAAATCTGGAATAGTACAG+AGG 0.612314 4.3:+75395883 None:intergenic
CAATACTTGGAAAGAAATCG+AGG 0.619297 4.3:-75396250 MS.gene064111:CDS
CGATTATAGAGAAGGAGAAG+GGG 0.628339 4.3:-75396205 MS.gene064111:CDS
TGACCAGGAATGACATGCTG+AGG 0.635899 4.3:+75395916 None:intergenic
GTATACACGACAACTTCGGA+AGG 0.636945 4.3:+75395736 None:intergenic
TTGGTGAAGTATAATGACAA+AGG 0.639076 4.3:-75395793 MS.gene064111:CDS
AGAGGGTTAAAGTAGTGACC+AGG 0.655673 4.3:+75395901 None:intergenic
AATACTTGGAAAGAAATCGA+GGG 0.666838 4.3:-75396249 MS.gene064111:CDS
TCAGTCTAGAGATGATTATG+TGG 0.684477 4.3:-75396352 MS.gene064111:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGATACAATTGAAATAT+GGG - chr4.3:75396496-75396515 MS.gene064111:CDS 15.0%
!! AAGCGTATATTAATCTATAT+GGG - chr4.3:75396099-75396118 MS.gene064111:CDS 20.0%
!! ATATAGATTAATATACGCTT+CGG + chr4.3:75396099-75396118 None:intergenic 20.0%
!! GTAATGATACAATTGAAATA+TGG - chr4.3:75396495-75396514 MS.gene064111:CDS 20.0%
!! TATATTAATCTATATGGGTT+TGG - chr4.3:75396104-75396123 MS.gene064111:CDS 20.0%
!!! ATTTTCCCTTTGAAATTAAA+GGG - chr4.3:75395973-75395992 MS.gene064111:CDS 20.0%
!!! TAAGAATGGATTAACTTTAT+TGG + chr4.3:75396171-75396190 None:intergenic 20.0%
!!! TATTTTCCCTTTGAAATTAA+AGG - chr4.3:75395972-75395991 MS.gene064111:CDS 20.0%
! AAAAATATAAGAGACTTCAC+CGG + chr4.3:75395750-75395769 None:intergenic 25.0%
! AAAGTTAATCCATTCTTAGT+TGG - chr4.3:75396173-75396192 MS.gene064111:CDS 25.0%
! AAATCTATAGATCGAATTTC+AGG + chr4.3:75395891-75395910 None:intergenic 25.0%
! AAATGTAAATGTTTGGATGA+CGG + chr4.3:75396025-75396044 None:intergenic 25.0%
! ACAGAAAAAGGTGATATTAT+TGG - chr4.3:75396650-75396669 MS.gene064111:intron 25.0%
! GAAGCGTATATTAATCTATA+TGG - chr4.3:75396098-75396117 MS.gene064111:CDS 25.0%
! GATATTATTGGAAAATATGG+TGG - chr4.3:75396662-75396681 MS.gene064111:intron 25.0%
! GGTGATATTATTGGAAAATA+TGG - chr4.3:75396659-75396678 MS.gene064111:intron 25.0%
!! ATTAGTACATTCTGATTTGT+GGG - chr4.3:75396433-75396452 MS.gene064111:CDS 25.0%
!! CTTTTTCTGTAGAATACATA+GGG + chr4.3:75396640-75396659 None:intergenic 25.0%
!!! TATTGTTGGCTTTGATTTAA+CGG - chr4.3:75396361-75396380 MS.gene064111:CDS 25.0%
!!! TTAGTTTTGGATGTTATTGT+TGG - chr4.3:75396347-75396366 MS.gene064111:CDS 25.0%
AAAAACTCCAAATGTGATTG+AGG + chr4.3:75396203-75396222 None:intergenic 30.0%
AAAATATGGTGGTACTAGAT+TGG - chr4.3:75396673-75396692 MS.gene064111:intron 30.0%
AAATTAAAGGGTCATGTAGA+GGG - chr4.3:75395985-75396004 MS.gene064111:CDS 30.0%
AATACTTGGAAAGAAATCGA+GGG - chr4.3:75396236-75396255 MS.gene064111:CDS 30.0%
ATTTGAATGATCAGTTCTTG+AGG + chr4.3:75395714-75395733 None:intergenic 30.0%
CCTATGTATTCTACAGAAAA+AGG - chr4.3:75396638-75396657 MS.gene064111:intron 30.0%
GGATTCCAAATGTAAATGTT+TGG + chr4.3:75396032-75396051 None:intergenic 30.0%
GTCATCCAAACATTTACATT+TGG - chr4.3:75396024-75396043 MS.gene064111:CDS 30.0%
TCAAATGCGTTTGTAAGTTA+TGG - chr4.3:75395769-75395788 MS.gene064111:CDS 30.0%
TGATCATTCAAATTCTGTTG+AGG - chr4.3:75395721-75395740 MS.gene064111:CDS 30.0%
TTGGTGAAGTATAATGACAA+AGG - chr4.3:75396692-75396711 MS.gene064111:intron 30.0%
! AATGACAAAGGACAGTTTTT+AGG - chr4.3:75396704-75396723 MS.gene064111:intron 30.0%
! ACATGACCCTTTAATTTCAA+AGG + chr4.3:75395982-75396001 None:intergenic 30.0%
! ATGACAAAGGACAGTTTTTA+GGG - chr4.3:75396705-75396724 MS.gene064111:intron 30.0%
! CATGACCCTTTAATTTCAAA+GGG + chr4.3:75395981-75396000 None:intergenic 30.0%
! CATTAGTACATTCTGATTTG+TGG - chr4.3:75396432-75396451 MS.gene064111:CDS 30.0%
! CCTTTTTCTGTAGAATACAT+AGG + chr4.3:75396641-75396660 None:intergenic 30.0%
! TTTTTAATGGGGCTATTCAT+TGG - chr4.3:75396306-75396325 MS.gene064111:CDS 30.0%
!! AAATGTTGATTTGCAAAGTG+AGG + chr4.3:75395813-75395832 None:intergenic 30.0%
AATACAAAGTGCATTCGTCT+TGG - chr4.3:75396528-75396547 MS.gene064111:intron 35.0%
ACGATTATAGAGAAGGAGAA+GGG - chr4.3:75396279-75396298 MS.gene064111:CDS 35.0%
CAATACTTGGAAAGAAATCG+AGG - chr4.3:75396235-75396254 MS.gene064111:CDS 35.0%
GAAATTAAAGGGTCATGTAG+AGG - chr4.3:75395984-75396003 MS.gene064111:CDS 35.0%
TCAAAGGGAAAATAAGAGTC+TGG + chr4.3:75395966-75395985 None:intergenic 35.0%
TCAAATTCTGTTGAGGTTAC+CGG - chr4.3:75395728-75395747 MS.gene064111:CDS 35.0%
TCAGTCTAGAGATGATTATG+TGG - chr4.3:75396133-75396152 MS.gene064111:CDS 35.0%
TCGGAATTAAAAGCAGACAT+TGG + chr4.3:75396080-75396099 None:intergenic 35.0%
TCTCGTTTAGAGACAATACT+TGG - chr4.3:75396222-75396241 MS.gene064111:CDS 35.0%
TCTGTACTATTCCAGATTTG+CGG - chr4.3:75396604-75396623 MS.gene064111:intron 35.0%
TGGAGAATTTCTCAGTCTAT+GGG - chr4.3:75396460-75396479 MS.gene064111:CDS 35.0%
TTGGAGAATTTCTCAGTCTA+TGG - chr4.3:75396459-75396478 MS.gene064111:CDS 35.0%
! ATTTCTCAGTCTATGGGTTA+AGG - chr4.3:75396466-75396485 MS.gene064111:CDS 35.0%
! CAAATCTGGAATAGTACAGA+GGG + chr4.3:75396604-75396623 None:intergenic 35.0%
! CATTCTGATTTGTGGGTATT+TGG - chr4.3:75396440-75396459 MS.gene064111:CDS 35.0%
! TACATTTGGAATCCATCTAC+CGG - chr4.3:75396038-75396057 MS.gene064111:CDS 35.0%
! TGATCAACATCATTTGGAAC+AGG + chr4.3:75396407-75396426 None:intergenic 35.0%
! TGGTATTTGTCTTTTCGATC+CGG + chr4.3:75396060-75396079 None:intergenic 35.0%
!! TTGGCTTTGATTTAACGGAA+AGG - chr4.3:75396366-75396385 MS.gene064111:CDS 35.0%
AGGGAAAATAAGAGTCTGGA+AGG + chr4.3:75395962-75395981 None:intergenic 40.0%
CGATTATAGAGAAGGAGAAG+GGG - chr4.3:75396280-75396299 MS.gene064111:CDS 40.0%
CTCTGTATACACGACAACTT+CGG + chr4.3:75396756-75396775 None:intergenic 40.0%
GACGATTATAGAGAAGGAGA+AGG - chr4.3:75396278-75396297 MS.gene064111:CDS 40.0%
GAGGGTAATCACTTACCTTA+TGG - chr4.3:75396254-75396273 MS.gene064111:CDS 40.0%
GCTCTGTGATCAACATCATT+TGG + chr4.3:75396413-75396432 None:intergenic 40.0%
GGAAGGGAACAGTTAAAGTT+GGG + chr4.3:75395945-75395964 None:intergenic 40.0%
GGGAAAATAAGAGTCTGGAA+GGG + chr4.3:75395961-75395980 None:intergenic 40.0%
GTCTAGAGATGATTATGTGG+TGG - chr4.3:75396136-75396155 MS.gene064111:CDS 40.0%
TATGGCGACGATTATAGAGA+AGG - chr4.3:75396272-75396291 MS.gene064111:CDS 40.0%
TCTCTATAATCGTCGCCATA+AGG + chr4.3:75396272-75396291 None:intergenic 40.0%
TGAGGAACACCAACTAAGAA+TGG + chr4.3:75396185-75396204 None:intergenic 40.0%
TGGAAGGGAACAGTTAAAGT+TGG + chr4.3:75395946-75395965 None:intergenic 40.0%
TGGTGTTCCTCAATCACATT+TGG - chr4.3:75396193-75396212 MS.gene064111:CDS 40.0%
! AGAAGGGGTGGTTTTTAATG+GGG - chr4.3:75396295-75396314 MS.gene064111:CDS 40.0%
! AGTCTATGGGTTAAGGATAC+TGG - chr4.3:75396473-75396492 MS.gene064111:CDS 40.0%
! GAGAAGGGGTGGTTTTTAAT+GGG - chr4.3:75396294-75396313 MS.gene064111:CDS 40.0%
! GCAAATCTGGAATAGTACAG+AGG + chr4.3:75396605-75396624 None:intergenic 40.0%
!! TTCGCGTCGTGATTTAGTTT+TGG - chr4.3:75396334-75396353 MS.gene064111:CDS 40.0%
AGAGGGTTAAAGTAGTGACC+AGG + chr4.3:75396587-75396606 None:intergenic 45.0%
CAGTTAAAGTTGGGTGAAGC+AGG + chr4.3:75395936-75395955 None:intergenic 45.0%
GAGTCTCTACTTTCACTCTC+TGG - chr4.3:75396773-75396792 MS.gene064111:CDS 45.0%
GTATACACGACAACTTCGGA+AGG + chr4.3:75396752-75396771 None:intergenic 45.0%
GTGAAAGTAGTCCGCAAATC+TGG + chr4.3:75396618-75396637 None:intergenic 45.0%
TTATAGAGAAGGAGAAGGGG+TGG - chr4.3:75396283-75396302 MS.gene064111:CDS 45.0%
! GGAGAAGGGGTGGTTTTTAA+TGG - chr4.3:75396293-75396312 MS.gene064111:CDS 45.0%
! TGTCTTTTCGATCCGGTAGA+TGG + chr4.3:75396053-75396072 None:intergenic 45.0%
CTTCCTCAGCATGTCATTCC+TGG - chr4.3:75396566-75396585 MS.gene064111:intron 50.0%
TGACCAGGAATGACATGCTG+AGG + chr4.3:75396572-75396591 None:intergenic 50.0%
TTCGGAAGGACTGTTGCAGA+AGG + chr4.3:75396738-75396757 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 75395702 75396805 75395702 ID=MS.gene064111
chr4.3 mRNA 75395702 75396805 75395702 ID=MS.gene064111.t1;Parent=MS.gene064111
chr4.3 exon 75396727 75396805 75396727 ID=MS.gene064111.t1.exon1;Parent=MS.gene064111.t1
chr4.3 CDS 75396727 75396805 75396727 ID=cds.MS.gene064111.t1;Parent=MS.gene064111.t1
chr4.3 exon 75395702 75396540 75395702 ID=MS.gene064111.t1.exon2;Parent=MS.gene064111.t1
chr4.3 CDS 75395702 75396540 75395702 ID=cds.MS.gene064111.t1;Parent=MS.gene064111.t1
Gene Sequence

>MS.gene064111

ATGGATCTTCCTCAAGAACTGATCATTCAAATTCTGTTGAGGTTACCGGTGAAGTCTCTTATATTTTTCAAATGCGTTTGTAAGTTATGGTTCTCTCTTATCTCTGATCCTCACTTTGCAAATCAACATTTTCAACTTAACTTTGCAAAACACACTCGTAGATTTTTATGCATTTCAGCTCTATCTCCTGAAATTCGATCTATAGATTTTGATGCATTTCTTAACAATGCTCCTGCTTCACCCAACTTTAACTGTTCCCTTCCAGACTCTTATTTTCCCTTTGAAATTAAAGGGTCATGTAGAGGGTTTATATTCTTGTACCGTCATCCAAACATTTACATTTGGAATCCATCTACCGGATCGAAAAGACAAATACCAATGTCTGCTTTTAATTCCGAAGCGTATATTAATCTATATGGGTTTGGTTATGATCAGTCTAGAGATGATTATGTGGTGGTTTTATTGTCCAATAAAGTTAATCCATTCTTAGTTGGTGTTCCTCAATCACATTTGGAGTTTTTCTCGTTTAGAGACAATACTTGGAAAGAAATCGAGGGTAATCACTTACCTTATGGCGACGATTATAGAGAAGGAGAAGGGGTGGTTTTTAATGGGGCTATTCATTGGTTGTCTTCGCGTCGTGATTTAGTTTTGGATGTTATTGTTGGCTTTGATTTAACGGAAAGGAAATTGTTTGAGATGCCTGTTCCAAATGATGTTGATCACAGAGCATTAGTACATTCTGATTTGTGGGTATTTGGAGAATTTCTCAGTCTATGGGTTAAGGATACTGGTAATGATACAATTGAAATATGGGTGATGAAAGAATACAAAGTGCATTCGTCTTGGACTAAAACTCTTGTTCTTCCTCAGCATGTCATTCCTGGTCACTACTTTAACCCTCTGTACTATTCCAGATTTGCGGACTACTTTCACCCTATGTATTCTACAGAAAAAGGTGATATTATTGGAAAATATGGTGGTACTAGATTGGTGAAGTATAATGACAAAGGACAGTTTTTAGGGCATCACTCCTTCTGCAACAGTCCTTCCGAAGTTGTCGTGTATACAGAGTCTCTACTTTCACTCTCTGGTGACAATTAG
Protein sequence

>MS.gene064111.t1

MDLPQELIIQILLRLPVKSLIFFKCVYSYFPFEIKGSCRGFIFLYRHPNIYIWNPSTGSKRQIPMSAFNSEAYINLYGFGYDQSRDDYVVVLLSNKVNPFLVGVPQSHLEFFSFRDNTWKEIEGNHLPYGDDYREGEGVVFNGAIHWLSSRRDLVLDVIVGFDLTERKLFEMPVPNDVDHRALVHSDLWVFGEFLSLWVKDTGNDTIEIWVMKEYKVHSSWTKTLVLPQHVIPGHYFNPLYYSRFADYFHPMYSTEKGDIIGKYGGTRLVKYNDKGQFLGHHSFCNSPSEVVVYTESLLSLSGDN